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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="437" mass="47929" checksum="731DFEBA82368BB2" modified="2011-06-28" version="1">MNSLLALSLILLLLLPLSSISLIHSSAVEDPAYPSGMSFFPLTNVIYTTYVIGTVNITSLNIGNSYLSSGMFFTKGNASLQLNAMIDGKYWAQDVALFHEINNKEFEITMIINLWNLSGPFKILKNNVTTYQGLGVYLYQGPTFNVTLPLNFSLFMNATNNLEFGYCINGKKYVYQVLPYFGNFQIGGLSILGLPNDLEFVWGGPGCGSEVCMSGEMSEEIYYLQQGKLVIPSSAFSVGLDTAESAYGIKVFANFENISSPFACITNGVNSPSVLWPVPPSIIVQSNGSIKHVKLCINGFPLSNQEVEVLVPSANLSSPIPFTTVKELGYTNSNGEITFNISSQSLYIIYYPGNYTLSSAYDISSPLLSHLVSSIKSTYDSLVNFLKSYNFKHALSSSFSRIKYRSQSTSVDYLLLEYIGAFAVGILVSAILAKYKF</sequence>
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            <fullName evidence="9">4Fe-4S ferredoxin, iron-sulfur binding domain protein</fullName>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="STRING" id="933801.Ahos_0011"/>
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    <dbReference type="KEGG" id="aho:Ahos_0011"/>
    <dbReference type="eggNOG" id="arCOG03696">
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    <dbReference type="HOGENOM" id="CLU_022113_0_0_2"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
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    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051684">
        <property type="entry name" value="Electron_Trans/Redox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176:SF3">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe-4S ferredoxins"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="6"/>
            <end position="27"/>
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    </feature>
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        </location>
    </feature>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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</entry>
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        </recommendedName>
    </protein>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N4"/>
    <dbReference type="STRING" id="933801.Ahos_0028"/>
    <dbReference type="GeneID" id="10599468"/>
    <dbReference type="KEGG" id="aho:Ahos_0028"/>
    <dbReference type="eggNOG" id="arCOG07312">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="54"/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="SMART" id="SM00933"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="335" mass="37969" checksum="01EED6D237963059" modified="2011-06-28" version="1">MNSTDILNKIKKLAEEEDEKARKVDAILSLVAEDIYYGNSDLEFLEIDNARNPHIACAVDGSKYQLDVGDFSLIIARAVKVKGIYGEKGKRIPPDIKEDVKLEDNYYGEDKVGKDAILLMLTLETEILSSCSDCNVVFIDGPIIDPPTYDDNDEDLKKFHEVRKLTISTSDKIIGIVKRFAQRFLINKLINDGYAQLIEARESYIVQNLFLKLRQKFEDYKNPRFLGWISWDSIFSSNYTTDLAGLGKAYKKYNIKLYSGYFQQNAISPVARIDVLSPDNNKVAYIATWSYPGITEVTILNKLADDISNVSKAEAEAYLNLLISARKTLPMMRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P9</accession>
    <name>F4B3P9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Superoxide dismutase</fullName>
            <ecNumber evidence="3 8">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1203</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="3 8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776002.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P9"/>
    <dbReference type="SMR" id="F4B3P9"/>
    <dbReference type="STRING" id="933801.Ahos_1203"/>
    <dbReference type="GeneID" id="10600679"/>
    <dbReference type="KEGG" id="aho:Ahos_1203"/>
    <dbReference type="eggNOG" id="arCOG04147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_2_2_2"/>
    <dbReference type="OrthoDB" id="32917at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000004">
        <property type="entry name" value="Superoxide dismutase [Fe]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="10">
        <location>
            <begin position="105"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="211" mass="24312" checksum="5A88EFF501E76164" modified="2011-06-28" version="1">MSSLTYLKKYELPPLPYNLDALEPYISKEIIDVHYNGHHRGYVNGANSFVDRVNKILKGEISSGQYDIQGLLRGLVFNINGHKLHSLYWQNMAPAGKGGGKPGGVIGDLIEKQYGSFEKFKALFTEAANSLPGTGWAVLYYEVENGNLQIMTFENHFQNHIAELPILLILDEFEHAYYLQYKNKRADYVNNWWNLVNWDFADKKLQQYMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T1</accession>
    <name>F4B3T1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T1"/>
    <dbReference type="KEGG" id="aho:Ahos_1235"/>
    <dbReference type="eggNOG" id="arCOG07720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600811_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="438" mass="48870" checksum="4F6698CE672BC7F9" modified="2011-06-28" version="1">MRIKENANMGVETSSSLRYLGGIIGTLLEAVITLDCMQENCVKEGLKRYNSMESFQRYEVYPAISAGMRVLKDASSSPERIFRQGIVVKTTDTGDWFYIGGISQYWTSDQLIIYQGGSQASSQGKLNRGIIDDFVNKGGLGVVPLYKERAPSVWYNPVLFKDCQGSFGIFWNYLGEFQAGVLSIFSNAPNILRYTEDLIKAGKASLAYSSYGNYYLSRAAENDVMRPASDNPYVYLALGTNPLVAKSHGLQIYPSFTFDTVTSDVSSCCENIMPEPYCCSYFLKYVRFNDIDIGAPVYATLPCGTSCSTFGLAGLIMGISSMTVNNVQLIYLTIAQPPSDFTTSAIIEWSKTMGFYDSLNKLFEAGKRFKKAITDLSTAFPEFIATAAALTVDWLESYEEGLKRAEVKARELNELWLLTNWLVSRCQQFPIIREVYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X7</accession>
    <name>F4B3X7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774858.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X7"/>
    <dbReference type="STRING" id="933801.Ahos_0046"/>
    <dbReference type="GeneID" id="10599486"/>
    <dbReference type="KEGG" id="aho:Ahos_0046"/>
    <dbReference type="eggNOG" id="arCOG00586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259116_0_0_2"/>
    <dbReference type="OrthoDB" id="36110at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02042">
        <property type="entry name" value="ParAB_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050678">
        <property type="entry name" value="DNA_Partitioning_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696:SF99">
        <property type="entry name" value="COBYRINIC ACID AC-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="219" mass="23840" checksum="35D66AA4D89E5399" modified="2011-06-28" version="1">MIITVINQKGGVGKTTTAVNLSYVLSKSKNVALLDMDPEGGSTTSFGMKREKKELEIGSKSVNIFNVEVFPAHLGLLKAELNGDVEDVVNKIKETAKDFDILVIDTPPNLGTLAVASMIAADKILSPVTPQPLALEAAKNLDGRLQSLNKKALAFTNLSNKAISLELSSVQFLNIHIPQSRLFIEASRLGVPATRYEEVRLKKPKLSHFFEELAKVTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B436</accession>
    <name>F4B436_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0107</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774917.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B436"/>
    <dbReference type="STRING" id="933801.Ahos_0107"/>
    <dbReference type="GeneID" id="10599547"/>
    <dbReference type="KEGG" id="aho:Ahos_0107"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="276" mass="30353" checksum="400A8F79EB22855D" modified="2011-06-28" version="1">MIFTDRLTKVYKDGTRALDELTVTLDGKISCILGRNGAGKTTLIRILSTQLLPTSGKALIEGFDVIKDVKRVRKIIASIPQEASPMGIASPLEHVTMYLTARGFSISEALRTAKYVLKEVGLGDVMDKPTDELSGGMKRKVFVAMAIASNADVVFLDEPTTGLDPISRLEVWSAIKQMKSKIILTTHYMEEAEELCEKIAIINKGKLVEYGTVEDLLSPLRGKVRVEGKGDIRVGNLSLSYVDEEEAKKLVGKGVVIKPISLEDVMIMKGLYYDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B456</accession>
    <name>F4B456_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peroxiredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776080.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B456"/>
    <dbReference type="STRING" id="933801.Ahos_1281"/>
    <dbReference type="GeneID" id="10600762"/>
    <dbReference type="KEGG" id="aho:Ahos_1281"/>
    <dbReference type="eggNOG" id="arCOG02067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121282_0_0_2"/>
    <dbReference type="OrthoDB" id="25864at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032836">
        <property type="entry name" value="DsrE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF3">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13686">
        <property type="entry name" value="DrsE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="14857" checksum="220B8D3F42BD513E" modified="2011-06-28" version="1">MAKLTILLADNSMDKFYHGLVLAIGGKSLNWDVKFFVTSQAVILFTKEMKGKSKLKMGFFARLFISFQMKRMNIPDTSKLLMEAIKEGVEFYVDEAGLKLAGFSKSDLLDGIKLSDTISFLEEAKTSDVVVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B470</accession>
    <name>F4B470_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl11</name>
        <name evidence="10" type="ordered locus">Ahos_1295</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776094.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B470"/>
    <dbReference type="STRING" id="933801.Ahos_1295"/>
    <dbReference type="GeneID" id="10600776"/>
    <dbReference type="KEGG" id="aho:Ahos_1295"/>
    <dbReference type="eggNOG" id="arCOG04372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="OrthoDB" id="8842at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002232">
        <property type="entry name" value="PRK01143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="8">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="168" mass="18091" checksum="F366C839C194559A" modified="2011-06-28" version="1">MPKKTIKIVVEGGNVKPGPPLGPTLSQLKLNVGEVVKKINEATAQFKGMTVPVTIDVDLDTKEFEISVGIPTTSSLLLKKASAEAPSGDPAHKKIGNVSLEDIIDVAITKKPSLTAKTLKAAVKSILGTARQIGLTVDKKDPKELINEIDEGKYDELFSKYEEKWNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B473</accession>
    <name>F4B473_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl12</name>
        <name evidence="6" type="ordered locus">Ahos_1298</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776097.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B473"/>
    <dbReference type="STRING" id="933801.Ahos_1298"/>
    <dbReference type="GeneID" id="10600779"/>
    <dbReference type="KEGG" id="aho:Ahos_1298"/>
    <dbReference type="eggNOG" id="arCOG04287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="OrthoDB" id="3337at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_P1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="105" mass="11133" checksum="940D8EDF1C180B15" modified="2011-06-28" version="1">MEYIYASLLLHSAKKDITEDNLKNVLTAAGVNVDEVRVKAVVAALKEVNIDEVLKNAAAMPVAAAAPAQAQAAPAKEEKKEEKKEEEKKGPSEEEIAGGLSSLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B486</accession>
    <name>F4B486_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776110.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B486"/>
    <dbReference type="GeneID" id="10600795"/>
    <dbReference type="KEGG" id="aho:Ahos_1311"/>
    <dbReference type="eggNOG" id="arCOG05404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2103361_0_0_2"/>
    <dbReference type="OrthoDB" id="57108at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="115" mass="13602" checksum="06D692175CD80F5F" modified="2011-06-28" version="1">MNPKIEEKIAQMRCENRPVKQIAKKLGVNRDDIEAVIKKWISYTNEYLKELVKNRKVKNPKADPGFIVNVTTSVEELLKNDDVLDYIALHMSDYHDRLMDCIRYKVYIYLKQKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B490</accession>
    <name>F4B490_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved archaeal conjugative plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012713836.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B490"/>
    <dbReference type="GeneID" id="58788753"/>
    <dbReference type="KEGG" id="aho:Ahos_1315"/>
    <dbReference type="eggNOG" id="arCOG08462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3021140_0_0_2"/>
    <dbReference type="OrthoDB" id="40549at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="55" mass="6533" checksum="7F78B98B54E4F3C2" modified="2011-06-28" version="1">MSENIKLVRKYLAIDENRNIVAEGNSWEEVEEIMEKKGYKRSQYDILTVVKQEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B497</accession>
    <name>F4B497_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SpoVT/AbrB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B497"/>
    <dbReference type="STRING" id="933801.Ahos_1322"/>
    <dbReference type="KEGG" id="aho:Ahos_1322"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="20"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="334" mass="38030" checksum="1AB07C3370C64D51" modified="2011-06-28" version="1">MTDLEEFDNSKDIETRKVQKLGSSSLFITLPKKWINKWNIKPGDKILIEISDDGSLKLLAEKIKLLSNRKTIKIDIDAIKQPIPSMVLCLYSLGYDEIVFESKKQIVQKEIEDLISTTKQMVGAEVTEASENRVKLECLLDAEKVGLESLLRRILNIISKKVDGIISYLSGEQPKDSELTQQEDLRRVYLMLLRHVIGSKYEVSSNLNKNFLVVMNAVILLNISRLIDRIDSTIKTAKLNEDTPILKEILQKINDLLDEVIMSILFPSMKRVTNGLNLINQINSLLDSFNDKSSIVYTSVVDIISNLQLALENSSCTLFLEDMPWIERNFNVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B499</accession>
    <name>F4B499_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylate--amine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B499"/>
    <dbReference type="STRING" id="933801.Ahos_1324"/>
    <dbReference type="KEGG" id="aho:Ahos_1324"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF3">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27319" checksum="AF46414C3A8491A6" modified="2011-06-28" version="1">MSFEIRESYIPQLTKPSYMIIGLPDAGLVGEIATEFLIEKGGFKEYGEVFSKKYIPPVMHVTNGVAKSPLRVYHNVNTIILHAWTALPASSAYPLAEFIVDYAKKYGIDTIISITGVPIPNRLDIEKPTAYWIASEEGLSEELSNTNLMQKFDDGYIAGPYAPILLEASRKNVRNFVVVVESFLDIPDPEASAVALDIISKYIGFSIDVSSLLKEAEEIRAKIKGLMEQTKKELPSYSSVRPMSYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A2</accession>
    <name>F4B4A2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable glycine dehydrogenase (decarboxylating) subunit 1</fullName>
            <ecNumber evidence="3">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Glycine cleavage system P-protein subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Glycine decarboxylase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Glycine dehydrogenase (aminomethyl-transferring) subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvPA</name>
        <name evidence="5" type="ordered locus">Ahos_1327</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GcvP family. N-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776125.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A2"/>
    <dbReference type="STRING" id="933801.Ahos_1327"/>
    <dbReference type="GeneID" id="10600813"/>
    <dbReference type="KEGG" id="aho:Ahos_1327"/>
    <dbReference type="eggNOG" id="arCOG00077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_0_2_2"/>
    <dbReference type="OrthoDB" id="17655at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00712">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023010">
        <property type="entry name" value="GcvPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001696">
        <property type="entry name" value="PRK00451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM P-PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006815">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="4">
        <location>
            <begin position="13"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="450" mass="51185" checksum="A8B6D72A20813BE9" modified="2011-06-28" version="1">MDYHPWLPNLDYENEMIREIGVNSIDDLFKDIPEEIKLKRLLNVGYSKPLSEYEIYLRLLELENKNAKLRMPPFLGGGVCPHYVPEAVKFVISRSEFYTSYTPYQPEISQGLLQALFEYQSLMADLLEMEVVNSSMYEWGSALAEAVLMAYRINGKKKVVIPASINPYHKKVLETWTYGKEIKIKEIPYTNEGKINLEVAEKEIDEETSAVYIQQPNFFGIFETEIEEIVDLAKKKGAITIMGVNPLSLSLIKPPGEYDIDIAVGDGQELGLPLNFGGPYMGIFATKWDGKLVRQMPGRLVGLTTDSEGKRGYTLILQTREQFTRREKATSNITTNEALMAIANAVYISLLGKSGLRKLAEEIYKRSHYALLEFERKGVGKRKFNSDFFEEFTVSFPISYGKIHENLKARGIYGGLKISDKDAIFCVTEVHTKHMIDEMIDEAKKIVETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C3</accession>
    <name>F4B4C3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="8">IGPS</shortName>
            <ecNumber evidence="8">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpC</name>
        <name evidence="10" type="ordered locus">Ahos_1348</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776146.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C3"/>
    <dbReference type="STRING" id="933801.Ahos_1348"/>
    <dbReference type="GeneID" id="10600834"/>
    <dbReference type="KEGG" id="aho:Ahos_1348"/>
    <dbReference type="eggNOG" id="arCOG01088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_0_1_2"/>
    <dbReference type="OrthoDB" id="15223at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001374">
        <property type="entry name" value="PRK00278.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="248" mass="28264" checksum="5B9CD54E92FAA226" modified="2011-06-28" version="1">MVRFLTGWLKDVVNNAYSRPYVHKIRDKPVFSLIDAIRKVKEENKNPIIAEYKRASPSGFSADRDPIEYAKEMERSGAVGISVLTEDKFFSGSYDYLFIISHTVKIPVLMKDFVVTERQIDNAYNLGADSILLIVRILTERELESLILYARSYRLEPLVEIFDENELEMALNAGAKIIGVNSRDLLTFAIDQERVKRVLRKIPSNVIRVAESGITSKEQIEELKKYGADAFLIGSSLMKEPSKIKELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C7</accession>
    <name>F4B4C7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cellulose synthase, subunit A-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0123</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774933.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C7"/>
    <dbReference type="STRING" id="933801.Ahos_0123"/>
    <dbReference type="GeneID" id="10599563"/>
    <dbReference type="KEGG" id="aho:Ahos_0123"/>
    <dbReference type="eggNOG" id="arCOG01391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011907_4_2_2"/>
    <dbReference type="OrthoDB" id="43988at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06421">
        <property type="entry name" value="CESA_CelA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050321">
        <property type="entry name" value="Glycosyltr_2/OpgH_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867:SF2">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="8">
        <location>
            <begin position="158"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="502" mass="57769" checksum="C8973A8984B9020B" modified="2011-06-28" version="1">MRILNIIYAVIPLFLSSIFTYLTYASIFTLWLAFGVWLSMIVGFFIFIDFYRLSRKSTNYEINYTTNTKKYKIASFVTSFNENPEIVEGTLISVKQATKDYGDVYLLDDSTDQKIAEELKAFCEKYGIIYLHRKDRKGFKAGAINEALRKVGDNYDLVAIFDADQRPTENFFEQVFPYFSDPKVALVQVPQNYSETYSGIARAAKYQQEPFLRIIMRGRTFSSAFSLGSGSVFRISALKEVGYMDESSITEDAATSIKLHEKGYTSVYVDSQLIWYGEPPEDINAYLTQQSRWAFGYFQLTWKILKSNLNFSQFVDYFSGSLYWVKEGPLTIAEFLAPVFFLLFKLPIIILNPLIYILAYIPYLLGSIAQFIYAIKGKGEYGIKGFYYHQSVEYLGFLSITLAFFSWIIGKKRPFKVTPKGEGNFDVKSVIPHMIIIILLILSIIEGFLWLTKASSSLPLYYTIIINIFWAIYQIPFLIGGIILAKMMSRRENYVEVALNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F8</accession>
    <name>F4B4F8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774964.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4F8"/>
    <dbReference type="GeneID" id="10599594"/>
    <dbReference type="KEGG" id="aho:Ahos_0154"/>
    <dbReference type="eggNOG" id="arCOG03708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_1_0_2"/>
    <dbReference type="OrthoDB" id="43833at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="159" mass="18123" checksum="57DF97F53FA273F8" modified="2011-06-28" version="1">MEELPKPWFNMQDYKKARLLEAKYEAEIARRFLEEGLLRNAAGKVYQAWKALVAAFATDYRDKLMQKFKGEVKIRGNKKVQKADWIIAIMPSSLIKAVAQTIGGDIDTYTNIALLLHQYQYNGPDSQAILSQYINDESAKEDILKLLGVIDNILSKVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H1</accession>
    <name>F4B4H1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin/lipoate A/B protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774977.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H1"/>
    <dbReference type="STRING" id="933801.Ahos_0168"/>
    <dbReference type="GeneID" id="10599608"/>
    <dbReference type="KEGG" id="aho:Ahos_0168"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_0_0_2"/>
    <dbReference type="OrthoDB" id="43646at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16443">
        <property type="entry name" value="LplA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="29"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="254" mass="29400" checksum="28A56CE936DC8FFF" modified="2011-06-28" version="1">MRVLFYENDENPYFSLAFEESLWRNLKEGKVDNTLRFWRHKNVAIIGYFQLAEEELNFDVVRKYKIDVVRRFTGGGAVYQDMGCLIWTIAVKGPKDGGVNYLYNFLLRGFVNALKRFANVRVENINDVVVNDRKVSGTSATFQGEYYLLHGTLLINTNLELMGKILKVSKAKLSDKGVSEVKYRVTNLVDALGRKINTSEIIDAIIKEYSSLLGEKAYFDLPTTDEIKLAEELYEKKYSKPEWNLLRLPSRYFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J0</accession>
    <name>F4B4J0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774996.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J0"/>
    <dbReference type="GeneID" id="10599627"/>
    <dbReference type="KEGG" id="aho:Ahos_0187"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="14301" checksum="050F977E78649D74" modified="2011-06-28" version="1">MKKILKSPADIYLEEADELLERGDIVQASEKYYKAAEEAIKLFSRRLNLEPILSEVNKKERWKSEILFKAARLINEKYPEVFKMWKSAWKLHEDGFHECSLDLETARALGEIVKNTLMKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J3</accession>
    <name>F4B4J3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774999.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J3"/>
    <dbReference type="STRING" id="933801.Ahos_0190"/>
    <dbReference type="GeneID" id="10599630"/>
    <dbReference type="KEGG" id="aho:Ahos_0190"/>
    <dbReference type="eggNOG" id="arCOG00877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025659_0_0_2"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050547">
        <property type="entry name" value="DEAD_box_RNA_helicases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963:SF8">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="31"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="283"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="663" mass="75521" checksum="AC4847CC5D5E6A8F" modified="2011-06-28" version="1">MLSDFDKLVKDKLGYNLFPYQSHVSERIIKELEEGKSNFIVVSMPTGSGKTVIELFMAYYFLKKGVKNVIVMEPTRLLCDQMYFNFWRKIFGEDVGMEYEGECTAFEEGKKIVVSTPFTAEKCAPETEAIIVDEVHHAFGDSRYESTLVSLDPKYLIGFTALLPSYKKYMVDPRLVEKLGKPKFLNYDFKALAEIDPTFNPPKAIADVFDAEMNKIEDSAYDAFFRGQVKGDKNTLKFLEITLYSYGKEAFCESLGRMEGKIEENPQLDFLCESKELSHKARALRQILSIYKIEDFKPVLIFTSRKATAYEFEKAIEDLDPGRIKVLTGDSSKFERQKLVKSAKRGEIDVIISTLVGEEGIDIPEAKLLIMTDVPQSPLRFYQRLGRLIRSKTDENNNYSENTLKYLVVALTPKTPEYDNLDDALRNLYLEGVDVSYIVEKKEGKGPVARVVDIVKKEGGGTSFSKLDSSVGEISSIEVLLSPKNVETQMGDYVDRAIKEGRIFYYYDVDKMADLASKVLLGSYCNLCFGEKCMKICKDCIREVGKLLISKKGRVKLEKKGLLLHYMELVLPEKMDEIMNAMNKEKDEIMKKVESAFISLSSTKRANVYSIIINFNVSVDGMTIYPKLQLDYYNANEQEIKLAEINAKAIGYKAIYLFLQQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K2</accession>
    <name>F4B4K2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775008.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K2"/>
    <dbReference type="STRING" id="933801.Ahos_0199"/>
    <dbReference type="GeneID" id="10599639"/>
    <dbReference type="KEGG" id="aho:Ahos_0199"/>
    <dbReference type="eggNOG" id="arCOG03707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_1_0_2"/>
    <dbReference type="OrthoDB" id="27246at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="159" mass="18592" checksum="14CB676E438291FA" modified="2011-06-28" version="1">MVEEIEKPWIDSKKYQEDRFKEALYEADLAERFLNDGLIRNSAGKAYQAVKAYVAGLAINYRDLLSKYFPGKRRLSPRKVVERVDWIIAIMPSTRLREIASIINDDKLRLIVEIAIDLHEFQYNGLDKDSEISRYPNEELVKKDILEVTNFIKSRVKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L0</accession>
    <name>F4B4L0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776155.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L0"/>
    <dbReference type="STRING" id="933801.Ahos_1357"/>
    <dbReference type="GeneID" id="10600844"/>
    <dbReference type="KEGG" id="aho:Ahos_1357"/>
    <dbReference type="eggNOG" id="arCOG07221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171532_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10864" checksum="3278E79BDF76696F" modified="2011-06-28" version="1">MNLCEICEEKEAKYSCKLCGRKVCEDDFVKEKGICKVCEISLCQICHQHLSLGYCEICGRLICDECTAYFDGSRRICKECYAKKVSSKIISSISQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L4</accession>
    <name>F4B4L4_ACIHW</name>
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        <submittedName>
            <fullName evidence="5">UbiD family decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1361</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776159.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L4"/>
    <dbReference type="STRING" id="933801.Ahos_1361"/>
    <dbReference type="GeneID" id="10600848"/>
    <dbReference type="KEGG" id="aho:Ahos_1361"/>
    <dbReference type="eggNOG" id="arCOG01671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_4_1_2"/>
    <dbReference type="OrthoDB" id="8480at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049383">
        <property type="entry name" value="UbiD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
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    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF17">
        <property type="entry name" value="FERULIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20695">
        <property type="entry name" value="UbiD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="2">
        <location>
            <begin position="124"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="4">
        <location>
            <begin position="317"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="481" mass="54574" checksum="D5C1D0524F0A19DA" modified="2011-06-28" version="1">MTFEDLRDYINFMLKRGKIVQIDDEVDVNLEIAELSRRATYSHLPPLLFTNIKNYREWKLITNIFYSIDAVKEILGVDNLEDIGKNFLTEFQGIPLSLIDKIKSLPNFLKLGKIMPKSGKPKFKEREMGLESFPALKTWPKDAGRFFTFSLVVTKDPETDVHNVSVYRIQILNDKEALMHWQAFKRGSLAAAKYKEMRINKIPVAIVNGVDPVIAFTAASPVPPGIDKYLFAGILRGEGVELYKLDNGILVPSSAESVIEGYVDLEDLRPEGPFGDHLGYYTPVDYYPTFKVDRIYIRDNPIFHATSVGKPPLEDAWIGKAVERIFLPFIRLVIPELVDMNLPEYGLFTGIGIFSIRKTFPGQAKRVMMSIWGNGQLSFLKFIIIVDADVNVHDINQVMYAIATTVDPARDVLIIPNAMNDSLDHTSLHPPLGSKIGIDATRKFKEELGREWPEEVKSDENVVKKIEPIWNKIIGKYLPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P6</accession>
    <name>F4B4P6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIB</fullName>
            <shortName evidence="5">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tfb</name>
        <name evidence="8" type="ordered locus">Ahos_1393</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776191.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P6"/>
    <dbReference type="STRING" id="933801.Ahos_1393"/>
    <dbReference type="GeneID" id="10600882"/>
    <dbReference type="KEGG" id="aho:Ahos_1393"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="5">
        <location>
            <begin position="203"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="292" mass="33472" checksum="2187D8258E58BA55" modified="2011-06-28" version="1">MKCPVCSSEDIRYDPERGQYICANCGAVLEDEVIDQGPEWRAYNYQDRLERERIGSPLTLKVHDQGLSTRIGYDKVKDRIKLMKMQKLQNRIRVSSKDKKLVTYLSMLNSEASKLDLPEHVKETAAFILRKLVENGLAKRIDSYALIAAVLYYSCQVNNIPRYLQEIKTRYSLSSSELWKALERVHKVSKQLQNFKPKIKPVEYIPKIVEKLGLPQLVATKSAEMVNIMYKNGLTSGKGYLALSAAAVYLISTLMDVKKTQKEIADSLNITEVTIRNRYKEIIKNFDIEVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U0</accession>
    <name>F4B4U0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dinitrogenase iron-molybdenum cofactor biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775022.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4U0"/>
    <dbReference type="STRING" id="933801.Ahos_0213"/>
    <dbReference type="GeneID" id="10599653"/>
    <dbReference type="KEGG" id="aho:Ahos_0213"/>
    <dbReference type="eggNOG" id="arCOG02739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044290_0_0_2"/>
    <dbReference type="OrthoDB" id="42183at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12524" checksum="ADD103D126A1B4E1" modified="2011-06-28" version="1">MKFEIFSKAKYLVLMNENGEIIEKRNNPALNSPMKRPAVAKECVELKANEVIAPHGSLCFPSYRILKKAGIKMLIANPGEDLRSSNLKEVNMKEVIYSSFLAMRERITEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U9</accession>
    <name>F4B4U9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nickel/cobalt efflux system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0222</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NiCoT transporter (TC 2.A.52) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4U9"/>
    <dbReference type="STRING" id="933801.Ahos_0222"/>
    <dbReference type="KEGG" id="aho:Ahos_0222"/>
    <dbReference type="eggNOG" id="arCOG06023">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954525_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015099">
        <property type="term" value="F:nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004688">
        <property type="entry name" value="Ni/Co_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011541">
        <property type="entry name" value="Ni/Co_transpt_high_affinity"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31611">
        <property type="entry name" value="HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31611:SF0">
        <property type="entry name" value="HIGH-AFFINITY NICKEL TRANSPORT PROTEIN NIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03824">
        <property type="entry name" value="NicO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="14530" checksum="636AC2853769B784" modified="2011-06-28" version="1">MSGFFLYIMGFLNLVVLFEIYELFKQAKLGKLDEAKLNDALLKRGFMNRYFKGLFKIVNNQYYLYPMGFLFGLGFNTASETALLAISAGTAGVFTKIPLWTLLVFSSLFTRKGLRGRKASPDGWPPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X2</accession>
    <name>F4B4X2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Succinate dehydrogenase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775054.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4X2"/>
    <dbReference type="STRING" id="933801.Ahos_0245"/>
    <dbReference type="GeneID" id="10599685"/>
    <dbReference type="KEGG" id="aho:Ahos_0245"/>
    <dbReference type="eggNOG" id="arCOG02244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150446_0_0_2"/>
    <dbReference type="OrthoDB" id="25372at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="14010" checksum="4F07D8C010D52C3B" modified="2011-06-28" version="1">MMWFSKLNIERLLALLHKITGWTILGYLIVHVIFVNRLAHGELTEPELFKYFLVLIGSVVVFHAINGIRIILIETGHLIPKHHMEEPWIYYKPHRIYIWSMIIITILSFFIGLYLVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z8</accession>
    <name>F4B4Z8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0272</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775079.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Z8"/>
    <dbReference type="STRING" id="933801.Ahos_0272"/>
    <dbReference type="GeneID" id="10599712"/>
    <dbReference type="KEGG" id="aho:Ahos_0272"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_12_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
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    <dbReference type="PANTHER" id="PTHR42770">
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    </dbReference>
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        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
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    </dbReference>
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        <property type="entry name" value="AA_transporter"/>
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    </dbReference>
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    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="132"/>
        </location>
    </feature>
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        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="242"/>
        </location>
    </feature>
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        <location>
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            <end position="287"/>
        </location>
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        <location>
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            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="11"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="424" mass="44959" checksum="E1A5D26E491716E9" modified="2011-06-28" version="1">MKRKLNLLEATAIGLGNIIGAGIFVMAGSIIYLAGPGALLSFIITGLLAMSIGLNSAELASKFPNTEGGVYSFAKLTMGDTVGFLVGWMRMISYSISGAATALGFASYLPFPSLDYVIAGVLIIVLGLIYLAGLKLASEIETTLVIINIIGLVSFISFSLLVGKFTPSHFTPIAPHGIQGILSGASLAFFAYSGFNTIATLTPDVEDGERKVPKAIILSLIITSILYILVVFSMLYVLPWQVYGTQGNPLSFALQEIHAPTYVVILISSTAVIATVTVTLSTIIATVRTLKQMAEDNLIPRRFSKDLLSSVVVLGIMISSLGLGNVEVIGLVSNFGTVFSYITTATAVIISRRRGMYGKFSAPAYPALQTISVILSLVVIISLGKESLMLGAVSLIVGLLLHEVHVQINVIEKGKRLSPHGKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B587</accession>
    <name>F4B587_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type transporter, ModC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0290</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B587"/>
    <dbReference type="STRING" id="933801.Ahos_0290"/>
    <dbReference type="KEGG" id="aho:Ahos_0290"/>
    <dbReference type="eggNOG" id="arCOG00177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="OB_MalK-like"/>
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    </dbReference>
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        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00005">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="333" mass="37381" checksum="A4AAADFAF41D2044" modified="2011-06-28" version="1">MEIEKRDFVVILGPSGEGKTTILRIIAGLLKQDKGHVYLRGKLADDMSPKDRNIAMVPQNYAVYPFMNVFDNIAFPLKIEHVPKEEIRKRVLEVAEMLKIDNLLDRKPSQLSGGQLQRVAIARALVKNADIILMDEPLSNLDAQVRVLAREELKELQQKLGPTIIYVTHDQAEALSLATKLALLHDGKIQAYGDPLELYRQPNNSWVASFLGNPAMNLIRSEIVGEKIEFNGQAIPLPEKFKGIIKSNRVILGIRPENIEISQDGDIEGVVELVEELGSFTITHVRVGNDIIKVVDKAFIKREKGEKVKLKFDYSRIELFDAESGVNLMIKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D7</accession>
    <name>F4B5D7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide/dipeptide ABC transporter, ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775147.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D7"/>
    <dbReference type="STRING" id="933801.Ahos_0340"/>
    <dbReference type="GeneID" id="10599781"/>
    <dbReference type="KEGG" id="aho:Ahos_0340"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE/OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230:SF3">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE_OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="317" mass="35593" checksum="07701C1C0D21903F" modified="2011-06-28" version="1">MEIIRLDDLTVRFTQGSFNKRIIYALRDVSLSINENEAVTILGESGAGKTTLARVLLALEKPTSGKYLFMGRNVWKDRKARKLIRKEVQYIYQDPISALNPSKTIGSSLSYPIKKHLKLKGEALRSKIFELLNAVGLDESIVDRYPSQLSGGQLQRVNIARAISVNPKVLIADEPVTMLDASYRIGIIDLLADLKKKMNMTLVMITHDLAIAKYFDYKVGGSKSFVIYGGRLVEEGYVSDIIKSPLHPYTKFLVSSYIDINGNYNSLEFLKYKEGGISSQGCPFNERCPYVKDACIKEFPRYTSIDGRKVACYLYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5F8</accession>
    <name>F4B5F8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">Ahos_1499</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776297.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5F8"/>
    <dbReference type="STRING" id="933801.Ahos_1499"/>
    <dbReference type="GeneID" id="10600999"/>
    <dbReference type="KEGG" id="aho:Ahos_1499"/>
    <dbReference type="eggNOG" id="arCOG05895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_658272_0_0_2"/>
    <dbReference type="OrthoDB" id="42118at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017007">
        <property type="entry name" value="UCP032762"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032762">
        <property type="entry name" value="UCP032762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="53292" checksum="417093A2CDEA5B6B" modified="2011-06-28" version="1">MPEGQDPLETLLKPENLEKLNKIADSLPTMEKLTDKLNEMDKKGQLDFLLNLTDQTLSLLDAVQKADLINTLISFGMEQLPKIQAIWPIIEKLTSDKTLNLLQQIDLDATLNAIEALTPMIQKLTSDKAISLLKQIDIDATLTAIEKLTPIMKALTSDKALKVLESINYDALLDSTEKLTPILQKLTSDKALKVLENLDVDTLLEATVELTPTLNRIAKTMAEMQMKGQIDELLNMMEQGVALLDAVQKADLINTLISFGMEQLPKIQAIWPIIEKLTSDRALALLQKIDVDATLSAIEALTPMMQKLTSPKAISLIQKFDVDSTLAAIEALTPMMQKMTSERAIKLVQQLDVEGLLTAMEASLPILKKLTDERTVKTLTQIDIDSMVNMMGKLAELQKNGVLDKMVRLIDVFSDPKLVDNLVLIMEKMSKALNIWVSELPNVKPVGTMGLLRMTSDKDASYALGMMKALLEATGKAFRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G6</accession>
    <name>F4B5G6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G6"/>
    <dbReference type="KEGG" id="aho:Ahos_1507"/>
    <dbReference type="eggNOG" id="arCOG10434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476065_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="79" mass="8531" checksum="6C679871F08049EF" modified="2011-06-28" version="1">MDKIFKVIFIIGLILEFVVAIIVTGNATTTPLTASQVAAVGIIAVILLVIAGVIYSQFPYSVLGKPVEKEKQKVEERSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J4</accession>
    <name>F4B5J4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoesterase PA-phosphatase related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J4"/>
    <dbReference type="STRING" id="933801.Ahos_1535"/>
    <dbReference type="KEGG" id="aho:Ahos_1535"/>
    <dbReference type="eggNOG" id="arCOG07501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093776_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21481" checksum="467D86C1F6854405" modified="2011-06-28" version="1">MMEKIISIIFHPSISTFLGFLIIFLARCRNLLDFVDIIFFFSILPFLITLVMWKLGKISDLFISRREERGRVIIIVLLGYLVGSLVLYYFNFSSIFFYLSIAYIVNTILILSISLKYKISIHVATITAISTALIMILGIKFIFFYLFSIIVGIARVKIKAHTIDQVLSAFIISIIVTFIQLKIYLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J6</accession>
    <name>F4B5J6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776335.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J6"/>
    <dbReference type="STRING" id="933801.Ahos_1537"/>
    <dbReference type="GeneID" id="10601037"/>
    <dbReference type="KEGG" id="aho:Ahos_1537"/>
    <dbReference type="eggNOG" id="arCOG05966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038122_0_0_2"/>
    <dbReference type="OrthoDB" id="42569at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042578">
        <property type="term" value="F:phosphoric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16013">
        <property type="entry name" value="AcpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007312">
        <property type="entry name" value="Phosphoesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956:SF1">
        <property type="entry name" value="NON-SPECIFIC PHOSPHOLIPASE C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956">
        <property type="entry name" value="NON-SPECIFIC PHOSPHOLIPASE C4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04185">
        <property type="entry name" value="Phosphoesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="492"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="518" mass="59424" checksum="F0CB96867FA31BF6" modified="2011-06-28" version="1">MKRTLIITFFLTIFFLLPTFHTSTTATPIKHVIIIIEENHSFDNMFGTYPFGWPPIVNNITLSVMWPCGLYKNYTQLESSKNGVLCWISVPNVPWLPFLGSSHPYYANAWDTVDPGEGWCLYHGDYWFDTYDGFVYYSGPQSMAYFSYQQVGILWDYAEEYVLADNYYSPVLGLTEPNRVAYMTGFPPSFYSDDACNVIPFNETIMYQLCENNISWGYFVYDYSGCIPWPMGAFIGIQNYMNHFHGLTAFQEYLQNNSLPAVSLVMLINGYDDRYDMHPPYNVTAGAVEISQIINEIMRSPEWNSTVIFITFDEGGGYFDQIVPPAINHYGLGQRIPLLIISPYAKEAWIDNYTLSGYTLLGFIDYNWHLPWLTKYVENSDVQGLLCAFNFSRDRPPIILCPNNWTYPIPLQYPVHYGYIAVVHPLPGYASVYPEPATQILLPLDLIGFALLFASFKKRNLTLYALLSLLVSLGISGYVYSTNSIYSFVTDYFVYSSLIGFLISSVLALKRYKNVLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5L7</accession>
    <name>F4B5L7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1558</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776356.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L7"/>
    <dbReference type="STRING" id="933801.Ahos_1558"/>
    <dbReference type="GeneID" id="10601059"/>
    <dbReference type="KEGG" id="aho:Ahos_1558"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_4_2"/>
    <dbReference type="OrthoDB" id="57516at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="251" mass="26616" checksum="30DF0837ED4BFAC5" modified="2011-06-28" version="1">MIPIWLLIIIGIAVGALTGITGSSGVLIVVPALSYLGLSFQDAIGSSLLVDVITTLSVIFVYFRHGNVDLKISLILGLGAVMGAQVGSAIAFVVPDRALESVFVVFTAYMAYVSFKRSRNPKLNIKRLNLRTASYVIAPTLAFLIGTVTGTLGASGGIMFIAVMMLLFSIDVKRMIGTATLAMLFSAISGATAYAISGRSDIIASVIIGLTALVSGYYFARLANKMRPSYIYMFLGSVFVVTSVSELFKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M4</accession>
    <name>F4B5M4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M4"/>
    <dbReference type="STRING" id="933801.Ahos_1565"/>
    <dbReference type="KEGG" id="aho:Ahos_1565"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000325">
        <property type="entry name" value="Radical SAM domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="40"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="381" mass="43036" checksum="2D77D6C73132DE4B" modified="2011-06-28" version="1">MLKIQEVFMIPVSVMVTDTGTVSFKIKGHYGKDSPSKFSEVFRPIVTWNLTYKCNLRCLHCYINASPSAPDGLSTEEALNLVDQMTEIGIPMIIMSGGEPLMRRDFFTIASYAANKGIKLSLSTNGTLISENVARKLKELGFVYVGISLDSYSPEFHDKFRGVQGAFNMAIKGIKNALNAGLNVGLRFTLTKENILDVDNYIELAVKLGVSRITFYHLSASGRGKELKDWMYTPEEYKIFMDKIMHYAKQLKGKIEIETTLGTFDGIYIAKYLNDRKLLDFVKSTGGCGRKMISIYPNGDVFPCQFIDFVKLGNIREKPLKEILKNIPDIFVNTENYLKGQKCGTCEYKEYCKGGDRSRAYYWDGSIYGDDPLCPLRELHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V8</accession>
    <name>F4B5V8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase PA-phosphatase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775239.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5V8"/>
    <dbReference type="STRING" id="933801.Ahos_0435"/>
    <dbReference type="GeneID" id="10599875"/>
    <dbReference type="KEGG" id="aho:Ahos_0435"/>
    <dbReference type="eggNOG" id="arCOG03056">
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
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        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF13">
        <property type="entry name" value="AT30094P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
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    </dbReference>
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        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="2">
        <location>
            <begin position="75"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="202" mass="23230" checksum="3C86198952974C69" modified="2011-06-28" version="1">MRKYWLFLIFFVLLSILIKIFGEDNPINVYLFELINYHQLSYLNAFMVDLSKYGRCYVWIPLNALLLIFKKTRRTGITLAASFILAIILGEASKYIMAEPRPFYFIHSNLLIPKPHDYSYPSGHALIVGDGAAVLALSSPKWLWIPLLIEALLVSYSRVYVGVHWPADILGGWLLALWIAYFTVEEERKGLLAPVEKIFKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5W5</accession>
    <name>F4B5W5_ACIHW</name>
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        <lineage>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1580"/>
    <dbReference type="eggNOG" id="arCOG09879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2379214_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10775" checksum="21B3DCE6DD556C63" modified="2011-06-28" version="1">MVLLSHSLLEYTPKFFRLTYGVYSKSLKMQKTPICVNVEEIKSIMKEQREDAENLMSRAIPRDVPKEELLTNLSIPNVLAILGEAGNLLYHSYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5X0</accession>
    <name>F4B5X0_ACIHW</name>
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        <submittedName>
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    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1586</name>
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        <dbReference type="NCBI Taxonomy" id="933801"/>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    <dbReference type="AlphaFoldDB" id="F4B5X0"/>
    <dbReference type="GeneID" id="10601087"/>
    <dbReference type="KEGG" id="aho:Ahos_1586"/>
    <dbReference type="eggNOG" id="arCOG03706">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="14692" checksum="FD43C55CE37F628C" modified="2011-06-28" version="1">MKYYIDTSLAIALANEKDPNHEMAIKIYPKDGKKVISKLVLAELYSVYSRRMKVSDEELEALVNYTLNKCKCEIEEVDFDKVIDFAVKISNKVKLKTLDLLHLSISIILESEILSLDDEIIKAKNKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Y7</accession>
    <name>F4B5Y7_ACIHW</name>
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        <recommendedName>
            <fullName evidence="3">Methyltransferase FkbM domain-containing protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">Ahos_1607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776400.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Y7"/>
    <dbReference type="STRING" id="933801.Ahos_1607"/>
    <dbReference type="GeneID" id="80457670"/>
    <dbReference type="KEGG" id="aho:Ahos_1607"/>
    <dbReference type="eggNOG" id="arCOG01401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447482_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="89" mass="10424" checksum="DEA1FADDC4424781" modified="2011-06-28" version="1">MDIEGSEGKALIGGINTLKHLELIEIEVHDEENMEKVEEILSRFRKRELAIEDLGNVYKQFLEHPFEILRIEMANRFSTTKRVLLSKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B605</accession>
    <name>F4B605_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1625</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776418.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B605"/>
    <dbReference type="STRING" id="933801.Ahos_1625"/>
    <dbReference type="GeneID" id="10601125"/>
    <dbReference type="KEGG" id="aho:Ahos_1625"/>
    <dbReference type="eggNOG" id="arCOG03663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_7_2_2"/>
    <dbReference type="OrthoDB" id="27033at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06604">
        <property type="entry name" value="GH31_glucosidase_II_MalA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053497">
        <property type="entry name" value="GH31_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040948">
        <property type="entry name" value="alpha_gluc_MalA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF120">
        <property type="entry name" value="HETEROGLYCAN GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="6">
        <location>
            <begin position="51"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="168"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="7">
        <location>
            <begin position="524"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="679" mass="78813" checksum="30596C1537166FD4" modified="2011-06-28" version="1">MKVVIKESGEGIYKIIVNDPFPPVEFPFEGKDSEKDLSFFSLNITERDGNILIEKDLDIKEHVLGLGEKAYELDRRRGRFIMYNVDAGAYGKFDEPLYLNVPFMITVKNGIAKGYFINSASKVIFDIGVEDYGKIKIKIPEPSVEIYYFEGPTIEKVLEQYSEITGKPFLPPIWAFGYMISRYSYFPQDKIVKLLDELKEEGFNVTGVFLDIDYMDSFKLFTWNKERFPDPRKFIDEVHSRGVKVITIVDHSVRVDQNYEVFISGLGKYCETDKGDLFVGKLWPGNSVYPDFFREETRDWWSELISKWLSQGVDGIWLDMNEPTDFTKVFQIREVLGSTPIELRDDRIYYTFPDNVIHYLGGKKVPHSQVRNAYPYYEAMATFEGFKKVGKDEIFILSRSGYAGIQKYAFVWTGDNTSARDQLILQLQTVLGLSISGIPYVGIDIGGFQGRLSKVENSPEILLYMFRLAMFFPFFRTHKAKDGIDVEPIYLPYYYKEKVRDVINTRYKFLPYIYCLAKEAHETGHPIIRPLFYEFSEDEDTYNIEDEYMLGKYVLYAPQLGENRVVYLPGKWFEYSTGEERSGWVKTNADLPIYIRENSVVNLDRNDLIVLGNSKLKCEIEIEKKGNIITFSKPYFVNILVTDKAEKVIIDDNEVKTEDFGALSKVKINKEVRKVELVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B612</accession>
    <name>F4B612_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776425.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B612"/>
    <dbReference type="STRING" id="933801.Ahos_1632"/>
    <dbReference type="GeneID" id="10601132"/>
    <dbReference type="KEGG" id="aho:Ahos_1632"/>
    <dbReference type="eggNOG" id="arCOG06035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867723_0_0_2"/>
    <dbReference type="OrthoDB" id="44205at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="313" mass="35602" checksum="0F28A3E38CB6EBB2" modified="2011-06-28" version="1">MKNVIEPWGVNVPFLILAFIYFAIGGVSLNLDPSVHGFFMFLGAYSLYSGMILRLFFPAKKYLILHIVTLVLLSIPLYPFVSLSFFFLTIVEIWGLKDVKSYGSKFPINILVLSSPPLSFISWLLFPILGYNLLIISLLLYLLGINEGIFSATLGLKPKFGIKQIPILLLVFLFYLSYSLFPLIIIAYFAWLFYGVKKVRKNLSALSVILSSASTSIGSYFLGETIHAFALGTMAPFFYSCITYSTSRYNYDKIYIISILLSLSYFLRFVYFHISGIFFVASSLLFLYLIKDNLTLTTLKYGMSKKYLIKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B652</accession>
    <name>F4B652_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable thymidylate kinase</fullName>
            <ecNumber evidence="7">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tmk</name>
        <name evidence="9" type="ordered locus">Ahos_0452</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 7">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the thymidylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775256.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B652"/>
    <dbReference type="STRING" id="933801.Ahos_0452"/>
    <dbReference type="GeneID" id="10599892"/>
    <dbReference type="KEGG" id="aho:Ahos_0452"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="205" mass="23146" checksum="4DAC6E43F7D9A6DC" modified="2011-06-28" version="1">MIIAFEGIEGSGRTAHLDAVKRYLEKEGYGVVTFGIQMSKLIGERIAQVKKEIVFERRTLFLAYVTDLADQIENVVKPAIDSGFIALADGYVLTLMAYGLARGLEKEWMTEVLSIFPKPSLSFSLVSTPEEIMKRIIKKRGYLDPLSAGIDICVKEDVFSAYNEYIERFQKYLVELSINSKVINTARDFSEVNKEIVSEIEKLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B660</accession>
    <name>F4B660_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glutamine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0460</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
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        <cofactor evidence="1">
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        </cofactor>
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        <text evidence="6 7">Belongs to the glutamine synthetase family.</text>
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        </source>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="429" mass="48734" checksum="EE4C04485C3134D3" modified="2011-06-28" version="1">MQVGQELKEKGIDILRFTWVGLDGYIRAKGAYVDHVDEMVKTGIGLTKAMFSFTPMDTLSPYGSFGPEDEDVFLVPDLSTLSIFPPSAMVICNLYSNGKPWEYDVRSTLKSTLDKIREEYGYEFKSSFEIEFYLVKDKKPYDDARCFDPSAFYNNPVVSEIAKLLDDNGIDILRVIKEYGPGQYEFDILHKESLRSADEVVIFKEIARQVATKHGIEANFMPKPFNKLAGSGLHLNLSIWKGNSNLFFDDKDKLGLSEIAYSFIAGIIEHAKALTAIAAPTINSYKRLVPGSWAPTKIAYGYNNKSSMLRIPTPYPGMSSIDRRIEYRVPDPSTNPYLLLTAIIQAGMDGIERGLKPPSPVNENAYYRKDIEDLPRNLREALNELNKDSTLKEKIGNKIIDEFIKVKTAEVEEYESYVTDWEYEVYRKL</sequence>
</entry>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="GeneID" id="10599935"/>
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    </dbReference>
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        <location>
            <begin position="9"/>
            <end position="29"/>
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        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11275" checksum="6DA04A67B9054A93" modified="2011-06-28" version="1">MISQYLEELGILLIMLGFLIVFLGILYEASKPREEGEEKETQAGGIILIGPIPIIFGSSKKIEKWMLIIALVIVVIMALLYLYEASGLHGINYSLYSNYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A6</accession>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="182" mass="20507" checksum="021D0C8136648BCD" modified="2011-06-28" version="1">MVIVGLVFAIIIAFFVVLMMPVEIYPQNNKSLGNISMLEIHNELDLNYSKANITYYAPVIPSNFTIYGQLLVYSLLDMKAGPMGTNNISLFILSPSYICTNSGNSRITFNISITLISNNNKIHVTIPSCEKYYFICSIYENMANNKILLSISIKLNNLQKDSLLIIPIKVNSCTQYLQIYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D8</accession>
    <name>F4B6D8_ACIHW</name>
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        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
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    </protein>
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    <organism evidence="6 7">
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D8"/>
    <dbReference type="STRING" id="933801.Ahos_1681"/>
    <dbReference type="KEGG" id="aho:Ahos_1681"/>
    <dbReference type="eggNOG" id="arCOG05825">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067819_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
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        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
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        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF2">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="5">
        <location>
            <begin position="5"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="27172" checksum="6A591FA5898659DB" modified="2011-06-28" version="1">MADGSTREKFEKEISTAKGGVIISGGSTPRGDVPVWKYSDILRNRKDLTIIAHTGVVKNEEIARKFKESGVKIALLDMVGDNETIKEVLGQPFTVDDYLNSFKYLKKEGIKIVPHVIMGLSKKGEEGDLNAIRLLKEVNPDAVIIVGLMPLVGTAYNEISPPSPSHLINGLKLARDLFNVPVMLGCARPLGSKYSEVEKFAVDYEVDGIAFPEENTIEYAEEKGRKVILSNACCSNVVYDFILRVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E5</accession>
    <name>F4B6E5_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1688</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776480.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E5"/>
    <dbReference type="STRING" id="933801.Ahos_1688"/>
    <dbReference type="GeneID" id="10601188"/>
    <dbReference type="KEGG" id="aho:Ahos_1688"/>
    <dbReference type="eggNOG" id="arCOG05920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754796_0_0_2"/>
    <dbReference type="OrthoDB" id="33156at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="148" mass="17029" checksum="BC3B65C44ACA488C" modified="2011-06-28" version="1">MKEKLGKIEVEVKGEIEINGETYKIAEVPSADEYKGFPPSWEFVKNSMLSWKPYFKGKMVEINGQLIPAVGNYLLNMDEEMYELTLRVYQAFKLNKPLIETNISVVVTDQINEVERKIGRALSSEEKTAYYIRYAVELAILRDIGLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H3</accession>
    <name>F4B6H3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1641 domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1716</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776508.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H3"/>
    <dbReference type="STRING" id="933801.Ahos_1716"/>
    <dbReference type="GeneID" id="10601216"/>
    <dbReference type="KEGG" id="aho:Ahos_1716"/>
    <dbReference type="eggNOG" id="arCOG02113">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067846_0_0_2"/>
    <dbReference type="OrthoDB" id="36283at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433:SF1">
        <property type="entry name" value="DUF1641 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13317" checksum="B51A5BB63FF08976" modified="2011-06-28" version="1">MDERGIQAIDGLVEQIVESKDALEQFLRLLNALQKTGILPLLVGIVEKLDENLAFLAEQNAMLIRNVNMIYSVLSGKEEINNVSLGELIMELKDPDVKRGLYLVLKILKAIGSANKESKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K2</accession>
    <name>F4B6K2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0524</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K2"/>
    <dbReference type="STRING" id="933801.Ahos_0524"/>
    <dbReference type="KEGG" id="aho:Ahos_0524"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_6_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000826">
        <property type="entry name" value="Replicative DNA helicase Mcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048907">
        <property type="entry name" value="WHD_MCM_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040949">
        <property type="entry name" value="minchrom_main_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21100">
        <property type="entry name" value="WHD_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="10">
        <location>
            <begin position="256"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="652" mass="73281" checksum="16A1E36F44ACDE1D" modified="2011-06-28" version="1">MIAYRKKSLLLDFSDIYSYNDKIATEIIANPLYTLKILDEKLLKIISEIDPTYPDEVERVHVRLINLPRTIELRKIRSNYINKLITVEGILTKQTPVKERAYKVVFKHVHPDCNQEFEWPEGDEEMDEIIKTPTVCPLCGKPGQFEIVAEKTKLTDWQKVILQERPEEVPPGQLPRQLEVVLEDDLVDSARPGDRVKITGILLIKQDSIVKRGSRAVFDVYMKALSIEVSQKVLDEVEITDEDKKKIEDLAKDPWIKQKIISSIAPSIFDHWEIKEAIALALFGGVPRVMPDGTRIRGDIHVLIIGDPGTAKSQILQFAARVAPRSVYTTGKGATAAGLTAAVVREKNSGDYYLEAGALVLADGGIAVIDEIDKMREEDRVAIHEAMEQQTVSIAKAGIVAKLNARATIIAAGNPKFGRYIAERGISENIDLPPTILSRFDLIFILVDKPSDEDQRLATHILDMHGGKPVKDIIPVDLLKKYIAYARKYVNPELTEEAKQLLADFYVEMRKKSSESPDSPILITPRQLEALIRLSEAYARMALRKTVTKEDAENAINIMRIFLEKVGIDVESGAMDIDTIMTGKPKSAREKMVKIMEIIDTLSGSEGCAKLREIVKEAEREGIERQSTEKLISDMKKSGLIYETKPECYKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L6</accession>
    <name>F4B6L6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L6"/>
    <dbReference type="STRING" id="933801.Ahos_0538"/>
    <dbReference type="KEGG" id="aho:Ahos_0538"/>
    <dbReference type="eggNOG" id="arCOG07178">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736315_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="150" mass="17331" checksum="CF99362A78A817B3" modified="2011-06-28" version="1">MYFMGKLVFIKDGRIIFNNERKLEDCVELPFLVEENYLKFKDLSIPLIFSDERRKLARLFLLLSLSTSHEVFNCCENVKIFIDSKLAEVNLNNLKRGFTKICGNYGSTKLVYCISNESIAIMGRSEKDSQKALDEIKEFVSLLSSINNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S8</accession>
    <name>F4B6S8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CRISPR-associated protein, Cas5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1744</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-A/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776536.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S8"/>
    <dbReference type="STRING" id="933801.Ahos_1744"/>
    <dbReference type="GeneID" id="10601244"/>
    <dbReference type="KEGG" id="aho:Ahos_1744"/>
    <dbReference type="eggNOG" id="arCOG02670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154391_0_0_2"/>
    <dbReference type="OrthoDB" id="99243at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09753">
        <property type="entry name" value="Cas5_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010153">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053725">
        <property type="entry name" value="CRISPR_Cas5_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01874">
        <property type="entry name" value="cas_cas5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="240" mass="27298" checksum="8F162631180D946C" modified="2011-06-28" version="1">MIYSKVFLKLHWGFSVSVPSVSKSRPSVYLPPPTTLIGALSYGKYRGYDTVLVNRRPGSPAYNLLNVKAAASFSKDFRGTYIEDIVRNVILYFQRSGRKTDPKYRYGVIPSGKVYSPNGKLNVVYVTDSIDRSELERLSWSITRIGCKECLVSVEDVELGEAKKVSGKVRTRYYFPDNVKIIDGKVEYVEFWNEKGFLWGSESDRIRYALPIITYPLMSVEVEVEAKEAYEVGGEYVVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T4</accession>
    <name>F4B6T4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CRISPR-associated protein, Cas2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T4"/>
    <dbReference type="STRING" id="933801.Ahos_1750"/>
    <dbReference type="KEGG" id="aho:Ahos_1750"/>
    <dbReference type="eggNOG" id="arCOG04194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3263579_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405:SF3">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143430">
        <property type="entry name" value="TTP0101/SSO1404-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="41" mass="4746" checksum="1F5C483ABDBF1911" modified="2011-06-28" version="1">MKIIVVYDISDNGKRNKLANELKKFGLYRIQRSAFEGDIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U4</accession>
    <name>F4B6U4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776552.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U4"/>
    <dbReference type="STRING" id="933801.Ahos_1760"/>
    <dbReference type="GeneID" id="10601260"/>
    <dbReference type="KEGG" id="aho:Ahos_1760"/>
    <dbReference type="HOGENOM" id="CLU_688112_0_0_2"/>
    <dbReference type="OrthoDB" id="385944at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="400" mass="47366" checksum="F9CA7B8C4BD2F27D" modified="2011-06-28" version="1">MNLLTVKKYLFKQSLTKFQEIQLKYINSILQDILVPILCDYKNIDIIKSQITDIESFYFGFKQNPSSISEKLALPLYISNRFFIASSGPRFGSIYEDLIRSFLENFGYQVDERVNIFKYTLFSEYYQKKYSKQQKVIDFIARKDNKLYLIEQRTSEHSGGRTGQESLLDKFKVFLNWIIEKSADPLINKGLREIYLIIFISYSQKHEILTEQNVNRGRINSLIEYIVENLGDYFTRLTNKGFKTECIDLASCLEKYRRLIFYTNQIKIEFRIMLGEEFYKEILGEKYSSLKNKILNEELGDDLWVIYSILPYELRLYYEEGFMWSNRIYEMLISRYNNIITSASNEDDIINKIASKIIEDQKDLRLLETNNLSKQYEYLKTLCAASLILYALKCPSRLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X2</accession>
    <name>F4B6X2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776580.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X2"/>
    <dbReference type="STRING" id="933801.Ahos_1789"/>
    <dbReference type="GeneID" id="10601289"/>
    <dbReference type="KEGG" id="aho:Ahos_1789"/>
    <dbReference type="eggNOG" id="arCOG01075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_9_3_2"/>
    <dbReference type="OrthoDB" id="40462at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04683">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43046:SF16">
        <property type="entry name" value="ADP-RIBOSE PYROPHOSPHATASE YJHB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43046">
        <property type="entry name" value="GDP-MANNOSE MANNOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="143" mass="16715" checksum="2129D8AFD6671B42" modified="2011-06-28" version="1">MNRYPQILSVHLFLLEGDKILLQLRKNTGYRDGCWSVIAGHVEAKESAKRAMIREAKEEAGILLNPEDLILVHVMHRFENQERVDFFFKAKRWEGEPKVMEPNKAEKVEWFKLSELPPNTVPYVKQAIELGLMKGIIYSEYGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B714</accession>
    <name>F4B714_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein Ahos_0609</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775413.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B714"/>
    <dbReference type="STRING" id="933801.Ahos_0609"/>
    <dbReference type="GeneID" id="10600056"/>
    <dbReference type="KEGG" id="aho:Ahos_0609"/>
    <dbReference type="eggNOG" id="arCOG01336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_2"/>
    <dbReference type="OrthoDB" id="25187at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="12"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="221" mass="24764" checksum="0852582E06BC604B" modified="2011-06-28" version="1">MSLVTIQELGIEEGKTLVKIARQAIMKKLGLQSSISYKESEVLNKKGLAFVTLETLLGETTTLRGCIGYVEAVAPLKEIVKNAAIAAAFSDPRFPPLMKDEINNIIIEVTVLTKPEEVVVDDRKELPKVIKVGRDGLIVEKGILYSGLLLPQVPMEYCWDEETFLAETCLKAGLSPDCWLDKNVRIKRFEGIIFREKYPNSDEILMIKPSDVKCKPFDFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B715</accession>
    <name>F4B715_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutaredoxin-like domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775414.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B715"/>
    <dbReference type="STRING" id="933801.Ahos_0610"/>
    <dbReference type="GeneID" id="10600057"/>
    <dbReference type="KEGG" id="aho:Ahos_0610"/>
    <dbReference type="eggNOG" id="arCOG01218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082677_0_0_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02973">
        <property type="entry name" value="TRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011903">
        <property type="entry name" value="TON_0319-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02187">
        <property type="entry name" value="PDO_seleno_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170">
        <property type="entry name" value="GLUTAREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="149"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="236" mass="26386" checksum="F9E13D47587ACABC" modified="2011-06-28" version="1">MSEEEYAELFTDEVRDALKDALKDMKNPVDVYVFIDSKDTNCNYCGLTKKLLEFISEAAPQKDGKSLLQVHVEDRGSGDPEVEKVFKEFKVERVPTVAFCKGYIRWTGAPVGEEIRALVETIVRLSQGESGLSQETIQAIKDKLNGEVKVEVVVTPSCPYCPYAALLSHMVAYEACKANKCDILSDVVEAYENQDIADKYQVMSVPTLAINESVEFIGVPNEENFVNTILEKQKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B719</accession>
    <name>F4B719_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Exonuclease, beta-lactamase fold, RNA processing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775418.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B719"/>
    <dbReference type="STRING" id="933801.Ahos_0614"/>
    <dbReference type="GeneID" id="10600061"/>
    <dbReference type="KEGG" id="aho:Ahos_0614"/>
    <dbReference type="eggNOG" id="arCOG00545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050517_0_0_2"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF35">
        <property type="entry name" value="DNA REPAIR METALLO-BETA-LACTAMASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="329" mass="37079" checksum="C2B1907C000ADAAA" modified="2011-06-28" version="1">MFFDIKRSGAILLGNNFTVDGHYERNFRVVTHFHADHILQLNKSISTCIGIISTPPTLEVLSVLGYEIPKKKAMGISYGVKIAIEDEKIELVQADHVFGAAQVVVTNSNGESVGYTGDFKNPGKGTPILNTDVLIVDTTYGKPGFRRKFRDEVEILFSDYVNDSLIYGPVRVYAYYGKIQEAMKILRRNGISAPFIVDGKIKEVTDIAIKYGLEIKDVFSAKSPEAKDIIKDGWYVEFKHFHEFKNRDSKFTNFALSGWEFSSPIRDVDNKSKIVALSDHADFDELVYYVDSSSASLIVTDGGRKGYYKEFAEYVNRYLNKKAISLPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B732</accession>
    <name>F4B732_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AAA family ATPase, CDC48 subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0627</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775431.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B732"/>
    <dbReference type="STRING" id="933801.Ahos_0627"/>
    <dbReference type="GeneID" id="10600074"/>
    <dbReference type="KEGG" id="aho:Ahos_0627"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000008">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000178">
        <property type="entry name" value="CDC48/VCP homolog, AAA superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="118"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="223"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="496"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="767" mass="85469" checksum="D7E121925D0104DC" modified="2011-06-28" version="1">MAASSSAEEPRKRELILKVVEARQKDVGRGKVRIDLDLLSQIDVSPGDVVEIEGQRKTAAIAWPLSADDYTGEKDIIRMDGITRKNAGVSIGDKVIVRKATVKPATSVKLAPSNFSITVDPGFVAYVKKKLKEFPLVEGDTVLIPVLGQAIPFTVIQVRPAGIVMVTDETSINISDKPVEQTRYPRVTYEDIGGLKNIIQKVRELVELPLRHPELFKRLGIEPPKGVLLYGPPGTGKTLLAKAVANETDAYFTSINGPEIMSKFYGESEQRLREIFEDAKKHAPAIIFIDEIDAIAPKRDEVIGEVERRVVAQLLTLMDGLESRGNVIVIAATNRPNAVDPALRRPGRFDREIEIPLPDKQGRLEILQIHTRNMPLAKDVDLEKLAEVTHGYTGADLAALVREAAMNALRRYLPKIDITLDKIPPEILESMEVKMEDFMNALKEIVPSGMREIYIEVPEVRWDDIGGLGDIKEELREVAEYPLKFQEYYEMTGIEPPKGILLFGPPGTGKTMLAKAVATESGANFIAVRGPEVLSKWVGESERAIREIFRKARMYAPTVIFFDEIDAIAPMRGMSPDTGVTERIVNQLLAEMDGIEKLDNVVIIAATNRPDILDPALLRPGRFEKLIYVPPPDKQARYEILRVHTKKVVLGEDVNLEEIAEKTDGYTGADLAALVREAAMIAIREGMKTCIDKVSNLCPPTDTDCRDAKMKECMKGSSVKIEMRHFEEALKKVKPSVSQDMIQFYQSWLEKARQQLPRQVLKPSTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B755</accession>
    <name>F4B755_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Farnesyltranstransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0651</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775454.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B755"/>
    <dbReference type="STRING" id="933801.Ahos_0651"/>
    <dbReference type="GeneID" id="10600102"/>
    <dbReference type="KEGG" id="aho:Ahos_0651"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053504">
        <property type="entry name" value="GGPP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041003">
        <property type="entry name" value="GGPP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF85">
        <property type="entry name" value="SHORT CHAIN ISOPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="326" mass="36509" checksum="FEA28D8DF5439F71" modified="2011-06-28" version="1">MELDKYFDEIIKNVNEEIEKYIKGEPKELYDASIYLLKAGGKRLRPLITVASSDLFSGDRKRAYKAAAAVEILHNFTLIHDDIMDEDTLRRGMPTVHVKWGVPMAILAGDLLHAKAFEVLSEALEGLDSKRFYMGLSEFSKSVIIIAEGQAMDMEFENRQDVTEEEYLEMIKKKTAQLFSCSAFLGGLVSGAEDKDLELLKEFGLNLGIAFQIIDDILGLTADEKELGKPVYSDIREGKKTILVIKALSLASEAERKIIIEGLGSKDQEKIIKAAEVVKSLSLNYAYEVAEEYYQKSMKALSAIGDNNIAGKALKYLAEFTIKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B765</accession>
    <name>F4B765_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="9">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rpo3</name>
        <name evidence="9" type="synonym">rpoD</name>
        <name evidence="11" type="ordered locus">Ahos_0661</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="9">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775464.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B765"/>
    <dbReference type="STRING" id="933801.Ahos_0661"/>
    <dbReference type="GeneID" id="10600112"/>
    <dbReference type="KEGG" id="aho:Ahos_0661"/>
    <dbReference type="eggNOG" id="arCOG04241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_3_1_2"/>
    <dbReference type="OrthoDB" id="84933at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07030">
        <property type="entry name" value="RNAP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="10">
        <location>
            <begin position="11"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="29168" checksum="E37C699BD8D4F005" modified="2011-06-28" version="1">MIDVIKKEGNFISLSLDGYPLEFVNAIRRACMLYVPVMAVDDVYIIENNSPLYDEILAHRLGLIPFSSDEAIDHYRPSEECANCQENCDGCFSKIYLDVEAKDSEVMVYSKDIKSDDPAVVPISKDIPIVLLGKNQKVSLEARLRLGYGIEHAKFNPVTIAVLRYYPHIELKDDSCVKAAEVCPEKVFAVKDGKLVIANEIACTLCEECLRYCEGIKISSVENKYILEIESVGSLKPERILLEAGKSIVRKLEELKKVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B790</accession>
    <name>F4B790_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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    <sequence length="334" mass="38625" checksum="BD3BAB04FC5DA480" modified="2011-06-28" version="1">MAFLQQVISTASGLSSVVSTVALVWTIYYYRKQTELLKTQIDEMKKESSIQECLQLRDSTIRLHEVLSPLMLDEIGEIENFRDELNKVCSSPSVSCISYIHNLFSRPEEIGEFKEKISRILRDLNSLKVNDPRFKNYEEFVKDVYSSICNIPYSSSKGECELLEEIERMKNEYSEIIKLTDVPLINDIKGNMMKEIELGKEVGKMGIDDLFDVVDKFSFIIDGKCGDKTCKDVFPLRDVIEGKINPLKEMIENPKEFCKKYGLELGCDEYKPLLVKIRDVVEDDEESKVFYSILYLNWISNQFKTMVNTIQLPSEIDIKVRMKAELERITCISS</sequence>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </dbReference>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="135" mass="15492" checksum="01173FC070BD2FB3" modified="2011-06-28" version="1">MTKSVSKLKIEGKDVIMIELRKHGIDSIMLNGEIKVGEYDGVDFVKKEVSEEKMKIAKEYSLKVKELLNLCPCIISIVYSDMLYVKFYYDSTDVIAFISQNGYTTYNKQISIDKSTEERIKDCALKFLEILGVKL</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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            <dbReference type="PubMed" id="21607549"/>
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            <strain evidence="2 3">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="STRING" id="933801.Ahos_0722"/>
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    <dbReference type="KEGG" id="aho:Ahos_0722"/>
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    </dbReference>
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        <property type="entry name" value="UCP014484_TM"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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        </location>
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        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93609.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="252" mass="30162" checksum="C9FDEE4FB66E18B2" modified="2011-06-28" version="1">MSASQYNPYNWKFLLFTQAWLIIISIVLTYYPQYYFEVIILYIIVIFGFTFFMTYKSNPVFRDRKLLYEIVNSRTIYEEKDVTKLIMADQEYQKQYMDSIKRNFRMLGFYIVYLAILFFAYQYIIKFADSQTLTYRLLVYLVYFEALFGIGFFVSRKILAPSMSNMAPMALPSYKITEKGIVSGKGYSVFLHAKYLLNSEITINREKHYIEIDSTKQKLPYKVRLYSQDIDKVLDYIERVKRLELKRQSSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R1</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="340" mass="37528" checksum="834618F7A59C257E" modified="2011-06-28" version="1">MNEFVLFFFIPLAWFVTAFMIEAGSVVMIPVCRYENYREKMLIVIGSLWAIIATSLVYLVVSLDTIFAPVMFVTGAVLYAPLMVLVIMLAFHHFSIGVAEGSSTLGKHSMERTFLVLALPFALIVAFLGNTLFTSVFSGYGFCVHNLLTLDITPNYTQMIFNAFNWVFFIGVVMYVVYFTVIFYGIKERLLVGALGLALANILFLVSTYKWLPVVFNSAVNNAGFWIYIVILYAYLYIATTRNVPFRQAWIFLLTFIGAMMFGAFTQGRILEDIVPAGTVGPLPGVPASLLLTNATTLTAGSIVLGMAGLLVLGGITAVSYKVLYKVATERVETKKATVK</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </reference>
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    </evidence>
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    </evidence>
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    <sequence length="102" mass="11973" checksum="BE61C7B8B2A90F7E" modified="2011-06-28" version="1">MPTKARIRLWSTNVKDLNYVVTQIKAIVDKTGISMRGPVPLPTKRLEVPVMKLPHGEGRKKWEKWEMSIHKRVIDIAADERVMRQLMRVRVPEDVYIEIELI</sequence>
</entry>
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    <accession>F4B842</accession>
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        </source>
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <source>
            <dbReference type="EMBL" id="AEE94866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="216" mass="25788" checksum="B448A9C6EF38DE64" modified="2011-06-28" version="1">MRKLTVEEYFNSYLPKPNEEETYLFYLISRCREAKEQGLKIDKVLYRIWDVEPLKAVKILSAIRDNVEFEVRGIKVKKEWIKIMHVLNPVNISKASKEAVIRYMQECERKPDISIILFSELPRHIDFKIFMIDMDSKDPKVLEQMKRLKPRLVITTRRGFHIHFWKEDLEDPQLLFKLKDVEVKTRDSIEYVPISQGSFTPEAFEINDVEEIINLI</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    <reference key="2">
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="GeneID" id="10601516"/>
    <dbReference type="KEGG" id="aho:Ahos_2017"/>
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        <source>
            <dbReference type="EMBL" id="AEE94889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="702" mass="80720" checksum="B12F440B116ECDA2" modified="2011-06-28" version="1">MYKISNVTGDGSIINLTLPTSLRGHNEPLSVEVNLELFNPLKDERKYILLVRDVTSNVIHLAIVFSQKDKKVISISSSDFILGKLYRKREVEFIIPWVEELIAQEGRSFDFSIDIYSASREVELSKLSEIVRKAIVNSEDLQNLRFTLCRKIKFTLSWGKIGKRKLLSYSLPSIFNDFSKVEITSFANVAGEVEMYECERNKAIFTCRKIKNRINSLNVPKEISSLKLEEGKLYAFFSLSTPKIVETKGYDYFTSISFPIHFYNAPILIIGLNGLNKSEKEGAFANVIYPMPKKQRVTLPKFLRNNSWVSSLPKYIGEEKGDEEISDLTPTFNRKRILERILNKFYSSTITPVNGIKEMELNEGKAFSGDFNFKIIPANDKILRREFRKFINGLGKILYLSASSGRFSENPRKPSFTLKYSVIEKLSKKYNFEVNNGIMSGNIPHMLVPLLIKLYAGAEVKDKFHDIFKGYAGKIKERVDLILQEISSLSSLPPTNLETIIKYYSDSDELYEEIKRVLESSNSMTQAYYILLASLLFLTSRIKDDRKRKNVESDEITNLMGSNHVLRLSNVEAYLINSFSPYSRVYIPVLNSSFVRVRINTMNSKFAFLIDTAVEKLFSVVTYNLKLLLTPKGENSEVIIDHPEHTTFRIKIEKPIILSIEPKPIVQVINTDAIFFDFDVSKVFFVSQYLWERHISRDFEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B883</accession>
    <name>F4B883_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4-hydroxybutyryl-CoA dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776822.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B883"/>
    <dbReference type="STRING" id="933801.Ahos_2036"/>
    <dbReference type="GeneID" id="10601536"/>
    <dbReference type="KEGG" id="aho:Ahos_2036"/>
    <dbReference type="eggNOG" id="arCOG02143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023920_1_0_2"/>
    <dbReference type="OrthoDB" id="32865at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3140.10">
        <property type="entry name" value="4-hydroxybutyryl-coa dehydratase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004925">
        <property type="entry name" value="HpaB/PvcC/4-BUDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024719">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024674">
        <property type="entry name" value="HpaB/PvcC/4-BUDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36117:SF3">
        <property type="entry name" value="4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03241">
        <property type="entry name" value="HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11794">
        <property type="entry name" value="HpaB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000331">
        <property type="entry name" value="HpaA_HpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpaB/PvcC/4-BUDH N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="HpaB/PvcC/4-BUDH C-terminal" evidence="5">
        <location>
            <begin position="282"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="491"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="514" mass="57273" checksum="9AAC16C0E6A95F2B" modified="2011-06-28" version="1">MGIRTGQEYLDSIKVRNKVEIYVMGKEVKDVTTNPFLKPSVLAFKATFDSAWDEDTKDLARVWSPFINEEVNRFNHIHRSPEDLAAKVKLLRKISHKVGACFQRCVGYDALNTLYIITELMAQHGKTEPKEKFIEYLKTVQKKDLALAGAMTDAKGVRTLKPHEQPNKDAYVRITEVTKDGIYVSGAKANITGVAASEEIVVLPTRAMGPEDQDYAVAFSIPTDTEGIKIIVGRQLNDARRLEGGEIDALPYFYNHEGLIIFDNVFVPMERVFLMRDWQYTSQLVEIFSAYHRQGYGGCKAGLGDVIIGATYNLAKQIGVEKAPHVQDKINEEIFLTETMYAAGIAASLNGIEVCPGCWWVNPMQANVTKHLVTRFPSQIAQLAIDIAGGILGTAPSEWDLKNPKIKELLAKYLQGVEGYTAEDRIRMVRLLENVALGVAFQIESVHGAGSPAAQRIMFSRLYNLSYAEEVAKRLAGMKSNVKFTKKVEPWKESETEKLAKEAEKNTETASGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B891</accession>
    <name>F4B891_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776830.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B891"/>
    <dbReference type="STRING" id="933801.Ahos_2044"/>
    <dbReference type="GeneID" id="10601544"/>
    <dbReference type="KEGG" id="aho:Ahos_2044"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_757797_0_0_2"/>
    <dbReference type="OrthoDB" id="385555at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="41015" checksum="FF4881D1BAB95B79" modified="2011-06-28" version="1">MKRIALTALISRIANNIILTLSPVIAKIDYHFPLGSLGIIEAIVLVSIIISMLSINLIYSREILLLSFILLSVSLILLNMHNVSIFFLSLILAGTSFGVIIPSMTTIASSIADDYHSNARLLKMYTFGLSIGAVISALLEAISLGNPIFLLMAISALNLYNAYFYYLNFRNKAKKSMISFRIIFNKRLWIANMISLPYDIPIAFINVYLALLAHYYYHVSISLGFLYFFVFLLVASLSRYLIYRLELEESKMRIFSAVMTILGSILLMVNNPFFLLFGIIFLGFPHGLLLTLSSLVIAREFKDEKDKNLANSINLLFHYAVFIITPLIIGLFINISIFYGILAFVVLSIGSSLLLMSMSKNNYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B895</accession>
    <name>F4B895_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776834.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B895"/>
    <dbReference type="GeneID" id="10601548"/>
    <dbReference type="KEGG" id="aho:Ahos_2048"/>
    <dbReference type="eggNOG" id="arCOG07844">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140800_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="13031" checksum="AB5536B8D3A96304" modified="2011-06-28" version="1">MLNKCLVALIPLIATLWIFEWTRKIAMVVIDLLIGLMVAGLMAAITFAILATTPLGAYLFIIDPIAMDGEFLFSLTFFVFGLGPGEHMMGAFRKKNGGGSGNTVVVENGGSTSSEPPAGRYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A9</accession>
    <name>F4B8A9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Nucleotidyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_0822</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775624.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A9"/>
    <dbReference type="STRING" id="933801.Ahos_0822"/>
    <dbReference type="GeneID" id="10600289"/>
    <dbReference type="KEGG" id="aho:Ahos_0822"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05636">
        <property type="entry name" value="LbH_G1P_TT_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053650">
        <property type="entry name" value="Sugar-1P_NT/AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041173">
        <property type="entry name" value="Sug_nt_acttase_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="11">
        <location>
            <begin position="3"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="12">
        <location>
            <begin position="263"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="405" mass="44736" checksum="D5605E486FF18920" modified="2011-06-28" version="1">MQVVILAAGKGERLEPITHTRPKPFVPLLGSTLIERTINSIKERNIGNEILVVIPSDYPEEYKEFYSRLKDVKLVKQKTDYGTASALLSVRDAIKDEEFLLIYGDILVDENAIEKVSSAAHNAILGVKVNNPRDFGVLNISEDGYLKNILEKPQNPPSNLINGGIYKLSSKIFEYIDKISKSVRGEYELTDAINLFSSENKIEIIKYDGLWMDIGKPWDIIDANKVLLDIEKPRILGEVEDGVKIKGKVIIEEGARVLHGTYIEGPVFIGKNSIIGPNSYLRPYTVLCGNNKIGASVEVKESVIMENTKVPHLSYVGDSVISEDVNFGAGTLVANLRFDEAEVKVFIKGERVSSGRKKLGTFIGAHVRTGINVSILPGVKIGAYAKIYPGAVVNRDVKKGEFYKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B1</accession>
    <name>F4B8B1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15e</name>
        <name evidence="6" type="ordered locus">Ahos_0824</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775626.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B1"/>
    <dbReference type="STRING" id="933801.Ahos_0824"/>
    <dbReference type="GeneID" id="10600291"/>
    <dbReference type="KEGG" id="aho:Ahos_0824"/>
    <dbReference type="eggNOG" id="arCOG04209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_1_0_2"/>
    <dbReference type="OrthoDB" id="8183at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000002">
        <property type="entry name" value="50S ribosomal protein L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_eL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_eL15_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="25835" checksum="B019E7818DCC4F77" modified="2011-06-28" version="1">MALSMYHYLAKVWQDEEWKKSVLRKRMIEWRNEENTVVRIEKPTRLDRARAIGYKAKQGFVVVRVRVRKGGLNKPRPNKGRRQKRMGVYGFSPSKGYRWIAEEKAARRYPNLEVLGSYFVGDDGLYKYYEVILVDPAHPVIKNDPNLKWLQDPANRRRVFRGLTAAGKKARGLLKSRGLKYTVRHKFVKKQKEREAKKRHEANKYYRLQNYDRIPGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G7</accession>
    <name>F4B8G7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">6-phospho 3-hexuloisomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G7"/>
    <dbReference type="STRING" id="933801.Ahos_0880"/>
    <dbReference type="KEGG" id="aho:Ahos_0880"/>
    <dbReference type="eggNOG" id="arCOG00068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094236_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03127">
        <property type="entry name" value="RuMP_HxlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443:SF1">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="5"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="173" mass="18829" checksum="DB38B4430F131DD6" modified="2011-06-28" version="1">MVDTLENFYKNNRNGKVLVMGAGRSGLVGRAFAMRLLHLGYNAYVLGETIVPAIGKNDLAIAISGSGRTRLILTAAEAAKEAKATLIAVTSYADSPIGKIADVVVEVPGRTKYSQFEDYFARQILGITEPLAPLGTLFEDTTQMFLDGVVAELMLRLKKTEEDLRQIHANIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J7</accession>
    <name>F4B8J7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="6">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha-IPM isomerase</fullName>
            <shortName evidence="6">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">leuD</name>
        <name evidence="8" type="ordered locus">Ahos_2078</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J7"/>
    <dbReference type="STRING" id="933801.Ahos_2078"/>
    <dbReference type="KEGG" id="aho:Ahos_2078"/>
    <dbReference type="eggNOG" id="arCOG02230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050075">
        <property type="entry name" value="LeuD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345:SF2">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="2 6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="7">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="154" mass="16790" checksum="EFBE1F9171FB2265" modified="2011-06-28" version="1">MKFGDKIDTDVIIPARYLKYTDPQYLAQHAMEPLDPEFYKKASSGVVIVAGKVFGMGSSREQAAIALKAAGVKAIIAESFARIFYRNAINNGLPVITLKDATKLIDEGEIVRINVETGEIEVNGRKYQGKGISGMALEILKSGGIMEYLKSGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J8</accession>
    <name>F4B8J8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cob(II)yrinic acid a,c-diamide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2079</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776865.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J8"/>
    <dbReference type="STRING" id="933801.Ahos_2079"/>
    <dbReference type="GeneID" id="10601579"/>
    <dbReference type="KEGG" id="aho:Ahos_2079"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_3_1_2"/>
    <dbReference type="OrthoDB" id="287850at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02145">
        <property type="entry name" value="BluB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012825">
        <property type="entry name" value="BluB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050627">
        <property type="entry name" value="Nitroreductase/BluB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02476">
        <property type="entry name" value="BluB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026:SF90">
        <property type="entry name" value="IODOTYROSINE DEIODINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026">
        <property type="entry name" value="NADPH NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="221" mass="25658" checksum="076C4EAB288E9D66" modified="2011-06-28" version="1">MDLYDAIKGRRDIRSYFKPDPIPDEVLAKILLAAHLAPSVGYSQPWNFIIIRDENIKRKVKEEVERQRLRYRELLDEGRRKLFDTIKIEGIMEAPINIAVTCDPSRFGPHVLGRITMPETCNYSTVLAIENLWLAARAENLGMGWVSFLDKNKIKEILGIPSNVEFIAYLTLGYVTKFPERPELEEKGWNYRLPLLEVVFEDRWGNKPNEKLLEKLNSAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L6</accession>
    <name>F4B8L6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="8">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">guaAA</name>
        <name evidence="10" type="ordered locus">Ahos_2097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776883.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L6"/>
    <dbReference type="STRING" id="933801.Ahos_2097"/>
    <dbReference type="MEROPS" id="C26.A31"/>
    <dbReference type="GeneID" id="10601597"/>
    <dbReference type="KEGG" id="aho:Ahos_2097"/>
    <dbReference type="eggNOG" id="arCOG00087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_4_2"/>
    <dbReference type="OrthoDB" id="33844at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000047">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001975">
        <property type="entry name" value="PRK00758.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="20990" checksum="98AF52ED769292EC" modified="2011-06-28" version="1">MKVGLVYYGGQYNHLILKNLKYLGVEVEVVNINADVEYPKKFDCMIFSGGPYSVDQEISKMGNSPLFVKELSVPKLGICLGHQLLAYVLGGKVGKANNPEFGLARITIDDHDTILQGLKKEFNAWESHNDEVKEVPPGFFAIAHSENTKVEAMVNKDSSIFGVQFHPEVKHTEDGIEIFKNFLTVCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S1</accession>
    <name>F4B8S1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD linked oxidase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775707.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S1"/>
    <dbReference type="STRING" id="933801.Ahos_0905"/>
    <dbReference type="GeneID" id="10600377"/>
    <dbReference type="KEGG" id="aho:Ahos_0905"/>
    <dbReference type="eggNOG" id="arCOG00345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_975268_0_0_2"/>
    <dbReference type="OrthoDB" id="34302at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD linked oxidase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="288" mass="33039" checksum="020D47D3A05F09C9" modified="2011-06-28" version="1">MEVYNEKELYNSIKDAYLSGKKVMIIGYGKHSNPRKSDIYLKIKMDYYKIEKEGYVEAYAGASVDKIREEASEHGLLLPCLYDGSIGGLLANNEFSPLSTRYGKPYDFTEKVFFITPFGKISWKIIIGSKGRLGAIYKARLKLFPKPTKVFTFEKSFNKKDETIAYVNKLMHLKPLAMLVEYDGKYTIHASYDFNADIHGFSKDEGVATIEETNRNGYYVNTPTIEDFMNAIEDTQPIYAYTVVGSGISKFYVADEDAIKKLNYFTHDDIPRVYWKLKAILDFKNIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T6</accession>
    <name>F4B8T6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme A transferase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775722.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8T6"/>
    <dbReference type="STRING" id="933801.Ahos_0920"/>
    <dbReference type="GeneID" id="10600392"/>
    <dbReference type="KEGG" id="aho:Ahos_0920"/>
    <dbReference type="eggNOG" id="arCOG05316">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069088_0_0_2"/>
    <dbReference type="OrthoDB" id="9252at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293">
        <property type="entry name" value="ACETATE COA-TRANSFERASE YDIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43293:SF3">
        <property type="entry name" value="CHOLESTEROL RING-CLEAVING HYDROLASE IPDB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="238" mass="26567" checksum="5D3E318DF17DC8E6" modified="2011-06-28" version="1">MIDHVIKAIALSLNDEEKVYVGLNSIPAILGAFMARDFYGKKIRILGVAEADNPSEIKITPSTGNPFYVNETPILPTVESFDLAQKGKLDVMFLGPAQIDEETNVNLSVIGGYSKPKVRLPGGAATAYILPLVKKAILWNLKHSKSTLVKRVDFVTGSAKFSMNKVIVVTNLGVLEYSREDKKWYVKYIYPWSNFAKIVENTAFQVYNAIQGVIDVNEEEKNFITKLDPDDLRSSLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U0</accession>
    <name>F4B8U0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FHA domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0924</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775726.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U0"/>
    <dbReference type="STRING" id="933801.Ahos_0924"/>
    <dbReference type="GeneID" id="10600396"/>
    <dbReference type="KEGG" id="aho:Ahos_0924"/>
    <dbReference type="eggNOG" id="arCOG05332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1088271_0_0_2"/>
    <dbReference type="OrthoDB" id="26617at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22680">
        <property type="entry name" value="FHA_ArnA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="155"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="233" mass="25336" checksum="992A807D98DAD3A0" modified="2011-06-28" version="1">MTWKCPICGYENSDDALFCMQCGAKKPENVKTEPPAVQQEANQQPPISAQPSAEPQAQQVQAQEQSSQPVVVTEQSNQQLTSEGQSSVQTSVKQPIEQSVQQTSQTPSQSVSVGSATQQTLQTPAGKYYLIFIATPVSALNKTKLPLEFDVFESISIGRSPENVIVIPDPEISRRHAIISLEGGKLYIEDLNSTNGTYLYDGKLFQPVKGKQELAPGSIIKLGSNTMLKIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V5</accession>
    <name>F4B8V5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mechanosensitive ion channel, MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0939</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775741.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V5"/>
    <dbReference type="STRING" id="933801.Ahos_0939"/>
    <dbReference type="GeneID" id="10600411"/>
    <dbReference type="KEGG" id="aho:Ahos_0939"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074283_0_0_2"/>
    <dbReference type="OrthoDB" id="31543at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049142">
        <property type="entry name" value="MS_channel_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21088">
        <property type="entry name" value="MS_channel_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel transmembrane helices 2/3" evidence="7">
        <location>
            <begin position="83"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="6">
        <location>
            <begin position="124"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="307" mass="33957" checksum="29345A158E7FFD79" modified="2011-06-28" version="1">MSRFNERQLIGLIVTVIISAIVIGLAIFTLANLKVIPANYTLYFYATTWFVGAVLVAYLVSEIVKSRLGNFIGASNATSVAFITRLLGYIIAFIGFFLLVHISIGAALAAGGFAGLVLGLASQTVLSNIFGGIMLLFSRPYKVGDRITISTWQYGLIAPTYPPKFFSNDFLIPGYTGVVTDISLLYTTIYTDDKVPVKIPNNIMVQAAIFIHNSEELRKIRSKYEVSKDLDPETVIKELKPRIEKIEGVLEVDIKVLETTFTTYILGIDTLAKSIFEEPIRDQVLRITMKTIKELQIGNGQKQLAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V7</accession>
    <name>F4B8V7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775743.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V7"/>
    <dbReference type="STRING" id="933801.Ahos_0941"/>
    <dbReference type="GeneID" id="10600413"/>
    <dbReference type="KEGG" id="aho:Ahos_0941"/>
    <dbReference type="eggNOG" id="arCOG07240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913989_0_0_2"/>
    <dbReference type="OrthoDB" id="34704at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="304" mass="34883" checksum="93BA148AA61DAF69" modified="2011-06-28" version="1">MVQIFIPTLGRETIKDTLNALKTQTFKDFEIILISKKQLNINGVNKIIIQKEGYFEEAINLALENLNPDDIALFTDDDAIPSSKWIEDHLEFHEKNPSIYIASGEVIGEKWKNYPNALFQRYKNTKYMMPYNSKFSEYTGFLTKVGLSVDRDDLGTYKVEKSLAIAGVNMSLKTNFFLEYKIPTFTLRGSYNESVLALHGIYLGGDSATFKGAKVYHKGNESLSRTKDPHIEKYLCLEKHVFPYAVNTIFKIDTNLLDEFISLLEEGIPKLGLSLALKGIVNNYTFLQFRKELLRLYELRETIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V9</accession>
    <name>F4B8V9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775745.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V9"/>
    <dbReference type="STRING" id="933801.Ahos_0943"/>
    <dbReference type="GeneID" id="10600415"/>
    <dbReference type="KEGG" id="aho:Ahos_0943"/>
    <dbReference type="eggNOG" id="arCOG08530">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177450_0_0_2"/>
    <dbReference type="OrthoDB" id="34636at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="12242" checksum="68F2753835DE0979" modified="2011-06-28" version="1">MDIGLDDIINVNLLKRKYEDYANSLTSGSNIKSVVKDFISFIKQIRLTTFSSKLLKILDEQERIANRILLVYNIRYLLLIFYKSIIQRMINKLINLIRSFLSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B903</accession>
    <name>F4B903_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B903"/>
    <dbReference type="STRING" id="933801.Ahos_2155"/>
    <dbReference type="KEGG" id="aho:Ahos_2155"/>
    <dbReference type="eggNOG" id="arCOG04351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511562_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019204">
        <property type="entry name" value="DUF2070_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09843">
        <property type="entry name" value="DUF2070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2070" evidence="2">
        <location>
            <begin position="10"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="511" mass="57740" checksum="4E1A479F686A5D2E" modified="2011-06-28" version="1">MPNARILLSIDVIEGAIIAFRGLEIGILFFYSFLIYSVSLLAILGKRIRTTLTLISIFSAVYLILSLFPIPYVFIFGTFIPLVNYSLLVDYNEKISVTLASIASLIPSIILADLTILGIVYVIAVGVSSYVYVALINRKGNKIVGISSLKIVRPFLRALNYNKSTELEEFLDKISIPNMLNILTVKINETYLILPQIHFGMYGEIGSSKFPYHVEEVIPNSFVFHGPGSHEIDLTSSKESKRIAQEILNTRFEKANFTGITQEMLGDFKITTLNFDKFTLSFVERPLKGIDDLPGALWRDMISTNNFLVDCHNETLIDEIGRKEYVTLKNFIYSKERYLGNRKLKIGYAEGEVNCEGLCKNKVRVLSFYSPEEDKKVSIIYLYANNACHGLREKIEQNLSDLTNAILVTPDDHSCTAMSFGNLYQPATLCEQLIISSRELVKESLEKMEDVKNIEYGILKVKTRVIGKVVSSMVDGLEKVGGFAMKTFWIPIILPYVILVVFLFIHEIFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B931</accession>
    <name>F4B931_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Archaeal flagella assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2183</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776969.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B931"/>
    <dbReference type="STRING" id="933801.Ahos_2183"/>
    <dbReference type="GeneID" id="10601686"/>
    <dbReference type="KEGG" id="aho:Ahos_2183"/>
    <dbReference type="eggNOG" id="arCOG01808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_443878_0_0_2"/>
    <dbReference type="OrthoDB" id="12374at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="entry name" value="Type II secretion system (T2SS), domain F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="500"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="138"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="393"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="612" mass="67410" checksum="50FB69042F70D09D" modified="2011-06-28" version="1">MAVKLKKKNKDAITLPKQYQLPIYASLVKLGIVKSMAKSYEQKLLMAGSSEDPQLFTAKIFFYLLLSSVLSVVLIGFGLFVIIKFYLVFRLAKYLALSFMMIIFGLIIPPVTYLANIATLSQKIESRRIGVDAELSAFTSVFAIFLRSGLTPRLMFDRLSSSSAFHYINSITLYVSKRIRYLGESVEDAMLHASQISPSKLLKDFFLAYVTAVRTGAPVINTIEAKAKDILDQLQLRASLAADRLSGIAESYVIWLSSGYIMFFLMMVLEAIFPVGGTSMIPLLGVLAVTVLPLVNIVFIYGVEQTQLRFPEKKLNYNLFFIFLAIGMIVMFVLLVLEKQIFYFLTLSGGTDNIAPTVIAMTIGLLIASVPPAIILTKELKEGTGYDPYVVGFMRAIAEGLRAGLPPETIIKNIKDSKEMGKFGKILNEIYAYITLGYPLKDAFRKGAERIMDFTSKISLVSLADMMEIGSMTPETVESIARQIESQIKIKRDYESKVKILLVTPYMGVILSLIASVLLGSAILSLLLGQKISYAVGPLIEASVLLPRAIYITAISSIFNSFMAGLLVGKLGSGKIATGLIHSAILVIITAVMLLVLLHVHFAFYKPSTPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B941</accession>
    <name>F4B941_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="5">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphopantothenate synthetase</fullName>
            <shortName evidence="5">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-4-phosphopantoate + beta-alanine + ATP = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B941"/>
    <dbReference type="STRING" id="933801.Ahos_2193"/>
    <dbReference type="KEGG" id="aho:Ahos_2193"/>
    <dbReference type="eggNOG" id="arCOG04262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078701_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="entry name" value="Phosphopantoate/pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010324">
        <property type="entry name" value="PRK13761.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695:SF1">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="167"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="236" mass="26179" checksum="C1A50E55C871A5CB" modified="2011-06-28" version="1">MVEALENNILVPQGLIAHGRGECFDYLIGEKTQDFAENAINAAAATLLLAKYPVISVNGNMAALVAKDIVELSNEVNCKIEINLFYRDEKRVKAIKKALENAGAKEVLGDDDLTTIPELFSERRKVSKNGIYIADVVLLGLEDGDRTEALVRLNKKVIAIDLNPLSRTSMTASITIVDNITRALPLLVKKVKELRSKDRIELVEIVKNFDNKRNLAEAIKFISRRLEALSEDLIGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B952</accession>
    <name>F4B952_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776990.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B952"/>
    <dbReference type="STRING" id="933801.Ahos_2204"/>
    <dbReference type="GeneID" id="10601709"/>
    <dbReference type="KEGG" id="aho:Ahos_2204"/>
    <dbReference type="eggNOG" id="arCOG05952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850668_0_0_2"/>
    <dbReference type="OrthoDB" id="36388at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15565" checksum="B0F926AB0AF483E8" modified="2011-06-28" version="1">MNSSALASEFLLRGTRTLKEATEAYNEGDLYYTLVRLDETLNNLASVILSLYGVYSVDGDSVNALSYLEETRDLDSSLKSLIKEIQDLDRQLSITNFIDESSLKSPSVVVRNAEVKTTLDRITKIFDKVQEVFDEFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B960</accession>
    <name>F4B960_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0967</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775769.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B960"/>
    <dbReference type="STRING" id="933801.Ahos_0967"/>
    <dbReference type="GeneID" id="10600439"/>
    <dbReference type="KEGG" id="aho:Ahos_0967"/>
    <dbReference type="eggNOG" id="arCOG00614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099718_0_0_2"/>
    <dbReference type="OrthoDB" id="86131at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052348">
        <property type="entry name" value="Metallopeptidase_M50B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864">
        <property type="entry name" value="ZINC METALLOPROTEASE MJ0611-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864:SF1">
        <property type="entry name" value="ZINC METALLOPROTEASE YWHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="6">
        <location>
            <begin position="55"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="209" mass="23374" checksum="BA0D516D699B07C1" modified="2011-06-28" version="1">MSKLDELEWRFRNINEIEAFLLAIFSISTFGIGKLIYSIIKGCPLYYSLLAVLFTLIAATIAVVPHEFAHRQVARKYGCFSRFTLSFSGFLATSIINLFGLAIVFFSGYTLISCNFFRTNKKLDGITATAGPATNLVISAIFYILASLFLPSLAGILFFYIAMFNSAVAFFNLLPFWVLDGMKIMRWNVKIWAAMIAASLVLMYLTGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A0</accession>
    <name>F4B9A0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A0"/>
    <dbReference type="STRING" id="933801.Ahos_1007"/>
    <dbReference type="KEGG" id="aho:Ahos_1007"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_659888_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="416" mass="48142" checksum="F451A7A2E66CA20F" modified="2011-06-28" version="1">MFNTLNMVNVIVNGKSKYNLDNNCQIYYGYFDLQDLVNSLSILDSSGYLRIRRMFAWNLYFHLSRNYSDQLRNLYYVIYEISAIIRELNFRNINPAYALSSILNNINTSMKLSWKNGEFSLKDPLEISCEIDLNAEINKLIKLEVEGKGKISVGENEIKSSNEMDLYKLSDVKGLSLESEFFYRDGKVNVKIDLKFRPENRGLNKEINAEDLNKVLRITAKEVNEKAINSHEFFEDFMVGIMETLIQPFLEKYKNELAKVLGFKEILYIPAGRHFILNAEESALNNEEIAQLFSDSCQLAINRIKEGKNSFFDNFSLPISVKGGLVFYEDSQITSLASRSIKSLATIILEVNSLKDQALVIIESPEEFLLNEDKSRIVNIVEKLMKKGNKIIIHTWDEKFLEYFKDCQVYKNEKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H5</accession>
    <name>F4B9H5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777036.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H5"/>
    <dbReference type="STRING" id="933801.Ahos_2250"/>
    <dbReference type="GeneID" id="10601756"/>
    <dbReference type="KEGG" id="aho:Ahos_2250"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811466_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="143" mass="16458" checksum="2E5B91075A1D4303" modified="2011-06-28" version="1">MNVKVVEAISEIGRNLFPSETVDALQGKVDKNELIKLRLDNAKFYLSQAKEINSPVIVSELLHKSLTEGFKALKDYFGIQKELKDSIPILSDILGNWIDEFWDLSLKLHYDGYIMEVIDIEDLKVYENKVSEFIQNCEIVVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M6</accession>
    <name>F4B9M6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M6"/>
    <dbReference type="STRING" id="933801.Ahos_1058"/>
    <dbReference type="KEGG" id="aho:Ahos_1058"/>
    <dbReference type="eggNOG" id="arCOG00500">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727135_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16871" checksum="4E8847397589C8E6" modified="2011-06-28" version="1">MKDVKIYSPPGFSKLLNTYKDIGNNISAEVYESNLPKAKIGDLEVYSVEACHAIYAVAYIITNGEKKIVYSGDTSEPCEGIIKESKDADLIIHEATCLEGCEIYGHTPLPKLKEIFDKKRVIITHIPSQIEDKYTNLGDFILAFDGLIWNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X1</accession>
    <name>F4B9X1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease P protein component 4</fullName>
            <shortName evidence="8">RNase P component 4</shortName>
            <ecNumber evidence="8">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnp4</name>
        <name evidence="9" type="ordered locus">Ahos_2316</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777102.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X1"/>
    <dbReference type="STRING" id="933801.Ahos_2316"/>
    <dbReference type="GeneID" id="10601824"/>
    <dbReference type="KEGG" id="aho:Ahos_2316"/>
    <dbReference type="eggNOG" id="arCOG04345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079140_3_1_2"/>
    <dbReference type="OrthoDB" id="10058at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE95187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="12559" checksum="2ADAF0347BB78BC5" modified="2011-06-28" version="1">MNKKTLKKRTLKLINDAIKLAEEGNLELARKYIMLAREYSNKGRFKIPLEYKRKFCRKCLTPLIPGKTERIRIKSKILVRSCLICGWIRRYDLRKNKENKSGTSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z4</accession>
    <name>F4B9Z4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777125.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z4"/>
    <dbReference type="STRING" id="933801.Ahos_2339"/>
    <dbReference type="GeneID" id="10601847"/>
    <dbReference type="KEGG" id="aho:Ahos_2339"/>
    <dbReference type="eggNOG" id="arCOG08320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663238_0_0_2"/>
    <dbReference type="OrthoDB" id="33779at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="414" mass="47439" checksum="80200A5BEE56FC82" modified="2011-06-28" version="1">MSILEVYSLQNKPIISCSLIDDNGNEKEILIISLEDNGIHVYKNIEEKDNHYILPPIPQIDLLIKEVIDEVAEELNVKSIVFKFGNNEEDEEQTDKLILSEEWYNAEKLALAASKHTALLSDIDSKIILGIVKFSSFLYAATILRKEDTFPLMQVVLKMDSEIPVLKIYNEMGQLVEERREKIDNFENYVRSLINSDEVAIVYKESLEEIPSPIEVTTNKGDKLYVGVIFKYFIGFLPSSTIKDREISIHNRKKLAKMLRALLYLDKMSKNSGTEIIIGRKGVPLTKLKEQINLIKNRVESMLHKLYNLNEINYYGINESVIDELIKYDEELSDGDLSLGIRVLPVAFIVTASNKQEFDNQMNRILNGPTSDGYDILDEYVRRNVSSYFIGYLMSLEEALIIYGDIINEMNNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA04</accession>
    <name>F4BA04_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777135.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA04"/>
    <dbReference type="STRING" id="933801.Ahos_2349"/>
    <dbReference type="GeneID" id="10601857"/>
    <dbReference type="KEGG" id="aho:Ahos_2349"/>
    <dbReference type="eggNOG" id="arCOG06040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1068006_0_0_2"/>
    <dbReference type="OrthoDB" id="35796at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="31270" checksum="B38FCE8115D44354" modified="2011-06-28" version="1">MDYIGLALKNGIDKEKAIYVYKILNGGYFMKLYYAKTPIINELKNWPTLYLKKKKYFPKIASPKYNEAMQLLITLDIYSILGTSFRLLKTTLEKKRLEDELKKVYDKISETCNNENIFPCPMRTFDVNTNQDFEPFIQDLFEKRLRDQKADIMSTIEEIAYNSEFFEELKKEVNWLKAIKVENTIRGIALAGKLEEFLDNIQDIVYLLSSERTLYFDALLLSNSIEDSIKKILEDGRKAIKNEINNEFSKDVNYIYALIRQQGSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA22</accession>
    <name>F4BA22_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775935.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA22"/>
    <dbReference type="STRING" id="933801.Ahos_1135"/>
    <dbReference type="GeneID" id="10600609"/>
    <dbReference type="KEGG" id="aho:Ahos_1135"/>
    <dbReference type="eggNOG" id="arCOG05994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243667_0_0_2"/>
    <dbReference type="OrthoDB" id="37140at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="12255" checksum="5F3AC55097AD1A6C" modified="2011-06-28" version="1">MKLRIDKLPKTDEDLEEIQQEIESEHHHHHHEEEGQSIEEVLGELYVRLQSLESKSKELEDSSEMCKEEISRIYKILGKMLIALTTKDENEKIKNLKDILSTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA56</accession>
    <name>F4BA56_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="7">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxS</name>
        <name evidence="10" type="ordered locus">Ahos_1170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775969.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA56"/>
    <dbReference type="STRING" id="933801.Ahos_1170"/>
    <dbReference type="GeneID" id="10600644"/>
    <dbReference type="KEGG" id="aho:Ahos_1170"/>
    <dbReference type="eggNOG" id="arCOG04075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_2"/>
    <dbReference type="OrthoDB" id="6840at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="9">
        <location>
            <begin position="47"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="7">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="275"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="336" mass="36922" checksum="ACF0D2ACC4BFF8F0" modified="2011-06-28" version="1">MRLYELSFNEIEEFFYKLAEVRDIIKDSGLMSFLPEKELPEIATGTARVKHAFPIFQKGGVIMDVTNVEQAGIAEDAGATSVMVLDKLPYDVRKSGGVARMADPKIIEEVMNSITIPVMAKVRIGHYYEAKLLEALGVDTIDESEVLTPADEEHHINKWEFKVPFVNGARNLGEALRRISEGASMIRTKGEAGTGNVSEAVKHMKIINAEIGALVGMSEEDRVKKAREYQVPYQLVELTAKIKRLPVVNFAAGGIATPADAALMMWLGGDGVFVGSGIFKSQDPAERAKAIVLAVAGWEYPEVVLEAQKMISEQKSMMGIDIKSLKPEELLQVRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA62</accession>
    <name>F4BA62_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">A-type ATP synthase subunit A</fullName>
            <ecNumber evidence="11">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpA</name>
        <name evidence="13" type="ordered locus">Ahos_1176</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The A chain is the catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775975.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA62"/>
    <dbReference type="STRING" id="933801.Ahos_1176"/>
    <dbReference type="GeneID" id="10600650"/>
    <dbReference type="KEGG" id="aho:Ahos_1176"/>
    <dbReference type="eggNOG" id="arCOG00868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_2"/>
    <dbReference type="OrthoDB" id="115235at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="224"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="232"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="590" mass="65665" checksum="AC488C138827C218" modified="2011-06-28" version="1">MVGKIVRVNGPLVVADNMRNSQMYEVVEVGELRLVGEITRIEGDRAYIQVYEDTSGLKPGEPVYNTGGPLSAELGPGLLGKIFDGLERPLSTIAEVTGSLFVNRGVKIPPLDRQKKWHFIPKVKKGDKVSPGDVLGVVQETSLIEHKILVPPDRHGEIKDIVPEGDYTVEDTIATLDMNGDQVSLKMMHKWPVRIPRPYKEKLEPSEPLLTGVRVLDTIFPLAKGGTAAIPGPFGSGKTVTLQSLAKWSSAKIVIYVGCGERGNELTDELRSFPTLKDPWTGKPLLERTILVANTSNMPVAARETSIYLGVTLGEYFRDQGYDTLVVADSTTRWAEALRDLGGRMEEMPAEEGFPSYLPSRLAEYYERAGRVITLGNPERLGSVTLASAVSPPGGDFTEPVTSNTLRFVRVFWPLDVSLAHARHFPAINWLQGFSAYVDLVGKWWNTNVDPQWKEMREFMVKTLIRENELQQIVKLVGPESLAEKDKLVLEVARLIKEAFLKQNAFDDIDAFSSPQKQVRIMKLIYLYNSLASPLVEKGVPVKKIIDNIKVVSDIIRSKATIPNNALEKYDELEKTLRAQFEQLSKEVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA82</accession>
    <name>F4BA82_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH (Or F420H2) dehydrogenase, subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1196</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775995.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA82"/>
    <dbReference type="STRING" id="933801.Ahos_1196"/>
    <dbReference type="GeneID" id="10600672"/>
    <dbReference type="KEGG" id="aho:Ahos_1196"/>
    <dbReference type="eggNOG" id="arCOG01551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_6_1_2"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NADH:ubiquinone oxidoreductase, 30kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004734">
        <property type="entry name" value="PRK06074.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10884">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="Nqo5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase 30kDa subunit" evidence="3">
        <location>
            <begin position="35"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="165" mass="18721" checksum="B7F893CD8FB31C20" modified="2011-06-28" version="1">MSQTTVQKPIDKVLAELKAKGIIAKAESESRGSLEVTKNKIIEIAKIMKELGFDHVIAVTGIDFPEQQKIQVIYHVSSYSVEDLQKIIFAIKTYVDYKDPSLPSLTQIWGSAWTGEKETYEMLGVRFEGHPELSRLFLPEDFEGVYPLRKDFKIKLEGLFVDKPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L2</accession>
    <name>F4B3L2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NfeD-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774818.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L2"/>
    <dbReference type="STRING" id="933801.Ahos_0006"/>
    <dbReference type="GeneID" id="10599446"/>
    <dbReference type="KEGG" id="aho:Ahos_0006"/>
    <dbReference type="eggNOG" id="arCOG01912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044513_0_0_2"/>
    <dbReference type="OrthoDB" id="28112at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052165">
        <property type="entry name" value="Membrane_assoc_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507:SF4">
        <property type="entry name" value="NODULATION COMPETITIVENESS PROTEIN NFED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141322">
        <property type="entry name" value="NfeD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD-like C-terminal" evidence="6">
        <location>
            <begin position="60"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE92901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="13768" checksum="8688B431B62A1705" modified="2011-06-28" version="1">MAHHIVIPIIIILVLVIVLIATGYISDPIIDIPSFAIVGFLTYRIFYVIIKTRKRNLYSYTGKIGKAVEDIPKGKEGYILIEGEYWRAIAEEPITSGDEVIVVGMQDLKLIVRKNKNNEVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q4</accession>
    <name>F4B3Q4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="9">4.1.99.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1208</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776007.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q4"/>
    <dbReference type="STRING" id="933801.Ahos_1208"/>
    <dbReference type="GeneID" id="10600684"/>
    <dbReference type="KEGG" id="aho:Ahos_1208"/>
    <dbReference type="eggNOG" id="arCOG02741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="OrthoDB" id="335406at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070284">
        <property type="term" value="F:phosphomethylpyrimidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="442" mass="49119" checksum="27E9854A38A59A2B" modified="2011-06-28" version="1">MATQITEAKSGNVTEEMKIIAKLEGESPEKIRDRVAKGKVVIFKNIVRQNLERYTAIGEGLFTKVNVNLGASTDHYDENEELEKVEIANKFGADTIMDLTDGGDIDGMRRKVLAKAKMPVGTVPIYQLYYEMVTKRKYVIDFNEDDLFNVIEKHFKDGVDFVTVHTGVTLDLSKKAAEQKRTAGIVSRGGTILAAWEIYNEKENPLYANFDYLLELAKEYDVVLSLGDALRPGGIDDAHDELHVGELLVNARLAKKAIEKGVQVMIEGPGHMPLDQIEMDIKLEKQLTGGVPYYVLGILPTDIAAGYDHIAGAIGGALAAAYGADMLCYLTPAEHLSLPTPEQVKDGLIAFKIAAHTGDIIKLGEKARKLDTEMSKARASLNWQKMFSLTFDEERARRIYRQYKKFEAGSCTMCGDLCVYLVLPRALNKKKYANQKASTENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q5</accession>
    <name>F4B3Q5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rieske (2Fe-2S) domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776008.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q5"/>
    <dbReference type="STRING" id="933801.Ahos_1209"/>
    <dbReference type="GeneID" id="10600685"/>
    <dbReference type="KEGG" id="aho:Ahos_1209"/>
    <dbReference type="eggNOG" id="arCOG02853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_3_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496">
        <property type="entry name" value="FERREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21496:SF0">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="103" mass="11622" checksum="D79CAA27C9FDE69C" modified="2011-06-28" version="1">MQIKKPQLKIGEKTKIKANINGEEKEIVLLYLGGDKYIAFDAYCPHLGCDLEKYGVLIREEIVCQCHFSHFSIKDGKPTKGASKKPLKIYQVKVGKNELIIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q9</accession>
    <name>F4B3Q9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11 12">Chorismate synthase</fullName>
            <shortName evidence="11">CS</shortName>
            <ecNumber evidence="3 11 12">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aroC</name>
        <name evidence="14" type="ordered locus">Ahos_1213</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="11 13">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11 13">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 13">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="3 11 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776012.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q9"/>
    <dbReference type="STRING" id="933801.Ahos_1213"/>
    <dbReference type="GeneID" id="10600689"/>
    <dbReference type="KEGG" id="aho:Ahos_1213"/>
    <dbReference type="eggNOG" id="arCOG04133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_0_0_2"/>
    <dbReference type="OrthoDB" id="33049at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.150.10:FF:000002">
        <property type="entry name" value="Chorismate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11 13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11" id="KW-0288">FMN</keyword>
    <keyword evidence="10 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="301"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="390" mass="42313" checksum="35B077FE599CD6D0" modified="2011-06-28" version="1">MPGNSLGKALTITTFGESHGPAIGVVIDGIPAGLPLSKEDIEFELSFRRPGKLFVSGRREKDEPEILSGIYNGRTTGAPVAIIIRNTDVISSLYEEVHYKPRPGHADLPYIMKYGFENWDYRGGGRASARETATRVAAGAIAKKLLMLTNTVIAGHLISLGNVELEEKVSFEDILCSKYSPVRASKKSLEEKYEKLLMEATKEGDSYGGVAEIVVNNPPIGLGEPVFDKIKADLAKAVMSIPAVVGFEYGLGFKAAHMRGSEANDEIVLKDGKLGWKYNNAGGILGGLTNGEQIILRCAFKPTSSIRKPQKTIDLRTMKETEISVIGRHDPAVAIRGVAVVEAMVALVLADHAIRAGIIPQVRLDDKQASLIEENWKRYVNACRPTEGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U1</accession>
    <name>F4B3U1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="11">RPPK</shortName>
            <ecNumber evidence="11">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5-phospho-D-ribosyl alpha-1-diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="11">P-Rib-PP synthase</shortName>
            <shortName evidence="11">PRPP synthase</shortName>
            <shortName evidence="11">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">prs</name>
        <name evidence="15" type="ordered locus">Ahos_1245</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776044.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U1"/>
    <dbReference type="STRING" id="933801.Ahos_1245"/>
    <dbReference type="GeneID" id="10600724"/>
    <dbReference type="KEGG" id="aho:Ahos_1245"/>
    <dbReference type="eggNOG" id="arCOG00067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="OrthoDB" id="371997at2157"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000074">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002095">
        <property type="entry name" value="PRK00934.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="13">
        <location>
            <begin position="150"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="221"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE94128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="292" mass="32265" checksum="0BDCCEBDA76BB891" modified="2011-06-28" version="1">MIIVGGPASNGIDEKLSEMLNVKLVKAEHKIFPDGESYIRIPESVRGEEVVIVQSTYYPQDKHLMELLLMIEAVKNLGASKIISIIPYLAYARQDRRFKDGEALSLKIVLNLIYESGSDELITVEPHKPDAVISYFKGNVKIVDPTPALAREIKKEVENPFVLAPDRGALERAERLAKELNSPYSHIEKERDRTTGEVRIKEAPHLDLHGKDVVLIDDIISTGGTLAQASQLSYNLGARKVIAAATHVLLVNDAYDKLTRAGIKSIIGTNTIKVENKNVKIADISDLIAVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V5</accession>
    <name>F4B3V5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776058.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V5"/>
    <dbReference type="STRING" id="933801.Ahos_1259"/>
    <dbReference type="GeneID" id="10600740"/>
    <dbReference type="KEGG" id="aho:Ahos_1259"/>
    <dbReference type="eggNOG" id="arCOG00891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_5_2_2"/>
    <dbReference type="OrthoDB" id="26412at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46124">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46124:SF2">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="225" mass="26142" checksum="1A40AB84379ADAD8" modified="2011-06-28" version="1">MLYDAHCHYSLLKKKYEGYFIAAVSMDYNSSVETLSLNDKNILKGVAIHPWNAHKEKIESVLPLIDKADFVGEIGLDYKYSEAPKELQIRYFEEFLKKSQDKTVNIHAVNSWNDVLNLLIKHDIRRAIIHWYSGPKELLRDIEGAGYFITINPSVTFQEKHRIIAENASPNIVLTESDGGYMYKGKLLEPTDIPSIIKFLSKIWNEDERYVEKKIEINFRKAFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F4B410</accession>
    <name>F4B410_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA sulfurtransferase</fullName>
            <ecNumber evidence="11">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thiI</name>
        <name evidence="16" type="ordered locus">Ahos_0081</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H(+) = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[ThiS sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [ThiS sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="Rhea" id="RHEA-COMP:19908"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774891.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B410"/>
    <dbReference type="STRING" id="933801.Ahos_0081"/>
    <dbReference type="GeneID" id="10599521"/>
    <dbReference type="KEGG" id="aho:Ahos_0081"/>
    <dbReference type="eggNOG" id="arCOG00038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037952_4_1_2"/>
    <dbReference type="OrthoDB" id="372227at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA-uracil-4 sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11716">
        <property type="entry name" value="THUMP_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054173">
        <property type="entry name" value="ThiI_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049961">
        <property type="entry name" value="ThiI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049962">
        <property type="entry name" value="THUMP_ThiI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00342">
        <property type="entry name" value="tRNA uracil 4-sulfurtransferase ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22025">
        <property type="entry name" value="ThiI_fer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="15">
        <location>
            <begin position="58"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="389"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="11">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE92974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="467" mass="52721" checksum="B2A6D62396B78D3E" modified="2011-06-28" version="1">MKLLIVRYDEIGVKSDIVRKKLEKLLIDNLKASASYFKCGEVKAEKGQGRIYLYGNIDCLKISSSKVFGVKSVSPAEKITFQNLNEIIDFAEKLWKNKIEGKRFAIRVRRVGKHDFTSIEAAARIADRFNGKVDLENPEVKVFVEIRQNEAYFYDEIIKGPGGLPLGSEGKALALVSGGIDSPVASWMIMKRGVTLDILHCNISGPNNLSSIMKVIDKIKEWSQGYTPRVFIVDCGKIMEAIMKKVDIRLWSVAFKRALYLIAINYADKIGAKAIVTGESLGQVSSQTLSVLSGLQHKIDLLFLRPLIGFDKDEIVKLAREIGTFELSTKVPEYCALFSHKPRTKVTIEEIEKIDEALKEAVEETLKESEQMQTENYNVQIYLDKLPTSLQNTVIIDLRKEEEYDKSHLPNAIRLDPWEVMDFVLKTGKDKTYILYCEKGIVSGDLAYRLKKMGYNAFALKNPTLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B475</accession>
    <name>F4B475_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF434 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="205" mass="23389" checksum="04165B30DE2B5C7D" modified="2011-06-28" version="1">MILRKELKEAYIDYKYLLNRGYNRKPALDLVTGRYSLSFKERLLLYRCTHSDEEIKEIKEKTILEKNVVIDGYNVAITILNALDNDEAFICDDGFVRDLGLGSKKGDDRIEDILILFAEFCYSLGLSFTIILDSQISHSGELASNLRKRGINAKTVNKADKTVMSSELTIISNDFVIMKNSVKLFDLVGLFLKGYIYLPKIPEDI</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="379310at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="165" mass="17883" checksum="1D7EFEC1A1412A68" modified="2011-06-28" version="1">MHILTKIGLIALVIGIILAGIGFYEFSSLVNEMEPVIELHCAQRITLGPYQNYTISVSPSPGRIIAFAYNSTSPIDVTLPSSFTNSTSPSTAERVYLSPTGDISGIIEFHNPSNSSIVVYYILKYIEVSTIDVYSLAFVLLGGLLFIVGLIIAIIGLVIGRKKRT</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="STRING" id="933801.Ahos_0134"/>
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    <dbReference type="eggNOG" id="arCOG03117">
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    </dbReference>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
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    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF6">
        <property type="entry name" value="UNDECAPRENYL PHOSPHATE TRANSPORTER A"/>
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    </dbReference>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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    </evidence>
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            <dbReference type="Pfam" id="PF09335"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="22231" checksum="F11C136EDC00BBE2" modified="2011-06-28" version="1">MYEFTSQLSYIVIFILMILEGMSLPIPSEIIMPLVGYYSFKGYINIEIGIISGTIGSLVGSLIDYFIAEKLGIPFLSKYGKIFGIDDNKLNVLGTWFDKYGIFAVFGFRFIPLFRALISFPAGLARMKIVKFILATFSGHIIWNTALALIGYEFATQWKMIINQIEKLGYIIAGIIILVIVAYIIAIFIKRRRNLR</sequence>
</entry>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001667">
        <property type="entry name" value="PRK00431.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
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    <dbReference type="PANTHER" id="PTHR11106:SF111">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="A1pp"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="172" mass="19201" checksum="C2DC5B1CB26AB529" modified="2011-06-28" version="1">MIVYLTKGDITEIEADVIVNAANSFLEHGGGVAYAIVRKGGKEIQEESREYVRKHGPLKTGEVAVTSAGKLKAKYIIHAVGPRYGIEGEEKLEEAIRNSLRKAEELKVKSIALPAISTGIYGYPYEVCARKMAKVLKENWNLDKIIVCLYANYAYDVFKRIFKEEGINFIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F7</accession>
    <name>F4B4F7_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">HEPN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774963.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4F7"/>
    <dbReference type="STRING" id="933801.Ahos_0153"/>
    <dbReference type="GeneID" id="10599593"/>
    <dbReference type="KEGG" id="aho:Ahos_0153"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009977_0_0_2"/>
    <dbReference type="OrthoDB" id="39912at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="14537" checksum="CB499BA2ABDB56BF" modified="2011-06-28" version="1">MDKFLEFLEASIEEFNKGRYRVSCLLCQVSAELLIRSIFDERGLKQPIVPSHDIRTLLGRLNDESLYEFIKENRRELDVISNCRKNSQYGEVKKEEAEECIKMVKFLFEKLKGVIDADRINFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H7</accession>
    <name>F4B4H7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide/dipeptide ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0174</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774983.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H7"/>
    <dbReference type="STRING" id="933801.Ahos_0174"/>
    <dbReference type="GeneID" id="10599614"/>
    <dbReference type="KEGG" id="aho:Ahos_0174"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
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    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="8"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="326" mass="36527" checksum="EA214F198E554B57" modified="2011-06-28" version="1">MQADNALLNINNLTLHYYTKNLEISAVDDVSIYVEKGKVLGIAGESGSGKSTLAKAIFKILPSSAKILKGEIIFDGKNILDMDEKTFSKEIRWKRISYIPQVSMNSLDPVYKIKYQLLETIFVHEDISRAEALERIEKLITSVGLPLEILNKYPHELSGGQKQRVMIAMALLLNPDLVIADEPTTALDVIIQAQIISLLKNIQQEKKLSMVFITHDLSLLAQVSDMIAIMYAGRIVEFGSVEDIYYNPKHPYTKLLLKSVPNIKDRKRKLESIPGEVPDLANPPKGCRFHPRCPLAQDICRKENPSPIRVGPNHYVSCFMVGEGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H8</accession>
    <name>F4B4H8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide/dipeptide ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774984.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H8"/>
    <dbReference type="STRING" id="933801.Ahos_0175"/>
    <dbReference type="GeneID" id="10599615"/>
    <dbReference type="KEGG" id="aho:Ahos_0175"/>
    <dbReference type="eggNOG" id="arCOG00184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE/OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43230:SF3">
        <property type="entry name" value="ABC-TYPE DIPEPTIDE_OLIGOPEPTIDE TRANSPORT SYSTEM, ATPASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="314" mass="35319" checksum="0A68D248577944EC" modified="2011-06-28" version="1">MLEAKEVTVLFSARGIVIPAVDEASITAEKGKIIGLVGESGSGKTTLLRVLLGLQKVDKGEVLFNGENIFKLKSRKLKEFRRLNQIIFQDPFDSMDPRFRVYDIIAEGLRYHKIAKDKNEEKEIIYNTLEEVGLHPADKFALKYPSQLSGGQLQRVAIARALALNPEIIAADEPVSMLDVSIRASILNIFINLKQEKNVGFVIVSHDISTLSYISDEIYVMYMGRIVEKGNVDEIIEKPMHPYTQALIASVPVADPKYKIEKIPIKGEISYDHFKYGCRLYPRCPFAMDICAKETPPLIEVSPGHYVACHLIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K8</accession>
    <name>F4B4K8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6 7">Acylphosphatase</fullName>
            <ecNumber evidence="2 6 7">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1355</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the acylphosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="2 6 7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K8"/>
    <dbReference type="STRING" id="933801.Ahos_1355"/>
    <dbReference type="KEGG" id="aho:Ahos_1355"/>
    <dbReference type="eggNOG" id="arCOG01674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141932_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000012">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011000">
        <property type="entry name" value="PRK14426.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011007">
        <property type="entry name" value="PRK14433.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011012">
        <property type="entry name" value="PRK14440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268:SF4">
        <property type="entry name" value="ACYLPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00112">
        <property type="entry name" value="ACYLPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00151">
        <property type="entry name" value="ACYLPHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="9">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="91" mass="10529" checksum="3ED41566E73C1DFC" modified="2011-06-28" version="1">MLLRRMKAKVYGRVQGVGFRRFVQINAIRLGIKGYAKNLDDGTVEVVAEGYEEALEKLLDYLRQGPPLAKVEKVDYEMEDYKGEFDSFETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L3</accession>
    <name>F4B4L3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776158.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L3"/>
    <dbReference type="GeneID" id="10600847"/>
    <dbReference type="KEGG" id="aho:Ahos_1360"/>
    <dbReference type="eggNOG" id="arCOG07220">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433936_0_0_2"/>
    <dbReference type="OrthoDB" id="41237at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="91" mass="10411" checksum="41ECB75D1C93DB0A" modified="2011-06-28" version="1">MRAFEAKICGEKLILQNKSGKTLFIREILIKYKVTVTTVDEKTALKTITDSIKINRDVQDKIEIPLVSTDVAEISVIYRQDEITLREDISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q1</accession>
    <name>F4B4Q1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Box A-binding protein</fullName>
            <shortName evidence="7">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA sequence-binding protein</fullName>
            <shortName evidence="7">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tbp</name>
        <name evidence="9" type="ordered locus">Ahos_1398</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q1"/>
    <dbReference type="STRING" id="933801.Ahos_1398"/>
    <dbReference type="KEGG" id="aho:Ahos_1398"/>
    <dbReference type="eggNOG" id="arCOG01764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001592">
        <property type="entry name" value="PRK00394.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="74"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="167" mass="19052" checksum="B74E3A6C48CD1F45" modified="2011-06-28" version="1">MERSVPNVEYDPDQFPGLIFRLESPKVTSLIFKSGKMVVTGAKSTEELIKAVKRIIKTLKKYGIKITGKPKIQIQNIVASANMHVHVNLDKAAFLLENNMYEPEQFPGLIFRMDDPRVVLLIFSSGKMVITGAKREEEVYKAVKKIFDKLNELDCIKPIEEEEELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q2</accession>
    <name>F4B4Q2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776197.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q2"/>
    <dbReference type="STRING" id="933801.Ahos_1399"/>
    <dbReference type="GeneID" id="10600888"/>
    <dbReference type="KEGG" id="aho:Ahos_1399"/>
    <dbReference type="eggNOG" id="arCOG01150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075478_0_1_2"/>
    <dbReference type="OrthoDB" id="18264at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07391">
        <property type="entry name" value="MPP_PF1019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="248" mass="28211" checksum="1F8B7C57314B3686" modified="2011-06-28" version="1">MIEIEKGIYIEGDIPGIYIKSINTAVISDVHIGYEEEMARKGIFLPRVQKKKFLDVVNKIIREFNTYNIIVDGDFKHIFNGLGKQEKEDLTSALSTIKDLGVKLTLIRGNHDNYISLVTDKFDIEIRDEIETDNMIILHGHKDIEPKDNVIYIIGHEHPRISLRDKLGFSRKLQAFLLVPLKNGSKALILPAIGSYQAGNDISLIHNNYMSPLIRNYGILEEAKPYVIIEGEGIMEFPKLELLKNIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q9</accession>
    <name>F4B4Q9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic divalent cation tolerance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776204.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q9"/>
    <dbReference type="STRING" id="933801.Ahos_1406"/>
    <dbReference type="GeneID" id="10600895"/>
    <dbReference type="KEGG" id="aho:Ahos_1406"/>
    <dbReference type="eggNOG" id="arCOG04231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098807_3_1_2"/>
    <dbReference type="OrthoDB" id="8015at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF8">
        <property type="entry name" value="FI09726P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="105" mass="11801" checksum="5AAD3C977DEB592D" modified="2011-06-28" version="1">MTAIVVLTTISGLESGKKLARSLVEEKLAACVNIIPFVKSTYRWEGKVVEDDESLLIIKTDSSVKEKIIKRIKELHPYELPEIITLDVTGGLENYLNWIAESVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S0</accession>
    <name>F4B4S0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="15">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rtcB</name>
        <name evidence="16" type="ordered locus">Ahos_1417</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14 15">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="15"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776215.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4S0"/>
    <dbReference type="STRING" id="933801.Ahos_1417"/>
    <dbReference type="GeneID" id="10600908"/>
    <dbReference type="KEGG" id="aho:Ahos_1417"/>
    <dbReference type="eggNOG" id="arCOG04246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 15 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="12">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </evidence>
    <sequence length="372" mass="41773" checksum="2DB1217B975E2D14" modified="2011-06-28" version="1">MLESIKPMSKGQEELLNALTNSNYNIIGIFGPTGTGKSLFSLAYSIDSVSTGKFRKLIVAKPIVDVVTQEELTRKEYDKYEDMVKDYIKDVLGGFAEEKTIDDLFSSGKIEVLDSRYLRGRSFNDSIIFLDDVQLMKPESVLELFIRAGKNSRLIIAGDPVFQTLSNEADSSEIIREVLLNEKDVKVVDLGIKDIVRAGTKRGIRLLLEYKLRSRKLSEAEKKVMDSAKIRAPDADIITVVEFSEEKKKLNITSEHVPDALIVVKEGNAGRLIGKSGERINGIESDTKMKVRVVELKLDFKDIIRAVHPLPWVVKHVDDVDFQGNELVVRLKKESGGFIGQKGVNIRLVEYVIKQMFNVGVRVIQPSEENQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X7</accession>
    <name>F4B4X7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775059.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4X7"/>
    <dbReference type="STRING" id="933801.Ahos_0250"/>
    <dbReference type="GeneID" id="10599690"/>
    <dbReference type="KEGG" id="aho:Ahos_0250"/>
    <dbReference type="eggNOG" id="arCOG04138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025558_0_0_2"/>
    <dbReference type="OrthoDB" id="85892at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="559"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="105"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="614" mass="71371" checksum="6C8721B54B34CC2E" modified="2011-06-28" version="1">MIIPEPMLKIYILFPSDLSEQISACIVKFGDFQPEEGKYFERASYMEQVSFVNTMLDEVSKIIDFFNIGYKKEGIVEFNVIEKNLEEFRIESKFIDSIYQKIFSYNKYLDERARLYDEIEETRSEEKLIELDKVIHEITELHKELNTERKAIEKRIMEDYNKLLTLKEILKILTSVRFSKYYAYLEGYIREKRKDEFLRVMSKYALIIFERPKRFGSKEIPPTYSSVPHFMRPFESIVGIYGTPSYWEVDPTFLFAITFPLFFALMFPDAGDGLVLLLFSILFYKYAINKNNQQLKDISIVLAYSSVLSMIIGMLLREFFGPLFVEGTKELVPQSNESTIGPLYYYWPVPPDVSKIFSSIIPFGQYSQPLYGIQNAIIIAILIGIILTLIANSLGIYNSHLKSTKEDILYNKIPNFIVYLSPTIIFIYGFYNPYKYFINTYNLLGALLYTLMHLYSPIFYGYYLVSFISIIIFFIGEIYGWISHIYINIREGLKNALLKGFIEGFFSPLLLVISNVISFIRLLVLGLSHYYILYAFSFIAVMLAGTLDTWRILSNPMSIIILILGNILVIGLEGFVAVIQAIRLNLYELGGKFCECEGRPFENVKEYAIIKNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B548</accession>
    <name>F4B548_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1467</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776265.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B548"/>
    <dbReference type="STRING" id="933801.Ahos_1467"/>
    <dbReference type="GeneID" id="10600965"/>
    <dbReference type="KEGG" id="aho:Ahos_1467"/>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_4_2"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="67"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="488"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="505" mass="53675" checksum="09FC3846F2B9A85E" modified="2011-06-28" version="1">MDTKYLKIISLGIFVIPFLLMIITSASTTNVTQEIQSLNQSIAALANRTSDYPPASVPSWLSLGSNAWMLTAATFVGLQSVPGVALYYAGLSKKKYAVNSALMVFYAFAIVLVVWMIAGYNFGFGKPTLVINGYGILGTPLPAWPGTYEASQTIYGPSNSMLNIPTSTYIFFQFVFAAITPVLLAGGVLERMNFKAWMVFVPFWSLLVYSPVAYWLFAGGWLNQLGAVDFSGGYVIHVDAGVGALAAALAVGPRLASERKLEAHSLPLILAGAGLIWLGWDGFNGGDPGGATIDAAIAVLNTNIATAVSAVTWMLMDMAFFKKPTLVGATSGAITGLVAITPAAGYVNGLYSIIIGIASGSIPWLALYKLEPKLKVDDTLGVFSTHGIAGIVGGLLTGIFADPNVTVYVDPTLRGALYGNWYQLGIQAFAAAVVFVYDFAITFGLLKLIGLFIPLRAPPETLQIGDYAMHGEVAYSELLATLPAEQKPAAEKVEEEPSKKKEEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B550</accession>
    <name>F4B550_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidinotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776267.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B550"/>
    <dbReference type="STRING" id="933801.Ahos_1469"/>
    <dbReference type="GeneID" id="10600968"/>
    <dbReference type="KEGG" id="aho:Ahos_1469"/>
    <dbReference type="eggNOG" id="arCOG03108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049514_0_0_2"/>
    <dbReference type="OrthoDB" id="371705at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016990">
        <property type="term" value="F:arginine deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019546">
        <property type="term" value="P:L-arginine deiminase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003876">
        <property type="entry name" value="Arg_deiminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47271:SF2">
        <property type="entry name" value="ARGININE DEIMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02274">
        <property type="entry name" value="ADI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01466">
        <property type="entry name" value="ARGDEIMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="417" mass="48389" checksum="4EDA0E9EDD882E53" modified="2011-06-28" version="1">MLRITSEWSKLRTVLVHEPGIEMFYGILDPDAFLYMRRFNVERAINQHKQMVNKLKEMGIEVLKIKELINEKAKSDKEFRKLLEKIALNYIKYEGDGKAEENLSRVKKDLEKLDSDTLFNIILLNPTVLSHEALGTNEAVTRILNEEPLANLYFTRDQAIITDKGVVIGRMSKRIRMRETEIIKLIFQALYEKPLKEISEPAFLEGGDYMPFKDFAIFGTGDRTSISGIMQAIDFTNFNEIVIAYNPEIEETEDYMLTMHLDMYLNSPKEGVVISNSTILSKTLAHIYERKENGISLKERTNLLEFFKKKGYRILEISLAEQLSYATNFLTIENGRILSPKVVNNMINIMEYLERKGYNNLLNEVKEDYNRHITNKDFFPNRKDIKDEGIDYDEIDVSELTGGFGGIHCMTMPIKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B564</accession>
    <name>F4B564_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">HhH-GPD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1483</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B564"/>
    <dbReference type="STRING" id="933801.Ahos_1483"/>
    <dbReference type="KEGG" id="aho:Ahos_1483"/>
    <dbReference type="eggNOG" id="arCOG00461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF19">
        <property type="entry name" value="DNA REPAIR GLYCOSYLASE MJ1434-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="11">
        <location>
            <begin position="29"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="23992" checksum="E616C6C9890DC32F" modified="2011-06-28" version="1">MGWIVSDPYSFEWWGGLKSADEIAISAFLVQMTKWETVAKVIENLRRKGLNYIDKIAELPLPVLEDQIRSVNFYKTKARRLKNFSEIVSKNGGLKKFLTVENRDKILEIEGIGEETADSLLLFANNQLVFPQSEYLRRVLSRVLNKKMSKKEAKDYVEDNLKKDLFLYKLFHAGIVSIGKAFCYLNKPKCDKCILKPLCRNKYNETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B585</accession>
    <name>F4B585_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sugar transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0288</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775095.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B585"/>
    <dbReference type="STRING" id="933801.Ahos_0288"/>
    <dbReference type="GeneID" id="10599728"/>
    <dbReference type="KEGG" id="aho:Ahos_0288"/>
    <dbReference type="eggNOG" id="arCOG00157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_3_2"/>
    <dbReference type="OrthoDB" id="45815at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050809">
        <property type="entry name" value="UgpAE/MalFG_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43227:SF7">
        <property type="entry name" value="ARABINOOLIGOSACCHARIDES TRANSPORT SYSTEM PERMEASE PROTEIN ARAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43227">
        <property type="entry name" value="BLL4140 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="72"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="297" mass="34112" checksum="953ABB3CBDCAF2B7" modified="2011-06-28" version="1">MTSSKNGKNEDNGFLYILPILILTIFVFILPLVYALYISFTNMNLFHLFNYNIVGLKNYAIIFECGYFWELIKNTLIWTLGSLVTMMSLGFLLALILNQRDLKGKSIFYAFLILPWAFPGFISLLIWQGLWVDPYGMMNKLILPMLHLPRINSLISTTDAWIELILTNDWLSFPYFMTVFYSSLQSIPHELYDIAELDGAGTLTKFFRITLPSLKRTIAFVFITSFVFTWNNFYPIFILTGGGPGISTETFIVYAYQEAFDYSNFALASAWSVISTIFVIILGLIVIKYTHILDSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C6</accession>
    <name>F4B5C6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6-phospho-N-acetylmuramidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C6"/>
    <dbReference type="STRING" id="933801.Ahos_0329"/>
    <dbReference type="KEGG" id="aho:Ahos_0329"/>
    <dbReference type="eggNOG" id="arCOG06045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302563_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008589">
        <property type="entry name" value="MupG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043797">
        <property type="entry name" value="MupG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38435:SF2">
        <property type="entry name" value="DUF871 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19200">
        <property type="entry name" value="MupG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phospho-N-acetylmuramidase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="211" mass="23306" checksum="C99980622DBECF42" modified="2011-06-28" version="1">MILKGEELGFSEIFFGIGRGVHTRSSIEALANAKELLKIANKLGYYSFVDINPSILEELGASPRNLSIFKEAGFSAVRVDCGFSVDDILKIRDIGIELNAYTFPEDKIEYLLRNIDPEKVKATHNYYPVIGSGISIKELIEKSKPFVKSGIPVGAFVSVPSVRSDTTVEELRDKNPRESARILFSTGVISRVLIGDAHPTDEEMIDLSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E4</accession>
    <name>F4B5E4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-galactosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5E4"/>
    <dbReference type="STRING" id="933801.Ahos_0347"/>
    <dbReference type="KEGG" id="aho:Ahos_0347"/>
    <dbReference type="eggNOG" id="arCOG06049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415982_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008811">
        <property type="entry name" value="Glycosyl_hydrolases_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268:SF32">
        <property type="entry name" value="GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05691">
        <property type="entry name" value="Raffinose_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="569"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="645" mass="74033" checksum="002ACA08CEBE9265" modified="2011-06-28" version="1">MSIKMRKISLEKVDDIVSPTLSFSDGKYLEYRVHNDSFTILNFDEKIGIKSSLQFSYAEIGIEVPKGKFLALTISPIVDYSNGYKYYNELYTYGRTDAVKPGNLSYPVFREFKNFDDINKVGPCWSYSYVEGELVYPTIAVLFKEEGISNNYVTYLVNSQEGVTSSIRENKLIIEGKYTPNKVFWALFIGRSEDPYESIRAAFKEMSKCDNVKLREEKLKPSILGKLGWCSWNAFLTNISESKVLDVIKGILDRGIKLSYVLIDDGWQKLENKVMASIDPDEVKFPGGFRRTVNVLKKLGIEKVGLWHTINIYWNGYNEKVKEELGDGERTNGGYQIPHQLDRVLKVYYNFHKRVKDNGFSFVKVDNQWVIRKYSKPDEIEKAVQLSASLNGLDVMNCMSMVPECYTNYFLSNIMRTSNDYIPMWKEDAKLHLLFNAYNSLFFSNIAYPDYDMFVSYDDYALPHLIFRIFSGGPVYITDKDPSRTNVELLRKVMIEDKVLTVDFPGLVTKDILFVNPLREEKLLKLASKSNGIPVVAVVNINSMRIKDKIRAEDFPYPLNGDLMYYMVIRGEHGYLKDLELELDEMEAEILVISSKGSPIGLKEYLLPPATIKDGRTLASGTLVFLDEEIKEIKVNKGYQVNLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H9</accession>
    <name>F4B5H9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776318.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H9"/>
    <dbReference type="STRING" id="933801.Ahos_1520"/>
    <dbReference type="GeneID" id="10601020"/>
    <dbReference type="KEGG" id="aho:Ahos_1520"/>
    <dbReference type="eggNOG" id="arCOG00816">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_3_2"/>
    <dbReference type="OrthoDB" id="30861at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF6">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="79" mass="8747" checksum="F0F8BF4A8843FAF7" modified="2011-06-28" version="1">MEEVKVTRNYQITIPYDVREKLGIKIGDKLVVYVEGDKIIIRKKSGNIASLNLTLGKKITDKEINEAINEAGEKIGSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J1</accession>
    <name>F4B5J1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776330.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J1"/>
    <dbReference type="STRING" id="933801.Ahos_1532"/>
    <dbReference type="GeneID" id="10601032"/>
    <dbReference type="KEGG" id="aho:Ahos_1532"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075237_0_0_2"/>
    <dbReference type="OrthoDB" id="44187at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="32246" checksum="DF1F2C1A3AC2663E" modified="2011-06-28" version="1">MINPYADGLKELKKGSLYEILANIISFIGAIILLLLLFTYYGFIISSPTTTTSISNLQLNSSLIGILAAAVIIVIIGAILSIVGIIKLRSGFNLLKNTGLDVSIGSTGATLILISLGILIVGVATVIIAVGIFIIVIAAILELIGGIMLGLGFYNLGKGLNNSTIETAGILIIISGIIDIVISVGGILEFIAFILIYTSINGILSKGIPYVQTFSQLLGVIKGNGYAYLNVYSQVEGTIISARIEGTSISSTSITPNKLSVGNNSITVNFGSVQGLIPYSNYIVSLIVQDNSGRTVLIPVNVQYQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J8</accession>
    <name>F4B5J8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1539</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776337.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J8"/>
    <dbReference type="STRING" id="933801.Ahos_1539"/>
    <dbReference type="GeneID" id="10601039"/>
    <dbReference type="KEGG" id="aho:Ahos_1539"/>
    <dbReference type="eggNOG" id="arCOG00188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_7_2"/>
    <dbReference type="OrthoDB" id="35850at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000224">
        <property type="entry name" value="Energy-coupling factor transporter ATP-binding protein EcfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF27">
        <property type="entry name" value="ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="4"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="277"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="526" mass="59295" checksum="078AA44A3DCEEE15" modified="2011-06-28" version="1">MKFVEIKDFQVTYLGRSKPSLVIDDFEIEDGESVLIVGKSGSGKSTLVNAINGVIPNMINAQETGEISVYGKDPRKQPIYETAKLVGTLLQDPETQIFNYTVVDEIAFGPENFMLPKEEIIERVNEASKLTGISHLLHRDTNTLSGGEMQRVALASILAMRPRALILDEPTSNIDPKGTAEIFSLIKNFRQERRSLIIVEHKLERVLPYVDRVVLIDRGRIVLDVPKEELIESSDLLASAGVEIPDYYLYLKKIGAKSLSKELLKGYKYVPPPRRKGEKEALYAKVKVWTKQGKTLVDAEISIKQGEIVALMGNNGAGKSTLLKAIMGMLDRKLKAEVKLVVEGKDLSKKGLAERGSYIGYLPQNFDIMFVKRKVEDEVAFAMKNRKTFSEEKLNNILQMFSLQRVRNDDPLLLSMGQRRRVAMASILASGARILLMDEPTSGQDWYHREILGREVQDLREKGYTFLIVTHDSRFVDKFCDRVLVMKDGKIVFNGTPEEAFLREDLGIYPPTEYVVTKLGTLEFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K1</accession>
    <name>F4B5K1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776340.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K1"/>
    <dbReference type="GeneID" id="10601042"/>
    <dbReference type="KEGG" id="aho:Ahos_1542"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134836_0_0_2"/>
    <dbReference type="OrthoDB" id="36908at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="120" mass="13817" checksum="36C788C311B2FD14" modified="2011-06-28" version="1">MQEFTANLYSRAKNFLEAAVYNMKGGYYDISAILFSRAVYLALVAKLLDSGVEIPWYLDFAGLFRLLKKKLKIDVESEFSELLDAIRIHDCYSTPIEINEVEIKLLNEKVKDLFVKLNVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K6</accession>
    <name>F4B5K6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved Sulfolobus plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
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</entry>
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                <person name="Shah S.A."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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</entry>
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    <accession>F4B5T9</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93304.1"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B5T9"/>
    <dbReference type="STRING" id="933801.Ahos_0416"/>
    <dbReference type="GeneID" id="71811277"/>
    <dbReference type="KEGG" id="aho:Ahos_0416"/>
    <dbReference type="eggNOG" id="arCOG09934">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_705182_0_0_2"/>
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        <property type="component" value="Chromosome"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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            <end position="36"/>
        </location>
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        <location>
            <begin position="42"/>
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        </location>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44387" checksum="DB4FF7B6EF7CBFD3" modified="2011-06-28" version="1">MGFNIFKNRDVKIYLIQHSIQPYSALSFILPAYVFLFSKNIILVGVLLSIAYLLGHLTIISGFLTDYVVNTRLYDFILSVLSGLIFLVSSLVYSNSVNFIFLLYAVLGVVYTLKGTNHRKVLRNVVNSYKDYRNIWVLTHSIRAFTSIILLYISLFIVGLKYTLLILGLYMLFVHTPLIFLINYPRGTGNAKLIFNMSFSLLKGKKCAQFYVIDNFFSNFFSGILPVFIGAFSLSFSFLTNYIIYYVAFILYDTIYLIILNKDLKGVNGRYSVLFLRGLVPSFILFSIVILSPSLYIIPIVLFSVVLTFYNVFVTQAIQFNIPKEIYATFIGLNVAFGSLFYTLGNLILPYMEITLGLKGVMVLVSIVLAIYSLIISYRFKDLFKTIKK</sequence>
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    <accession>F4B5U8</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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    </evidence>
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        <source>
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    </evidence>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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    </evidence>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <source>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    <sequence length="89" mass="9914" checksum="3E8E74B8DFA20A2E" modified="2011-06-28" version="1">MILGSVTALKDDLIIEFNNITGTISFFIQSLDKIDLVEEDLRRIVGNLNLGKIDYIELSLEIIFTGKLNLNMLGGEVVGLDLLNEKSYP</sequence>
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    <accession>F4B5Z6</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94494.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="158" mass="18008" checksum="875C1F48CFF2C3C8" modified="2011-06-28" version="1">MCNMLHMIEKQVLAKVLSVSLSDSEILNLLGKLKENDSELERECGISRKTIYNWKKGKVGEISRETKEKLVSFILNKYDKEGLMTIAEVKRYSYIQILLELMREGLNEEEKRKLEVIVKDDKVSGGEAVIPNSTPAYSDYSSNYNKTTNSSLLSLSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B636</accession>
    <name>F4B636_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775240.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B636"/>
    <dbReference type="STRING" id="933801.Ahos_0436"/>
    <dbReference type="GeneID" id="58788748"/>
    <dbReference type="KEGG" id="aho:Ahos_0436"/>
    <dbReference type="HOGENOM" id="CLU_3002933_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="56" mass="6819" checksum="9AD02D2825D38DEE" modified="2011-06-28" version="1">MTFSYAEIFSDKERFIPELLYGRENDLKKLVDLVSKQDLTLFFIKWPRRRGNRCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B678</accession>
    <name>F4B678_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B678"/>
    <dbReference type="STRING" id="933801.Ahos_0478"/>
    <dbReference type="KEGG" id="aho:Ahos_0478"/>
    <dbReference type="eggNOG" id="arCOG07271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133055_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="172" mass="18840" checksum="264B49FC457B9830" modified="2011-06-28" version="1">MNFKVILVGVIIFLIGLAGLLLLPLYFPNYSIVNNQYVKHYSTVTLPANAGKIVLTFNITKENDSIIAFIRSGNANISIINGTPPFHVVCNKQGMVSLILSPGNYSMVIVNDESTPQNVTYTYGIFNAGFITSFYNGLGIITTIMEIIAVGGIALAFISLIYELLSIKRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B693</accession>
    <name>F4B693_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B693"/>
    <dbReference type="STRING" id="933801.Ahos_0493"/>
    <dbReference type="KEGG" id="aho:Ahos_0493"/>
    <dbReference type="eggNOG" id="arCOG05899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663271_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="51115" checksum="6515D9765C574C58" modified="2011-06-28" version="1">MLKYTLVVLALLVVMPFTQILSLALPSSPPLTTNTIINEIPYYSHLKYLISIYNETTENATTKMKFIANYTANYNVISQHNGIIEVNYNISKIFTKYNLLTFNMSNITFLRPGIFNVTLLNDALCFHYPYIYPGLLYNSSTYYLYFVATKFNFPLGYVNETKVTINNSTFIAYKYHNLSTFSTINQYFYVLCNGIGYNFNYTFSYKIGNKTYNLYANFLLKCLSGNSSTQLKYLGNQLEEAFKPYVYLECTYSSLYAGLAPNTYREIFYPYIFANGYFIYRYNLLNFIAEQPVGLSYFGLSLVIGNYTDLPITFLVCNSSNIIWENEEFTKVNVTSLNILGKTYNNVAEYENISAKSFIILYFYNGTLIEEEAGNIDGNTYNVTEKVEFVGNSMIPLNITYPNYFDYTNTNLPYKAIDPSLALEVSIIITMIIVALIVLLYKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B0</accession>
    <name>F4B6B0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LAO/AO transport system ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775314.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B0"/>
    <dbReference type="STRING" id="933801.Ahos_0510"/>
    <dbReference type="GeneID" id="10599951"/>
    <dbReference type="KEGG" id="aho:Ahos_0510"/>
    <dbReference type="eggNOG" id="arCOG01226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052040">
        <property type="entry name" value="GTPase/Isobutyryl-CoA_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087:SF1">
        <property type="entry name" value="LAO_AO TRANSPORT SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43087">
        <property type="entry name" value="LYSINE/ARGININE/ORNITHINE TRANSPORT SYSTEM KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03308">
        <property type="entry name" value="MeaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="33902" checksum="4DB398111D007C1C" modified="2011-06-28" version="1">MDKLLENALKGDELSISRVLTRIEYMTDEGMKYLEELSKKAGSAHTIGITGIPGAGKSTLISSLIEEFSKRGNKKIGVIMIDPSSPISMGSFMGNRIRMQDKTMMNNVFIRSIASRGHLGGVSSEAIMLIEAMDGLGFNPIIVETVGAGQTDTEVVSSVHTITVVNVPGTGDEIQALKAGIMEIGDIYVINKAEYPEADVLYDEIKFAIDSGNWNGWKPLVLKVSALKKIGIKELVDAIETHEKYLKESGKFAKKVNERRKKMIELIIRRKIDEKINEVINKEALDNWTDLLTIVNRIYEDVKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C9</accession>
    <name>F4B6C9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1671</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="123" mass="13994" checksum="F020EE6D18C70BC8" modified="2011-06-28" version="1">MSNVNLLSASDVLIKEADELLEKGDTAQASEKYYKAAEEAIKPITKTLNIQDVLQKVKSLGRWSSTLLFEGAMSISPEMYSIWSDAWYLHIYGFHEMKLTYNEVKSSSHNIHKIIKILSDLIK</sequence>
</entry>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10600004"/>
    <dbReference type="KEGG" id="aho:Ahos_0561"/>
    <dbReference type="eggNOG" id="arCOG00891">
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        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
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        <location>
            <position position="6"/>
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        <ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:60240"/>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="243" mass="27940" checksum="0324B028BBF256A2" modified="2011-06-28" version="1">MLVDIHAHLNSDELKDRIDEILSKCNIKIINAGVDLDSDLETLELARIYKDIVYPAIGFHPEYIEKAEKEIDKILPLIDKAIAISEVGLDYYWIKDEKLRKKEIEVLSTFLEIGEKRRKPVILHIRGGMSDFLQLISSYKLTFIIHAFEGSIKIARKILDLGGYLSFPPIIVRDKLRRQIAKEVPLDKLFTETDSPFLGPEKGKINEPCNVSITLSELSIIKGLGKEEIEKQIEKNFYRVFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P3</accession>
    <name>F4B6P3_ACIHW</name>
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        <submittedName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="293" mass="33844" checksum="CD1B36E560692B42" modified="2011-06-28" version="1">MIFILGSGYAGLNAYYNIKGGAEKILLSHENKFVFYTAYLRNLIKNTKYEVSLNFVRKEDVKNVDISNLEIKTDKGEYKADKIIIALGCKRPNLEYIMERINNKNVKEEMCITAEDEYDDYLALQITLYSKIAGKNVRYHGNFLSYLGNKVANVVSELTQKYIKTCEEPNLIIEKCVPPEFTGLLSVNSYLEVKKSVYAIGDIIKGWPKLGELAMREGIYIGKKISGKLEGEFEPIYIHIVDTGREGIHIRSKVPWGINYQEVKVSKIRSLMKRFIEKYYIWRKGNMGFLYYL</sequence>
</entry>
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    <accession>F4B6Q5</accession>
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        <submittedName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005104">
        <property type="entry name" value="WHTH_HrcA_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03444">
        <property type="entry name" value="WHD_HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005063">
        <property type="entry name" value="UCP005063_CBS_MJ1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="178"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="241"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="300" mass="33296" checksum="3CE55992C8ED9CDF" modified="2011-06-28" version="1">MQNLSSTQREILLALIELYNKGKKMIKSKEVADMIGKDEGTVRNIILGLKVLGLVESKPGPNGGYIPTLKAYEFIKNPSISPILDKLSLYRGNVETDIKIDNIELLDVTNPSGNKVILKVTGDLRKIRPGDPVRLGPTPYSRLVIDGVVINADDSRKEVLIDVKRMISVPKEKVKNIIGRRLITLKPDMTLKEASMILYKEGIRGAPVLGEGEKVLGIITTADIIKAFFEGKYDAKVSDYMKTDVITIRDEEDVLEAIRKMVIYNVGRLLVVDSMQRVIGIVTRTDILKSIAGLELLWGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R1</accession>
    <name>F4B6R1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein Ahos_0583</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0583</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CinA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775387.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R1"/>
    <dbReference type="STRING" id="933801.Ahos_0583"/>
    <dbReference type="GeneID" id="10600027"/>
    <dbReference type="KEGG" id="aho:Ahos_0583"/>
    <dbReference type="eggNOG" id="arCOG00215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_5_2"/>
    <dbReference type="OrthoDB" id="372037at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00226_A">
        <property type="entry name" value="CinA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050101">
        <property type="entry name" value="CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023055">
        <property type="entry name" value="CinA_Arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002291">
        <property type="entry name" value="PRK01215.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE AMIDOHYDROLASE PNCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13939:SF0">
        <property type="entry name" value="NMN AMIDOHYDROLASE-LIKE PROTEIN YFAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="235"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="30321" checksum="F53096E26873B1A9" modified="2011-06-28" version="1">MRTYLAEILTIGNEILSGRTVNTNASHIARRLTSLGFTVRRITVVMDELDEISQGFKEATQRSPKVIISTGGLGPTYDDKTAEGLSLALGRKLVINQKALEEIENKYRQLGLQITEERRKMAMMPEGSIAIENSKGIAPGIYIEYNGIEILATPGVPREMEDILETFIKKYMKTRPEVKYVEETIYADKVMESALAPYVKQLVKKYDLYIKTHPKGYELSNPSLEIQIAGSSKDEEEIRKRINECIKELEEYIKNLGGVIKNSQQENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U7</accession>
    <name>F4B6U7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776555.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U7"/>
    <dbReference type="STRING" id="933801.Ahos_1763"/>
    <dbReference type="GeneID" id="10601263"/>
    <dbReference type="KEGG" id="aho:Ahos_1763"/>
    <dbReference type="eggNOG" id="arCOG09819">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718291_0_0_2"/>
    <dbReference type="OrthoDB" id="41253at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17841" checksum="6ADEDEECA0CB53D0" modified="2011-06-28" version="1">MYVLFKGTLTNFLFSLDDYKTRKSKLIQKYPQGSFIWGFNRSSKLLENQVRAFLYLNKSESRLKGGIVLEGEIIDIAELSEKYWPEGEWKYYVTLKIIYIPKSVLSTTDTTRWKIIDLDKLKEIGVKILPGIQKIDNELGRRIEKLLGEIDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W0</accession>
    <name>F4B6W0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776568.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6W0"/>
    <dbReference type="STRING" id="933801.Ahos_1777"/>
    <dbReference type="GeneID" id="10601277"/>
    <dbReference type="KEGG" id="aho:Ahos_1777"/>
    <dbReference type="HOGENOM" id="CLU_2613392_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="9018" checksum="AEB832DBBFD301D6" modified="2011-06-28" version="1">MQPYNESVNSTAIAIAEEQLSKEVQQLGTGGPPFPVPPPPWYIQYLPEILLTSFAILGVSLFFMYRRYRIKRVFRNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X9</accession>
    <name>F4B6X9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1796</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776587.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X9"/>
    <dbReference type="GeneID" id="71811315"/>
    <dbReference type="KEGG" id="aho:Ahos_1796"/>
    <dbReference type="eggNOG" id="arCOG07304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3178367_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="46" mass="5269" checksum="B8CF433BEC3D0805" modified="2011-06-28" version="1">MKVLIKDVDEKLYRMLKARASIEGISIGETVNEAIKLLLLNKELIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z6</accession>
    <name>F4B6Z6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775395.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z6"/>
    <dbReference type="STRING" id="933801.Ahos_0591"/>
    <dbReference type="GeneID" id="10600036"/>
    <dbReference type="KEGG" id="aho:Ahos_0591"/>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_0_2"/>
    <dbReference type="OrthoDB" id="8895at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="392" mass="45829" checksum="6ADD58FFFF4EE23F" modified="2011-06-28" version="1">MKKIYDEIHGYITLGDAEIEIVDSPEFQRLRRVKQTSLAYIVYPGATHTRFSHSLGTFYLASKVGEKLYNSGEINAEELQDLKMASLLHDIGQFPFSHAIEGYYLPQGFGNKELREYILEKSEIKDILEKYGFSISRILDIYHGNSLLSSIIDGEVDVDRIDYLMRDSRHTGVQLGKLDLDRLIDTISYTQSGITVEDKGIISLENFYISRLHMYQAVYYHKTILGYELFLRNLYEYMINYCNKDLKDSSNIREMVKEGLFPYWDDEWIISSLYNCLTEDIPLSIIQKIKNFMDRKGPKVVYDEVSYKEEKGYLEDIRNQLEKVGIPSDSIYPFEEKISIIDKNKIKILSKGREKKLKDYSATLLHEIPDFILIRRVYVDYEYAKKAREVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B701</accession>
    <name>F4B701_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trm112 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775400.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B701"/>
    <dbReference type="STRING" id="933801.Ahos_0596"/>
    <dbReference type="GeneID" id="10600043"/>
    <dbReference type="KEGG" id="aho:Ahos_0596"/>
    <dbReference type="eggNOG" id="arCOG04124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155659_4_3_2"/>
    <dbReference type="OrthoDB" id="6467at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15292" checksum="A280C388AC80BD29" modified="2011-06-28" version="1">MKYRLMDILACPMCKHFPLQLIVISQKKVERTTDEKKPLCELYCAYKNSEVKNVENPPCDECITYEIEEGVLYCPECKRWYPIIDEIPRMLPDKLRKKEEDLKFLEKNKDKIPSYIIENGVPFNLSSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B721</accession>
    <name>F4B721_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BFD domain protein (2Fe-2S)-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0616</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775420.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B721"/>
    <dbReference type="STRING" id="933801.Ahos_0616"/>
    <dbReference type="GeneID" id="10600063"/>
    <dbReference type="KEGG" id="aho:Ahos_0616"/>
    <dbReference type="eggNOG" id="arCOG01299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_564558_0_0_2"/>
    <dbReference type="OrthoDB" id="36306at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="entry name" value="BFD-like [2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051691">
        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949:SF3">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFD-like [2Fe-2S]-binding" evidence="2">
        <location>
            <begin position="400"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="466" mass="53980" checksum="D418EAF31B8094AB" modified="2011-06-28" version="1">MKYIKSKKHKRYRFPKDCEEGLPYTEVIEDGKRIKVCENFEKYFHYGNHFSINFNSSIIHSKFLRSQFILNNIPRFLNLPEYYNESREIKVESNEKIDEIIIGGGISGLSALENCKNCILISPNFDDEIFLDPLCKDCSLKDKIKSIIKNNSNKIVDGRFIGRFNEGLVFRLKDKYLVIRKPAKVILASGSRFLPPVFPGNDLPGIISRRMYLKFLNLFKNVVVIGSTDDAIRTALISKSKIILKKGTENFSKKYLELAENEGIDILKVRELKASRKGKKIVVYHDYGKEIVDAIVFAIVRQPRIEIANNLGIEYNYYYKLHIYLPIHNLNGKVNDNYYVTGGMRGIGNYELSFLSGKIIFNEAIDEFYTELKETYLYHYYSEGEEESSSISPYFFGNGYVCECEDIKLKDVEEQYKKGYRTVEEIKRTLGISTGYCQGKICSFLVGDYLNSNKLITFRSPLYSMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B727</accession>
    <name>F4B727_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="3 12">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_0622</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="3 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B727"/>
    <dbReference type="STRING" id="933801.Ahos_0622"/>
    <dbReference type="KEGG" id="aho:Ahos_0622"/>
    <dbReference type="eggNOG" id="arCOG04300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00384">
        <property type="entry name" value="ALAD_PBGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000019">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="317" mass="35437" checksum="1EC94CE0F84B3832" modified="2011-06-28" version="1">MIAETNLTEKNLILPIFIKEGILEEEEIKSMPGVFRYPPNDKLIKFVESSYDNGIRNVILFGIPKYKDDIASSAYDKNGVIQASVRLLKDTFGDKLIVITDECTDEYTSHGHCGIVKYNSKGEYFVDNDESLKIHAKIALSQAEAGADIIAPSSMMDGVVGAIRKALDENGYTDTLIMSYSVKYASTFYSPFRDAAYSKPAFGDRKSYQMDPRNAYEALKEAKLDIEEGADILMVKPAHTYLDVIRLVKDNFPDYPLAAYHVSGEYSMIKAAALNGWIDERIAVLEITTAIRRAGADLILTYYANDIAKWLKEGLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B729</accession>
    <name>F4B729_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0624</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775428.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B729"/>
    <dbReference type="STRING" id="933801.Ahos_0624"/>
    <dbReference type="GeneID" id="10600071"/>
    <dbReference type="KEGG" id="aho:Ahos_0624"/>
    <dbReference type="eggNOG" id="arCOG01044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_8_2_2"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="25018" checksum="099D745C617500E2" modified="2011-06-28" version="1">MSNLPYFPVILDLEKFRILVIGGGKVGSKRALKFHKYSKNVTVVSENFSEDLLNSPIEKIKMNAENLPEDFLKRFDIIVTATNNRELNSKLCNLAKKLGKLCNNPTNPEDSNFIVPIFYSDENYEIAVTTYGKSSLVSEFILNEILDGLSQKKDFVNLLTNAMAKVKEIAKKKINDPSLRFALYYKIFYDKLFSDFLINGKYNEAINRAEEIMNEYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B742</accession>
    <name>F4B742_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription termination factor FttA</fullName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fttA</name>
        <name evidence="15" type="ordered locus">Ahos_0638</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions, which are required for nuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. FttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775441.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B742"/>
    <dbReference type="STRING" id="933801.Ahos_0638"/>
    <dbReference type="GeneID" id="10600086"/>
    <dbReference type="KEGG" id="aho:Ahos_0638"/>
    <dbReference type="eggNOG" id="arCOG00543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OrthoDB" id="7155at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="domain" description="Metallo-beta-lactamase" evidence="13">
        <location>
            <begin position="190"/>
            <end position="405"/>
        </location>
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        <location>
            <begin position="417"/>
            <end position="539"/>
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            <begin position="1"/>
            <end position="68"/>
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        <location>
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            <end position="136"/>
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            <begin position="177"/>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="240"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="638" mass="72065" checksum="488A703E8BF0B25B" modified="2011-06-28" version="1">MTSRLEILGAIYSGIPKDAGITRIEFEGPEIAVYVKNPNFLTNGGDIIKKIAKEIKKRIVVKADKSVRKDEKEAIEIIKNIVPKEAEITDIKFDEDLGEVLIKAKKPGLVIGKGGLIQQRIFLETFWKPEIIREPPIKSKTIENVITHIYNETEYRAKILKTFGDRIHREVLFKDRYVRITALGAFQEIGRSAILVETPESRVLLDTGVNPSVNFGERMFPKLDIDQLRLEDLDAVVITHAHLDHCGMVPYLFKYGYEGPVYTTPPTRDVMALMQLDLLDVAEKDGRPLPYSAKEVRKELLHTITLDYEEVTDIAPDIRLTFYNAGHILGSAMVHLHIGEGTHNIVYTGDFKYAKTRLLDRATSEFPKVDTLIMETTYGAQEQTNREESEKQLIDIINNTINRGGKVLIPVLAVGRGQEMMLVINNALKNKLIPEVPVYVTGLFDEVTAIHTAYPEWLSREVRDAILYRDENPFVSDQFKRIEGYREDIAQGEPSIILATSGMLNGGPAVEFFKAMAPDSKNSIVFVSYQAEGTLGRKVRDGAKEVQIIGRDGRVENIQINMEVKVVDGFSGHSDRRQLLKFLHDLTPKPKNIILDHGEYNSMMTFKRLIDNPKEKERLGIKGANIYTPAILDSLRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B777</accession>
    <name>F4B777_ACIHW</name>
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        <submittedName>
            <fullName evidence="5">IS607 family transposase TnpA2</fullName>
        </submittedName>
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        <name evidence="5" type="ordered locus">Ahos_1822</name>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
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            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B777"/>
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    <dbReference type="GeneID" id="10601322"/>
    <dbReference type="KEGG" id="aho:Ahos_1822"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="OrthoDB" id="28414at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-binding protein, excisionase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
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        <property type="entry name" value="DNA-bd_dom_put_sf"/>
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        <property type="entry name" value="IS607_transposase-like"/>
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        <property type="entry name" value="Recombinase/Transposase-rel"/>
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        <property type="entry name" value="SinI_DNA-bd"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RESOLVASE-RELATED"/>
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        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="4">
        <location>
            <begin position="58"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="212" mass="24415" checksum="8DEF46390D205711" modified="2011-06-28" version="1">MERLLRPKEACQLLSISYSTLLRWIREGKIRVVTTEGGKYRIPYSEIKKYLERREETRAIIYARVSSADQREDLERQINYLTNYATAKGYKVVEVLKDIASGLNTQRKGLLKLFKLVEGRSVDVVLITYKDRLTRFGFEYLEEFFSTMGVKIEVVFGEEPKDTQELVEDLISIITSFAGKIYGMRSHKKTLLIQGVKKLIGELSGEDSEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A1</accession>
    <name>F4B7A1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50857"/>
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    </evidence>
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    <sequence length="178" mass="20414" checksum="453819FFF0A945BD" modified="2011-06-28" version="1">MSAKHRDWEKIWFGVMLLLVIIFVAWSYESILTGSTATYRYGLPLKCGLPPKLPNGTLVIYMLAVQWSWIPQNVSEIIYLPNGTIQQVLITDAIMYVDGFPYIKVYPNQPIELILYSNNVVHAFYMQLPHGDQNWNIVPGIDSYAFFYAPATPGNYSFHCAEYCGIGHSYMYGYFDVQ</sequence>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    <sequence length="188" mass="21320" checksum="7F1D0C52AC8640E4" modified="2011-06-28" version="1">MRFLGIDTSGDHDISSPYVIGYAISDESSSIEKSNIGRAISEARAILNKPNYVFHAYHDDKKVREVLLDSLIKHGIKGGVIIILNRKFVNSFFFPLSKLIDKCSYAVYDNPIFDHVLSKTVKKSTKVTSSKSLTPLYLPLQVADYFSHIWWEIFSHGLKDFNQFEKILSITDELVVIGLKNSFTLVKT</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="3">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
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    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
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    <dbReference type="eggNOG" id="arCOG01320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_3_2_2"/>
    <dbReference type="OrthoDB" id="25735at2157"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004437">
        <property type="entry name" value="PRK05773.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF46">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="219" mass="25028" checksum="CA607E552AE873FF" modified="2011-06-28" version="1">MISKEEIRKNLEDGLPILIYDFDGREEETDMMFYAGVIDWKKIYILRKEAGGLICYATGNEEGKKLGLKFQTDILLNSEYRQLVKLPQYGDKPAFSLWVNHVNTRTGISDNDRALTILKLHEVISELKENEKDAVKRFYTEFYAPGHVPILLSRGIGERHGHTELATTLAEYVGLERSVVIAEMLDEGKSLSKEKVTQYARNMGFLLIEGKEILKEVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7K7</accession>
    <name>F4B7K7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8 9">Riboflavin synthase</fullName>
            <ecNumber evidence="4 8 9">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0725</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775528.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7K7"/>
    <dbReference type="STRING" id="933801.Ahos_0725"/>
    <dbReference type="GeneID" id="10600179"/>
    <dbReference type="KEGG" id="aho:Ahos_0725"/>
    <dbReference type="eggNOG" id="arCOG01322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682776_0_0_2"/>
    <dbReference type="OrthoDB" id="23911at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09210">
        <property type="entry name" value="Riboflavin_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006399">
        <property type="entry name" value="Ribfl_synth_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01506">
        <property type="entry name" value="ribC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015750">
        <property type="entry name" value="Ribfl_synth_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR01506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="153" mass="16697" checksum="91BD6404A316A046" modified="2011-06-28" version="1">MKYGVADTTFSRVDMGAIAIKVIKREDPDAEIIRYTVPGIKDLPVAARRLIDSGCDGVITLGWVGKTMLDKYSYLAASIGLIIVQVLTSKHVIDVTVHEDEADDEEKLKEIAIDRASKHARNLVLLVKEGKNALTPYAGKGLRQGYKDAGSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L0</accession>
    <name>F4B7L0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775531.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L0"/>
    <dbReference type="STRING" id="933801.Ahos_0728"/>
    <dbReference type="GeneID" id="10600182"/>
    <dbReference type="KEGG" id="aho:Ahos_0728"/>
    <dbReference type="eggNOG" id="arCOG00692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_1_0_2"/>
    <dbReference type="OrthoDB" id="42910at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF5">
        <property type="entry name" value="CHLOROHYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="51"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="373" mass="42022" checksum="077533D80AD3BDEE" modified="2011-06-28" version="1">MENCKVINVRKALIGEDLEVKDNVNIEICNGYITHIGNGFSSNAKSFTNEIAIPSLINSHVHMLDYAFPEIGIDKTIKELVGDPNSLKYELLSKQNEKDLINFSLNFISNSINFGVTTIADFRELGILGSKIALNIKRKLKGINYIILGRLEKNEFNDKNLVRLAEIDDGFGASSISTYSPQELMKISEIFKNKIRAAHISETLKQNLKNDLEFFMKYLRPNLIIHGTWFSDYELLILKEKNVSLVLCPRSNLWFSTGIPRIASAIKLGVNILLGTDNAAWISPNLWKEMETALLISRLQDPLSNYSKEILKAATVNARLFGTNKIDEGEKSNFIIIEGEKTGILRAYDIYSAIIKRGSEGNITYVEQPRILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S0</accession>
    <name>F4B7S0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776732.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S0"/>
    <dbReference type="STRING" id="933801.Ahos_1944"/>
    <dbReference type="GeneID" id="10601443"/>
    <dbReference type="KEGG" id="aho:Ahos_1944"/>
    <dbReference type="eggNOG" id="arCOG03738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_614847_0_0_2"/>
    <dbReference type="OrthoDB" id="34010at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="447" mass="51498" checksum="9C5ED6264425B237" modified="2011-06-28" version="1">MICNIPKVTVTTWNSNNVILVGPTYKQYLDKALNSIRNNDIASIIGQPGMGKTTILKKVQEIVKDALYMDLASKNEIEDEFWSKIDKNEIRQKVLPRLDKKKYGYSFWKRLLGVKFEDWLMRVCGKYNDPLLRLYCFDYQKDFDGMIRAISDLKEIEHLSLLIDEVRENHIPKIHRLINSGLGVPILMAVPTEVYSKITDLAIRRRLDESRISLDNALTSEDIKEIVDAYCHEISDDLFPIVLSLWNGRELNTVSSILQFMKSEVEKFEKECSNSQDVVNCVKEKLKESHSLKNPEEESKQLEKMIRDLLSSLTKEFQVTYVHPRGKRVEVKGKYITLGIFFIKDGNAYVGLVKLLNDDRTDDDEVKLLSMLEKVEHEKKEYPVEKRFIITNSQKLNVDSTVTKVELTTMEAIRILQGDSEILEEKLKEILNSFSTKTSSASAVVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U6</accession>
    <name>F4B7U6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776758.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U6"/>
    <dbReference type="STRING" id="933801.Ahos_1970"/>
    <dbReference type="GeneID" id="10601469"/>
    <dbReference type="KEGG" id="aho:Ahos_1970"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_1_0_2"/>
    <dbReference type="OrthoDB" id="42263at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="169" mass="19309" checksum="6F68DE285C72B037" modified="2011-06-28" version="1">MSEKVVWEKDVRKYGKLRIDESIDEALISLELLKQGKLRNSASKAFMAFKAFLSGLVSINNSTFSSLLDEKERKFFYKIGFTAPSNRLILYSTLLEKDFPGITNLAKEALSLHVFSYLGYDKAGEYSPITSKDDAKKWIIDFITNLAKLIIKVEEKGKEILKEVEELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y9</accession>
    <name>F4B7Y9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775582.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y9"/>
    <dbReference type="STRING" id="933801.Ahos_0780"/>
    <dbReference type="GeneID" id="10600239"/>
    <dbReference type="KEGG" id="aho:Ahos_0780"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_1_2"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000073">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000930">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001625">
        <property type="entry name" value="PRK00411.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="55"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="7">
        <location>
            <begin position="302"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="396" mass="45194" checksum="2A4442E42AD5B160" modified="2011-06-28" version="1">MSDIIDEVLSSVKNSNIFKNRQYLSPDYIPDELPHREEQIKKIASILAQVYRGERPNNIFIYGLTGTGKTAVTKFVLNKLYEKIKSFKYIYVNTRQSDTPYRILADIIESFGEKVPFTGLSTAELYRRMLKILNDEHTIIIIVLDEIDAMVEKHGDDILYRLTRINTELNNAKVSLIGITNDVKFVDNLDPRVRSSLSEEELVFPPYNAEELEDILRRRASLAFKDGVISDDIIRLCAALAARDHGDARRALDLLRVAGEIAERQGDTKITEDHIEKARIEIERDRVYEIISTLPFHSKIVLISILNGLKYKNTLTTGEVYEIYKKLTSEMGTECVTQRRVSDILNELDMVGIITATVINRGRYGKTKEVKLAVDKATIIRALKDNDEKLISLIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B810</accession>
    <name>F4B810_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo8</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase, subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo8</name>
        <name evidence="1" type="synonym">rpoG</name>
        <name evidence="2" type="ordered locus">Ahos_0801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5 6">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775603.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B810"/>
    <dbReference type="STRING" id="933801.Ahos_0801"/>
    <dbReference type="GeneID" id="10600262"/>
    <dbReference type="KEGG" id="aho:Ahos_0801"/>
    <dbReference type="eggNOG" id="arCOG04271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136588_0_0_2"/>
    <dbReference type="OrthoDB" id="34039at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00866">
        <property type="entry name" value="RNApol_arch_Rpo8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031555">
        <property type="entry name" value="RNA_pol_Rpo8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16992">
        <property type="entry name" value="RNA_pol_RpbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14881" checksum="BCF6B7F9A3A2CD6D" modified="2011-06-28" version="1">MQRINYEFNEEGTIASIQKGVLRDLYVVNIKCQDININLDVASEINIFNNNEKVNVIISRSKPSFTQNDFCGHGYIVTEKEKVNQNKKTYITIISLFGLLVKIESDSSFLNKYGFNIMDHIYFCVIKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B830</accession>
    <name>F4B830_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1982</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776769.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B830"/>
    <dbReference type="STRING" id="933801.Ahos_1982"/>
    <dbReference type="GeneID" id="10601481"/>
    <dbReference type="KEGG" id="aho:Ahos_1982"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_60_4_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050171">
        <property type="entry name" value="MFS_Transporters"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517:SF14">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23517">
        <property type="entry name" value="RESISTANCE PROTEIN MDTM, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="157"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="374" mass="41460" checksum="1B9828DB32F88AF7" modified="2011-06-28" version="1">MKEINKLALIGGSRALSGSIIWPFIGFALYEVYHFSLDFVSLFYIIQGVISVLAYIIGGYMTDFLGRVKSMVISSLSASISLLLAFLLNLPLATVFFILLQTFFNSIYNVSNTSMVGDLNKEFKGLVISFSRIRVGINAGWAVGPVIGGILFTISGFRELLLVSSIISLLPIPLLFSLPDFKGKIGISLNLSKEFILFLIPTFLTFMIMGQLGFSLLTYYNLIVGLSTFQVSLLFMVNGLLIVIFQEFIGRKLTIKMIILGMIIYAFSYLFVAFVTNFILAVIDIVFITLAEMIVSPLSQAIASSLSRDEVRGREIGIYGMVTALGRVLGSSYASFLMNYYLYSSPLILWFLISLLGFSSALLYYPLVKSDKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B857</accession>
    <name>F4B857_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Precorrin-6y C5,15-methyltransferase(Decarboxylating), CbiE subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2009</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776796.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B857"/>
    <dbReference type="STRING" id="933801.Ahos_2009"/>
    <dbReference type="GeneID" id="10601508"/>
    <dbReference type="KEGG" id="aho:Ahos_2009"/>
    <dbReference type="eggNOG" id="arCOG00650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089162_1_0_2"/>
    <dbReference type="OrthoDB" id="42238at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004455">
        <property type="entry name" value="PRK05787.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="24537" checksum="0EC9AAADAA53F06A" modified="2011-06-28" version="1">MTLYIIGVGPGDPELITLKGIEKIKSAEVITGWGSVIERFSEYVKGKKVIPLNYKEEAKLLNEVMNLAKDREVAFLNHGDPAVSDYQLLDKLKKLAKEYGVSLEIIPGVSSIIRALQMVERDLTQVIVVTLHVRGEIDYSKLKDLIKTGRDLLIIPEPYPDGVKKIALQFEKEDPILTIMEKLTYPDEKIWKFKCSEIIKGDIKFGDLTIVYVPSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B863</accession>
    <name>F4B863_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776802.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B863"/>
    <dbReference type="STRING" id="933801.Ahos_2015"/>
    <dbReference type="GeneID" id="10601514"/>
    <dbReference type="KEGG" id="aho:Ahos_2015"/>
    <dbReference type="eggNOG" id="arCOG02586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346461_0_0_2"/>
    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22832" checksum="DD447325B67727C4" modified="2011-06-28" version="1">MNADEVNILTKRALRADIESLRKIVNFLSQYNVPIAKFAIYSIIYQFAMNNVIDLGKECETCGGKCCKAGYPVPVYDYDFKEMKKNIKNLRLEKKNGFYLLPRPCQFQKGWICTINSFKPYACLSYPFATEDEQEDLLKSYDGNGVPDFRVPEFCIAGKKVKEFMNKIMEDLRKEKGREPTPTELLEKIISLYERKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B899</accession>
    <name>F4B899_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative snRNP Sm-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0812</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775614.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B899"/>
    <dbReference type="STRING" id="933801.Ahos_0812"/>
    <dbReference type="GeneID" id="10600275"/>
    <dbReference type="KEGG" id="aho:Ahos_0812"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_1_2"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01731">
        <property type="entry name" value="archaeal_Sm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022901">
        <property type="entry name" value="snRNP_Sm-like_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF5">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="4">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="76" mass="8185" checksum="D9D9B655B4055C55" modified="2011-06-28" version="1">MAETAHKVLAESLGSLVLVKLKGNKEVRGLLKSYDQHMNLVLSDSEEIQSDGSGKKIGTIVIRGDNVILISPIQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A1</accession>
    <name>F4B8A1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="7">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Spermidine synthase</fullName>
            <shortName evidence="7">SPDS</shortName>
            <shortName evidence="7">SPDSY</shortName>
            <ecNumber evidence="7">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">speE</name>
        <name evidence="10" type="ordered locus">Ahos_0814</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = thermospermine + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:59903"/>
            <dbReference type="EC" id="2.5.1.79"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775616.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A1"/>
    <dbReference type="STRING" id="933801.Ahos_0814"/>
    <dbReference type="GeneID" id="10600277"/>
    <dbReference type="KEGG" id="aho:Ahos_0814"/>
    <dbReference type="eggNOG" id="arCOG00050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_0_1_2"/>
    <dbReference type="OrthoDB" id="10538at2157"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010487">
        <property type="term" value="F:thermospermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000088">
        <property type="entry name" value="Polyamine aminopropyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037959">
        <property type="entry name" value="MFS_SpdSyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43317:SF1">
        <property type="entry name" value="THERMOSPERMINE SYNTHASE ACAULIS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="9">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="142"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="304" mass="34954" checksum="9EEA6280B32FFC2E" modified="2011-06-28" version="1">MSQFGWSWHLEWQSPCEFHGHLINRVIVDLKTQYQRVMVAELARFGKVLIIDGKIQSSVADEYIYHETLVHPLMLSVENPENVLILGGGEGATLREVLKHKIVKKATMVDIDQAVIDFAKQYLTEWHRGSFDDKRSRIIISDAKDYIEKTTESYDAIILDLTDPIKGNTSYKLYTKEFYEKIKSRLNKGGAMVTQATSPSFSLCVFSTIFNTLRQVFKLVSASIVYVPAFDGLWGFVYASDYVNPISLTKEQVNKRIKDRIEGELRFYDGETHSMIFNMPKDIRQYLANEKRVSTEENPIYVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E4</accession>
    <name>F4B8E4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl5</name>
        <name evidence="11" type="ordered locus">Ahos_0857</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This is 1 of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775659.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E4"/>
    <dbReference type="STRING" id="933801.Ahos_0857"/>
    <dbReference type="GeneID" id="10600325"/>
    <dbReference type="KEGG" id="aho:Ahos_0857"/>
    <dbReference type="eggNOG" id="arCOG04092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="OrthoDB" id="372044at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_uL5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_uL5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="10">
        <location>
            <begin position="70"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="178" mass="20050" checksum="3F4BF3F5544C1C7D" modified="2011-06-28" version="1">MSQETVQVKKENPMRRIKIAKVTVNIGLGESGERLEKAYHLLEELTGAKPVYTRAKKSIKEFGVRKGQLIGVKVTLRGQKGIEFLKRVLQAVGYKLKAESFDDYGNVSFGIAEHVIIPGTKYDPEVGVFGMDIAITLERPGYRVARRKRKTARIPKRHRISKEEAIKFLTETLGIVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E6</accession>
    <name>F4B8E6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps8</name>
        <name evidence="8" type="ordered locus">Ahos_0859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775661.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E6"/>
    <dbReference type="STRING" id="933801.Ahos_0859"/>
    <dbReference type="GeneID" id="10600327"/>
    <dbReference type="KEGG" id="aho:Ahos_0859"/>
    <dbReference type="eggNOG" id="arCOG04091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="OrthoDB" id="5670at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="15062" checksum="2C95517FFF94A2CD" modified="2011-06-28" version="1">MTVINPLANALVTIYNNEVRRNKQAIIMPSSKLIINVLRVMQKEGYIGEFEYIDDGRWGKVVVQLLGRINKCGPITPRYSLKYRDMINLPQHVRTYLPSKEIGVILVSTSKGVMTHKEAARQRLGGVALGYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K5</accession>
    <name>F4B8K5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PNGaseA glycopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8K5"/>
    <dbReference type="STRING" id="933801.Ahos_2086"/>
    <dbReference type="KEGG" id="aho:Ahos_2086"/>
    <dbReference type="eggNOG" id="arCOG05967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_432549_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="1">
        <location>
            <begin position="99"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="624" mass="70589" checksum="A10F0D023B43D749" modified="2011-06-28" version="1">MNKKKIIPILSSFLFFVLVFSLVLPSTAGTISATIIYKPIFPLNMTISKSVMPFTQDPAFYSFEAYHIVPPNVNPIVVTIAKNLIFNDTGLKPYYERVYIPPGNYSLEILNVTIREFNGTQYDRCAYVYANGIPIFWGSTQEINNSTAEADVTLFENLLQGNVTFELVIQNYYDAKIGITGLYEMNVTLYLYPGQKPQGLPNGFIPIFVNATCEHICYNYSYVILYPGMPARTQEIQIPNGTYRMMALIYEKGGGNDEFWYACEPATRPILVYYDNMLAGVVNPYETIYTGGIDLFYWKPLTSINTLSFHSPYIIDLTPFLALGNEANITVCVYNLITAYEITQSMAHDWDIAGVILLWVNESNPMICGKLNVAKHEFIDSGPMFFPVFFGECYLEDGHYYLDYSAELKFEHGIENSQVIQKGFYCARQKLSFVYEYAFLDEKFMEKAVESGMYNATLCYDVNYPITLNFDTVAVPITNPHVIPFNLTYEQNGTISLGLDYYMKWTFGDYCHEVNTTESLYSVGGFGGIIEIINSYGGAVLVKLSSNYAMTIKNLTSTWLVNGEGFKETFSAEGLQNSTVNFNGYYVYVKESFSSIGSSDPPAQYNIANEIIQIINNKILELEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K8</accession>
    <name>F4B8K8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dinitrogenase iron-molybdenum cofactor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776875.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8K8"/>
    <dbReference type="STRING" id="933801.Ahos_2089"/>
    <dbReference type="GeneID" id="10601589"/>
    <dbReference type="KEGG" id="aho:Ahos_2089"/>
    <dbReference type="eggNOG" id="arCOG02734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021615_0_0_2"/>
    <dbReference type="OrthoDB" id="25911at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051840">
        <property type="entry name" value="NifX/NifY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937:SF2">
        <property type="entry name" value="DINITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33937">
        <property type="entry name" value="IRON-MOLYBDENUM PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dinitrogenase iron-molybdenum cofactor biosynthesis" evidence="2">
        <location>
            <begin position="11"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="12801" checksum="7CBB71A07FFDAA6D" modified="2011-06-28" version="1">MRIAIPVTKGRVDGPGEGEEVLIYEIDGDEVKLVEKYENPALKATAARGAHMLKSALDKGVNAVIVAEIGPPGVRLLKGKAKIFLAEGLTVEEALEKLKRGELQETDKPTHDEHHHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M1</accession>
    <name>F4B8M1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776888.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M1"/>
    <dbReference type="STRING" id="933801.Ahos_2102"/>
    <dbReference type="GeneID" id="10601602"/>
    <dbReference type="KEGG" id="aho:Ahos_2102"/>
    <dbReference type="eggNOG" id="arCOG07248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079524_0_0_2"/>
    <dbReference type="OrthoDB" id="43791at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="33"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="16614" checksum="D2C3C559521CE10D" modified="2011-06-28" version="1">MSNEKEKEEKNGKIHLIDTDILFSKKYLNFREKEYRITNITLYEFIVVIRNKYMEMMKKGHKDRAKGYLNFLKLIKNEMKNSIIDVNKDDILKAIDIIFEREINVGDAINVVIARKMNATIVSNDKDYDRVKDLVEVIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M7</accession>
    <name>F4B8M7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peroxiredoxin</fullName>
            <ecNumber evidence="7">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_2108</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776894.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M7"/>
    <dbReference type="STRING" id="933801.Ahos_2108"/>
    <dbReference type="GeneID" id="10601608"/>
    <dbReference type="KEGG" id="aho:Ahos_2108"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_2"/>
    <dbReference type="OrthoDB" id="6924at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009668">
        <property type="entry name" value="PRK13189.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009669">
        <property type="entry name" value="PRK13190.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF128">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="9">
        <location>
            <begin position="2"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="7">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="8">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="215" mass="24341" checksum="AABE1959E3D1B253" modified="2011-06-28" version="1">MVKVYQKFPDTQVLTTKGPIDFYKDIFGKGKWLFLYAHPADFTPVCTTEFVAFAQAQPEFEKLGVQLMGLSVDSVYSHIAWLNDIEQRYGVKINFPVIADPDKKLSRMLDLVDENSGVTVRGVFIVDPQGTIRFMAQYPIEAGRKIDEMLRITKAIIVAYKAKVATPANWEPGQDVVLGAPTTLDEAELRMKMPNAKAWYLVFKKYDELPANQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S7</accession>
    <name>F4B8S7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775713.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S7"/>
    <dbReference type="STRING" id="933801.Ahos_0911"/>
    <dbReference type="GeneID" id="10600383"/>
    <dbReference type="KEGG" id="aho:Ahos_0911"/>
    <dbReference type="eggNOG" id="arCOG01933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313175_0_0_2"/>
    <dbReference type="OrthoDB" id="34429at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="77"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="24848" checksum="EB9321E4C54AD1F4" modified="2011-06-28" version="1">MRIDVHQHLGIKWVNAKEISEYFDIILLNPAYKYSCQCCVDGFYQQYLWLKNKNENREKYRLLGIYNPKCRVPLEVELGRQYEKGIVGIVLTPEKHGYKIQDIDKVLEFAEDHKMPIFIKTTQDLTQKIQEFTHLTFVILGSYYPMEEMLYNLLKYNNVFFETSGVPESFLNKIPTDRLIYGSGYPYLPFKNMHFIDVISENALKIISIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V4</accession>
    <name>F4B8V4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0938</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775740.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V4"/>
    <dbReference type="GeneID" id="10600410"/>
    <dbReference type="KEGG" id="aho:Ahos_0938"/>
    <dbReference type="eggNOG" id="arCOG03861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056018_0_0_2"/>
    <dbReference type="OrthoDB" id="44086at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="254" mass="29411" checksum="7D9E87E6807AF5DA" modified="2011-06-28" version="1">MQYTEEVTVPIGKTYINTVLSNPYYVSGILGHITLLKVYDKVKNDYFPPSDVKEADNKFLSALVLQDEKGKVIVYQGEFIGPNVLPNYIEYYLKTNDEKIEIKLNFQLQERTNGTKINIGCEFIQRKGFFDFFSKNYGKFDEHLVKGHIIPFLNSSLTKFTVMRKIYDETLELNEALIKLRELPKTSGVIMIKSDSLDFTGYFNDNGMIGSELKYNDKLYNNAEALAELFKVRNEKVKMVIYEVPIHDIRLTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B922</accession>
    <name>F4B922_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_2174</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776960.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B922"/>
    <dbReference type="STRING" id="933801.Ahos_2174"/>
    <dbReference type="GeneID" id="10601677"/>
    <dbReference type="KEGG" id="aho:Ahos_2174"/>
    <dbReference type="eggNOG" id="arCOG01180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05145">
        <property type="entry name" value="RIO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="42"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE95045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="249" mass="28793" checksum="F66E2FE842FEAE41" modified="2011-06-28" version="1">MFEKKKVEKRRKDEDLFKVVDSTLSPRTYVLLEEIASKLNIDYYLGAISSGKEAKVYPAKTVDGKYYAVKIYYISTSASKRAIQKYTFGDKRFEDIKVTNTRQLIITWARKEFKNLSIMYDSGVNVPKPVLVEENILVMDFIGENGNRAPLLKELSEDEITKDLYDEVISQLEIMVNKAKLVHGDLSEYNIMVWDKVYIIDVSQAISIESENAIDLLIRDIENMNRFFSSKGIEVYPTEDILKRLNITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B947</accession>
    <name>F4B947_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG domain protein DNA-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776985.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B947"/>
    <dbReference type="GeneID" id="70681506"/>
    <dbReference type="KEGG" id="aho:Ahos_2199"/>
    <dbReference type="eggNOG" id="arCOG03845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_206914_1_0_2"/>
    <dbReference type="OrthoDB" id="40286at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="3"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="58" mass="6787" checksum="50022BD82D5DD7A9" modified="2011-06-28" version="1">MRVVTFKAEEDLLELLDRYAIKYGLNRSEAIRKAIENLVKDEVNKETVPVARVEKIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B948</accession>
    <name>F4B948_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-dependent hydrolase, beta-lactamase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776986.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B948"/>
    <dbReference type="STRING" id="933801.Ahos_2200"/>
    <dbReference type="GeneID" id="10601704"/>
    <dbReference type="KEGG" id="aho:Ahos_2200"/>
    <dbReference type="eggNOG" id="arCOG00497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_3_0_2"/>
    <dbReference type="OrthoDB" id="28313at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42967:SF1">
        <property type="entry name" value="MBL FOLD METALLO-HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42967">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13483">
        <property type="entry name" value="Lactamase_B_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="206" mass="23797" checksum="0BDCB0A51C0B6EBF" modified="2011-06-28" version="1">MIEYFGHSMFLIDRKIVIDPHDGGSIGLPKPNIDKVDLVLITHDHYDHNAYEILSYNDMKMKYYGYINYQNYKITGYKAYHDKEKGRRRGETAIYRIEDSEGNVIVHLGDLGEYPLKEDLIKEISSPTVLMIPVGGLITIDYKEAVSLVKDLKPQIVIPMHYWVRGHLMPLDPIDNFIKELGWKVIEGKKGIEKIEDKEAIYLLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M0</accession>
    <name>F4B9M0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="3 13">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_1052</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="3 13"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M0"/>
    <dbReference type="STRING" id="933801.Ahos_1052"/>
    <dbReference type="KEGG" id="aho:Ahos_1052"/>
    <dbReference type="eggNOG" id="arCOG00790">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106570_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8 13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PD-(D/E)XK endonuclease-like" evidence="14">
        <location>
            <begin position="31"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE93936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="158" mass="18057" checksum="E088EA2435A7AF7B" modified="2011-06-28" version="1">MAIAEVYNPSTLMGELIHMGLETFNEIEGYKVTSEVEGEKEVNLGEKVVKIKGRADIILQKDEEKIIVEIKSARGDKGLPHRHHLMQLQAYLWLFGAKKGILFYVTPERFTEFTVDKPLDEATIIKLVQENISLSPSPRFAWECEYCVFSIVCPNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R5</accession>
    <name>F4B9R5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF309 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775897.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R5"/>
    <dbReference type="STRING" id="933801.Ahos_1097"/>
    <dbReference type="GeneID" id="10600571"/>
    <dbReference type="KEGG" id="aho:Ahos_1097"/>
    <dbReference type="eggNOG" id="arCOG03705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021601_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.10">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005500">
        <property type="entry name" value="DUF309"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023203">
        <property type="entry name" value="TTHA0068_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03745">
        <property type="entry name" value="DUF309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140663">
        <property type="entry name" value="TTHA0068-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="17285" checksum="C0C7978AFBB9A3FE" modified="2011-06-28" version="1">MRKRHILLYPPTIEVKNLHKINIVDIRRCKYTEIDIIDDPQKVIKILGNPIKIINVNEEKESFPNLFFNCRFWEAHEVLEKIWITEMNEKRKKYLQALILLCASMIHYLKGHEKVSDDLLNKALSLISELPEDLLPLFYISLTLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X0</accession>
    <name>F4B9X0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nep1</name>
        <name evidence="10" type="ordered locus">Ahos_2315</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:13933"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777101.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X0"/>
    <dbReference type="STRING" id="933801.Ahos_2315"/>
    <dbReference type="GeneID" id="10601823"/>
    <dbReference type="KEGG" id="aho:Ahos_2315"/>
    <dbReference type="eggNOG" id="arCOG04122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055846_1_3_2"/>
    <dbReference type="OrthoDB" id="7612at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003203">
        <property type="entry name" value="PRK04171.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="192"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="9">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-xx" evidence="9">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="9">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="9">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE95186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="216" mass="24971" checksum="7948CB9BD4EE3F7D" modified="2011-06-28" version="1">MKLNFILLDSALELVPKEILNDPSVIKNAERRGKKPQDTILDISLHYHAMRKLKDWQKRGRPDIVHMAMIMILSEKEILGDFYIHTYDSRIIKVEKDMNPPKNYNRFIGLMEQLLKFGQIPPNSSRPLMKILNVKLSDIVKKYKPVLLSEDGSKIDPMHLCNENFLLGIGAFQHGDFSEEVKGIFTEQYSISKRALETQQVVCRILSSCNYLLGWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y4</accession>
    <name>F4B9Y4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777115.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y4"/>
    <dbReference type="STRING" id="933801.Ahos_2329"/>
    <dbReference type="GeneID" id="10601837"/>
    <dbReference type="KEGG" id="aho:Ahos_2329"/>
    <dbReference type="eggNOG" id="arCOG04479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162441_0_0_2"/>
    <dbReference type="OrthoDB" id="30924at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038767">
        <property type="entry name" value="PF0610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049159">
        <property type="entry name" value="PF0610-like_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057022">
        <property type="entry name" value="PF0610-like_Zn_ribbon_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21476">
        <property type="entry name" value="PF0610-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23470">
        <property type="entry name" value="Zn_ribbon_PF0610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PF0610-like winged HTH N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="PF0610-like rubredoxin-like zinc beta-ribbon C-terminal" evidence="2">
        <location>
            <begin position="62"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="12609" checksum="25768B55C7C2E231" modified="2011-06-28" version="1">MEYDFLTTREKIFYLLMYADEPLTAKQIMKILGIKKEKEVYSHIYHLAKSSKRKDYIIIMFPPECEDCGYVFRLEIPKKPSKCPVCKSERIKPPSFLIRKKGKINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA29</accession>
    <name>F4BA29_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775942.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA29"/>
    <dbReference type="STRING" id="933801.Ahos_1142"/>
    <dbReference type="GeneID" id="10600616"/>
    <dbReference type="KEGG" id="aho:Ahos_1142"/>
    <dbReference type="eggNOG" id="arCOG01845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_2_2_2"/>
    <dbReference type="OrthoDB" id="371709at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="14647" checksum="5DEEBDF11B4D814D" modified="2011-06-28" version="1">MSQINKEEWKQKIMEALTQVFDPEIPVDIVNLGLIYDLQISDEGDVYIKLGLTAPGCPVVDDLVYTVEQVIKETVPAKKVDVDIDLETPWSPLRMTPEGREKFKKLYGYDIVEMWVQTYGLPAQEQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA34</accession>
    <name>F4BA34_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="11">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">ImGP synthase subunit HisF</fullName>
            <shortName evidence="11">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hisF</name>
        <name evidence="13" type="ordered locus">Ahos_1147</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775947.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA34"/>
    <dbReference type="STRING" id="933801.Ahos_1147"/>
    <dbReference type="GeneID" id="10600621"/>
    <dbReference type="KEGG" id="aho:Ahos_1147"/>
    <dbReference type="eggNOG" id="arCOG00617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_2"/>
    <dbReference type="OrthoDB" id="6261at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000006">
        <property type="entry name" value="Imidazole glycerol phosphate synthase subunit HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF2">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="251" mass="26792" checksum="0616C5066497126E" modified="2011-06-28" version="1">MTTKRIIACLDVKNGRVVKGVNFLNLKDKGDPVELAAKYEEEGADEIVFLDISATIEGRRTLLDVVRNTASVLSIPLTVGGGIRSLEDVSRILSSGADKVSINTAAVENKQIITQASEEFGAQAVVVAIDAKIEGNSWIVYTKSGTFRTGLEAISWAKEVESLGAGEILLTSIDKDGTRSGYDIKLTRAVAEAVNIPVIASGGAGKPEHFFEVLTEGKADAALAAGIFHDGVIRIKDLKYYLKTKGVDVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA37</accession>
    <name>F4BA37_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18 19">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="18">ATP-PRT</shortName>
            <shortName evidence="18">ATP-PRTase</shortName>
            <ecNumber evidence="6 18 19">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">hisG</name>
        <name evidence="22" type="ordered locus">Ahos_1150</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 18">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="18">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="6 18 19"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA37"/>
    <dbReference type="STRING" id="933801.Ahos_1150"/>
    <dbReference type="KEGG" id="aho:Ahos_1150"/>
    <dbReference type="eggNOG" id="arCOG02208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13594">
        <property type="entry name" value="PBP2_HisGL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000002">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000008">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP-PRT_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF10">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 18 22" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16 18" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP phosphoribosyltransferase catalytic" evidence="20">
        <location>
            <begin position="60"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine biosynthesis HisG C-terminal" evidence="21">
        <location>
            <begin position="224"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="NCBIfam" id="TIGR00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AEE94034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="299" mass="33169" checksum="4A3A9D8230C4F5A5" modified="2011-06-28" version="1">MRFWLKLWVRFLKVAIPNKGRLQQPTLQFLTQVGIKPLASDERALMIPTSWDGVQLVMMRTEDIPNLVEAGAAEIGITGHDYVTESKADVEELIKLDFGKAKIVLAVPQNWDVENVEDLRRKKNGIRIATKYYNIAKEYLDKVDIDAKIVKISGAAEVMPSLGAADAIIDVVSTGTTLKLHGLKPIDEILQTYAVVIGNKYWMKSEEAERVNLVLTMMKGVISARGRKMIFMNVDDSKLEDVISSLPAMLSPAISRLSKTNAWEVITVAEESQLPEVIAKAKAAGARDIVVVNIEKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA40</accession>
    <name>F4BA40_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="8">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">leuS</name>
        <name evidence="11" type="ordered locus">Ahos_1153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA40"/>
    <dbReference type="STRING" id="933801.Ahos_1153"/>
    <dbReference type="KEGG" id="aho:Ahos_1153"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.20">
        <property type="entry name" value="Class I aminoacyl-tRNA synthetases (RS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="9">
        <location>
            <begin position="1"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="10">
        <location>
            <begin position="633"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="560"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="881" mass="100974" checksum="7587966FAA871209" modified="2011-06-28" version="1">MKGYNVLFPMGFHYTGTPIITMADDVAKGEKEVIDIFKNIYEIPPEDIPKLSDPLFMANYFKEDIKKAMKELGLSIDWRREFTTIDPEFSSFIVWQFNKLQQQGYIVKDTHPVGWCPVHHLPVGMHDTKGDVEPDIGEYVLIYFESEKGILPVATLRPETIFGVVAVWVNPKVTYAITEIDGKRMIVTERAAFKLSFQIDNVKVLDKISGSDLIKLTAINPITGQSVLVLPGEFVDPDMATGVVMSVPAHAPFDYYYLKKVSPNTQIIPVIKVEGYGEFPAAEVVEKEKPKNDADLKKLTELVYRTEYNKGKMRDDVLLRAKPEYREELKGIVGLPVPEARKIITDFIINHGLGRKILEIMNRPVYCRCGNEVVVKILKDQWFLDYGNPEWKAKAKRLLSMMRIVPEEARKDFEYALDWLQKRACARTRGLGTPLPWDKKWIIESLSDSTIYMAYYTIAHKIKKYNLHASQLTFEFWDYVMLGKGNVEEVSKATNIPVEVLQDLRNEFTYWYPLDMRHSGSDLIPNHLSFFIYNHAAIFPENLWPKGIAVNGLVLYEGKKMSKSLRNIIPLRKAIRIYSPDVIRITLAATADMGSETNFTETVAKSVTDNLKKFYDMITTIKGLDSKEFGVPEKWMLSRLYSTIKEVTPMMDEMRFREALNEILFGLSSDINDYVEMTKAEGRNPNAEVLKEVLETWSKLISPFAPHIAEEVWNSLGHNTFVSLEEWPKFDESKVDIKTEVAHEYHKLIIDDVKAILNVYKGTPKLVKIYVADKSKMQLLIDALNVINSGGNMKTFISSHKPKNSEEAKELQKIFQYAQGLGDEMKKIASYGFDEEELVKEGLSYIKYKLGLEVKAERFTDEIKKKYNKDALPLRPAIIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA42</accession>
    <name>F4BA42_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="16">PP-loop domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_1155</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775955.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA42"/>
    <dbReference type="STRING" id="933801.Ahos_1155"/>
    <dbReference type="GeneID" id="10600629"/>
    <dbReference type="KEGG" id="aho:Ahos_1155"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000174">
        <property type="entry name" value="ATPase, PP-loop superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="2-thiouridine synthetase TtuA-like N-terminal LIM" evidence="15">
        <location>
            <begin position="3"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="14">
        <location>
            <begin position="49"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE94039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="319" mass="36169" checksum="D540C02C7B943E88" modified="2011-06-28" version="1">MICDVCKSREAVVYQPHTGRRLCKECFIDDIRQRVKKEAEKQGLLKANKILLAVSGGKDSLTLADTLASFIDPKRLVAFNIVEGISGYNRKEQAEKLRKYLDDLGIELISTSFKDSVGYTLDEMVSSARAKGLNISACTFCGGFRRKLINDAGRQVKADLVATGHNLDDEAQTVIVNILRGDVKRLVRLGDVPLKLSDKFVLRVKPLRKIYEWETTMYAFLKGFEFQEVECPYISARPTLRAKVRELLYLIEQQKPGSLLRIVEEFDKISELVRQHSPKEELPTCKICGEPTSYGREICKNCELMIQSGLLPQKYLRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA64</accession>
    <name>F4BA64_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar H+transporting two-sector ATPase Fsubunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA64"/>
    <dbReference type="STRING" id="933801.Ahos_1178"/>
    <dbReference type="KEGG" id="aho:Ahos_1178"/>
    <dbReference type="eggNOG" id="arCOG04102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135754_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="83" mass="9379" checksum="57963BE6A3BDE4D1" modified="2011-06-28" version="1">MMGAEGKVVEDPYSLEDEIKNVRKMEDVDLVLITKDLYDPVRERLESVISSQTKPLITVIPSPYSEAEPMDVRKLILRALGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA86</accession>
    <name>F4BA86_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774813.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA86"/>
    <dbReference type="STRING" id="933801.Ahos_0001"/>
    <dbReference type="GeneID" id="10601865"/>
    <dbReference type="KEGG" id="aho:Ahos_0001"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_1_2"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003245">
        <property type="entry name" value="ORC1-type DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="52"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="7">
        <location>
            <begin position="298"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE92896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="385" mass="43893" checksum="E65849B6C6FADC3E" modified="2011-06-28" version="1">MIRETLKGGKGEVIKNPKVFIDPISVFTEIPYREDIIKETAISIRYFVKNDVRFSNLFLGLTGTGKTFVVRYILNEIEEVKKEDEDYKNVIQAYVNCREVGGTPQAVLSVLAEKLTNDIVPKHGINLGEYIEKIKNAVEGKKAIVYLDEVDTLVKRRDGDIVLYQLLRADAEISVIMISNDINVRDYMEPRVLSSLGPSVIFKPYDAEQLKEILSKYAEYGLFRGTYNDEILAYIAAISAKEHGDARKAVNLLFRAAQLASGEGIIRKEHVDRAIVEYEQERLVEAIKSLPFHYKLALRASLDSEDVVTAHKIYVDLCNKLKQRPLSYRRFSDIISELDMFGIIKIKILNKGRAGGIRKYIEVPDREKIMKALDENLAEEIGYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T7</accession>
    <name>F4B3T7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Adenylate kinase related protein (AdkA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T7"/>
    <dbReference type="STRING" id="933801.Ahos_1241"/>
    <dbReference type="KEGG" id="aho:Ahos_1241"/>
    <dbReference type="eggNOG" id="arCOG01045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096329_1_0_2"/>
    <dbReference type="OrthoDB" id="85381at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="168" mass="19363" checksum="56947D1F1027B7FC" modified="2011-06-28" version="1">MPGSGKTLFANLLREKGFYVISMGDVLRKRYEKEAKIGERMMDFAKRIREIYGEGVVARLSMEEITPTMSRIAFEGVRSLAEVEEFKRLGNPIIIAIHSPPSLRYQRMISRMRPDDSKNIEDLRRRDLDEIRLGIGGVIALADYIIINDSTIDEFKKRAEEVILRITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W5</accession>
    <name>F4B3W5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1269</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776068.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W5"/>
    <dbReference type="STRING" id="933801.Ahos_1269"/>
    <dbReference type="GeneID" id="10600750"/>
    <dbReference type="KEGG" id="aho:Ahos_1269"/>
    <dbReference type="eggNOG" id="arCOG00857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_2"/>
    <dbReference type="OrthoDB" id="193284at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF149">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="387"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="457" mass="51616" checksum="F7A75F93A19CCC49" modified="2011-06-28" version="1">MSLATVLYEWYKKNPSKTFLGGDRSLTYQQVVDEVRRTASLISPGETVAHIMFNSTRSVINYLSVYWAGGKVVAIDPLTSAEDLKFILEDSQPSLVFTDNEVYEREKNVLKDYKVIINSTNGSYEQTSPYEYREDEVGLIYYYAGIAGRTMQVLHSAGRVELNSASLYKALGVKDISSILTVPLAHVLGNSVLGVTLEAGGFMYVLRKFDPKETISAIDKFSINYLATVPMVYDALAKEKEGNLDSLRLCISTAAPLFPSTIKAFKERFNKDIVQQYGFTEGLVITYQPPEFSQVISIGKPLPNAEVKVVKDNGEEAKIGETGELWVKAPWLMLGYKDEKETKRVFEGEWLKTGDLVSMDEKGLLYFKGVKKRMLKYKGYPIFPRDLEEILLSHPLVKEAYVYGEDAGNLGTQPVAKVIVKEHKEGLEDELLNYVNQRVAFYKRLKKVYIVDKIERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y1</accession>
    <name>F4B3Y1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS domain containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774862.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y1"/>
    <dbReference type="STRING" id="933801.Ahos_0050"/>
    <dbReference type="GeneID" id="10599490"/>
    <dbReference type="KEGG" id="aho:Ahos_0050"/>
    <dbReference type="eggNOG" id="arCOG00601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_734888_0_0_2"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04584">
        <property type="entry name" value="CBS_pair_AcuB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="entry name" value="Ribosome hibernation promotion factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036983">
        <property type="entry name" value="UCP_2CBS_MJ1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="Ribosome binding protein Y (YfiA homologue)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="67"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="133"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="209"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE92945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="376" mass="42584" checksum="6A3CEB841C36DAD1" modified="2011-06-28" version="1">MVDLEELYVEPQVVANPNDRLRDILPKIKEKNQWVVPVVLHKKVIGLLTYKDLLKRRVSLDAKVLTLSSPSITIDEKDDFSKVIAKFYTTKARAIPVVNKNKELLGMITRESVIKYLLDTNQIESNKKAREYMSSPALTIEAEESVARARWLMIRDNISRLPVTREKKLEGIVSSRDIVNALYSINEKKRSSILTEEERIMAMPVKEIMTSPVISVKGSDSLKSVAELLLKKNISGAPVLEGDFIEGVISTIDIIKSLESKYNLTMPIEAKLTSGLKKEEIKAEIDGVIERYLSKLEKMTDIINFKVSFKESAKSQDKTLYKTTVTVTTKEGNFIASETDWDPIVAVKKAVEKVENNLIKKLKKIDKSKKVTKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y4</accession>
    <name>F4B3Y4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y4"/>
    <dbReference type="STRING" id="933801.Ahos_0053"/>
    <dbReference type="KEGG" id="aho:Ahos_0053"/>
    <dbReference type="eggNOG" id="arCOG05905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335106_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="183" mass="21744" checksum="B035714326E361EF" modified="2011-06-28" version="1">MLIPDEKNGKIVVYVDNWLVKAFEGNKEITDLQFYVDNEERILINRIREINPSVNILPALAYPNKERKIRLNQLLGKVFEDYVYSILSRKYSVERNKEIYSTIKGVSLHNKPDFIVENKIAIEAKVRESNLEQVTQYAKKFKYGAIVFPYSGKCRVPKNWICLFYLMKDYNRLYKWIDLHLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y5</accession>
    <name>F4B3Y5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0054"/>
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    <dbReference type="KEGG" id="aho:Ahos_0054"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="24905" checksum="BAC05D3D351C7F69" modified="2011-06-28" version="1">MFVTLTDIAWAIVLTIWVMIVTLYISKYVARKTTIYVARKTIHILGGGLVAVVSPFVFSSPLLPIILSYLLTAYLIFHRERQPLNWFQDKENRGEVWFTFSFGTILLLSWILIRNFWDPGSRDIYVALLPLFYMSFGDGVTGIIRNYVYKRRVKGWWGSLGMFIVSSLLGYYFLSIPGLISGILATIVERIGKVDDNILVPFVPFIFLYITVVLLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y8</accession>
    <name>F4B3Y8_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774869.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y8"/>
    <dbReference type="STRING" id="933801.Ahos_0057"/>
    <dbReference type="GeneID" id="10599498"/>
    <dbReference type="KEGG" id="aho:Ahos_0057"/>
    <dbReference type="eggNOG" id="arCOG00755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_851558_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="122"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="326" mass="37043" checksum="43D4D38D2066A264" modified="2011-06-28" version="1">MKVTIIGSGIAGSSLYYMFKKAGHNVKVIDPRVRRIFPSLIHSLLLVDKDVYLSKLALDFYNEFNIETKEFPSFTIGKIDEKLIKLWEEYGVKIEEKIIFNSKGIYAVGGDRLVYIKRMIDGVPIIKEKASVIKKGNSAIAKVNDKYLESDIIILAAGPWNNKIFSVPTKSYYCWASLTLTQDSKLDRMFVYDYELNFYSRPFLGLGIRTAIIGDGETIEAKPCQRIKVDPEEVMTRARKRLGKLRVIYTSGEFCEGTPDMRPAYGRLLDNLYFIGGFDGYGAEVGPGVARLLFNFITKGEEDKDYIVDRFNGVKDFKIGKEPHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y9</accession>
    <name>F4B3Y9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774870.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y9"/>
    <dbReference type="STRING" id="933801.Ahos_0058"/>
    <dbReference type="GeneID" id="10599499"/>
    <dbReference type="KEGG" id="aho:Ahos_0058"/>
    <dbReference type="eggNOG" id="arCOG01830">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1025340_0_0_2"/>
    <dbReference type="OrthoDB" id="34551at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="30042" checksum="6B8F5112B01EFB46" modified="2011-06-28" version="1">MITEGYFVKYKGVIWAVKGCYHPEGYVVAVPRKLGEKKIKKMSESIQLVKEKFPQLLKYVDEIGFEVPLLSLSEVEVLDPFKVSIFQFNDFINNFLDVGITGSYLYEGKGNDLDLISFNSKNYYILMDMRKRGITSPLVEVKDNEIEGLSKQDFLRLKVKRVLEGKYKGIPYTFKIVECEDFGKVIRKSKFGGIVKIDKALKPFSLPVKYLTEEGYILTSFRTRYTELEEGLRLYIKGTLLSRENFEDLDLDIAQVVKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B460</accession>
    <name>F4B460_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps19e</name>
        <name evidence="5" type="ordered locus">Ahos_1285</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776084.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B460"/>
    <dbReference type="STRING" id="933801.Ahos_1285"/>
    <dbReference type="GeneID" id="10600766"/>
    <dbReference type="KEGG" id="aho:Ahos_1285"/>
    <dbReference type="eggNOG" id="arCOG01344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="OrthoDB" id="371836at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_eS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_eS19_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006811">
        <property type="entry name" value="PRK09333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17405" checksum="F14A0E67C7DE0FD0" modified="2011-06-28" version="1">MITADMVPPNLLIDKLANYIKENVKEVQPPEWAFFAKTASYKERVPDDLENWWYIRAASLLRKLYKEAFGISTSRTIYSGLKRRGTKPPHTVKAPGHANRLIFQQLEKAGLVIKTKNGRSLSPKGRSLLDKLSYEIFKGLVENNPSLKKYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B467</accession>
    <name>F4B467_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="8">SRP receptor</shortName>
            <ecNumber evidence="8">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsY</name>
        <name evidence="10" type="ordered locus">Ahos_1292</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776091.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B467"/>
    <dbReference type="STRING" id="933801.Ahos_1292"/>
    <dbReference type="GeneID" id="10600773"/>
    <dbReference type="KEGG" id="aho:Ahos_1292"/>
    <dbReference type="eggNOG" id="arCOG01227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17874">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000053">
        <property type="entry name" value="Signal recognition particle receptor FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="9">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="120"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="202"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="260"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="308" mass="34471" checksum="87F915B913032E59" modified="2011-06-28" version="1">MEEKKEEPTKEKGSIGSKLNIFNIFRYREIKEDDIDDIIEELRTELLESDVSLEVTDKILEDLKSSLIGKKVNRSEDLDELVKKSLKNSITEILEKNKFERNLIDEIKKSPKPYVIMFFGVNGVGKTTTIAKVAYMLKKAGLKVIVSASDTFRAAAQEQLAIHCQKLEIPLVKGKYGGDPASVAFDAIQAAKSRGIDVVLIDTAGRMHVDKDLTEELKRIVRISKPNLKLLVIDALAGNDALEQAKYFENVVDYDGVILTKVDADAKGGVVLSLAYELKKPVIFLGTGQNYDDLIPFSVDWFIERIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B477</accession>
    <name>F4B477_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="4">HMG-CoA synthase</shortName>
            <shortName evidence="4">HMGCS</shortName>
            <ecNumber evidence="4">2.3.3.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1302</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds that are building blocks of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with acetoacetyl-CoA thiolase that catalyzes the precedent step in the pathway and with a DUF35 protein. The acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the thiolase-like superfamily. Archaeal HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776101.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B477"/>
    <dbReference type="STRING" id="933801.Ahos_1302"/>
    <dbReference type="GeneID" id="10600784"/>
    <dbReference type="KEGG" id="aho:Ahos_1302"/>
    <dbReference type="eggNOG" id="arCOG01767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_7_0_2"/>
    <dbReference type="OrthoDB" id="5812at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="HMG_CoA_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="HMG_CoA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003274">
        <property type="entry name" value="PRK04262.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="5">
        <location>
            <begin position="221"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
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        <location>
            <position position="112"/>
        </location>
        <ligand>
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        <location>
            <position position="153"/>
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        <location>
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        <location>
            <position position="240"/>
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        <ligand>
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    </feature>
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        <location>
            <position position="267"/>
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        <ligand>
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            <position position="297"/>
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        <ligand>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="348" mass="37647" checksum="F6CCFA9484C65709" modified="2011-06-28" version="1">MRSGIVGWGSYIPRYRINVNDIAKVWGKEATTPKVLGLTEKSVPAPDEDSTTMAIEASRNALNRARINPEEIGLVLFGSESKVYAVKPTAAVLIDALGISHYSLSADMEFACRAASVGLGLALSMVESGKIKYGMVVGSDTAQSNPGDILELSSAAAAVSFIVGKSDESAAVLEGFASFNTDTPDFWRRDGMPYPLHGEAFTGEPAYFAHIISAVEKLFQDYGYKVSDFDYFVFHQPNGKFPIQAAKKLGVPLEKVKPGLVSPYIGNPYNASALLGFAKVLDIARPGQRILVAPFGSGAGADAFSFVTTENILEKQKLAHTTDYYISNKIIVSYAEYAKFTNKYKVYE</sequence>
</entry>
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    <accession>F4B493</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <source>
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        </source>
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    </dbReference>
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            <begin position="20"/>
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            <begin position="51"/>
            <end position="71"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="21723" checksum="50422BD2C459B889" modified="2011-06-28" version="1">MNLTKGRVMEGRGYLFLEWLGLASLFIGLPAVISTVVSFSIPYYLLHNVTLANTLSTIIPIVVFAISVIYFNKYLQSRNLISTFMKKRSITILPDSGQPIDEKYIRGFEVNLKFIKGEEYIKQLAMLGMMYLQNAVAYDNKDLYLRAKEYLARAEEAMEGKSVSFETKMLVDYLRSKIETYKYRFGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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            <begin position="206"/>
            <end position="218"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18822"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="237" mass="27235" checksum="5C27ADA17F2A5852" modified="2011-06-28" version="1">MISAEILMKYIGQKVKDPYGRDFGYIVHVYTEVDGSVTGIEVAQGNSFNTIDPSRIKIDGDNIVVLPDWKAEAIKTLTYMEKIRKRQRALEELYAKQEIPKSTYDDMKRKLDTEMVKIRDDYTKIKNKLKQRLNDVEDQLTQIDKAMIAVKMSYIAAEMSESSYKNSIEILRQAKDSYTMEKDDIRKTLDKLDLLDKEGLDLKTPTPISNTSDQSNKNDQNKSDIPMPIPVKVINTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F2</accession>
    <name>F4B4F2_ACIHW</name>
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            <fullName evidence="2">Integral membrane protein (DUF2029)</fullName>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4F2"/>
    <dbReference type="STRING" id="933801.Ahos_0148"/>
    <dbReference type="KEGG" id="aho:Ahos_0148"/>
    <dbReference type="eggNOG" id="arCOG03701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491462_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="336"/>
            <end position="356"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="383"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="569" mass="65454" checksum="D18799ECC9C36C46" modified="2011-06-28" version="1">MTPLMDRNKALTVNFSQFLFAISMFLLIAPNYDNLLLITIDVLLIFFSGLMLFTSVHIHDKYFLTIIYTEAIVVGLFYYFAQGVSIYVLIIYLLLVAVGIKYNFLAEAMLVNFLILTLSTLAVFPNFGTDGMMLSYYAAYLFLHGENPYNPALTANIYSVFHLCKFIPIFGTPLSTGGVVTRYDYPALQMLILIPSVIFNINPNLITVAFYFLVAFLLYAKLRRVDKLLFLSFLLPYLFNINYRDFSAGGVTTLDWVFFVLLSLFFKDVKAKGVFYGLALSLKQTPFALLPFYLISIYKEEGKSSLLKFIIYTAISFLAINGYFIFLSPEYYFQGVLYPIVAPLIGISMSLSMIAISGFFYIYPLFFTLAIVIVMLTEVYFFWKYYDIFKDSWVFFPYFIFFVNYRALWNYFMSWPILGYALPNGERERINAINFKPLAIPVLLLISLGIFFHFAYTPYYTSINVKVLHICEKDGEIYGFLLNVSYYPKSPSLPNCIKPYFRIFPDAPSISANGYIFCSNATVLKKDSWEIVKLYPPYKGYYVPAVPLEVEAYYWNLIGIISVIPVNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G5</accession>
    <name>F4B4G5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774971.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G5"/>
    <dbReference type="STRING" id="933801.Ahos_0161"/>
    <dbReference type="GeneID" id="10599601"/>
    <dbReference type="KEGG" id="aho:Ahos_0161"/>
    <dbReference type="eggNOG" id="arCOG00177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Maltose/maltodextrin ABC transporter, ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047641">
        <property type="entry name" value="ABC_transpr_MalK/UgpC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875">
        <property type="entry name" value="MALTODEXTRIN IMPORT ATP-BINDING PROTEIN MSMX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43875:SF15">
        <property type="entry name" value="TREHALOSE IMPORT ATP-BINDING PROTEIN SUGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="309" mass="34812" checksum="41D403B5E882DB12" modified="2011-06-28" version="1">MSVELKDVSKKFGKLYALSKISLKVEKGEFFVILGPSGSGKTTLLRSIAGLEKIDEGKILIDGRDVTSFPPGKREISMVFQNFALYPNKTVYENLSMPIENLKKDEREKIIEEVSKRLGISQLLDRYPSQLSGGQQQRVGLARALVKRSKVFLMDEPLSNLDAPQRISARKLIKEIQLENSITTLYVTHDQTEAMALADRIAIIDQGKIIQVGSPEEIYENPVNEFVASFFGNPPMSVIHDGKGKLGIRAEDVKIGEGNYKGVVKDVEFWGDRYLIYISFNNEEIKAFSSKRLKIGEEINFDFKYKVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G6</accession>
    <name>F4B4G6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Extracellular solute-binding protein family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774972.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G6"/>
    <dbReference type="STRING" id="933801.Ahos_0162"/>
    <dbReference type="GeneID" id="10599602"/>
    <dbReference type="KEGG" id="aho:Ahos_0162"/>
    <dbReference type="eggNOG" id="arCOG00156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590033_0_0_2"/>
    <dbReference type="OrthoDB" id="18034at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901982">
        <property type="term" value="F:maltose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042956">
        <property type="term" value="P:maltodextrin transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015768">
        <property type="term" value="P:maltose transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061">
        <property type="entry name" value="MALTOSE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="468" mass="52211" checksum="F96C0D9AF821C311" modified="2011-06-28" version="1">MKGISKTIIALIIVVIVIIAVVGVYYITLHKSTTTPSISTTPPVTLTVVTFSGESAKFIQCAGNLFSEEHPGVTVKVICYPFSEYIDQEITALSAHSTEYDIIGFTSTSAQKVAPYLICLNESMFNMSDIIMPQEDFGGIIYNSTTGKTEMIGIAYETAVYLTAYKECIFCNSTLAEEFYNEYHMNFSPTTWENWTVVLDVDNFLTSHGITKYGFLIDDHVKHGIIDAYPAVFGWYYERCPELTQGKIGGLPGYNIMFESYILPGYSYPLPSFNSTAGIEALETYYDLVSYEPSPSSIQICYGNFAEFYPDAAGAFIFTSQLTCLNTTEREHTYLAPLPGDYAETGTDFLGVSKYSLHKQLAEEFLAFLVSPQVQKMAFLKFGKFPISKEAFQELMSNTSLPSYEIEWLTQVYRAAEEAWANPPNIPPTYPELIPSFNNEVYCYLTGKISAQEALNNAAQDWIKAVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H2</accession>
    <name>F4B4H2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoate-protein ligase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774978.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H2"/>
    <dbReference type="STRING" id="933801.Ahos_0169"/>
    <dbReference type="GeneID" id="10599609"/>
    <dbReference type="KEGG" id="aho:Ahos_0169"/>
    <dbReference type="eggNOG" id="arCOG03837">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181210_0_0_2"/>
    <dbReference type="OrthoDB" id="146287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10634" checksum="3820C7843B121227" modified="2011-06-28" version="1">MKIGIYELKAKKGVIRTTVKYYGDSLDIKITGDFMIFPEDIIFDLEKKLQGLSLNDLQNLPKILEETLSKATLFGCTIEDFKNSIYGALREVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J9</accession>
    <name>F4B4J9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinyl-diaminopimelate desuccinylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0196</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J9"/>
    <dbReference type="STRING" id="933801.Ahos_0196"/>
    <dbReference type="KEGG" id="aho:Ahos_0196"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010589">
        <property type="entry name" value="PRK13983.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="197"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="404" mass="45635" checksum="897F222183E1C401" modified="2011-06-28" version="1">MESLYKEIDNQRDFIISLYKEFIPIKALGPENGGEGEYSRAIKLKEIMEKYFDEVKEIDVKDERVPSKVRPNLIGTIKGRNQSKTLWIIAHMDTVPEGDRSLWKFDPFSITVDGDYIYGRGVEDDGQAIVLGITLAKVLKELNLTPNINFSLLISADEETGSKYGVSYLAKNYSLFNKDDIILIPDAGNPEGSMIEVAEKSILWLKFTVIGKQAHASTPEKGINAHLLSMILGVTLYEELHEKYDAKNELFEPPTSTFEITKVEKNVENVNTIPGKHTFYMDCRILPQYDVDDVLNTINEIVKRFEAENNCKVEVEVVNREDTTRPTDENSETVKLLKNAIKAVKGIDAKAIGIGGGTYAKYLRSLGLNPVVWMTCDETAHEPNEYVRISYILSDLKVISYMLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R6</accession>
    <name>F4B4R6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 16">Reverse gyrase</fullName>
            <ecNumber evidence="14">5.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rgy</name>
        <name evidence="21" type="ordered locus">Ahos_1413</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 or 2 zinc ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 14">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">Introduction of positive supercoils requires the cooperation of both domains. The helicase-like domain probably does not directly unwind DNA, but more likely acts by driving ATP-dependent conformational changes within the whole enzyme. A beta hairpin in the 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">This enzyme is the only unique feature of hyperthermophilic bacteria/archaea known and seems to be essential for adaptation to life at high temperatures. It may play a role in stabilization of DNA at high temperatures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the C-terminal section; belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 14">In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="14"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776211.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R6"/>
    <dbReference type="SMR" id="F4B4R6"/>
    <dbReference type="STRING" id="933801.Ahos_1413"/>
    <dbReference type="GeneID" id="10600903"/>
    <dbReference type="KEGG" id="aho:Ahos_1413"/>
    <dbReference type="eggNOG" id="arCOG01526">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002886_0_0_2"/>
    <dbReference type="OrthoDB" id="30963at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160097">
        <property type="term" value="F:reverse gyrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.510.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01125">
        <property type="entry name" value="Reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040569">
        <property type="entry name" value="Znf_Rg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01054">
        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505:SF1">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17915">
        <property type="entry name" value="zf_Rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52037">
        <property type="entry name" value="ZF_RG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52036">
        <property type="entry name" value="ZF_RG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 16" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="7 14 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RG N-terminal-type" evidence="19">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="97"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="17">
        <location>
            <begin position="576"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="20">
        <location>
            <begin position="754"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Topoisomerase I" evidence="14">
        <location>
            <begin position="572"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="14">
        <location>
            <position position="892"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS52036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AEE94296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1152" mass="130278" checksum="94177E7B4C63EE98" modified="2011-06-28" version="1">MVTSVFLKACPNCGGPLEDYRALAGLPCSKCLPGDISPFKNLSIDEKIRLIYNILVHENKLKGYWNLYYYHQLSQEIIDYFKKITGKEPWSLQKLWLRRLVEGSSFSMSAPTGMGKTTTIIVYSTYLQNSNKNVLYIVPTKSLMQQICEKMEKIGDKTSCGKVGSGISIVTVNYINRNFEEIKEYRPDFVAVDDADAIVKSGKTVDRLVTLMGIPQEVYEDAIKLVKLKRLLAIEEKRDEIETKIAEIESRISKYYGTASQLVVASATLRPKGIKQKALKYITGFDVSTAQIYARNIIDAFTSSSVVDIVNKLGSGGLILVSKEYGKDKIKEVNESLKELGFKTAIALSGRKFLEKFSSGEIDIIIGSASYYGVAVRGIDEPKRLKYVIFYGVPKSRVRLSQAIYNPYTLLKISKLFNVNIDDNLILSLSPSEAQLIKLAFLKSQKLTGKLGEIQKILEGKIDEVKSALKAVSNEVISDTFLIRKIGKEFYIEYPDAITYLQGSGRSSRLLNGGLTLGLAITIIDDFKLYEMLKRKLQYMIYNFNPTNFDSLDIGKVKNEIERSRNDKNEELHISTGLMIVESPTKARTIAKMFGAPARRNIAGVPIYETVIVDGNNVHILDIVATKGHVADLTTENIGYYGVEIRNEDKYPYYSPLYRCMNCKRVFSQEIDKCPYCGSTLISSSMSTINAIRKIAMEVEKVYIATDPDVEGEKIAFDVAANISAYNPNIYRVKYHEVTKKGILEALANPMNIDLNLVKSQIVRRIEDRWVGFELSKLLQIKFSNRNFGAGRVQTPVLGWITKRTEEYRKNMCYVASIKLGNYTHKVFLDNKNVKIDKVIVKKMEDKDDIILPLPPYTTDSLLIDAYNLYKLPAERVMKIAQELFESGLITYHRTDSIHVSNKGIEIAKEYLDKKGLIKSFIPRSWGNEGTHEAIRPTRPIDAEELKKEIEDNPQAYFIKFTWAHFAIYDMIFKRFLASQMKEAIGKYTKYQIKVLDNVSEITVLTSIKGGFSDVLKPRIYEIPEGEIQVNPIITKGSKVRLYTYAEVIKEMKDKNIGRPSTYAKIIQTLMRHGYIVESKKKYALIATKKGINVYNYLNSKFSSLVSESTTAELLEKMDMIAGGKLNADNVLTEILGQIKSLVTSLNSEQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R8</accession>
    <name>F4B4R8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1415</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776213.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R8"/>
    <dbReference type="STRING" id="933801.Ahos_1415"/>
    <dbReference type="GeneID" id="10600906"/>
    <dbReference type="KEGG" id="aho:Ahos_1415"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="OrthoDB" id="372104at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="28"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="234"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="917" mass="105896" checksum="D4F0E950D4E33EA7" modified="2011-06-28" version="1">MRKLDDRLLELMKERNWKNMTEIQQKSLEPILSGKNTLIIAPTGYGKTEAALIPILDMMLKTNVKPVSLIYITPLKALINDLTLRIEWWASRLGFYVNRKHGEVPQKEKNLRLKITPHILVTTPEGLEIDLDWATRFREKYKNVKWVIVDEIHELIGSKRGVQLSVLLERLKDYIGYDFQRIGLSATINDEDLVASFLFGSSSRQSEIVKVNDKKEFELSIVKLNSAGDIWKNVAKHIISSVEKPSLIFTNSRFLTERLHEELEEYDKDFYVHHSSISRESKSNVEEYLRTGKAKGVICTKTLELGIDIGDIKKIIMFRPPPSVASFLQRLGRSGHKIYGKPRGEIICIYDHDLLEAYAISELAKLGKVERQSICKPLDVAAREILGMILQYDEVEKEKAYKILTSSYVFRNLSEKTFNELIEYLAKNNLITIKGDKLSIGKFFFKLWNFNRNNGYSWSRGFSEFFSMINSTDTFTLKWEDKNIGEIDAIYVYKHIRSGDIIRIGGKLWKIIRINTSRMVVDVLPADKGDGEIPVWRGDGISKSYLLPKEIYVLFKEKRKEISDIIDKYVSRNLPLPSPNVIIEEKRDKEIVYSTLINEKIASTLAHILLYLATSKDSLNNYARYSIYGFSINYTEKDLLEELLKYNEKELKRIIIRSILRSPLFISTLKEIQISYGKLGKIDTKEDKLIIKDALRQTISKYFSIKNTIRFINKIKRGKIKIIKYDGSTPLGDAVLLQAPIKPWITGISTIIYDTLKGGAYTLAELSESLGISQKSLEAKLKQMRKPESKYRIVSFIDVDSRETRWCLMEDLENIVKSCDFYTSFNPINSDETFIASLRAINSTTTTDLIFKPKEIIDNPEEIIRRIPFEEIGEMRINDPVDPLVYNISPRFYYVNKRVAPYLLLNAVAYIQNMKYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S1</accession>
    <name>F4B4S1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Probable deoxyhypusine synthase</fullName>
            <shortName evidence="11">DHS</shortName>
            <ecNumber evidence="6 11">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dys</name>
        <name evidence="12" type="ordered locus">Ahos_1418</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 11">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6 11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776216.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4S1"/>
    <dbReference type="STRING" id="933801.Ahos_1418"/>
    <dbReference type="GeneID" id="10600909"/>
    <dbReference type="KEGG" id="aho:Ahos_1418"/>
    <dbReference type="eggNOG" id="arCOG04142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_1_0_2"/>
    <dbReference type="OrthoDB" id="17730at2157"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000007">
        <property type="entry name" value="Probable deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002294">
        <property type="entry name" value="PRK01221.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="311" mass="35102" checksum="29F5AC8B12E916CF" modified="2011-06-28" version="1">MKREDLLTEVVGDITLEDIKGLDKYIELFNKIYGFSAEALYRGAKILKDMQKDADLRFISFTANLVSTGLRGLFADLIRNGYFNVVITTGGTIDHDIARSCGGKYYKGSFDYDDNMLRELHIHRLGNILVPMESYGLVVERVVKNVVDEIVKEKKEWAGYELLWEFGKRINDPHSILKAAYEKKVPIIVPGIVDGSFGTNLFVRSQFTGLRLNLFEDMRMVKDLVFSCKKSGALIIGGGISKHHTIWWNQFKDGLDYTVYLTTAQEYDGSLSGAKPREAISWNKIREHSENVVIYGDATIILPILASALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S2</accession>
    <name>F4B4S2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type 2 DNA topoisomerase 6 subunit B</fullName>
            <ecNumber evidence="7">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DNA topoisomerase VI subunit B</fullName>
            <shortName evidence="7">TopoVI-B</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">top6B</name>
        <name evidence="9" type="ordered locus">Ahos_1419</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TOP6B family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776217.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4S2"/>
    <dbReference type="STRING" id="933801.Ahos_1419"/>
    <dbReference type="GeneID" id="10600910"/>
    <dbReference type="KEGG" id="aho:Ahos_1419"/>
    <dbReference type="eggNOG" id="arCOG01165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006403_0_0_2"/>
    <dbReference type="OrthoDB" id="65493at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000091">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000062">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00322">
        <property type="entry name" value="Top6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005734">
        <property type="entry name" value="TopoVI_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003218">
        <property type="entry name" value="PRK04184.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01052">
        <property type="entry name" value="top6b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006553">
        <property type="entry name" value="TopoVI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="95"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="527" mass="60822" checksum="771CF3CC3EACBB48" modified="2011-06-28" version="1">MSEKFASISPAEFFKRNPELAGFSNPARALYQALREMVENSLDATDVHEILPSIKIIMDLIDQDKQIYKLTVEDNGIGIPPHVVPDAFGRVLYSSKYVIRQTRGMYGLGVKAAVLYSQMYQDRPVEIMTSPINSKRIYFFKLKIDVTKNEPIIYERSSILNDKGFHGTSVTLYLLGDWQRSKARIYEYIKRTYIVAPYAEFFFKDPEGNVVYYKRLTTKIPEPPKEVKPHPYGVDIELLKNMIAKQKNDYEVKDFLTKEFQSVGDVTALKILEMAKINPDKKIKSLKDDELARLTEAMKKFEDFRPPSPDALSVIGADLIELSLKNMFNPEFVAALTRKPKAYQGHPFIVEVGIAYGGAIQPMDEPLVLRYANKIPLIYDEKSDVIWKVVEEIDWKRYGIEEEQKPLVVMVHLCSTKVPYKSAGKESIADVEEIEKEIKNGIMEVSRKLRLYISEKKKEEEARKKMLTYLKYIPEIARSLAVFATDDKKELIPKVQDEIQSKLFEIVKKKLDVKDEEEYKMYKVEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U1</accession>
    <name>F4B4U1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775023.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4U1"/>
    <dbReference type="STRING" id="933801.Ahos_0214"/>
    <dbReference type="GeneID" id="10599654"/>
    <dbReference type="KEGG" id="aho:Ahos_0214"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726883_0_0_2"/>
    <dbReference type="OrthoDB" id="34376at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06174">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
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        </location>
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        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
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        <location>
            <begin position="163"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="378" mass="42102" checksum="F9F3385485BD4559" modified="2011-06-28" version="1">MNRRTLIYAVIIVIITFSLRASNNMIITTLPLIAKYYFHFSSILIGVISSLAMLSAFIASGLINSRLTSSIRRKVFITSAIIYSTVFPLFYFSNPYNVWLLTSLVGFSLGMIMPNIITSAGLFQDQRTRERMLSLYTLALSTSLIIGPLIESAILLKFTLFQAFIFFSILPALVAISSFLVKFPEEEENERVKVKVWKNPGFRISIFLNLMYALPFGTLTTFGGIYAVDSFHASYSLATALFGMFFATSFLGRLMMTILPPRDLGLPIWISASLTIIGLSAIFLSNSLILYIIALIILGIPHGLTYPTSLIALTRSFPEEERNVANSYFSATMTAFTSFVPIIISTIVNSIGIRYSFALLIPVSLAFFIGVLWNERSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W4</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W4"/>
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    <dbReference type="KEGG" id="aho:Ahos_0237"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324081_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="233" mass="26971" checksum="E5F3FA2B786EA827" modified="2011-06-28" version="1">MSSFNTKFFNFENKNNTVIELSKYACSEIFNEKEVEILCKIIKEPKSLYKLYIDTNIPIATLWRIVNRLRENGYLVHEKRGVYKVTYKGALLIYLVGCNKFKELSLKYISELYNLCINKVKNILNYIIEVSNKYSICVLEFDDIIKIIPFVLFDALNGLYIPSDTIEFVLKALNGHPSVISDEGCHIIPEITNEGEKILLGKCKNNGFVSNHKCPHVRKILDKKFSKEFLILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X5</accession>
    <name>F4B4X5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="ordered locus">Ahos_0248</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0248"/>
    <dbReference type="KEGG" id="aho:Ahos_0248"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
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        <location>
            <begin position="58"/>
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        </location>
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        <location>
            <begin position="108"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="155" mass="17518" checksum="780383517A55B66B" modified="2011-06-28" version="1">MAASIFILSLLILLDIYGINYAFSRIAQIIMWIIWILSFPSYLSYNSSLLEKRKILEYLAPIAIIITLIGLIFLAFGKFIGIELIVFGYIFEPIAGISIFLTLKKVSVLFSSLFFYGAIVFTAGLPLFLFNLGIISIIGDIIKMIGLSYFIYKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B505"/>
    <dbReference type="STRING" id="933801.Ahos_1424"/>
    <dbReference type="GeneID" id="10600916"/>
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    <dbReference type="eggNOG" id="arCOG01015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747264_0_0_2"/>
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    </dbReference>
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        <property type="entry name" value="Pus10_THUMP_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011139">
        <property type="entry name" value="PRK14554.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22023">
        <property type="entry name" value="Pus10_THUMP_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pus10 THUMP" evidence="1">
        <location>
            <begin position="114"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="357" mass="41868" checksum="24DCC82F3F37E8E4" modified="2011-06-28" version="1">MSKNSLDNLEEKALELLKKYPLCDSCLGRCFAKLGYRFANKERGKAIKTYLVLELDRKIKDHELEDLNEIKEILFNMGKEYSGIFEIYFSNEKFQERTCYICNSEIEEIKEDFFKKALDFLKKRGKVKYVLGVKLSEQLSRLENEFIFSNGLIYYESIRNEIKREVGKMLAKEGYEPCIENPDIDIIYDLGTRSIYTISKKYKTLYLYSRLSRRVPISSWYGKDSLEEEFNGKEILVPFTEPSEVRILDEYPLIISDEDREEIEANGYFMRKIGKIAGREISAIMTAKPEKRIYSVLIYSKEEIGERVLGNIRLLTVEARDFKELKEKLKDIKGEIISIDLIESEGKHKILENKLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B515</accession>
    <name>F4B515_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <gene>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776232.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B515"/>
    <dbReference type="GeneID" id="67200062"/>
    <dbReference type="KEGG" id="aho:Ahos_1434"/>
    <dbReference type="HOGENOM" id="CLU_1567045_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="170" mass="19324" checksum="FC3C4AC6173F9619" modified="2011-06-28" version="1">MWKKKFSLLFHVQVVEVTKEDIIQAVKEDEDLSPYDALHYVISRRYSNSQCGSRLQRKDRPLFFLFIKIKTRAKSKIYRIINISNSSLLKNYRPRKTPYYVNIHRKNEKLALNLVTVQEYTCTNPPGKNTPEGACDYNGEVSTPEPITLGSLLLTGEAESGVSSPAPNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M2</accession>
    <name>F4B5M2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M2"/>
    <dbReference type="STRING" id="933801.Ahos_1563"/>
    <dbReference type="GeneID" id="10601064"/>
    <dbReference type="KEGG" id="aho:Ahos_1563"/>
    <dbReference type="eggNOG" id="arCOG10175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086888_0_0_2"/>
    <dbReference type="OrthoDB" id="26975at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="270" mass="31496" checksum="F5D06FF99744059F" modified="2011-06-28" version="1">MRTEVLVYTVVISAFILSLLFYPVFEVNSSIYSQELGLFGFNIRFYDYSSTVFLPLPIAGVIYALLSAIVPLKWRKTRYSLYLSTILILLSISMFISSYIYDEKYLFYYDYSVLPTYNGAFLIYFPKVFSFNLPFFILIASAVISSLNSVTRARWIPISKMSLLEKVLHDLRYNEIERGIVKLFTELNVPYSIEEGVISAQHLKILKENRKIKEAFFPRRFNTSVIFIQGNKAYKMDNGELKEISLLEAIKIILEKVEINENKIEKEEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M5</accession>
    <name>F4B5M5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Photosystem I assembly BtpA</fullName>
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    <gene>
        <name evidence="2" type="ordered locus">Ahos_1566</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BtpA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776364.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M5"/>
    <dbReference type="STRING" id="933801.Ahos_1566"/>
    <dbReference type="GeneID" id="10601067"/>
    <dbReference type="KEGG" id="aho:Ahos_1566"/>
    <dbReference type="eggNOG" id="arCOG01982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075239_1_0_2"/>
    <dbReference type="OrthoDB" id="38543at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04722">
        <property type="entry name" value="TIM_phosphate_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005137">
        <property type="entry name" value="BtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00259">
        <property type="entry name" value="thylakoid_BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SGC REGION PROTEIN SGCQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381">
        <property type="entry name" value="ZGC:162297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03437">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005956">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="262" mass="28622" checksum="3B9FDB2AE2AE35BE" modified="2011-06-28" version="1">MLEKRKPFIIGMIHLPPLPGSPNNKMSLDEIVNYAITEGQKLQEAGVDGVIVENLGDYPFFKDNIPPITIASMSIIVREVRKNFYFDAVGVNVLRNGCIDAFSIAHVTGSQFIRCNVLIGAYVTDQGIIEGKAAELLRLKRFLNSNVMIFADIHVKHAYPLYNLPIELAAQDLAERGGADAVIVSGPRSSIPPSVDTVKKVKSSVSLPVIIGSGISLENFKEFCKIADGLIIGEKDFKEGGTIGGPSKKEAYEYLVKECRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S2</accession>
    <name>F4B5S2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S2"/>
    <dbReference type="STRING" id="933801.Ahos_0399"/>
    <dbReference type="KEGG" id="aho:Ahos_0399"/>
    <dbReference type="eggNOG" id="arCOG00818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF7">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, SPOVT_ABRB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
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    </dbReference>
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        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="11512" checksum="305589BC19ABEE41" modified="2011-06-28" version="1">MFNLRISKVKLKAFMRILFYMYTLKVHKKGIIVIPKEIREKFNLKEGDEVNVIVDDEGIYIFPRDDIRKYFGSDKDAIKALNILEEERRRERESST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S3</accession>
    <name>F4B5S3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775204.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S3"/>
    <dbReference type="STRING" id="933801.Ahos_0400"/>
    <dbReference type="GeneID" id="10599840"/>
    <dbReference type="KEGG" id="aho:Ahos_0400"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135601_2_0_2"/>
    <dbReference type="OrthoDB" id="147997at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18689">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14389" checksum="49B1449B1C82C896" modified="2011-06-28" version="1">MKVVLDASTLILYFYGNNKAKEIIEKNMNEAFINSVNLTEFLYSYAKVKGWKESLFKYTLIRDTFKLIDVNSEDIIKSVAKLKIKYNLSLGDAFLLASAKSTNSTAVTSDHELSKVNEVKVLLIPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5T6</accession>
    <name>F4B5T6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775217.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5T6"/>
    <dbReference type="STRING" id="933801.Ahos_0413"/>
    <dbReference type="GeneID" id="10599853"/>
    <dbReference type="KEGG" id="aho:Ahos_0413"/>
    <dbReference type="eggNOG" id="arCOG00713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125353_2_0_2"/>
    <dbReference type="OrthoDB" id="40200at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052106">
        <property type="entry name" value="PINc/VapC_TA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826:SF5">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="15198" checksum="FDD8BB4B100CFE63" modified="2011-06-28" version="1">MIFLDANFIIYLNLGVKEVVNFYIKVLQEDRLALDPLVIDEVIYISKKKYNVNFNDTISFLDEVVLPNSLILPIRKEDYDKAKEIMLQYNLKPSDAFHVAIMLNNSISKIISEDKNFDRIKEIERLWLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5U6</accession>
    <name>F4B5U6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5U6"/>
    <dbReference type="STRING" id="933801.Ahos_0423"/>
    <dbReference type="KEGG" id="aho:Ahos_0423"/>
    <dbReference type="HOGENOM" id="CLU_2519557_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9767" checksum="55224D2C90C7FDCA" modified="2011-06-28" version="1">MGVFPGIYLPLPFFAYYLSISLISYTLWGFYFYSSISIILSSQYKILSTRSSHNLLQIMYLSKDSITILILNFIIAFNNLQTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V3</accession>
    <name>F4B5V3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775234.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5V3"/>
    <dbReference type="SMR" id="F4B5V3"/>
    <dbReference type="STRING" id="933801.Ahos_0430"/>
    <dbReference type="GeneID" id="10599870"/>
    <dbReference type="KEGG" id="aho:Ahos_0430"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="165" mass="19214" checksum="157A77B9080F0547" modified="2011-06-28" version="1">MELDEVDLKILKIIQNDAKYPLEKIAEEVKVPKSTVAYRIKKMEKMGVIKGYFTYVDPASLNLDYLVITLVRARYGKDYHDSLGKKLSQLPGVWGVYFVLGDMDFVVLARFKNREDFMKNFLEKLINMPEIERTSTHVVAKVYRETPYVTIDEEKVNIEEIKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V4</accession>
    <name>F4B5V4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aminotransferase class-III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0431</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775235.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5V4"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="441" mass="49777" checksum="FD25AE1FD5F11175" modified="2011-06-28" version="1">MDEDVVKLVKEHTFGTWKVQKTWNPLNVDKAEGVYFYANGKKILDFSSQLVNVNLGYGNKEVIKSIEDQLENLQYISPAFATEVRARAVKSLLEVMPSNLTKFFFSTSGTEANEAAIKIARFYKSPSYKILSRYRSYHGSTLGSLALTGDYRRWYAEPNVAPGVVKIPEPYCYRCPFKLKYPECNLACVNYVDYVIKNEGNVAGVIVEPITGTNGVIVPPKEYLPTLRKITRENDVLLITDEVMTGWGRVGEWFAVNLWGVQPDILTTAKGASASYVPIGITAVSKEIADFFEDKMFAHGHTFEAHPVSLSAIPAVIEEYKRMDLLAHVKAMGSYLGERLKELKERHKSIGDVRGVGLFWAIEFVKDEKQTPFGTYEDKYEGRVTEVDILAKKLLDEGVYVFNGPSWLVISPPLIIKKEEIDEGIEKLDEKIKYLDEKFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B618</accession>
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        <submittedName>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B618"/>
    <dbReference type="GeneID" id="10601138"/>
    <dbReference type="KEGG" id="aho:Ahos_1638"/>
    <dbReference type="eggNOG" id="arCOG12789">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1323952_0_0_2"/>
    <dbReference type="OrthoDB" id="39885at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="24661" checksum="BED603A1FFE989C7" modified="2011-06-28" version="1">MLEREVKGSIEEDEMKILSDVYDINNLREWWMFLRKIEKIDEENYRAEFKVFMTFKFHMKRTLGSHEVIHEGTMSFPRAYFRFIVETIPYKKDKKVDVIIRGQYKGPLERLATLPMDIFLKNFFQRLTERYKTKTEEGEKQNILSLINEQLEASREYNGRIILHLDECTIVFKGGKVEEVSCNGLKGDDALKELTKKENAKIKVEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B620</accession>
    <name>F4B620_ACIHW</name>
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        <submittedName>
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    </protein>
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    <organism evidence="1 2">
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        <lineage>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776433.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B620"/>
    <dbReference type="GeneID" id="10601140"/>
    <dbReference type="KEGG" id="aho:Ahos_1640"/>
    <dbReference type="eggNOG" id="arCOG08348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153633_0_0_2"/>
    <dbReference type="OrthoDB" id="42168at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="15528" checksum="7667BCE4D3DCD787" modified="2011-06-28" version="1">MKEVIVRTRLSCNYRRLKAFFISSGLVKLFYYVDKIEKRGPYQYVINDKYDAELFILDRRITWEVYKDRDLKDRIEVELIPLTDNDTAVFLKFSTNRILPIKKVLEKEVNSEVDLLRELLDSLRTFNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B646</accession>
    <name>F4B646_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0446</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="RefSeq" id="WP_013775250.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B646"/>
    <dbReference type="STRING" id="933801.Ahos_0446"/>
    <dbReference type="GeneID" id="10599886"/>
    <dbReference type="KEGG" id="aho:Ahos_0446"/>
    <dbReference type="eggNOG" id="arCOG03721">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="OrthoDB" id="42749at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14587" checksum="66F774349E6C47B6" modified="2011-06-28" version="1">MIRVLTVPDVLVLNADELLSRGDVVQASEKYYKAVEEGIKLLARKYELNDILSRVKEKGFWDVKDLDDAVYELSKMLNKKEIIEYWESAILIINTSLYPEDLREEAKKVKEFVSLFDEITNRDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B673</accession>
    <name>F4B673_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-CoA thioesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0473</name>
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    <organism evidence="1 2">
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B673"/>
    <dbReference type="STRING" id="933801.Ahos_0473"/>
    <dbReference type="KEGG" id="aho:Ahos_0473"/>
    <dbReference type="HOGENOM" id="CLU_3412274_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="28" mass="3257" checksum="A139BA192CB21565" modified="2011-06-28" version="1">MAQAEFVFEETVRIYDTDTQGIAHYASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B680</accession>
    <name>F4B680_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cobalamin biosynthesis CbiG protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775284.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B680"/>
    <dbReference type="STRING" id="933801.Ahos_0480"/>
    <dbReference type="GeneID" id="10599920"/>
    <dbReference type="KEGG" id="aho:Ahos_0480"/>
    <dbReference type="eggNOG" id="arCOG00652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010546_0_0_2"/>
    <dbReference type="OrthoDB" id="4722at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="CobE/GbiG C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="288" mass="32666" checksum="F05F3EB7B29991A5" modified="2011-06-28" version="1">MYVSRIKIIAESSNELAKKVRNSLDELGYFIVDNKEEVQVYFYPIEEIIYRVKKFSTKTPVIIGVTDDGSYVIPLFKENCGGSFIAGMIADLLGSQLILTSRTSQQGVYSIQEFAWINGLEIINREKIDELEKKLLNSGKLKVYSNIDIHTVEGYELTRKEEDADIIIKTDTEEEDKEIDDKKIIMKPLSLVIALSYSKDTPKEAIYYSIISTLKSINILRSTVNFIITSESKNGDKKIQDIAKSFNSTVIYVKEKQPCEPSLDFVNARVILKKTKRAYGVLTCLGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D2</accession>
    <name>F4B6D2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776467.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D2"/>
    <dbReference type="GeneID" id="10601175"/>
    <dbReference type="KEGG" id="aho:Ahos_1675"/>
    <dbReference type="eggNOG" id="arCOG06096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271157_0_0_2"/>
    <dbReference type="OrthoDB" id="42638at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="102" mass="10759" checksum="67086809DF3CD95D" modified="2011-06-28" version="1">MVVEKVLATIGGILTLMAGIVDIATKNPLAHYIYGLNIISGSIMIYLGILGIVAGLMTLYGEYKNNRDFVIAGGALGLATPTEFSLLSLIGGLMMQKEKVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H4</accession>
    <name>F4B6H4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Formate dehydrogenase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ahos_1717</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776509.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H4"/>
    <dbReference type="STRING" id="933801.Ahos_1717"/>
    <dbReference type="GeneID" id="10601217"/>
    <dbReference type="KEGG" id="aho:Ahos_1717"/>
    <dbReference type="eggNOG" id="arCOG01492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_2_2_2"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.90:FF:000001">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000003">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.228.10:FF:000002">
        <property type="entry name" value="Formate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000032">
        <property type="entry name" value="Formate dehydrogenase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000005">
        <property type="entry name" value="Periplasmic nitrate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF14">
        <property type="entry name" value="FORMATE DEHYDROGENASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036643">
        <property type="entry name" value="FDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="16">
        <location>
            <begin position="77"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="15">
        <location>
            <begin position="254"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE94594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="977" mass="109258" checksum="2BC7C2110840DB6D" modified="2011-06-28" version="1">MTVKISIDNRETTANEWETILSVLKRNGVYLPHICYNEGLTPIESCDSCLVEVNGRLVRACSTKVSDGMNVLVNSPRAVNARKTAISRILKYHKLYCSICENNNGDCVLHEAVIKLGINSQKYVEKPYSVDDTGPFYIYDPSQCILCGRCVEACQDFAVNEVIWINWDLNPPRVIWDNGNPIGNSSCVNCGTCVTVCPVNALMEKSMLGEAGYLTWINKDLKKKAIEAIGRAEDSFSLLMTFSEIEAKARESQIKKTKTVCVYCGVGCSFEIWTKGRKILKVEPKPESPANGILTCVKGKFGWDFVNSPDRITKPLIREGDRFREASWDEAIEYIARKLKEVKEKYGPDSIGFIASDKMSNEEAYLLQKLARAVIGTNNVDNSARYCQSPATVGLWRTVGIGADSGTIKDIAEYSDLIIIVGHNTTESHPVVGSKIKRAQKIRGAKVVVIDVRKHEMAERANLFIRPKPGTDAAILAAVAKYIIDKGWENREFIEKRVKGFEEFKESIKGFTLDYVESISGVPKEQIIKLAEMIHEAKNVVILWGMGVTQHLGGADTSTIISDLLLITGNYGRPGTGAYPMRGHNNVQGVSDFGCLPNYLPGYQKLDDENVIRKFEDSWGVKLNRKPGLQITQMIEGVLEGKIHALYIVGEDTVMVDCGTPLTKQALEKVDFLIVQDMFMTETAKLADVILPAAASLEKDGTFVNTERRIQRFYKAMEALGDSKPDWEIIQMIANALGANWNYKHPSEIMDEIAKLCPIFAGVSYSRLEGFNSLLWPVNEDGTDTPLLYVNAFATEDGKAILYPLSWKPPELRDEVHRVIVNTGRVLEHFHVGNMTRRVEELRRKVPETFVEVSKELAVKYSIKNGDLVLVKSKFGGEIKARAIVSDRVKGEEIFIPLFASDPSKGVNNLTGLEIDKASGTPGYKDTPVVIEKIEEGKGESPLPLDNWRFHINERRRQLGIEVQKKWMREEFRPLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K1</accession>
    <name>F4B6K1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GINS subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775327.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K1"/>
    <dbReference type="STRING" id="933801.Ahos_0523"/>
    <dbReference type="GeneID" id="10599964"/>
    <dbReference type="KEGG" id="aho:Ahos_0523"/>
    <dbReference type="eggNOG" id="arCOG00552">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117967_0_0_2"/>
    <dbReference type="OrthoDB" id="36229at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="1">
        <location>
            <begin position="65"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="179" mass="20321" checksum="4DD38253B1FCA231" modified="2011-06-28" version="1">MFKVLLRALDKLQLTEKKKVAVFDDIGPLDLGFTEISLSKGSEDEIPLWLAQHLEKQNLVKINSITTEELGKLLFQEKQNSTTPASLVKTYPDIYFRIKKLEEELKAKGLDGLEELKKVKSMVNEISLIRLRKIIQLASLNINDQTLISKMTKEEYLAYLSMRKIITSFYGGVIGSSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W1</accession>
    <name>F4B6W1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6W1"/>
    <dbReference type="STRING" id="933801.Ahos_1778"/>
    <dbReference type="KEGG" id="aho:Ahos_1778"/>
    <dbReference type="HOGENOM" id="CLU_161776_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="10706" checksum="BD671E35FAC7565A" modified="2011-06-28" version="1">MKKWILSFLVLLLLMPVIPTSAHYEIIGGGGGPPYSPYSFSKTISTSGLETKPGCTLGTANWWSGVSLCGTNLGILCNNKHGGNNVSTSSVYFNVTFYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B708</accession>
    <name>F4B708_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methionine adenosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B708"/>
    <dbReference type="STRING" id="933801.Ahos_0603"/>
    <dbReference type="KEGG" id="aho:Ahos_0603"/>
    <dbReference type="eggNOG" id="arCOG01678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="entry name" value="S-adenosylmethionine synthetase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003365">
        <property type="entry name" value="PRK04439.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003366">
        <property type="entry name" value="PRK04439.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="403" mass="44527" checksum="68D1F1ADBB15DB4D" modified="2011-06-28" version="1">MKNIVVEPARVQDINNLPVELVERKGTGHPDYIADSASEEASRKLSLYYLKKFGTILHHNLDKTLVVGGQAQPEFKGGEVIQPIYIIVAGRATTEVRTEDGIQNVPVGTIIVESVKNWIKNNFRYLDPEKHVIVDYKVGKGSTDLVGIFNKGKTVPLSNDTSFGVGFAPFSTLEELVYQTERMLNSKEVKSELPEIGEDIKVMGLRRGKEIDLTVAMSTISELIDDTNHYLAIKEEAKQKILDLASRLAPDYKVNVYINTGDRVDQGILYLTVTGTSAEHGDDGMTGRGNRGVGLITPMRPMSLEATAGKNPVNHVGKIYNVMAMLISKKIMEETKIRNVQVEVLGQIGRPINDPLVVNVEVGTENGKISDDLKNEINGIVDEYLESFNKITELILDGKVSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B735</accession>
    <name>F4B735_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="2">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0630</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B735"/>
    <dbReference type="STRING" id="933801.Ahos_0630"/>
    <dbReference type="KEGG" id="aho:Ahos_0630"/>
    <dbReference type="eggNOG" id="arCOG04228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="12732" checksum="158B4CBA6B100184" modified="2011-06-28" version="1">MVVMVRNDIKMGKGKIAAQVAHAAVSLVLDILNSNNNIWKSCLEEWINEGQPKIVVKVENLEELLKRAELARQKNLPVTIIQDAGKTQVEPGTITCAGIGPCEDSLIDSITGDLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B740</accession>
    <name>F4B740_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0636</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B740"/>
    <dbReference type="STRING" id="933801.Ahos_0636"/>
    <dbReference type="KEGG" id="aho:Ahos_0636"/>
    <dbReference type="eggNOG" id="arCOG05937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502157_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="6">
        <location>
            <begin position="189"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="526" mass="57878" checksum="A86E3616A6E9CE76" modified="2011-06-28" version="1">MTGDEALAYVLKEIGTKKVFTTVSLPENLRERLKEYEIEQDISLNSRDALLLAETYAMENNTAGVVIQIPGTEILNGIDVIAQAYADSVPLLIIGSLRSYRDVGRARIGELRTPDDLSSTLAPITKLRERAISIEEITVTVEKANKEALSNRPRPAFVEIAEDLFKMKAYPLSPAEQKPEKRTPDKNTVAKVAEVLSNSKLPVIVSGYGVITSGAYNELMELAELLDAPVITTIKGKGSFPASHQLFAGEGLGIIGTDIGNKLLAEADSILFLGTRLTQLSTGGWSMKFKGFTMHNSIDGEDIGKVIMPHLPIVADTGLFLKELLVLLKQKIKDKIDRGIRYEILRSKKEPELRAHSGLWPWDVVKTLMDFKFSKIFVDLSAVTFDMIRYPVEKPVWFTSESIISKGIGIAGVIQSKDKNALGVTDLPSITRNLGLLQSKKDEAKGALIIFNDKASTYLDTTASDAPTIKRSWSSVNIDLKFLGAIDVNSTAELKEALNNNEGDNKLRIINVNIDPQYDSSVLIRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B754</accession>
    <name>F4B754_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyl transferase family 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B754"/>
    <dbReference type="STRING" id="933801.Ahos_0650"/>
    <dbReference type="KEGG" id="aho:Ahos_0650"/>
    <dbReference type="eggNOG" id="arCOG03199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_4_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06856">
        <property type="entry name" value="GT_GPT_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926:SF3">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE ALPHA-N-ACETYLGLUCOSAMINYL 1-PHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="318" mass="34645" checksum="6A90F7A7F1FEDD6F" modified="2011-06-28" version="1">MLPICALIAFIITFLTTRWVIHIAKERKIVGKDVNKPNKPEVPSLGGIAIVAGFVSGTFVLLIQDPNYERIISSILLSSLLIAFLGLMDDFLNLRQSIRAFTPIFAAVPLAVFSVGHSAISIPFLGVVNLGIFYYVIVIPAALTISSNAFNMLEGLNGLGTGMGIIMSASLAYIGLTRSGNIFVAGEMALILLASLLAFLYYNKYPAKVFIGNIGTYFVGSVIGSIGIAGYMYTALAVLYLPYVAEFILKARTRFKGVSFGKVNEDGTLYWNDMPNSLTHVVMKMGRFKEYQVVAILWIIEGIFAILAIYLQTTVIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B789</accession>
    <name>F4B789_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776624.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B789"/>
    <dbReference type="GeneID" id="10601334"/>
    <dbReference type="KEGG" id="aho:Ahos_1834"/>
    <dbReference type="eggNOG" id="arCOG03852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665579_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16967" checksum="A92C1B9B63AFC407" modified="2011-06-28" version="1">MESRIKEYLQSEDKELKHEAWNYVEAHYKELGKDFIIEMLKFPDTGTRYRAWNFVKKLVEEKFLTVEECKALSPYFLEMLKDNNVVVRALSWYVTLIPLIDIGIIDKEKVVKEYSRYLCEIKEQEYKDVLEEVREELGIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A3</accession>
    <name>F4B7A3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A3"/>
    <dbReference type="KEGG" id="aho:Ahos_1850"/>
    <dbReference type="HOGENOM" id="CLU_2839167_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="65" mass="7135" checksum="E48EAA636C6F5259" modified="2011-06-28" version="1">MPSSLLIHKDMPMAKALKNIIEDAKPIARGLFASESDGFGDCPLKKKIANMTANITEVDFIIFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G5</accession>
    <name>F4B7G5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo12</name>
        <name evidence="8" type="synonym">rpoP</name>
        <name evidence="9" type="ordered locus">Ahos_0683</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775486.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G5"/>
    <dbReference type="STRING" id="933801.Ahos_0683"/>
    <dbReference type="GeneID" id="10600135"/>
    <dbReference type="KEGG" id="aho:Ahos_0683"/>
    <dbReference type="eggNOG" id="arCOG04341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179456_2_0_2"/>
    <dbReference type="OrthoDB" id="129238at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001604">
        <property type="entry name" value="PRK00398.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
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    <keyword evidence="1 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
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    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
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    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        <location>
            <position position="29"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J9</accession>
    <name>F4B7J9_ACIHW</name>
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        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS15</fullName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
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        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS15 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17444" checksum="A70960453C0FEEC0" modified="2011-06-28" version="1">MNKKRARGKSHSTRPVRAGAPKWVRFTREEVEMLVEELAKRGYGPSMIGVILRDQYGVPLVKQITGKKLTLILEEKGLAPKIPEDLFNLIRKAVNVRRHLTEYPKDKVSKKGLEEIESKIRRLVDYYKEIGKLPANWNYDPTTAELLITSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M2</accession>
    <name>F4B7M2_ACIHW</name>
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        <submittedName>
            <fullName evidence="9">Transmembrane protein</fullName>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M2"/>
    <dbReference type="STRING" id="933801.Ahos_1896"/>
    <dbReference type="GeneID" id="10601395"/>
    <dbReference type="KEGG" id="aho:Ahos_1896"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="OrthoDB" id="44119at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="14"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="421" mass="45623" checksum="7C53CEC8B4D00FFA" modified="2011-06-28" version="1">MKDIKIPSKFIRVVVILSTIGLIIEAYDFFSIGIISTAIWPYVFFKNASSSAIAFSILAYATILIGRPAGGVIFGHFGDKIGRKFSMFWSLFLSGIAMLLVALMPPIGVLAVLFIAILRFMQGMGLGGDGGSSLVLSYEYAEKGERPGYLMSFPMAAAILGIILGVLGVLISENLESKLFLLTYGWRILIGIGAIAIMVSAYLRMKVVESLDFKRLLNNNEVDPSPIKNAFKRCGKNIVLITLAIAYFPVILNFLLYPYSIELFTKMGFAEKQVMFTFLIASVLAYGFSILGGYLADKYGWKKIVLISAVGSLITLPILFTHLLMSLFPLYILASMGWGAMGTYASEFIISLRNTSAGAVFGFIGLFPAVLLIAVLPALITVYGVVGSLMPISIIGELITVVSIISILILIKARKVEGSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N7</accession>
    <name>F4B7N7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776699.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7N7"/>
    <dbReference type="GeneID" id="10601410"/>
    <dbReference type="KEGG" id="aho:Ahos_1911"/>
    <dbReference type="HOGENOM" id="CLU_2299182_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="10888" checksum="231F3E959287438C" modified="2011-06-28" version="1">MFGGGIKVQGKKNINVNEKTTGTFIEYELKLRSDELSLKHTAGVSISESGFNPGGLIGMGISVYKKHEMNKDIQAVKPQVTNEAIAIYNQVMNFIANTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P6</accession>
    <name>F4B7P6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776708.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P6"/>
    <dbReference type="STRING" id="933801.Ahos_1920"/>
    <dbReference type="GeneID" id="10601419"/>
    <dbReference type="KEGG" id="aho:Ahos_1920"/>
    <dbReference type="eggNOG" id="arCOG03859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712934_0_0_2"/>
    <dbReference type="OrthoDB" id="21363at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055771">
        <property type="entry name" value="DUF7347"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24038">
        <property type="entry name" value="DUF7347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="393" mass="43210" checksum="68AC60C522F5B417" modified="2011-06-28" version="1">MDIFEAIANETRRKIIKILGEKGKAGFSDLMNELGLDSPSLAFHIRKLGNLITKVNDEYTLTEEGKKAYKIIMEVEGRKGESILEREEENSPQQEITPPQSSNVVEVRDLGFYEITPELVKSLKDNKSRLRIVRVGILKIDDSVNPEDLKNVIDEINEVGIIDCSPKCSIAISPKLSNVGLIGQSFAKALQESLKFPFHIYTTHNKKFTVFDSSIQPPKSCEIEVDGSDLTIVQGEPHLKVECRSLDDVEINLGEKMSLEINGCEAVLSLPNLEKLDVEINGGDVNLNLNVKEVEIDADGSDANINITGCEKAEIDADASSVKGKINFPSGGILDIDNNASTVRLTLDLPENVGIINDVRSNISHVELPPIRQGEKKLKIDADVNAGELIIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q0</accession>
    <name>F4B7Q0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776712.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Q0"/>
    <dbReference type="STRING" id="933801.Ahos_1924"/>
    <dbReference type="GeneID" id="10601423"/>
    <dbReference type="KEGG" id="aho:Ahos_1924"/>
    <dbReference type="HOGENOM" id="CLU_1840450_0_0_2"/>
    <dbReference type="OrthoDB" id="40278at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="139" mass="16173" checksum="13E7F824FAC252D7" modified="2011-06-28" version="1">MTSYLFAINGSSLHTLLLSIDEEKSGDTTYFVYSGFLIENEEVSLPLENYYNLPAFSTAYYIALRKRKAYTILKRHYYEGAVPLYLIFRTNDEKKAMEKALKTFKDTLLMMGLGLIFKLPSSYLELLSADYVKLLKKWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R2</accession>
    <name>F4B7R2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enzyme for biosynthesis of extracellular polysaccharides</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776724.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R2"/>
    <dbReference type="STRING" id="933801.Ahos_1936"/>
    <dbReference type="GeneID" id="10601435"/>
    <dbReference type="KEGG" id="aho:Ahos_1936"/>
    <dbReference type="eggNOG" id="arCOG05403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182726_0_0_2"/>
    <dbReference type="OrthoDB" id="8690at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050404">
        <property type="entry name" value="Heme-degrading_MO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34474">
        <property type="entry name" value="SIGNAL TRANSDUCTION PROTEIN TRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34474:SF2">
        <property type="entry name" value="SIGNAL TRANSDUCTION PROTEIN TRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="97" mass="11724" checksum="A00128D4D17E9A19" modified="2011-06-28" version="1">MINVGLYYRVKEGHEKEFEEIFKKVVETLKSANVGFIDGKLYKNVDNPREYMIYSEWKDLDSFRKFMLSREFKNTTDYGKQIIEGRPYHRIFQEINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T9</accession>
    <name>F4B7T9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc-containing ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_1963</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <cofactor evidence="10">
            <name>Zn(2+)</name>
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    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="10 11">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <text evidence="10 11">Binds 1 [4Fe-4S] cluster.</text>
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        <property type="entry name" value="Fd_Zn-bd"/>
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    </dbReference>
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        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
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    </dbReference>
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    <keyword evidence="2 10 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
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        <ligand>
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        <ligand>
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        <location>
            <position position="56"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
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        <location>
            <position position="87"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:49883"/>
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    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        </source>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000068-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="11375" checksum="18DB58BA1351A2D8" modified="2011-06-28" version="1">MGIDPNYRTTRKVVTEIQGIKVYDPYEPPKKLGIWGTIVGVDFDLCIADGSCITACPVNVFQWYDTPGHPASEKKAMPINEQACIFCMACVNVCPVAAIDVKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W7</accession>
    <name>F4B7W7_ACIHW</name>
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        <submittedName>
            <fullName evidence="4">Phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="4 5">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="97"/>
            <end position="221"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="237" mass="27145" checksum="6E52AF280F4C41CD" modified="2011-06-28" version="1">MIKVSKDKEIRNRLLAIDILRELKNSYTYKEMSEIFGIQETLICRYVNGNTVPSDVQSMEIISKVKNKEFLLKFFRNKIKVLEDGYIDSSQLLLYPNLLKLLLELYLTKLMQKDAVDKIFSIATNGVPFATIASLILDKPLLIAKKHKDSINLEYYEESLKETDSVVNNIYLRKDLIKKNDRILIVDDVIKSGKTISSSIKLLQKAGARVVGILVLVGNLDNIKYLKDESIQVIFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z7</accession>
    <name>F4B7Z7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized metal binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0788</name>
    </gene>
    <organism evidence="3 4">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775590.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z7"/>
    <dbReference type="STRING" id="933801.Ahos_0788"/>
    <dbReference type="GeneID" id="10600248"/>
    <dbReference type="KEGG" id="aho:Ahos_0788"/>
    <dbReference type="eggNOG" id="arCOG01120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044517_0_0_2"/>
    <dbReference type="OrthoDB" id="35947at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="31"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="13957" checksum="6F3DADD50F915245" modified="2011-06-28" version="1">MEDLRLLKKAKISVMQGRIVKIRSKQGFLFTYVFLGKDGSKHKDHIVNENYCDCEFFIFNKIYKDKAFCYHILALKIALHREKIITIDVEFNDFLDIIREIRHDGKSLKLRKLIYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B808</accession>
    <name>F4B808_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="10">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hisS</name>
        <name evidence="13" type="ordered locus">Ahos_0799</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775601.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B808"/>
    <dbReference type="STRING" id="933801.Ahos_0799"/>
    <dbReference type="GeneID" id="10600260"/>
    <dbReference type="KEGG" id="aho:Ahos_0799"/>
    <dbReference type="eggNOG" id="arCOG00404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="OrthoDB" id="8659at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="273"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="426" mass="49018" checksum="9B89616787119182" modified="2011-06-28" version="1">MVSYEPLRGMRDYFGEDAEKIRYIEEQFSKIVKKSGYMEAITPILEEFELFALKGGEELRNTMYTFIDKGGREVALRPEFTPSIVRLFLNSMQHLPKPIRLYYIGTVYRYDEPQFGRYREFRQAGIEMLGESSINADLEVLSLLWEFFNKINLTNITIKINNIAIYRELFSILKMSEDQEDHLLHLIDKGNKDSVMEYLSNIADSKILNILREMLYNSISKESDFIELIKDMNDEHSKVLENQISRLFKIYEILAKLNVPIKIDLSLVRGLAYYTGVIFEVTHPSVSFSIAGGGRYDNLVQLYGGPSTPSIGFAIGVERVLSVLQNLKIKDNARKIAIIPLNENVIDYALKVLFILHENDIPAILNTKNISLSKIIPNLLDMNINIVIIIGENEKRENKVSIKDLTTRQQSSISLNDLLPYIRQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B861</accession>
    <name>F4B861_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776800.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B861"/>
    <dbReference type="STRING" id="933801.Ahos_2013"/>
    <dbReference type="GeneID" id="10601512"/>
    <dbReference type="KEGG" id="aho:Ahos_2013"/>
    <dbReference type="eggNOG" id="arCOG01205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159724_0_1_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF1">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="14274" checksum="672E515FCE81B95B" modified="2011-06-28" version="1">MSKDYAEILKMMYEERKEILHNIEKYLKEIKEAVIEKDPDAKVILFGSFIRGNFRPDSDIDILIISEKYGDNPHKYAELVNYIRDKIQHYSLFEFHVVTRRTYENWYKKFIDVYKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B862</accession>
    <name>F4B862_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="5"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="130" mass="15406" checksum="C79E73C6CA95C30C" modified="2011-06-28" version="1">MSFLRDNARQFLEQAKFSMERGFYNLVLFNVEQSIQLNLKFLLYSLTGDFPKTHKISELFKYVINILDNSCNLSNFYEENKDFISIIEFSYISSRYLPQTFSKDDAEKSLRIGEEFSKMIENCLKTMQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B870</accession>
    <name>F4B870_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2022</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776809.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B870"/>
    <dbReference type="STRING" id="933801.Ahos_2022"/>
    <dbReference type="GeneID" id="10601521"/>
    <dbReference type="KEGG" id="aho:Ahos_2022"/>
    <dbReference type="eggNOG" id="arCOG00196">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="281" mass="31334" checksum="2C9B836FCD176885" modified="2011-06-28" version="1">MIFIENLTKSFGGKEVLKGVSFEVKKGSITGFIGPNGAGKTTTIKIISGLIRKTRGVVKVLGEDPWDNPKVHEKMSVIFTNLVYPQENTVEEYLKDIGRIYGTDITELVDEFKLRPHLKKKLSQLSSGLAQRVQLVASLIKNPELIIADEPTANLDPPARIEFYEEVKKLNKQGVTFFISSHILSELEKIITDVVFINEGKVTFQGKVEKALKEAEEEEILLIVNDPEKAIKIIGGKIEGSYIKVKGKVRDIVDRLDDAGVEIISLRRSSLDDAFKKLSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B881</accession>
    <name>F4B881_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plasmid stabilization system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B881"/>
    <dbReference type="KEGG" id="aho:Ahos_2034"/>
    <dbReference type="eggNOG" id="arCOG01665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157019_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10723" checksum="A9E00E7C1B80F5C0" modified="2011-06-28" version="1">MFSKRISNLDELADYLLDSFASDKLVMLILDKVLLLLDNPFKYSREKLGKDKHGNSMLSIEVTGDIRILYSVDPKNCIVFIWEIGSHKKVYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E9</accession>
    <name>F4B8E9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein eL19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl19e</name>
        <name evidence="9" type="ordered locus">Ahos_0862</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775664.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E9"/>
    <dbReference type="STRING" id="933801.Ahos_0862"/>
    <dbReference type="GeneID" id="10600330"/>
    <dbReference type="KEGG" id="aho:Ahos_0862"/>
    <dbReference type="eggNOG" id="arCOG04089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="OrthoDB" id="11624at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000003">
        <property type="entry name" value="50S ribosomal protein L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_eL19_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="8">
        <location>
            <begin position="3"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17754" checksum="6F2FF14A3250FF35" modified="2011-06-28" version="1">MPELYLQKRLAADIAKVGINNVKIPPENIDEVKEALTRADIARLIKEGKIIIEKEKERSNSKVKERRKARRVKGEGRRHGSRKGKKTARFDEHEAWVNKIRKIRKYLKWLRDHEVIDSHLYRQLYIKAKGGSFKSISDVRSVLIQMGKLKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F7</accession>
    <name>F4B8F7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl34e</name>
        <name evidence="6" type="ordered locus">Ahos_0870</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775672.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F7"/>
    <dbReference type="STRING" id="933801.Ahos_0870"/>
    <dbReference type="GeneID" id="10600338"/>
    <dbReference type="KEGG" id="aho:Ahos_0870"/>
    <dbReference type="eggNOG" id="arCOG04168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118652_2_0_2"/>
    <dbReference type="OrthoDB" id="43096at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_eL34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047868">
        <property type="entry name" value="Ribosomal_L34e_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003143">
        <property type="entry name" value="PRK04059.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="87" mass="10116" checksum="B752437A4EE5CEDD" modified="2011-06-28" version="1">MPKPQFRSRSYRKLHIRTPSGESKLHFERRKNKLAHCAICGNPLNAVKTNKVYSFSLTEKRPERPFGGYMCHRCLENLIKLSVRGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K6</accession>
    <name>F4B8K6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pseudomonapepsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_2087</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776873.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="STRING" id="933801.Ahos_2087"/>
    <dbReference type="GeneID" id="10601587"/>
    <dbReference type="KEGG" id="aho:Ahos_2087"/>
    <dbReference type="eggNOG" id="arCOG03665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006754_0_0_2"/>
    <dbReference type="OrthoDB" id="56693at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017001">
        <property type="entry name" value="Pept_S53_physarolisin-II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032623">
        <property type="entry name" value="Peptidase_SSO2181_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1296"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="9">
        <location>
            <begin position="206"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1321" mass="143525" checksum="FBB575325C2A19D8" modified="2011-06-28" version="1">MKSVLVFLLVFIIFLSSVTGGIEIVYSQTAKDCYIAPDITGTPLGKLPSNIPIYINIFLPPKNMNELLLLMQEEYHGQIHLSRQQIINEFGDQQRINEISSYLKSNGFNVVFTTPFSLMAEAPACKVNELFGTQLYLYKNGQEIFYKPYNNPNIPSVLQNVIIGGLTNYTTVETQYLVLGKLVDGVLKPTNSSRQVPLGLNFAYTMYSPQDLEGAYNVTGPEGRNVTVAIIDAWGDPLICQDIQEFDQEFHLPPANLTVVPIGPYEPLFGLFTGWYGEVALDVEAVHTMAPYAHIELVLSHGCSFCDILQAIIYVVSADNAQVVDMSFGAVENEFTASGMYAYYRGVPLVNYPLVNYYFELGSAEGITFIAASGDRGAYEGTYTTFGGVVFPSSSPFVLSVGGTSLYPQITSGYIATMNSTAVYGYENAWSVLPQYECLGTSTVASGGGYSTFFPAPYWQASITKSDTRATPDVSADANPYTGFITIVLGTKEVIGGTSLSTQLWGGVIADIDSYIGHPLGLVAPILYSIYENSTLYQEAFHEVTQGFNGKYLAHAGYNLVTGLGTPNVGVLEEVIKEYLSSHPELEISLSTCEPGFTMPWYCYCTTFHIIAEISYPNGTIVTNGSFSAYIYTLKGFLASVPLTYNGTYWVGNFTISKGDPANCWTIVVNGTAGGLFGISQVDVDVGYSFNIISPPEGVIPVNAPVSFCVCIYNPQGQPVTNNTVTMTMMYHGKVVSVTPLVYTGRYGDYSGTLILLYPQPQGTYIIEANSTYGCAYEWEYFGLVTYGAVIPPINDGMTSAYPGENITVLGYIFNPCLQGIFTAKAYAELVSLNGQVIEKVPMTLAPDTTILGVYNLFGYHIANITIPSNVTPGYYKVIVTSCLPAVFGPSYGNFTTFIYISNSGLSYQVRSVSEVYEGQYIKVFANITYSNGTEVKYGEFTAGFIPTELNFEQLLVEFYAGVPMQYNSTLGEWETIYQIPSILSEQCTIYEGAPTQSLSGFWDVVIEGTSSSGNNVVTNYSYFNVLPYTYIGSKIITPQNASKVPLIIFNGTNYILQGIYSPSLTIENLHGLIIECSEVGSVYVVNSTLVVEKSQVTLIHATSSSLCLIQDMIGHVTNASPLNLVKSNATLISTVIEDSKYAFNITCSKVNLEGVYYKNVTEVSNLPTPTIVSYSPVNVTTPSANIQFNISGKNLKVIGVYMDGKPISFSSTSTSSGIQVTIPFNASVEPSGAYYVTIEVCDGLKYNLSAIIFNSYHEVEEAKVSASLSSNISSVSTSLSKEISSVSASSTDGEILGSIGIVLGLIAILLFVFRGRGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q3</accession>
    <name>F4B8Q3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lysine biosynthesis enzyme LysX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0887</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RimK family. LysX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775689.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q3"/>
    <dbReference type="STRING" id="933801.Ahos_0887"/>
    <dbReference type="GeneID" id="10600358"/>
    <dbReference type="KEGG" id="aho:Ahos_0887"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_1_2"/>
    <dbReference type="OrthoDB" id="33241at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000025">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000058">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054562">
        <property type="entry name" value="LysX/ArgX_preATP_grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011870">
        <property type="entry name" value="LysX_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02144">
        <property type="entry name" value="LysX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22626">
        <property type="entry name" value="LysX_preATP_grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="93"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="283" mass="32658" checksum="6BFAD38CEF254F2C" modified="2011-06-28" version="1">MIVGVTYDLLRWEERNLIQEATKLGHKVIPIYTKDFYHLFSEDNQFQNLDVVLQRNTSHQRALSTSLIFETLGYNVINDSRTLINCENKLITTAILSRHNIKVPKTAVAFSKDKALEVASKLGYPAVIKPIEGSWGRMVAKAVDEDTLRSFLEYQDYTSVGFKNVYYIQEFVRKPDRDIRIFVIGDEAPVGIYRVNTKNWRTNTALGAIAEPLKIDDELKDLALKVKEVIGGFFLGIDIFEDKERGYLVDEVNGVPEFKNTVRVNQFNLSEYLIRKIEEWIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R2</accession>
    <name>F4B8R2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0896</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775698.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R2"/>
    <dbReference type="STRING" id="933801.Ahos_0896"/>
    <dbReference type="GeneID" id="10600368"/>
    <dbReference type="KEGG" id="aho:Ahos_0896"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999709_0_0_2"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17777">
        <property type="entry name" value="CBS_arch_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17778">
        <property type="entry name" value="CBS_arch_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF6">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="80"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="143"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="214"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="276" mass="30987" checksum="48BDA7D3F58A39EC" modified="2011-06-28" version="1">MIVKTLEVLRPPIISIEDSLMQAFKRINERGIGRVIIANEKVEGILSTRDLLSVYLSFCPQSCSQGDLYKMSNMKASLYMTPNPAVVNEKDDILEAITIMVTRNFGSLPVVDDLGRPTGIVTEREMLLSFQDLEVLFPVSMFMSKKVTTINKDVDLVQATRQMLHRGFRRLPVVDEEGKVIGIVTAADCIKAASKSVEKLDPDYFFSKKVTDIMSTPPISIEEDRSINEAAATLIEKNIGSLLILDDESRPKGIITERDLLIALHYQLHLQFLRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S8</accession>
    <name>F4B8S8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775714.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S8"/>
    <dbReference type="STRING" id="933801.Ahos_0912"/>
    <dbReference type="GeneID" id="10600384"/>
    <dbReference type="KEGG" id="aho:Ahos_0912"/>
    <dbReference type="eggNOG" id="arCOG01630">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_3_2"/>
    <dbReference type="OrthoDB" id="371655at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RutC-like"/>
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    </dbReference>
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        <property type="entry name" value="RidA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2-IMINOBUTANOATE_2-IMINOPROPANOATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="13675" checksum="6DA8B1D8ABD6B9CE" modified="2011-06-28" version="1">MKEIIYTEKAPKPIGPYSQAVKLGNLIFVSGQIPLDPKSNNVVNGGIKEQTAQVLENIKAILEASGSGLDKVLMSFVYLKSMNDFQGFNEVYSMYFKDNPPARVTVEVSKLPKDVLIEIAVIAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W6</accession>
    <name>F4B8W6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methylenetetrahydrofolate reductase (NAD(P)H)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775752.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W6"/>
    <dbReference type="STRING" id="933801.Ahos_0950"/>
    <dbReference type="GeneID" id="10600422"/>
    <dbReference type="KEGG" id="aho:Ahos_0950"/>
    <dbReference type="eggNOG" id="arCOG00475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098912_0_0_2"/>
    <dbReference type="OrthoDB" id="28177at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38755">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38755:SF1">
        <property type="entry name" value="METHYLENE-TETRAHYDROFOLATE REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25077" checksum="1D614CBEEB5FBFF5" modified="2011-06-28" version="1">MQLLAEVHPKTKIEKIKKEIQDLSSFDGFDIPDSPLGLPSVKPTSIAALIREKYEHKRIIINQRLLDVNELYVHSLSLTAKAFNLNIVFTKGDKPKIGKEVGYLSSEEAVNIAKGYGVSSGMMISLRKNRSEILARLESNADFYLALHFSGVESIKGLEESFQKIIPYIIVKTEKNKEILSNISQPSFEGSKVRDLLYELESVGIKSVLISSPGDLNYLANFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X9</accession>
    <name>F4B8X9_ACIHW</name>
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        <recommendedName>
            <fullName evidence="3">VapB-type antitoxin</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776917.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8X9"/>
    <dbReference type="STRING" id="933801.Ahos_2131"/>
    <dbReference type="GeneID" id="58788777"/>
    <dbReference type="KEGG" id="aho:Ahos_2131"/>
    <dbReference type="eggNOG" id="arCOG07723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075110_0_0_2"/>
    <dbReference type="OrthoDB" id="40882at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="52" mass="6069" checksum="E0390E7FCD293DE6" modified="2011-06-28" version="1">MAILKVSEDVKEELLKLANELQDKRGGKRDVTINSVIEFLIEFYREKSKENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z0</accession>
    <name>F4B8Z0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776928.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z0"/>
    <dbReference type="STRING" id="933801.Ahos_2142"/>
    <dbReference type="GeneID" id="10601645"/>
    <dbReference type="KEGG" id="aho:Ahos_2142"/>
    <dbReference type="eggNOG" id="arCOG00040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080904_0_0_2"/>
    <dbReference type="OrthoDB" id="4952at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="12"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="206" mass="23860" checksum="DD3C4A3A9D2901BB" modified="2011-06-28" version="1">MPKIPVKVVKWEEIADLSFRLADILKKENFNPDVIIAIARGGLVPARLVADAMGVYDVLSLKVEHWIVTASHTPEAKIKYPYKVDLQGRKVLIIDDITDTGDSLILAEKHVKENFNAGEIKTATLQYIKGSKYVPNFYADIITDWTWFMYPWNYWEDEINLLKKVMDEMKTTDIAKLKEGFKESYNIDPPIPLEKIMEEMKRRKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B900</accession>
    <name>F4B900_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776938.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B900"/>
    <dbReference type="STRING" id="933801.Ahos_2152"/>
    <dbReference type="GeneID" id="10601655"/>
    <dbReference type="KEGG" id="aho:Ahos_2152"/>
    <dbReference type="eggNOG" id="arCOG07196">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197256_0_0_2"/>
    <dbReference type="OrthoDB" id="34311at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="80" mass="9385" checksum="28DED4DA06A5D84E" modified="2011-06-28" version="1">MIVEVIYNNITTEMLEIIRKIRRKALASEIIFYKGKKNVIIADNMKIWEESDKSKDPLEEIYDAKIIELVKQIGKLPSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B930</accession>
    <name>F4B930_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TGS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776968.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B930"/>
    <dbReference type="STRING" id="933801.Ahos_2182"/>
    <dbReference type="GeneID" id="10601685"/>
    <dbReference type="KEGG" id="aho:Ahos_2182"/>
    <dbReference type="eggNOG" id="arCOG00358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_1_2"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01666">
        <property type="entry name" value="TGS_DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000016">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="3">
        <location>
            <begin position="279"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="354" mass="40243" checksum="B767696D35706CAA" modified="2011-06-28" version="1">MVTNLPAEAKAKWLKVMDAKTPEEKIKAIQDFLSSVPKHKGTENLVYWARRRLAELREESERQRKKKSGSFSFFVEKEGAGQIIVLGREELKNPLVRKITNVKQDPKDFPIPAMTYYEDVGIQLVNPPPVILDSKLIVSKIIGLIRNTDGLLFVVEDKNEFLKFKEFLESNNILLGKPKGKVIIEKLRSGKEGIRIILLGKLVNTDENEIRKYLQDFGLKSAIVKIIGEVSLDDVEKSIFETVSYKPAVVVSLNPFDYPAIPVITKIDNLPKYLFDSLDIIRVYTKEPGEEPSKDPLILKRGSTVLDVAKKLHSSLYENFKYARIWGKSAKFPGQKVGEDHVLEDKDIVEIHTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B936</accession>
    <name>F4B936_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2188</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776974.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B936"/>
    <dbReference type="STRING" id="933801.Ahos_2188"/>
    <dbReference type="GeneID" id="10601691"/>
    <dbReference type="KEGG" id="aho:Ahos_2188"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_2"/>
    <dbReference type="OrthoDB" id="43786at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000003">
        <property type="entry name" value="Phosphomethylpyrimidine kinase ThiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="6">
        <location>
            <begin position="271"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="447" mass="50378" checksum="EE9210F0CAD51D1B" modified="2011-06-28" version="1">MIKNVALTVAGIDTGNGAGAETDIKVFEMLGVHGVMAITAITAQNTKGISDIEVVKPDFLRKQIKVVKSDFDIKSVKIGMIYNKDQFKVVNEELSGYKIITDPVVYAKDGTQLINDVDEYKKLILRISTIITPNAVEASILSGIKIDTIKDAEEAIKKLYEEYGVPFVIVKGGHMRGEYSFDILFDGKEFYEIGYPRLDKKDTHGTGSVFASVIAGKIAKGEEVINAFREARKILQNAIDYGLEIGHGIGPIDPLVYNEKKSQKYQVIEDMTKIGDFIENEEKFWKLIPEVQSNVAHSISPQYVEDLNDIATFRGRIIRRWDRKVIVGYPAVFGNPTHTARLLLAIILNYRIGNTLINIRYDEKILSAFKSLGYEIIEINRELEPQHGEGKTMQWIIEYIKTQYGIKNNLPNVIFDKGMKGKEAMIRLWTNSADEMIESLRLVCKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B944</accession>
    <name>F4B944_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type Na+ efflux pump permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B944"/>
    <dbReference type="STRING" id="933801.Ahos_2196"/>
    <dbReference type="KEGG" id="aho:Ahos_2196"/>
    <dbReference type="eggNOG" id="arCOG01462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055390_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43471:SF3">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN NATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="17"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="390" mass="43223" checksum="D49F2939DF3E9123" modified="2011-06-28" version="1">MKDLIKKEWTDVKRDRKLLLGSILLPLILLPLIGVILFAAIVSQPPVVDIINENYDNIKYVKELCSYIQGNGGIVYINSTQPADVEVVFPSCFYENISDINRTAIVYISYVISSRSSALQLVENGLYNILYNTSIQRIKYLENMSHTEVSPSIIRDPLEVTLLYKLPTGEQASYQQSQFSQLARIVAIILFPAATPVIFFVSDSIMGEKERKTLESLLASPISSRAFIFSKLIISIILGLISSIGDLIGLVAFSLFAPYIIGESITFSATFAILVIIVYLAMILLTASMSIIVLLLLGGSSRNVQIINFIITSFGMIASFSSLFLNFGDLSYPLSLILGIPYVQLVASIMFYVFGLIEESIFSISITLLASIFLLLLASRFLDSERLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B962</accession>
    <name>F4B962_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775771.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B962"/>
    <dbReference type="STRING" id="933801.Ahos_0969"/>
    <dbReference type="GeneID" id="58788751"/>
    <dbReference type="KEGG" id="aho:Ahos_0969"/>
    <dbReference type="eggNOG" id="arCOG08342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075109_0_0_2"/>
    <dbReference type="OrthoDB" id="34427at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="52" mass="5879" checksum="EB35059A17A3F48E" modified="2011-06-28" version="1">MNLIEEILMIIGTIMLPYGLYDIAKGEGDKQVKILLIAISLTLFIVEFLLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B974</accession>
    <name>F4B974_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_0981</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775783.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B974"/>
    <dbReference type="STRING" id="933801.Ahos_0981"/>
    <dbReference type="GeneID" id="10600453"/>
    <dbReference type="KEGG" id="aho:Ahos_0981"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_2_2"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050106">
        <property type="entry name" value="HistidinolP_aminotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643:SF6">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43643">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="12">
        <location>
            <begin position="119"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="322" mass="37814" checksum="D774EB347457E3DE" modified="2011-06-28" version="1">MKHGGVRWINGRPEKLRDFSVNLNPIYPDFIEELINEAIKEKVYLYYPDNYEKLRENIAEIYDVDEDYIGVFNGASEVINLLEPRVVPEPNYVEYKRSYIYFAEERENYFIYRLQGNKVIISNPNNPTGAKIDLKEIKKFLEEGKDLVVDESFADISLVDSAKKFVKDYDNLLIVSTFTKSLSIPGLRIGFTIGKRSKELEKNSPPWRINSVAYYVLSNANPKEIKDFFRKSRDLVASLLENFRIREERRRVTVYKSFAPYVLVRFPINVRFINSYLRSKGFLIRDCSNFVGLNEFYGRISLRKDYQELVNEIDEFLSNSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A6</accession>
    <name>F4B9A6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775815.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A6"/>
    <dbReference type="STRING" id="933801.Ahos_1013"/>
    <dbReference type="GeneID" id="10600487"/>
    <dbReference type="KEGG" id="aho:Ahos_1013"/>
    <dbReference type="eggNOG" id="arCOG05431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782162_0_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="208"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="352" mass="40315" checksum="C2DFB89B08A98CB9" modified="2011-06-28" version="1">MQIAVRLLWNSAVPKMAIEEARNTNWKLIIYRDAGGNYKLDGVNYVILRKRGEKGFFTPLFSFITSIYAKHRGKEATVDLDLILRATKIKGPTLYHDQFAGITGYIRKLRYGEDYAVYLHETSLPLRGAKYFLPKKIEEKVLRNAKSIITNSMWNRKTLEEYGIKSEVIYPGCYPVSKLPESRERIVLAVSTWDSGRKPEIYGEIAKRIKGKMVMAGSWAREDTLKEFLREYGNYVTVTGKISEEKLVELYSKASVLVRFGFNERGPGLGVIEAMAYGVPVIVNEGLGSKELIKQGENGFVVRDWEEAIDRINETLENWHKMSVNAWNTAKELSWGNHAEKLKEFLCQTFEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D5</accession>
    <name>F4B9D5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776996.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D5"/>
    <dbReference type="STRING" id="933801.Ahos_2210"/>
    <dbReference type="GeneID" id="10601715"/>
    <dbReference type="KEGG" id="aho:Ahos_2210"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_2"/>
    <dbReference type="OrthoDB" id="27340at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="16"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="323" mass="35639" checksum="A9C980E19747871D" modified="2011-06-28" version="1">MSLLPKTADIKPGEKFDVIIIGIGPAAYGAALYAGRYMLKTLVIGETPGGQLTEAGEVDDYLGLIGIQAQDMIKTFNAHIEKYNIPVILDTVESFRREGDEYIVKTKRKGEFRATTLIVAVGVKRRKLNVPGEAEFTGRGVSYCSICDAPLFRKKVVAVVGGGDSALEGAELLSRYATKVYLIHRRDQFKAQPYYVELVKQKQNVEFILNSVVKEIKGEKIVKSVIVENLVTKEVKEIPVNGVFVEIGFEPPTDFARVNNLEVDSHGYIKVDEWMRTNLPGVFAAGDCTGMWLGFRQIITATAQGAVAAHSVFTYLNEKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D7</accession>
    <name>F4B9D7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AAA ATPase, CDC48 subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776998.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D7"/>
    <dbReference type="STRING" id="933801.Ahos_2212"/>
    <dbReference type="GeneID" id="10601717"/>
    <dbReference type="KEGG" id="aho:Ahos_2212"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_3_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000661">
        <property type="entry name" value="calmodulin-interacting protein 111 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="94"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="378"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="605" mass="68465" checksum="DF0503D37BAD4AFA" modified="2011-06-28" version="1">MNLSYTYEIIILFILLSIIGIILMGIFKRFTTNFMTSDKATQLQLKGKKKGEDEERKISWNDIGGYEDVKKEIIEYVEFPLKNKELALKYGLRPPKGILLFGPPGCGKTLMMRALASDAKLNFIYVNVSDIMSKWYGESEARLRELFANARKNAPCLLFFDEIDTIGTKRESHTGDSVTPRLLSLMLSEIDGLNSEDGVIIVGSTNVPQLLDKALLRAGRFDKLIYVGPPNKEARKEILKIHCKNKPLAEDVDLDKIADMTERYSGADLANICQEAARKAAIEAMQTGKDVKISMKDFMEIIQRYKPSITLQMIEDFEKFRLDFERRSRKGEDLESEIEDKITLDDIGGYTKVKTELKELLELQLKYSKLMEQMRVPPIRGILLYGPPGVGKTMMAKALAKTLDVKLISVSVAEIMYKGYEGAVSILKEVFNRARENKPSIILLDELDAIASKRSQKDSTDSSKIVNQLLTEMDGIRNLKEVVVIGTTNRVKTIDPALLRPGRFDIIVHMDLPNEEERLDILQKYIGKDVCERVDCREIAKRTEGYTGADLAAVAREAKIRVLKDIIRGNKDRVITKEDIEIALNKIKPSIKNRKKKNSGAQNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E0</accession>
    <name>F4B9E0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777001.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9E0"/>
    <dbReference type="STRING" id="933801.Ahos_2215"/>
    <dbReference type="GeneID" id="10601720"/>
    <dbReference type="KEGG" id="aho:Ahos_2215"/>
    <dbReference type="eggNOG" id="arCOG00040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080904_1_2_2"/>
    <dbReference type="OrthoDB" id="4952at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="9"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="173" mass="20186" checksum="8969E977B379A16A" modified="2011-06-28" version="1">MVEYFIPSWDDIEDSIFNVSEKIIKDYNPDVIVAILTGGVIPAKLISDIIGIKNIKYIEIKFYKEVGKTQPKPTLKAIYVDDLENKNVLIVDDVSDTGETLSAVSRVIELFNPQNIRSATIYVKPWSKKYPDYYDKVVDKWIVFPWDKWEAVREHNEIPVKNKDRFLKSFIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E5</accession>
    <name>F4B9E5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein synthesis factor GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777006.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9E5"/>
    <dbReference type="STRING" id="933801.Ahos_2220"/>
    <dbReference type="GeneID" id="10601725"/>
    <dbReference type="KEGG" id="aho:Ahos_2220"/>
    <dbReference type="eggNOG" id="arCOG01562">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012821_2_0_2"/>
    <dbReference type="OrthoDB" id="30874at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04165">
        <property type="entry name" value="GTPBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035531">
        <property type="entry name" value="GTPBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="109"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="523" mass="58173" checksum="48E7A96407E13B0B" modified="2011-06-28" version="1">MKLPRENDIGKIEYKLILSDVSEERLQELATQMKYRIEEGGGEAIYVIGVSDDGDVIGLNKEDLEHTIDVLEKIAKMINSKIVHKRIVEIRKDKYVADLLIRLHKSDLPIQVNVAVMGHINAGKSTITGTLILGKLDDGNGALRAMIARYLHEVISGRTSSITLRILGFDDEGIIVNHSCRDPLDEAEVTIKSSKIVRLIDLGGHERYLRTTLKGLMGYETDYVMLVVGSDDGLSIMGKEHLAVASVLKYPVFIVITKIDKFPKERVNQIINDIRNVLRIPGINRFVMEVNDEDDLLNAIIGVKTGRVVPIFKVSNVSGEGIGLLTRFLYLLPPRKIITQSNDTLVYIDEIYNVSGVGPVVLGSVVRGKVHPNDTVYIGPVDGGEFLQAKVKSIQINRVFVDEANQGSIATFAIQGVNREVLRKGMILAENNKPKATQSFTAKVIVLHHPTTIKEGYVATLHLYTIRQAVKFEKIARGLLRTGESSEVELKFLYRPEYIEKGQIFIFREGRTRGLGIVTGINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E8</accession>
    <name>F4B9E8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777009.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9E8"/>
    <dbReference type="STRING" id="933801.Ahos_2223"/>
    <dbReference type="GeneID" id="10601728"/>
    <dbReference type="KEGG" id="aho:Ahos_2223"/>
    <dbReference type="eggNOG" id="arCOG01873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061980_1_1_2"/>
    <dbReference type="OrthoDB" id="28434at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04182">
        <property type="entry name" value="GT_2_like_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43777">
        <property type="entry name" value="MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43777:SF1">
        <property type="entry name" value="MOLYBDENUM COFACTOR CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="185" mass="20820" checksum="8909CE0EE9433EC7" modified="2011-06-28" version="1">MKIGAVILAAGESKRFGKNKLLEKINGKSIIENVLENVNIERVIIVGKYAEELLPHLKNEIIIYNPKWKEGISSSIKLGLRFFQNYDGVLIVLGDMPFVKREDIHKIINAFNPDCNAVIPTYKGNWGNPVLLSRKIFDKVMTITGDEGAKKILKSTENLCFVECDYGVIIDIDTVNDLLTFEKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F6</accession>
    <name>F4B9F6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777017.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F6"/>
    <dbReference type="STRING" id="933801.Ahos_2231"/>
    <dbReference type="GeneID" id="10601736"/>
    <dbReference type="KEGG" id="aho:Ahos_2231"/>
    <dbReference type="eggNOG" id="arCOG00695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741058_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="374" mass="41456" checksum="CA273E92A40C6B8D" modified="2011-06-28" version="1">MKILIKAGIAFLKDSSPVPNAYIGVNDGKIEAISKRELEEYDDAELIVGGFDRLVSPGFVTTQSFIQLYPFRYRIFSGKTNPNDLISTMTSKDAYYFSLLGAYHLLRSGITTVVVTEPFVEQAARAVKTVGLRPIVSAEIGCNWIKGDWKKNFESLYSKWISKDESGIVLKLCDEDEAEEAMAISNEYKLPILVDRNVNLERINNISPYTIALGGGSRKDLEKIRKNNLNLAFTPSLEVCKFTLGAYKPSISIDLTPKFDIRNEMGIATSRLLLTAEEAFKAVTDWGYSQLKIGGGISVGNTTDILIFEVNEPPSYPIDKEAPYESLIYSSYSLETVIINGEAVLDGGVPLNVGTKDIEEANRKVEEIGKMEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H3</accession>
    <name>F4B9H3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S-layer-like domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777034.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H3"/>
    <dbReference type="STRING" id="933801.Ahos_2248"/>
    <dbReference type="GeneID" id="10601754"/>
    <dbReference type="KEGG" id="aho:Ahos_2248"/>
    <dbReference type="eggNOG" id="arCOG02089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_435231_0_0_2"/>
    <dbReference type="OrthoDB" id="56770at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902:SF3">
        <property type="entry name" value="NPCBM-ASSOCIATED, NEW3 DOMAIN OF ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35902">
        <property type="entry name" value="S-LAYER DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="628" mass="68108" checksum="E559521CA6C79F82" modified="2011-06-28" version="1">MKYVTTIMIIMILLISFIPLMGHSEVFYNISGYAKVKGLIAPGESEVPITFTLVNTGGTLYNVNITPVSVYPFEVYSGYYNSTNIVNIPELQTDQEINVTFIYNIAQSARDGIYKIQLKISSAELNKTVCFTVPILGYVQISAQSTWGTPSSPMIVTAGESDVPLTIILINQGNVIATNVSLYLPSTYPVDFLEKNIKIGYLPVGEEIPITVYASIFNNATTGVFYVPIKVKYFISSCCIVNLPVIITGYENFSISTIWGTTSSPITASPGSTQLPLTFIVKNLGDITATNVSIQIVKSFPLILSQNTIYIGIVPAGEYNLNTITASVYPNATPGIYYIKAIIHYFDTSSTEYIPVLISAPQISLNAITIPPQIFPGYFDVRVEAIILNHGIGIAQNAYVYLKSPFEVISQNNISLGAISPGVPINATFLINVPNSTKPGEYNLTFIIKYDGGEIEKTFNITVYNKANIIIEKIIYPTINPGSSAVPITIILKNVGNATACNVKVILGSSNVITPHVSTSNPLEALTASEQEIGDLKPGEEINVTYIVDISSGASPGYYPISIILVWNQTGSIYPLAQVDESTIYVSPSLLSQITSYDIVSIPLIYIVIIAVIIIILVAIAVARRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K1</accession>
    <name>F4B9K1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hydroxybutyryl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2276</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777062.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K1"/>
    <dbReference type="STRING" id="933801.Ahos_2276"/>
    <dbReference type="GeneID" id="10601782"/>
    <dbReference type="KEGG" id="aho:Ahos_2276"/>
    <dbReference type="eggNOG" id="arCOG00249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_2"/>
    <dbReference type="OrthoDB" id="39812at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="3">
        <location>
            <begin position="3"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="183"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="297"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="388" mass="43460" checksum="68799B339B158C41" modified="2011-06-28" version="1">MKFAVVGSGTMGHGIAEVLAIYGHQVKLIDISWDILNRAKQRMEESLRKFYEKGTVKEDPSVILSRIEMSTSYDVAKDIDFAIEAVPEILDLKRKVFQSLDEIAPKEAILATNTSSIPISEIAEFTSRKDKVIGMHFFNPPQIMKLVEVIPSKYTSEDTANKTVELARSLGKVPVKLRIEVPGFIGNRIFLRLMQEACREVESGEASIEEVDSCARNKIGLPMGIFELSDYVGLDIDVDLWENIVKRGTSDVPCKLFKEKVARKELGVKSGKGFYEYPGNKYLKVKLPATSKVDPARLLSLAVNEAAWLVENNIVKPSEVDDVMKYGYNFPKGLLEIADELGIDNILRNLEDIYNKGYSAYKPQDLLVKMVKEGKVGKKSGEGFYKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M3</accession>
    <name>F4B9M3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histone deacetylase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M3"/>
    <dbReference type="STRING" id="933801.Ahos_1055"/>
    <dbReference type="KEGG" id="aho:Ahos_1055"/>
    <dbReference type="eggNOG" id="arCOG00324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10001">
        <property type="entry name" value="HDAC_classII_APAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="322" mass="36186" checksum="4D27C61AC790B0E7" modified="2011-06-28" version="1">MYVVYSDKYKEHYSSMHHVENPDRLTRALSSLKGEKIVEPIKVDDPQIVHSEDYVEKIRNIKGEEWIDADTYANDKTYETALYALGGALKAFELQGFALVRPPGHHAGKNGRAFNAPTLGFCIFNNVAYVVRKKALKHVAIIDFDVHYGNGTQEIFYDDPEVLHIDIHQDPKTIFPGTGFPEMVGKGEAEGTKVNLLIPPRGSDDLYEELFPLIQSILEDFKPAYIIFSAGFDGFKGDGLSSLNLTEYTFYNLGSLGKGKYSSGVLEGGYGIGLERGLPAFIKGLKGEATDYVKEKSPDSVKSRFFDYLEKEKEILRNYWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P0</accession>
    <name>F4B9P0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protease HtpX homolog</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">htpX</name>
        <name evidence="13" type="ordered locus">Ahos_1072</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775872.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P0"/>
    <dbReference type="STRING" id="933801.Ahos_1072"/>
    <dbReference type="GeneID" id="10600546"/>
    <dbReference type="KEGG" id="aho:Ahos_1072"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_4_1_2"/>
    <dbReference type="OrthoDB" id="28389at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07338">
        <property type="entry name" value="M48B_HtpX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002322">
        <property type="entry name" value="PRK01265.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="12">
        <location>
            <begin position="83"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="327" mass="36931" checksum="F18CEC6A1FB4CB33" modified="2011-06-28" version="1">MNLTVLKLRLGMILTSLGIFLLGFALVFAVISYFYGISFAPAIIIGILSFLIALNIIQWLFGPYLINAMYKAVEVTPNDPKYGWLIDVVNEVAYYNRISPPPKVYIADVPFPNAFAYGSPIAGRRVAITLPLLKILNKEEIKAVLGHELGHLRHKDVELLMAIGLIPALIFWIGYSLMWSGMLGGGGEGRNNNAGSLWLVGIGLLAVSYIFQFFLLYLNRMREAYADVNSAETVPGGAENLQRALAKITLSVDPETIERYRRKHNNNIANMLFFAPTEVQEDIPENNVDELIEYWKREKVPWYADFFSDHPHPAKRIQLLEKLKYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P3</accession>
    <name>F4B9P3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Teichoic acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775875.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P3"/>
    <dbReference type="STRING" id="933801.Ahos_1075"/>
    <dbReference type="GeneID" id="10600549"/>
    <dbReference type="KEGG" id="aho:Ahos_1075"/>
    <dbReference type="eggNOG" id="arCOG07183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_556249_0_0_2"/>
    <dbReference type="OrthoDB" id="43802at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="498" mass="55790" checksum="A2C5DEF34A315C8C" modified="2011-06-28" version="1">MLKTKKSPGFVRIGLFNLLVKTAVAPISFLFSFLVVRYLSSISIETFGVWQYIYVLITGYFTIPADLFSSITSRYSAEGKNVGGIIIINALAGSISSFAYFLLIPTFMHSAGYNQPIYFYTAILLIVLIYLNRISGSIAIGRSPRLTAISASGFQIVRLLSAIIMMFYLNLSINAVIFAYSLGYLVQIIFNLTFVNARLGIDFKVALSAVRKSIVFIMNYVQLMIEATLVWVVVFIVNSAIPVSYFESALIISNLVGWSQAATDGLILKLQESRDPKLIEIAIKFFFTTGGVFLLIIFVDGERLLYVLRPEYVSSIYALIVLSFSTFIRSAYNIFYRAIYMADESLAVESKGEFRGYTAKLTTRNVIISVMGVSSSLFLLYLFKYGNVYKISPILAVIISLALLVNSLGMFFSSYSISKRIYGFNFPVKEVIVSLVLSVISSLPFIGLSKVRVIEQLIIMLEMTSLALTLYIALSYIFNPYARDLLHSIFREIKNFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q5</accession>
    <name>F4B9Q5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
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        <property type="protein sequence ID" value="AEE93971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="933801.Ahos_1087"/>
    <dbReference type="GeneID" id="10600561"/>
    <dbReference type="KEGG" id="aho:Ahos_1087"/>
    <dbReference type="eggNOG" id="arCOG02053">
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        <property type="component" value="Chromosome"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
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        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="3">
        <location>
            <begin position="2"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="130" mass="14392" checksum="7468E15FDF7D98F0" modified="2011-06-28" version="1">MKVVLAYDGSDYSKKAVFFALRFLKKEDEIHLVTVIREAPKSPEQVIIDSEEKAKRSIDEIKSELEGYNVKEKILESNDVADSLIEYCKEIGCDLIVTGSRGLTGLKKVILGSVSSALVSKSPYPVLVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T9</accession>
    <name>F4B9T9_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1121</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775921.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T9"/>
    <dbReference type="STRING" id="933801.Ahos_1121"/>
    <dbReference type="GeneID" id="10600595"/>
    <dbReference type="KEGG" id="aho:Ahos_1121"/>
    <dbReference type="eggNOG" id="arCOG07225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160443_0_0_2"/>
    <dbReference type="OrthoDB" id="34414at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14655" checksum="23AEB620BDA3C8B2" modified="2011-06-28" version="1">MRLSEIVTKFKLSEESEIEVKDNIEFEEIDVDIGTRVLLTNGKRRRIADLGILSIIYRNCSKEFVKDYLDLSHSLEYIHDKYGVYTELEYLAINCESFVKDKDVLATIKELKAYILSRENKQHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V7</accession>
    <name>F4B9V7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777088.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V7"/>
    <dbReference type="STRING" id="933801.Ahos_2302"/>
    <dbReference type="GeneID" id="10601810"/>
    <dbReference type="KEGG" id="aho:Ahos_2302"/>
    <dbReference type="eggNOG" id="arCOG05886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127942_0_0_2"/>
    <dbReference type="OrthoDB" id="42938at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="108" mass="12047" checksum="B1E28BDAAFC0304D" modified="2011-06-28" version="1">MIIFYAVGEKDRAKELVRIITKTRWKTISKHAIKISSSSIGPSIVIFKPTLAGLAVALWLKSKAEELGMTTSVGWFTPITNVPPQVEDAIKTDLNKILMKRLEVPWSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V8</accession>
    <name>F4B9V8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Paired CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777089.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V8"/>
    <dbReference type="STRING" id="933801.Ahos_2303"/>
    <dbReference type="GeneID" id="10601811"/>
    <dbReference type="KEGG" id="aho:Ahos_2303"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_1_2"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09836">
        <property type="entry name" value="CBS_pair_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="72"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="164" mass="17660" checksum="B79F4E7FEE185360" modified="2011-06-28" version="1">MATKVSQIATTKVYVVKPNVTIAEAAKEMKEHNLGSLVVIDSQNRVVGIITERDVVRAVSNRDIDGPVEKYMTKDVKGVTEDTSVTDALDVMLNNGFRHLPIIKSDGKLYGIVSIRDLARALLDVHTMQFGKPAEEVKGSGVVCPVCGMEIDEYGYCGCGTGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y8</accession>
    <name>F4B9Y8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777119.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y8"/>
    <dbReference type="STRING" id="933801.Ahos_2333"/>
    <dbReference type="GeneID" id="10601841"/>
    <dbReference type="KEGG" id="aho:Ahos_2333"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068832_0_0_2"/>
    <dbReference type="OrthoDB" id="33250at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000017">
        <property type="entry name" value="Endonuclease IV related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="24"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="279" mass="31180" checksum="5CE04C966B1FB066" modified="2011-06-28" version="1">MPKIYLGTAGIPLSAKGKSTIEGVKKVKELGLNAMEVEFVQGVKMSIEKAKELGEVAKTIGVRLSVHAPYYINLCSEEKEKIEASKNRILETATRAEAMGADAIAIHVAFYGKKSKREECLDEVKSSLGEILDKSKELGIVNVKYGIETMAKETAIGTLDEVILLSKELDRQIIPYIDWAHTFARQGGQIDYGEILDRLTKELGLTHINSHFEGLEIRNGKYVDIHRSIVYNTPPFEPLAREILKRDISITLICESPELENDALRMKKILEDLGYKFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA01</accession>
    <name>F4BA01_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA01"/>
    <dbReference type="STRING" id="933801.Ahos_2346"/>
    <dbReference type="KEGG" id="aho:Ahos_2346"/>
    <dbReference type="eggNOG" id="arCOG00001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127893_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF2">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="103" mass="12192" checksum="3491D1E64B115B2C" modified="2011-06-28" version="1">MMVFRGLLQVIIAYIIYRYGDMYGYEIKRKLEEIHGKKLPHGLVYITLKRMVNSDILEIYEKDGKKYYKLSEGGKEFLDNHVKVLQKVRGIIDEIVNYMNSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA17</accession>
    <name>F4BA17_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrI</name>
        <name evidence="10" type="ordered locus">Ahos_1130</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775930.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA17"/>
    <dbReference type="STRING" id="933801.Ahos_1130"/>
    <dbReference type="GeneID" id="10600604"/>
    <dbReference type="KEGG" id="aho:Ahos_1130"/>
    <dbReference type="eggNOG" id="arCOG04229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="OrthoDB" id="7000at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.20">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805:SF1">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit C-terminal" evidence="9">
        <location>
            <begin position="101"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="155" mass="17241" checksum="D459844E1CC09374" modified="2011-06-28" version="1">MKNGLIVSKIKNGTVIDHIPAGRALAVLNILGIKGNEGNRVALVMNVESSKMGKKDIVKIEERELNQREVELIALIAPSATINIIRNYEVAEKRKLNLPEKIEGILKCPNPACITNNDVEARSRFIVISKLPLVLKCDYCETTLTEDEVLRQILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA67</accession>
    <name>F4BA67_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">priL</name>
        <name evidence="9" type="ordered locus">Ahos_1181</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775980.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA67"/>
    <dbReference type="STRING" id="933801.Ahos_1181"/>
    <dbReference type="GeneID" id="10600655"/>
    <dbReference type="KEGG" id="aho:Ahos_1181"/>
    <dbReference type="eggNOG" id="arCOG03013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052778_1_0_2"/>
    <dbReference type="OrthoDB" id="46081at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06560">
        <property type="entry name" value="PriL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140914">
        <property type="entry name" value="PriB N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0639">Primosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="8">
        <location>
            <begin position="209"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="310" mass="35989" checksum="1DBA67994469F0AA" modified="2011-06-28" version="1">MAIVNTEAYPFRVSLEQVLSRYGGLSFYDVISNNGDTIKEAKERINRIIKDEDIKHYKDTKNPQLLFYSVLLILSVLGDKKIAEKVCRKEAELFAEDLEKEDEENLLEMANFLGIKVEKKKIEYHVTKGKVVLNYAIYFIDYLRLTKNLRKNSKFSLSERIIKDGYVFIDRKTLLNLIKEVIYEKMMSLVKPIPLSEIPDTIRDLLLLRKGVTPPCISALFKKDKKSEEELKILTVYMIDIGSSTDSIITMLKNNGIQNPEDFLNHFIKDRKTRYIVYNCTQLKKMNLCVSECGVKNPLQLYFGRLDNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA79</accession>
    <name>F4BA79_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH-quinone oxidoreductase, chain I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1193</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775992.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA79"/>
    <dbReference type="STRING" id="933801.Ahos_1193"/>
    <dbReference type="GeneID" id="10600669"/>
    <dbReference type="KEGG" id="aho:Ahos_1193"/>
    <dbReference type="eggNOG" id="arCOG01543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_4_5_2"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000014">
        <property type="entry name" value="NADH dehydrogenase subunit I (NuoI)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004541">
        <property type="entry name" value="PRK05888.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF35">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="164" mass="18741" checksum="84E729E0A7E73982" modified="2011-06-28" version="1">MAVKEYKKENPFRLFVDHLQSIGTGIKYMVKPQRITLKYPEESLTLPTGYRGIIRLYKDVCIGCTLCAMICPADAMKMMTYQGKKLPTINYGRCVFCGFCVDICPVDALKETGVHDVAFSNRRDLIFDPEKFNKNFDNPPEDKVVKKVRAVIDEEKGIKYVPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L9</accession>
    <name>F4B3L9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_0013</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L9"/>
    <dbReference type="STRING" id="933801.Ahos_0013"/>
    <dbReference type="MEROPS" id="M01.021"/>
    <dbReference type="KEGG" id="aho:Ahos_0013"/>
    <dbReference type="eggNOG" id="arCOG02969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003705_0_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="202"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="485"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE92908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="780" mass="90338" checksum="C8BA6838F205498E" modified="2011-06-28" version="1">MQIDSYDVFIEFNFKGLQYHGIEKIKLSTEDKVVLDAEGVKVTRVRINGKSVDFLSDEKTVTISTGNFSGIIEVEFEGKVRDDLVGMYIAPYDNSYIFTTQFESSHARKFIPCVDNPSYKAEFKFTVKVDKDLDVISNMPPQKIYYEGDKKIIEFLKTPKMSTYLIYMGVGKFEEYYDYSSQPIVIVATVPGKILKAKIPADFARKFIKFYEDYYGIKYQLPKAHFIAIPEFAFGAMENWGAITFRETALLADENSSVRQLRRVAEVIAHELAHQWFGDLVTLKWWNDLWLNESFATFMSYKAVNWLHPDWDYWGEFLYSETAGAMEKDSLHITHPIEVEVKKPEEIEQLFDDISYGKGASILRMIESYMGEEEFRKGISNYLNKFSFSNAEGKDLWNSLEEASGKPISNIMPSWIVQEGYPLITVKVKDNIIKFEQRRFMLDGSTDDKIYMVPLTLEVNGNKKISLLLDSKEKEYNVGEKVNSIKVNLNRAGFYRVYYDDLRILGSMNHLEKFGLINDYFSFLLAGIIPFEEYEKIVQSMMNEESYLPVLELASQLFKLYAINPKKYSSLALQFHESQEKIWRTKTDALGKLTYSNIIENLVQMDYNFALELSKEMANFSSIDPNKKDAVARAYAIVNEDSVFDEILDKYRKEKFDEEKMTYLKAMLSFKKPYLVSNTLSLSLTGEIKKQDIVRILPIVAYNVEAKEAVWSWLKTYMDNIRKYYQGTGILGRVLSDVLPILGIGKEKEVEDYFNKHPMPESEKGIRQGIEKLKIFSRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M4</accession>
    <name>F4B3M4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774830.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M4"/>
    <dbReference type="STRING" id="933801.Ahos_0018"/>
    <dbReference type="GeneID" id="10599458"/>
    <dbReference type="KEGG" id="aho:Ahos_0018"/>
    <dbReference type="eggNOG" id="arCOG07314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269947_0_0_2"/>
    <dbReference type="OrthoDB" id="38965at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="216" mass="25256" checksum="7CDBC53CCA376AF6" modified="2011-06-28" version="1">MWIKEISVKFTCKPQSLNFYCNNRGNVIELTASSSCRYLRLFTKDFRLTFSNGKLFDFNNLSTKKGKDAVTEINSILNSLKPGIVEDLNENSLRYEIPISLLKNFVENLNVESVSPERYLDFNIDYIDYDIGRDFLKNSPRFESERRLKMSLSINNKCLRVIYWLDSSNVETFSSEDCVNWTSNTKIYTVVSNISTFDERYLEIKRFLEKITANNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q1</accession>
    <name>F4B3Q1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2249 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776004.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q1"/>
    <dbReference type="STRING" id="933801.Ahos_1205"/>
    <dbReference type="GeneID" id="10600681"/>
    <dbReference type="KEGG" id="aho:Ahos_1205"/>
    <dbReference type="eggNOG" id="arCOG02074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2713031_0_0_2"/>
    <dbReference type="OrthoDB" id="38377at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8593" checksum="F8D7D6087F5E6B08" modified="2011-06-28" version="1">MQQLDLRNKSCEEFIVELSKYLVSMKAGETITLLAEKDRVLCLHQLLRNAPRYLFNYEDKEDHVVINIKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q8</accession>
    <name>F4B3Q8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14 15">Shikimate kinase</fullName>
            <shortName evidence="14">SK</shortName>
            <ecNumber evidence="4 14 15">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">aroK</name>
        <name evidence="18" type="ordered locus">Ahos_1212</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="4 14 15"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776011.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q8"/>
    <dbReference type="STRING" id="933801.Ahos_1212"/>
    <dbReference type="GeneID" id="10600688"/>
    <dbReference type="KEGG" id="aho:Ahos_1212"/>
    <dbReference type="eggNOG" id="arCOG01025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="OrthoDB" id="9602at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="42"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="17">
        <location>
            <begin position="196"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AEE94095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="28162" checksum="8B8464D661F76646" modified="2011-06-28" version="1">MQAYGGVSIVNAIPSWYGSSMAVNLKVNVEITKGKYNGGSKLISTIITYLKEKFNLEDFDVKINSEIPQESGLKSSSAVSTALIGEVKRKFELDIDVVKYSAILSILAGVSYTGALDDAVSAYYGGVSYTYNKEFKIVKKSEPPSDISIIILPKGGRGKVNLNKLRDYELIFYEIFKLSLSDPITAMKYNGILVGEILGYDLKPVKKALEKGALASGISGNGPSIFAVTKEGEEGPIIDEFNLYGKVILTKAVGLESEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R1</accession>
    <name>F4B3R1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">3-dehydroquinate synthase</fullName>
            <shortName evidence="8">DHQS</shortName>
            <ecNumber evidence="8 9">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aroB</name>
        <name evidence="12" type="ordered locus">Ahos_1215</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="8 9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776014.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R1"/>
    <dbReference type="STRING" id="933801.Ahos_1215"/>
    <dbReference type="GeneID" id="10600691"/>
    <dbReference type="KEGG" id="aho:Ahos_1215"/>
    <dbReference type="eggNOG" id="arCOG00983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001201_0_2_2"/>
    <dbReference type="OrthoDB" id="21407at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050071">
        <property type="entry name" value="Dehydroquinate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030963">
        <property type="entry name" value="DHQ_synth_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43622:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001455">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="10">
        <location>
            <begin position="50"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="11">
        <location>
            <begin position="164"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="54"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="152"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR01357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="345" mass="38125" checksum="9AD0E35220B245E7" modified="2011-06-28" version="1">MRKISEDICCSKVDVLVNTSYKDFLDSLQGKKAIFYSKNLSIDYNGDLNIPIEDGEKAKDVSNVLDLVKSLYDNGFNRGDYVIAIGGGTVLDLVGFVSSIYMRGLNLINIPTTLLGMVDAAIGGKNGVNFGNAKNILGTFYQPSAIIIDLNFIKTLPEEEIRKGLAEVIKYGLVLDKELYDYLAMNDKKILEKDEGALEEIIYKSIKDKLAVVKEDERETKGIRVVLNFGHTIGHAIEAGSNFTIPHGYAISVGMVCEAKIAEEMGYSEEGVVEDTLWILSLYGLPITPEKLPSKYSKELALASVEKDKKIRNDEILMPFPTRIGDYKVVKVPIQTVKGFASQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S2</accession>
    <name>F4B3S2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exosome complex component Csl4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">csl4</name>
        <name evidence="4" type="ordered locus">Ahos_1226</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. Interacts with DnaG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776025.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S2"/>
    <dbReference type="STRING" id="933801.Ahos_1226"/>
    <dbReference type="GeneID" id="10600702"/>
    <dbReference type="KEGG" id="aho:Ahos_1226"/>
    <dbReference type="eggNOG" id="arCOG00676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067135_1_1_2"/>
    <dbReference type="OrthoDB" id="6768at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00975">
        <property type="entry name" value="Exosome_Csl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030850">
        <property type="entry name" value="Exosome_Csl4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034126">
        <property type="entry name" value="PRK09521.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0271">Exosome</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="S1 motif" evidence="3">
        <location>
            <begin position="66"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="20970" checksum="E13F6173A8E5AD6D" modified="2011-06-28" version="1">MKSQGEITFPGDLLGVIEEFSPGEGSYEEDGNVRSSTIGKVFYDMINRRSNVLSPKKSLMVTLRKAKYVYGIVNLIKEDVANVDIVSVEEKFITPISGLLHISQISNKRLNTILEAIRVGDVIKAKPLTFSPPISLTIKQKDLGVIFAQCSICGHMMTKLDEEHLKCVYCGNVEQRKVGNYVVRKYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S4</accession>
    <name>F4B3S4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription termination factor Tfs</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1228</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S4"/>
    <dbReference type="STRING" id="933801.Ahos_1228"/>
    <dbReference type="KEGG" id="aho:Ahos_1228"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="9">
        <location>
            <begin position="57"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="11643" checksum="8F14A3740CA17263" modified="2011-06-28" version="1">MMVPRKINGKNVYKCLKCGYEEEVKSSEVITTKIKHSETEKTLVLEEEMPQGAQKIKGVICPNCKNDEAYFWILQTRAADEPATRFYKCTKCGKVWREYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U2</accession>
    <name>F4B3U2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Conserved ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1246</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U2"/>
    <dbReference type="STRING" id="933801.Ahos_1246"/>
    <dbReference type="KEGG" id="aho:Ahos_1246"/>
    <dbReference type="eggNOG" id="arCOG01225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010340">
        <property type="entry name" value="PRK13768.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010342">
        <property type="entry name" value="PRK13768.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="28663" checksum="FBC6FAE3BC1A1511" modified="2011-06-28" version="1">MMYYIFFTGTAGSGKTTLVKEFQDYLLDQELDTAVINLDPAVEKLPYTPDFDVRDYVDAFEVMEKYGLGPNSSLIASIDLLMTKAVEIKNEVSEIEANYVLIDTPGQVELFAYRDTGRLISSLIVGDNKAANVFLMDSFLAREARTYVSLLLLSSAIRFRMNLPQVNVLSKIDLLTPKELEEIKSWSNGEELIDRLGEVDDYSFELVKTLIESLDSAPVPVSSTNDEGFDELYAELQRIFAGGEDYLTEENSPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V3</accession>
    <name>F4B3V3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extracellular solute-binding protein family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776056.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V3"/>
    <dbReference type="STRING" id="933801.Ahos_1257"/>
    <dbReference type="GeneID" id="10600738"/>
    <dbReference type="KEGG" id="aho:Ahos_1257"/>
    <dbReference type="eggNOG" id="arCOG00219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_793701_0_0_2"/>
    <dbReference type="OrthoDB" id="7820at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13540">
        <property type="entry name" value="PBP2_ModA_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050682">
        <property type="entry name" value="ModA/WtpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632">
        <property type="entry name" value="MOLYBDATE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632:SF16">
        <property type="entry name" value="MOLYBDATE_TUNGSTATE-BINDING PROTEIN WTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="344" mass="37501" checksum="C835C6712ED2F2E8" modified="2011-06-28" version="1">MNKLVIISIVIVAIIVIGTVAYLTTRTSTMMSKKTQVIIYVADAYTEEACCIGKCFSSATGIPVLSPESGGSFQLAREISSPNAQVTVFMPVALSAVNDLGNENPGWAIAFVSDQITIAYTNASISSNSCAQRALYYAKEAISTGNSSDWYEFYYILTSGKVKVGISNPNTDPAGFRAWINLEIAGYLFANNTYYFYDRMLNNHGNVTASNAAELVPELESGAIQFLYIYRSAAVAKGLDYIQLPAKLNLGCYKCSSFYVMFNYTLNTGVVKGSPVYLFITIPKNTNNYNEAIDFVIYVIEHSSILKKFDLCPLQPAILFNSTVVPPQIASLLTEGKLIEGGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B405</accession>
    <name>F4B405_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2299 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774886.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B405"/>
    <dbReference type="STRING" id="933801.Ahos_0076"/>
    <dbReference type="GeneID" id="10599516"/>
    <dbReference type="KEGG" id="aho:Ahos_0076"/>
    <dbReference type="eggNOG" id="arCOG01713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137738_0_0_2"/>
    <dbReference type="OrthoDB" id="33206at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd17510">
        <property type="entry name" value="T3SC_YbjN-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018747">
        <property type="entry name" value="DUF2299"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10061">
        <property type="entry name" value="DUF2299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17822" checksum="0C6176A49AEBBEAB" modified="2011-06-28" version="1">MDNEIKKWIQELGLMINVPPQAKEPFHVVMYPPQGGGPTLEVVRPPNQNLYIVIMGVGIHEIHQNALKEMKEDERRKFLNELKYDLLKMGVDIIFMPVGQEIPSAIQVSRVLLPDNLTANEFVNAVYIVRNAGLYIIFKFSDTFGTPQGKGGNIRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B413</accession>
    <name>F4B413_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774894.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B413"/>
    <dbReference type="STRING" id="933801.Ahos_0084"/>
    <dbReference type="GeneID" id="10599524"/>
    <dbReference type="KEGG" id="aho:Ahos_0084"/>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        </source>
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    <reference key="2">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32408" checksum="B247F7CA1759C161" modified="2011-06-28" version="1">MFVYYLIEAILSILGFYIGSELIVKGGKEIIHQGFMLGALSVLPELIVLSNLLFHKNFYLALSSFFITALTIYTLGVAVVNVSVFLKWRKRRISIVNFEERRIILIIMGILLSIFILQKINLEIGIISLFILSYYLILRLHEVKFSVRSLLFMLSGIMILWIASNNLVFMSSKFLPSWAFTIFLFPIVLNIQDIIVAIKGSLSSPELGPQMTLSFIVETIILSAFTLSLAGLLGGLKGVFIGINYSLLALILVSVTMFILFNNNNLCIKESIVLLSMYSLVPISILLHA</sequence>
</entry>
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        </source>
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="138" mass="15487" checksum="5857180856508CF6" modified="2011-06-28" version="1">MKKSDIVIIIFIAFVYILFFTNIITTASVEGVSMYPVFQNGALTFYGPPENIQYHNVIIYKSPEYGIYIIHRVVGIDGNHYITQGVDNISNPYPDNRIGLEPPQGIPSTDVIGKVTEVNGYIISIPYLGYLSILFSYL</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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            </authorList>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1342"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629676_0_0_2"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="AEE94225.1"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="72" mass="8240" checksum="B7EB3CE213EF38F6" modified="2011-06-28" version="1">MSVRIRGANAYGHLGWFQVYCRICNRKLTIGVDIVYKCPKCEKKYKAYFCEADKRGLKGKCPYCGSELVPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F1</accession>
    <name>F4B4F1_ACIHW</name>
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        <submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1036640_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="31373" checksum="5C83FE46C11E64DA" modified="2011-06-28" version="1">MARSFEALSKAYLASYGLLIVYPSLLIAAVKKGVYKAPRHLEKELNSLSILARQALNPKKMIEKLKHDPIGRSQLPDLLKSTSNFFQQNNEREFASLYNKISEFLRKPVNQRNYAEIMTLRKEITRVMQLKNVHERFYEILKKCIEGNINEDICKNLTQESKEMLSSFSNKEYLLDQILSILDLGFQEMFDGILYASYLARAAEIADYTVGRTEDDEKYLAEIIDHQQEIFDFLSKMQEINKVLIKDDELDDFMSQIEEEARKYFKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I0</accession>
    <name>F4B4I0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I0"/>
    <dbReference type="KEGG" id="aho:Ahos_0177"/>
    <dbReference type="HOGENOM" id="CLU_3263577_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="41" mass="4850" checksum="6F2D7B675222594C" modified="2011-06-28" version="1">MSTGATWSELLEKSRVSTETLLNFLDKLERLYIIEKVEKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N4</accession>
    <name>F4B4N4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="13">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_1381</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="13"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776179.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N4"/>
    <dbReference type="STRING" id="933801.Ahos_1381"/>
    <dbReference type="GeneID" id="10600869"/>
    <dbReference type="KEGG" id="aho:Ahos_1381"/>
    <dbReference type="eggNOG" id="arCOG04276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_2_3_2"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02888">
        <property type="entry name" value="RNR_II_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050862">
        <property type="entry name" value="RdRp_reductase_class-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013344">
        <property type="entry name" value="RNR_NrdJ/NrdZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02504">
        <property type="entry name" value="NrdJ_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371:SF1">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43371">
        <property type="entry name" value="VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="10 13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="14">
        <location>
            <begin position="17"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE94264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="841" mass="95795" checksum="23F4E833BC14558E" modified="2011-06-28" version="1">MIAMQTSTVSLLSLNKIKVIKRDGRKEEFKLEKILVKIGFVPSEVIDGIANDIIQNSKDNTIDTRTIADIVERNLIENSLEHPELMDLAKKYVLARIYNHVYGKGKWKDFDPKDLLLTYNALKVLEARYLLKDPNTLRYIETPQMMFRRVAKYLASVELKYGKSESEVKALEEKFYDIMSNLRFLPNTPTLMNSGTRLGILSACFVIPVRDSMTTPEGDGIYDALRAMALVHQQGGGTGFDFSELRPKGDVVASTAGVASGPVSFMKIFDVSTDVVKQGGKRRGANMGVMHVWHAEIEDFINSKSGKLKDVQLQNFNISVGVYDYFMEKVERGEEVPLITPRKTRIPGTDHDYYINKARNYMSEEWVQEEILNELEEKGGAVWLDESKIITVDEALVIAEKEGAIIRWINARSLFNEIVRSAWDSGDPGLLFIDEINRRHPVWYLGKINATNPCGEEPLLPWESCNLGSINLEKFVKETENGKYIDWDGLAETIKYAVRLLDNVIDANKYPLKQIEQATKSTRKVGLGVMGLARMLIKLGIPYDSVDAIYLSYQVAKFIYYHAFKTSIELAKEKGSFPVYDPLRYHDIWESARPFEYLLEVTKVEGKPSEYVRKLTAIVDKIDFSELKAERIKHGLRNATVVSVAPTGTISIIAGTSSSIEPLFALAFIRNVAVGKFMEIDSLFLEYLRKYELDNPEVVKKVAETGTVGENMFMPATIRKLFRTAHEVPPEYHLLHQAAWQQWNDSGTSKTINLRSEEPPETVERVYLMAWKLGVKGITVYRDKSKSQQVIYFGIKKEREEKEKREQEKKQQQIIPSSFRMEKKFVEVDENYAGGCKTCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P4</accession>
    <name>F4B4P4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="9 10">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">trm1</name>
        <name evidence="12" type="ordered locus">Ahos_1391</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="9 10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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            <dbReference type="Proteomes" id="UP000008458"/>
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    <sequence length="379" mass="42727" checksum="53660CDEF0670F31" modified="2011-06-28" version="1">MRLVEIKEGKARIFIPDPKEYEKNGKFDPSWAPVFYNPRMMFNRDISVIAVSIISPKSIIDALAASGIRGIRYYLESNSPIEEIIFNDKSKVAVDLISKNVEANGVKVAKITNRDANSLLYEVITDYVDIDPFGSPSPFILSSVNAVKNGGYIAYTATDLAPLEGSAKKSCKRKYDVNNSKLSFSKEVGLRVLLSKIVREAAVLEKAVEPIFAFYNDYYYRVIIRVTRGAKRADESIEKLGYFYECEKCGFSGSSKDPIEKCPRCGNKVKISGPVWLGKINDDSFVHKMIDYLQNFSYIKNFEAVSILLHKILQENKYPSGYYNLEFIASKYKINVPAREKIIECLGDASQTHFSSKGIKTSKSFDEILECMKTLSNKV</sequence>
</entry>
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            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
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            <strain evidence="1 2">W1</strain>
        </source>
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="EMBL" id="AEE93108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="23351" checksum="818B4B310EF86E64" modified="2011-06-28" version="1">MTKNEIHIAIDEAGNPNDEKPFIISAVLINDINCYLNLYEEAIKDAQRLTGKNIKYFKWSEDSPKQFKLGKDVKGIFVNKVLKNLTGISLIIKKVNETKLSNCSAKIYGEILGLHLRKYVASKSVVVHYDRTPILRESDKQYIIINFPKWTLAKVLRVDFTGHDKCELIHLADYISGIVREHVMKDELKEEYRQKVEDILML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
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        <name evidence="6" type="ordered locus">Ahos_0232</name>
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    <organism evidence="6 7">
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="AlphaFoldDB" id="F4B4V9"/>
    <dbReference type="STRING" id="933801.Ahos_0232"/>
    <dbReference type="GeneID" id="10599672"/>
    <dbReference type="KEGG" id="aho:Ahos_0232"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654888_0_0_2"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="2"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
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    <dbReference type="PANTHER" id="PTHR43687">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF1">
        <property type="entry name" value="FERREDOXIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="101"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="132"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="274"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="419" mass="47381" checksum="D7522FB5255FC30F" modified="2011-06-28" version="1">MKVKLYKLKDAEKFKEELIKSWPQFLPVPKVEYTDELICDGEPFIVVSNEFTKVEDCATLWAKPDPQDIIIKFIELNYALTEKGVNRRTFITGKQIEKFVRIPVINSNCLSSLGCNQCVTSCPQKAMSIVEGKVVIDENKCTYCGLCAASCPVGAINLSYPNWTTITILTKVIKKNKISISCDPKSADFLVPCIGVLGPEELYALHSFYDIELICPNEKCVNRQAALHAIDLYKEIKEKMKVKPSTFLSGNKRSDYVRIITAANISGESSFDIHGYKVNMNDNCTLCGACIRKCPTRSLKYNIKDNNVFIEFTPSKCVGCNKCVNVCEEDAITVNKSYNYSSLHEDTFYITAEDEVVRCKKCGRPFDNKKKILRVASLLNVDPTTLEYCNECKQTLVAEKILSNWKEKFGEIRRSKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X4</accession>
    <name>F4B4X4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775056.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4X4"/>
    <dbReference type="GeneID" id="10599687"/>
    <dbReference type="KEGG" id="aho:Ahos_0247"/>
    <dbReference type="HOGENOM" id="CLU_2339983_0_0_2"/>
    <dbReference type="OrthoDB" id="380764at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="97" mass="11222" checksum="62A1B558DD294CDE" modified="2011-06-28" version="1">MYNKKYILKAILGVEGILLFYSGIMLALTSNNEIKEMMFLAKVKPLIPIPFVFMIIAFIFNGTIFLLSISDRFMLFLIRNTFQSIIIRALMVFLSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B500</accession>
    <name>F4B500_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B500"/>
    <dbReference type="STRING" id="933801.Ahos_0274"/>
    <dbReference type="KEGG" id="aho:Ahos_0274"/>
    <dbReference type="eggNOG" id="arCOG08402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207589_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="52" mass="5577" checksum="C65124BC203941DD" modified="2011-06-28" version="1">MTGTAHEGGSDKSVDGRVVVFPSTSPKDLRVTVYDPLRGVPVAELEDKLRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B539</accession>
    <name>F4B539_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleotidyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1458</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776256.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B539"/>
    <dbReference type="STRING" id="933801.Ahos_1458"/>
    <dbReference type="GeneID" id="10600954"/>
    <dbReference type="KEGG" id="aho:Ahos_1458"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="4">
        <location>
            <begin position="249"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="355" mass="39037" checksum="9EC501938CC2DC79" modified="2011-06-28" version="1">MKAVVLAAGKGEGLRPYTEKEQKEAITILGKAVISHVLYGLKKAGIEEVVIVTNEHEKQIQDAINVDIPFETVRQKRPGITGAVLDGMDKIPDDEFLLAFGDIIAEPEFYMNLMNSYITGNSKAVFSLVPVSEGMQTYGLAKIVDNRIEIVNEGSTLALAGAYIIPKGDFTDILEYFKKISPFSRYFIWSGKWIDIGYPEDIINAIEVLLSDLNNSIISDKAEISKTAVIGKKVIIEDNAIVDDYSVVKGPAYIGKNAYIGNYSLIRDYSSIESEAKIGAYCEVAHSLVEPRAEIGSKSYLTYTIVGREAKIGASVITVSYPANPVRGRERKLGALISPNEEIYHGQIIGPNYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B556</accession>
    <name>F4B556_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776273.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B556"/>
    <dbReference type="STRING" id="933801.Ahos_1475"/>
    <dbReference type="GeneID" id="10600974"/>
    <dbReference type="KEGG" id="aho:Ahos_1475"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="15264" checksum="3DCF32335B190E89" modified="2011-06-28" version="1">MFEKILVAYDGSEHSKKALDVAIDLAKRYSSEVYVVEAVDETIFETVGVLPPLSAIEEMEKKAKNDIDEAVKKATQNGVKAVGEVLSGDPATAILEYANKNDIKLIIMGSRGLSRFKRILLGSVSSRVVQESKIPVIVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B578</accession>
    <name>F4B578_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B578"/>
    <dbReference type="STRING" id="933801.Ahos_0281"/>
    <dbReference type="KEGG" id="aho:Ahos_0281"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14559" checksum="033E7CF02B58C574" modified="2011-06-28" version="1">MMQILKTSADVYLEEADELLDRGDTLQASEKYYKAAEEAIKILSGILLEDDIISEVRNKDWNTEIINKAVFKLSSILGKWIIESWGSAIALITVNLDAQQIKKYREGIVRLVQVADERFNRKIIERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B586</accession>
    <name>F4B586_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sugar ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0289</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775096.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B586"/>
    <dbReference type="STRING" id="933801.Ahos_0289"/>
    <dbReference type="GeneID" id="10599729"/>
    <dbReference type="KEGG" id="aho:Ahos_0289"/>
    <dbReference type="eggNOG" id="arCOG00160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050901">
        <property type="entry name" value="BP-dep_ABC_trans_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243">
        <property type="entry name" value="MALTOSE TRANSPORT SYSTEM PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN TRANSPORT SYSTEM PERMEASE PROTEIN MALG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="58"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="29555" checksum="285F0CC1E3BF19C0" modified="2011-06-28" version="1">MVLFSVFPLYYIIMTSFSNAPNLISLDVSDLFPKHIYFTAYRELLTTNYYGGSFPLWIRNSLILASSTALISILLAMLTGYALSRLNIPAKKTLAAFIYIVTFFPYTATAVPLYLMFAKFHMLNYIGLIIAYTPGTSIFAAFLAKLAIDSIPPSYEEIAMVDGLSRFQVFLRIVMRLALPIVALTAILSFSGAYLDFALAYAFLLPHISDWTATIGLYYAAGLLNYASAPAYNLFAAGSVIMGIPLTALFIASQRMMTRAYSTLAGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B590</accession>
    <name>F4B590_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775100.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B590"/>
    <dbReference type="STRING" id="933801.Ahos_0293"/>
    <dbReference type="GeneID" id="10599733"/>
    <dbReference type="KEGG" id="aho:Ahos_0293"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="299"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="383" mass="45260" checksum="098BAED4D42A8EEF" modified="2011-06-28" version="1">MARRGNKAIRATVSMKIALSEPLLVLVNNYVKALRFTLFWLKENVPNPNEDGVISKVHEALYEKLREEYNLPSKVAEDCYRDALSIYKGWYNNPKKGRFPRVYKPTAWLTPKQSYNVDFEAMTVRIASVDELPILGYPRNLKEYMSWRMREARLTVKDGKAFLKVVFEKPREENVELKESIAVDINMAEVVVGKDDTHYVRIPTRLDEVHHWKSLAENLQKKYPRRWRENKRVLHRIYSFHQKARRVMEDFARKVGKWVVEIAEDFGANVIKLESLKNLIKNVDKLPREFRDKLYLMQYRRLQYWISWQARKHGMIVKFVNPSYSSVSCPKCGRKMREVSHRWFRCSCGYENDREVIAIVNLNGRGSLTLSSAPQMRDINPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A2</accession>
    <name>F4B5A2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="242"/>
            <end position="264"/>
        </location>
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        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
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            <begin position="323"/>
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            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="440" mass="49085" checksum="FB8EC9ED98DCB789" modified="2011-06-28" version="1">MKGFIYEVKKGILNKTFITFLLIFTLLSVTVSLHTVQKYQPESNLNFIASITPLYNGKFLIEGFVSFKDVPLYKAEIMVGNTTLLTNKQDFFVGLINNSGKIEVKYESLNRCFNFSPVIISQACAYTYSIDSPNNKIIFEIINYTQQTGRAVIIVITPQAGVVYYNVSKPNATVNFGASVCAHIPKHLTFKKLGYVNFTAEFPIYLHRGVSYITLKECNMTSIGTFLSTSLYESHIICTSSAALGLFAAFFPIIISYEASTLFARELDMGLLTYLLSKPISRVKLFLRRVGVGFISSVISSSVIFLVIEAILSTYNAFYFPLLYFWLGETLALAGYFMLIIAISVFVRKSNIVSGISVFVYIISLFMLFIASPVFFNGSLQAQEILSYLNPLGATVLATYQIPLDAAYGTVPLSFFLLDAIGWIAVLLVLSLLRFKKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E6</accession>
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        <lineage>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0349"/>
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    <dbReference type="Proteomes" id="UP000008458">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050276">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43617">
        <property type="entry name" value="L-AMINO ACID N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
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    </dbReference>
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        <location>
            <begin position="21"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="20457" checksum="AF250A03A2B2A37A" modified="2011-06-28" version="1">MTRNKDSNMYLNFHSKVLCMKEIVMKKADDWRKVYELYSRLSNEDLYLRFFHYYRPSEEEVRKLVTEQEDHITIIAEDEEGKVIAEGTIYDDGEFSLVVDPNFRMRGIGTAMVEKLIELGKEMGLKKIRFYTLPENIPMIKIGKKLGFKLTFTSDEVYGEKPLILERVTSYVSS</sequence>
</entry>
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    <accession>F4B5I9</accession>
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        <submittedName>
            <fullName evidence="2">DNA polymerase beta subunit</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1530</name>
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    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I9"/>
    <dbReference type="STRING" id="933801.Ahos_1530"/>
    <dbReference type="GeneID" id="67200063"/>
    <dbReference type="KEGG" id="aho:Ahos_1530"/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NT_sf"/>
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    </dbReference>
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        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="18"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="65" mass="7495" checksum="5CC06D92FEE04194" modified="2011-06-28" version="1">MEEDIIKRRIKERERVINEAKNFANSLKGSFSAFLIGSYARGDFNAWSDVDVLIIGNFMEENPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P2</accession>
    <name>F4B5P2_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">CRISPR-associated protein, Csm2</fullName>
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    <gene>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P2"/>
    <dbReference type="STRING" id="933801.Ahos_0366"/>
    <dbReference type="KEGG" id="aho:Ahos_0366"/>
    <dbReference type="HOGENOM" id="CLU_2055925_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="119" mass="13552" checksum="EE89EA5D6247FE2F" modified="2011-06-28" version="1">MDYKRKGVEDICKIKKDFAYSNNQDGKLTKSLIRKIFDMINDSQNLSSIIPDLAYLAARNKGLSNDTELGRFIISLLNLIRQQPRDNVVKYVEGAVMAVYIIEEAQSNDLDPFKFLDCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R3</accession>
    <name>F4B5R3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase M20</fullName>
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    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0389</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775194.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R3"/>
    <dbReference type="STRING" id="933801.Ahos_0389"/>
    <dbReference type="GeneID" id="10599829"/>
    <dbReference type="KEGG" id="aho:Ahos_0389"/>
    <dbReference type="eggNOG" id="arCOG01110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029469_2_1_2"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005034">
        <property type="entry name" value="PRK06446.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="4">
        <location>
            <begin position="181"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="437" mass="48939" checksum="DFF35B6F2AF2C5DA" modified="2011-06-28" version="1">MEFSKEELDFFLSKYQEFLKIPSISATGEGIRNASSWLKEFMEELGIKTEIVETKGHPVVYGKVNNGGSKTLLVYNHYDVQPVDPINEWKYPPFSATISDGYIFARGASDNKGTLIARLLAFSKYKGKLNFNFVFEGEEEIGSIHLNEFIQSRKEELSKSSAVIMEGAGLDTKGRPMIVLGVKGLVYVQITVRVGERDVHSSVAPLIKNPVWELIKILNTIYDGEKVKIEGFYEDIEPLSKEVEELLDKIELDVEEYRKSLGVYSFNYKDRKEVVKALYTYPSCNIDGIFAGYIGQGSKTIVPSYAMAKMDFRLVPKQDPEKIYRELEKIVKAMGGEIVEMGLEKPVRTSPNTAVVKAMISSAVKAYSKDPVILPNAAGTQPMGLFYDLGIKEIVSAIGVGTPSSNAHAPNENVKLENFYKAIEHSLYFYQEYESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S1</accession>
    <name>F4B5S1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775202.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S1"/>
    <dbReference type="STRING" id="933801.Ahos_0398"/>
    <dbReference type="GeneID" id="10599838"/>
    <dbReference type="KEGG" id="aho:Ahos_0398"/>
    <dbReference type="eggNOG" id="arCOG00131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051127_0_0_2"/>
    <dbReference type="OrthoDB" id="9393at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="232"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="416" mass="46179" checksum="62C7CE4773884F98" modified="2011-06-28" version="1">MIKYSTKWMYLVIPFNMAIGPISTLVTLQILTLGGNAIDVAYVISLGNAVLIPASIMWGFLADRMDRKKQILLSFAGTALPLLLMPFFNSISFVAINYSLLVFMSAASTTPFNLLVMESAEKKHWGSLFSKFSFFSSIGMLLGLLASTFLVIFLKIYEVEEILGILTLGTLIAGFKLVPRPVITFERTAMLQHKESFMIRIRHLPLFFLHLPNIHSFKVFSLSRLTKKPINYIPLLYLGLVIFYISSGIFNTVYPAGLYVKGLEKSEVLLVITVGMVFQILGFRISSSLLEAKEESKIAHLSLILRGSSYVVLGAFSVFFLGYPILISGLIFYPLAAGIAYSLFYSSSTTMIFKIVGERQQGRGLGVYSTVVGISLFLGSLVSGYITHYLGYGIDFIIAGLLLLADSYLFRYLEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Y9</accession>
    <name>F4B5Y9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776402.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Y9"/>
    <dbReference type="STRING" id="933801.Ahos_1609"/>
    <dbReference type="GeneID" id="10601109"/>
    <dbReference type="KEGG" id="aho:Ahos_1609"/>
    <dbReference type="eggNOG" id="arCOG00894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1068007_0_0_2"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="29344" checksum="E01B34DB33FAD597" modified="2011-06-28" version="1">MKISVIIPTLNSERTIKLAVSSSLKVADEVIVVDSFSTDRTVEIAEKEGARVLQVRGSRLIARIEGAKVARGDYVVNLDSDMYFSENFKGFEEKVIALGEITVGRGIVHKLMSVDREITHKKWAENLSVCNGGIIPRMYDRQLLLKAYEMIPLNLKDRLNAFEDSVIYYNVMKLYKEGKVQFIPNAVYHIEDDSLWQFMRKWYKYGKNAKLLRGTEYERFIYERKSRPGLTLGEKMKLLPITLIKGIPFALGYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Z1</accession>
    <name>F4B5Z1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776404.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Z1"/>
    <dbReference type="GeneID" id="10601111"/>
    <dbReference type="KEGG" id="aho:Ahos_1611"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <begin position="1"/>
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    </evidence>
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    </evidence>
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</entry>
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    <accession>F4B625</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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    </evidence>
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    </evidence>
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    <sequence length="81" mass="9455" checksum="CEED1566C9571D20" modified="2011-06-28" version="1">MIEYPPILDLKEKLIIIYPTRSDYELAIKIMVKLRKIGEPVNVVDIILSSIAINRDMIIVTNDNDFESIKKVEERLKIEKL</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </evidence>
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    </evidence>
    <sequence length="68" mass="8249" checksum="C44D9810734AAF93" modified="2011-06-28" version="1">MVLWKYKDKVSEFTLVISDRFTVNAEIPFDKIERVDNYYIYLTDGETVIPIHRVLEIRRNGRTIWSRK</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
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    </dbReference>
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        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049:SF16">
        <property type="entry name" value="PROTEIN VDLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="153"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="34662" checksum="4E0D598AC2800E60" modified="2011-06-28" version="1">MKLSDTKVRTQNIVHYEQTNFMGRLHGGDMLKFLVDTGMLSAIKVAKATSVIASLDNVVFKKGANLGDIIEVEAYVAYVGNTSMEVEMSAYRGDEKIVTATGVYVKIDENFRPQKVGEIIECDNEEEKAIIERAKKRRQNRIRSIKNNEDQTKGLRFRITNGVYISPDLTYDGKIISAGKLLKMMDDLGGSLALKFIGYKGYDKYSDTVVTVAISNMSFYSPIKLGDIIEINAGLTYVGRTSLDVLINVFKNGKEKVTEAYFSYVRVDNEGKPKEMPRYYPETEVEKNLWQEAIKRRNLQIKEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B8</accession>
    <name>F4B6B8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tatC</name>
        <name evidence="6" type="ordered locus">Ahos_1660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776453.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B8"/>
    <dbReference type="STRING" id="933801.Ahos_1660"/>
    <dbReference type="GeneID" id="10601160"/>
    <dbReference type="KEGG" id="aho:Ahos_1660"/>
    <dbReference type="eggNOG" id="arCOG01919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_3_2_2"/>
    <dbReference type="OrthoDB" id="15305at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="275" mass="31265" checksum="8910F02BB6D042ED" modified="2011-06-28" version="1">MTERTELQKDEERPLLSHLAELAIRLRRAFITLFVAFIIFFAFGYTTISVSGHVVPIIYPNLFHSFSDELMLFFIHHELPPQLKLLNLNPFDPLYASAYVSFYMSIFISLPIFLKEFWGFVSPGLYEHEKRLIKYTITPAILLFAAGSLFAYFIIVPLMMKFVVLYTTALGVEPTLSLRAFVSTVVSLMMVTGIAFEYPLVMASLTYIKVVKADSWKKNWRWGVMGAFIIAWIISPGTTGGVIETTIGVILSVLYFVGVVSAKIIEKRSFRNTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B9</accession>
    <name>F4B6B9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbonic anhydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776454.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B9"/>
    <dbReference type="STRING" id="933801.Ahos_1661"/>
    <dbReference type="GeneID" id="10601161"/>
    <dbReference type="KEGG" id="aho:Ahos_1661"/>
    <dbReference type="eggNOG" id="arCOG02860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118421_0_0_2"/>
    <dbReference type="OrthoDB" id="24878at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="185" mass="21153" checksum="460AB8FC89B45641" modified="2011-06-28" version="1">MVRLIISCMDYRLSQEVMNRVKDENDIIIRNAGANIYELKDRLKGINADEVVFLPHTDCAAMKLVNSAIKDGKDVDKEIDEKLVAQFRGKEFSSLQELEKLNAEIGEKMLKEIFPNAKITTELIDVNKIKIPQKKTRYYLLKPQTRYNDEIIGSYVIQAFDKEDVTADIKIAESLGLKLEKSELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F4</accession>
    <name>F4B6F4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SirA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776489.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F4"/>
    <dbReference type="STRING" id="933801.Ahos_1697"/>
    <dbReference type="GeneID" id="10601197"/>
    <dbReference type="KEGG" id="aho:Ahos_1697"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_1_2_2"/>
    <dbReference type="OrthoDB" id="45650at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF6">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="11"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="80" mass="8865" checksum="784CFD8255E39A93" modified="2011-06-28" version="1">MSQEVKIAKTLDLKGMFCPGPVLETAKAIKTINVGEVLEVYATDPAAKSDLEAWARRTGHQIIDMKQENGVLRVLIKRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I6</accession>
    <name>F4B6I6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776521.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6I6"/>
    <dbReference type="STRING" id="933801.Ahos_1729"/>
    <dbReference type="GeneID" id="10601229"/>
    <dbReference type="KEGG" id="aho:Ahos_1729"/>
    <dbReference type="eggNOG" id="arCOG00726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911278_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="10"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="61" mass="6963" checksum="AEF6E01A22184773" modified="2011-06-28" version="1">MLVVNQGEYLIEAFKIANRNKITIYDSLFIALAKSMNLELVTSDKRQYEIAKNEGVNTQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K5</accession>
    <name>F4B6K5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CDP-archaeol synthase</fullName>
            <ecNumber evidence="11">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">carS</name>
        <name evidence="12" type="ordered locus">Ahos_0527</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775331.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K5"/>
    <dbReference type="STRING" id="933801.Ahos_0527"/>
    <dbReference type="GeneID" id="10599968"/>
    <dbReference type="KEGG" id="aho:Ahos_0527"/>
    <dbReference type="eggNOG" id="arCOG04106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="OrthoDB" id="45383at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003114">
        <property type="entry name" value="PRK04032.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650:SF1">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="164" mass="18280" checksum="343831862C1CACCB" modified="2011-06-28" version="1">MLEELLIALVIYFPAFTANGSAPFVGRGHPIDFNKNFFDGRRIFGDGKTFEGLLVSLTFGTIIGVIIARFWGVSWIFISFVESLAAMLGDMTGAFIKRRLNIPRGGRALGLDQLDFVLGSSLALVLLHVNIELCQFLFIILIAFIMHILTNNVAYRLKIKSVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L7</accession>
    <name>F4B6L7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L7"/>
    <dbReference type="STRING" id="933801.Ahos_0539"/>
    <dbReference type="KEGG" id="aho:Ahos_0539"/>
    <dbReference type="eggNOG" id="arCOG08310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021541_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="126" mass="14380" checksum="42394EBE4B98C43A" modified="2011-06-28" version="1">MLDMRPLVAIDLNSNIDYLVLFKFINNLLRKFKDVDITFIVDDGKILEFGNDEVFKISDSYSTVELVRDLKSISDKGDSLKLGSLLKLKRELGRSIVILVSDRKVKSKGELIFVFDGKRIRLLNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L8</accession>
    <name>F4B6L8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775344.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L8"/>
    <dbReference type="STRING" id="933801.Ahos_0540"/>
    <dbReference type="GeneID" id="10599982"/>
    <dbReference type="KEGG" id="aho:Ahos_0540"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="141" mass="15052" checksum="67281C198B7C7B9E" modified="2011-06-28" version="1">MFKHVLVAYDGSSHAKRALEIAIDLAKKYEAKLDIVEVVDSTVFAGAGIAPVPADVIESVYNRAKADIEEAKKKSKEGGVEAEGVILEGDPASAILEYANKNNVDLIVTGSRGLSTLKRVFLGSVSTRLVQEAKIPVMVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N2</accession>
    <name>F4B6N2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6N2"/>
    <dbReference type="STRING" id="933801.Ahos_0554"/>
    <dbReference type="KEGG" id="aho:Ahos_0554"/>
    <dbReference type="eggNOG" id="arCOG00216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF19">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN MJ0666-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="173"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="383" mass="42605" checksum="CE20ECF23C84738B" modified="2011-06-28" version="1">MLIPLEEARKIIEETEFPKPSIREVDLLDSVGKIVAEDILSIEDIPEKDLSAMDGYAIRTEDAGKKLKVVGSLFPSSKEIPRLNEGEAFYVTTGAPIPEGADAVCRIEATKREGDYVIIGEKVHKGKDIRPKGEDIKRGEKILNKGEKISPYHLGILAYEKIYKIKVLNVSFAVFANGDELSPFHSNGEKVDSITPILIPLLSSFGSVTYLGVAKDNPEDVEKFIEKASSFDFIISIGGSSVGEKDYVKRVIEKKGGKLLFEGVSVNVLKRGSVGLINGKPILILPGQVVSAITVFHEHGLHVISRMIGKEIRKFEEIELGEEIEIRHNMDSTFLFENKGGKAYPLRWGVGLYSELYKASWFGVLKRGRKYERGEKVLLQRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Q2</accession>
    <name>F4B6Q2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0574</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Q2"/>
    <dbReference type="STRING" id="933801.Ahos_0574"/>
    <dbReference type="KEGG" id="aho:Ahos_0574"/>
    <dbReference type="eggNOG" id="arCOG01391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041759_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050321">
        <property type="entry name" value="Glycosyltr_2/OpgH_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867:SF2">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="8">
        <location>
            <begin position="20"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="431" mass="48778" checksum="2CC3C36CE95C1AFF" modified="2011-06-28" version="1">MGYNRIDAINVNPKDLPLLSIIVPTKGERPELIQGLLDNLAEVDWNKSRMEVIIVSDDSPEYFEEMKEKIVPPQGLEVRMYRREKKLGFKSGALAYGFEKSRGDLILTLDVDARVKKDSIKKAYFHMLQFGCDAVTMKWIGYSHNGYSLLAKGIMVSTIIADNSILRGRDRANLKVFPVGCGTMFKKSAIEEVGPWDYNMIQDDLEIGARLINHGKRICSSDSIVFVEVPDNLLAFYIQQTRWAMGSIEVLTRRFKQIITSKANILQKIDALLFLLQYVPIALTFIAALLISVYSFLDKGDPLNSIPLFTLWAIALGIYAYNFIKVARDSGIKLIEALRSLGKISAYTVSISPFITLSIFSALKKSRNYIITPKGKAGKTKIVYIILAFGLLFLFASINYLLHQEFISGLWLLYYSLAYIFTFSSYKQEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Q7</accession>
    <name>F4B6Q7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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    </reference>
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    <sequence length="39" mass="4523" checksum="20291E7041706513" modified="2011-06-28" version="1">MIVRRIKGMTIIFPSEEAMKEVLDDTEIEDIIDVNKESN</sequence>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <location>
            <begin position="156"/>
            <end position="177"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE94656.1"/>
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    </evidence>
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        <source>
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    <sequence length="182" mass="20924" checksum="1875F11174C5CB5B" modified="2011-06-28" version="1">MGMVLSYNMFYPTTRTISDYAQQFTFNFILSVITLILVVLVIKNIAFGTGQDFFDGTIITFLQMKGRKKVFLSLYVTDVIIPGVIFILTTMTTFYLASFNVSLTWIPEYLSIYLFLSSASYIVTILTKRPFRAFFISIVILLLFVGVDFSQNFMLIYCYLTMMILSIILLIASYYLFRGVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W5</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11 12">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
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    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
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    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776583.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X5"/>
    <dbReference type="STRING" id="933801.Ahos_1792"/>
    <dbReference type="GeneID" id="10601292"/>
    <dbReference type="KEGG" id="aho:Ahos_1792"/>
    <dbReference type="eggNOG" id="arCOG00039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081854_1_0_2"/>
    <dbReference type="OrthoDB" id="6532at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="22429" checksum="5AF4E03980D68ED9" modified="2011-06-28" version="1">MKLLLYSGGINSTVALYRFKDIVDCLFIDYGQRSARMQREMAKYHCEKLGKKLIEIHIPSLGKAFYDGLGMRLNEPIVHRNVILLSIALTYAKERKYDEVIFATASEECLFEKDKPLIISTMKRLAEIYGVKLSMPFVNLSKSLLVKMGVKQGIELEKTYSCLLGHKYHCGVCSQCQTRKIAFKEAGVRDETVYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B706</accession>
    <name>F4B706_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B706"/>
    <dbReference type="STRING" id="933801.Ahos_0601"/>
    <dbReference type="KEGG" id="aho:Ahos_0601"/>
    <dbReference type="eggNOG" id="arCOG01679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156332_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="112" mass="12505" checksum="648D9F0301E2BE41" modified="2011-06-28" version="1">MNKVFVSFLFMELVVTDPEDILRVSTAFSSMTRINILKLVAEKPKSITELSNELMMSKGNISSQVAELQNAGLIEISYENGEKGIKKMIKSKYDRIIIVLSESGSSLQESNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B748</accession>
    <name>F4B748_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775447.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B748"/>
    <dbReference type="STRING" id="933801.Ahos_0644"/>
    <dbReference type="GeneID" id="10600093"/>
    <dbReference type="KEGG" id="aho:Ahos_0644"/>
    <dbReference type="eggNOG" id="arCOG01831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914055_0_0_2"/>
    <dbReference type="OrthoDB" id="18771at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="122"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="302" mass="35046" checksum="EF87562DBBC4CAEE" modified="2011-06-28" version="1">MGRDYFLDKDIIKDKNDNLYVVITNYNPPGYVFAYLKYVYTGEGLWKGYERVFKKYGVKNLIKIKQNFEYEPCYGSTFPVLKISEIKNHFKPEDKIKEIIHKPANKLEETVINMLAEMEVNLPLDKIGVTGSLLAGISHENSDVDLVIYGKKYAEDFVNTFRGFEEDKDWIIETSENYSLPIEVVKTIYSKKTRGKYKGVKYSFLFVDDHPWKYNEKVCIEVNPIKAIGENVSDYRALFYPSISTLYSQGKTFNIVSYEGIYSLALYYSKKIEAFGMLMKCEDELEIVIGDKKIGGYIRPIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B774</accession>
    <name>F4B774_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IS607 family transposase TnpA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776609.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B774"/>
    <dbReference type="STRING" id="933801.Ahos_1819"/>
    <dbReference type="GeneID" id="10601319"/>
    <dbReference type="KEGG" id="aho:Ahos_1819"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="OrthoDB" id="28414at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="2">
        <location>
            <begin position="55"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="189" mass="22098" checksum="63D30EB94270B6AE" modified="2011-06-28" version="1">MLKPKEVCQKLGISYRTLQSYVKKGYIKPVILQSGKWRFREEDVERLMGIVRKRKVVLYARVSSQKDDLINQVKYLEENVKDYDQVITDVGSSLNMKRKGFLKLLRMILNNEVSKVIIAYPDRLVRFGFEIIEEVCKAHNCELVVLNNEDKTPEKELIEDLISILVSFSEKLQGMRKEKVKKCVEELKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A2</accession>
    <name>F4B7A2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1849</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776637.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A2"/>
    <dbReference type="GeneID" id="10601348"/>
    <dbReference type="KEGG" id="aho:Ahos_1849"/>
    <dbReference type="eggNOG" id="arCOG09707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392716_0_0_2"/>
    <dbReference type="OrthoDB" id="33483at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="10922" checksum="BAAB9A9A33B42085" modified="2011-06-28" version="1">MILKPKISWFCLNRLKTPYVYWKSVIVILENPSKTLVVDVSEDQLSTYRPPKEIINFKFFYKIGKIDDENIKYIGCIAQELQKNMKAIPQPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A8</accession>
    <name>F4B7A8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aldehyde oxidase and xanthine dehydrogenase molybdopterin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1855</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776643.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A8"/>
    <dbReference type="STRING" id="933801.Ahos_1855"/>
    <dbReference type="GeneID" id="10601354"/>
    <dbReference type="KEGG" id="aho:Ahos_1855"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
    <dbReference type="OrthoDB" id="57164at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="11"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="684" mass="75189" checksum="2F85C82AC00A2BBB" modified="2011-06-28" version="1">MRRLDLEDVIKGKGNYVDDIPFSGYYGVFVRSQYPHAILRKVDVDGVRRKGALVLTAEDLLSLPNLKGKEEQEGSSSISSLLAFKKVRYVGEPIALVIAKDPYEATDLAEEVSVDYEPLPPVSNIDEALKNDVLVFDEVGTNEVYKKTFEYGEMPKGDEELEVSLYWSRSSGNPIETFGVISIPQGDEVTIISNVQAPQFQSNEISKYFKVKVVPTRHGGSFGSKFSVTRYAIVLGLASLKFNIPIKWIETRSEHLMASNSSGPERKFRIKAYFKRDGTVTGLDFKIWEDIGASLYNGQAFKPTGILAGPYKVKNIRYDVSLVATNKNPPGAFRGAGTPPHTWALERVMDFIADELKMERHEVRRKNLIDSFPYDAPYAHYDSGNPRNLLELALSRKDLWSLREKGYGVGLACSTDPSTPSGQEGVKLSIKKGKVILGIGYGPEGQGNEHVGKLLVSRLLGIPPEAVEVELLDSNESPSSFGPGGSRMAVFLAGAIKGAVEELVSKLKRKYNAEYKDGFLIRGEEKIPITSLQEEVSYVYSQQGKTRFIAYPFACDIAVVKVDEETGLIKPVKLVVYLDPGTPLDEEVVKEQVIGGSAIGVSVALYESYVYENGQLLTLSLSDYGLPTADQMPEFEINLVPTPSPYTPFGEKGIGEIPVGVAAAAVTSAVEDAIKRRITKVPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F7</accession>
    <name>F4B7F7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">protein adenylyltransferase</fullName>
            <ecNumber evidence="9">2.7.7.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_0675</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-tyrosyl-[protein] + ATP = O-(5'-adenylyl)-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-(5'-adenylyl)-L-tyrosyl-[protein] + ATP = O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.108" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775478.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F7"/>
    <dbReference type="STRING" id="933801.Ahos_0675"/>
    <dbReference type="GeneID" id="10600126"/>
    <dbReference type="KEGG" id="aho:Ahos_0675"/>
    <dbReference type="eggNOG" id="arCOG04066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217517_0_0_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009185">
        <property type="entry name" value="Nucleotidl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052038">
        <property type="entry name" value="Type-VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33571:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MJ0435-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33571">
        <property type="entry name" value="SSL8005 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotidltrnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="13">
        <location>
            <begin position="18"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="220" mass="25754" checksum="24578B50295199FF" modified="2011-06-28" version="1">MQIEYTEEQWKLFNEKRRRAKEILESLYYRGIKGYAYGSIARGDVKKTSDIDIIVFEPNILELDLLEADHKYIIQATPISTPKAYISLNPEETEVISFPLSKLKKDEEEFYYFGGLVSLEDIINGVRKPGINKELKLIIPNKNGHEEIPLKGNEDYATRLLKISITTINEREKLLMKREDKGRTGVFLKYELATNENFEEAIRELSRRNKFFRRALNDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J8</accession>
    <name>F4B7J8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonuclease H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775519.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J8"/>
    <dbReference type="STRING" id="933801.Ahos_0716"/>
    <dbReference type="GeneID" id="10600170"/>
    <dbReference type="KEGG" id="aho:Ahos_0716"/>
    <dbReference type="eggNOG" id="arCOG02942">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095977_0_0_2"/>
    <dbReference type="OrthoDB" id="52651at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09279">
        <property type="entry name" value="RNase_HI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053576">
        <property type="entry name" value="RNase_HI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041175">
        <property type="entry name" value="RNAseHI_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387:SF2">
        <property type="entry name" value="RIBONUCLEASE HI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="148" mass="17050" checksum="4FD5E964DCD8AAAA" modified="2011-06-28" version="1">MAKVNFDGLCEPKNPGGIATYAYVIKIDNQVLQGYGLAEKPWSKDSTNNVAEYMGAYCAIRKLIQLGITKAIFYGDSQLVIRQLSGEYRIKSPRLKRIYDKIQESLKNFEHIEFKWIPREENTEADRLTRIAYKLALENKLKEIGCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N4</accession>
    <name>F4B7N4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776696.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7N4"/>
    <dbReference type="STRING" id="933801.Ahos_1908"/>
    <dbReference type="GeneID" id="10601407"/>
    <dbReference type="KEGG" id="aho:Ahos_1908"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="299"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="383" mass="45362" checksum="918782E97ED0245B" modified="2011-06-28" version="1">MARRGKKAIRATVSMKIALSEPLLVLVNNYVKALRFTLFWLKENVPNPNEKRVLPKVHEELYTRLRMEYNLPSKVAEDCYRDALSIYKSWYNNPKKGRFPRVYKPTVWLTPKASYNVDFDKMTIRIASVGELPILGYPRNLKDYLSWRMKESRLVIKGDKAFLKVIFEKPREESVEPKESVAVDINMAEVVVGKDDINYVRIPTRLDEVHHWKSLAENLQKKYPRRWRENKRILHRIHSFHQRARRVMEDFARKVGKWVVEIAEDFGANVIKLENLRNLIKNVNKLPKEFHDKLYLMQYRRLQYWISWQARKHGIIIKFVNPSYSSVSCPKCGKRMEEKGYRWFKCSCGYENDRDVIAIVNLNGRGSLTLSTAPQMRDVRANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P5</accession>
    <name>F4B7P5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776707.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P5"/>
    <dbReference type="STRING" id="933801.Ahos_1919"/>
    <dbReference type="GeneID" id="10601418"/>
    <dbReference type="KEGG" id="aho:Ahos_1919"/>
    <dbReference type="eggNOG" id="arCOG05710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117974_0_0_2"/>
    <dbReference type="OrthoDB" id="10147at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="158" mass="17823" checksum="33C54F233F322D25" modified="2011-06-28" version="1">MPKELTPLEKLQLLVPGYRGYKVKDLIRQDDFLVRQSTISKLENAITNISQQEAQIAAIEPFSPALKKMEYIVSQLRTIIGIINSQQGGGADVYARYKIQTEQLDEIVNNDLQMVSIANEILNLSSSPDTVDAILSMVSKLKEVVINRNKLFYPQEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P8</accession>
    <name>F4B7P8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="119" mass="13439" checksum="83319473F4865C2C" modified="2011-06-28" version="1">MLSLKTSADIYLEEADELLKKGDTVQAAEKLYKAAEEAIRLLSFILKLNLEIVNTKSLTDAVFLISERLKDDKIPIYWASAISLITVNLSKEVVKDLRNDVEELVKIADREYIKVLGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P9</accession>
    <name>F4B7P9_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1923</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P9"/>
    <dbReference type="STRING" id="933801.Ahos_1923"/>
    <dbReference type="KEGG" id="aho:Ahos_1923"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="112" mass="13203" checksum="282DDE319F6B08EE" modified="2011-06-28" version="1">MEEADELLKKGDTVQAAEKLYKAAEEAIKFLSYRENIGILSKVKKEDRWKSEFLFDASLQLSNSYPEIKDIWKSAWILHVEGFHETRLDENKVKFYAENVKKLRKILEPLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R0</accession>
    <name>F4B7R0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome bd ubiquinol oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1934</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R0"/>
    <dbReference type="STRING" id="933801.Ahos_1934"/>
    <dbReference type="KEGG" id="aho:Ahos_1934"/>
    <dbReference type="eggNOG" id="arCOG02721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030555_3_2_2"/>
    <dbReference type="OrthoDB" id="41618at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365:SF14">
        <property type="entry name" value="CYTOCHROME BD MENAQUINOL OXIDASE SUBUNIT I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="CYTOCHROME D UBIQUINOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="468" mass="51215" checksum="FBA4D9663B060A12" modified="2011-06-28" version="1">MATHVLFTYWAIALPFFIISAEYLAYKKNDPYYLALAKRWSVVMAVLFAVGSAAGAAIAVEFITVWYKWMYLVNQIDILPFDIEVLAFFSEVIFLALYLYGWDRMSRTAHMIVGGLVGLGSSASAVLIILVNSWMNTPTGFNIEAYLQSGQLTGVDPLSATLPIIALAEVPMGLAGAWFVGFGSIAGYFAFRKLTKKDMTTDEKEYYNRGLKLATYLGALDAIFLGWAGDNAGKMLYLYQPLKLATLEGVMQTAAGAPMQVGPITIPNMLSLLATWPPNPHATVLGYSSFVQADWDPIWYISHTAYDLHATLGIIGAIAVWILAYSFWKQPKNALFKAFGLDNPAEKKIPLYAMFFLGWLQVVAWESGWVAAETGRQPFVIWGPMVQTASGLYEIQAVMLTADGFNNSPEVLPIGIAIMVVLALAVAGTIYMLKKLFTGKEVSADISSARLIMATNAGGSSSLNIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X2</accession>
    <name>F4B7X2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13 14">Adenylosuccinate lyase</fullName>
            <shortName evidence="14">ASL</shortName>
            <ecNumber evidence="5 13 14">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 14">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_0763</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 13 14"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775565.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X2"/>
    <dbReference type="STRING" id="933801.Ahos_0763"/>
    <dbReference type="GeneID" id="10600222"/>
    <dbReference type="KEGG" id="aho:Ahos_0763"/>
    <dbReference type="eggNOG" id="arCOG01747">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_2"/>
    <dbReference type="OrthoDB" id="26319at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01360">
        <property type="entry name" value="Adenylsuccinate_lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000008">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="15">
        <location>
            <begin position="357"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE93649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="456" mass="52184" checksum="C01628AA96961E43" modified="2011-06-28" version="1">MSICPLEWRYGSEEMRKIFSREGIIKYRIQVEIALLYALKDLGYVSQEDINIVEKVSENIDLNEIDKLEEKLGHDVMAMVVWLAEKSGESGRFIHFGATSYDIVDTAYALMFRDALNIIERKLLEILDTLSQMSIKYKDTIMIGRTHGQHALPITLGFKFANYVYELSRSLERIKDTERRLLKGKFAGAVGTMAAWGDKGRIIEEKVMQELGLTPHEISTQVAPRDGFAEIISDLAILASQLDRFALEVRELMRPEIRELSEGVGNRVGSSTMPHKENPVTAEKISGLAKVIRGLCLTELENIPLWHERDLTNSSSERIVLSHAFLIIDEMLDSMLSVLHNLIIYPENMKKNIEITKGLIMAESLMINLTLKGMPRHVAHELSMKLSRDAENNGRSLLEEAMENDVIKKYFTIDEIKRVLDPNNYLGQTEYLIQRAIKYYEKLKQEYNLKIQNTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A0</accession>
    <name>F4B8A0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Large ribosomal subunit protein eL37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpl37e</name>
        <name evidence="13" type="ordered locus">Ahos_0813</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775615.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A0"/>
    <dbReference type="STRING" id="933801.Ahos_0813"/>
    <dbReference type="GeneID" id="10600276"/>
    <dbReference type="KEGG" id="aho:Ahos_0813"/>
    <dbReference type="eggNOG" id="arCOG04126">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="OrthoDB" id="5619at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000003">
        <property type="entry name" value="50S ribosomal protein L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="11">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="61" mass="7073" checksum="5CD8000D8F848050" modified="2011-06-28" version="1">MKGTPSFGKMNKGATHIRCRRCGRNAYNPRKHYCAACGFGRSKRIRRYSWQNKKVNGVRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C2</accession>
    <name>F4B8C2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9 10">Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pfdB</name>
        <name evidence="12" type="ordered locus">Ahos_0835</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775637.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C2"/>
    <dbReference type="STRING" id="933801.Ahos_0835"/>
    <dbReference type="GeneID" id="10600303"/>
    <dbReference type="KEGG" id="aho:Ahos_0835"/>
    <dbReference type="eggNOG" id="arCOG01342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131909_2_1_2"/>
    <dbReference type="OrthoDB" id="204796at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23162">
        <property type="entry name" value="Prefoldin_beta_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000013">
        <property type="entry name" value="Prefoldin subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="9"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="15022" checksum="5A3641F8D359E988" modified="2011-06-28" version="1">MAEKIPPEVQTQLIKLQQLQKQLDNLTYERSVIDSELREINKILEELSKLSADTPVYKIVGNILVRKDKASIEQELNDRKEILELRSRTYLKQESLLRKQFEDLQKNVNDLLQRYYPQLKGSSNPPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C7</accession>
    <name>F4B8C7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF447 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775642.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C7"/>
    <dbReference type="STRING" id="933801.Ahos_0840"/>
    <dbReference type="GeneID" id="10600308"/>
    <dbReference type="KEGG" id="aho:Ahos_0840"/>
    <dbReference type="eggNOG" id="arCOG04458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406014_0_0_2"/>
    <dbReference type="OrthoDB" id="146030at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049288">
        <property type="entry name" value="DUF447_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20766">
        <property type="entry name" value="DUF447_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF447" evidence="1">
        <location>
            <begin position="17"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="DUF447" evidence="2">
        <location>
            <begin position="133"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22826" checksum="586BCFA44DCE4A5A" modified="2011-06-28" version="1">MELHEAIKNIFPSNGIYEVILGTKGIRDNISPIGVIVNENQLKVKLYKCTTTYQNILVYPYCSINVVIDNPKMFYLALFNKSVKYNLIHGLPVVSDNVIFSNCKIIEDHTEYVILSLDPFDVKYSRQKISAFNRGNCLFIDLLVNITRLDIFSREELESMLKVISYEIKVIKRTQPNLLDIIREIEDLIRSKGYKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C9</accession>
    <name>F4B8C9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="10">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ileS</name>
        <name evidence="14" type="ordered locus">Ahos_0842</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C9"/>
    <dbReference type="STRING" id="933801.Ahos_0842"/>
    <dbReference type="KEGG" id="aho:Ahos_0842"/>
    <dbReference type="eggNOG" id="arCOG00807">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000083">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="17"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="684"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="10">
        <location>
            <begin position="597"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1049" mass="122078" checksum="F7D22A439096EFA8" modified="2011-06-28" version="1">MKALSTKFDSKSIENEIIQYWNSNTIYEKLKKINQETRSKKFLFIDGPPYPSAPVPHIGTIWNKVIKDCILRFKRIEGYKVYDQPGYDTHGLPIEVAVEKKLGVVRKQDIIDKIGVDKFIQECRNFALTNAKSMTENFKNVGVFMDWNNPYYTLKNEYISNSWALIKKAYERGLLAKDVEVLHWCPRCETTLSDYEVSEYKDIEDPAIYVKFKVAGEDNKYLVIWTTTPWTIPANVFVMVNSQFDYAEVQVGNEIYIIAKDRVESVMKEAGIKDYKILRTYKGSQLIGLKYIHPLKDMVPAQKDLDEYHKVVDGGNEVTLEEGTGLVHSAPGHGDVDFEIGKKMNFPVVMLVNDKGEFLDIAGKYAGKYVRDANNEIIEDLKKVGALFHAGKIVHRYPICWRCKTPLILRAVEQWFIKVTKLKTELLNEIERVNWVPEWGKTRIGNMVKELRDWVISRQRFWGTPLPIWICKNGHITVVGSKEELQKLSVNGVPDDLHRPWIDNVIIRCPKCGEEARRIPDVADVWFDSGVAFFASLGENWDKKWKEIGPVDLVLEGHDQLRGWFFSLLRSGVILMDRAPYESVLVHGFMLDEQGREMHKSLGNYVEPSVVIEKYGRDILRLWLLRNTTWEDAKFSWKAMDLTMRDLQIVWNVYVFASTYMSLDNFEPEKYSFSDVEKYLRVEDKWILSRYYSMLRKIKESMKNYKVHEMANYLIDFIINDVSRFYLRLIRKRAWVEYNDPDKIAMYYVLYTVLKGWIILASTVIPYTAEKIYRDFVVNPKDSVSMEDFPEIQERFIDDQLEKAVDLVRKIEDAGLNVRAKAGIKLRWPVNKTFVFLNNEDDIKLVNSVIDMLKSVLNTKEVELHNIVDYAKFSQIIVSPLPGKIGKDYKQLTPKIVQYINNNTNVIGKDIIDKGYHEVVIDGVNVKLDKNYVNIEETSLEGYVSSKFDLGVIMISKTISEEEEEEGMIRDIIRRIQFMRKKLNLNVTDYINVSIMPPSDRVQMIEKWKNYIASETRAKNIEISDKKQELTESWDIEGEQYIIGISKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D6</accession>
    <name>F4B8D6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl22</name>
        <name evidence="7" type="ordered locus">Ahos_0849</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="4 6">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775651.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D6"/>
    <dbReference type="STRING" id="933801.Ahos_0849"/>
    <dbReference type="GeneID" id="10600317"/>
    <dbReference type="KEGG" id="aho:Ahos_0849"/>
    <dbReference type="eggNOG" id="arCOG04098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="OrthoDB" id="314984at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="156" mass="17666" checksum="3F4AC28F7D3A6601" modified="2011-06-28" version="1">MASWTYPQLSIDDTKLGKAVVKDAPVSIRDLYNVCKAIRGMKVKEARDFLDRVLKHQEALPFWRYSHGSSHRSNISSKWGIKNGRYPVKAIKYVLKALDNAEANAVSKGLDPDSLKIIHIAAHKSFTLKRFMPRAFGRATAKYRRTSHIEVIVGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F2</accession>
    <name>F4B8F2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribosomal protein L30P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F2"/>
    <dbReference type="STRING" id="933801.Ahos_0865"/>
    <dbReference type="KEGG" id="aho:Ahos_0865"/>
    <dbReference type="eggNOG" id="arCOG04086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="121" mass="13779" checksum="E6CB179D077DCAAF" modified="2011-06-28" version="1">MIYPYTESLKGMLIKVEPFITWGEISEDGLNALLNRLETCKGEKITEEYIKTKLSMDLNTFKTKLLSGELALNKLDNIFRLPIRLHPPSGGFKGKVNAPYKAKGEFGYRGLEINNLIKRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H8"/>
    <dbReference type="STRING" id="933801.Ahos_2059"/>
    <dbReference type="GeneID" id="10601559"/>
    <dbReference type="KEGG" id="aho:Ahos_2059"/>
    <dbReference type="eggNOG" id="arCOG05414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194227_0_0_2"/>
    <dbReference type="OrthoDB" id="37098at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="82" mass="9593" checksum="713622349CB594E1" modified="2011-06-28" version="1">MSITITIKVDRSIAELIEKMIKLGIAKSKNEAVNLLIEYGKAEIEKKIREEEKVEELVNKWLKEGFPYKHLDTSDLREERYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H9</accession>
    <name>F4B8H9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Chloride channel core</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_2060</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H9"/>
    <dbReference type="STRING" id="933801.Ahos_2060"/>
    <dbReference type="GeneID" id="10601560"/>
    <dbReference type="KEGG" id="aho:Ahos_2060"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0034707">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBS_dom"/>
    </dbReference>
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        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050368">
        <property type="entry name" value="ClC-type_chloride_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0868">Chloride</keyword>
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    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="192"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="304"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
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            <end position="333"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="390"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
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            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="519"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="EMBL" id="AEE94931.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="578" mass="62896" checksum="CA65C6848D46FA7E" modified="2011-06-28" version="1">MVKFSVDKLPYFEKWIILGTLIGIAVGLFSIAFTILLEFFQLLFIHIILHVSYPKPLGEGGNLRIPPFHPSFLIPAIVGLGGLIAGIIIYRISPEAAGGGVDFAITVYHKLQGKMRKRVAFVELFTSTIILGSGGSAGDLGPMGLIGGSLASTIAQLFDLTPEDMRRAVAVGIGSGVGAIFKAPIGGALLSAEILYRRDLEPDVILPSMISSAIAYSIYGSYVGFQPMFGLYPFQFSPLRLPFYALLGVIIGLFSILFVKIYSTITLVFKKMKIRDEIKPAIGGVLVGLLILIFPEVMGTGYGWDNLLEFGVIPFSSLPCIFLLLVGLVKMLASSLTIGSGGSGGIEAPAFEIGAFIGASYGLIVHDLFPNIVPVIAPFVIIGMLTMFGAPAKAPLSVMVIITEMTSSLQLLPGEMIAIALAYVISGKYTLYPAQYPTRRDSPAHRSEYEVPIMENIRVNEVPFTELRINENEDVMNARKLMEEDGYFSLPVVNNEGNFVGAVFYKDIANKKGKVIEYTVRGIPYVTPSSNIENAWEIITRTKSRWVAVVEKGKLIGVITLDDLFSAYERELKKVHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I7</accession>
    <name>F4B8I7_ACIHW</name>
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        <recommendedName>
            <fullName evidence="5">Thermopsin</fullName>
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    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2068</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I7"/>
    <dbReference type="STRING" id="933801.Ahos_2068"/>
    <dbReference type="GeneID" id="10601568"/>
    <dbReference type="KEGG" id="aho:Ahos_2068"/>
    <dbReference type="eggNOG" id="arCOG06001">
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    </dbReference>
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    <dbReference type="OrthoDB" id="34629at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="56818" checksum="54D4BE7E673F20EF" modified="2011-06-28" version="1">MRLIPIGIIALLLVPIFFMSIVNSSVSSNTYYTIEYNKYSSYLHIGKMGILSVEIYKVSNSTYYGNLSCNDKDTYVNHYTTIYTLVTGSAETINGKICINSSVSGNLYVGTNIPQLARIIVISSGMEEIVWAGFNETYVHTYSYINGSGEVILQFINGTSISNISIGIKQNSSIVQYVNLTLTKVSVSSESEYSSSFMLHVQMPRKYSPLSECFIANGSSFSSSAEGYTVTTAYFCGKLMPAIEWKGLGIGNLNLGLHAPMSGRVNFTTLEFYGINGTTVGYVHSAYINAEYHGIFEDSSFIAGELLIVHIVNATALAKPSLISVIYHQGVPVILGICANNTVETTANVVIQHQVYVNNAKGLLLMLSLNSSAFYVSVIHSTIVNVTIVKPTAVVVSNVTIQGKSYLAQIVNISASRYVTFNVSKVSNENFVVFEQESNGSLVELNSCDYFVVNGTIVVFTDPASTYLIVYGYTPTTSTSSTSSSSSPSAITSPSPSFSNTEILGIAIVVIILIIAVIMVIARKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J1</accession>
    <name>F4B8J1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cobalt transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J1"/>
    <dbReference type="STRING" id="933801.Ahos_2072"/>
    <dbReference type="KEGG" id="aho:Ahos_2072"/>
    <dbReference type="eggNOG" id="arCOG05945">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1492907_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20560" checksum="A62D5E1FCCEC1854" modified="2011-06-28" version="1">MLIFLSTYISLGIYAMVITITMLFRRKGIFIAEILITVFSYFILSYFDKAYLYVFTVRAITYINLYFIISELVDKSTVLDALGEKGVPIIIALAYYPYFFQLSSEVSFYAKARGIGFNPIKISRPLLVEMIKVAENLYVAYTVKLFGNYSGKRNLKPRTSDLILLTIGVVIFILSLVFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L0</accession>
    <name>F4B8L0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aminotransferase class V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776877.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L0"/>
    <dbReference type="STRING" id="933801.Ahos_2091"/>
    <dbReference type="GeneID" id="10601591"/>
    <dbReference type="KEGG" id="aho:Ahos_2091"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_5_2_2"/>
    <dbReference type="OrthoDB" id="35685at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="8">
        <location>
            <begin position="63"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="395" mass="43226" checksum="9C321CC7B48D11B8" modified="2011-06-28" version="1">MTRKILMHVGPETIDYDILLGGIKGDVGFTSKEFVDAMSFSLKFLRKLMGVGESYQPFIIPGGGTSAMESVTSLLRKGDKVLVVSNGVFGNRWIDIFSRYPVNVKALKAKAGYYVKPEEIEEEVKKDHYTMVTMTHVETSTGVREPIAEVTKRIRDYVDLIVVDGVASVGGEEVRAEDWKIDVFLTASQKAIGTPPGAGLLVLSENATKRLGEDNIAGYYLNLKNWIGVMQSMEKGKASYFSTLPVHLILMIKKAFELIETEGIGNRIRRHEIVSSAIRKGIEALGLEIVAKTPEAYSNTVTGVMLKKVNPNDVLSMSLTEGIEFAPGVHPDLGGKYFRIGHMGWVNINDVISAIAVIERVLSKLGEPINFGEGVKAVQEYLINGISISSSNIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R1</accession>
    <name>F4B8R1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3834 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775697.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R1"/>
    <dbReference type="GeneID" id="10600367"/>
    <dbReference type="KEGG" id="aho:Ahos_0895"/>
    <dbReference type="eggNOG" id="arCOG03693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1307872_0_0_2"/>
    <dbReference type="OrthoDB" id="56574at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024533">
        <property type="entry name" value="DUF3834"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12916">
        <property type="entry name" value="DUF3834"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="25799" checksum="F1289DE6572691C8" modified="2011-06-28" version="1">MLVTAPFAGPVSFPLLVARELGEEQINLKNSCETNEIGDIVLDSITNIAKLKVKGYKIVAGVYIDMYSVLGNKNSTRIYTIRAGTLADINARLYAKYTNKEVINTDAETAVKKSEEGYLAIVGIEVKIGEKLEDELARLGLRTPSCVMYSKVDPTNLLKIYEKGINIIREDPKNSARVISSLSKYYTLNVMENIIEHYNHMLTINFHELKKSIEVYSQIVPSVSDLQLGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S4</accession>
    <name>F4B8S4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </evidence>
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        <source>
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        </source>
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    <sequence length="329" mass="37128" checksum="6B6F0FAE8AFC6A80" modified="2011-06-28" version="1">MRLLLKLAISWILWGIGFYLYYPFLSIFLVRYIKPSELGFFYLTTLIFSLPLPYLGGKINKKFGDKYSIILGMSISGTGILLLPFASSIILLYIFMIMYNSFYIALPAFYELMEIEGKSTISKVWAISVLPSIFMPSIGGMISQYLGFTYLFIISAFFIIFSFLPLITQKEIKNTDPKIEFYPIISLIILPVALSSQFIYLVIKEMYNLSDTLIGIIATFAELLGSIIAFTSSFISSKKAINLSLILFSLQSLIIISPYFSAFYGLWESIIPLSLSISKKNYVSAVTMQMIGWILGYLIASIIQSPRDAIFLSSVLSLIVLAFINFGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
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    </evidence>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORF D-335-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="89" mass="9502" checksum="B1B2DCFE1E101AE8" modified="2011-06-28" version="1">MGNKVFTFGDIRIREVKGKYYVYLIEKGDGGQRRDHYIGSLDKIIETYVSIGGVGVPPTVRGAGFEPARAYASGASVRPLGPSSGIPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z3</accession>
    <name>F4B8Z3_ACIHW</name>
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        <submittedName>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776931.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z3"/>
    <dbReference type="STRING" id="933801.Ahos_2145"/>
    <dbReference type="GeneID" id="10601648"/>
    <dbReference type="KEGG" id="aho:Ahos_2145"/>
    <dbReference type="eggNOG" id="arCOG01727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_5_0_2"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053655">
        <property type="entry name" value="HexPP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040936">
        <property type="entry name" value="hexpp_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF85">
        <property type="entry name" value="SHORT CHAIN ISOPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="282" mass="32002" checksum="8799ECC588230435" modified="2011-06-28" version="1">MDLIEFWERSKVIIDSLVTKFVDDVKDWEIMEMSKYIMKDGKRFRGTLVFLFNDALGGEEKNAYQGALATEILHSASLALDDIVDYDLTRRGMASAWAVYTNRRVIFVSNFLIPTALKIISSYGKEALNISIELWKDTAIGALKDMYGDKREYFKTIELKTASLFKLPAMLASFSSGKKELVDKLLEAGRVLGVIYQLIDDYVDCVKLEPEKLVGSAKQLFELTNGKVDPFVSNKYAELKSEYLRIISSIPFFNEYNELIIALPDFLTMGLLAESGIKNKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B914</accession>
    <name>F4B914_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776952.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B914"/>
    <dbReference type="STRING" id="933801.Ahos_2166"/>
    <dbReference type="GeneID" id="10601669"/>
    <dbReference type="KEGG" id="aho:Ahos_2166"/>
    <dbReference type="eggNOG" id="arCOG00217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="OrthoDB" id="31371at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011067">
        <property type="entry name" value="PRK14497.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="182"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="538" mass="59833" checksum="4E1AAF4E8D81FA75" modified="2011-06-28" version="1">MRAILPEDSLLSAQEAISLFVSRVPPERRIKEIEITDALNKISAEDVYSPIDLPPFSRSTVDGFAVKSSFTPGKFKIIGKINIGEYKKIEISDNEAVEVDTGAMIPKGADAVIKIEETKIEGEYVEIPKKLNFGTNIAWIGSDMPRGTQILYKGEKITPEKIAFLASAGIGKVKVYEPPSVYVISTGDELIEPGKELQPGKIYESNLHFLISRLKNDGYNVVGHKVVGDDKDKIEEALEEASEIADVIIITGGTSAGEKDLVHQIIREKGEIIVHGLKFKPGKPTLLGKFNGKPVFGLPGNIVSTIMIYDRVIRNYLSLMKGEENTTEETEYAQLLLPVKADKKRFTYIPVYLVQGFAIPIPFDSYMVGSFSLADGFIGLEPGTELKEGDKVEVIIKRKDMRITLIGEEDKRFDNLNVRKIYLGSQVSCKALEKGIGDIVVVSSLFCTPNNYDYLIERRILEQGEGDEVGYFDWLGLSKFNRNPSVKLKSPSTVLNFLDKAKVFAPEGYLKEGKELGKEKLFVIVRNKEAFKFLNGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B928</accession>
    <name>F4B928_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NMD3 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776966.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B928"/>
    <dbReference type="STRING" id="933801.Ahos_2180"/>
    <dbReference type="GeneID" id="10601683"/>
    <dbReference type="KEGG" id="aho:Ahos_2180"/>
    <dbReference type="eggNOG" id="arCOG04149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065087_1_1_2"/>
    <dbReference type="OrthoDB" id="15051at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="234" mass="27305" checksum="129F08831CAA3334" modified="2011-06-28" version="1">MPKKFCIVCGREDVELIDRMCPSCYIKTKRLVEFPPVILGKICKICNAEWIDGKWVRLAETEYDAVRDVVLRYLAKEMIIDRNVKDYRIDMIKKWKERGGRSFAEIVVEGKVGNQAFRLKKKVELRLTYSICDDCLKRKSGYYEAIIQLRGRKEFSEEKRALFESFFTNETVHSISDVKISRDGIDYYFINKTAAKRLISTITSIVNAEITESYENETIKNGKREAKLVISVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C0</accession>
    <name>F4B9C0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Malto-oligosyltrehalose synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775829.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C0"/>
    <dbReference type="STRING" id="933801.Ahos_1027"/>
    <dbReference type="GeneID" id="10600502"/>
    <dbReference type="KEGG" id="aho:Ahos_1027"/>
    <dbReference type="eggNOG" id="arCOG02955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005045_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047470">
        <property type="term" value="F:(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030980">
        <property type="term" value="P:alpha-glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11336">
        <property type="entry name" value="AmyAc_MTSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1590.10">
        <property type="entry name" value="Maltooligosyl trehalose synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.200">
        <property type="entry name" value="Maltooligosyl trehalose synthase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.470">
        <property type="entry name" value="Maltooligosyl trehalose synthase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013797">
        <property type="entry name" value="Maltooligo_trehalose_synth_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012767">
        <property type="entry name" value="Trehalose_TreY"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02401">
        <property type="entry name" value="trehalose_TreY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF216">
        <property type="entry name" value="MALTOOLIGOSYL TREHALOSE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="1">
        <location>
            <begin position="13"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="728" mass="86318" checksum="822653776CA40237" modified="2011-06-28" version="1">MSYRIQLNKEFNFSNLISILDYLHDLGVEWLYLSPILQARKGSTHGYDVYDFKKISEDLGGEEKFLELCKEARKRGMKIIVDIVPNHMALENPYLLDFLRNSNSKYRKFFDFDGDKIVLPILGEEDLSKLKIVEEKGEKFLGYQGLKLPLIGEGEDIKELLKKQNYELVYWKRFDKINYRRFFDVNGLIGIRQEDEEVFNEYHEKIFELKDCIDGLRVDHIDGLLNPRKYLEMLREKMGNDKYIVVEKILSPKEKLRDWKIDGTTGYDFMRDVNLLFVNSKNEGKFKEIYEDFVGQKIDLEEEKIKAKIDVINQLFYGDVDRILRKLKELTGREIKKESLIKFLSCLNVYRTYITEDEIEWEDIEEIKECADESILDLINERKGMMILQQLEDPIMAKGYEDTLFYRYNLLVSLNEVGSDLKFGISCEEFHARNMERELFWPNTMIDTSTHDTKLSEDARSRINALSNFPEEWRNLIKRWSTINEKYKDKGYPIRNDEYRFYQVLLGTWNGYSKEYENRLVNYMIKAIREEKINTSWLNPNQEYEDATINFVRRVIKDEEFLSSFIPFFKKINKVGSIYSVEQVIIKLTSPGLPDIYQGNETLTYLMVDPDNRRKVDFNTLAEMLKEVKSEDPVTLFKNEEFGKLKLYVTWKLLNLRRDRKELFKGYRPIKLNGCGFERNGLITLVTFYPFENFEVKVKGKYIGIIDSSVHEGVIKSRELPFGVYVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D4</accession>
    <name>F4B9D4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydropteroate synthase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D4"/>
    <dbReference type="STRING" id="933801.Ahos_2209"/>
    <dbReference type="KEGG" id="aho:Ahos_2209"/>
    <dbReference type="eggNOG" id="arCOG01978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041129_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005236">
        <property type="entry name" value="Dihydropt_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045406">
        <property type="entry name" value="DUF6513"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025595">
        <property type="entry name" value="PterinBD-DUF4346"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00284">
        <property type="entry name" value="dihydropteroate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20123">
        <property type="entry name" value="DUF6513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14251">
        <property type="entry name" value="PterinBD-DUF4346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="152"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="508" mass="57369" checksum="88A409F150533989" modified="2011-06-28" version="1">MYLEYIDLKVNYSWNIVKALLITGKLAYPIVKEVAKKIKNVKAEVKALNYPVASLMSVQYILENIKNDNLKDFDIIVLPGLVFGDAKIIEEETGIKTVKGTENAWDLPLVFDALNQGIELSTVYPADKILGKIRTEKVLKTLEEIEEKQEVAFEQGIKIPLRPPPFRVFLELDPTWSIERILEEIERTKEFVDIYVIGFPVGHDDLDDVRNKIRKAKDSGVIVGIDSDSPREMIEGIKEGADFVFNINEVNYKKLEEVKNKAAFVVAPFNVENKSEIVIKLTKILSSKGFTKLILDAVLSPPLQGMVDSFIEYKRIREALPQYPLLMGTLNVTELVDADSHGMNALLTAIAGELGISCLLTMEKGKTRWSSWEIKEASKMVSVSLIQKRAPKDLGVDLLILKDKVRYKEEEEELKDFITVEHNEPEMDSAGFAKISLVNGKIALTFYGKDKITLVGDEALSIGRELVKRVNISKQHAVYIGYELAKAEIARNLDKNYIQDIPLFKKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H1</accession>
    <name>F4B9H1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MscS Mechanosensitive ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2246</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777032.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H1"/>
    <dbReference type="STRING" id="933801.Ahos_2246"/>
    <dbReference type="GeneID" id="10601752"/>
    <dbReference type="KEGG" id="aho:Ahos_2246"/>
    <dbReference type="eggNOG" id="arCOG01573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893171_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="2">
        <location>
            <begin position="134"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="300" mass="32424" checksum="BAE52DA38275F0BC" modified="2011-06-28" version="1">MLNLQIKWKRQVVKLLSMIVVLAIIAVLLHFLIEFASSNFPVIVPYLNDIYLATDAAIVGIGGYFIIGILKRIINVYLLGKMEASTARTLSFFIDIALYSILILVILAALGVNLTGAAIGGAVGGIAIGLAAQTVLSNVLSGALVTGSKTLKAGDAVLLISWIWGSPIIGETTKVGVLFTEVKTINGNIVKVPNSAFLGNTVFTKLEGENSLVYPLQITVNADVPADKVMEKAKNYIKDELSKQKIPYPEIYFTNKTGGTNVFTVILHFDNLNMLNSLLSVVNNAFDKAYWDTKNEVSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P5</accession>
    <name>F4B9P5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1077</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775877.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P5"/>
    <dbReference type="STRING" id="933801.Ahos_1077"/>
    <dbReference type="GeneID" id="10600551"/>
    <dbReference type="KEGG" id="aho:Ahos_1077"/>
    <dbReference type="eggNOG" id="arCOG00664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197:SF1">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="251" mass="28500" checksum="9EE203F7A45D4E60" modified="2011-06-28" version="1">MIRKAVITAAGKGSRMRYITSVLPKALLPLFIQEDGKKVMRPIIDLIMSSLSAVGVNNFCIVVGKHGKLLMDYLFERGVTFVFQEEPKGFGDAVLRAENFACGEPFFVHADDGVLTGGYSEASELYEENKPEAVLLLRKVNNPFRYGIVKAEEKGEFRNHKLYKIIDVQEKPKQPFSDLAISAVYIFKPSIFKALKEVKNYEGELELTYGIKKILDQGGEIYAILLEREKWLNVGDPEHYYEALKYSFEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q6</accession>
    <name>F4B9Q6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775888.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q6"/>
    <dbReference type="STRING" id="933801.Ahos_1088"/>
    <dbReference type="GeneID" id="10600562"/>
    <dbReference type="KEGG" id="aho:Ahos_1088"/>
    <dbReference type="eggNOG" id="arCOG00322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_2_2"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047657">
        <property type="entry name" value="PmbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421:SF1">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="1">
        <location>
            <begin position="15"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="3">
        <location>
            <begin position="107"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="2">
        <location>
            <begin position="208"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="415" mass="47396" checksum="73410E7415040A8B" modified="2011-06-28" version="1">MFEILDKAKNQGYSAEIFYVKRTEFSINEEKQIYSRFLNEEGYGLRVFKDGKVGFAYSTRLDENLLDNAIKSWKVSERDDANQIPTPNKVNYLNLYKKFDLSEVLKEKIRELEDLKEKKINIVTLNSTAWVSEVGIISTEGMNLSEKRSGISVSVSANYKDSTYVGPEIYETRSFRDPSTSIDEMKDTIIEKVQITSERTTLDFKPKSVILTPKAVYYLVFPLLSHAISLENYYRNRSPLKEGELINEKLRIVDNPLIENSVYSRSFDGEGQQSLNNEIINANVKKFLSNYYWSIKAKRENTASASRGYSSIPYIYPSNLEFYVKNEEEDLSEEGKVYVDEVQGVHTSNFDTGEFSVVGSVSWIIKDGKKISLKEIVVTSNLKDLLKGIQRTSKKRLMIGNVSTGELEIQGLSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S6</accession>
    <name>F4B9S6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1 2">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name evidence="5" type="ordered locus">Ahos_1108</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1 2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775908.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S6"/>
    <dbReference type="STRING" id="933801.Ahos_1108"/>
    <dbReference type="GeneID" id="10600582"/>
    <dbReference type="KEGG" id="aho:Ahos_1108"/>
    <dbReference type="eggNOG" id="arCOG01748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_2"/>
    <dbReference type="OrthoDB" id="27337at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="4">
        <location>
            <begin position="58"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="445" mass="50108" checksum="A183095B4CBD60F2" modified="2011-06-28" version="1">MLYRKWGSSGDEVVSFTSSIDSDKEIIEEVKLTMKAHVIELYLNKIISKEIAGKIISAINDFKEIPSGYEDVHEALEDYIIKKVGEEGGWIGLGRSRNDHVATALRLKAREELLSILDEIIKLREVLLNKAEQSLDVIFPAFTHLQPAQPTTFGHYLSYIEEELYSAWEIIFSNLKLVNRSPLGSGAIVGSTVNINREREAEMLGFDDIITNTISASSSRIDLISSVSGMVILSLILSRIAEDMVIFSSMKLVKLPDNQVSTSSLMPQKRNPVTMEIMRAKAGELIGFFTSLSSIYKGLPSGYDLDMQEMNKYYWYSFDYVKNSLSVMKSLFENIEVLPSALDNSMLATDEAESISLQGIPYRKAYFEVSSKVMKGTFTPSINFKESINLKKTEGSPNPEILKNDIKKAKDRIEENKMRLNEFKTKISNKISQLKAIENDLQEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T8</accession>
    <name>F4B9T8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">taw3</name>
        <name evidence="9" type="ordered locus">Ahos_1120</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T8"/>
    <dbReference type="STRING" id="933801.Ahos_1120"/>
    <dbReference type="KEGG" id="aho:Ahos_1120"/>
    <dbReference type="eggNOG" id="arCOG04156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047426_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003263">
        <property type="entry name" value="PRK04235.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="24668" checksum="4CFE781CA3C63A22" modified="2011-06-28" version="1">MLYKLMEYIKSGMDDWELWKKKAYERMIRDKEIGYLDPDIYDVLVAFFKREKSFTYSSCSGRITIIDSYLPWIRKNSTIIFKNHLGITEDDVKDILEKPQVYRLWLVSQGPILHVYTKDWEEAWDIIKIARNAGFKHSGVLTVNNKGILVELKTGIKLVHLLRENSEQQVSEDQIKTIVKISNEVLKKGKDKLWELQKLVEESLKTKVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U6</accession>
    <name>F4B9U6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mre11</name>
        <name evidence="11" type="ordered locus">Ahos_2291</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777077.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U6"/>
    <dbReference type="STRING" id="933801.Ahos_2291"/>
    <dbReference type="GeneID" id="10601799"/>
    <dbReference type="KEGG" id="aho:Ahos_2291"/>
    <dbReference type="eggNOG" id="arCOG00397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_5_2_2"/>
    <dbReference type="OrthoDB" id="11638at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053459">
        <property type="entry name" value="DSB_Repair_Mre11/Rad50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041031">
        <property type="entry name" value="Mre11_Sulfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
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    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
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    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="194"/>
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        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
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            <position position="10"/>
        </location>
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            <position position="49"/>
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            <position position="49"/>
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        <ligand>
            <name>Mn(2+)</name>
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        <location>
            <position position="83"/>
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            <dbReference type="ChEBI" id="CHEBI:29035"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29035"/>
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            <dbReference type="ChEBI" id="CHEBI:29035"/>
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        </source>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF00149"/>
        </source>
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        <source>
            <dbReference type="EMBL" id="AEE95162.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="377" mass="43342" checksum="0277ACE14BBA2EE4" modified="2011-06-28" version="1">MQILHISDTHLGARKYNLDSREEDIYETFTQLIDYAIKEHVDAIIHTGDLFDTYHPQMSAMKVAIDNLKRIQGKIPFISIAGDHDTPKRRGAIYPQRILAESLNLLVFLTGDEKGYEINKDNIRLRIYGIKHIPTVARETLLKKLSSIKPEGDRNILMLHQGLRSKLPYEGAWQLEEGELPKGFNYYAFGHFHSRSIENLGDGKLGIAGSPEIIRDDEIEAWEKDGKGGYLIDLSKKEIEIQKVNTDIRPQKIVTINTKNLDNEINNLMRIFSKYKKKPILHIILEGEAVSKSLLFKKLSILESVTEFYRIAKDNTTYGEEKNVELPKDSTISQIIMTYLKNFGYTEEESKLILEIINNYDSDDIYTIIKKFAGVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="GeneID" id="10601843"/>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="109"/>
            <end position="113"/>
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    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE95206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="157" mass="18161" checksum="1C9367F7FDDE115A" modified="2011-06-28" version="1">MTDINLSKKVVLSNMCLFCKIAKKEESAYVVYEDEYTVAFLDKFPLAPGHTLVVTKEHFDDFLNTKKEYLEKLAFSSNVVSRAVKESVKSSGIRLLTNVGKSAGQVIFHVHIHIIPTWDGSYPEEFSYFEPRKEQRKEYYEFLQRVISQNVKNILSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z5</accession>
    <name>F4B9Z5_ACIHW</name>
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        <recommendedName>
            <fullName evidence="4">DUF2029 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z5"/>
    <dbReference type="STRING" id="933801.Ahos_2340"/>
    <dbReference type="KEGG" id="aho:Ahos_2340"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_551680_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="107"/>
            <end position="124"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="255"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="267"/>
            <end position="284"/>
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            <end position="310"/>
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        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="58978" checksum="DE2286F42A55C53D" modified="2011-06-28" version="1">MNNPVTAAVLALSFFSAIAIFVIALDPYILNPNRKINMIDDTIVTISILTYTLISIFLINGYGTDDMEYIATAINYLIHGINPYLQSYFPHNVEPTYLLNGNIASNYIYPPLSFLLYAPLYLILDLFKIKLYYINILNIIFEDLLAIIIYSQGRKRKDPIATLPIIFIFITSGLLAPSFAGVNSSVWAVFIALSYVYNGKKSGIFLALADSFNQIPWLITPFLLIYKKNDLFNVLKGFLISVLLINVPFMIWNPYAFLHIITLDEKTIPVAFTGFTILNFTTLFSVEPWFFTYAMALSGAFLLYIYYRFFNRLKESLWIFPLIIMWFSWRTLTSYFIMWPQLMFLSIFNINSYNTEIPKISLSINRKEILSVLFVLLISLVSAGEFSHIQYVDQDPIQIINVIIPESEHNSTYINQIYIVVKNIKNETVNITLVRVSIPNCLNMVWNFTKVEIPPNSTGVIFAYTQNPALYINSTSFTVQVYSNCYISSYKVIRNFTEYNNTLIYESSISASGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA30</accession>
    <name>F4BA30_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoribosyl-AMP cyclohydrolase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1143</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775943.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA30"/>
    <dbReference type="STRING" id="933801.Ahos_1143"/>
    <dbReference type="GeneID" id="10600617"/>
    <dbReference type="KEGG" id="aho:Ahos_1143"/>
    <dbReference type="eggNOG" id="arCOG02676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_1_2"/>
    <dbReference type="OrthoDB" id="5853at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000001">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000768">
        <property type="entry name" value="PRK00051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="7">
        <location>
            <begin position="42"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14753" checksum="A78C23FB7F971447" modified="2011-06-28" version="1">MGVTLKLSEDEAKKIIEKLNFRHEYDTIIAILQHYKTKDVLMVGNMNKEAVFKTLTTGMAHFWSLSRKRLWLKGETSRHFQLIEDFYVDCDEDAIVFLVNPLGPTCHTGNYTCFYRNYRSFITKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA45</accession>
    <name>F4BA45_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775958.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA45"/>
    <dbReference type="GeneID" id="10600633"/>
    <dbReference type="KEGG" id="aho:Ahos_1159"/>
    <dbReference type="eggNOG" id="arCOG08842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_198715_0_0_2"/>
    <dbReference type="OrthoDB" id="35904at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054264">
        <property type="entry name" value="PBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22511">
        <property type="entry name" value="PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="63" mass="7277" checksum="853D40439D593082" modified="2011-06-28" version="1">MEKDIEIAEKYFRKYISVGEIIAVRDLKALGVKEPEKVIVELMNKGIIEKGEGCFNLVREKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA57</accession>
    <name>F4BA57_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosomal protein S26e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775970.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA57"/>
    <dbReference type="STRING" id="933801.Ahos_1171"/>
    <dbReference type="GeneID" id="10600645"/>
    <dbReference type="KEGG" id="aho:Ahos_1171"/>
    <dbReference type="eggNOG" id="arCOG04305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129451_3_0_2"/>
    <dbReference type="OrthoDB" id="51292at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000004">
        <property type="entry name" value="30S ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006816">
        <property type="entry name" value="PRK09335.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="10877" checksum="1BFDC8DE1539D31C" modified="2011-06-28" version="1">MPKKRENRGRRKGDKGHVGYVTCDNCGARVPEDKAICVTRMYSPVDAALASELEKKGAIIPKYPVRKCYCINCAIHFGIVKIRAEEERKTKPTTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA63</accession>
    <name>F4BA63_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">A-type ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpE</name>
        <name evidence="10" type="ordered locus">Ahos_1177</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775976.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA63"/>
    <dbReference type="STRING" id="933801.Ahos_1177"/>
    <dbReference type="GeneID" id="10600651"/>
    <dbReference type="KEGG" id="aho:Ahos_1177"/>
    <dbReference type="eggNOG" id="arCOG00869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1412391_0_0_2"/>
    <dbReference type="OrthoDB" id="43245at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="197" mass="22563" checksum="984A0038579C8632" modified="2011-06-28" version="1">MELNDLLDYAIKKVMEDIRENLEQALAEANKIIENKYSDILSDYDKKIRDLISKRREQIEGEKAKLDVENKRAILNEENYWLQKVYDEVVKNINVVTSSKEYAEGLQSIIKKEISSSTTKAKIICNKSDEELVKKILKDNKLSADVEEDNSLLGGIKIYYPDVGLVRDYSLNLILSQVFESVKPKVAEILFGGENGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA72</accession>
    <name>F4BA72_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA polymerase</fullName>
            <ecNumber evidence="8">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1186</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA72"/>
    <dbReference type="STRING" id="933801.Ahos_1186"/>
    <dbReference type="KEGG" id="aho:Ahos_1186"/>
    <dbReference type="eggNOG" id="arCOG15272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05783">
        <property type="entry name" value="DNA_polB_B1_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000011">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004415">
        <property type="entry name" value="PRK05761.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004417">
        <property type="entry name" value="PRK05761.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF20">
        <property type="entry name" value="DNA POLYMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="10">
        <location>
            <begin position="164"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="9">
        <location>
            <begin position="495"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="885" mass="102112" checksum="BB8520214E76B075" modified="2011-06-28" version="1">MLKEYFFHLYNVAKQITLFDFSIKQETHSEGKGVEKEEEVQQLEVENKRGQVEWIKEAKEGRTYYLLGVDYDGKKGKAVMKLYDPETQEAAILYDNTGHKSYFLVDLDPEKVRKIAKIIRDPSFDHLEVVTKIDPYTWKPITLTKIVVKDPLAVRRMRNYAPKAYEAHIKYFNNYIYDMQLIPGMPYKVNKGRLEAIIPKVDTEEVTKAFQDSDEVTKEMALQWAPLFESEIPSIKRVAVDIEVFTPMKGRVPDPYKAEFPIISISLAGSDGLKKVLVIEREDVGEGNTNNEGISVEKFKTEREMLSRFFEIVKTYPLLLTFNGDDFDVPYIYFRSLKLGFFPEEIPFDIGSRETKFLPGFHIDLYRFFFNKAVRNYAFEGKYSEYNLDAVASALLGMSKVKVDSLISDLDISKLIQYNFRDSEITLKLTTFNNDLTWKLIVLFARISKLGIEELTRTEISAWVKNLYYWEHRKRNWLIPLKEEILQRSSTLKTSAVIKGKGYKGAVVIDPPVGVFFNVTVLDFASLYPSIIRTWNLSYETVDIENCKNIVDVKDETGQVLHHVCMDRPGITAVITGLLRDFRVKIYKKKSKQKDLDPERKMMYEVVQRGMKVFINATYGVFGAESFPLYAPAVAESVTALGRYVITSTVKKAQEVGTRVLYGDTDSLFLYQPTQEQLNTIIKWVKENFKLDLELDKSYRFVAFSGLKKNYFGVFTDSTFDIKGMLAKKRNTPEFLKEAFKEVKDLMGTINSPSDLEKVKKEVAEKVKSVYDRLKNKEYNLDELAFRVMLSKDLDSYTKNTPQHVKAAMQLRALGIQVLPRDMILFVKVKSKEGVKPVQLAKLTEIDLDKYYDAVKSTFEQLLKSLGISWDEIASTISIDSFFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA78</accession>
    <name>F4BA78_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH-ubiquinone/plastoquinone oxidoreductase chain 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775991.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA78"/>
    <dbReference type="STRING" id="933801.Ahos_1192"/>
    <dbReference type="GeneID" id="10600668"/>
    <dbReference type="KEGG" id="aho:Ahos_1192"/>
    <dbReference type="eggNOG" id="arCOG04654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582963_0_0_2"/>
    <dbReference type="OrthoDB" id="37055at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33269:SF17">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="164" mass="17922" checksum="230AC3F90FE0B0C2" modified="2011-06-28" version="1">MFQSLNLCLILFLFFSIISISSAIFIVSSKNLFYAAISLAFLGVSIAVLIALISFQYSLYSVFHLLLYVGATVVFLSISLVMFKGLEVRQINIAWAPIVAGVSAVLIFIAVVLSLSEVQLGQVGEINLQTLSSIILEKYWFPAVVLVVGLLTTLIEAITLARRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M2</accession>
    <name>F4B3M2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774828.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M2"/>
    <dbReference type="STRING" id="933801.Ahos_0016"/>
    <dbReference type="GeneID" id="10599456"/>
    <dbReference type="KEGG" id="aho:Ahos_0016"/>
    <dbReference type="eggNOG" id="arCOG05913">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431570_0_0_2"/>
    <dbReference type="OrthoDB" id="36571at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="189" mass="22294" checksum="2A5EF7AA8476FA0B" modified="2011-06-28" version="1">MSYDERTTAEAVIEIHFNLYEFRDSISKYTIVSDDDWQIISGKEGKYMTKEFDTYGILMYPIETSEDIKYAFSNKIEKIDKFREALYKPRYWREFITIMIEGNKLYTSPDLGLQIFNGVDLVNDIARSKGIQYEDIDDYLRISIDLTRPLTKESLNDAMERLSKALLLYYRIKEYQEDVASKLASQMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M3</accession>
    <name>F4B3M3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0017</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M3"/>
    <dbReference type="STRING" id="933801.Ahos_0017"/>
    <dbReference type="KEGG" id="aho:Ahos_0017"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067021_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="10">
        <location>
            <begin position="38"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE92912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="347" mass="40096" checksum="B73AEE22384123F9" modified="2011-06-28" version="1">MILLSSILLDLGILIQIYAENKNFFKEPKGGNYHGNISVIIPIRGIDVNFEENVKSLLNQDIKPLEIIYVVDPDDPYRETIENILKKYNVKIVHTFYNCNTCSGKIRAQISGLLRSNGDIIVFGDSDTCYPKYWLRELTLPLSKYMATTTFSFASPARLTLKNLVRAGFWTLGIESQAIGGTFLWGGSMAFRRNFFNDHVIKSLSKEWCDDCTLTRIVKERKGKIGFIGNAIPLNVYDEHNLFKWAQRQVLTVKIYSYRGVRAFIPFGLYMIILLISLILIKNFLLLLPFLLWIIKNLLRGRRLGRKAILPSLLSIIGIYFAWIVLILSWRKKEIIWRDKIYTVENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S7</accession>
    <name>F4B3S7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytotoxic translational repressor of toxin-antitoxin stability system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776030.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S7"/>
    <dbReference type="GeneID" id="10600708"/>
    <dbReference type="KEGG" id="aho:Ahos_1231"/>
    <dbReference type="eggNOG" id="arCOG01665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157019_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="10344" checksum="875613172CD745E8" modified="2011-06-28" version="1">MDRKEFIGWLKRNFPQSKLLRLILEKLELMNEDPFKYAREKLGTDKYGNPMFSIDVTGDIRILYSVDSKNCIVFIWEIGSHKKVYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T5</accession>
    <name>F4B3T5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
            <ecNumber evidence="10">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cca</name>
        <name evidence="14" type="ordered locus">Ahos_1239</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776038.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T5"/>
    <dbReference type="STRING" id="933801.Ahos_1239"/>
    <dbReference type="GeneID" id="10600718"/>
    <dbReference type="KEGG" id="aho:Ahos_1239"/>
    <dbReference type="eggNOG" id="arCOG04249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044679_1_0_2"/>
    <dbReference type="OrthoDB" id="7378at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160016">
        <property type="term" value="F:CCACCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048833">
        <property type="entry name" value="CAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643:SF1">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21133">
        <property type="entry name" value="CAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50152">
        <property type="entry name" value="25A_SYNTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 10 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0692">RNA repair</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase substrate binding" evidence="12">
        <location>
            <begin position="146"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="CCA-adding enzyme C-terminal" evidence="13">
        <location>
            <begin position="275"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="417" mass="47980" checksum="286D16BF52F461B9" modified="2011-06-28" version="1">MSEELLKKVLDKIKPTREDEEKVKLIVNEVIEKLNGLDAEIHGSFRKGTWLKGDADVDVFVFFPKNVGKEYISTEAIKILRDRLSGFDITTAYAEHPYLIVNHKGIEIDIVPALKIEEGEKPITAVDRTPFHTEFINSHLSEEQKDEVRLLKRFMKGIGVYGAEIKVRGFSGYVTELLVVKFGSFLEVLKNSSKWKPPVKIFIVKPERDFKCPLILPDPVDPSRNAGAAVSLKRLAEFSIASRYFLKNPSEKFFFPPEPAGDKIKGDVLVTKIEILDNNVVEDLLWGQINKSVEKIFNILRNYGYRVIDVQAYGDVKNLLFALQLESKEIGNYKLNQGPPFYMNLDDFISKNDNIWVGEDGRLYSIKERRNDKIEDIVKSAISLKYNYKVVEQYWLDREPKEPCLKAFLRKTPTWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T8</accession>
    <name>F4B3T8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein Ahos_1242</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776041.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T8"/>
    <dbReference type="STRING" id="933801.Ahos_1242"/>
    <dbReference type="GeneID" id="10600721"/>
    <dbReference type="KEGG" id="aho:Ahos_1242"/>
    <dbReference type="eggNOG" id="arCOG01043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134829_1_0_2"/>
    <dbReference type="OrthoDB" id="7819at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001687">
        <property type="entry name" value="PRK00447.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652:SF1">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="145" mass="16727" checksum="36FC45EFC40E57EF" modified="2011-06-28" version="1">MTKIVVTAEVRPSEDESKVLMAIANFFDYEKITKERRGDYIVVIVEESRTLKSLQKFHDALREEKILDVARKYLRRGISDNSISFMLHKQAAAIGVISFVDDERESPLGPISFYIEHKNPNEVVDWLAPRTAKGHPLWENKIPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X6</accession>
    <name>F4B3X6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
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</entry>
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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</entry>
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                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
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    </dbReference>
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    </evidence>
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    </evidence>
    <sequence length="61" mass="7017" checksum="EB735BB2AC3E3431" modified="2011-06-28" version="1">MKEVLCPKCGIRMEFMAETESNSEGREVRYFYRCPACGTKINESIMKILKKDSAVIIKVLQ</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    <reference key="2">
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_2_2"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15210" checksum="334BDE832B9E684C" modified="2011-06-28" version="1">MKVFPAQVWRNKESLYSLIAGKCNKCGNLFFPYREICNKCGSTSVSKVKLSGRGTLISYTVSYQTRPGFEKTSPEYVGLIRLDEGIEIVAPLTDITGEIKDNSRVEAVIRRLKADSSNGLIEYGIKFRLVEDEHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A0</accession>
    <name>F4B4A0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvH</name>
        <name evidence="6" type="ordered locus">Ahos_1325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776123.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A0"/>
    <dbReference type="STRING" id="933801.Ahos_1325"/>
    <dbReference type="GeneID" id="10600811"/>
    <dbReference type="GeneID" id="42778381"/>
    <dbReference type="KEGG" id="aho:Ahos_1325"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_2"/>
    <dbReference type="OrthoDB" id="9810at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3 4">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR617453-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="135" mass="15127" checksum="4E51F9F76CFEE963" modified="2011-06-28" version="1">MGEIKVGKYIVLDDRLYTETDEWVKIDGDVAVVGITDYAQKKLRDIVGIELPKVNQEVKAGDPVGTIESVKAASDLYSPLSGVVVEVNEELETSPEIINKDPYGEGWMFKIKIKDKSEAQRLLTPEKYIEKIRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A7</accession>
    <name>F4B4A7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776130.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A7"/>
    <dbReference type="STRING" id="933801.Ahos_1332"/>
    <dbReference type="GeneID" id="10600818"/>
    <dbReference type="KEGG" id="aho:Ahos_1332"/>
    <dbReference type="eggNOG" id="arCOG08458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191161_1_0_2"/>
    <dbReference type="OrthoDB" id="43643at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="82" mass="8833" checksum="7E517FB97ED05164" modified="2011-06-28" version="1">MALSELNNITSQIFGNINIQTVPISEILIVTGIVIASIILFAAFIYGMFKLAKALPSMSVKNFMLLMLVIAAVLIIIGILIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C2</accession>
    <name>F4B4C2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1347</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776145.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C2"/>
    <dbReference type="STRING" id="933801.Ahos_1347"/>
    <dbReference type="GeneID" id="10600833"/>
    <dbReference type="KEGG" id="aho:Ahos_1347"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="32"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="398" mass="45248" checksum="33E3CDC6B12F0AC2" modified="2011-06-28" version="1">MSFSQAIGNLTGESTLLYQEIARRVEKEKGIKTINFGIGQPDVVTFKRIREEAKKALDEGYTAYTPAKGIDELRQKIADFLSQKYGDKIKKDEVIITPGAKTALFLAFLLYINPGDEVILFDPAFYSYAEVVKLLGGKPVYVNINFDKEKGFYIDIDDLNSKITAKTKMIVLNNPHNPTGMVFEPKQIIEIQEIAKERKIILLSDEIYDYFVYEGEMRSVLQDSGWRDYVIYINGFSKTFSMTGWRLGYIVAKKEIIDKMGILAANIYTCPTSFAQKGALAAFDSFDEVREMIKLFKKRRDVMFSELSKIRGIEVYKSPGAFYMFPNMKEVVKKVGSSKELAIKLIEEAGVITIPGEVFPLNVGKNFLRFSFAIDEDKIKEGVKRIKDVIEKIVDEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J6</accession>
    <name>F4B4J6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775002.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J6"/>
    <dbReference type="STRING" id="933801.Ahos_0193"/>
    <dbReference type="GeneID" id="10599633"/>
    <dbReference type="KEGG" id="aho:Ahos_0193"/>
    <dbReference type="eggNOG" id="arCOG07265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840676_0_0_2"/>
    <dbReference type="OrthoDB" id="374329at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021578">
        <property type="entry name" value="STK_08120-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11485">
        <property type="entry name" value="STK_08120-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16226" checksum="30CF6B89F7F0FC52" modified="2011-06-28" version="1">MKLTSEIKNEHGTSVLTILSDPFFLFPAMLKATNVEKIDNKYKVEIPIKGVFYLPFNLITYVTRYISINSVNYVVNIADFAEHRWGNIKIDVEDKKVKLDLNIPLPLDSINSKILGKRIKEFEKNFNELIRVERIKRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K0</accession>
    <name>F4B4K0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0197</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775006.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K0"/>
    <dbReference type="STRING" id="933801.Ahos_0197"/>
    <dbReference type="GeneID" id="10599637"/>
    <dbReference type="KEGG" id="aho:Ahos_0197"/>
    <dbReference type="eggNOG" id="arCOG03462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="495"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="534" mass="59476" checksum="07D7FF27FB201E73" modified="2011-06-28" version="1">MAKEVFLRQSSGLVRDVSPWPSMLANWALITGGTPLLIISWLWLAPGANWALAYLITLIPTLGMAFLYYVAAASMPRAGADYVFNSRAIHPAVGFANYWGLFIAFVLSQGFYSYLGASWLGYLFTGLGLYYHSSYLLSIGSFFSSKLGRIIWGLLVGTLITSAIAFSTRFHWRFVLITGIISVIANIIMFIALLEINPSVFSSVLSSFSGISNATQSVISCAESNGLKFYPVIYATLLASPAVWYYYSWYNVPASWSGEMKKVRLNVFLSIVVAIILSAVYYILFTQLNLDSFGAKFLTSYSFLYYKKCCCGLNITNPLICNLSSIGAFTPFFALLVLGNPILYIIMFIAIWLPNYYSGPPLVVGLSRYLFSWSFDRIMPSWMADVDERFNAPLKSIILITILSAIGVIMYAYLPILSLVDVTVAFEISYAIFALSVALMPFLRRELFETAVIVKRRILGIPVISLIGFSTFSFLVYITYITWGNPVLLPVNLPTILSLVLMYGSGFAIYYIAKLINLRKGVDISLVFKEIPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N1</accession>
    <name>F4B4N1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N1"/>
    <dbReference type="STRING" id="933801.Ahos_1378"/>
    <dbReference type="KEGG" id="aho:Ahos_1378"/>
    <dbReference type="eggNOG" id="arCOG05983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194984_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="81" mass="9123" checksum="7E23945E61488069" modified="2011-06-28" version="1">MVLEGLIFNSLLLNSIFMKIADILIVEDSSKKVNENKIKEVLDKINVEKIDKININRIHIPGLSDDDILGVHVIVRDVAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P2</accession>
    <name>F4B4P2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF61 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P2"/>
    <dbReference type="STRING" id="933801.Ahos_1389"/>
    <dbReference type="GeneID" id="10600878"/>
    <dbReference type="KEGG" id="aho:Ahos_1389"/>
    <dbReference type="eggNOG" id="arCOG01921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850723_0_0_2"/>
    <dbReference type="OrthoDB" id="15458at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15952" checksum="4A64077C8859B796" modified="2011-06-28" version="1">MLDKILELGLKEIFGSSPAEKVTLKDALQGKNRIKLNNGFYHELNLAEVKDFSSKVPLYLWSLVYLPIIILKLPEPGQFSLEGSEWDKKAVSLILNKEEGDKIILNTAEVEDLLRRYKTLIFITLSSNILFSANSELNEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P7</accession>
    <name>F4B4P7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">H/ACA RNA-protein complex component Gar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776192.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P7"/>
    <dbReference type="STRING" id="933801.Ahos_1394"/>
    <dbReference type="GeneID" id="10600883"/>
    <dbReference type="KEGG" id="aho:Ahos_1394"/>
    <dbReference type="eggNOG" id="arCOG02466">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186525_0_0_2"/>
    <dbReference type="OrthoDB" id="60264at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="90" mass="10751" checksum="FB7A59E5A3E3C879" modified="2011-06-28" version="1">MRKVRIIELGHFYRYTLKNKWLIKIENKNINFIKNDPIGFVVLDENKQRVGKVLDIIGNVNSPYALVEPFPHYNPPKEVYIELVEKVRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W7</accession>
    <name>F4B4W7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UbiE/COQ5 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775049.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W7"/>
    <dbReference type="STRING" id="933801.Ahos_0240"/>
    <dbReference type="GeneID" id="10599680"/>
    <dbReference type="KEGG" id="aho:Ahos_0240"/>
    <dbReference type="eggNOG" id="arCOG04348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_12_0_2"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004452">
        <property type="entry name" value="PRK05785.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="243" mass="27842" checksum="374F468C7EC17DA5" modified="2011-06-28" version="1">MQKIRVTNEDLRNIYNDIPRFYDRANAMISFFQDIKWRAELIKTVQYYCSDPKIILDVASGKGELSYVASKLVKSRIVMVDYAENMLKSSLIEGDKVLASFYNLPFRDNVFDAVVSSFALHAADDIGAVVKEMTRVSRRVVGVIAMGKSDNWLLRNYVAFYLRFIQPYLAALVGAKPLDYKYIYYIYRKIPTNSQLKSIISKIFDLKVFKVKALGSVYIFVGLKKGTEGKECEKSIKNEEKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X8</accession>
    <name>F4B4X8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4X8"/>
    <dbReference type="STRING" id="933801.Ahos_0252"/>
    <dbReference type="KEGG" id="aho:Ahos_0252"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="114" mass="13259" checksum="13139521E3384FC4" modified="2011-06-28" version="1">MNEADNLLSKGDLVQACEKYYKAAEEAIKLLVIENNLKEIIKEVENKGRWESESLFKASKLLRNKYPEIAIQWRNAWTLHVEGFHEISLNEKEVTKLKEDVRKLVVIAVVSSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B503</accession>
    <name>F4B503_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SRPBCC family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775084.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B503"/>
    <dbReference type="STRING" id="933801.Ahos_0277"/>
    <dbReference type="GeneID" id="10599717"/>
    <dbReference type="KEGG" id="aho:Ahos_0277"/>
    <dbReference type="eggNOG" id="arCOG05400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131895_1_0_2"/>
    <dbReference type="OrthoDB" id="7914at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15675" checksum="46AE27D61F0FFC2B" modified="2011-06-28" version="1">MITFSVSKKFDVSPQAVWNIVKDINSIPRYWKGTRELHVNKVAEGIYEGVVRFAFPSKGRIRLIVDDKNRKLTFNYLSGPIKGYNIVEVKENEIISSWRVKMSLLLALAESWNAKHFKQGTEHALDRIISEAKSGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B527</accession>
    <name>F4B527_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776244.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B527"/>
    <dbReference type="STRING" id="933801.Ahos_1446"/>
    <dbReference type="GeneID" id="10600942"/>
    <dbReference type="KEGG" id="aho:Ahos_1446"/>
    <dbReference type="eggNOG" id="arCOG01790">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084121_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="214" mass="24114" checksum="0ADAD10302E7BB66" modified="2011-06-28" version="1">MTILEVAGGTGIGGIALSKNLLSKGFKVRLIITDLRPKALEKAKEFSRDELGFEAETYVIDAREIHKLGIKADIILMYGNSHPTFSTFDMAKFILSSKLSLNSGGSLIIQGHNMFYDYIITSGYKEILPERVRKDKILLSIHEGYDEYRGMFKRLYIDLISGRKAEIELKYWDFGELIGLCKIFFSNVDLKMVGGHRGFVICSNLIEDVTSILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B541</accession>
    <name>F4B541_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mevalonate kinase</fullName>
            <shortName evidence="11">MK</shortName>
            <shortName evidence="11">MVK</shortName>
            <ecNumber evidence="11">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mvk</name>
        <name evidence="14" type="ordered locus">Ahos_1460</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776258.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B541"/>
    <dbReference type="STRING" id="933801.Ahos_1460"/>
    <dbReference type="GeneID" id="10600956"/>
    <dbReference type="KEGG" id="aho:Ahos_1460"/>
    <dbReference type="eggNOG" id="arCOG01028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_2"/>
    <dbReference type="OrthoDB" id="19001at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="12">
        <location>
            <begin position="75"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="13">
        <location>
            <begin position="226"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="318" mass="34593" checksum="1006FAFBCC66393F" modified="2011-06-28" version="1">MIEVEVPLKLTLFGEHAVVYGEPAIAMAINEKMKIKIVPYHKMILKSNSLSIKGIKVDLEEMKLESEETSKVLSYALSTINFFEKEYGTRKNALIEIESPVDPSVGLGTSAAVIVGIVGGYSRYLGYELTREEIAKISHKIELTVQGLGSRMDTYTTSLGGLIYFHKGGGYEKIRGEIRITAGYIRRIATTAEILKRVKSLKEKNPDLFNDLISSIGKTVNKAKIAIEKGDEEEIGELMYVNHGLLMSLGVTLPPIDNLVSTAKILGLKGCKISGGGGGGSTICLEDERARILLSTIGAKIIDSGISQEGVMIKEINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A3</accession>
    <name>F4B5A3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0306</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="AEE93197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775113.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0306"/>
    <dbReference type="GeneID" id="10599747"/>
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    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="295" mass="32957" checksum="0CB4125206458A0E" modified="2011-06-28" version="1">MIRLNNVTKALNHKNIIDNVSFSVNNSEIVGYVGPNGAGKTTTMKIIVGVSNPSSGDVIIDDYSITKDKDKAQRNIGWIPEIINFDENEKAIDYFYYIASFYGIGKEEAKRIAEELFHLVGLEGKENQKIKTFSQGMKRRYAIALGLINNPKNLILDEPQNGLDPQGIILVRELLMKMKNKGCAILFSSHLLGEIEKIADRIVFIDKGKIIKEVKAKELSGVVKIVLKEVNQGILTKIMEIGDARVKGNVIYLNEDVDLIEVIQAVGKRNIIELKQSYDLEDIFGSLYGLNNSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A5</accession>
    <name>F4B5A5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein, ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0308</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5A5"/>
    <dbReference type="STRING" id="933801.Ahos_0308"/>
    <dbReference type="KEGG" id="aho:Ahos_0308"/>
    <dbReference type="eggNOG" id="arCOG00732">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142189_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
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        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37318">
        <property type="entry name" value="BSL7504 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37318:SF1">
        <property type="entry name" value="BSL7504 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13601">
        <property type="entry name" value="HTH_34"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_MARR"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="11986" checksum="7779210A07148A65" modified="2011-06-28" version="1">MEILADPLFSSPIRIGILLALAGINKITFSELQKFLNINKSTLSVNLKILENYGLVEIKTKIFNDRPREVIIITKKGKEFVEKYLEAISKYKEMIENQDKNDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5F4</accession>
    <name>F4B5F4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776293.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5F4"/>
    <dbReference type="STRING" id="933801.Ahos_1495"/>
    <dbReference type="GeneID" id="10600995"/>
    <dbReference type="KEGG" id="aho:Ahos_1495"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="OrthoDB" id="39591at2157"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="15"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="108" mass="12399" checksum="824278026F937FC0" modified="2011-06-28" version="1">MEEDLKNKKESIKCCYRLSETDVECLFKLIELNKPITSIELAQIMGFSKTTAETSLKRLMDVGLVNRIKIEEKKIGRPKFVYSVINNIWEKISEDLKQCAKKILSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H1</accession>
    <name>F4B5H1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Conserved transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1512</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H1"/>
    <dbReference type="STRING" id="933801.Ahos_1512"/>
    <dbReference type="GeneID" id="10601012"/>
    <dbReference type="KEGG" id="aho:Ahos_1512"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
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        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF18">
        <property type="entry name" value="S-ADENOSYLMETHIONINE_S-ADENOSYLHOMOCYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="3"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="135"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="275" mass="30206" checksum="FE61605BD2754261" modified="2011-06-28" version="1">MIRGYLTILGVIIVWGISFPLSKIALYFMSPFVLTFIRFIIGGLILTAYGKGIIYGKKEAINAILNMGIFVILLNLAINYSTNPALASVLIYMQPIFVALFSRLRGEEILFPQLIGIIVSFTGIFISVDSVNFSLGSLIAIVAAILWAVGTLYYSNLKNRDIVKLNAFMSLFSSLFVIPILPFDFYFKPSALGIGVGILVAIIAQALGFIFWFSSVKDLGATTASSIVILVPVSAYLFSFIILDQIPNIFQIFGSSLALIGVFISQYSRVYLSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H5</accession>
    <name>F4B5H5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamine amidotransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1516</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776314.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H5"/>
    <dbReference type="STRING" id="933801.Ahos_1516"/>
    <dbReference type="GeneID" id="10601016"/>
    <dbReference type="KEGG" id="aho:Ahos_1516"/>
    <dbReference type="eggNOG" id="arCOG03639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086262_0_0_2"/>
    <dbReference type="OrthoDB" id="350529at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="228" mass="26374" checksum="2E51F6A6A3AA39A6" modified="2011-06-28" version="1">MCRMFAYVGDSKEELTKLYEALKESAKNDVIASKYGLNPVHGDGWGYVIYDGERIYFYKSKNPIFTESLVLPSIEGKFYAIFHARQATDKSTVSARFAHPFYGDDENYFYFFAHNGSVDKEKLAHDLNFQGQTTIDSELALKFLIKNGIEKGIDLLMKEYTKSALNVFILRISRSDGSAELYYVNYYTRKDRSEYYKFYRTENAIFSSTLTYFGIRGVEVEEGKLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5U0</accession>
    <name>F4B5U0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0417</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">The Mn(2+) ion enhances activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775221.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5U0"/>
    <dbReference type="MEROPS" id="M20.008"/>
    <dbReference type="GeneID" id="10599857"/>
    <dbReference type="KEGG" id="aho:Ahos_0417"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_2"/>
    <dbReference type="OrthoDB" id="247417at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08021">
        <property type="entry name" value="M20_Acy1_YhaA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000014">
        <property type="entry name" value="N-acyl-L-amino acid amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053493">
        <property type="entry name" value="Thermostable_CP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040868">
        <property type="entry name" value="carboxypep_CpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF63">
        <property type="entry name" value="METALLOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G09600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="191"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="394" mass="43064" checksum="A2A821675DE2659C" modified="2011-06-28" version="1">MDIEKLKKDVLEIEDKIIEIRRKIHENPELSYKEYNTAKLVAETLKSLGIEVKVGVGLPTAVLGILKTSKPGKVVALRADMDALPVEEMTDLPFKSKIKGVMHACGHDTHVAMLLGGAMLLAKNIDMLSGEVRFIFQPAEEDGGLGGAKPMIDAGVMDGVDYVFGLHISSAYPAGVFATRKGPLMATPDAFKITVHGKGGHGSAPHETIDPIYISLLIANAIYGITARQIDPVQPFIISITSIHSGTKDNIIPDDAVMEGTIRSLDENVRKKALDYMERIVSSICGIYGAECKVEFMKDVYPITVNDPETTEEVMRILNNISKVEETQPILGAEDFSRFLQKAKGTYFFLGTRNEKLGCIYPNHSSKFCVDESVLKLGALAHAALSIEFTNKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5U7</accession>
    <name>F4B5U7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5U7"/>
    <dbReference type="KEGG" id="aho:Ahos_0424"/>
    <dbReference type="HOGENOM" id="CLU_2534476_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="83" mass="9602" checksum="ABAD2159BB2DF9A3" modified="2011-06-28" version="1">MGLFFNLLFKRVPINKPFPSSAPVFHQSSRVVPFILTTVIRGSLKLFIFKIKAFLKCLFLQLINDYFTITSNLKIHKLVLDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5X9</accession>
    <name>F4B5X9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide biosynthesis-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776392.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5X9"/>
    <dbReference type="STRING" id="933801.Ahos_1596"/>
    <dbReference type="GeneID" id="10601097"/>
    <dbReference type="KEGG" id="aho:Ahos_1596"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046380_0_0_2"/>
    <dbReference type="OrthoDB" id="34743at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052556">
        <property type="entry name" value="PolySynth_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424:SF1">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13440">
        <property type="entry name" value="Polysacc_synt_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="459" mass="51254" checksum="F0191FC19257C7F6" modified="2011-06-28" version="1">MNPLSGALKFLSTTLFNSVFALAFFLVAGHFATPSFIGKVAIIQLMETITRAFFSILPYQLVTREVSHSYASSQGYNRLVYTSLSYSLLVSPLLLFLLFFPSYLWMSIPYFVLYLFTNYQDQLLIGLGKFTEVNVGNAVFTISRWGFSIIAIFYHSVELLILIWTLGAVMKAVYYQIYLPFKFSLDKATFKEIAKVGFPIYLTGVVSFISGQGDRVVTAFLLGSYYLGIYQLVALAAVVPSMLISSFSSSLLPSSTYYYTKGKDIKEISSVSFRIVTLISLPLAILGYAISPLFISKFFPQYVLGIPSMQLLILSLTSTMPLQLLSTFMIAAKKNYRPFIIIGIISAAEVVGVSYYLIPRIGIYGAAIAQAFNAIITSLLYLLFSHFQGVFKLERREIVGLALIGLSFLAFINWEVVLIVVLLGSKLLGIISNEEVKVIEGFTPYSLKRIIRILYLIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B633</accession>
    <name>F4B633_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Quinol oxidase (SoxABC), cytochrome B subunit, soxC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_1653</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776446.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B633"/>
    <dbReference type="STRING" id="933801.Ahos_1653"/>
    <dbReference type="GeneID" id="10601153"/>
    <dbReference type="KEGG" id="aho:Ahos_1653"/>
    <dbReference type="eggNOG" id="arCOG01721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038033_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.810.10:FF:000012">
        <property type="entry name" value="Cytochrome b/b6 domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="entry name" value="Cytochrome Bc1 Complex, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005798">
        <property type="entry name" value="Cyt_b/b6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053595">
        <property type="entry name" value="Cytochrome_b-type_ET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041076">
        <property type="entry name" value="cyt_b_SoxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19271:SF16">
        <property type="entry name" value="CYTOCHROME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00032">
        <property type="entry name" value="Cytochrom_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13631">
        <property type="entry name" value="Cytochrom_B_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="Transmembrane di-heme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 N-terminal region profile" evidence="11">
        <location>
            <begin position="7"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b/b6 C-terminal region profile" evidence="12">
        <location>
            <begin position="217"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="553" mass="62537" checksum="3B8323DC66A8D6D7" modified="2011-06-28" version="1">MSISKRVSNWFNERLKLDELPFFRTPDYMYHVSDWLGALVAAAFMYTVISGLILLLYYDPDNGYNSTQFIINQVPYGSVVLYSHLYGAYAMIILAYIHMFRNYFVGAYKKPRELLWILGVFLLVLTMGASFFGYSLIGDVLATSAVDVGAGIIESIPGMQWLVPFFFGNYDNGQYGRVLAWHIIFVALIGLLFVFHFFLAESYGMMPSRKVKSKAPAVYTREEWMKFNPWWPRNFVYMLSLILMTWGFILIIPNALAYLNGLPQYYNPFLNPKPAPPPTSPLAATVTTYPPWFFLFFYKIADFSSNVLLDLSIGAIIPLVYLILLPFLDRSEELHPLSRKIFVGIGILMIIYLIQTTLWGDIAPGVPVAFKCQVVALLPPAVIIAIGLWILPSKKSSEEPRVKHSFLHLGKALAPLAILGFVIISLLFVGGLAEFVNYPSPITLVILLPIASLFVIGAKRIAPIILRMEQQQTSSTPSPEVSKLEMKKKFAQYIIVVLFVLSLVLMVTLWTIPPTGYESNMFGVDLGLIFVMWGEAVSLYHYIVYKKPNESYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B6</accession>
    <name>F4B6B6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Twin-arginine translocation protein, TatA/E family subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1658</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776451.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B6"/>
    <dbReference type="STRING" id="933801.Ahos_1658"/>
    <dbReference type="GeneID" id="10601158"/>
    <dbReference type="KEGG" id="aho:Ahos_1658"/>
    <dbReference type="eggNOG" id="arCOG02694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="10942" checksum="7FA504E1FEDF4615" modified="2011-06-28" version="1">MFALQPSDIAIIIIVALVLFFGTSKIPELFRSMGKAVGEFKKGRLEAELEMQQMQQQNQPQVQNQADKAAELERQIQELQKQLEQLKSQKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E3</accession>
    <name>F4B6E3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvH</name>
        <name evidence="5" type="ordered locus">Ahos_1686</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E3"/>
    <dbReference type="STRING" id="933801.Ahos_1686"/>
    <dbReference type="KEGG" id="aho:Ahos_1686"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002270">
        <property type="entry name" value="PRK01202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="4">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="151" mass="16774" checksum="D7A56C7A404612CB" modified="2011-06-28" version="1">MVDLVVESNCEIPENLLYYIEGKNTVWAKQESADVILVGITDIAQTMAGKIVKVRIKKKGIKVERGRPLATMESGKWAGPVPAPVSGEIIDVNAEAEKDPVIINRDPYGKGWLVRMKVTNPEELKQLYTGEQAIQKLKELIASEKISCKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G8</accession>
    <name>F4B6G8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mercuric reductase</fullName>
            <ecNumber evidence="4">1.16.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Hg(II) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">Ahos_1711</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Hg + NADP(+) + H(+) = Hg(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23856"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16170"/>
            <dbReference type="ChEBI" id="CHEBI:16793"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.1" evidence="4"/>
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    <sequence length="435" mass="47741" checksum="3E679254D84F3517" modified="2011-06-28" version="1">MKDLVIIGYGAAGFASLIEANELGIKPVLIGYGPIGGTCVNVGCVPSKRLLHLGEKGRDFFSSFEDTKQFVNRSRKEKYEDVLSYYDVELIEGKAHFISPHEVKVGDKVIEGKKFIIATGSSPFIPEIPGLKDLGYWTNVEALNPDRKISSLVIIGGRAEALEFSQMYRNFGVEVAILQRSKTLIPDWEPEISLEIQKVLEEKGIYVVTDVKVKEVKKGEGGKVVVTNKGEVEAEEILMATGRKPNVDLNLSSAGVELNEKGGIKVNDELQTTNPNIYAAGDVIGDKMLEALAGYEGTVAVRNAIKGEHKKIDFLSVPQVIFTRPNLARVGLNSFDGDFDSRTVKMKDIVKAQIIDEEKGLIKMVVEKGSKRIMGVHVMAENAAEFIGEAALAIKHRMTIDDIIDTVHVFPTVAESLRIVALAFYKDVKKLSCCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
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            <begin position="7"/>
            <end position="24"/>
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        <location>
            <begin position="30"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="10714" checksum="3616C83BD734AB8E" modified="2011-06-28" version="1">MKKSDLFLVSYSFSSIGGPLALVAQFSRGLSPLDVILSSILFLPTVYVTKKIKGDKGLYEYLLNRVKFLRLSSISDSFPLFKLYCGLRCLLYFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I2</accession>
    <name>F4B6I2_ACIHW</name>
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        <dbReference type="NCBI Taxonomy" id="933801"/>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
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        <property type="entry name" value="APC_superfamily"/>
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    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="14"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="430" mass="46230" checksum="715833178F25E25B" modified="2011-06-28" version="1">MKRGEKEVSLAEATAIGLGAIIGAGIFVLSGTSIALAGSYSLLAFIFIGVLSVLTALILGELATILPHVKGSTYSYAFEAFGHELGMLTGVMVYFSYSTSISAIAEGFGSYLSSLVGMHLVIPFAILLIGVLTAVNLLGIQKAAQADLILVLLKLSVLSFFIAFAIIIALPHFTPYHFYSTPQQSTVTNFFSASIAIFFAYTGFQVITTFADKVRGGPRVAARAVMISVVVSMVFYVLVVLSMIILVPASEFKIVADPLEFALSYAKAPSWVIYVIDAGGLIATTSATLAMILASGRTLYQMSLDLGLPSFLKKYDEERDVPTNTVILSSVIAVVTLFSGNIFVIASISNFGLLFSFVVSALALVHYRRKGVVGSYKTPFYPYSVALTVVLLLSLLLGFPREALEINIGIMIMIIFASYVAKDIREERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I3</accession>
    <name>F4B6I3_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">PaREP1 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776518.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6I3"/>
    <dbReference type="GeneID" id="10601226"/>
    <dbReference type="KEGG" id="aho:Ahos_1726"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_0_1_2"/>
    <dbReference type="OrthoDB" id="39997at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="163" mass="18629" checksum="E4D5F2CF5B985025" modified="2011-06-28" version="1">MEELIKKAEEKGIDVEDLIISAMSKEDPQGGIKLRLELAKKYMAEAEEYLKKGDAVQASEKAYKVAEEIVKALSEKFNLQEYQQAVKEGRWYTLGSAVSSLSKKLGNWIVNGWSSAYFLYVWGFHESKLSTDDIIPYLDEVKRMLEEAEKVLNAYESMHTTFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K7</accession>
    <name>F4B6K7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775333.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K7"/>
    <dbReference type="STRING" id="933801.Ahos_0529"/>
    <dbReference type="GeneID" id="10599970"/>
    <dbReference type="KEGG" id="aho:Ahos_0529"/>
    <dbReference type="eggNOG" id="arCOG03142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431674_0_0_2"/>
    <dbReference type="OrthoDB" id="34658at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="184" mass="20988" checksum="06CE91903C7A2616" modified="2011-06-28" version="1">MGIKKVAIKVNNPRIFRQLVQELRKSDLIPTVDSGDLTISDFDKNADVFIQGEDEIKKAITYLQCLKLGKRKFDELLIGIDTNSPKLTVVILGDGIIIDTLEAWIDEIEDIIEEVISKYPYKRIYIGVGTGNKYGELVYKLLSIRFPFVKKVNESRTSLRNPYVNIKDKDVRAAYMIALRSTKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P2</accession>
    <name>F4B6P2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0564</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775368.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6P2"/>
    <dbReference type="STRING" id="933801.Ahos_0564"/>
    <dbReference type="GeneID" id="10600007"/>
    <dbReference type="KEGG" id="aho:Ahos_0564"/>
    <dbReference type="eggNOG" id="arCOG01238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_498421_0_0_2"/>
    <dbReference type="OrthoDB" id="33297at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00919">
        <property type="entry name" value="Heme_Cu_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="563"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="2">
        <location>
            <begin position="20"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="587" mass="65020" checksum="F0E43BC47DBF0D80" modified="2011-06-28" version="1">MSLKDLVVSLFQLDKDWTTRIVMAMLVMGVIWGLLGVIDSLMVRIQESTWGTYGVLAMTSQEYYAGITLHAERDLFGFAEQVEFALFIYFTIKLLNLQPRAKWLLNIAFIAINISMMFMEGPIVAFPTFNDNYFSATDWYYISPMGIPPYSEYVVSPLFFIGWLLLDAFTYMASVWIIYHYYIASKSLKEKLPVALVFFLMNTLLYAIGYSGVTAADIWDILAYAGIVGLNPIANQIAFWIFGHAIVYMAWLPAVAALYLLIPTLANKPLFSDRMARVSALLYLIFSNNVPVHHLYMVNLPVAIKVLQEILTYAVVVPSMMTFLNLWATAKGAQVNFNVITAFVATSFAGAIAAGVTGIANATIAFDSIVHNSMWVVGHFHAMILLSIVPAAMAVLYFMIPMMTGRQWYSSKMAWIHYIGYTIGASILIIGFEMIGFYGIVRRAEIYPRVPGLVFAENLATAGALIAEIATLVWFVNLVATLVKGRTARLEGLSLGQLINTVAMSLDWSSAGFKLNNFNKIGKTMNSKALGAYWALGIIGALIIVISTIPLALGGDMYNAMPWAWIILLTLGIILISYPVLKGAKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S1</accession>
    <name>F4B6S1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776529.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S1"/>
    <dbReference type="GeneID" id="10601237"/>
    <dbReference type="KEGG" id="aho:Ahos_1737"/>
    <dbReference type="eggNOG" id="arCOG00729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_5_0_2"/>
    <dbReference type="OrthoDB" id="168412at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="14701" checksum="2E0AFB0F882307B0" modified="2011-06-28" version="1">MKGEFLLDSSALYPLLNYADKVDVSKIYILSLTFYEVGNAIWKEFYIHKRIKDPFTLSLLFQKFLHKLKVLSDPPANEVMKVAVDKGLTFYDASYVYSAEANSLTLVSEDKELIKKANAIPLKEFIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z5</accession>
    <name>F4B6Z5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPP transmembrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1813</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776603.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z5"/>
    <dbReference type="STRING" id="933801.Ahos_1813"/>
    <dbReference type="GeneID" id="10601313"/>
    <dbReference type="KEGG" id="aho:Ahos_1813"/>
    <dbReference type="eggNOG" id="arCOG12781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736493_0_0_2"/>
    <dbReference type="OrthoDB" id="40881at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058581">
        <property type="entry name" value="TM_HPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="TM_HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="HPP transmembrane region" evidence="2">
        <location>
            <begin position="9"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="148" mass="16211" checksum="E47C9501320A2838" modified="2011-06-28" version="1">MGLGRKYKLFAFLNLAISITIVTFASIETHIAFILPPFLATSATKLPDLAWKFQRSLVIVSSYLLSASIGIIFVLLGSGIFIAVLASIIAYDIELILNIEHPPSILATFLGVLKRVSPIYILHPVLVGVLTIEGINYVISRIMKISKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B712</accession>
    <name>F4B712_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B712"/>
    <dbReference type="STRING" id="933801.Ahos_0607"/>
    <dbReference type="KEGG" id="aho:Ahos_0607"/>
    <dbReference type="eggNOG" id="arCOG08481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193463_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="99" mass="11115" checksum="F4C2CDB5A7DAE3BB" modified="2011-06-28" version="1">MEFFLGNFIAIFLHFRNVDVEDKILLVRGILGSIAGVISAFSSSFIYAAITVLVSYIISIPIVVFYFKIKRNWLVFGKGSLTLAIAWFLILVSVYNVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F4B736</accession>
    <name>F4B736_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="9">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="9">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="9">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aspS</name>
        <name evidence="11" type="ordered locus">Ahos_0632</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775435.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B736"/>
    <dbReference type="STRING" id="933801.Ahos_0632"/>
    <dbReference type="GeneID" id="10600079"/>
    <dbReference type="KEGG" id="aho:Ahos_0632"/>
    <dbReference type="eggNOG" id="arCOG00406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04316">
        <property type="entry name" value="ND_PkAspRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="135"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="9">
        <location>
            <begin position="190"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="211"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="401"/>
            <end position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="9">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="430" mass="49090" checksum="26FDB95889400ECA" modified="2011-06-28" version="1">MLKSDFVSEISPEQDGKEVTVAGWVHTIRDLGGKKFILLRDRSGLGQIVIDKSNTKAFELSKDLTQESVILVKGVVKADKRAPNGVEIHANELEILSKAKAPLPLDVSGKVNADLDTRLRERLLDLRRLEMEAVIKIQSEAIRAFREYLYSQKFVEIFTPKIIATATEGGAQLFPVIYFGKEAFLAQSPQLYKELLAGTIERVFEVAPAWRAEESDTPYHLSEFLSADIEVAFADYNDIMNIVENTLDFMVNRVRSNCQKELKILNHTLPEIKKPIKRITYSEAIEILQSAGVNIKFGDDIGTPEQRILHQKLNEDLYFIIDWPSTARPFYTRRKSNNPELSESFDLIYRWVEIASGSSRNYKREVLEEELKTRGLNIASFEFFLKWFDYGMPPHAGVGIGVQRLMMLFTGLQNVKEISLFPRDKKRLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B744</accession>
    <name>F4B744_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0640</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775443.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B744"/>
    <dbReference type="STRING" id="933801.Ahos_0640"/>
    <dbReference type="GeneID" id="10600088"/>
    <dbReference type="KEGG" id="aho:Ahos_0640"/>
    <dbReference type="eggNOG" id="arCOG00980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073526_1_0_2"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040825">
        <property type="entry name" value="FKBP26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054016">
        <property type="entry name" value="FKBP26_IF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048261">
        <property type="entry name" value="SlpA/SlyD-like_ins_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF3">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18046">
        <property type="entry name" value="FKBP26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22199">
        <property type="entry name" value="FKBP26_IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="231" mass="26599" checksum="8445D30ACADFA44A" modified="2011-06-28" version="1">MFKDNDFVYIDYVSKIKDTGEVIDTTIEEEAKKANIYDPEKKYKPLLVIIGEHRVLEGLEEALRNFNEGEQKEIEIPPSKAYGERDPSKVKLVSLGELKRQGINPKPNMVIRLAEGGYATVKSVSGGRVILDLNHPYAGKTIIYNVKVVKVLKDEKEKIQALVERWFGENNKMTFELTEDKKSVKFEVPKDYFLLEDLQTRKYVLAKDIITFVLPESVVSFVENYNKETFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B752</accession>
    <name>F4B752_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="6" type="ordered locus">Ahos_0648</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B752"/>
    <dbReference type="STRING" id="933801.Ahos_0648"/>
    <dbReference type="KEGG" id="aho:Ahos_0648"/>
    <dbReference type="eggNOG" id="arCOG01704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_2"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="5">
        <location>
            <begin position="192"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="197"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="410" mass="46001" checksum="60D1035D73CBC279" modified="2011-06-28" version="1">MTHPSKRIIGEISDELKGKKVLLGVSASVSLYKSLDLARDLMRRGAEIKVMMTPSASKLISPEMFTWATGNKTVVKIGGKLEHVQLAEEFDVMIIAPSTMNIVTKLAYGITDNNLVLTAINFLQLNKKVILVPAMHLQMWNSPQLQSAIEILRKNNNVEIIEPKLVNNLAHYPDIEYLTSRITSYILREKDLKGLKIVVTAGPTREYMDPVRFITNPSSGTMGVSIANEAYFRGADVVLVHGPISSHLHPYSPTIEVETTEDMLKNVVNLVEKEKFNIVILAGAPADYKFKVIRGEKIDSHTEVPKVELEKTPKISEYIRGKAFVVGFSAETVNNDDELIEKAKIKKERHGFNMIVANNVKRKDIGFSSEYNEVIVITNSEIIKIPKSYKTIIARKLLDIVREEFIKSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B763</accession>
    <name>F4B763_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl13</name>
        <name evidence="6" type="ordered locus">Ahos_0659</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775462.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B763"/>
    <dbReference type="STRING" id="933801.Ahos_0659"/>
    <dbReference type="GeneID" id="10600110"/>
    <dbReference type="KEGG" id="aho:Ahos_0659"/>
    <dbReference type="eggNOG" id="arCOG04242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="OrthoDB" id="7668at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005004">
        <property type="entry name" value="PRK06394.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="151" mass="16960" checksum="AA9B66CD5A326990" modified="2011-06-28" version="1">MSEVVIVNAENQILGRMATNVVKLLKEGKKVVIVNAEKAVISGPKSRVIAGYKLYFSLRTLFNPEKQGVRRPRSPINIVKRTIRGMLPKTPKGRQMLKSVKVYIGVPQQYNGKNFISFPDADVKRLKGKYITVGELAKEMGWKVYVRATGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B770</accession>
    <name>F4B770_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeal serine protease family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776605.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B770"/>
    <dbReference type="STRING" id="933801.Ahos_1815"/>
    <dbReference type="GeneID" id="10601315"/>
    <dbReference type="KEGG" id="aho:Ahos_1815"/>
    <dbReference type="eggNOG" id="arCOG01937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523407_0_0_2"/>
    <dbReference type="OrthoDB" id="40661at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="522" mass="57979" checksum="514584132FF024AE" modified="2011-06-28" version="1">MNLKNQFVYILGVLILASIVAQIYIACRHDNEEMKVIVINNGTGYIGDPPASLGNSEDYLINPVFPAHTIASQYAYVTYGNGRVFIGGFYLSGNGYLAGAKGAAVVSSMLAYQPLTSYDYYLYITAPSCINVAGAVKTDSATVMFLASMLGQESSLNKSMYFLGDVGLEGYMYSTGVVYQRIVQLYNGGIYYLVVPKMMALAEGKNYQMAQEFALQHHEVLLTVCSIPQMYQMETSEPLPQPIGYLDLQYNASAGYHYLCSLYKELNSSVSNQTVKEEALSCWLKAVGLAEEGDYYAFYFYPNPAINAIALLYSQDSSFNIDEEVCKAVNCAGNNLWKIETAAEAAYLAQGNISDKILADAWAALSLSITAGPTITPLGLYEGVYNAYVIDVYAAEYYDAVIGSKAGNLTLLTDVFDNHNLIYDYGFFANYYAKLAIDFRNYFLDQLNSTALQEVETSICNSMIHEDQILENAAAYYDGPSVVGYIMMSYGMENHNLCYLYYAMPFIRMTMNMEQLTWYNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B796</accession>
    <name>F4B796_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar fermentation stimulation protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sfsA</name>
        <name evidence="4" type="ordered locus">Ahos_1843</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SfsA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B796"/>
    <dbReference type="STRING" id="933801.Ahos_1843"/>
    <dbReference type="KEGG" id="aho:Ahos_1843"/>
    <dbReference type="eggNOG" id="arCOG04115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052299_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22357">
        <property type="entry name" value="SfsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00095">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005224">
        <property type="entry name" value="SfsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040452">
        <property type="entry name" value="SfsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041465">
        <property type="entry name" value="SfsA_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00230">
        <property type="entry name" value="sfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545:SF2">
        <property type="entry name" value="SUGAR FERMENTATION STIMULATION PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03749">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17746">
        <property type="entry name" value="SfsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SfsA N-terminal OB" evidence="3">
        <location>
            <begin position="41"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar fermentation stimulation protein C-terminal" evidence="2">
        <location>
            <begin position="116"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="240" mass="27741" checksum="3F5D7783D9310720" modified="2011-06-28" version="1">MRRKYNWNSSPQIIKSTFCIFFNMLVYEFKETLKEDIVEARLNRFIVKTKSGRICHLHDPGRLKELIYPGNKILIRETSEKNNRKTPCQVTAAWDGRWVITDSSIHNVIAKKFLPENSRSEVKLGESRIDFFFDNTYVEVKGCTLIKNGVALFPDAPTERGRRHLEELMKAMESGMKAKLLVLVMRDDASCFSPNAETDKKFAETFYLALAKGLEVEIKEFRLEGNKVYYVRDIPLCGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F9</accession>
    <name>F4B7F9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="7">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lysS</name>
        <name evidence="10" type="ordered locus">Ahos_0677</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7 8">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 8">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775480.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F9"/>
    <dbReference type="STRING" id="933801.Ahos_0677"/>
    <dbReference type="GeneID" id="10600128"/>
    <dbReference type="KEGG" id="aho:Ahos_0677"/>
    <dbReference type="eggNOG" id="arCOG00408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_2"/>
    <dbReference type="OrthoDB" id="131570at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF15">
        <property type="entry name" value="LYSINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="177"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="494" mass="57641" checksum="507EF1718CFC440A" modified="2011-06-28" version="1">MKWDERRVKIVEELKAKGINPYPQKYEITHTILQIRQIGLNRQDKPHEPFMFDISTAGRVANIRRHGKASFVDIFDEGERLQLYLRINELGDKYEEFFNYIGRGDIIGVKGDLFYTGKGELTLLVKSYELLAKALIEPPDWTKLSTEFRYAHRYVDFLYNDSSRKAMEIRFKTIKEIRDYLNSKGFIEVETPILQPVYGGALAKPFKSKVNYLNEEWYLRISLELYLKRFIVGGFDKVFEIGKVFRNEDIDVTHNPEFTELELYWAYADYNDIMKLTEDMLKTVVSKVLGDNKLKYKVADKEYEIELSQFRKITMFDSLSEALGKDVEKMTDDELKELMKKYGLIPRGNLYVRGLMIEKLFDKLVTPNLIQPTFITDYPIETTPLCKPHRSKPGLVERFELYIAGMELANAYTELNDPILQDKLFKEEQEMMKRGDEEAHPYDKDFVRALSYGMPPTGGLGIGIDRLVMLLTNNQSIKEVIPFPMLSAKLIEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J1</accession>
    <name>F4B7J1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="7">BPG-independent PGAM</shortName>
            <shortName evidence="7">Phosphoglyceromutase</shortName>
            <shortName evidence="7">aPGAM</shortName>
            <ecNumber evidence="7">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">apgM</name>
        <name evidence="9" type="ordered locus">Ahos_0709</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775512.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J1"/>
    <dbReference type="STRING" id="933801.Ahos_0709"/>
    <dbReference type="GeneID" id="10600162"/>
    <dbReference type="KEGG" id="aho:Ahos_0709"/>
    <dbReference type="eggNOG" id="arCOG01696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="OrthoDB" id="52918at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003104">
        <property type="entry name" value="PRK04024.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="8">
        <location>
            <begin position="5"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="415" mass="45485" checksum="1A1E00A3A2A6ECE5" modified="2011-06-28" version="1">MKKYKILLIIADGLGDRQVKSLDNKTPLEVADKPNIKELLKSSLVGLMDPIGPGIVAGSDTSHMAIFGVDPHRYYRGRGALEALGTGAYLTEGDIAFRGNFATVDENMNVTDRRAGRKIDEGEQLVNELNQKIGEIDGVKVTFYKATEHRVAVVLSGKDLSDKISDTDPHEVGRKIKESIPLDNSISARRTAEIVNNLTKRIYDVLSTSIYNKNRIERGEPPANIILLRGASKFVELPKLYNYTGLKGGAVSATALIKGVCKAIGMDVFTPKGATGGIDTNYDAKADKVIELLKDDKYDFVFLHVKATDAASHDGKTEEKVKAIEKMDYIIGKILNNVGREIVIMFTGDHATPVELKEHSGDPVPVLLYVPDNIIPDNVSDFNEREARKGSLKIIGLDVMNLLLNYSNRATKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J2</accession>
    <name>F4B7J2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="6">2.7.1.237</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="6">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3'-dephospho-CoA + GTP = GDP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.7.1.237"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.237" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775513.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J2"/>
    <dbReference type="STRING" id="933801.Ahos_0710"/>
    <dbReference type="GeneID" id="10600164"/>
    <dbReference type="KEGG" id="aho:Ahos_0710"/>
    <dbReference type="eggNOG" id="arCOG04076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120795_0_0_2"/>
    <dbReference type="OrthoDB" id="15447at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732:SF1">
        <property type="entry name" value="GTP-DEPENDENT DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="UPF0218 PROTEIN TK1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="20026" checksum="5F4BB11921806F01" modified="2011-06-28" version="1">MEIRDKYKVDLCFYLPKEVRKELSRPYGILFKSTEKLLKYVDNFERIITVGDVVTQELLKNNRRVFLSIVDGKTKRNIKLHMRFSNYIMVKNEPAIIRLSAMSYIKAALNSSFSSVIYVDGEEDLLVIPATLYGRDGDLVIYGQPNAGAVALEVCEATKWRVNDIFSKFNVKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7K3</accession>
    <name>F4B7K3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="7">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cbiA</name>
        <name evidence="10" type="ordered locus">Ahos_0721</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>cob(II)yrinate + 2 L-glutamine + 2 ATP + 2 H2O = cob(II)yrinate a,c diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and cobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">The a and c carboxylates of cobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7K3"/>
    <dbReference type="STRING" id="933801.Ahos_0721"/>
    <dbReference type="KEGG" id="aho:Ahos_0721"/>
    <dbReference type="eggNOG" id="arCOG00106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_1_1_2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03130">
        <property type="entry name" value="GATase1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA/CobB_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002204">
        <property type="entry name" value="PRK01077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873:SF1">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="7"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="243"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="7">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="434" mass="48528" checksum="573FFF32900F0F96" modified="2011-06-28" version="1">MTHIPRILISSDRSDSGKTLISSGLMRALSKRIKVRPFKAGPDFIDTGYHKIATRGIPSINLDLFIMGKENVINSLIKYSKGYDISIIEGVMGLYDGIGLDYSTYQLSEITKTPIILIINCENIGSTAGAIIKGLKDYGNAKIAGVIFNKISSEGHFNYCKSSVKDTEVLGYIPFSKDVIVPSRHLGLYTTEDYNPEKAINTISKLLEEYVDKDKIMEIANSAEDLPEVNDLEIQDTEKKVAAIAYDAAFNFYYQENIDILKRKFQIKFFSPLNGETVEDPDLIYIGGGYPELYLKELESSITSSWIKKESYKGTKILAECGGLMYTSKYIIGFDGKKYNMSNIFDLGISAKGKLTIGYTELLTLENSILSKKGEKIRGHEFHISFPVEVNEEKFVFKNLRGKGIKDGYDGVKVQESLATYTHFHFASLQSSAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M8</accession>
    <name>F4B7M8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776690.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M8"/>
    <dbReference type="GeneID" id="10601401"/>
    <dbReference type="KEGG" id="aho:Ahos_1902"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929991_0_0_2"/>
    <dbReference type="OrthoDB" id="43013at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="6614" checksum="EF43EDF34DE53A4D" modified="2011-06-28" version="1">MLKSSGYFTIFPLSTVKGEVYKIKCKYPISLADASSIATAKVLGLSTLFRKRSLSLLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N1</accession>
    <name>F4B7N1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776693.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7N1"/>
    <dbReference type="STRING" id="933801.Ahos_1905"/>
    <dbReference type="GeneID" id="10601404"/>
    <dbReference type="KEGG" id="aho:Ahos_1905"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="110" mass="12871" checksum="01C78A9B25014D26" modified="2011-06-28" version="1">MQIENWIKEADDLLSRGDVVQASEKYYKAAEEAIKLLSIRKNLSILRTVENQKRWTSSILFEAAKNLGGEIYRIWHSAWYLHVFGFHEMALDKSDVEKISIRVKKILSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R3</accession>
    <name>F4B7R3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histidine kinase N-terminal 7TM region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R3"/>
    <dbReference type="STRING" id="933801.Ahos_1937"/>
    <dbReference type="TCDB" id="9.B.151.1.1">
        <property type="family name" value="the uncharacterized 7 tms archaeal/bacterial protein (7-abp) family"/>
    </dbReference>
    <dbReference type="GeneID" id="10601436"/>
    <dbReference type="KEGG" id="aho:Ahos_1937"/>
    <dbReference type="eggNOG" id="arCOG06968">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259158_0_0_2"/>
    <dbReference type="OrthoDB" id="43164at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22758" checksum="5D9A8F2BA74CF5EF" modified="2011-06-28" version="1">MNQEEALIFSSISLVLSIALGYLSWRRYENSKSLMWFFWFLGFVFYFIAALEQEIFALGYCNYPLAAIYIFIVAELVLVLSLGSVQQVPEKWKRIYYYYSAFTTAVLIITIVYKPFTIVVDYMPTNFPALPAGASSMVTIPASGLILFLAARALLFKGNKAKMISTILAIVILGFGGMLSASGFVLALYLSQILGMSLFLYGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R9</accession>
    <name>F4B7R9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776731.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R9"/>
    <dbReference type="STRING" id="933801.Ahos_1943"/>
    <dbReference type="GeneID" id="10601442"/>
    <dbReference type="KEGG" id="aho:Ahos_1943"/>
    <dbReference type="eggNOG" id="arCOG03737">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599090_0_0_2"/>
    <dbReference type="OrthoDB" id="34008at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="19692" checksum="87A7AA92C9DCF128" modified="2011-06-28" version="1">MLSEELVISALKELYDGRPVAFSKIKRKLKADGEELSLVLEKLEKEGKIKKIESGSGKSFELTSSNNGDNKFDLLLKEITEIKDEIKKLQEAVLEKKKLSENYFDEIYNEVKDNLGYAHLKDIRIEMGLTKEEFYSKLKRHIEENYELIAGGEEGFVKKGSVYGIIIKRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T8</accession>
    <name>F4B7T8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CelB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T8"/>
    <dbReference type="KEGG" id="aho:Ahos_1962"/>
    <dbReference type="eggNOG" id="arCOG03858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912231_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013319">
        <property type="entry name" value="GH11/12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="132" mass="15086" checksum="A3F2CE98829BC97A" modified="2011-06-28" version="1">MIWLYHNETLSSYFIKEGEVQVPIYVNGSEITCTFTVYILPHTGSSNGWIGAYYLSNKSLEGKVSIPLTFFIKDEFQWIDKIFPTVNESSYYLDGIQVGMEFNDYNGIAELGYTLYCWIMSTAQGVKILDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z3</accession>
    <name>F4B7Z3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0784</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775586.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z3"/>
    <dbReference type="STRING" id="933801.Ahos_0784"/>
    <dbReference type="GeneID" id="10601864"/>
    <dbReference type="KEGG" id="aho:Ahos_0784"/>
    <dbReference type="eggNOG" id="arCOG00608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116133_0_0_2"/>
    <dbReference type="OrthoDB" id="30763at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017158">
        <property type="entry name" value="Tscrpt-reg_CBS-contain_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF4">
        <property type="entry name" value="CRO-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037253">
        <property type="entry name" value="HTH_CBS_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="69"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="133"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="190" mass="21523" checksum="6AEB75B35C4EAF92" modified="2011-06-28" version="1">MIPDFSELKKLRQIAGLTQAELAKRVGVSQSFIAKIENGKIDPKFSIIKKIYDELLLMINIQDTAEKIMHSPVIVAHEKDDILSVVNKMENYNISQIPVVNNDEKLIGIIYDYVLLRKIISNNIKKLTASKVMSPLPPLIQKTEPINGILRLFSKYSVVLVIDDKLHPLGIITRSDLIGFLVKHKLEEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B820</accession>
    <name>F4B820_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Exonuclease-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0811</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775613.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B820"/>
    <dbReference type="STRING" id="933801.Ahos_0811"/>
    <dbReference type="GeneID" id="10600272"/>
    <dbReference type="KEGG" id="aho:Ahos_0811"/>
    <dbReference type="eggNOG" id="arCOG01019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140136_0_0_2"/>
    <dbReference type="OrthoDB" id="18579at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018665">
        <property type="entry name" value="DUF2122_RecB-nuclease-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09895">
        <property type="entry name" value="DUF2122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="148" mass="16122" checksum="D05B2F63BACA37D8" modified="2011-06-28" version="1">MKNIIVGLHNITSSQRLIDFAKIVFNLGVEKLVVTKVSGTAAQVGIPEVGKLAFKLNKSLLILPDLKDSIELLKPSSVYLVSSSARDEINIEDIRNKKDSNQILIVFSGIESGFTKIEQSLGNYVKIPKLSGDLGPEAEVAILLYTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B821</accession>
    <name>F4B821_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1973</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776760.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B821"/>
    <dbReference type="STRING" id="933801.Ahos_1973"/>
    <dbReference type="GeneID" id="10601471"/>
    <dbReference type="KEGG" id="aho:Ahos_1973"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="275" mass="31470" checksum="C7A972CDE8371734" modified="2011-06-28" version="1">MNIETPIIIRHVWKYYGKFLANEDINMTVNKGELIALLGPNGAGKTTLVKQIYGELTPSKGEILVLGSKINNKVKKRIGVIPQECTPSGSLTVRDNIYFMAKIKGLDKEKAKKNTEEFLDKLNLRQYEKKMTRDLSGGLKRRVLLAMALVNDPKVVILDEPTTGLDPEARREVWELLAELKKEGKAMLLTTHYLDEAERLADRIYFLNRRIIMEGSPAEIKSKFTTYYEVIDYSSGKTYYVKEEEISQFLNGLKGKFEVRLPNLEEIYLKVMENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B825</accession>
    <name>F4B825_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B825"/>
    <dbReference type="STRING" id="933801.Ahos_1977"/>
    <dbReference type="KEGG" id="aho:Ahos_1977"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_0_2_2"/>
    <dbReference type="OrthoDB" id="56622at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="162"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="343" mass="37743" checksum="D81717799334BA37" modified="2011-06-28" version="1">MGSNSIIISLYLLSLGLSALEIGELLSLGVLVGSFVSLILSFLGDLYGRKKFAIISRLISTVSFFFLFLGIPIAYIFSLTWGAGGLLFSLLAEKSSNLDKALGVRQSLTILFSVVGSLLPIFLEYRGILFLDVLINLVALSLLLPITEKFKGSKKLKIGSIKVVSKFSTEAIIGLGAGLVIPLMSLWFYLKFGVTGPEMSPVFALSELTLAFSSYGSVLLAERLGRVKTIVYTHSLAIVLLFLLPLSPDFLIASLIFVVRNVLMNMTSPVFESLLLRLIPEEERGRANGIVNFMESIPRAIGPSIGGYFFNEGNLFLPFFITGFLYSFATILFFIWFKREEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B837</accession>
    <name>F4B837_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776776.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B837"/>
    <dbReference type="GeneID" id="10601488"/>
    <dbReference type="KEGG" id="aho:Ahos_1989"/>
    <dbReference type="HOGENOM" id="CLU_764198_0_0_2"/>
    <dbReference type="OrthoDB" id="42889at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="42745" checksum="78DE9EA9BE1BAF34" modified="2011-06-28" version="1">MLDEDVKEILGIINSYPLVMYKIDANYINPPTYESDELYNLAKRLEDLAYPTRESDYAKVYYNFFCPVKLKNSIIRFVPIGLAYLKKYDIYEIFINTRGKIQIRRGNKEADKLVKNLLLRTISFSKFQINRGLIITADYIDKHYLEGKVKLKYVKDPIITIEEAKEILRKYKDNLSRKIEGDISVGDYLRIVKVIYETLGLDVNGELKELYRRYADGRDCGMMELPLDDAIAFKKWLSNESYCGGHPFEVVRGGLFTYGIHLYPPEDGRYVLSPGDYYEEFYKVVKEFLRRKIPFKSPELLNVLKVLTGLAEVKVNEINDFPKTIVLYEDVRDKRKIKWEEIEEVEYRREKFKKLNSNSYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B875</accession>
    <name>F4B875_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2027</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776814.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B875"/>
    <dbReference type="GeneID" id="10601526"/>
    <dbReference type="KEGG" id="aho:Ahos_2027"/>
    <dbReference type="eggNOG" id="arCOG07268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406175_0_0_2"/>
    <dbReference type="OrthoDB" id="43398at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="92" mass="10857" checksum="29FFF3A51B8CB298" modified="2011-06-28" version="1">MEWKITSKKLAVDDKKDYSCLVDDIANSDKIKELSKKYNISENTIHEFISQFLIKVFKEKKNTEEEIIEKFSIPREIVEEVKRQASSSLFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B896</accession>
    <name>F4B896_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conjugative plasmid protein (PARN3)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B896"/>
    <dbReference type="KEGG" id="aho:Ahos_2049"/>
    <dbReference type="eggNOG" id="arCOG07296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1363703_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23331" checksum="33DBF283232F0C1C" modified="2011-06-28" version="1">MTMRKFNYNEIVILNPLQTRYQMMSSRVLIPLYAAANDGIYRMNRAKRIKGFTVTLGITIGIIVLAFLVIFGIYFVFLSLSLTLPASFTDPVYWIPTLIGVGLVAGSVFGIIKLVKIGKRFGKIANELEGELVVSWSQVKTITVMNVRQENVANRPSLVLNIIAPTYKEIGDWHVLTTDGRDITIPNVDDPQNKLNYVKNRFNLNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B2</accession>
    <name>F4B8B2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved protein, Duf54</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775627.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B2"/>
    <dbReference type="STRING" id="933801.Ahos_0825"/>
    <dbReference type="GeneID" id="10600292"/>
    <dbReference type="KEGG" id="aho:Ahos_0825"/>
    <dbReference type="eggNOG" id="arCOG01042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131306_1_2_2"/>
    <dbReference type="OrthoDB" id="10874at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816:SF1">
        <property type="entry name" value="EXOSOME SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816">
        <property type="entry name" value="EXOSOME SUBUNIT, DUF54 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="150" mass="17275" checksum="148A7620AFD9204A" modified="2011-06-28" version="1">MKVTSVSFQIFSHETEDVEKIKNALNSFLQDFLKYASISENSTEGHYGDKITLIKYDFQGKISEKIVDYLFSKLDKADLLYLISTIDSRLEKSKIHIRIDKQKFIAEGKLYLRDGDDIIKIIISSVGGPKRVKEELNKIANRALHTQFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C0</accession>
    <name>F4B8C0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl37ae</name>
        <name evidence="5" type="ordered locus">Ahos_0833</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775635.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C0"/>
    <dbReference type="STRING" id="933801.Ahos_0833"/>
    <dbReference type="GeneID" id="10600301"/>
    <dbReference type="KEGG" id="aho:Ahos_0833"/>
    <dbReference type="eggNOG" id="arCOG04208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_2_1_2"/>
    <dbReference type="OrthoDB" id="372011at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="72" mass="8321" checksum="4E5380A35AC2E9EF" modified="2011-06-28" version="1">MAKYKVVGIAGRFGARYGSTLRKRWKEVMEKRYQDYQCPVCKTVGTVTRLASGIWYCKKCKAKWAGLAYTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C1</accession>
    <name>F4B8C1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">KEOPS complex Pcc1-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775636.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C1"/>
    <dbReference type="STRING" id="933801.Ahos_0834"/>
    <dbReference type="GeneID" id="10600302"/>
    <dbReference type="KEGG" id="aho:Ahos_0834"/>
    <dbReference type="eggNOG" id="arCOG04414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629887_0_0_2"/>
    <dbReference type="OrthoDB" id="37205at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9450" checksum="6ED5063657BA6738" modified="2011-06-28" version="1">MISITISINEIPEDIREIARKAILLEKIDEKYVKIDDPLTIRIKAETISRGRAIMNSYIFWLYTILRTLEEVDKSGRKNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D7</accession>
    <name>F4B8D7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps3</name>
        <name evidence="9" type="ordered locus">Ahos_0850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775652.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D7"/>
    <dbReference type="STRING" id="933801.Ahos_0850"/>
    <dbReference type="GeneID" id="10600318"/>
    <dbReference type="KEGG" id="aho:Ahos_0850"/>
    <dbReference type="eggNOG" id="arCOG04097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_1_1_2"/>
    <dbReference type="OrthoDB" id="9126at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02411">
        <property type="entry name" value="KH-II_30S_S3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_uS3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_uS3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="8">
        <location>
            <begin position="18"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="227" mass="24936" checksum="B6AE9989685D6725" modified="2011-06-28" version="1">MVNIKQYFLQKSMTKVMLDEYLAKQFYNAEYAGVDIIKTPMGTRIIIYAGRPAMIIGKGGKTIKQLAQVFEKFFNLENPQITVTNVDNPELNARVMAFRLAVALEKGYHFRRAAFITIRRIMNAGALGAEVVVSGKITSERAKYEKLKEGTVYKTGNSLETMIDRAVAIATLKPGIYGVEIIIAKPLRPVDKISFKETPAPSAGEVTVTNVKIIDENTGGAQNAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E1</accession>
    <name>F4B8E1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl14</name>
        <name evidence="8" type="ordered locus">Ahos_0854</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775656.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E1"/>
    <dbReference type="STRING" id="933801.Ahos_0854"/>
    <dbReference type="GeneID" id="10600322"/>
    <dbReference type="KEGG" id="aho:Ahos_0854"/>
    <dbReference type="eggNOG" id="arCOG04095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_2"/>
    <dbReference type="OrthoDB" id="23569at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_uL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="15209" checksum="3A97BED02A9122AA" modified="2011-06-28" version="1">MSEKLQVLGSRKALTPGLQHYSRVVVADNSGAKEAMIIGVFGYRGVLRRVPFANIADLVVVSVKKGTPEVRKQKFRAVIIRQRMPFRRPDGTWVAFEDNAVVIVNPDGTPKGTEIRGPIAREAAERWPKVASLATMVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E8</accession>
    <name>F4B8E8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl32e</name>
        <name evidence="6" type="ordered locus">Ahos_0861</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775663.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E8"/>
    <dbReference type="STRING" id="933801.Ahos_0861"/>
    <dbReference type="GeneID" id="10600329"/>
    <dbReference type="KEGG" id="aho:Ahos_0861"/>
    <dbReference type="eggNOG" id="arCOG00781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_3_0_2"/>
    <dbReference type="OrthoDB" id="372100at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_eL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_eL32_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006332">
        <property type="entry name" value="PRK08562.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="15724" checksum="EC6236A53268A2C9" modified="2011-06-28" version="1">MSINKLSKDKIYKLKQKYNSKMPDFLRYDWDKYFKLERQEKWRKTRGIDNKTRLKLKGFPPPVDPGYRKPKIIRYLHPSGLKPVIINNMKDLENIAKFKDQVIGIISSNVGFKKRLEIIKKAIEMGIKLANGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V8</accession>
    <name>F4B8V8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775744.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V8"/>
    <dbReference type="STRING" id="933801.Ahos_0942"/>
    <dbReference type="GeneID" id="10600414"/>
    <dbReference type="KEGG" id="aho:Ahos_0942"/>
    <dbReference type="eggNOG" id="arCOG05329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174016_2_0_2"/>
    <dbReference type="OrthoDB" id="39416at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="90" mass="10708" checksum="D0870CEBDE58E708" modified="2011-06-28" version="1">MPWISARGSDKIVYNQCLKLEQGKILRVESFKGDRWIEIKRKKEDEFEITEKGYNNTTYVVSSKELKTLLKRLFEIEFPRSHQVRISITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W9</accession>
    <name>F4B8W9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Na+/solute symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0953</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775755.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W9"/>
    <dbReference type="STRING" id="933801.Ahos_0953"/>
    <dbReference type="GeneID" id="10600425"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
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        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
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        <property type="entry name" value="Sodium:Solute_Symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48086:SF8">
        <property type="entry name" value="MONOCARBOXYLIC ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SODIUM/PROLINE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF"/>
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    </dbReference>
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        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
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    </dbReference>
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    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
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            <begin position="165"/>
            <end position="187"/>
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            <begin position="391"/>
            <end position="414"/>
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            <begin position="485"/>
            <end position="509"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="529" mass="56879" checksum="65E1D8862614583B" modified="2011-06-28" version="1">MNVNYLTLSVFVILFAIFSFLGFYGARWRKGDLNKLSEWGLGGRRLGTLLVWFLLGADLFTAYSFIAVPAGIFASGSLYFFAIPYVAWGFGVALLTMPRLWNVSRNKGYITAADFVKDRFNSRSLAILVALTGVVAELPYIALQIVGMEAVLAVLLAGLGISSKIVTEISLILAFIVLAAFTFVSGLRGAALTGVFKDVLIWITVITVIVLVPIEIGGFSKAFSDLSTNFVAYHLTKVTGSKVPPAMETLPIKLAPAYFSLALGSSFALYLYPHAINGSLSSESEKKLVYSTSFLPMYGIGLALISLFGILVYAVPGALSLIAKTGNGALAVPALIACTMPCWFVGLAFLAIFVGGLVPASIMAIAVANLFVRNVIKEFKPDLPPYSEAKLAKWTSTIFKFLALGFVFVVPATYAIQLQLLGGIIVSQTLPAVFLGLYTDKLESKSLIAGWAAGIISGVGLEYYVNFIELHYGVFKTSLMTTPLGPLFIAIFALAINLTITLIGTAIAYSMGWRPKSKITQEEIIKTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z7</accession>
    <name>F4B8Z7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z7"/>
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    <dbReference type="KEGG" id="aho:Ahos_2149"/>
    <dbReference type="eggNOG" id="arCOG07197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1598916_0_0_2"/>
    <dbReference type="OrthoDB" id="42930at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="166" mass="18492" checksum="EF3FEA065A139AD4" modified="2011-06-28" version="1">MREPSTLLVSIILILLPVIPGFLFNFYAFVGASIVGLAIVMYYFTSNLPWKGVKQAIVAVYFTGVFSLGLALGVFFALPIKPREFALVALVESIPFFISFAYLMKFILAKAISKDVFKVGNGYFAMLLVIIIGGLIGRFLHNFYELIILYSGFIALGIIAYLYFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B915</accession>
    <name>F4B915_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11 12">Homoserine kinase</fullName>
            <shortName evidence="11">HK</shortName>
            <shortName evidence="11">HSK</shortName>
            <ecNumber evidence="3 11 12">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrB</name>
        <name evidence="15" type="ordered locus">Ahos_2167</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3 11 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776953.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B915"/>
    <dbReference type="STRING" id="933801.Ahos_2167"/>
    <dbReference type="GeneID" id="10601670"/>
    <dbReference type="KEGG" id="aho:Ahos_2167"/>
    <dbReference type="eggNOG" id="arCOG01027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041243_1_1_2"/>
    <dbReference type="OrthoDB" id="28273at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002288">
        <property type="entry name" value="PRK01212.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="13">
        <location>
            <begin position="64"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="14">
        <location>
            <begin position="212"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE95038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="309" mass="33133" checksum="1AB8F74355BE0B80" modified="2011-06-28" version="1">MSAKAEAYSSSANLGPGFDILSMAHTAFKDIVIARTIEEKGKIIIKSNNPKIPLDPEKNSAGLATKILLQEKGIDEGIEIEINKGIPFGLGLGSSGASSVAAIAAVNELFELKLTQEEMVKYSMFGEQASSGSPHPDNVAASVYGGIIAVTSVSPVKVEKIPVNLDFKILLISPQGEREGKTKKAREMLPSKVDLSLYVKNSRYLSSLILGFIKGDKELIKQGLNDEIVEVAREPLYPHYKKIKEIALKNDAIGSCVSGAGPTVLVLYDDNTNLDKIKVESLEVCKAFGLACNFILAQIAGGVKSERLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B926</accession>
    <name>F4B926_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 9">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif2b</name>
        <name evidence="11" type="ordered locus">Ahos_2178</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776964.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B926"/>
    <dbReference type="STRING" id="933801.Ahos_2178"/>
    <dbReference type="GeneID" id="10601681"/>
    <dbReference type="KEGG" id="aho:Ahos_2178"/>
    <dbReference type="eggNOG" id="arCOG01640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_3_1_2"/>
    <dbReference type="OrthoDB" id="38099at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003067">
        <property type="entry name" value="PRK03988.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="10">
        <location>
            <begin position="22"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE95049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="136" mass="15550" checksum="3537F6F5842F72E7" modified="2011-06-28" version="1">MEDYEYLLDRVYSKLPKKSQVSETQTLPSLIIFNVGNATIIKNFSEYCDRIRREDKLCMRYLLKELAAAGSINESGQLYIQGKFSSSVINTLMERFLKTYVQCSTCKSLDTVLVKEGKIWYIQCLACGAKNPVKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B927</accession>
    <name>F4B927_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF424 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776965.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B927"/>
    <dbReference type="STRING" id="933801.Ahos_2179"/>
    <dbReference type="GeneID" id="10601682"/>
    <dbReference type="KEGG" id="aho:Ahos_2179"/>
    <dbReference type="eggNOG" id="arCOG04051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174522_0_0_2"/>
    <dbReference type="OrthoDB" id="18015at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1860.10">
        <property type="entry name" value="uncharacterized conserved protein from methanopyrus kandleri domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007355">
        <property type="entry name" value="DUF424"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04242">
        <property type="entry name" value="DUF424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11229" checksum="71C5203139125489" modified="2011-06-28" version="1">MKVILNVIRQNEHVFVNVCEKELLGKEFKDDKVILNVNKEFYGGDEVDMEYAFSLFNEATVVSIVGNYLVEEAIRRGYVHKNAILEVAGVKFAQVYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B937</accession>
    <name>F4B937_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MoaD family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B937"/>
    <dbReference type="STRING" id="933801.Ahos_2189"/>
    <dbReference type="KEGG" id="aho:Ahos_2189"/>
    <dbReference type="eggNOG" id="arCOG00534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069141_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00754">
        <property type="entry name" value="Ubl_MoaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="entry name" value="Molybdopterin biosynthesis MoaE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23404">
        <property type="entry name" value="MOLYBDOPTERIN SYNTHASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="Molybdopterin synthase subunit MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="229" mass="26121" checksum="126ED49803E525A7" modified="2011-06-28" version="1">MIKVKYFAFIKDITGKEEETLDLNCNTIDCIIKFLSEKYGRKMEEILKNGINGVKVTILVNGKISHTVADGDEVAIFPPPAGGELVKSKFDLLKEIRKFRENAPPEAGSLVVYLGFVKGIVDGHRVYELRYEAYEDYTIRRLKEIEDELMKKYHDVVKIKIIHVISNMKPGDDVLLIMCMGRGRKDSIKAVEEAVELVKNTTGIWKLEIRDDGEFWVVAGNTRVKRIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B950</accession>
    <name>F4B950_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ordered locus">Ahos_2202</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B950"/>
    <dbReference type="STRING" id="933801.Ahos_2202"/>
    <dbReference type="KEGG" id="aho:Ahos_2202"/>
    <dbReference type="eggNOG" id="arCOG04303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="OrthoDB" id="34207at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648:SF1">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-hydroxymethylpterin diphosphokinase MptE-like" evidence="6">
        <location>
            <begin position="29"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="23856" checksum="ED6AF95C9D104CF8" modified="2011-06-28" version="1">MKWISFYSKIREWLRFSYAKDYYSASLLNQLIVEDYTYLLEEIKGKEVAVVGAGPSLTSIKEINADIIISADGATNYLVKKGIIPDIVVTDLDGIEIFPEKSVYVVLAHGDNIDKIQKVKKMKYIIGTCQVMPFGRLKMFGGFTDGDRAVILAKIYGAEKILLYAMDFDSNYIGKFSKPWYDTDVPISWIKREKLKIAKNIVKEALQQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B953</accession>
    <name>F4B953_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776991.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B953"/>
    <dbReference type="STRING" id="933801.Ahos_2205"/>
    <dbReference type="GeneID" id="10601710"/>
    <dbReference type="KEGG" id="aho:Ahos_2205"/>
    <dbReference type="eggNOG" id="arCOG05951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151091_0_0_2"/>
    <dbReference type="OrthoDB" id="36729at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="143" mass="16796" checksum="DD72F69196601349" modified="2011-06-28" version="1">MLKLVENISLDILSWYGDKLKGIIYEPNYNVILVVLEDVDKISLFARGEIYNFFYNRLRELDEFKKFVVENHKSPRLYGIILSPKELSYNIPKLIYIIHKGYILYDPKGLISNIRKANVKIVEDYDKKILDLGKVKKGEVIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B956</accession>
    <name>F4B956_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775765.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B956"/>
    <dbReference type="STRING" id="933801.Ahos_0963"/>
    <dbReference type="GeneID" id="10600435"/>
    <dbReference type="KEGG" id="aho:Ahos_0963"/>
    <dbReference type="eggNOG" id="arCOG00021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054903_0_0_2"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="1">
        <location>
            <begin position="136"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="304" mass="34336" checksum="F443A1606CDF90C2" modified="2011-06-28" version="1">MLDTPLSLITDVLLPNIRALEAKRLRNLGMSQSRIASTLGVTQPAVKQYLDIQDRFYLEKLHDLGLTDDEINNILDMLTDVLLSGDIKNVMDFFTSVGLSYLSKLRFCTFHRKLNPKIPQDCSICENLYREDEEEEMNVALSMIQNEIISPLIPEVLSNIAYARKNPKSLEDIIAVAGRITKIRNLPTPASKPMWGASKHLGNILLYVSRKYPEIRSVMNIKYDEKIKKCMDELKMKYAIVGPHDYATDEIIETAVSMVFRPCLDAVVHLGGKGVEPNTYIFGKKPVDVVKKVLNIATKYSSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B963</accession>
    <name>F4B963_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative adenylate kinase</fullName>
            <shortName evidence="7">AK</shortName>
            <ecNumber evidence="7">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late maturation steps of the 30S ribosomal particles, specifically 16S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with uS11. Not a structural component of 40S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775772.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B963"/>
    <dbReference type="STRING" id="933801.Ahos_0970"/>
    <dbReference type="GeneID" id="10600442"/>
    <dbReference type="KEGG" id="aho:Ahos_0970"/>
    <dbReference type="eggNOG" id="arCOG01038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_0_0_2"/>
    <dbReference type="OrthoDB" id="8730at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="21760" checksum="A20BC083AA9E02F4" modified="2011-06-28" version="1">MTRIVISGTPGVGKTAVSKLLSSKFSLEYFHVSSFIIQNKLYESYDPLRNTYNIDDEKVAKVINSYLSDKKDVVIETIYPSLIDYADKVIILRKYPLVLYEELKRRGWNEIKVAENVEAEILGVILQEAIDWFKDKRPCQINTTNRTAEEVVNRIMNDSCEEIDWLSDEKVQDLLFTLDKVISLTENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B967</accession>
    <name>F4B967_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lysine exporter protein (LYSE/YGGA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0974</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B967"/>
    <dbReference type="STRING" id="933801.Ahos_0974"/>
    <dbReference type="KEGG" id="aho:Ahos_0974"/>
    <dbReference type="eggNOG" id="arCOG01947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825">
        <property type="entry name" value="LYSINE EXPORTER PROTEIN (LYSE/YGGA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38825:SF2">
        <property type="entry name" value="LYSINE TRANSPORTER LYSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="198" mass="22274" checksum="BD71A9DD7969BFEA" modified="2011-06-28" version="1">MKAKIYIEKVLSFHVFSYLLGVLLGLSMAAPPGPINAIMANESLRSKLHGSSVGFGAMTADFIFFLITFEVRKFIPENLLVVFYFIGGGLMIFLSYATLKAKVSNRSKKGNYFTGLIMGITNPYQISWWITVGLFMINEFGISIILPFFGGIIIWIFAFTTFINRLGNKYAKYVKIFSFIILLAFGLYMIYEGVISLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F3</accession>
    <name>F4B9F3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1404 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777014.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F3"/>
    <dbReference type="STRING" id="933801.Ahos_2228"/>
    <dbReference type="GeneID" id="10601733"/>
    <dbReference type="KEGG" id="aho:Ahos_2228"/>
    <dbReference type="eggNOG" id="arCOG07321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393509_0_0_2"/>
    <dbReference type="OrthoDB" id="36717at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22095" checksum="CBBAC6EBB87D8E38" modified="2011-06-28" version="1">MDKLDLAKYLAIIFAGISGIIYVIGDPLDKLLSYQGPVFSGALLGWYVINKYTPKDKFVEFEDSLVPVTSILIRSKSWIGFTISAFLIAFWLTPFIFKIAQEFPELYFAAFISDFIGGFIAGYLIPSLKFMEKVIIYSLGFAADIFYVMLLYIYSVIYNISQNSLLDHVLGFVYIVKFSEGILFAVYIIKKVNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G3</accession>
    <name>F4B9G3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777024.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G3"/>
    <dbReference type="STRING" id="933801.Ahos_2238"/>
    <dbReference type="GeneID" id="10601743"/>
    <dbReference type="KEGG" id="aho:Ahos_2238"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_2"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01942">
        <property type="entry name" value="ribokinase_group_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="7"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="181"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="282" mass="30548" checksum="916A63463CBA55FA" modified="2011-06-28" version="1">MRPIHLAVGKFNIDIIVKLNAFPLVDSKVNTDVMEIMPGGSATNYSVAVNRLGHSAKLLAKIGKSPLVSALLTPLAEEGVGLDFLIECEKKPNMALILLRDDGSISIVRRTDPSLLPSLEDVKKYSGMFDVIHYASISHDIIYKDPSAKIVSYDPGPYASEYEGEEVDILYVNEKEYESLKTKANAKIVVIKKGKEGAELIGSEECKVEALKVNVVDTTGAGDVFDAAFNVSYINTGSIEDSLRFASIAAGLKVTKLGGVSSPKLEEVVKMLKDVNINVKCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H4</accession>
    <name>F4B9H4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH iclR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777035.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H4"/>
    <dbReference type="GeneID" id="10601755"/>
    <dbReference type="KEGG" id="aho:Ahos_2249"/>
    <dbReference type="eggNOG" id="arCOG00374">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154365_0_0_2"/>
    <dbReference type="OrthoDB" id="46229at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="HTH iclR-type" evidence="2">
        <location>
            <begin position="191"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="251" mass="28532" checksum="0B0CABE4D020E29D" modified="2011-06-28" version="1">MKTLLLFSLLLLIGILSSLSLTATNIIIFKYPGNVIILDCSSKVVFIPKCVTHIEVNVSYVNNGCCRIILSRLPANISFIYRPQGVIKFSEPFNSKIIIELPYCTKIEYINTPPLSFNISNGKIFMTFYSGNVTILYYINEQNRNNIIYNPFLYLSIISTVGSIMIIYKIMKGRSKIEVQTQEIDERDKKIIEAIKSGADNLTKIAELSSLPRTTVYRRVKKLVSLGIINEIRENGKVRYEIKGDKNEKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K8</accession>
    <name>F4B9K8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenases of MDR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2283</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K8"/>
    <dbReference type="STRING" id="933801.Ahos_2283"/>
    <dbReference type="KEGG" id="aho:Ahos_2283"/>
    <dbReference type="eggNOG" id="arCOG01455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08259">
        <property type="entry name" value="Zn_ADH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053496">
        <property type="entry name" value="Acryloyl-CoA_Reductase_Zn-ADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041172">
        <property type="entry name" value="AcrlCoa_red_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010344">
        <property type="entry name" value="PRK13771.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="12"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="337" mass="36621" checksum="479B03B9D5EFE28A" modified="2011-06-28" version="1">MFKLMKAVVVIGHKQGYKVQEVPDPKPADDEVIIKVDRAALCYRDLLQLQGYYPRMKYPVILGHEVVGTIIQKGKKVEGFEEGDKVVSLLYAPDGTCEYCKQGEEAYCHSRLGYSEELDGFFAEYAKIKVTSLVKVPKGTPDEGAVLVPCVTGMIYRGLMRAHLKPGETVLVTGASGGVGIHAVQVAKALGAKVIGVTTSEEKAPFIKKFADHVIVGKKFAEEAKKISDVHVVIDTVGTPTFDESLKSLWMGGRIVQIGNVDPSQAYQLRLGYVILKDIQIIGHASATKKDVEGALKLTAEGKIQPIIAGTVGIDDIDKGYELLKDKNKIGKVLLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K9</accession>
    <name>F4B9K9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K9"/>
    <dbReference type="STRING" id="933801.Ahos_2284"/>
    <dbReference type="KEGG" id="aho:Ahos_2284"/>
    <dbReference type="eggNOG" id="arCOG04234">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142655_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="15190" checksum="4CCD78AE758F720B" modified="2011-06-28" version="1">MEALGAIADGRIAKVDDHYIVTSSEGDRKYTVKVEGEKVFSDDNGTKFRNYIGYPIIAVLMLEGKLPFDKRISEALKGIDWKKLNETYKNYSIVERIIKEKVSKEKGINESEIDGIIESVLNELRRHSFYKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M1</accession>
    <name>F4B9M1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775853.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M1"/>
    <dbReference type="STRING" id="933801.Ahos_1053"/>
    <dbReference type="GeneID" id="10600527"/>
    <dbReference type="KEGG" id="aho:Ahos_1053"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_2"/>
    <dbReference type="OrthoDB" id="43786at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000003">
        <property type="entry name" value="Phosphomethylpyrimidine kinase ThiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="5">
        <location>
            <begin position="7"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="395" mass="43317" checksum="BB6D9B97A2BCB8DD" modified="2011-06-28" version="1">MTIAGSDSGGGAGLQADLKTFTSLGVFGTVIVTGLTAQNTFEVTRVMEVPPDFIEAQFDAVMRDLNPRYAKTGMLASTKVIEAVKKKVEEYKIDLILDPVMVAKSGASLVTEDVVSSIKSLMKDSLIITPNKFEAEKLLGKKIESEEGLKKSAKELYEKYSVNAVVKGGSQFGLDYAIIDGEELELKGEKIETNNTHGSGDVFSASITAYLAKGFKLKDAVKKAKEFTTFAIKYSLNLGKGHGPVDPFAYPEALIEKEIAREELEDLLYFIEKKEEKTFKDLIDENDKSNVGYLTKYGDFVTLAGGIIRYLDWLKIDGPLLVNWKKNDVYEALKRSGKKIGVIISPSDKILSLAEENKIKLSESGINSDAITINGKIILVADSVEELKKKIEGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q0</accession>
    <name>F4B9Q0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MazG nucleotide pyrophosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775882.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q0"/>
    <dbReference type="STRING" id="933801.Ahos_1082"/>
    <dbReference type="GeneID" id="10600556"/>
    <dbReference type="KEGG" id="aho:Ahos_1082"/>
    <dbReference type="eggNOG" id="arCOG01084">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166059_0_0_2"/>
    <dbReference type="OrthoDB" id="49823at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42702">
        <property type="entry name" value="NUCLEOTIDE PYROPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42702:SF1">
        <property type="entry name" value="REGULATORY PROTEIN FOR BETA-LACTAMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP pyrophosphohydrolase MazG-like" evidence="1">
        <location>
            <begin position="27"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="81" mass="9393" checksum="9C936A072D382F42" modified="2011-06-28" version="1">MEIKEAQEKLKEMYLQKDKDRGLFATFTWFTEEVGELAEALLSGEKNKIEEELADVIAWAISIANLENIDVEEALRKKYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q3</accession>
    <name>F4B9Q3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775885.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q3"/>
    <dbReference type="STRING" id="933801.Ahos_1085"/>
    <dbReference type="GeneID" id="10600559"/>
    <dbReference type="KEGG" id="aho:Ahos_1085"/>
    <dbReference type="eggNOG" id="arCOG05926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230497_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="111" mass="12737" checksum="E457D09F89743651" modified="2011-06-28" version="1">MLDTLWRIIDLQLPLVKSDVETFLIKDGEITEDDLKIFNEASELIKKAYYSAEKDPNSARNLVKEALTKLESIKPKKPFPPEMRIRFDELKSSLMEVLGENKVSQTTPPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R1</accession>
    <name>F4B9R1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="11">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ctaB</name>
        <name evidence="12" type="ordered locus">Ahos_1093</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>heme b + (2E,6E)-farnesyl diphosphate + H2O = Fe(II)-heme o + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R1"/>
    <dbReference type="STRING" id="933801.Ahos_1093"/>
    <dbReference type="KEGG" id="aho:Ahos_1093"/>
    <dbReference type="eggNOG" id="arCOG00479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_1_2"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="287" mass="31171" checksum="BF64D8FEA56745C6" modified="2011-06-28" version="1">MIMATSPTVKLIYYAKLSKPRIIWLLDLAALAGAALSSKFLLIPLISVLAGGTLASGGAMIINEGIEIDKDKVMKRTSKRPTVMGYVSKKEAILVGSVMLVLGTAIGLIANPLTSFFILLGGLIYILVYTIWLKPRTPLNIVIGGFAGSAAAWAGYAAMTDTFTISSLLLGLLIFMWTPGHFWSLALKYKEDYSKAGIPMLPVLLPEKDSAKYIAISNALMAPFALILYLYVGLPYLIVTSIMTALLLYNSMVLFRNPTAEEAWKSFKISAPYLAVILLTIIILKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R8</accession>
    <name>F4B9R8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775900.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R8"/>
    <dbReference type="STRING" id="933801.Ahos_1100"/>
    <dbReference type="GeneID" id="10600574"/>
    <dbReference type="KEGG" id="aho:Ahos_1100"/>
    <dbReference type="eggNOG" id="arCOG01091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425143_0_0_2"/>
    <dbReference type="OrthoDB" id="60696at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="182" mass="20174" checksum="8D2C0CFEE5B455A7" modified="2011-06-28" version="1">MFEGIIDGLKYALKYERSILRRYLILGSFDGLLLTLGIIMSAIVEHIKVKDTEIAILSGLTAVSISSMWNSLIVEAKEKREEYKELERQMMKSLKGTIYDYGTKATIVLSAFAHGISPFLGLIVLYSYITTRNVAMVLSASSFVLFLLGLSYEGEIKDKIESGILILIAGLFTALLTYLLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S9</accession>
    <name>F4B9S9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="4 15">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 15">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 15">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">purM</name>
        <name evidence="18" type="ordered locus">Ahos_1111</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="4 15"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775911.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S9"/>
    <dbReference type="STRING" id="933801.Ahos_1111"/>
    <dbReference type="GeneID" id="10600585"/>
    <dbReference type="KEGG" id="aho:Ahos_1111"/>
    <dbReference type="eggNOG" id="arCOG00639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="OrthoDB" id="6605at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000020">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000011">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="16">
        <location>
            <begin position="42"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="17">
        <location>
            <begin position="163"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AEE93995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="322" mass="35335" checksum="891607F541FC43D0" modified="2011-06-28" version="1">MVSEYKKAGVDLNKIKEIHEYIAKEISSTYRNVLLGAGHYSGVINYNGIKLALHTDGVGTKTLLALKSGIFEPVGIDCVAMNVNDLVSIGAKPLALVDYLAMEKPMDDVIKGVMNGLVKGAIESEAEIVGGETAIMKDVINGFDLSCTALGVVDEIKDGHDVKPGDVILGLRSNGIHSNGYSLVRKLIDDGKLSFEDWKEEIMKPTKIYVKPILSVLPMIKAAAHITGGSFSKLRRVTNYHIELKMPEPQDIFKEIEKAGVSHEEMYKVFNMGIGMVVFTSPEYKDDVIKNIQKFVEVYEIGKVSEGSGITIFTYKNAVLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U1</accession>
    <name>F4B9U1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Phosphoenolpyruvate carboxylase</fullName>
            <shortName evidence="4">PEPC</shortName>
            <shortName evidence="4">PEPCase</shortName>
            <ecNumber evidence="4 5">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ppcA</name>
        <name evidence="6" type="ordered locus">Ahos_2286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>oxaloacetate + phosphate = phosphoenolpyruvate + hydrogencarbonate</text>
            <dbReference type="Rhea" id="RHEA:28370"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PEPCase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="4 5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777072.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U1"/>
    <dbReference type="STRING" id="933801.Ahos_2286"/>
    <dbReference type="GeneID" id="10601793"/>
    <dbReference type="KEGG" id="aho:Ahos_2286"/>
    <dbReference type="eggNOG" id="arCOG04435">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_517433_0_0_2"/>
    <dbReference type="OrthoDB" id="85849at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01904">
        <property type="entry name" value="PEPcase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007566">
        <property type="entry name" value="PEP_COase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02751">
        <property type="entry name" value="PEPCase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14010">
        <property type="entry name" value="PEPcase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006677">
        <property type="entry name" value="UCP006677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="509" mass="58564" checksum="899109E5858C617A" modified="2011-06-28" version="1">MRKIPKTMSTQHPDNARVPEWAKSEVIEGDDEVIEAYYSYMNLGIHEVMWDAEGKDVDTHVVRKLLSSYAEYFKENIIGQDVFLTYRLPNPRIEGAERKVFAETMESIPVAYDLAEKFYGKKVIPVFEVILPFTTDYQELVSVAKYYEKAVAGEEDIELFEGIHVRDLVGEIYPKRVEVIPLIEDKDSLLRIREIVGGYYKVIKPTYMRVFIARSDPAMNYGMLTAVLLAKFALSTLYKMKNELGIEIFPIIGVGSLPFRGHLSPKNYENALKEYKGVYTFTIQSAFKYDYDEKEVKEAIQKINENKVEEPKVFDEHEEGLLKKIVENYVSSYQPIIEALAPAINFIALNLPRRRARKLHISLFGYARSTGKVTLPRAISFVGAMYTLGIPPEIIGLSSLSKLSEEEWDFLKENYIMFNHDLNESGKYVNLDTLEYLKEIWNIDDEVINKIKEDIKFAESIGIKIGGDDYESKKHVLLSSLALLACKEKKYDEMKEYIKEMALIRKSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W1</accession>
    <name>F4B9W1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="4">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dtdA</name>
        <name evidence="5" type="ordered locus">Ahos_2306</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777092.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W1"/>
    <dbReference type="STRING" id="933801.Ahos_2306"/>
    <dbReference type="GeneID" id="10601814"/>
    <dbReference type="KEGG" id="aho:Ahos_2306"/>
    <dbReference type="eggNOG" id="arCOG01616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056464_1_0_2"/>
    <dbReference type="OrthoDB" id="9863at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003070">
        <property type="entry name" value="PRK03995.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="237" mass="26459" checksum="A71B78AFFAA4F2D8" modified="2011-06-28" version="1">MNIKVIISNVDAVGKTVRKLGYKFEEINEDVIDFKYNSGDAIVVICRHESSAKIPSLTVHHPGNIGNKTMGGEANKLGIAFPRLITSIYREISKIDINIDKVFEATHHGPTYQTVPVIFAEVGSSEEYWNNEKLVKNLVDAVLRGIERLEETTCDNVILAFGGPHYAHNVSKLAMKSCISHIISKHYISELNSNIIQQALEKTINKIDTILFDSLNQKTREKILSLINSNNISVKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y5</accession>
    <name>F4B9Y5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD-superfamily hydrolase, subfamily IA, variant 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777116.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y5"/>
    <dbReference type="STRING" id="933801.Ahos_2330"/>
    <dbReference type="GeneID" id="10601838"/>
    <dbReference type="KEGG" id="aho:Ahos_2330"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_0_2"/>
    <dbReference type="OrthoDB" id="31229at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="221" mass="25412" checksum="63BD31613DA8C960" modified="2011-06-28" version="1">MKAIFVDMGETLVKFTPRYYEAVTNAIREKGFDVDEREVFRALMQQLGNHHFPHPEVGGLSNIDFRDLFYRLGLVASDKIIKDLESRSYLSNKYELFDDAIPFLEEVKKMGLKVILVSNATSSIHKIIRELNLIKYLDGIVASCDLGVMKPHPKIFYYAKRMAGGDGIHIGDVYEIDVVGARRAYLDAILLDRFNYYPEIKENKVNNLLQAVKLIKEKLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA12</accession>
    <name>F4BA12_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphate uptake regulator, PhoU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775925.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA12"/>
    <dbReference type="STRING" id="933801.Ahos_1125"/>
    <dbReference type="GeneID" id="10600599"/>
    <dbReference type="KEGG" id="aho:Ahos_1125"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="11"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="335" mass="37826" checksum="5EE254073B44B48B" modified="2011-06-28" version="1">MPSSITRKIQLTGGSTYIISLPKSWVKQLSLNPGDEIEIFQDNNLRLILSPKARVGENRVTKATISCENATPSFAVREFIAYYMAGYDTVSFVCSRLKAEDRTIIKDMIRKRLLGSEVIEEDSNAITVQFLVNEKDLPISKAITRAASIAQNMVKDSIEALKNKDEEIAKEILERDDEVDRFYFYVSRQLTLSVSSFEILEDEGYNITQLVDIHSAIKSIERIADHASRIASLIPEVKETDTSKPVELGEKALELFKDAFGSFINERRDIANKIIERDVELANLHKQISSEILNSDIKHKSALLLVSDSFRRISRYSIDLAETTINIIAKKTTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA43</accession>
    <name>F4BA43_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
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        <source>
            <dbReference type="EMBL" id="AEE94040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10062" checksum="20ADE5A9C435B5C3" modified="2011-06-28" version="1">MSIRRIVLDVLKPIKGTTIIDLAGKLTELEGIDGVNISVTDMDVETMGLMVVIEGPNINFEEVKKVLEEDGCAIHSIDEVAAGNRLVEGRKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA49</accession>
    <name>F4BA49_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Magnesium-dependent phosphatase-1</fullName>
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        <name evidence="1" type="ordered locus">Ahos_1163</name>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA49"/>
    <dbReference type="STRING" id="933801.Ahos_1163"/>
    <dbReference type="GeneID" id="10600637"/>
    <dbReference type="KEGG" id="aho:Ahos_1163"/>
    <dbReference type="eggNOG" id="arCOG04046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010033">
        <property type="entry name" value="HAD_SF_ppase_IIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010036">
        <property type="entry name" value="MDP_1_eu_arc"/>
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    <dbReference type="NCBIfam" id="TIGR01681">
        <property type="entry name" value="HAD-SF-IIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01685">
        <property type="entry name" value="MDP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901:SF14">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17901">
        <property type="entry name" value="MAGNESIUM-DEPENDENT PHOSPHATASE 1 MDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="19300" checksum="543917C6A2EE65FA" modified="2011-06-28" version="1">MKIKVVIFDADKTLWDHYNISEFEDPIKVINKNEIEDAKGRKLKVFPDVRDTLEELKKKGIILGLATWNYPEKTQKILQILDLYKYFDVIVSRDFPYKFIMINEIFNELRNKGIKIKPEETMFVDDRRSHFGNVWLYLGNIKCVEMWKDIKCHSEILHMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N6</accession>
    <name>F4B3N6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0030</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774842.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N6"/>
    <dbReference type="STRING" id="933801.Ahos_0030"/>
    <dbReference type="GeneID" id="10599470"/>
    <dbReference type="KEGG" id="aho:Ahos_0030"/>
    <dbReference type="eggNOG" id="arCOG01407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:0016757">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="GT4_trehalose_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
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        <property type="entry name" value="Trehalose_Metab_GTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049438">
        <property type="entry name" value="TreT_GT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47779">
        <property type="entry name" value="SYNTHASE (CCG-9), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12100)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47779:SF1">
        <property type="entry name" value="SYNTHASE (CCG-9), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12100)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Trehalose synthase N-terminal" evidence="8">
        <location>
            <begin position="31"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="7">
        <location>
            <begin position="206"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE92925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="408" mass="46382" checksum="5920599847480D9A" modified="2011-06-28" version="1">MIEKYEKIIGEDELYGLVKIAEKLKDFSILHVNSTRAGGGVAEILNRMVPLMRDLGLNVDWKVIRGDNEFFRVTKSFHNSLQNGYGELPPNAFETYEKWQEINASEIPLDYDIIMIHDPQPLGLIKFRKKGKWIFRCHIDISNPYAPVWEFLKKKIENYDSMIISTPAFARDDVSIPQFIIPPSIDPLSIKNQPIPEITVKRILYKYGIDTERPLVVQISRFDYAKDPIGVIKSFKLAKEHIDGLQLAYVGSPATDDPEGEEVYEKTVKEAGDDKDIHLLMLPPYSDLEINAFQTGASVVLQKSIKEGFGLTVSEAMWKKKVVIGGRTGGIPLQIIHGITGYLVDNIEGAAHYIIHTLKNPEISQKIGENAHDHVKNNFLITRHMREYMSVMLYVTGRNEEMKSPTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y0</accession>
    <name>F4B3Y0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0049</name>
    </gene>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774861.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y0"/>
    <dbReference type="STRING" id="933801.Ahos_0049"/>
    <dbReference type="GeneID" id="10599489"/>
    <dbReference type="KEGG" id="aho:Ahos_0049"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_8_2"/>
    <dbReference type="OrthoDB" id="41015at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07197">
        <property type="entry name" value="nitrilase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="239" mass="26849" checksum="94FEB97D5FA288D0" modified="2011-06-28" version="1">MKIAVVQPSSVSSALVLTEKALLSGAQLVLLPEKWVRTVEELPLKEFQRLAIKYTAYIIPGAVEDGVSIIAPIIDSNGNIKGLAKKLHLFNKEKEKLMPGTYSTIFSISGIKVGIAICYDLDFPEVIRKMVLKGAEIILVPSKISYNGIEIWREYLRVRSLENRVAIVNANVYSPPDYPGKSVIFVPYKRNDSIVDLLTLAELGEEENFAIADINPLSYFHIRLERIKEIKDFDVMELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B416</accession>
    <name>F4B416_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dihydrolipoamide dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0087</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774897.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B416"/>
    <dbReference type="STRING" id="933801.Ahos_0087"/>
    <dbReference type="GeneID" id="10599527"/>
    <dbReference type="KEGG" id="aho:Ahos_0087"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004943">
        <property type="entry name" value="PRK06292.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="4"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="10">
        <location>
            <begin position="341"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE92980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="454" mass="50176" checksum="4BE4E54867520092" modified="2011-06-28" version="1">MIVDVLIAGAGGAGYPAAFRLDKAGLKVVMADPKGELGGNCLYQGCVPSKTLRELAHLYVRAKKLLGFNETIPFEKAQDHKDFVQETRFKQHKQELAESSVEFYKGEVEIIDNHHAVIKDEKGDVQVEYKYLILATGSEPFKPKFPGSEYCITSDDLYKYRTSIRKLPKEMVIIGGGYIALETASIMNALGTKVHVLVRSDRVLRGLDSRLVSSLLSMLDKNIDIRFNSPVIEVQKIGDKDEYKVIYSEKSEKKEISADLVMLATGRRPVYPKGTEKLGLAIGKTGIMVDETIATSVKNVYAPGDVNGRSMFFHSAVRQSLVSAHNILAGTPIDYMDFQSVPISVFTIPSIAYVGILPEEAKRRGIDIIEASYPLSKDSRAQMYDEAEGEVRLFFERGSLRIIGGWIIGIDAPTMINEIGTAVANGLTARQMANYADQHPMSNESISYAARSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B417</accession>
    <name>F4B417_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774898.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B417"/>
    <dbReference type="STRING" id="933801.Ahos_0088"/>
    <dbReference type="GeneID" id="10599528"/>
    <dbReference type="KEGG" id="aho:Ahos_0088"/>
    <dbReference type="eggNOG" id="arCOG05321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155017_0_0_2"/>
    <dbReference type="OrthoDB" id="24524at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="14192" checksum="1B982C21F0ABE04D" modified="2011-06-28" version="1">MSQLSILQIAKMQEKEREEIMSKLFQQLLQMKDEDKINTLKGLIREMAEKATDEEYLNLCKTNLKLASTLPDDVLKAFIQLRMQASSQLPKDLHDRDMKLLTKALGEVDTQIREKISRNMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B425</accession>
    <name>F4B425_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774906.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B425"/>
    <dbReference type="STRING" id="933801.Ahos_0096"/>
    <dbReference type="GeneID" id="10599536"/>
    <dbReference type="KEGG" id="aho:Ahos_0096"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080089_0_0_2"/>
    <dbReference type="OrthoDB" id="38768at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050678">
        <property type="entry name" value="DNA_Partitioning_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13696:SF52">
        <property type="entry name" value="PARA FAMILY PROTEIN CT_582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="257" mass="29803" checksum="ECB4556A0A4F1CA7" modified="2011-06-28" version="1">MIITFAPLPSYGKSHVISFLAMGLSKRGKKVLIIDLDPKLFITSFFVRQRNIYPGINSFQDIDIVSLPFFEYSFSTLNYNILSKMMQDTKINTSNYDFVFIDFKPGVSIVSMNAASFSNFILSTISTAKIKEFREGVQSIFQWMSYFNLDLKYLGNIIIAETKPEELAQQAYLGIIDALKPLPDEKINEVKKRIYPFNGTLDFVNFNTIIPVRRDLNDLKFTDKRKYPPVYRLLNYERTRSIINNLVDEFLNRINMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B457</accession>
    <name>F4B457_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF711 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776081.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B457"/>
    <dbReference type="STRING" id="933801.Ahos_1282"/>
    <dbReference type="GeneID" id="10600763"/>
    <dbReference type="KEGG" id="aho:Ahos_1282"/>
    <dbReference type="eggNOG" id="arCOG04321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064235_0_0_2"/>
    <dbReference type="OrthoDB" id="36493at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014537">
        <property type="entry name" value="UCP027893"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560:SF2">
        <property type="entry name" value="DUF711 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="UPF0210 PROTEIN SPR0218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027893">
        <property type="entry name" value="UCP027893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="37154" checksum="9CBA710ADDAD5C11" modified="2011-06-28" version="1">MKIRAVTAFVADINKDKILSIANKLNSIKEEEVLTKRISLPETNPNIEFSKLLDLVVDNSIIFSLISIKDKDKRLSQVKDVLSAGENIYANVLLTSPSYVDEIVNLITGLEPSEASRFAILINDYFLLTPYFPTSTADVARDSFALSLLYVNDFKERKAVQSLEKADALGKTIESKTGLKFIGIDISLSPWGEESVGGLIEERSGKKIFNRGHVWTVGELNRELFSSAWEAKVSPIGYSEVMLPVGEDAILSKRVEEESLTLSQLLSMTFSCAAGLDMVGIEEDKELYKNIIKDAMAIQFVKKRPYGIRIIPSRGEKKIYIKEFGYIPTIKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B471</accession>
    <name>F4B471_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpl1</name>
        <name evidence="13" type="ordered locus">Ahos_1296</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Probably involved in E site tRNA release. Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776095.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B471"/>
    <dbReference type="STRING" id="933801.Ahos_1296"/>
    <dbReference type="GeneID" id="10600777"/>
    <dbReference type="KEGG" id="aho:Ahos_1296"/>
    <dbReference type="eggNOG" id="arCOG04289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="OrthoDB" id="10382at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_uL1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_uL1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003244">
        <property type="entry name" value="PRK04203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 11 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4 11" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="24444" checksum="C60E234D0E1C2353" modified="2011-06-28" version="1">MIVPKDNLIEGLKEALSEKNNPKRKFTQSVDLIVTFKDVDMKKGDVKLREMVVLPKIPSKVKKVLVVPSFEQLESAKKAEPNVILTKEELQKLMGQKRIIKKYARQNDWFLISSESMALAGRILGPALGPRGKFPTPLPSAADVTEYITRFKHTTIVKTKDQPHTQVFIGTEDQKPEDLAENANAVLTAIEGKIRSPSIIRAIYVKTTMGKPVEIKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C5</accession>
    <name>F4B4C5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-methylhydantoinase A/acetone carboxylase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0121</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774931.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C5"/>
    <dbReference type="STRING" id="933801.Ahos_0121"/>
    <dbReference type="GeneID" id="10599561"/>
    <dbReference type="KEGG" id="aho:Ahos_0121"/>
    <dbReference type="eggNOG" id="arCOG01511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="192"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="3">
        <location>
            <begin position="526"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="642" mass="71087" checksum="EBAE5371D44D8C02" modified="2011-06-28" version="1">MIVAVDVGGTFTDIVGIDENGKITIYKGLTTPKSPEIGVYEGLKKGNFHDVKEIIHATTIATNAILGQVNLELPEVALLTTKGFKDVIEIGRQNRPELYNPFFDKPAPLVPRDKRYEINERVNAEGKIIQKVNEEEVENLIKSIKADAIAISFLHSYINPDNEKIVAQIARKYFKYVSVSHEIAPEPREYERTSTTVLNAKLMPIVSRYLEALESLLRDFSSPPIFIMASSGGLIDKEEASRRPVQIIESGPAAGVVGVAYFSEVLNIKNAISFDMGGTTAKAGTVVDHKIEITTEYEVGGKAHHGRVIKGSGYPVRFPFVDLSEVSAGGGTIIWKDSAGGLRVGPLSAGADPGPISYARGGKDPTLTDANVALGRVESLLSGEMKLDKEGAIKGLNKLGDYVEIANEAIKLANMEMGRAIRLVTVERGLDPEDFTLFAYGGAGPQYAIDLAEEMGISTVVIPPYPGLFSALGLLFADKKYEARKSYPKDLEKDFEELESKVKGKYYLRYADVRYEGQGWEIMIPVSDVKKIKEDFETIHRKIYGFTLDRDIEIITIRVFGIVPSYKVTLPKPKTDDKPSVKFRKVYFDEWIETPIYKREELPSNYEIEGPAIIEEYSSTIVIKPNWKGIVDENGFIKISKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H3</accession>
    <name>F4B4H3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type dipeptide transport system periplasmic component-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H3"/>
    <dbReference type="STRING" id="933801.Ahos_0170"/>
    <dbReference type="KEGG" id="aho:Ahos_0170"/>
    <dbReference type="eggNOG" id="arCOG01673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370735_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="725"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="750" mass="83906" checksum="BD18FC0DE3481BB3" modified="2011-06-28" version="1">MEAALPLLSLATPVSVIPNFNTPILKPCQARYTQPWVGKITFVYTYTSPTAEFEALIQGKVDTAGISEKSEYTCASCKYKNCVYVGIAPVRSFGQIVFSFNSSYITSYRCFRYAVSSLICPTNITDYVWCDGLLGQDIPYFVSPKVYAPWFNPEVVTWYEEHESYNITRAVICLSKVPGVTHVNCKWYYHGKPLVLTFLYCEDSPCQQRLAQYLKSQFALINITLCTEATTFATQIGAATTPPFVFNMTTFGWINLCPAVSSWFVIYTTPCDTGGFYNATITNLIHEADSAPTVAEDINLTKQAELDLQYCLPYIIINWANAVQGVWLPGWANYLYLCKGTQNIAIQYCYVHPVNETLTGDFIVSNLCSSLMRHLNLYSSASFYAYQIFGQLYESPAEYPFDNLTDINPADLVPLLASKWCIQKPVTETLPNGKELVNGTVLTVYFVKNATFINGYPVTACDYNFTIWYLDIPGMLGTNTFCGLTINYTYLKCHGEINGDYFGTITCLVYSQVVCPYEIKIYLNDTESVVPVDDILTDPVLYEYQCYNHIKPCNIYNCKFTPLISYGPYIWDGSSASFTNKCFTIIYNPHYFRICPLIFLNTTKEGTPYTYTATFYCYTWCSSNDSLVPHPVVGSATAWLVALDVPGVKYGPYTTPIPMTMKAPGVYTVTFNTTGLPAGVYEIVAKVTWGNGKEEFDYGSLNITPVPVTTTVPPTTTTHVSTTPVALYIGIGIVIIVIIAAIAFVIIRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K9</accession>
    <name>F4B4K9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776154.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K9"/>
    <dbReference type="STRING" id="933801.Ahos_1356"/>
    <dbReference type="GeneID" id="10600843"/>
    <dbReference type="KEGG" id="aho:Ahos_1356"/>
    <dbReference type="eggNOG" id="arCOG04213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050011_0_0_2"/>
    <dbReference type="OrthoDB" id="80661at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052199">
        <property type="entry name" value="MIPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125:SF1">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="2">
        <location>
            <begin position="192"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="361" mass="39528" checksum="56D467D7C853BCF8" modified="2011-06-28" version="1">MIRIAIAGLGNCASMLIQGIEYYKNKGDNYYEGLITPIIGNYKVTDIEVVAAFDISKNKIGKDIAEAIFQPPNITPKIVEMRKTGVKVSPGPVLDGVAPHMKSVFNPIQGQVNIEDVVNVLKESKTDILINLLPVGSEEASRAYAKAALEAGVAFINAIPVFIASDPTGKFPEEFAKRGLPIAGDDIKGQVGATIFHRAITSLFMLRGVKVEETYQLNVGGNTDFLNMKTEERLQSKRISKTKAVTSTLDNEEEIEKEGKIRIGPSDYIPFLGNTKVAYIYVKGSGFAGMPIKVEASLEVDDKSNCAAVLVDVIRAVKLALDRKIGGPLIKVSAFYFKHPPIQAKNDEEAYKWFDEFVRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L2</accession>
    <name>F4B4L2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Secretion-related protein, Snf7 superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776157.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L2"/>
    <dbReference type="STRING" id="933801.Ahos_1359"/>
    <dbReference type="GeneID" id="10600846"/>
    <dbReference type="KEGG" id="aho:Ahos_1359"/>
    <dbReference type="eggNOG" id="arCOG00452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095961_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053492">
        <property type="entry name" value="Cell_Div_Machinery_Comp"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041005">
        <property type="entry name" value="cell_div_CdvB1_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="24757" checksum="A9165F42C5333B58" modified="2011-06-28" version="1">MAKMEEFVKGWNGKQEPSLADKIKGAFKSKEPLRYKLIMAQYRLRTTLSRLDVYISKMQERDRTLFERVIEAQMNKDQARAAMYANEIAEIRKVSKQLITTQIALEQVELRLETVTELGDVFTNLVPVVGVIKELRESMKGMMPELGLELEEIEEGLQEVVIEAGDFTGGNIDYVASNPEAKKILQEASVIAEQRMKENFPELPSMVTTTQKAAPTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q4</accession>
    <name>F4B4Q4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytidyltransferase-related domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1401</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q4"/>
    <dbReference type="STRING" id="933801.Ahos_1401"/>
    <dbReference type="KEGG" id="aho:Ahos_1401"/>
    <dbReference type="eggNOG" id="arCOG01222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1212640_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.90">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050385">
        <property type="entry name" value="Archaeal_FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF357" evidence="4">
        <location>
            <begin position="12"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="3">
        <location>
            <begin position="70"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="208" mass="23989" checksum="C9C548BB54F74BCC" modified="2011-06-28" version="1">MNDRLSKINIPEYQKIIDLARQYTEDAKYYLDKGDVDTAIVDIAYAEGLLDAVLIINNKDPDSDISKKVFVGGTFDIIHPGHIEFLREASRLGRVYVSVARDKNSEKIKGRKPINDEEQRLEVVKSIRYVYDAFLGDEKDFIKSVERVKPDIIFLGPDQHVNIDEFKKELEKRGINAEIVKMPERINKWKHSSTTSIINEIVSRYCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V6</accession>
    <name>F4B4V6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775038.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V6"/>
    <dbReference type="STRING" id="933801.Ahos_0229"/>
    <dbReference type="GeneID" id="10599669"/>
    <dbReference type="KEGG" id="aho:Ahos_0229"/>
    <dbReference type="eggNOG" id="arCOG02114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1954526_0_0_2"/>
    <dbReference type="OrthoDB" id="375143at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14638" checksum="10905AB2FEEA8140" modified="2011-06-28" version="1">MSDAYLDQIITKLKEDKVIDSTVEYLYILKMIRDIWLNDEVIKDLGSLLNSILALVPIIEKLSEFLSDPEIVKIINTITSEDTIKIIQNPEKMGMTKLLSEMRNENFQRGLGILIKIIEKIGEATKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V7</accession>
    <name>F4B4V7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775039.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V7"/>
    <dbReference type="STRING" id="933801.Ahos_0230"/>
    <dbReference type="GeneID" id="10599670"/>
    <dbReference type="KEGG" id="aho:Ahos_0230"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="382" mass="42776" checksum="D572F8F1E5C1A316" modified="2011-06-28" version="1">MKKIVILGGGIGGTIVANKLARKLSSEINKGNVEITVLDKKDYHVYQPSQLLVSLGIEDYDQLIRPERSLLEPTIKFYSGNKGDIRKIDLANHKVISADGTEYNYDYLIISTGSHLAWNEIKGYREHALSVWDLDDVLKIKQTLDSFKGGTIVVNVARLPIKCPVAPVELTLMLHDLLRKKGIRDKSRIIYTYPAPGVFGIKQANDLFTEMFKERRIEIVSEYVVDYVENNTLVSQSGDKIKFDLLFGVPPHKGADVLSGLEIADRRNWIDVDKYTLNVKGYDNVYAIGDTTNLPISKAGSVADFESGPVAENIAHDILGTSGKVLYDGSVFCFCVGGIGTSSYIRYNYDFVLPVITPTTTIWWMKLMYNRMYWSLTAKAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Y3</accession>
    <name>F4B4Y3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775065.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Y3"/>
    <dbReference type="STRING" id="933801.Ahos_0257"/>
    <dbReference type="GeneID" id="10599697"/>
    <dbReference type="KEGG" id="aho:Ahos_0257"/>
    <dbReference type="eggNOG" id="arCOG01585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_4_1_2"/>
    <dbReference type="OrthoDB" id="33200at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013603">
        <property type="entry name" value="TRASH_TR_C_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08394">
        <property type="entry name" value="Arc_trans_TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRASH" evidence="2">
        <location>
            <begin position="132"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="169" mass="19177" checksum="92184DC29FBF3D71" modified="2011-06-28" version="1">MDNEINEVKLGDLEYRVLQLLKENSRMPVSEIAKELKVSRPTVSRIITSLQEKGIKFTVEYNEGVTAFVVTSKCPQDAECFELIDGNIMAVIHASDLNELEEELKKIKDEKKYLFLSHKKVGKVSVRVEMRCDYCGGAIRGSPLILKKGRKTYYACCKVCLDGLKNKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z2</accession>
    <name>F4B4Z2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775073.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Z2"/>
    <dbReference type="STRING" id="933801.Ahos_0266"/>
    <dbReference type="GeneID" id="10599706"/>
    <dbReference type="KEGG" id="aho:Ahos_0266"/>
    <dbReference type="eggNOG" id="arCOG03286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_3_1_2"/>
    <dbReference type="OrthoDB" id="36362at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053494">
        <property type="entry name" value="GH15_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041084">
        <property type="entry name" value="trehalase_H1_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF0">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="TREH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trehalase-like N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="2">
        <location>
            <begin position="202"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="568" mass="65047" checksum="55AF2A0BA327FC3E" modified="2011-06-28" version="1">MQLGFISNQRTSAIILGTSIVWFPVPKFDSPSIFTKLLDEDGGEFSILPEKIESIKQYYEHPLVLTTEVKTERGGSLKINDLLPLGETVIIRKVESDVPFTVVFKPAFKYALYRPIILRDIFVNSKGRDCVGFLYKWDGKVRRQGSFTWRFSKGKGFLVVNYATDKEHGAFSERGKALQMDFEKPFEKTIKYWESFDVKSKVYEDLYRSSVYVLLGSIYSPSGASIASPTTSLPEVIGGSRNWDYRFAWVRDSSITAEALINAGFIIEARRILNFLLSLVNFTSKPFYYPLYTVEGTIPPPEVKLMWLSGYKNSKPVRVGNAASKQIQLDVEGFFMNALYKYFEKTGDTVLIKDVFDKVEHIADWTSENWLMKDSGIWEDRGEPRHYTHSKIMMWVALDRAGKLAEYIGKEDRWKESKEKIRKWVLENCVSNGYLTRFAGSENIDSSILSAPLYGFISEKDELFLNTLSKIEKELKVGNFVKRYKSDFMGEAKHPFLLTTLWLARVYVRLGRIEEAKEIISGIEQIAGSLHLVGEHVDVEKKEFTGNFPQVFVHAEIVNLIKEIEGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z7</accession>
    <name>F4B4Z7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Z7"/>
    <dbReference type="STRING" id="933801.Ahos_0271"/>
    <dbReference type="KEGG" id="aho:Ahos_0271"/>
    <dbReference type="eggNOG" id="arCOG02702">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF3">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14390)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="2">
        <location>
            <begin position="18"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="160" mass="18492" checksum="D7228FAD84FEFAC4" modified="2011-06-28" version="1">MEDPEKFLPSIIHGVVAELGCGPGYYCQFLVKYAVKVYCVDRNKELLEIAKELAKNAIFLNEDACKTSIPSSSVDQVLFANSFHDMRDKKKVYEEVKRILKPEGEVIIVDWKKDANFGPPKSVRMSKEDYINFFSQDFELEKEFEVGPYHFGLVLKRKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B502</accession>
    <name>F4B502_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775083.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B502"/>
    <dbReference type="STRING" id="933801.Ahos_0276"/>
    <dbReference type="GeneID" id="10599716"/>
    <dbReference type="KEGG" id="aho:Ahos_0276"/>
    <dbReference type="eggNOG" id="arCOG03465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="462" mass="51247" checksum="05EC2B5E5ECC32BD" modified="2011-06-28" version="1">MIVLVYIHMFRNFYKGAYKKPRELQWVTGVLLLALTLGASFFGYSLVGDVLGIDAVDIGSSLLIGTGFPGATTIVGWLFGPGIDAVQSSNPIVRSEFFDRILGWHIIMVALISLLFGFHLMLAERYGMTPSAKEKPRVPAFYTKDEQEKFNPWWPRNFVYMLSLVLMTWGVILIVPNVLANINGATILGHKITLPLVINPYPAPQAFTTAAEHTAPYPPWIFLFLYKFVDFLLPNGLPLLPSMVIAVLIIGLVVLMLIPFFENSELMFISSRKFWTWVMSVLAYSLVALSIWGYLEPGVPAPFIQQAEIIGIPALILAIIIYLIPSKREKANATRPFGPLSILGTSVSVLLFAGTLGDFLIYPSLLGLLMLLPLGVLVYHYVTRMVRIINSANLGGGYSLSKKAAFIGIPVIFVITIFLFALLTRIPSVGIQSTYAGIDLGVILFLWGYAINLYHYLVYVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B6</accession>
    <name>F4B5B6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">D-galactarate dehydratase/altronate hydrolase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0319</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UxaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775126.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B6"/>
    <dbReference type="STRING" id="933801.Ahos_0319"/>
    <dbReference type="GeneID" id="10599760"/>
    <dbReference type="KEGG" id="aho:Ahos_0319"/>
    <dbReference type="eggNOG" id="arCOG05317">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029189_1_0_2"/>
    <dbReference type="OrthoDB" id="358791at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019698">
        <property type="term" value="P:D-galacturonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048332">
        <property type="entry name" value="GD_AH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007392">
        <property type="entry name" value="GD_AH_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052172">
        <property type="entry name" value="UxaA_altronate/galactarate_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536:SF5">
        <property type="entry name" value="ALTRONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536">
        <property type="entry name" value="ALTRONATE/GALACTARATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20629">
        <property type="entry name" value="GD_AH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04295">
        <property type="entry name" value="GD_AH_second"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-galactarate/Altronate dehydratase second" evidence="3">
        <location>
            <begin position="7"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="D-galactarate/Altronate dehydratase C-terminal" evidence="4">
        <location>
            <begin position="141"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="388" mass="41975" checksum="4AA846F3415EEA77" modified="2011-06-28" version="1">MAVTIKGYIRENGAVGIRNHVLILPLDDLSNSAAVGVSKLIRGTTVIPHPYGRLQFGRDLDLFFHILAGTGANPNVAGVIIIGIEENWANKVADEIAKTGKPVEVFPIEGYGDLRTIERASRKALEMVQDASEKQRTEVDISSIVMSIKCGESDTTSGLASNPAVGVVVDRMVDQGATVMFGETSELTGAEDIVADKMATPELREKFMKIYKEYVDIIQREGVDLLGSQPTQGNIKGGLSTIEEKALGNIQKLGHRKVNCVLDYLDPLNKSKDGGTLCFVNTSSAGAEAVTLFAAKGSVIHLFTTGQGNVVGHPIIPVIKITANPKTASQMSEHIDVDVSDLLDLKISLEEAGERIYQYMLRVMNGRLTRAEVLQHDEFSPIKLYISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C4</accession>
    <name>F4B5C4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl-hydrolase family 116 catalytic region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775134.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C4"/>
    <dbReference type="STRING" id="933801.Ahos_0327"/>
    <dbReference type="GeneID" id="10599768"/>
    <dbReference type="KEGG" id="aho:Ahos_0327"/>
    <dbReference type="eggNOG" id="arCOG03865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027051_0_0_2"/>
    <dbReference type="OrthoDB" id="43766at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="333"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="662" mass="76235" checksum="0D8DBC9806C430E6" modified="2011-06-28" version="1">MVLYSWKDEIKAGVPLGGIGTGKLEINNRGKLVNITVFGNLSSPIKSVRGFHIFISPDEGDPFFLEKDLRIRNMREVDELYYEGVYPFCYLTGKKGEIKVTLEAFSPIIPRNIKDSNIPAVGLSIKVSGSKKGTIYLSFPNFVGSTSIGRINRKVENGIIFTNIRTNEYDPRRGEVGIFSSYPTLIISQYNINVDAREAFKTFIYKDLYEDDNVWRNINKVKSDEHEVVGFWDDPAGIIASNYEENKEIRFVIAWYSRAKSYQYPYGFYYHNRFSGVLEVAQYFMKNYEELRKRTIEWKERIMNINLPDWLKDAIINSSYILTSSTWLDEKGRFGVLEAPEVFPVVGTIAGLCYEGALPVLLLFPCLEKEFIKELANVIREDGYVPHDLGIWSFDSPIEGTTAPPRWKDLNPTFILLVYRYFKFTGDVDFLRETYPKMLKAYEWMLTRSIEAEGSGDTAFDVLPIKGKNPMLLTLFIASALALRETKKVLNEKDESTDLSKLREMLNSLYNGKYFIAWEGQEGIFMAQLLGEWWTELLGLENVTDEEKISSALRYMLEVNGKASEYCTPNLVKENGEVVKISPQAYSSWPRLVFAMGWIGSKRDKRWFEIVKKEWDNIIKKGVVWNQPSRVNSINGNPEPENYLDHYIGNPSIWSFIVKEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D6</accession>
    <name>F4B5D6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D6"/>
    <dbReference type="STRING" id="933801.Ahos_0339"/>
    <dbReference type="KEGG" id="aho:Ahos_0339"/>
    <dbReference type="eggNOG" id="arCOG08525">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1648209_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="160" mass="17533" checksum="29E58CC010414F33" modified="2011-06-28" version="1">MDKLMLFTIAFLLLGIFMSFFTFSYSNSIVYCNHFKVSLSDRGGISIPYKGSTGDEVVLLGYSNSTIKVLELNPPGGAILLGSFKGNFSKTFTALTFKCITFEGCNCPASVSAKIIVYNTSFSPIGYTIAGIFIFLGLIFLGYYKALSKLRNIKVNRSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E8</accession>
    <name>F4B5E8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775158.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5E8"/>
    <dbReference type="STRING" id="933801.Ahos_0351"/>
    <dbReference type="GeneID" id="10599792"/>
    <dbReference type="KEGG" id="aho:Ahos_0351"/>
    <dbReference type="eggNOG" id="arCOG07340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079285_0_0_2"/>
    <dbReference type="OrthoDB" id="38177at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="13661" checksum="BF3CDFFB00FF2548" modified="2011-06-28" version="1">MKKFDDCCNPKRESASDFLMSQKYVGRNLEGYKCEVIKTQKGNVQIFWKKEGEKIDLRYYSPSCFTTKIALEALCEWINNGEVKNAKEAIEKLSKIKGYKITEDVKNLVYEIFTRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G2</accession>
    <name>F4B5G2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SMP-30/Gluconolaconase/LRE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776301.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G2"/>
    <dbReference type="STRING" id="933801.Ahos_1503"/>
    <dbReference type="GeneID" id="10601003"/>
    <dbReference type="KEGG" id="aho:Ahos_1503"/>
    <dbReference type="eggNOG" id="arCOG05370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_3_1_2"/>
    <dbReference type="OrthoDB" id="341532at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="13"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="283" mass="31628" checksum="62B7DFC3727DF51D" modified="2011-06-28" version="1">MEKISPYSGDLYEGPIWHPTENVLYWVNILAGTIHRLDLKKSTYLEIKLKDYVSSISPRVTGGLIATSDQGFYAVNLDGDVKEIYKVKDWDPRNRFNDGKCDAVGRYWIGTMNLEEKYPTAGFYVLDTDLSFKKVLDNVTISNGLAWSSDNKTLYYIDTPTKKIFEFDFDLEKGKISNKRVLIDLSTIQGSPDGMTIDSKDNLWVALFGGGKVIKIDTKAREIVDEIPVPTPNVTSVTFGGENLSTLYITTAKIHLTTPDENAGYVYAEKVSATGTKINFCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N1</accession>
    <name>F4B5N1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776370.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N1"/>
    <dbReference type="STRING" id="933801.Ahos_1572"/>
    <dbReference type="GeneID" id="10601073"/>
    <dbReference type="KEGG" id="aho:Ahos_1572"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_0_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="7">
        <location>
            <begin position="113"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="6">
        <location>
            <begin position="227"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="361" mass="39047" checksum="873FCD6FCF182DF0" modified="2011-06-28" version="1">MSEETELIIQSSTEVAKQLDSTSEEQGIFPRKNLELLAQQGFMGILIPKPYGMGLPARVFVDVVKNIAKVSPSTAWIYVTHVAATMAFNTFASQSLKDKYMNDLVNGKLLIGAAGTESVGGAINAVLNTTAELKGDKYVINGNKTFITGVGELDLFMIITKIVGQQKPGVILVEKGQTKVGQKFVSLGMKGVSWGELILENAEVSKDNFIVDDSVKFLGVLGRVGMLGVSAISLGLAEGAFEEALNHVKSRKLGQNTLGSFEGVQIYLAEMSAKIEAMRQLLYYAADQLSTQNPLPAVLKARIFITENALDVIDKALRITGGHGFSNALKIEKYYRDARAPMLHFQTLEISKKVLGGILVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N9</accession>
    <name>F4B5N9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated protein, Csm1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775170.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N9"/>
    <dbReference type="STRING" id="933801.Ahos_0363"/>
    <dbReference type="GeneID" id="10599804"/>
    <dbReference type="KEGG" id="aho:Ahos_0363"/>
    <dbReference type="eggNOG" id="arCOG02666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334837_0_0_2"/>
    <dbReference type="OrthoDB" id="44247at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="2">
        <location>
            <begin position="101"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="852" mass="97243" checksum="C98D1BC6299A9B55" modified="2011-06-28" version="1">MNYYIRIDPLTLYGRSKVTDSVRENVKKLLVSIIGNIDSTECRQDIKKCGKFLNEVADGIVDEINKEWEAYYFNSVFETKTVKDEELGFVNTPADTRFPGFVNSLADHMLTTSAFAVAAALSYYDNKCGELKAYDIDFSDRELLRGFVRVAALLHDIGKPPPVGHAQRTKEIVYALFKDINEKLASALSEASSRHHYGYKYTNKPQNFIEWIISYADKASASSRTFLPRNKEVFERFLDFAEKLQELYDIGGKDYINEIKNKVVESEEDSEELKTYGFLSDDENKAISLAEKLINAENDLCGGKKLLALYHFEIPSIKSYLNRGRELSIYAGYSIMIDSLIHDASDYIKSNIAKEAVISDEGGSLLAIVPSTFDSKELIKNLDLSPFDAYKENKMEFMFAEAHLGPKENWENWGSTDPYLRDSLRGFGTLINKFLSSSGTIGIPPDVSLQNEREIPLDKLCKKCRINFAEDGDECESCKKAEKFYRTFVSMVKGKGGDPLLSEKIKRLRIYKLVEELNKRNEGKISIRVWDTIDDLNKDFSGKEVDLKKRRFPTIIVGDGDNFGQLKSTATTLTQYLEINRFFTYMIYYSILYALENTALNFYKHFKVPVVEFQPILLGGDDFSLILTSQELIPFLYYMDKALVNLGGRVCRNSQLEYKDAAEMISKKKPYQWFGISVGAYIYKNTTFPIFLAREIAEDLEHISKNVSKAVFQNQFFGSGVISTIMDEKSIGLLERVKNNKGICVLGNDMREIYETIVRFENAGISSRKIFDYIKLEGDKIRIFYDVARNNSDSLETVMETINNTITKGYDIQGYLVLLGTLMSSLEQKNFEGQEYYKVPWNGQMVIQSESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P5</accession>
    <name>F4B5P5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated RAMP-motif protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775176.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P5"/>
    <dbReference type="STRING" id="933801.Ahos_0369"/>
    <dbReference type="GeneID" id="10599810"/>
    <dbReference type="KEGG" id="aho:Ahos_0369"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140524_0_0_2"/>
    <dbReference type="OrthoDB" id="44061at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09726">
        <property type="entry name" value="RAMP_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052216">
        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF3">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="63"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="243" mass="27314" checksum="996FCFD8A75D14BD" modified="2011-06-28" version="1">MAGNYTCVNASSDKTFPKNFKPRDKIQQVYIINTQFVTKTPTCTCTGEVDIDFTRRKVTYKGYTVNGKPAIMGSSIKGALNAYALMLSTPEKVGDLFGFSKLEGRIYFKDAVINTTYKEFKLGRQWRPRKPCRGIRVYPLDKPSPTSEEIYFQAIPPNTTFSTEIILINGDKEELKLVIASMGAISYGIKLGRGKDRGYGIIKLTKVEVTDMNMSKIDVGDIIKDAQNIIKQKWDNVKSAFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R2</accession>
    <name>F4B5R2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DUF1854 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0388</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775193.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R2"/>
    <dbReference type="STRING" id="933801.Ahos_0388"/>
    <dbReference type="GeneID" id="10599828"/>
    <dbReference type="KEGG" id="aho:Ahos_0388"/>
    <dbReference type="eggNOG" id="arCOG02841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_2"/>
    <dbReference type="OrthoDB" id="121502at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18563">
        <property type="entry name" value="ABC_6TM_exporter_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015005">
        <property type="entry name" value="DUF1854"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08909">
        <property type="entry name" value="DUF1854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="119"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="435"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="838" mass="96021" checksum="5DADB2B0A15E0476" modified="2011-06-28" version="1">MDFEADVNDGKFSRVKISLHDGKLEVNGNSYNLSDIEDVSLEEGLGINRIYITYKGRKVLIAEFTNRKKEGLLTLYYALKNRYDGRKEEREEEERKTRRKKDTRHFIISLISPYKYKIILGTILSSILVILSLIPPYLLKILINNVFQDREIYLFPILIASLISVNVLNVILSALQNFILNLNGQRIVNELRLKLYKHVMEMSSSFIDRYNTGRILSRLTTDISNTLWFVTWGIPSIITNVGTIVGVGVAIFLITPSLGLYALIPFPIIVLGTIMYRKRSKIAYHKLWRRTADISSLLTDTVPNIDSIKSYVKEDFESKRLSRLNGEVINAQMNVIKTNLTWFPLISASISIISVLIWYVGGEEVLAGKIELGSLVAFVTYTTMFYQPVQNLINNVIPFTQQSLTSMDRLMEVFNAESDVKIADNPKKIEVKGNVEFNNVTFGYDQVKPVIKDFSLKVKKGEKIAIVGKSGSGKSTVVKLLLRLYDPQSGEILIDGVPLKELDLKNYREQLGLIKAEPTIFYGTVEYNIRYGKIDAKPEEIVAAAMASGAHDFIMEMPFAYDTHLGERGNKISSGQKQMIEIARLFLKNPKMLVLDEATSSVDSYSERKIMDTIISQFKDSTIIMIAHRISTLYYADRIIVMEEGRIVEEGTLEELLKKRDSKFYQIFQTQIPYMEETKPRIEGKGFSYYLEMLKPVDLKILSKNKVVYEGTVYEVKSYYKPFPITRPYFLLIETNEGRYFTVDDFRKVKGSEIIEEELERKYFIPKIERIVKIDTTGDEFVWKVITNKGNTSFKTRGRNSLFKVDGKVFIIDTEDDVFEIELNAIDKKSAKMIDAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5T3</accession>
    <name>F4B5T3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5T3"/>
    <dbReference type="STRING" id="933801.Ahos_0410"/>
    <dbReference type="KEGG" id="aho:Ahos_0410"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134213_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="35"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="119" mass="14015" checksum="75DF40DA5D4292B8" modified="2011-06-28" version="1">MMGVNVIKLESLKNLIKNAYRLPREFHDKLYLMQYSRLQYWISWQARKHGMIVEFVNPSYSSVSCPKCGRKMEEVGYRWFKCNCGYENDRDVIAIMNLNGRGSLTLSTAPQMRNVRANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B602</accession>
    <name>F4B602_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR system ring nuclease SSO1393-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B602"/>
    <dbReference type="KEGG" id="aho:Ahos_1622"/>
    <dbReference type="eggNOG" id="arCOG01935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366035_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10770">
        <property type="entry name" value="Hypothetical protein VC1899 like domain (Restriction endonuclease-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013442">
        <property type="entry name" value="SSO1393-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09651">
        <property type="entry name" value="Cas_APE2256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR system ring nuclease SSO1393-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="10880" checksum="AB016F61377308EF" modified="2011-06-28" version="1">MLNFLKSKGKGASAELDSVLSAIEKLQHKKEELYVFLYTTNTWNSKLAGEVIKNYLEEEGIKSELATVKSISSEETVTLLRNYLLFLITPKYRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B622</accession>
    <name>F4B622_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776435.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B622"/>
    <dbReference type="STRING" id="933801.Ahos_1642"/>
    <dbReference type="GeneID" id="10601142"/>
    <dbReference type="KEGG" id="aho:Ahos_1642"/>
    <dbReference type="eggNOG" id="arCOG07230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590914_0_0_2"/>
    <dbReference type="OrthoDB" id="33796at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="160" mass="17489" checksum="E89C075661BB489A" modified="2011-06-28" version="1">MNLRVGELVALIIILGALSVGFLGDNQAINLTSPSISTIDHYTGIAWKLCSYYVGIAKESHFTCLYKLGNYPVAFANYTHSAGDILYVTEIAFPNSYSAHSYMLSISPSTISFTGKAYVATYISGFTEVVYLPSWGVKGAEGLARWIATLYLNTSFSKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B643</accession>
    <name>F4B643_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B643"/>
    <dbReference type="STRING" id="933801.Ahos_0443"/>
    <dbReference type="KEGG" id="aho:Ahos_0443"/>
    <dbReference type="eggNOG" id="arCOG00287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_670176_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="54"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="400" mass="45954" checksum="597D52FCD972CC2E" modified="2011-06-28" version="1">MIIQLSEAKKYLPEDYVISHEVTEEEEGENKIEIELLNDFKPVVEPLNFNQEFESIASLDSSTRYLRDISVNLVILGMSIYSNRKGFIDFPLVKEIPYIGVNTYRKILDKISAGMKFIKVKNEINYPFDENYKLDDVADEMRTEAENIGLSEVVNDHDLVILDGPIYPTPLELTEELDLSSPSRLCHQLAYASLVNKRISLLRENIIGIVKRLENSGKLWKDNQIMEEFKKRGKNIRGLKDPTILEFIDYEFCRKSGRHSYACIIGPFKIDYNLHVEGPKNCCKGDGNSTYLSNVPSKYAYYVILRKPYVPTTYFRIEGLDESFLEKGMRNALSRLSDRLIPTFIELVDNRAKRISAGLFITAYEIASSYLSIIHDDKLAYDTTVSEFLSLSQQNLTNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B685</accession>
    <name>F4B685_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775289.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B685"/>
    <dbReference type="STRING" id="933801.Ahos_0485"/>
    <dbReference type="GeneID" id="10599925"/>
    <dbReference type="KEGG" id="aho:Ahos_0485"/>
    <dbReference type="eggNOG" id="arCOG05897">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933348_0_0_2"/>
    <dbReference type="OrthoDB" id="34333at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="124" mass="14333" checksum="BB3ADC3308CD8143" modified="2011-06-28" version="1">MIKEELLSYIKGGRKEDFLKKVLSILPPSDDVSISLGKMGLYEYVIDRNGFSLIQTAEDEYLPYLSSNEKRIEFRQIPKTLIEKMDYVNVLEQLKSILEQFGGRDKKYSSLAKEVGELIEALRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B694</accession>
    <name>F4B694_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0494</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775298.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B694"/>
    <dbReference type="STRING" id="933801.Ahos_0494"/>
    <dbReference type="GeneID" id="10599934"/>
    <dbReference type="KEGG" id="aho:Ahos_0494"/>
    <dbReference type="eggNOG" id="arCOG01352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_2"/>
    <dbReference type="OrthoDB" id="6425at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF13">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="188"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="420" mass="45898" checksum="A1F65E1D7FDF08EE" modified="2011-06-28" version="1">MTVEEVLSSNLYTQQIKKLYRVGEILGLQDDQLEALATPERVIQVKIQVPSSNGKIKTFTGWRSQHNSALGPYKGGVRYHPNVTQDEVIALSMIMTWKNSLLQLPYGGGKAGIRVDPKSLSRTELEILSRNFIDALHKYIGSDLDIPAPDVNTDSQIMAWFLDEYIKVSGKVDLGAFTGKPVELGGISVREYSTGLGVAHITKLAAEKFLDGIEGKRVIIQGFGNLGSFTAKFLSEMGAKIVGVSDSKGGVIDYNGLDFNKLMEVKKTTGSVINYPEGKKVTNDELLISECDILIPGALENVINKFNAPKVKAKLIVEGANGPLTADADEIMKQRGIPVVPDILANSGGVVGSYVEWANNKMGEIIEEEEAKKLILNRMEKAFSEMYSKYNKLGDQDLRTSAMVVAVERVVNAMKARGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F8</accession>
    <name>F4B6F8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776493.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F8"/>
    <dbReference type="STRING" id="933801.Ahos_1701"/>
    <dbReference type="GeneID" id="10601201"/>
    <dbReference type="KEGG" id="aho:Ahos_1701"/>
    <dbReference type="eggNOG" id="arCOG05917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056095_0_0_2"/>
    <dbReference type="OrthoDB" id="33465at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="113" mass="12920" checksum="DD20D4175F9B65CC" modified="2011-06-28" version="1">MLEKYLEEKGYKLRVEGDRKIVDMNDYSFYIIGGNKCVFPIPLPTGKESLDDLVNMGIQYARASRLVQSLGSPVSYSVEGSSVLVIKEFKDEKELENKLIDAMDKIESLRYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G3</accession>
    <name>F4B6G3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRISPR type III-B/RAMP module-associated protein Cmr5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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    </evidence>
    <sequence length="111" mass="12155" checksum="DD6EAE7509ADDB43" modified="2011-06-28" version="1">MGNTNKLYSEILSSYVIIILIVLIMMGIMGVLAIPYYISPITYSNGGAPAGVTYLDAGIILIGLVLIGVYLIERKQFEFGVVSVLFALIVLTILIWDLYGVNDVTNIMHNI</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051929">
        <property type="entry name" value="VirAsm_ModProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34858:SF1">
        <property type="entry name" value="CYSO-CYSTEINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13108" checksum="89BE76B5025E5A0B" modified="2011-06-28" version="1">MGSIERCGIIVDGVFYEVDNISNNPYEFIMNPEEFYSIIRNGKELQAIVHTHVKNCTPSGFDVENMKIWKVYWIIISSNCIKAYKYSSSLGVIEVDINSLPFEVSDNFFVKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Q9</accession>
    <name>F4B6Q9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alcohol dehydrogenase GroES domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775385.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Q9"/>
    <dbReference type="STRING" id="933801.Ahos_0581"/>
    <dbReference type="GeneID" id="10600025"/>
    <dbReference type="KEGG" id="aho:Ahos_0581"/>
    <dbReference type="eggNOG" id="arCOG05990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978659_0_0_2"/>
    <dbReference type="OrthoDB" id="42836at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="294" mass="32480" checksum="DE098A8ABA95AF8B" modified="2011-06-28" version="1">MISIIFNQGIIPKDVVERPINNDFVLVSPDKVLLTFIENSVYLGLLWVRPWTILGSIGIGKIEAVGVDVDPTLQGKNVLILPYSKKYGGIGTEIDGLLTEKAVIPDDAIVTLPENISDKVLLYPFVSIATQIVDLVKGEKVLIIGSGLLGLLTYQYLLDSSIDVSIYTDIPGKKIQGVKEVKNLEEGKWDIVILSTMRGWARYIAERLITEDGRIIVPVFMNTWPPTLPSRSIRIYPKKMDGLLQKTEKISDKFFSENIAYSDDILSSIPTSKNGVIVNVKKALASYANSSLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U3</accession>
    <name>F4B6U3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Helicase superfamily c-terminal domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776551.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U3"/>
    <dbReference type="STRING" id="933801.Ahos_1759"/>
    <dbReference type="GeneID" id="10601259"/>
    <dbReference type="KEGG" id="aho:Ahos_1759"/>
    <dbReference type="eggNOG" id="arCOG00871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1021569_0_0_2"/>
    <dbReference type="OrthoDB" id="41184at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF6">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="40"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="272" mass="32403" checksum="51C692061809F9F7" modified="2011-06-28" version="1">MLGKEPDSKYQFTYPKEPPAIEDYYNFDLLEPKEEYLIRNINRLLEEYKSKIIIFTEYLSTLQRLESVFERHNIRFLSVNGKIRQNDRHEIIRQFKNDKEINVLLATDVAGEGLNMQFANLQINYDLPWSPLKLEQRFGRLHRYGQKKTVYLINLYVENTLDSRIVELLINKFYEISKRLGVDWIFDYIGDFVGEREILQVIQGNPISIDMNKISYIRSSVASPKEINLDYVKEFIKKNEYILNKNNDLFNIIYLIDNARQIAEECNTKYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W8</accession>
    <name>F4B6W8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776576.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6W8"/>
    <dbReference type="STRING" id="933801.Ahos_1785"/>
    <dbReference type="GeneID" id="10601285"/>
    <dbReference type="KEGG" id="aho:Ahos_1785"/>
    <dbReference type="HOGENOM" id="CLU_1574880_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="169" mass="19507" checksum="69AFD2FF58EED96E" modified="2011-06-28" version="1">MYTKWVYFPISSLAYAFIIFLAFKLFFIPLLLAYVLVYLFFAYLYIKGYRASVSLVALTYFLPLFFAELIVNIKLSFIDIIGLIIDIAGSLFAVLVLAIIRDVEDALFRIWVILASILTIALGIIYVIFYKSTSEIAWYFKILALDPIFIFNAYGAYLVLKRNNTTHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y1</accession>
    <name>F4B6Y1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776589.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y1"/>
    <dbReference type="GeneID" id="10601298"/>
    <dbReference type="KEGG" id="aho:Ahos_1798"/>
    <dbReference type="HOGENOM" id="CLU_691877_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="398" mass="45846" checksum="7512DA6BA150B841" modified="2011-06-28" version="1">MRLCIIIAIISITLILLGDVLLPFIPLISFYPTYEGIKYNSLYQNGAEIQYIGMTEVLLFSNQEYGPRISLCGPRVSLCGFLYGYKIIPVVCNFCIGHLRMGIACVNKTCCMPLYGYFVHSNFKLEYYFSIFNLTILREDNAFRVFDTIYLFNFTSKAIKTIHNKTEYILLPENITAKFTLNGDTFSVKPVKVLKYNFTCNEVPNILFKKPSHVNPQFSYYCFTPDLGLVYTTWIGGLLHPIFFHRNGTCLCAISFSLPKFIYLCRSSIPVLDGIIINLVGNLSYISKYCHPYSNAYYDLINIVYHNRRCNPLVILEHIPQHKYCQELKFAMVMFYTNVKPPLNLASWYNFGYSKYSSIIAPLFYTGLTIGIIEVIVLSGIGLYRLKKKLEREWNTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B716</accession>
    <name>F4B716_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG/DNA-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775415.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B716"/>
    <dbReference type="STRING" id="933801.Ahos_0611"/>
    <dbReference type="GeneID" id="10600058"/>
    <dbReference type="KEGG" id="aho:Ahos_0611"/>
    <dbReference type="eggNOG" id="arCOG01531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092680_0_0_2"/>
    <dbReference type="OrthoDB" id="42408at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="231" mass="26744" checksum="BEECC7596DA73B6F" modified="2011-06-28" version="1">MKTLVIKLTDEEYKQLEEEASKQGFAILSYYVKYKLLSSPSNDGINTSTTMTSSNITPQIIDEITKKLERKIQDMVNPFTAEVENLKQKIADLSEKIDELESKKSEESEAKYEKQRQYSQPSGEKKKTVMDILKSQGAIYESEVKLKNPDLFFEKIEKQGGKVLYTDKERIAVDVDFFNNFVKKLSDIHTSDDVEAQKYLTKQEHKLFQKLRQLGIIYFDNNTKAWKLSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B751</accession>
    <name>F4B751_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">N-alpha-acetyltransferase</fullName>
            <ecNumber evidence="9">2.3.1.255</ecNumber>
            <ecNumber evidence="10">2.3.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Amino-terminal acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">N-terminal acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Ahos_0647</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-terminal L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-alanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50500"/>
            <dbReference type="Rhea" id="RHEA-COMP:12701"/>
            <dbReference type="Rhea" id="RHEA-COMP:12702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-terminal L-methionyl-L-glutamyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-glutamyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50488"/>
            <dbReference type="Rhea" id="RHEA-COMP:12696"/>
            <dbReference type="Rhea" id="RHEA-COMP:12697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133359"/>
            <dbReference type="ChEBI" id="CHEBI:133360"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-terminal L-methionyl-L-leucyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12711"/>
            <dbReference type="Rhea" id="RHEA-COMP:12712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133377"/>
            <dbReference type="ChEBI" id="CHEBI:133378"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50504"/>
            <dbReference type="Rhea" id="RHEA-COMP:12703"/>
            <dbReference type="Rhea" id="RHEA-COMP:12704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.255" evidence="9"/>
    <dbReference type="EC" id="2.3.1.258" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775450.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B751"/>
    <dbReference type="STRING" id="933801.Ahos_0647"/>
    <dbReference type="GeneID" id="10600096"/>
    <dbReference type="KEGG" id="aho:Ahos_0647"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000200">
        <property type="entry name" value="N-alpha-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="13"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEE93534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="169" mass="19324" checksum="A0A250BD51B15E40" modified="2011-06-28" version="1">MEQATSNKSTNSFLIRNVRMDDIDDIIMINRLSLPENYPYYFFVDHIKDYGKAFYVAEVNGDVVGYIMPRIELGFSNLKNLPSLVKKGHVVSIAVLEAYRNRGIGTALLKASMKSMKEDYGAEEVYLEVRVSNYPAISVYEKLGFMKIKVLKHYYADGEDAYLMATYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B778</accession>
    <name>F4B778_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeal ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776613.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B778"/>
    <dbReference type="STRING" id="933801.Ahos_1823"/>
    <dbReference type="GeneID" id="10601323"/>
    <dbReference type="KEGG" id="aho:Ahos_1823"/>
    <dbReference type="eggNOG" id="arCOG08437">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154302_0_0_2"/>
    <dbReference type="OrthoDB" id="43065at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704:SF1">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="240" mass="27599" checksum="FF6EE5574016F0EB" modified="2011-06-28" version="1">MFEIERRECEEIRNLKGWRLIYGRRKVGKTYLATRCLEYDDYYLISRTLNIIHENDEIPLDDGINKIISLLKEGKKIILDEFQRLPDKYLDLLATAYPNGTLILLASSLGVLSKIVDRNSPLLGYVIPYKMGIIKFSDALASVKDPYKALLFRDPWIIEHASDWSDVERNPQSFYYITKGLIGEIFQEEDRKLTQTYEAILLEVAGGMWNSSMISAKLQAGMDINPSKASSYLHTLFKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B780</accession>
    <name>F4B780_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1825</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776615.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B780"/>
    <dbReference type="STRING" id="933801.Ahos_1825"/>
    <dbReference type="GeneID" id="10601325"/>
    <dbReference type="KEGG" id="aho:Ahos_1825"/>
    <dbReference type="eggNOG" id="arCOG03657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_4_0_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02002">
        <property type="entry name" value="TPP_BFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006203">
        <property type="entry name" value="PRK08327.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="8">
        <location>
            <begin position="377"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="522" mass="59039" checksum="AE0CEA5059A29F60" modified="2011-06-28" version="1">MDVAEFILREISKYTDKVFIVSGTDYPAFIKAELKLKNPEFVVVPHEITAASTAMGYSLGGKLGVLMVHTVPGTLNSLGIIANAYTSRIPLLVIAGKSPYTDKGSTASRTLRIHWTQDADQEELVKYVKWKFEIRDPSQVPAAISRAIQIALSEPQGPVYLAIPREVSIAKVEEKEVKMEPFYPGVPESLVERAKEMVLKASNPVIITWRAGRKESWFNSLKDFADSAKIPVLNYVGERVNYPSNGKMAVDRFNLKEADLIIEVETEVPWIPRFTDVDAKIIKVDVEPSYSYIPYYEFPCDLCIQSSVDEFFSKLRIERKPEKDLEETIERQREEKKERINKLADLKQIHPDYLSYEIGKLKLTVFNEYDLNPKYGEFNSFNSYFGDPAMGHLGWALGAGVGYKMATGKDVVITVGDGSFIFGVPTAFYYVAKKFPVLVVIFDNKSWKAVERAVKEVYPEEKFDNYPGAEIDIDDLPKTIESIGGYYEYVERPNEVEDALMRGKEKGKSGIPAIIHARIFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B788</accession>
    <name>F4B788_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AsnC family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776623.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B788"/>
    <dbReference type="GeneID" id="10601333"/>
    <dbReference type="KEGG" id="aho:Ahos_1833"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066382_0_0_2"/>
    <dbReference type="OrthoDB" id="42020at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="41470" checksum="713C81A42E2680E7" modified="2011-06-28" version="1">MLIEKEHLKLSEVFYYLQRFGDLNPRVIGAISRIKDADKLIQWLRENFQYRLAPIPRYEALGLKKYYVTIYSNYADISLYNIYELFDGMTSFILRDVINPLVLNLSIYYNSTDFDRVLDYLQDEGIIYHYDLHKVEEEKFYPIDYSNFNFERREFTGLLTSPREPFELPDLAEGFFPDEVDMKIIGKKQAKTVSSLKDISTMLGISFKDALYHTQAHVIGKGLIKGYSVYLYKPDFRLEISFSEKETLEELSRIPSMYLAYKLDDKTYYAHVFDNSSRLLDYLDFISSLKSKDTIEVYIHPFNEKYLFTASIPYEHFENGRWNFNTEIMMFRAEKLVKKIEEKDGGRQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C4</accession>
    <name>F4B7C4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1404 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776659.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C4"/>
    <dbReference type="STRING" id="933801.Ahos_1871"/>
    <dbReference type="GeneID" id="10601370"/>
    <dbReference type="KEGG" id="aho:Ahos_1871"/>
    <dbReference type="eggNOG" id="arCOG06032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109678_0_0_2"/>
    <dbReference type="OrthoDB" id="43884at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009844">
        <property type="entry name" value="DUF1404"/>
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    <dbReference type="Pfam" id="PF07185">
        <property type="entry name" value="DUF1404"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20994" checksum="9D869F90A034B7ED" modified="2011-06-28" version="1">MIRYSKEFEKKDLILPIALLVAFLNPITQGLEFTNPVIYMLDHYAIYGAGVLLGYKLLRTNFYLFLLGAIPAGFWHIPLFFALGASFVQYRTIEELTLFLGGLIAGGYIPQMKLSGKVGALAVYMLADSILSIFFILEYKQYTHECYSFLDYAPSQLPFVGISMFAVMNVILVYAIIKILRNAAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H2</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0690</name>
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    <organism evidence="2 3">
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="GeneID" id="10600142"/>
    <dbReference type="KEGG" id="aho:Ahos_0690"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138325_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="132" mass="15268" checksum="655E60FE4327FA1B" modified="2011-06-28" version="1">MRNYSGRSFNKERGQISQNEPKPLPLGEKCNYLCPYFRCNKRALMIQTKYVKGSPYKVGFCRWVGDTCITGECQYAYCEKRALLPGNRCAFAVNKRSDGDDMEKELKESDEYEDKVKDIISKKLGKKDLDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H7</accession>
    <name>F4B7H7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="8">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kae1</name>
        <name evidence="10" type="ordered locus">Ahos_0695</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775498.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H7"/>
    <dbReference type="STRING" id="933801.Ahos_0695"/>
    <dbReference type="GeneID" id="10600147"/>
    <dbReference type="KEGG" id="aho:Ahos_0695"/>
    <dbReference type="eggNOG" id="arCOG01183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="OrthoDB" id="6818at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000229">
        <property type="entry name" value="tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="9">
        <location>
            <begin position="29"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="130"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="331" mass="35833" checksum="BE71CF9532D2921C" modified="2011-06-28" version="1">MKVLGIESTAHTFGVGIAEDKPPFILANVRDTYVPKSGGMKPGDLARHHATVAPDILAKALEEAKTTIEDIDGIAVALGPGMGPALRIGAVVARALALKYNRKLIPVNHGIGHIEIGYLTTNAKDPLILYLSGGNTIITTFYEGKFRIFGETLDIALGNMMDVFVREVNLAPPYVVNGKHVIDICAENAKDLIDLPYVVKGQDMSFSGLLTAALRATKKYPIPDICYSIRENAFDMLLEATERALALTEKKEIMVVGGVAASVSLRSKLDLLAKDWNAEIKIVPSQFSGDNGAMIAYAGLLALKSGVTIPIEESVIKPRWRIDEVDIPWRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M1</accession>
    <name>F4B7M1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776683.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M1"/>
    <dbReference type="GeneID" id="10601394"/>
    <dbReference type="KEGG" id="aho:Ahos_1895"/>
    <dbReference type="eggNOG" id="arCOG07265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154341_0_0_2"/>
    <dbReference type="OrthoDB" id="34680at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021578">
        <property type="entry name" value="STK_08120-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11485">
        <property type="entry name" value="STK_08120-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="132" mass="15064" checksum="3E05DA2D3A385081" modified="2011-06-28" version="1">MKEVQLNVKHEVEVVLELFSDPDFALPRIIPGYESHTGKDTFQVIGVLGLYPYIMEGRVFKGSVIKYVFRMIDGLKGAGNIEISSIENSIKFLVDYEGDSKSMFESSFEKGIKKLSKSINEDIRLERIKRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N5</accession>
    <name>F4B7N5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776697.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7N5"/>
    <dbReference type="GeneID" id="10601408"/>
    <dbReference type="KEGG" id="aho:Ahos_1909"/>
    <dbReference type="HOGENOM" id="CLU_1850510_0_0_2"/>
    <dbReference type="OrthoDB" id="373123at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="16161" checksum="CB1EA8061C86896A" modified="2011-06-28" version="1">MEPVLVGITREGKIFEKGFATSAGFLDIQFSSEYSSFSLNDKITCVKIKNKSILNGDEIDVDCVNFLKSYVKCIEDLLNNFYHCNNKELIENVKFLNEKIKYIMYLKEDDIIIPFVGEEEMDSLSFKIMKDYKERFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R8</accession>
    <name>F4B7R8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776730.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R8"/>
    <dbReference type="STRING" id="933801.Ahos_1942"/>
    <dbReference type="GeneID" id="10601441"/>
    <dbReference type="KEGG" id="aho:Ahos_1942"/>
    <dbReference type="eggNOG" id="arCOG07331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_610612_0_0_2"/>
    <dbReference type="OrthoDB" id="40851at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="267"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="448" mass="51139" checksum="5FFD93DF09B5EF9E" modified="2011-06-28" version="1">MKSINLSLVVDENLNPLPTYKQLAISIAKVAGKSNKISFASLIGWPLLAVKSEGGGYYIFDETGKFSTSINSFILQDYNKIISMIEETSDESQVLELMNSIRWDEVRGSSIIHFDFVMDQDLKNLLKLGSSQLPLKILDRKLKDIDVQFLISDIQGAANKIISEIDLIEKTKEKISEIIAIIKGKRVEKRRQIESEYDAKIMAKNEELKKIVNDEKTKVEEDIKQYSSQLYSKLPDIEVLIAKAELDKEAGFIDSTSSVNSIKEKYLNEISEKLNEIKEKHKVEIRRLRGELTELNSMKQKDLNKINEEIKKLDELQQSITSKLNNVENSEKAILEKLQKLPRFISILAEDKAEIIVPLLIVQIDSRKVVLPPQIYKGGKKGFFGGIFKKDPSEISENVEGGEIFVNSLDITVQDNLRSYKDLIEKGLQELSEEGWNVRRSLDEYYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S3</accession>
    <name>F4B7S3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta subunit domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776735.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S3"/>
    <dbReference type="GeneID" id="10601446"/>
    <dbReference type="KEGG" id="aho:Ahos_1947"/>
    <dbReference type="eggNOG" id="arCOG01205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159724_0_0_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF1">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="114" mass="13716" checksum="C655192CA1F98DCB" modified="2011-06-28" version="1">MSWEYLRRKWEERKEILKNARNIVKLIKEICVKDIDPECRVILFGSIVRGNWRNDSDIDVLVITKKAKNEWDKAKIMSLIENSLGISDPLELHVINNKEYEGWYKRFIDVFEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S6</accession>
    <name>F4B7S6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776738.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S6"/>
    <dbReference type="STRING" id="933801.Ahos_1950"/>
    <dbReference type="GeneID" id="10601449"/>
    <dbReference type="KEGG" id="aho:Ahos_1950"/>
    <dbReference type="eggNOG" id="arCOG01608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="OrthoDB" id="372068at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000012">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF0">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="7">
        <location>
            <begin position="20"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="8">
        <location>
            <begin position="265"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="386" mass="43570" checksum="20AA55FFEF3CBF9D" modified="2011-06-28" version="1">MITVQKNVTAMVGNHAVAYAVKQAKPQVLAVFPITPQTTMLEKLAEYIDNGELKAELIKVEGEHSALAAVYGAAVAGARVFTATSSQGLLYMTEMIYWVGGERVPIVNAVATRALAEPWSIWDDHQDFFSKRDASWIMMMAENVQDAYDMTLQAFRISEDKRVILPVMMGFDGFILTHTMERLEILDDETVEKFLPLREFNLIDFNDPVNVGPIASPDDYMKIKYEAMKAMEGSKEVIEEISKEYERISGRKQYGLIECYECEDADYVYVTAGAWSGDAREAVRRLRNEGKKVGLLKLRVIRPFPKERIREALKGVQGVVVFDREYSYGYGGILANEIKSAIYNTGIEVMSVIAGIGGKDVRPLHFQKVMEDVLNGKKFEERWLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U9</accession>
    <name>F4B7U9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
            <shortName evidence="10">EF-1-alpha</shortName>
            <ecNumber evidence="10">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Elongation factor Tu</fullName>
            <shortName evidence="10">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tuf</name>
        <name evidence="12" type="ordered locus">Ahos_0740</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775542.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U9"/>
    <dbReference type="STRING" id="933801.Ahos_0740"/>
    <dbReference type="GeneID" id="10600197"/>
    <dbReference type="KEGG" id="aho:Ahos_0740"/>
    <dbReference type="eggNOG" id="arCOG01561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_2"/>
    <dbReference type="OrthoDB" id="371718at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000255">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="6"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="437" mass="48427" checksum="FB893271688B912B" modified="2011-06-28" version="1">MSAPQKPHLNLIVIGHVDHGKSTLVGRLLMDRGFLDEKTIKEAEEAAKKLGKESEKYAFLLDRLKEERERGVTISLTFMKFETKKYFFTIIDAPGHRDFIKNMITGASQADAAIIAISAKKGEFEAGMSEEGQTREHIILAKTMGINQVVVAVTKMDLTDPPYSEKRYNEVKDTVSKFMKAFGYDVSKVPFVPVVSPTGENVTHRSEHMPWYNGPTLEEALDMFEVPKKPIDKPLRIPIQDVYSKSGVGTVPVGKVESGVLKVNDVVVFMPVGKKGEVRSIETHYTRLEKAEPGDNIGFNVRGVEKKDIKRGDVAGHLDNPPTVADEFTARIIVVWHPTAIAVGYAPVLHVHTASVACKITELVQKLDPKTGKVVEDHPQFLKQGDSAIVKFKPIKPLCVEKFSDFPALGRFAIRDMGKTVGVGVITDIKPMKVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V7</accession>
    <name>F4B7V7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">Ahos_0748</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775550.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V7"/>
    <dbReference type="STRING" id="933801.Ahos_0748"/>
    <dbReference type="GeneID" id="10600205"/>
    <dbReference type="KEGG" id="aho:Ahos_0748"/>
    <dbReference type="eggNOG" id="arCOG01762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_0_2"/>
    <dbReference type="OrthoDB" id="6009at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019969">
        <property type="entry name" value="RNAP_Rpo2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006335">
        <property type="entry name" value="PRK08565.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03670">
        <property type="entry name" value="rpoB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="18">
        <location>
            <begin position="19"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="165"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="409"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="516"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="600"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="15">
        <location>
            <begin position="648"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="1020"/>
            <end position="1110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AEE93634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1123" mass="126287" checksum="62F6971689EE3EB9" modified="2011-06-28" version="1">MLSVDDRWAIVESYFKSRGLVRQHLDSFNDFIKNKLQEIIDEQGEIETEIPGLKIKLGKIRVGKPRVREADRGDREITPMEARLRNLTYAAPIYLTMIPVENNIEGEPTEVYIGDLPIMLKSVADPTADLPAEKLVEIGEDPKDPGGYFIINGTEKVIVTQEDLATNRVLVDEGKSGSNVTHTAKIISSTAGYRVPITIERLRDSTIHVSFPAVPGRIPFAILMRALGLETDRDITFAVSFDEEIQNELLPSLEQASSITTQEDALDFIGNRVAIGQKRENRIERAEQVLDKYFLPHLGLTPADRKKKAYYLGYAVSKLIELQLGRREPDDKDHYANKRLKLAGDLFSSLFRVAFKAFVKDLVYQLEKSKVRGRRLTLNALVRPDIITERIRHAMATGNWVGGRTGVSQLLDRTNWLSMLSHLRRVISSLARGQPNFEARDLHGTQWGRMCPFETPEGPNSGLVKNLALLAQISVGINDKIVEKMLYELGVIPLEELIRKIDEGEDPSSYTNWSKVILNGRLVGYYPNGEELANKMREKRRAGEISDEINVAHIVTDTYDEVYVNSDSGRVRRPLIVVKDGKPLVTEEDIQRLKKGEITFDTLVKEGKIEFLDAEEEENAYIALEPSKVTPEHTHLEIWTPAILGITASIIPYPEHNQSPRNTYQSAMAKQALGLYASNYQLRTDSRAHFLHYPQKPLVQTRALDIIGYNNRPAGENAILAVMSFTGYNMEDAIIMNKSSVERGMFRSTFFRLYSAEEIKYPGGQEDKIQLPEPGVRGYKGSDYYKSLDENGIVPPEVEVKGGDVLIGKVSPPRFLQEFKELSPEQAKRDTSVITRHGEKGIVDLVLITETSEGNKLVKVRVRDLRIPELGDKFATRHGQKGVIGMLIPQADMPYTVKGIVPDIILNPHSLPSRMTLGQIMEALAGKYAALSGKTVDATPFYNTPIEELQKKLLDFGFLPDGTEVVYDGRTGQKVKGRILYGVVYYQKLHHMVADKMHGRARGPIQILTRQPTEGRAREGGLRFGEMERDCLIGYGAAMVIKDRLLDNSDKTTVYVCEQCGYIGWYDKNKNKYVCPIHGDKANLYPVTISYAFKLLLQELMSMVISPRLILGDKVSLKGDNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W3</accession>
    <name>F4B7W3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps28e</name>
        <name evidence="6" type="ordered locus">Ahos_0754</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W3"/>
    <dbReference type="STRING" id="933801.Ahos_0754"/>
    <dbReference type="KEGG" id="aho:Ahos_0754"/>
    <dbReference type="eggNOG" id="arCOG04314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000145">
        <property type="entry name" value="30S ribosomal protein S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003080">
        <property type="entry name" value="PRK04007.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="83" mass="9448" checksum="EE325B844674FFF5" modified="2011-06-28" version="1">MDMSEKKAETSPIIEEFGFPAEVIQILDRTGVTGEVTQVRVRVLEGRDKGRILTRNIKGPVRLGDIVILRETEREARKLTTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X9</accession>
    <name>F4B7X9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine ammonia-lyase</fullName>
            <ecNumber evidence="3">4.3.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775572.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X9"/>
    <dbReference type="STRING" id="933801.Ahos_0770"/>
    <dbReference type="GeneID" id="10600229"/>
    <dbReference type="KEGG" id="aho:Ahos_0770"/>
    <dbReference type="eggNOG" id="arCOG01431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_2"/>
    <dbReference type="OrthoDB" id="9915at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000007">
        <property type="entry name" value="L-threonine dehydratase catabolic TdcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="330"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="405" mass="44038" checksum="C3DC129EE911A6C7" modified="2011-06-28" version="1">MTYIKYFDEIVKIRERISPYIHETPLDYSTTFSRMANSNVYLKLENLQKTGSFKVRGAFSKLLTLSDEEKKRGVIAVSAGNHAQGVAYASKVLGIKSVIVMPETAPISKYLSTKSYGAEVILYGSYLHESMSKAEEIIKERNLVLVHPYDDINVILGQGTLGLEILSQISPDIVVVPVGGGGLISGISIALKAKNRNIKIIGVQSSASPSLKISKDLGRLTEIEPSYSIADGILVKTPSKLTFDIISELVDDIVLVDDDEIACAMSLLLERAKTLVEGAGAASLAAIISSKIKLNSSQKVVSILSGGNVDLSLLSHIIEKNLYKIKRIVKVKVIVPDKPGYLNKVLSYVAKIRGNIIDVVHDRISSDVKPGYTKIYVMFEISSTDAISELILDLAKEGIDAKIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y3</accession>
    <name>F4B7Y3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA methylase, NOL1/NOP2/sun family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0774</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775576.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y3"/>
    <dbReference type="STRING" id="933801.Ahos_0774"/>
    <dbReference type="GeneID" id="10600233"/>
    <dbReference type="KEGG" id="aho:Ahos_0774"/>
    <dbReference type="eggNOG" id="arCOG00973">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_7_0_2"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF74">
        <property type="entry name" value="TRNA (CYTOSINE(48)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="51"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="335" mass="37997" checksum="CC52BE03920C8535" modified="2011-06-28" version="1">MSTIENYIKKYDKVFEVSPSLKAIELSRKYGFLDYMVERYLHIFRHEAEDFLYSCNFPLKKSIRCNTLKIDCNELESRMEQKGFKLEKVSWLKHGYIVKKAPPKPSLGATLEYLQGYYYIQGLASMVPAYVLNPSSSDTVLDMAAAPGGKTTQLSQIMNNEGLIVAVEKSRLRVTSLLSNISRLGVRNVLLLRSDVRTLEKTTLSFDKILLDAPCSGEGLIPEDQSRKTKTSLDELKRFSFTQLQLIYTAYKLLKKGGELVYSTCSIAPEEDEMIVNFAIEELGMKAIKISGYPAENGIVEYNGVSFNEEVSNCIRFYPHKNGTEGFFICHLKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B813</accession>
    <name>F4B813_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylate--amine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775606.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B813"/>
    <dbReference type="STRING" id="933801.Ahos_0804"/>
    <dbReference type="GeneID" id="10600265"/>
    <dbReference type="KEGG" id="aho:Ahos_0804"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_2_0_2"/>
    <dbReference type="OrthoDB" id="35908at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF3">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="27517" checksum="10C98C3DCA7AAE0D" modified="2011-06-28" version="1">MKVILKGIAEDELKGSTFITGFRTIGETGYLASRYIVLKRKLQRIGFITTKYLRDVTFLDEYGIATPFDLFYDKELHTIVLLNHLLPFQREWNSFAKDTIMWLKKLEVKNIILIGGLDKRYKESSEELRWLKTSKSTINLPYPELEKQLIMVGPLALFTIYSEIEDLPAIVLLPYADRERIDPAAAAIAVDAINKILGTNIDVTELYEDAKKLEEELQKQLENIQKEISRSGLDRHYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B832</accession>
    <name>F4B832_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B832"/>
    <dbReference type="STRING" id="933801.Ahos_1984"/>
    <dbReference type="KEGG" id="aho:Ahos_1984"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="9"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="34242" checksum="A39DF466757EADFF" modified="2011-06-28" version="1">MAYEKDFMIRVENLVKFYGHFQALKGESFTVNDGEIVGFVGLNGAGKTTTIKIATGVLYPTSGDVIIDGYSITKDKKKASENVGWVPELPNYEQDVKALDYFVYIAGYYKIPTIEARSLGKRLFEEVGLAGRENDKLKNYSQGMKKRFALAVSLISNPKNFLFDEVLNGLDPEGIQFFRNLALRLKKEGKAVLFSSHILSEVESLADRVVFLHKGKIVKEEKMEDLRKLVSANALRIILNSPSEEALKACKDFGEPILNNGVIIIQNFKGDLEDVVRALKPFGILDIGYVRQDLESLFFQILGGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B838</accession>
    <name>F4B838_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776777.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B838"/>
    <dbReference type="GeneID" id="58788769"/>
    <dbReference type="KEGG" id="aho:Ahos_1990"/>
    <dbReference type="eggNOG" id="arCOG09710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_547034_0_0_2"/>
    <dbReference type="OrthoDB" id="377394at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="498" mass="57091" checksum="D3656D17DD83AF3F" modified="2011-06-28" version="1">MDKLRLEIILGSLLFILSFFPHYCLYLLTIISLGILLRLKYFPPFVSSILLLYSPFHALITLLTFPLLYIDRKFSLSFSLATLFSLLTQNYLILFLAGALEKRGLIISGIMFLILSALTMHEEYGNIAYFLLVAGVISAIIEGKISLRTSAVIYLSALAFYFKLPFLIPAIVSFSPLVSLLFSPFYPYFAIISIKYAENFRKLKFLNFIPPALSFINPFLSFSSLYDVIRKREIFLYILLPLFSSIYFVIEGEYTQEEIISISIIFLFTLNILRDYYKMIISFMDKYSQFIISLALDFIAYKFFLFNLEIFLTIVLSSIVISLPKLKISVYPFFASIISLVNPFVGLSAVQRLSSLFVFIPLFFYIEMHYSVYSIVFYIIGASLGIVFSIRDYNMERNIVFVIFALLLTLDDVVLRNYIYIAFSLVSAVLIFLFRRNLNISEILALYFASFPLSFISGFFILLKGKLNLCIILVTEAVISVLVLKIVSITSPILSQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B847</accession>
    <name>F4B847_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776786.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B847"/>
    <dbReference type="STRING" id="933801.Ahos_1999"/>
    <dbReference type="GeneID" id="10601498"/>
    <dbReference type="KEGG" id="aho:Ahos_1999"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056492_0_0_2"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="42"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="324" mass="35921" checksum="8BA2C30E9F0D9038" modified="2011-06-28" version="1">MNLKIVGLAIVILVIIGGVAVYELIKKPSTVEQPSLTGKYIRIVSLTPSDTQDLIALGLGKDIVGVDYYSYTLLKLVNKTSCLPSNVTVFPQIYPVNISGLVELNPTVVVGEKGLLYECINSMQEAGLKTYLTNADYASNFYQIESCICSLGKYFNETNASKELVNWMNSKLQEFSSSGNKTVAYIDWICPNYYFWTAGGNVFINSLIQLGGGINAFGECINYEKLCPGKLIEANPDILVVNVIYNISYTKYLLCHIPGIQNVTAYKDGNIYFLCCQSSYLIEEPGPLAVYAVLLFHDIINGTAPHQITWSWIYNCIKPELPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B866</accession>
    <name>F4B866_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776805.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B866"/>
    <dbReference type="GeneID" id="10601517"/>
    <dbReference type="KEGG" id="aho:Ahos_2018"/>
    <dbReference type="HOGENOM" id="CLU_718865_0_0_2"/>
    <dbReference type="OrthoDB" id="372860at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="384" mass="44805" checksum="DEB2E3B727258338" modified="2011-06-28" version="1">MVTYLVEKEIGSKEEPKVRIIRRTDDRLSYTFDSSRISRLYIPYTMKERMDEIIETVNKSPFTKLSRISSEELDKVLLILRRYPNLFNSIDFFSADDIVLSEFDEEGRINGRKRKFKIKDKIYEIIGNKSIIVKEKYYEIAKKINALKVEITFNSYDRGFKISSLGNLLMVKSKKGYKEYFFDRFSVIIERMLKKEDIETVKKYKGKIFHFTKDYGTTLIDGIALKKFYIDGKESGIEELRKIAGEFSAEISKHSFSWFKGHEITTSQVQIPHTQFDYSVDDLNLLKDVSNLDKGYFVYGNLNIYSYDYGFTIKLKDGKPTFFKLNESVKSIDAKNFDIVASEVPPSGRGKVLIYTTLGFKKENNLNPIKFISKDGIRMKKISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B869</accession>
    <name>F4B869_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776808.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B869"/>
    <dbReference type="GeneID" id="10601520"/>
    <dbReference type="KEGG" id="aho:Ahos_2021"/>
    <dbReference type="HOGENOM" id="CLU_1850512_0_0_2"/>
    <dbReference type="OrthoDB" id="43559at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="16413" checksum="697EA061658B5D7E" modified="2011-06-28" version="1">MTVNSRSILKDERERVTNIAFFLKRFMTGKEKFSSLDDLVKFIDSLAKKFEFLSTVKNWEKWKLELLLKKVNFVARSIRKEKKLKVSLERKFKGIKIQKVEEYDADRFTVFYMHEGKSKAVSGSAREIKQKLLKEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B877</accession>
    <name>F4B877_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B877"/>
    <dbReference type="KEGG" id="aho:Ahos_2029"/>
    <dbReference type="eggNOG" id="arCOG03670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_307901_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="851"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="877" mass="100404" checksum="70E77AA5256596EF" modified="2011-06-28" version="1">MSREILFLALIILTVITPLGICNFHVVQRIPGEPPPVIKTYAVAYPIFEKYVSLNNCSQEIIVQDNCPGIYCLYVFTPQTYCLWSLGHSENAIFSTKITKGTYVIPLNKGNYVVVINHFLGKDSNINVNVAPRSFCSFIMSNRLASPRGIVSYGIYDYAGELQNYCIRTNSILGYFNISCFEPKGCLASLQLNAVLLINGGNDYEYFLQNIIKFENCFHWFYLEDNIWNFSSYRANIYHVEGKGIINSYCNRTYYCYSPPCLFDIKYSLPLAGYLIINVTTVQGKGVYVTFGFSKIQNGQEILPPIFTYYDKVFISCPNVISACIVIKPNFTKSLNVFSAGLVFGSYEQLSTVSFNNLNSTLALLYCNQGWKPVPSFFSFCVNSLESACNLNFKAAKSKILVSTNVQCSNSVIDMPHVNVPFTFVDYNNKPIYIEKPLNVSLPRTIYICKNEKQCLYKVYLCINGIRQQINDGYIIYPRCYFISINITAFYNTYYFIRIFYPNGTFCSWFLSCSKICLPKIIQVCKFIRYNLNQNSTIMATGPLNITPIYELQYLVTINLPTNKTEEWVNNGTIIQIPKTIYINAGTRYYLLSSNESYVSKPLNITPIYELQYLVKLILPNGTIEEWVNNGTIIQIPKTIYINSTSRYYLNNTNTSFIICRPMIIKPCYKLQYLVKLILPNGTIEEWLDSHSDLILPKIIQINDTRYILNEQNNIIEILGCRILCPKYDVYYLVKLILPNGTIEEWVKKDCVIILPQYIYISSYERYVLNSTQFVIVHSPLVIQPEYIKQYLININGISYWYNQGAKLLIKIATTPFFIVKWVGSIKVTNGEIITINGPLDLKAEILINPLFEYLGIAGIIAIIVLAITNVIRHKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A2</accession>
    <name>F4B8A2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl21e</name>
        <name evidence="7" type="ordered locus">Ahos_0815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
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    </evidence>
    <sequence length="99" mass="11431" checksum="2CE8FDD692A1D850" modified="2011-06-28" version="1">MVSHSKGNRTRSRNLLKKSPRERGAIPSLSSLMYDYKPGDKVIIKINSSIHNGMPHRRFQGRVAEVVGKRGRAYELKLRVGNKEKLIIVRPEHLRLFKQ</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="entry name" value="PHP domain-like"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="187" mass="22089" checksum="25260D3EDF2D38A2" modified="2011-06-28" version="1">MLIEPCILNFKIKHFLKKAGYDEAFIESKDSNSRISRITIVAKDKNTLFNNIKLYSGNKLIFCKPLTEEILRICVSYNKVNAITVDNVNFHLFKRRAMLNLIRLHDKTIEVILPYSSSYIIYKFIIWGYKWIRNILFSSCAKDFNEIWNPLSKVNYLILHGADYEMATYWVFKSPQVFLNNVSSNYY</sequence>
</entry>
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    <accession>F4B8J4</accession>
    <name>F4B8J4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2075</name>
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    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="KEGG" id="aho:Ahos_2075"/>
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    <dbReference type="HOGENOM" id="CLU_090242_0_0_2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
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        <property type="entry name" value="HTH-Siroheme_Decarb"/>
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    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE94946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="30066" checksum="2B6692FEBC7E5C5C" modified="2011-06-28" version="1">MDQINKKILFYLLKDYRTSQRSIAKMIGISSPAVNYRVDKLIQDKVIKKLSLYVNPNFYGKYHAYVAFKNIKDWEGEYVFKVNCLEETNVYEIEGSSLDDLKKKIDIMSESLDYPRMVYIPKQQPYNPSNFDLKLVSIMKEEPTLDTMYLADKMKVSTKTVRRHIRYLYSRNFIRLIPEIDLTKAGLIMYAVFTHNVEIGRKFFARQMFREISDDTAGIFVNVGDSLEEVKEEFFKFREFDKDADLMIAIDYSVS</sequence>
</entry>
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    <accession>F4B8N7</accession>
    <name>F4B8N7_ACIHW</name>
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        <submittedName>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N7"/>
    <dbReference type="STRING" id="933801.Ahos_2118"/>
    <dbReference type="GeneID" id="10601618"/>
    <dbReference type="KEGG" id="aho:Ahos_2118"/>
    <dbReference type="eggNOG" id="arCOG02699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_5_4_2"/>
    <dbReference type="OrthoDB" id="31083at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050709">
        <property type="entry name" value="Biotin_Carboxyl_Carrier/Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45266:SF3">
        <property type="entry name" value="OXALOACETATE DECARBOXYLASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="89"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="167" mass="18494" checksum="FFB0DCF5FF12E488" modified="2011-06-28" version="1">MKLYRVYSETGDTYLMAVESKGNVDKIKTEANEFEIEYLGKGTRDNEYLFKVNGEIHHVFVDNGYVFVDNASVFKLERATELPTKEGESVEEMIKGREGEVISPLQGRVVSIRVKEGDAVNKGQPLLSIEAMKSETIISAPVAGVVEKILVKQGQGVKKGDTLVIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S9</accession>
    <name>F4B8S9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775715.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S9"/>
    <dbReference type="GeneID" id="10600385"/>
    <dbReference type="KEGG" id="aho:Ahos_0913"/>
    <dbReference type="eggNOG" id="arCOG08332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968608_0_0_2"/>
    <dbReference type="OrthoDB" id="40770at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="60" mass="6772" checksum="598E1990A8C501F3" modified="2011-06-28" version="1">MEDYRKIFEGLAYTIIEDDEASIVLLEGKPIAASCIEHGNHDLLDLNCPHVENLLKKVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W7</accession>
    <name>F4B8W7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0951</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W7"/>
    <dbReference type="STRING" id="933801.Ahos_0951"/>
    <dbReference type="KEGG" id="aho:Ahos_0951"/>
    <dbReference type="eggNOG" id="arCOG00558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="49"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="233"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="370" mass="41963" checksum="1FC12279BC35EA83" modified="2011-06-28" version="1">MVEIYNLIDNILNSGVEACVMFKNLSEELTKALQEANYVNPTKVQELAIPQMLQGKSVLAQAKTGSGKTAAYLIPIIEKEEQALVLTPTRELAEQVANEARKLGKYKKMSIGVIIGGVPYSGQDKEAKKDIVIGTPGRILDLWGKGYLDLSRFSVAIVDEVDRMFDMGFIDDVRMILKNANPDMYGFFSATVPEEVESLAKEFSPNIEFLKVDDIKPVEIEHLFYEVKGWRDKISKLKDELDDNEKTIVFVNTKKKVEELYEELRDDFEISLLHGDLPQRVRLRNLSSFRRGKTNVLLSTDLASRGIDVIDVTKVINFDTPRDVETYIHRVGRTGRMGRKGIAITFYTRRDAQLILKIKDLIKGKKLEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W8</accession>
    <name>F4B8W8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MarR family transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0952</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775754.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W8"/>
    <dbReference type="STRING" id="933801.Ahos_0952"/>
    <dbReference type="GeneID" id="10600424"/>
    <dbReference type="KEGG" id="aho:Ahos_0952"/>
    <dbReference type="eggNOG" id="arCOG03748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922885_0_0_2"/>
    <dbReference type="OrthoDB" id="29074at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14876" checksum="5E19563CF547B1D0" modified="2011-06-28" version="1">MNLTDKILALLYDRGELTSNEIADSLREDEEDVELTLKGMEKEGLVFEKEKGLIFKKKVYSLTPTGLEKAKKAKEELEEKANKLVTAIQQGEDPQVIVQEYADLIPLMLAMSLIDMMLLQDLMFIDFMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z1</accession>
    <name>F4B8Z1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-methyl-5'-thioadenosine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5'-methylthioadenosine phosphorylase</fullName>
            <shortName evidence="5">MTA phosphorylase</shortName>
            <shortName evidence="5">MTAP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mtnP</name>
        <name evidence="7" type="ordered locus">Ahos_2143</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776929.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z1"/>
    <dbReference type="STRING" id="933801.Ahos_2143"/>
    <dbReference type="GeneID" id="10601646"/>
    <dbReference type="KEGG" id="aho:Ahos_2143"/>
    <dbReference type="eggNOG" id="arCOG01327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_2"/>
    <dbReference type="OrthoDB" id="7681at2157"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00873"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000012">
        <property type="entry name" value="Probable 6-oxopurine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006334">
        <property type="entry name" value="PRK08564.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006599">
        <property type="entry name" value="PRK09136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF3">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="6">
        <location>
            <begin position="8"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="57"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="5">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="269" mass="30462" checksum="FEA575F010F0C4D9" modified="2011-06-28" version="1">MLEYEKVPIAIIGGSGLYDPKIFSESKEIKVYTPYGDTSDLITIGTVEGKKVAFLPRHGRRHRIPPHKINYRANIWALHELGVKWVISVSAVGSLRMDYKPGDFVVPDQFIDMTKKREYTFFDGPVVAHVSMADPFCNHLRKIIISAAKDLGITTHESGTYICIEGPRFSTRAESRVWKEVFKADIIGMTLVPEVNLACEMQMCYATIATITDYDVFAEVPVTAEEVTRVMYENTEKSRKLLYEVIKRIPDKPDERECSCCNSLKTALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z4</accession>
    <name>F4B8Z4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-L-glutamate ligase, RimK family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z4"/>
    <dbReference type="STRING" id="933801.Ahos_2146"/>
    <dbReference type="KEGG" id="aho:Ahos_2146"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="25"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="23178" checksum="B6961A17A8141414" modified="2011-06-28" version="1">MEKSGVVFINNPQSVLLARDKFESLMKLKRTGVPVPPTAMVEDPFEVMRLVEKWGEVVIKPVIGSLGLGSVRASDPDIAFRIAKAILSVNQPVYVQKYVKKPDRDIRAFVVGDRLLGSIYRISQGSWKTNVAQGAVVQVFKPSSEIEELSLKATKVLGLDYAGIDIVEDLEGGLKILEVNAAPLWKGFSSATHINPAKYIVEYLIQKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B908</accession>
    <name>F4B908_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinate dehydrogenase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776946.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B908"/>
    <dbReference type="STRING" id="933801.Ahos_2160"/>
    <dbReference type="GeneID" id="10601663"/>
    <dbReference type="KEGG" id="aho:Ahos_2160"/>
    <dbReference type="eggNOG" id="arCOG00338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="OrthoDB" id="144689at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051278">
        <property type="entry name" value="HdrB/HdrD_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947:SF1">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="149"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="291" mass="32081" checksum="A484E00AB0B0AF30" modified="2011-06-28" version="1">MKVAYYPGCATHGLSKDVDIATKKVAEVLGLELVEVEDWNCCGGGFLDEYNEKAHVALNLRNLSTVERMGMDKMVTPCSVCLQSHRLAAYKYNENKDLRKEVDKKLKEANISYSGKATAEHIVWVLVRDVGLEKIKKAVKKPLTGLRVGAYYGCQMLRPEQIMGFEPSFNPHSMEDLIAATGATPVKFPMATACCGFPLMGSNPKVGLKLAYNVLNSAKSQNADILVHPCSLCHLQLDVTQLKVKNEFNLSWTLPAIYITQLLGLAFGFSAEELGISKLAQEVLRKKGVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B917</accession>
    <name>F4B917_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776955.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B917"/>
    <dbReference type="STRING" id="933801.Ahos_2169"/>
    <dbReference type="GeneID" id="10601672"/>
    <dbReference type="KEGG" id="aho:Ahos_2169"/>
    <dbReference type="eggNOG" id="arCOG05956">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170079_0_0_2"/>
    <dbReference type="OrthoDB" id="19360at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="12012" checksum="2EBBE5BE5572D935" modified="2011-06-28" version="1">MDSGISITAEKLIELTVKKASLISVKEDEIVRAVGYSSKDMAERVIDKVSFWLSYEQNHLLYCKLCNKGPFTKRGLFLHLTRVHRMEIKAMLEDELKREIKALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B923</accession>
    <name>F4B923_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor 1A</fullName>
            <shortName evidence="5">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">eif1a</name>
        <name evidence="10" type="ordered locus">Ahos_2175</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5 8">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776961.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B923"/>
    <dbReference type="STRING" id="933801.Ahos_2175"/>
    <dbReference type="GeneID" id="10601678"/>
    <dbReference type="KEGG" id="aho:Ahos_2175"/>
    <dbReference type="eggNOG" id="arCOG01179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="OrthoDB" id="2586at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003082">
        <property type="entry name" value="PRK04012.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003084">
        <property type="entry name" value="PRK04012.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003085">
        <property type="entry name" value="PRK04012.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE95046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="12097" checksum="D1D96CEF12FFF363" modified="2011-06-28" version="1">MPKKDPEAPVREVMKPAEGEVICVVKKMLGAEHIVVLCVDGKQRTARIPGRMRKKTWIKEGDVVLVAPWDFQPDKADIIHKYMHDEIKKLIDEGVIDKEVLDQLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B945</accession>
    <name>F4B945_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein AsnC/Lrp family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776983.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B945"/>
    <dbReference type="STRING" id="933801.Ahos_2197"/>
    <dbReference type="GeneID" id="10601700"/>
    <dbReference type="KEGG" id="aho:Ahos_2197"/>
    <dbReference type="eggNOG" id="arCOG01117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_1_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="76" mass="8433" checksum="184D07A8A45D9BB5" modified="2011-06-28" version="1">MGNVKAYILLITSIGKELDVVNDVKKLNGVKDATPVYGEYDVVVEVEAPDLNELNKTINQIRRNSSIIRTVTLISM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I7</accession>
    <name>F4B9I7_ACIHW</name>
    <protein>
        <submittedName>
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    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR40730:SF3">
        <property type="entry name" value="HTH CRO_C1-TYPE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="98" mass="11071" checksum="33826F726513130D" modified="2011-06-28" version="1">MIKLIIVERLYAQGLSQLQIATLMGISSAEVNYYLKGKRGNEDAKKKLEADEEFMDLVNSVVRRLVNSTDGEVINICPLCSLARKKLNKNDYSCPYDI</sequence>
</entry>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="933801.Ahos_2263"/>
    <dbReference type="GeneID" id="10601769"/>
    <dbReference type="KEGG" id="aho:Ahos_2263"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050276">
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    </dbReference>
    <dbReference type="Pfam" id="PF00583">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GNAT"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="145" mass="16401" checksum="63D41D4D33483634" modified="2011-06-28" version="1">MINALQIKKANFSDVEGIYNLYNSLSPDDLYMRFFSFKRLSEQDVVNLTKSSKVTLIAVIDSEIVGEISLYDDGEFSLVVKPEHRRKGIGTLLVKSIIEEAKKLGMTKVKFYTLPDNIPMIKIGKKLGFTLRFSSDEVFGILSLQ</sequence>
</entry>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="GeneID" id="10601774"/>
    <dbReference type="KEGG" id="aho:Ahos_2268"/>
    <dbReference type="eggNOG" id="arCOG00535">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="68" mass="7624" checksum="92DB948DC7EFBD26" modified="2011-06-28" version="1">MKVEVSLARENITKSLEIPDYYRVRDLLKYLGYTIQGTVVLRNGIPIIEDEKIKDGDKLTIVLTASGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K6</accession>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="KEGG" id="aho:Ahos_2281"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AEE95152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="161" mass="18593" checksum="B704F8DB58AE5850" modified="2011-06-28" version="1">MNCEDELKEAIILAWIGDREGVNKITKECIKELSSYRSAIKDITKIKEEVNREFEIPKKLREKGITHEDLLGLALLRLARKISLTSDLNPKNDGKLKYIIIDLGNKKILRGYCKECKGFHYTILKDNIGFAVEYDQIIYAEFLQGDEKSVMDVIKWEIIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N4</accession>
    <name>F4B9N4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase M48, Ste24p</fullName>
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    </protein>
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        <name evidence="9" type="ordered locus">Ahos_1066</name>
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    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
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        <text evidence="6">Binds 1 zinc ion per subunit.</text>
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    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775866.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N4"/>
    <dbReference type="STRING" id="933801.Ahos_1066"/>
    <dbReference type="GeneID" id="10600540"/>
    <dbReference type="KEGG" id="aho:Ahos_1066"/>
    <dbReference type="eggNOG" id="arCOG01333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529223_0_0_2"/>
    <dbReference type="OrthoDB" id="43570at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="8">
        <location>
            <begin position="45"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="179" mass="20298" checksum="E3B466FB2493004C" modified="2011-06-28" version="1">MQIIIFGLLSLLVINVVYFAVSIILVKKFKSIKYDFLGKQIELKVKEDARVNAFSVITGKILVTSASLNLPKDELDAMIAHEMGHIKLHHHLKMLILVNLLVLLFFYTAEFSLFLFIISGISIVLIQRFISRKFEIQADRYASQLVGKGSLMNLIMKYGENKSSIFSTHPPSSARIKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R4</accession>
    <name>F4B9R4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775896.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R4"/>
    <dbReference type="STRING" id="933801.Ahos_1096"/>
    <dbReference type="GeneID" id="10600570"/>
    <dbReference type="KEGG" id="aho:Ahos_1096"/>
    <dbReference type="eggNOG" id="arCOG00946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_2"/>
    <dbReference type="OrthoDB" id="5682at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF5">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="67"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="352" mass="40450" checksum="8C73956DB9A5449B" modified="2011-06-28" version="1">MSKEAVLYRKEGNRVRCLACARRCLIGEGQIGFCGVRSVRNGKLYLDVYGKVAAAHVDPIEKKPLVHFYPGSKVFSFSTYGCNWMCMYCQNYDLSQRRRPEGYEVSPEELVDMAIAYEAEGITYTYNEPAIFAEFAHDTGVIARKRGLFNTMVSNGYWTEELVDYVRDFLDAVTIDFKGNGEEKFMKRYTGASGPEPILETIRELHKNGIHVEITDLVIPQIGDDLKAAENLLKKIMDILGPDVPIHFLRFHPDYKLNYLPWTPVETLEKHYNLAKSLGFNYVYIGNVPGHPYESTYCPKCHNLVIGRDGFRITEWHLTEDMRCQYCGYKLPIVGKLSKHAFEEDRFEPVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S2</accession>
    <name>F4B9S2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775904.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S2"/>
    <dbReference type="STRING" id="933801.Ahos_1104"/>
    <dbReference type="GeneID" id="10600578"/>
    <dbReference type="KEGG" id="aho:Ahos_1104"/>
    <dbReference type="eggNOG" id="arCOG04318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099315_0_0_2"/>
    <dbReference type="OrthoDB" id="26985at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14820">
        <property type="entry name" value="TRAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011156">
        <property type="entry name" value="PRK14562.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="23032" checksum="C17EDA6ECC953F1F" modified="2011-06-28" version="1">MIEEIQSYLDGIEEKLTERFDNREKLLLLSRELIRVCGETISLSHRQKKEEALKKYEEAIQKAKEIEQIINQYPELLYGDVGTSFQELAEVSVVINMYFGGKLYLADELGIPDMYYVLGIADAVGEMRRAILEFLRKGDYENADKFFNYMETIYEELWKFEYPKSLIPGLRQKIDALRRILEETRHDLFLAKLGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U2</accession>
    <name>F4B9U2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="2">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_2287</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U2"/>
    <dbReference type="STRING" id="933801.Ahos_2287"/>
    <dbReference type="KEGG" id="aho:Ahos_2287"/>
    <dbReference type="eggNOG" id="arCOG00310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000007">
        <property type="entry name" value="Thioredoxin-dependent thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF4">
        <property type="entry name" value="AHPC_TSA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="12">
        <location>
            <begin position="9"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="11">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE95158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="154" mass="17352" checksum="51E299C9B1D4250B" modified="2011-06-28" version="1">MLRNYYTMLDVGQEAPDFEVESTLGKIKLSDYKGKKKVILYFYPKAFTAGCTRELQRFVQLYDEIVKLNAEVIGVSADSLSSAKKFAEKYGAKFPLIGDKEKKIINSYGVLNEKGTSAQRVTFVIDENGKITHVFKNLKKAEEHADKALEAIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U9</accession>
    <name>F4B9U9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Co/Zn/Cd efflux system component, CzcD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_2294</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777080.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U9"/>
    <dbReference type="STRING" id="933801.Ahos_2294"/>
    <dbReference type="GeneID" id="10601802"/>
    <dbReference type="KEGG" id="aho:Ahos_2294"/>
    <dbReference type="eggNOG" id="arCOG01477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_0_4_2"/>
    <dbReference type="OrthoDB" id="269083at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="3"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="10">
        <location>
            <begin position="192"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE95165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="30717" checksum="960B258DA05C0AB3" modified="2011-06-28" version="1">MRSIIGFWGVFVFLVITSILAKSAVLISEAIHVFLDALIVSLSFYFLKLVNKVNSYYTYGLHRLEVISSLLNVSIIILGSIIGVIISVIYLMMGIKDNPFYVLFASLISALILSFSHEHEEKENRVKESINVHIISDVLSYVLGALAGFLIILTGYYELDPIFSFIILGVMIIYNFRYFSTYLDIIMEKSPIDTKRIEDDLKSVFPKVHHIHVWTICDHYKVATLHIEEDPNVTLKELDYKREVAEKILSEKYGINHVTVQFEAKRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V0</accession>
    <name>F4B9V0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777081.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V0"/>
    <dbReference type="STRING" id="933801.Ahos_2295"/>
    <dbReference type="GeneID" id="10601803"/>
    <dbReference type="KEGG" id="aho:Ahos_2295"/>
    <dbReference type="eggNOG" id="arCOG01773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_14_1_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="37"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="202" mass="23221" checksum="246B8FEB23192EE3" modified="2011-06-28" version="1">MEIFNDPQGYLQWYKRNKMIYESELRAVKRLNLSNCLDIGAGPSVFHEAISGRIISLDISDAMLRLTNPTEDKIQADALYLPLRNKSVECTFISVTICFISDVEKLLQEVQRVTKKEFAVCIVPEDSSWGQYYTELGKKGHKYYSKAHFISKKELISLVQQYFEIKDTISTLRYSPFDEPKVEDPEEGLKGSYICVKAKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W7</accession>
    <name>F4B9W7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W7"/>
    <dbReference type="KEGG" id="aho:Ahos_2312"/>
    <dbReference type="eggNOG" id="arCOG09699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217604_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="241" mass="28109" checksum="E9E3B09E01B3141C" modified="2011-06-28" version="1">MMQQKLVTAIKLMRQIGPNIPLISKILKTEESSLKEQLLKIKNLSFTPVYSSSIIKINFDRLPIRKSIKSNLSFSLSLKDGLRIKEFINYSGIPEYYDEKYDKWKMSLDKRADGLKVEVKYSGYAQPLIFSSLRSFSSFNEILKGMYNHTVIESFIIDYGNRDAILDIFHNSKKVLECLELIPFIRFSILIEIDNNEELYITELSLPFTYLNYVSELLSELKPEIDIIFTLNKFPYISKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y7</accession>
    <name>F4B9Y7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777118.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y7"/>
    <dbReference type="STRING" id="933801.Ahos_2332"/>
    <dbReference type="GeneID" id="10601840"/>
    <dbReference type="KEGG" id="aho:Ahos_2332"/>
    <dbReference type="eggNOG" id="arCOG01213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237940_0_0_2"/>
    <dbReference type="OrthoDB" id="120822at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="NCBIfam" id="TIGR01487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="224" mass="25437" checksum="6BFA4C4B27F50021" modified="2011-06-28" version="1">MFLVASDYDRTLASEKNGFIISDEVKEKVNEFSKKYPFVVVTGRERKFIKVLAKGLNPTAWVLENGPLLLIGEKEIHLVDEDWFEIRESIVEKLEKLGLRYSIGKIIIYLDNVGNFSNKLRFDCARVEWNRNDAMIMPKNMDKGKGLLELVKILNFKGKIIAIGDSQNDIPLFKVADYKVAVANALDEVKKIADLVLNEENGKGVISLLEMIESGNLFKKIDIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z7</accession>
    <name>F4B9Z7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrilase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777128.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z7"/>
    <dbReference type="STRING" id="933801.Ahos_2342"/>
    <dbReference type="GeneID" id="10601850"/>
    <dbReference type="KEGG" id="aho:Ahos_2342"/>
    <dbReference type="eggNOG" id="arCOG00062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_3_6_2"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050345">
        <property type="entry name" value="Aliph_Amidase/BUP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674:SF2">
        <property type="entry name" value="BETA-UREIDOPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43674">
        <property type="entry name" value="NITRILASE C965.09-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="267" mass="30714" checksum="34D765E8200AEEF0" modified="2011-06-28" version="1">MDIELAQIRPKLGDVEYNLEEHLEILQTSSADCVIFPELSLTGYVLRDLVYEVFKEAEKAVTKIVDNSKCAIFGTIKEVRPGILRNSAVISIEGNYDYIYKFYLPTYGLFEERRYFQPGDPLSDVKIFKYKGINFGVVICEDAWHPEPIEALALKGADAVFIPASSPMRRLEENLLIENNWESLIKAHSLMYGIWTAFVNAVGSQEEEYFWGGSMVSSPQGTIKVKAKKFEEDRIFTKIDLEDVRKARFFSSFRDHNRDFHRLLSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA16</accession>
    <name>F4BA16_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="7">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartate transcarbamylase</fullName>
            <shortName evidence="7">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrB</name>
        <name evidence="11" type="ordered locus">Ahos_1129</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775929.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA16"/>
    <dbReference type="STRING" id="933801.Ahos_1129"/>
    <dbReference type="GeneID" id="10600603"/>
    <dbReference type="KEGG" id="aho:Ahos_1129"/>
    <dbReference type="eggNOG" id="arCOG00911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_1_2_2"/>
    <dbReference type="OrthoDB" id="7792at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="10">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="9">
        <location>
            <begin position="147"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="299" mass="34261" checksum="AC168D999439B5A2" modified="2011-06-28" version="1">MRDIISSLDFSKEDFLTIFSLADEIEKGKRLKLQEEKVVATAFFEPSTRTHLSFTTAALRLGAKTMGFSSTEGISEAKGENFADTIRMLENYSDCIIIRHKFDGAAKFASEISNKPIINAGDGKHEHPTQTLIDIYTVYKSFGSPDNLTYGILGDLKYARTVNSLLRGLTRFKPRFVYLISPEPLKARKEILSELNYPYKEIDSPSEVISEIDVLYVTRIQKERFPDEMEYEKVKESYTVDENLVNQMKKDSIILHPLPRVNEIDRRIDSKPQAKYFYQASLGVPIRMSLLYKILSGEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA60</accession>
    <name>F4BA60_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">A-type ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">atpD</name>
        <name evidence="5" type="ordered locus">Ahos_1174</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775973.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA60"/>
    <dbReference type="STRING" id="933801.Ahos_1174"/>
    <dbReference type="GeneID" id="10600648"/>
    <dbReference type="KEGG" id="aho:Ahos_1174"/>
    <dbReference type="eggNOG" id="arCOG04101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069688_2_2_2"/>
    <dbReference type="OrthoDB" id="117390at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001544">
        <property type="entry name" value="PRK00373.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="213" mass="24548" checksum="79029E0C8664D6E0" modified="2011-06-28" version="1">MSSNKVLPTKLNLIRFKNELKLVSTIKRLLESKREVLLIYLRTYAAEYEKLYNEVNEALKKTYNSYMQAVVDEGITTIEKIADSQSHSLSVSSSLKVIFGVKIPVIQLNEGSIPEKPFSEVETSPYLSEAYDEMREALVKVVQLVELESTIRSLAAELRRTQRLINAIDTSILPFYRGSVKYIKSVLDDKAREEFVRLKITRRILERRRQSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA80</accession>
    <name>F4BA80_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH-ubiquinone oxidoreductase subunit H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1194</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775993.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA80"/>
    <dbReference type="STRING" id="933801.Ahos_1194"/>
    <dbReference type="GeneID" id="10600670"/>
    <dbReference type="KEGG" id="aho:Ahos_1194"/>
    <dbReference type="eggNOG" id="arCOG01546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_2_2"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004741">
        <property type="entry name" value="PRK06076.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="NADH DEHYDROGENASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432:SF3">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="353" mass="39341" checksum="733ABF73F37A0108" modified="2011-06-28" version="1">MIGDLLSLIRFYFFYPSFFVVIIFPGLLFTGILLLTTIWFERKAAARVQMRVGPYYASKKLGGYLQLIADALKFVFSEVIIPAGVNETLFVIAPILLLLVSILPFAVIPVSVIPQSGSVFSIYYHDFYDPNVGYGVLAGIFTCYNLLLILALESIYPIFVIFLAWVTNNRFAIVGAVRETFLSVSYDALILIATFSVAIEYHTLDLAVIVQKGIPGVIVNPIAAFIFIVGMLIGSSRFPFDIVEAETELVIGPYTEYSGFLFVLTMAGSYVGNFIYSLVFADLFLGGWYPFSGLPGAAFMTFKAVLLLFFSVFLRSVYGRYRIDQALRGSWKYLFPLSLISLITGTLVGYLWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M8</accession>
    <name>F4B3M8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774834.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M8"/>
    <dbReference type="STRING" id="933801.Ahos_0022"/>
    <dbReference type="GeneID" id="10599462"/>
    <dbReference type="KEGG" id="aho:Ahos_0022"/>
    <dbReference type="eggNOG" id="arCOG07715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269847_0_0_2"/>
    <dbReference type="OrthoDB" id="375313at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="23871" checksum="950F8EBE87C0DF3A" modified="2011-06-28" version="1">MIKKAVPLILLFLISTTTILEASSTYKINILLQGKGISAIVEYCNHLELITTNKTLYLPNASVTITAYETIIGYNISINGKNISSLTFNPANLSEIIIKAFPIYVWINIKINGTGKVIINFQNGSKEIVNQSTKLKVLYGSLLTLNAKGTNGESFISWNNNYTYPTMWLIASNNTCVIVNFGKAKSSTFSSVNFLGIGLLVILGGIYIYIKRKQSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N0</accession>
    <name>F4B3N0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved proviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774836.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N0"/>
    <dbReference type="STRING" id="933801.Ahos_0024"/>
    <dbReference type="GeneID" id="10599464"/>
    <dbReference type="KEGG" id="aho:Ahos_0024"/>
    <dbReference type="eggNOG" id="arCOG00995">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536567_0_0_2"/>
    <dbReference type="OrthoDB" id="115061at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10630">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUF5591" evidence="2">
        <location>
            <begin position="25"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE92919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="20422" checksum="9E1666DC661098D9" modified="2011-06-28" version="1">MQCPPLSGPPIIKKDGIDLFKNPIVRKWYDFFLENWQSKKEVAFLLPCTSVKPYNRSPTHKLAYSIAKNKDYIQFYSVSEPMLLVPREYEECYPFNSYDYPPSMMTEEEKNEFVDLLSIMLRKISFQHKRIVAVLPKHHYNIVSRASEKVGIKIEIHPYGRLAFKTISDVLKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W7</accession>
    <name>F4B3W7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="6">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1271</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776070.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W7"/>
    <dbReference type="STRING" id="933801.Ahos_1271"/>
    <dbReference type="GeneID" id="10600752"/>
    <dbReference type="KEGG" id="aho:Ahos_1271"/>
    <dbReference type="eggNOG" id="arCOG05705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="2"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="203" mass="23008" checksum="B3067D3EE311D3A2" modified="2011-06-28" version="1">MTIEAKVVKKLGNFYLNAQIKEQGIVCITGKNGSGKTTFLRSLAGFLNIDEGYVKINNVDVTKLSPEKRGTVIITPDSYIPGLKVKSHLVWGAKIRKIKYDINELEKYKDLLDGISLDEKVDKLSLGQRERVSILTAILAKPKVILIDEAFSNINDKEYFVERTIEIMRKNSIELIFTTQDINDSQFANSHLRMEDGKLLKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z2</accession>
    <name>F4B3Z2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774873.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z2"/>
    <dbReference type="STRING" id="933801.Ahos_0061"/>
    <dbReference type="GeneID" id="25395595"/>
    <dbReference type="KEGG" id="aho:Ahos_0061"/>
    <dbReference type="eggNOG" id="arCOG01417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053640_0_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="239"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE92956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="419" mass="48856" checksum="5D832BCF1FCBCD6B" modified="2011-06-28" version="1">MRILLHSYLLEAGSGGVKRNREVIKYWKKYVDEIIYVPLLGDMRRASEDDEFRKKMYNEIKSLGLEIPEKVSEVIDSEVIKRVPINMSKLKFDLYSNTTQYKINSEISKIMGKIDADIYYAQQEFPQMVDFLTKIAKNRNVGALIQNFLGSLTVDFSHFYRAYSSMGLSGIMYAFYVTFLRSYPRRRKWVELINSGKVKFAFSVSEPLAEIYPFMRKIYTGVLKPGNAFDKKLLDFRDKGKEDYSVFYARLSPSKGVLELLKIWRKIDENKKLIVMGKFSDKKVEKLFMKNKPKNVEYLGFVSQDKLIDVVSKAKVLVYPSHFDSFSLVILESLALGTPVIAYKIPGIRSIYEGLNAVKLVKEGDLAQFRQEIRKIYDMPTKEYEDLINDKKLWEFLYEHSSWEKVALNELEELKKVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z6</accession>
    <name>F4B3Z6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall biosynthesis glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z6"/>
    <dbReference type="STRING" id="933801.Ahos_0065"/>
    <dbReference type="KEGG" id="aho:Ahos_0065"/>
    <dbReference type="eggNOG" id="arCOG01397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055387_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="21"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE92960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="341" mass="39822" checksum="118A613CF37285E1" modified="2011-06-28" version="1">MKNAYFFNNIILLVMMNNYISVIVIAHDRKKYIIDAINSVLNQKLDKNYYEIIVVKNFKDDKIDKFIENNGITKNYITEEKGVGKKAAIGIDNSSGDIICFLDDDDMFTENKLAIVLDAFSKNNGLIYYHNNQIKIDENGKILDNPQYNGKMIISDITEKSIAFIFRNFGEFNSSSICIRKETIDTNMLRKLDRAVDYFYLLSALKTRREIMLDFSPLTYYRIHKSAMHFFSNTFEEIISSACNYYHEQSKAKEAIYEYFKNDSLLAQVTKADVYTFTAISAILCNKSKFLPFKNSIRILDESIYFPSFRLKLFLASIINLISNKLARDIYVKMYLRKFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B403</accession>
    <name>F4B403_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdopterin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0074</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774884.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B403"/>
    <dbReference type="STRING" id="933801.Ahos_0074"/>
    <dbReference type="GeneID" id="10599514"/>
    <dbReference type="KEGG" id="aho:Ahos_0074"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_2"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2070.10">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050612">
        <property type="entry name" value="Prok_Mopterin_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742:SF6">
        <property type="entry name" value="OXIDOREDUCTASE YYAE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742">
        <property type="entry name" value="TRIMETHYLAMINE-N-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin oxidoreductase" evidence="3">
        <location>
            <begin position="159"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE92967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="582" mass="66365" checksum="1C09CCFC4E1C4ECF" modified="2011-06-28" version="1">MIACTRDCYDTCIFDSNYKPLDIFPINDFTCSRGIADLKRNSINRIKSAYYEGKEITVEEGIRILAKRIREINPEEILHIDYDGNQGLLTWYYPARLWNYLGSSSTDYSICSAEGHEAIKAHYGSSFGALPEDFVKFSSVVFWGSEASISFIHGWKILKDKYKVTIDVRKSETAKRSDKYYIIKPGSDVYLAIGILKKLVEKGRIRNAELQDKVASFNYSLIEEATSLKEKDIEDLADFYFNEKPLTIIGFALGRSLNGGYAIGVISLIPGLLGIENGFFYSNSQGWGIDFDYLRGTHLYKPRKIINMGEVGDRINEFKIIFVWNSNPLHSLPGGDKIIEAVNDGRVFLVVHDPFWSETAKLANLVFPGLTFLEKEDVVYSYWHNYLVYNEPIVNNPLGITEVNLMHKIARELEISEDFIFEDPWKAVEVSLRNTGISVEELKRRKVVKVSPKVTRKFSIEPFPKPDDLEFPQGEFLVYSSHPNYTNSQFKEIYGERKPIVFNSKFEGEGILYTEKGQVRVIFKKSEDLPSGVYFLFKSSLFDLDGKPINSIIDGKKGKFGGTPLLNIKVQAKPVKLFTLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B430</accession>
    <name>F4B430_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0101</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774911.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B430"/>
    <dbReference type="STRING" id="933801.Ahos_0101"/>
    <dbReference type="GeneID" id="10599541"/>
    <dbReference type="KEGG" id="aho:Ahos_0101"/>
    <dbReference type="eggNOG" id="arCOG00561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032023_0_0_2"/>
    <dbReference type="OrthoDB" id="85618at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050297">
        <property type="entry name" value="LipidA_mod_glycosyltrf_83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908">
        <property type="entry name" value="MANNOSYLTRANSFERASE YKCB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33908:SF11">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase RgtA/B/C/D-like" evidence="9">
        <location>
            <begin position="78"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE92994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="388" mass="44325" checksum="45E73D6ADE344CDF" modified="2011-06-28" version="1">MSRKYVDILFYSLLAVIVATYTYFTIKTFLPINAYIGDEVWYPTAAYNMLKLIFHISPPMYFPYPDKPGIETFINVCHPPLAKYIMDIFIIVMGYYPLSWRLPSWIIGDLILITGFFFTRDILGKDLIGNLGGIFTSIILASCPNLWVLHGIAMLDIYVAFFSFLSLYFLLKNKLLLSAIFLGLAIASKEPAVMLIFPALYYIGEIERKPIKRAYLGLGIPAFVYLIISLPIIIYEGGPLAWLHAKIANTVVWDISNGHISLDAVTQISEPWDWFLNIHPFNMGHGLYANVNPIIMILWAVTTPLAFVMKNLKMIMTTMFAWSEWLGFVLVYVLGNHTQFSFYVTDFAPFVDSYVVISLFMIARKFYIKKGEEKNIEENKQNYSGGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B446</accession>
    <name>F4B446_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein DUF95</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774927.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B446"/>
    <dbReference type="STRING" id="933801.Ahos_0117"/>
    <dbReference type="GeneID" id="10599557"/>
    <dbReference type="KEGG" id="aho:Ahos_0117"/>
    <dbReference type="eggNOG" id="arCOG01995">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357841_0_0_2"/>
    <dbReference type="OrthoDB" id="42180at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="201" mass="22690" checksum="4726170D49953EB0" modified="2011-06-28" version="1">MRRQTLVFFILFIIELLIFIGTSALPVNQPELASKFQSERSSIVSLPYPLEALSIFTHNYEVALAEFIPALGVGIMGYSIGSTGYVLSAVSNEQGVPGWIPAIFLLTLPHSWLELPSYAFAATAGLFLLIDRNWKRFLYMIGFVGLELFFAASVEAGEIVLENVNVIYSYLFWIPAALLFYVLYEVYEYIMDVTEKPKVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B448</accession>
    <name>F4B448_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TrkA-N domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774929.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B448"/>
    <dbReference type="STRING" id="933801.Ahos_0119"/>
    <dbReference type="GeneID" id="10599559"/>
    <dbReference type="KEGG" id="aho:Ahos_0119"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822869_0_0_2"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF9">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN YUGO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="127"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="338" mass="37478" checksum="E111D2283ED34D21" modified="2011-06-28" version="1">MGRTRIWNRYLIPLVENFVAPYSVLRKILPQILLLGLAVYINAWVFMVYQHLDFISAIYAGVNVVTTVGLYAPNISQMTDTEKIVLIITIIFAVGLYTSIIQSIVSTVINRSTWQDAKARWRGSHMKGHTVIVGEGEIITSAVRRLERLGVDYIVLTPSKDIANKIRNDRVIIGDPKDDKNLLSAGILEAKNAIVSMQNDMDTLLITLKIQKINPPLQVISVVKDSNMVDVFKTAGADLVIPNDDIVGRITAAAAISNNVAGVIFHDRSENLIIGSFEIKKEIKIKDLPPGILPLAIIEEDGKLNPYFEKDTLLKKGEKLVVLGDPNLFKKVKEVLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A1</accession>
    <name>F4B4A1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">aminomethyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1326</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776124.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A1"/>
    <dbReference type="STRING" id="933801.Ahos_1326"/>
    <dbReference type="GeneID" id="10600812"/>
    <dbReference type="KEGG" id="aho:Ahos_1326"/>
    <dbReference type="eggNOG" id="arCOG00756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_2_2"/>
    <dbReference type="OrthoDB" id="2001at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF2">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="9">
        <location>
            <begin position="268"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="346" mass="39714" checksum="DCB6A19703EA4258" modified="2011-06-28" version="1">MFKTPLSAVEEKIGANFGEFAGWEMPMDYSSYQDEHMAVRQSAAFFDLSHMGRIRIKGKKEELDKIVTRSLSTENFRMIGPSAMLNEKGGFIDDIMIYKVSDTEFLMVTNAINREKDINWIKRNSSLEVEDLTFDYVMLAIQGRRVWELLGYKTDLQPLQFVLNSNFLGEDVFLLSRSGWTGEDGLEVWAKVEIAERIISKLMEKGIKPAGLITRDSLRQEMGFVLYGEDIDENTTPVEARYWVFDLDKNFIGKEKVEEQLKNGVKRIRVGFKLQKGIRAIPRHGYRIRILETEVGEVTSSTYSPYLGRVIGMGYINSSNAFFGFHANIEIRGKDYRAKISDFPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G9</accession>
    <name>F4B4G9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774975.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G9"/>
    <dbReference type="STRING" id="933801.Ahos_0165"/>
    <dbReference type="GeneID" id="10599605"/>
    <dbReference type="KEGG" id="aho:Ahos_0165"/>
    <dbReference type="eggNOG" id="arCOG05883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168616_0_0_2"/>
    <dbReference type="OrthoDB" id="35384at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="235" mass="27080" checksum="6C54B78729663B24" modified="2011-06-28" version="1">MKCSTEEIKEMRGFQFFVNNCDKDDLYKRISDIKSLSDSSGKTYILRLAESNYFRFELLFIPNTVTLISVATARGTNNINLRDFESLDDLISLSCCVSTNEQLREASKNFLNERTNEAEIVDLFSQKEEFEVNTDCSKVKWNEIKFPETVEGKWNTTELATVGKLSLKALAGQIKFLAISTERPQNSIKFTNFERFSSLCCFLNLVKKKLDECPAIVEAIKPFLTQEKKRRSRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I1</accession>
    <name>F4B4I1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774987.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I1"/>
    <dbReference type="GeneID" id="10599618"/>
    <dbReference type="KEGG" id="aho:Ahos_0178"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2216903_0_0_2"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="11821" checksum="09CD67344CFC7FD3" modified="2011-06-28" version="1">MKTLLEQKGEQPLAGRNPVKITLKPWDYETAKEYLKLGLEECGVAHSDKEIQEALSILGTLVGWLNFYGVNRCVKPHGDALKESLEEAISIALSELKKNLQIRSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T2</accession>
    <name>F4B4T2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775014.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T2"/>
    <dbReference type="STRING" id="933801.Ahos_0205"/>
    <dbReference type="GeneID" id="10599645"/>
    <dbReference type="KEGG" id="aho:Ahos_0205"/>
    <dbReference type="eggNOG" id="arCOG02219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118482_3_1_2"/>
    <dbReference type="OrthoDB" id="38049at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09881">
        <property type="entry name" value="PIN_VapC4-5_FitB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000103">
        <property type="entry name" value="PilT protein domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051749">
        <property type="entry name" value="PINc/VapC_TA_RNase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42740">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42740:SF1">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14545" checksum="35D254AE02D1C9D1" modified="2011-06-28" version="1">MANKGICLDTDILIDAFKSDVRKIIGYYTTCINLYEFLRGLAFIGKDLDEFKLWIEINLNVLCIDNNSLKVASKIYAELRKKGEIIEDPDLLIASICIANNLPLITHNKKHFKRLEKFGLKVVDKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W0</accession>
    <name>F4B4W0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DMSO reductase family type II enzyme chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W0"/>
    <dbReference type="STRING" id="933801.Ahos_0233"/>
    <dbReference type="KEGG" id="aho:Ahos_0233"/>
    <dbReference type="eggNOG" id="arCOG01506">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340670_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3480.10">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="TorD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="204" mass="23932" checksum="A06A421439BEB849" modified="2011-06-28" version="1">MIKMIETSIKDTLSFFKYSLFDFLAQAFHYPYKLKDIKDLQNKARELEDLVKSFCDTQFVESFENFKKYLDSINNVDKLTEVEIQFVELDKPYNVHLSLYETINRFGYYDLVTSGKLINLYTTVGIKPKEEPDLLSTELGFLAFLYFGKAIGQDTSQIISTFLEEHINKWVPKLIENLKQSKYEYFVVLSELLDKSLRCIEGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X6</accession>
    <name>F4B4X6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775058.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4X6"/>
    <dbReference type="GeneID" id="10599689"/>
    <dbReference type="KEGG" id="aho:Ahos_0249"/>
    <dbReference type="eggNOG" id="arCOG07220">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2379212_0_0_2"/>
    <dbReference type="OrthoDB" id="375388at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10834" checksum="A922856393CC624D" modified="2011-06-28" version="1">MAKRFDVKIEDDRLIVYNLHIEPLYIKEIAISYEVEVITPTGEKGIKKVKQTIPIEKKVVKVLDVPIVSREISEVKVIYIAGNEKAEEIHHPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B501</accession>
    <name>F4B501_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775082.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B501"/>
    <dbReference type="STRING" id="933801.Ahos_0275"/>
    <dbReference type="GeneID" id="10599715"/>
    <dbReference type="KEGG" id="aho:Ahos_0275"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000009">
        <property type="entry name" value="Aldehyde dehydrogenase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053507">
        <property type="entry name" value="NADP-Glyceraldehyde_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040792">
        <property type="entry name" value="glyc_ald_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="13"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="495" mass="55402" checksum="6BF151F9C3A95E10" modified="2011-06-28" version="1">MEKIQELFINGEWVKPSSEEYLNKYNPSTGELYGKFAAASKEDVDRAIDAAYDAQKKWEKLTSVERSKYLYRLIDLIRQDRESLENLLMDEVGKPRKEAVQEVDGVLDQLQYYAEFARKITGDVVEGTKPERVIFQYKVPYGVVLAITPWNFPAAMIARKIGPALITGNTVVLKPSSDTPFVAGWIVEKVRQAGFPPGTVNLITGKGSVIGDYMTSHKKISVITLTGESRTGVQVMKSASSIMAKLILELGGKAPFIVWKDADLELAAKVLMWAKYWNAGQSCIAAERLYVHEDVYDKFLNLFIEKTKTLKIGEPRTSDMGPLINDQQLKKVSGFVEEAVNQGAKILYGGGKPQLPERYSKGFFYMPTILTDVKQDMRIFREEIFGPVIGAMKVGDDFDEVIELANDSDYGLASYLFTKDVSLAMKAAREIKFGELYINMPGPEATQGYHTGFRMSGQAGENSRYGVEEYVKVKNVYIDYSKDPTAGEVIPPYTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B518</accession>
    <name>F4B518_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776235.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B518"/>
    <dbReference type="GeneID" id="10600933"/>
    <dbReference type="KEGG" id="aho:Ahos_1437"/>
    <dbReference type="eggNOG" id="arCOG07720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_592650_0_0_2"/>
    <dbReference type="OrthoDB" id="44212at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="461" mass="50004" checksum="50FB95FB09994A65" modified="2011-06-28" version="1">MSTGPVPIPIPEGAVVAIAGIIGAALSYGSIRDTATCTAMQMKEKGFSYEFYPALATSVRVSKATKNIFQVIRHGIIIRTQEGHYYYVGGKSKYWVSDRSFQAYQGGTNFYNNTNGLVTKIRDNESNIVVVRMRANRISSAWLQPNPPEGCNTPFVGWFLDALESAAAGAIMMNYIPYFTSRSFSDIEVPGELITVSGGHYSADSLSGILRADSGLPPFPYMVIATISKQATFKVPPAVQRGSAYVLFPASVMDGLCKFFLVGSSEKYCSKLVSNTSYNEALIGAPVFMSFSCTSGCKSVGLIGLVFDGNMLNVGGYSFGDLLIVEPPPYPYTDAGMLAYADELGVKDALDLSIRGVENAEKAISSIVSAYGISGVIASAIISYIIWTDSVDEMVNNAKPYVEKAKNAVERVREELIKTRNYRLLSYVDECVAQQDLDLDENDIYQGALGCVFSNIENVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B536</accession>
    <name>F4B536_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776253.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B536"/>
    <dbReference type="GeneID" id="10600951"/>
    <dbReference type="KEGG" id="aho:Ahos_1455"/>
    <dbReference type="eggNOG" id="arCOG05573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2581176_0_0_2"/>
    <dbReference type="OrthoDB" id="375862at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8943" checksum="6858534B98D8C708" modified="2011-06-28" version="1">MDAKDKVMSSEIKSKIVLTLKKEGKLSFKDIKDLVGISTDTLKLQLADLIADGVIKKCKGKYELTEIGEEIGELLLKRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B545</accession>
    <name>F4B545_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776262.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B545"/>
    <dbReference type="STRING" id="933801.Ahos_1464"/>
    <dbReference type="GeneID" id="10600960"/>
    <dbReference type="KEGG" id="aho:Ahos_1464"/>
    <dbReference type="eggNOG" id="arCOG04060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077869_0_0_2"/>
    <dbReference type="OrthoDB" id="371772at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="238" mass="26762" checksum="8625268A526CF8CC" modified="2011-06-28" version="1">MASDYVIELIAKRIAGDIVWSSNIGLSMKKWREMFGISQSELARVLGISQTVIADYERGRRQPGSAFVKKFVQGLIEIDERRGFKVISELSKSFTLNFPFIMDMRDFETPICFDELTIAVDGIPLNSTINLKKIYGYVVVDSLTAITALSGMEFYQFLSLVFNRVIVFTKVTSGRSPIIALKISPVKPDVVVLHKPLKMDPLSIDLADKEGINIIISTKRNEEELIKSLRTLVHSKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B568</accession>
    <name>F4B568_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776285.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B568"/>
    <dbReference type="STRING" id="933801.Ahos_1487"/>
    <dbReference type="GeneID" id="10600987"/>
    <dbReference type="KEGG" id="aho:Ahos_1487"/>
    <dbReference type="eggNOG" id="arCOG10436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493003_0_0_2"/>
    <dbReference type="OrthoDB" id="34583at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="20896" checksum="15414AD1708C8C4E" modified="2011-06-28" version="1">MIKFAKRDNRGFFNDVESAIDIGRIHISPFIADELYIYIEDKDLLMNISYFDLIEILNSTRMYKVDMIKRNTRYDKIGIIINQDYLGGINVCTIIDWGTQKIVSSVNNEKIRLDHGPDCEYNDCVYIALFNFFNELYYLKIRITETDIQPSLFKVDLLNFVNEIVFYELRQKFKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B588</accession>
    <name>F4B588_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B588"/>
    <dbReference type="STRING" id="933801.Ahos_0291"/>
    <dbReference type="KEGG" id="aho:Ahos_0291"/>
    <dbReference type="eggNOG" id="arCOG03285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441225_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF13">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="590"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="618" mass="70338" checksum="8E40DDF93B1CC6D8" modified="2011-06-28" version="1">MVVKVIILLFTLLLSFPGYFVIGTSSESCYSNPYYPSYLLLSNWRNISVWIPTGIQVINRELNGFPPYNASIRNIFLGGIGILNLTVCLGINTKKAYLQLNNTVLLECNNSICIILPPYTSYLLLILKVKSPFNITIKSNSSWLAQDNEFRNNISWLISNGSVIIKRTNLILSFQKTGEYYAEISLLVPPYENISHLLLLNDNSVNSWISKSVIPKYLPKSLFREYYLSLLLIKDDQNPYLGDFAASPSPIYLYSWVRDSSFAALALQESGHYYSALKYWLWMANATSMGNGTWYTRYNFYTGKPCIGFGIPELDSLGLFEIGVYNFYILTHNASFLDEVKPRLCEIIKYQIYEILKSKFHLVPEDLSVWEDRLAYHFWTQSFNDLGLYDMEKLGFNVSYEEKLLNESILKYFWKNGYFASALGESVLYEENGEVEVLCPLPPAIDSSTLLPLDMGYLPLDSNYTNQDFNSVVSNLTVNGGLSRFPDDLYHYSESLYDSCEPSPPWVITTLFEALYYAEKGETNSSIKLLCWAYNHSRQGLLPEAVAPVKGYPLPTTSPLTWSSAMFIIVSLSLKPQTSTTTNKECPKEFILSSIIILIILVISIIIYRKIILHTGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B597</accession>
    <name>F4B597_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B597"/>
    <dbReference type="STRING" id="933801.Ahos_0300"/>
    <dbReference type="KEGG" id="aho:Ahos_0300"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053400">
        <property type="entry name" value="2-oxoacid_Fdx_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041170">
        <property type="entry name" value="Oxoac_fdxalpha_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF16">
        <property type="entry name" value="PYRUVATE FLAVODOXIN_FERREDOXIN OXIDOREDUCTASE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="7">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="226"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="604" mass="67310" checksum="DB1E53F0CEF60C87" modified="2011-06-28" version="1">MIGGAQGTGVDTSANIFGNAIARAGYYIYGNREYYSNIKGRHSYFNLTISDEKARSISHKVDILASFDAETVFQHYQDVQKVIIYNKGVLNVEKDRIQSIEPETAERIPFKTVKDVIDYLESKGVTTIGIDYDEILKKVAEEMKLPLSVIDRARNTIAISASYDLLGLRKDYLYEALKRTFKQETFVKLNLSAADKVKLSPIFNLKELPQKKRRVQIDGNTAVAIGKIYAGIGFQSYYPITPASDESTFIEAHQEVLYVDPVTGDKRKRTIVVVQAEDELAAVNMASGAALTGVRAATATSGPGFSLMTEGIGWAGMNEVPIVISYYMRGGPSTGQPTRTSQADLLFAMHVSHGEFPRIVIASGDHVEALKDAIWAFNLAEKYQTPVIHLVEKALANAYSIVDLDELGGDIEIERSTIGDDNERFKFTDSGISPRLLLGESTVYYTGDEHNTFGHISEDPVNRVKMYEKRMRKLETADKEIPEEKRYNVYGDLDSKYAIVTWGSPKGAVLDAVEELDQKFAVIQLRMFNPFPKSVKKLLEDKDLIIDIEGNYEAQAAILLKQYGVEVTNYILKWNGRPMGRDEVRDGILSAIKGEKRVVLNAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A7</accession>
    <name>F4B5A7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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                <person name="Wang S.Y."/>
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                <person name="Liu C."/>
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                <person name="Prangishvili D."/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="181" mass="19530" checksum="2C29ABA614FF7C5C" modified="2011-06-28" version="1">MMFLQDALIVTLLGLTHGLDPDHIATARILKNSVKILKFSLFHSLGFVILAIPLTIILIITSINSVYLNIIGNLIGIGVALILLASALLGKEFEIEPKEMGLLQGSLVVTPSKIITVVLAISLDNIIYSTAIVGIFILSSTLSIFALSSLNMIPRKFDKPINVAISLLTIIFLGFSTFEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M1</accession>
    <name>F4B5M1_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776360.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M1"/>
    <dbReference type="STRING" id="933801.Ahos_1562"/>
    <dbReference type="GeneID" id="10601063"/>
    <dbReference type="KEGG" id="aho:Ahos_1562"/>
    <dbReference type="eggNOG" id="arCOG10215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115254_0_0_2"/>
    <dbReference type="OrthoDB" id="44231at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="20910" checksum="624BAC5834156CC5" modified="2011-06-28" version="1">MTRINKKVLLTLVFLSALIPVVAADVIYYYQGSVKVCKTAMPIELQVGPNGDQTGYITTTVGNAPRGYCFTATIYITNSTYDYFYHFLTIKACKPGNLFVTDVSTSTGTNIKCAWLIIQCASNGNTIGEFKIISTNAANQCTTTISLTSGNSYCVSLLVEPNNPLPNSGTCLGTITVYLGYNVVNGGGAISLPPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N5</accession>
    <name>F4B5N5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated protein, Csx1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N5"/>
    <dbReference type="STRING" id="933801.Ahos_0359"/>
    <dbReference type="GeneID" id="10599800"/>
    <dbReference type="KEGG" id="aho:Ahos_0359"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_695624_0_0_2"/>
    <dbReference type="OrthoDB" id="102285at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09728">
        <property type="entry name" value="Csx1_III-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10640">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3740.10">
        <property type="entry name" value="SSO1389-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027419">
        <property type="entry name" value="CRISPR-assoc_Csx1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052875">
        <property type="entry name" value="CRISPR_assoc_ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010171">
        <property type="entry name" value="CRISPR_Csx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like_HEPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053857">
        <property type="entry name" value="Csx1_CARF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01897">
        <property type="entry name" value="cas_MJ1666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169:SF1">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37169">
        <property type="entry name" value="CRISPR SYSTEM ENDORIBONUCLEASE CSX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22230">
        <property type="entry name" value="Csx1_CARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160980">
        <property type="entry name" value="SSO1389-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="CRISPR system endoribonuclease Csx1-like HEPN" evidence="1">
        <location>
            <begin position="336"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="417" mass="46960" checksum="E7C8344282E350F5" modified="2011-06-28" version="1">MKILFAPIGDPSGYVNVEYLVNGEGPYNTNASFIAISQALKIDKLVVYAGLSLCDTNCNDYKCCKDSVTQKVAQKLGNEFDLLIAPNIYGTRFIQKDRKNTLYFNFIYFNSLKILEDNKPDEIYIDITHGINYMPLLATDAIRLATYTYIVENNKNGVNLTIYNSEPVTKGNQGPYAIDDIYSEKITVRQALSSILSPFLSSESKNVIKNKVFKVIRNKANSENVNTSSCNADFIYHIVNALSAGIFLYLILKKDEINSCLNVVENILGKLDFNNFAVQLNFENGKVIYDENIPIEISYIHSLLKVSNHIAQGSNKISEIRRMAENYSTSDSVKYVILNEISRIEEKKNEIPNSPTLYAKIRGTDSPKICLPEKRNLIAHGGFEENVTFLWKCGEDICVSYTDGKCDCIENVEKHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N6</accession>
    <name>F4B5N6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775167.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N6"/>
    <dbReference type="STRING" id="933801.Ahos_0360"/>
    <dbReference type="GeneID" id="10599801"/>
    <dbReference type="KEGG" id="aho:Ahos_0360"/>
    <dbReference type="eggNOG" id="arCOG13098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465017_0_0_2"/>
    <dbReference type="OrthoDB" id="44224at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="184" mass="21462" checksum="846773703B7596C1" modified="2011-06-28" version="1">MNYVELLNNLLEHAGIEADISPFYISLIFLHELTHHVIEDWRTSSGTFSYLQEDERLCEYTAFTLTQAILGNLLRNQAFQTFVHEDHIHIFEGMANEAIIGFPVPGFFIPLNMDLPPQHNVQELLSLIYYYWNRDKDPLYKPLVLAWVNEMDLVRFLSQNLTVGLDNAWNKKGLPSVYTKVRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q5</accession>
    <name>F4B5Q5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CRISPR-associated protein, Cas6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775186.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Q5"/>
    <dbReference type="GeneID" id="10599820"/>
    <dbReference type="KEGG" id="aho:Ahos_0379"/>
    <dbReference type="eggNOG" id="arCOG01439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029036_0_0_2"/>
    <dbReference type="OrthoDB" id="42729at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041165">
        <property type="entry name" value="Cas6_N_arch"/>
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        <location>
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            <begin position="132"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="30306" checksum="3F5E0C2C1FC5AE46" modified="2011-06-28" version="1">MIFTFTFEVKPDRDAIIPPFTSKLSRSIFLDFSPTYSKFIEAEEPYKPFRITVLKDGNTPVYSKGNGMVTLTGGKIYNFSVTTTLQKVVEEVVRTTSIDKEIFNAKFHVELVNMEMKEELGLEDSRLYKVLFNTPTLLQPPRPKFKRKENRFILFPYVPSFFYSLSSHWNKFMSEKIVGVTGSKTLYYFREVDYNIRPVTTYYGKTPTRGFIGWVLFELRAKRNSKIRENVRKLLDYANYFGVGKSRSIGFGEVKVLKIE</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
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    <dbReference type="eggNOG" id="arCOG07349">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911277_0_0_2"/>
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    </evidence>
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    </evidence>
    <sequence length="61" mass="7394" checksum="8A0D9328C3332D99" modified="2011-06-28" version="1">MQLLLEGREIEIVKANEDYAEKFYEYLQLLKDDPENYTIIRYDDVTLEDVKKIRLSYVFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V5</accession>
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        <lineage>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <location>
            <begin position="46"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93320.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="102" mass="11843" checksum="C1E0D3BF58BCE284" modified="2011-06-28" version="1">MPIEELSKVLEEIRKKAYDTKDAVLKDTTRFYTTLHNTINSEIAKAKKEGKKIDDIQKEFEDLLKKIDGLREKQKSMSTKDLRNALVSYTQKAEKLIKKIKG</sequence>
</entry>
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    <accession>F4B5X6</accession>
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        <submittedName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1593"/>
    <dbReference type="HOGENOM" id="CLU_2339985_0_0_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94474.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="97" mass="11573" checksum="B40573C1CD0A20A0" modified="2011-06-28" version="1">MMESIYPQRGGIHEQVYLLKRELERRGIKTEIIAYSKRREGQKITLAKIAFPFFHKKNCKGRLRRNNSRDCLDIITINNFLKSLFYTLYSPSTFDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B600</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name evidence="1" type="ordered locus">Ahos_1620</name>
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    <organism evidence="1 2">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        <source>
            <dbReference type="EMBL" id="AEE94498.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="88" mass="9779" checksum="037FB0761311E178" modified="2011-06-28" version="1">MEEWGCSQSGSQISTNFQLLSFSSAPFQVLVDNDFGNLIVEFKRSVEKSFEGEAFVTIGVDENGRIAYISIEPLDKDLKEGIKGIKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B609</accession>
    <name>F4B609_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
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        <name evidence="3" type="ordered locus">Ahos_1629</name>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
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    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="STRING" id="933801.Ahos_1629"/>
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        <property type="taxonomic scope" value="Archaea"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="139" mass="15727" checksum="F36A0442D577898B" modified="2011-06-28" version="1">MFKRILVGFDGSKESLRALNLAINLAKLHGASIKALEVIEHMPVILEAYIKEDTLRDRERIIKHTEIIKRISIENGINIDYEVVRGDPAVVLSKYADSEDFDLIILGKRKLKGLKKVFMESVSSKVLDISKKPVLVVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B627</accession>
    <name>F4B627_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6036 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776440.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B627"/>
    <dbReference type="STRING" id="933801.Ahos_1647"/>
    <dbReference type="GeneID" id="10601147"/>
    <dbReference type="KEGG" id="aho:Ahos_1647"/>
    <dbReference type="eggNOG" id="arCOG05472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106322_0_0_2"/>
    <dbReference type="OrthoDB" id="21412at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045792">
        <property type="entry name" value="DUF6036"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19502">
        <property type="entry name" value="DUF6036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6036" evidence="1">
        <location>
            <begin position="25"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="186" mass="21560" checksum="C036A94E034618D0" modified="2011-06-28" version="1">MLGELLSRIGELKGSETDRFLKFLAILNTLIEERHLGRIIIVGGFAAEIYSGRSYRTGDVDIIIEGEAENLVRQVLKKISDFGLRIYLPKIREISEKGIDIVNNVYNKHKPPLKLQVDSYHVYIIPPEEVVLTYLEAWKFRNSTEDRNKAVLVYCAQRDVMDINYINSESEKRGVKDFFEKIKEYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B635</accession>
    <name>F4B635_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytochrome b558/566 subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B635"/>
    <dbReference type="STRING" id="933801.Ahos_1655"/>
    <dbReference type="KEGG" id="aho:Ahos_1655"/>
    <dbReference type="eggNOG" id="arCOG06016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_884600_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017573">
        <property type="entry name" value="Cyt_b558/566_suB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03155">
        <property type="entry name" value="sulfolob_CbsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="NCBIfam" id="TIGR03155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="272" mass="30191" checksum="55CEBE5EB439813B" modified="2011-06-28" version="1">MNYLGVAGLYMEAVFLGLVAVVLSNKVRSLLPLGIALLVSPSLNLIHNYSLSPYWSFIEIVLALLGIASLIEVTIKSNRRSLLFLPTLAMVTLTTYAGIDTIFLHGDLAICYSFIFIASLLGVIIYAIIYNKIISKRAMMSYIAAIPGLFVFLPLYFLVINNRFLEIIMNMVIPSAFGIVLYNPYNLPILLLALSVSIYTILLLAIKGNGYAGLGYFIIITTAFQAITGFHLLLYLLAPFIGFSILNYREIGNERTIMDDLKKLVQRLSLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B644</accession>
    <name>F4B644_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775248.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B644"/>
    <dbReference type="STRING" id="933801.Ahos_0444"/>
    <dbReference type="GeneID" id="10599884"/>
    <dbReference type="KEGG" id="aho:Ahos_0444"/>
    <dbReference type="eggNOG" id="arCOG00284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453183_0_0_2"/>
    <dbReference type="OrthoDB" id="10575at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="3">
        <location>
            <begin position="164"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="2">
        <location>
            <begin position="490"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="68462" checksum="C259B5AADF97EC9D" modified="2011-06-28" version="1">MEERNLDEEGIIKELENKLNNAEEDAKRNGKLIGRVTRYRTVRLEEEELIGVDISFEDYMRSNVSRGQYLAIRTILRPVIIVGQVVSISRSDVLAEMGIREVTSRKDPASIITDTFLGIYPISEFDEEKNIVRPAVTPIDPQSPVFIPNPSLLEKILRIPEEGITIGKIFSGGEEIEAKVRLDEEALVHHTLVLGTTGSGKTTLLKSILYSKDLDKQTIVFDRQGDFVNFLISKREKFTVLMPVTSKMGNSAKDFLDTFASTYGCKVLEYESGALICNDTEVDVIPYSINFFDNIKNFHKLTPYFTPKASMYWESLIDKTLELLKITLHEIFNVYINDNILDEILRDKVTPASLAEEGNRISISPTLLDELNLRPKSQNISKSFVSFSQGNLTISLNKAFQEAMKALDIATSTKEAIIRTLKAYDGYGIFTVKGTVDFDPEKAFKLNDKIIVDLSFVMNYSASIEAVATIAYKILEELFNWKTELYSKRSLENKLTLIIMDEAHEYFPQGNSEAVAKDIIEELINKIMRLGRVRRIGVILATHMPDDLNPLVLQLTNTKIVMRNDINVIKKMGMENYEDFLLHATPGLAIINSLNFAGITIKTLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B657</accession>
    <name>F4B657_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphate:H+ symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0457</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B657"/>
    <dbReference type="STRING" id="933801.Ahos_0457"/>
    <dbReference type="GeneID" id="10599897"/>
    <dbReference type="KEGG" id="aho:Ahos_0457"/>
    <dbReference type="eggNOG" id="arCOG02689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="22"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="458" mass="49136" checksum="4FFB3465B20D9D61" modified="2011-06-28" version="1">MKLSDVFKPMDDKKFDMFHIKSLLTTGMGVFTDGYDLSSIGIVIGAVLASFGLHSKTPEYNYWEAWVSSSALIGAAIGAIIFGILSNKGRKTFYGIDVALLSIGAVLQAFVSSPLELVLVRGLLGLGVGADYVLSPMIMAEHANAKDRGKIIALGFGLMWGFGATVAAGIYLVLTSLGYSLDTIWRVVLAAGAIPAISVIYLRRKIPETPRYLARIKGDMKAFENVVKQVAGKEVRVNENLKDINSFSEYFKKQWKIFLAAASLWFLFDLSAYTGILFGPTAIAKSVGINNPAVFSFVTEFGFTVPGGLLALATIDRVGRRPMQIIGFVGMAGSLMSFSFFRPLLPAFLALIVYGMYDFFMQAGPGSISAAGMLGVELAPTKVRGTVQSITVASGRIGATTAGLIVPTIILPSVGVDGLTLILSLLTFLAAAITYFFIPETKGKPLEESSMENQYVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B676</accession>
    <name>F4B676_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">4-hydroxybenzoate polyprenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775280.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B676"/>
    <dbReference type="STRING" id="933801.Ahos_0476"/>
    <dbReference type="GeneID" id="10601863"/>
    <dbReference type="KEGG" id="aho:Ahos_0476"/>
    <dbReference type="eggNOG" id="arCOG00477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_5_1_2"/>
    <dbReference type="OrthoDB" id="56630at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000008">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006371">
        <property type="entry name" value="Polyprenyltransferase_UbiA-li"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009609">
        <property type="entry name" value="PRK13106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01475">
        <property type="entry name" value="ubiA_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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            <end position="196"/>
        </location>
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        <location>
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            <end position="266"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="302" mass="33563" checksum="D03AD5730AAD733D" modified="2011-06-28" version="1">MNWDPGAASVNKGKFYTLMRFLRIEQTLFSLPMAYLGAFVAIRGIPPIQTLFLIFSALFFLRIAGMTNDNLADREIDAKNPRTRTRPLVTGAIKVWEAKALIIIGLAGFFISAYFVNIWAFVLSPLVALIVMTYPYMKRYTAFANYHLASIQGLAVFSGAVASAGLYYHSLYCVLKAVPWPFVIATIFWALGFDLYNHIPDAEFDKQMGLHSFAVLLDGFALKFAGLNQLASVILDFLADFMYNLGPIAYASTIAHGLIMAYAYFLANKGNFGSAFYYNIYSSIVLGLGIDIDVLLGIPKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B681</accession>
    <name>F4B681_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase A24B, FlaK domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0481</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B681"/>
    <dbReference type="STRING" id="933801.Ahos_0481"/>
    <dbReference type="KEGG" id="aho:Ahos_0481"/>
    <dbReference type="eggNOG" id="arCOG02298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102058_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052218">
        <property type="entry name" value="Preflagellin_Peptidase"/>
    </dbReference>
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        <property type="entry name" value="Preflagellin_peptidase_C"/>
    </dbReference>
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        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arc_PepC_II"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_A24"/>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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            <end position="23"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        <location>
            <begin position="10"/>
            <end position="121"/>
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    <feature type="domain" description="Preflagellin peptidase C-terminal" evidence="8">
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            <end position="232"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="236" mass="27588" checksum="7FDE5B686ED9CB57" modified="2011-06-28" version="1">MLIYFLQIILTSIMLIHVSILDLKYREVDFKIWLIYLPLVVFFIFYVHRIDLFLYSYSLGVSVFVLYMFYKLSLMGGADLMAILIIGLSNPSVYPLFFPTFSKLGMEPLIVILYSSISVFLVSLYNLVKYFKYTQGMPFSKRIILALSAKRIKVKDFLNSSFLFPLTQVDDNGNVTLRSTFSIEEDDKEWKQKFRKLVEERRISEDSYIWVAWGVPVLPFMLIGYLLSLVLGFPFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B692</accession>
    <name>F4B692_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nudix hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="GeneID" id="10599932"/>
    <dbReference type="KEGG" id="aho:Ahos_0492"/>
    <dbReference type="eggNOG" id="arCOG01075">
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    <dbReference type="HOGENOM" id="CLU_037162_20_2_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
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    <dbReference type="Gene3D" id="3.90.79.10">
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    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
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    <dbReference type="PANTHER" id="PTHR43736">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="Nudix hydrolase" evidence="2">
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            <begin position="3"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="141" mass="15809" checksum="2EAC562AB96F48EC" modified="2011-06-28" version="1">MERPLVAVGGVIFSGKRVLLVQRSKPPNKGSWAIPGGKVEFGETLKDALIREMKEELNVNVEPKELLGVIEIIKEGFHYVILDFICEIKSGEIKAGSDALDAKFFSLEEMSKIPISPTTIEMIRRYFEGEKIPLFITEISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A4</accession>
    <name>F4B6A4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_0504</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775308.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6A4"/>
    <dbReference type="STRING" id="933801.Ahos_0504"/>
    <dbReference type="GeneID" id="10599945"/>
    <dbReference type="KEGG" id="aho:Ahos_0504"/>
    <dbReference type="eggNOG" id="arCOG01956">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_541436_0_0_2"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="454"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="509" mass="56625" checksum="6D45877E81C986C7" modified="2011-06-28" version="1">MSLLIISLLYLGIMLILAKLMEEAFSRLRLVPFVGSIFLGIILGQGVLDFIKINEIISFITSLGIVFLLFLAGAEEIGLNEINTDKSVLISSIIQLILPFTLLFLLLYFLKIPNAILLSIPLGMTSVGPLTRLLLDLGISKEKIGVMLFYEGTIVEITSVIIFAIISNFAKNFAITILEIIGIILGIFLLSPYIAKALEKIEIYINAREVEFASIISLILIISFISETFSFNSAIAALFLGFLLRDYFKDRKDLLDKFHGFTYGFFEPLFFVSIGLYFTKLNFSILSIGIIITAVIVISKFLAGFISATLVKVPRMINALGTSTKGGVDASLLISALSLGLINRIEYSFSALAISISALLVPLIFKATSGYKVKTRQRNKSLIENKISNILPSLKYVTASCEENLRSVIQKINELGVNAIIVCNERKVLGYISTEELLEIEPSKYEELRACDVELHEIITLYDEDKIKKLLDTFREKEVPVIGILNKDGELVGTVYERDLLRLLLTLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C6</accession>
    <name>F4B6C6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776461.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C6"/>
    <dbReference type="GeneID" id="10601168"/>
    <dbReference type="KEGG" id="aho:Ahos_1668"/>
    <dbReference type="eggNOG" id="arCOG06025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154392_0_0_2"/>
    <dbReference type="OrthoDB" id="44073at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="28240" checksum="58E57C002DBE7E54" modified="2011-06-28" version="1">MKMRVRNHALYFIGILSYVVSLIPFSGVNITRALLLIPVIAYTLPILEHLQPKIMTLKIDYKDILLILLAAIPYIFIEINSVIMYIIVPIVLLIVTLTFYFNKNTLWGNVLGTTFVVSLSLVWALFEGNQFLIPDIYWILYIFAGALYVEYKIPYRRLDKSIVQASWILSLVILTLISLNTPLLLVSLIEPSTRFLRPGEKLKSPKEITLLGRKGSKRDVIFVVLLILLSLLSLRLHLYFSLGSPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D1</accession>
    <name>F4B6D1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1674</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776466.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D1"/>
    <dbReference type="GeneID" id="10601174"/>
    <dbReference type="KEGG" id="aho:Ahos_1674"/>
    <dbReference type="eggNOG" id="arCOG00726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="137" mass="15696" checksum="1655E7D668E55410" modified="2011-06-28" version="1">MILDSSAISSFFFRDSFTDKVIKIIESSDEDFLTLDLAFSEVSNVAWKRIVLFNDEKDVVIEQLRNALDFIEKLCEIVSVKDIVMDTINLAVQEKLPFYDSAFLYLAIKEGTKLLTTDIKLFNKLNDKLKSYVIIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E2</accession>
    <name>F4B6E2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin/lipoate A/B protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776477.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E2"/>
    <dbReference type="STRING" id="933801.Ahos_1685"/>
    <dbReference type="GeneID" id="10601185"/>
    <dbReference type="KEGG" id="aho:Ahos_1685"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_1_0_2"/>
    <dbReference type="OrthoDB" id="43646at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="30"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="364" mass="41690" checksum="CEA8EE40A022E20A" modified="2011-06-28" version="1">MSWRFAALPPQDGYHMVTSFVSVADYVSRGGKDTLMVFYAKEPFVNVGVNQEVWLEVNLDFTKQHNIPVVRRDLGGGTVVITPGEHDFFIVIKQEEAPSDPQSLYKKYLTPIVNVIRSYGLDAQLRDQDIVVNGKKISGNGAMTYEKAVVIAGNILLSFDADFVSKCIKVPSEKFRDKIAKDMSEWLTSMEKELGYVPDRNELNKKIKEEFEKELGIKFEDSSLTPEEIELWDKLAEEKKREEWIYYKDNRHPDLRTDRCVKISSAVAVCHVDYKARKLIRITVKIVNKKIDEVSISGDFFLMSPKGFLDRLEDSLQGIEVSKINEKIDEVFSSEKPVIFGFTADDLKKAFEEILNKPEIREII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F3</accession>
    <name>F4B6F3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heterodisulfide reductase, subunit C (HdrC-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1696</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776488.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F3"/>
    <dbReference type="STRING" id="933801.Ahos_1696"/>
    <dbReference type="GeneID" id="10601196"/>
    <dbReference type="KEGG" id="aho:Ahos_1696"/>
    <dbReference type="eggNOG" id="arCOG00965">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086560_0_0_2"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="277" mass="32302" checksum="1D4FB3F46C550D7E" modified="2011-06-28" version="1">MATKVLQLPDDPRFLDMSYEEQGALPEEQRNLDRLPPDQRQLAEEFWKAVKSDFRFNEYLRGCLNCGVCTSGCPAAKFYDFGPREMIQYMMRDEADKIWEFVNKKVWACVQCYTCSMRCPFNNEIAGLIMLLREYAVQFALPSAKEILAPYRRVLYTVLTLGNQVTPDMIQPEAFPDWGPQAVEESKNMDVYRKAVPVDLLQRTDVGWHPSLQTAVEMMTIMFESGVMDSIKKVDPDLYDMIADIYDERKQQLEEIKEKWEKGELKEEDLPDSWLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S5</accession>
    <name>F4B6S5_ACIHW</name>
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        <submittedName>
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    <organism evidence="1 2">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776533.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S5"/>
    <dbReference type="STRING" id="933801.Ahos_1741"/>
    <dbReference type="GeneID" id="10601241"/>
    <dbReference type="KEGG" id="aho:Ahos_1741"/>
    <dbReference type="eggNOG" id="arCOG07299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848874_0_0_2"/>
    <dbReference type="OrthoDB" id="40423at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022297">
        <property type="entry name" value="CRISPR-assoc_prot_CsaX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03876">
        <property type="entry name" value="cas_csaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="327" mass="36869" checksum="263AB204480A75EF" modified="2011-06-28" version="1">MRIPLYNIFGDSYVKQVAIEAGKYEILRNAIEIDDNSLKEVLKIAGDIAKSREEKIKNKIVNILPLSGNDKRAWEKILKCYNFGNIATLSEVLYKFSPEAEKCNVDTAPSFVKPEFYEYARIPGIPGGSKSKMSVDGSYLVVAAAGWVLTRLGKVKVNEGNWLGVQLFTTTKSNLYKILENIKYIPGIKPETAFAIWIADKIINVNLNVYTLKVYLISDAVGQSPTTIEEGFVLDLNKLLMNKEMINDDIVYIASDALNIDSNTRNYSAKIINLVYEVISGSKRIEDLLYFANRDLIMISESNDERIPLLKRISRYANYLSNVTTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W2</accession>
    <name>F4B6W2_ACIHW</name>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1779</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776570.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6W2"/>
    <dbReference type="STRING" id="933801.Ahos_1779"/>
    <dbReference type="GeneID" id="10601279"/>
    <dbReference type="KEGG" id="aho:Ahos_1779"/>
    <dbReference type="eggNOG" id="arCOG08498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009980_0_0_2"/>
    <dbReference type="OrthoDB" id="43758at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="13868" checksum="D5716C145798BCB6" modified="2011-06-28" version="1">MRLGIILISVGILILVLGEISFPLHTSLTVTHHFTSPPWFTSGKIYVNNGTFTVYSDYLVENLTNGQSICLNNFTLIGKGNATVTFSGFKIFYTRNYITFSEIVIILGIVLEILRIIRLRLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X7</accession>
    <name>F4B6X7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X7"/>
    <dbReference type="KEGG" id="aho:Ahos_1794"/>
    <dbReference type="eggNOG" id="arCOG03673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617674_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="443" mass="50089" checksum="3E3C816E383801E6" modified="2011-06-28" version="1">MKIFQETITLSKNKYFMSDILYVKRLALLLLPLLLLLYPPLHAVLIGPTDYGYEFFQYQLCGITARITFYCLNYTGKIPSVQQNFCIGLNASQIFVQNVMWYIGKCFNPSWETSLYYNGSYHIFMMTPDLTGNTYNLTTLIYNGTGYVKIVFLISNYTNTFKHCVTLKGIYRGIVGSLEGTVIAGYGCGETVTLEKGTKLEISSLYLYKGKWYVPAIILGNAPDTGEAAYCGKVCLLPCNTVLVTYNDSHGEYCIDYLALPSVIIFNNTVKVFPYNSLWEIEYPNGSTRYFVNCTTFVEGSRVCPVDVVGNYTFLLNKTYLVKSCIPIYGEKEFYIPEAEYLFYVENGSYHKIFVNGSETILFHNSSSSYNTTTSVRFTNTNLQTSTTTSNNLTSSEQSIISTRLYKNPHSPTREFLIISFIALIVIVALLLLVLRGNKGLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X8</accession>
    <name>F4B6X8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clan AA aspartic protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X8"/>
    <dbReference type="KEGG" id="aho:Ahos_1795"/>
    <dbReference type="eggNOG" id="arCOG04029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161799_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022274">
        <property type="entry name" value="Peptidase_asp_AF0612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03698">
        <property type="entry name" value="clan_AA_DTGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11731" checksum="936AC40347DA2434" modified="2011-06-28" version="1">MTDLKNSVEIDALVDTGFSGWLLLNYEIYKKLNSLELPITRKYRGIIGNVEVYVARANIIIGNLNVDAFIESSPYVEMNLIGRELLKRLNICFYRVNKICIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z4</accession>
    <name>F4B6Z4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Exodeoxyribonuclease III Xth</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1812</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776602.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z4"/>
    <dbReference type="STRING" id="933801.Ahos_1812"/>
    <dbReference type="GeneID" id="10601312"/>
    <dbReference type="KEGG" id="aho:Ahos_1812"/>
    <dbReference type="eggNOG" id="arCOG02207">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_1_3_2"/>
    <dbReference type="OrthoDB" id="146626at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="7">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="245" mass="28776" checksum="86EF59D38B0519C8" modified="2011-06-28" version="1">MKIVTWNVNGLKAITRKGSLDEVLKYDVVMLQEIRTSDLPLDLLFSGLAIESFSAKKKGYSGVMTLTRYKPINVIKGLNVEEFDEEGRVLTLEFEKLFLINSYFPRAGDELKRLDFKIKFDKTVEEFMMKLRERKPVIICGDFNAVRDRKDSSFWDEREPALTPQEREWLNHVINDLGFIDAYKLVNPNKNEFTWRSYRFKWKAMRIDYCLVSSELKNEIKNCEVLKIEGSDHYPLLLELNIESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B702</accession>
    <name>F4B702_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved archaeal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B702"/>
    <dbReference type="STRING" id="933801.Ahos_0597"/>
    <dbReference type="KEGG" id="aho:Ahos_0597"/>
    <dbReference type="eggNOG" id="arCOG04123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142667_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014450">
        <property type="entry name" value="UCP008210"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09921">
        <property type="entry name" value="DUF2153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008210">
        <property type="entry name" value="UCP008210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="126" mass="15054" checksum="E11A06B84F11D3C8" modified="2011-06-28" version="1">MQKSLLATIKDMEKNENDKDMDRLDLILASRVAFQHMMRTIKAFDQWLQDPMIIKHMPKEMLEDVKRTSWSILEQLLELDIRHTSEARDLINKLGKEGKLDPLIWSRPLFDEQQNQNNRRITFTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F4B705</accession>
    <name>F4B705_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CTP synthase</fullName>
            <ecNumber evidence="11">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="11">CTP synthetase</shortName>
            <shortName evidence="11">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyrG</name>
        <name evidence="14" type="ordered locus">Ahos_0600</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UTP + NH4(+) + ATP = CTP + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775404.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B705"/>
    <dbReference type="STRING" id="933801.Ahos_0600"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="GeneID" id="10600047"/>
    <dbReference type="KEGG" id="aho:Ahos_0600"/>
    <dbReference type="eggNOG" id="arCOG00063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_2"/>
    <dbReference type="OrthoDB" id="52769at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000009">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000002">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="12">
        <location>
            <begin position="302"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="11">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="11">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="507"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="509"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="383"/>
            <end position="386"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="531" mass="59096" checksum="F25D9B6D4FF10122" modified="2011-06-28" version="1">MTKFVIVTGGVLSSVGKGTVAASIGLLLKARGYKTSVVKVDPYINVDAGTMNPYMHGEVFVTDDGAETDLDLGHYERFLDIDMSKHNNITAGKVYFEVIKKEREGKYLGQTVQIIPHVTDEIKSMIRKVAELDKADIVIVEIGGTVGDIESLPFLEAVRQMKLEEEEHDIVFIHVALVEYLNVTGELKTKPLQHSVQELRRIGIQPDIIIARSIVPIDEETKKKIALFTNVKPEYIFSNYDVSTAYEVPLILEKQSLPSKLLSKLALKDTPPKLDDWVNFVNAIKNGNKVVRIGLVGKYTKLKDSYLSIKEAIYHASAMLHLKPQIVWIESTDLEKDNAVEEKLKGLDGIVVLPGFGARGVDGKLKAIKYARENNVPYLGICYGLQLAVVEFARNVLGLKGAHTTEVDPNTPYPVVTLLDEQKRVTQVGGTMRLGAQKIILKEGTIAYNLYGKSEVYERHRHRYEVNPEYVDKLQSAGMIISGVSENGLVEMIELKDHKFFVATQAHPEFKSRPLRPSPLFVGFLKAAAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B762</accession>
    <name>F4B762_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps9</name>
        <name evidence="6" type="ordered locus">Ahos_0658</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775461.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B762"/>
    <dbReference type="STRING" id="933801.Ahos_0658"/>
    <dbReference type="GeneID" id="10600109"/>
    <dbReference type="KEGG" id="aho:Ahos_0658"/>
    <dbReference type="eggNOG" id="arCOG04243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="OrthoDB" id="52677at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_uS9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_uS9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="139" mass="15916" checksum="BBA98F5A21DDE00B" modified="2011-06-28" version="1">MSEQQANKIIITSARRKMARAQCQLTEGKGRVFVNGVPIELIPIEMIRLKIMEPLLLAGESLASKIDARIYTFGGGIMGQADAARMALARALVKFSDSKELEELYKQYDRTMLAGDPRETESEKWMRYSARRWRQKAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B779</accession>
    <name>F4B779_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1824</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B779"/>
    <dbReference type="STRING" id="933801.Ahos_1824"/>
    <dbReference type="KEGG" id="aho:Ahos_1824"/>
    <dbReference type="HOGENOM" id="CLU_2820559_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="66" mass="7646" checksum="BE661AC27900C4D9" modified="2011-06-28" version="1">MTECRDLAINHRWVMGLQRHGAHISAPTHIGVRDHYLSTKIRYKQTKKISINMKKDSPLLQTACVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B781</accession>
    <name>F4B781_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776616.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B781"/>
    <dbReference type="STRING" id="933801.Ahos_1826"/>
    <dbReference type="GeneID" id="10601326"/>
    <dbReference type="KEGG" id="aho:Ahos_1826"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_1_2"/>
    <dbReference type="OrthoDB" id="359241at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="121" mass="13950" checksum="418F3DA03075FFF2" modified="2011-06-28" version="1">MKRAEDWIKQAERDLEEAEYAESGGYYELACSQQCAEKAVKGLLQFRGVEKRGHSISHLLENPPEDVLQCAIFLDKQYTPSRYPDVYDEGSPYEYYTQKDAEECINCAIKILNWVKGETSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B799</accession>
    <name>F4B799_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pyrimidine dimer DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776634.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B799"/>
    <dbReference type="STRING" id="933801.Ahos_1846"/>
    <dbReference type="GeneID" id="10601345"/>
    <dbReference type="KEGG" id="aho:Ahos_1846"/>
    <dbReference type="eggNOG" id="arCOG05276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120482_0_0_2"/>
    <dbReference type="OrthoDB" id="70703at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004260">
        <property type="entry name" value="Pyr-dimer_DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03013">
        <property type="entry name" value="Pyr_excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="151" mass="17984" checksum="90CC10C19FEC058D" modified="2011-06-28" version="1">MRLWSIHPKYLDAHGLLGLWREGLLAQKVLLGETRGYKNHPQLIRFKRTSDPVLYIGTYLYYVYLEGVRRGYSFNKEKIVKYDLTLRMPVTEGQINYEFRHLLEKLKIRNPKMYAELLQVKITEVNPIFFVIKGDVEEWEKVAFFNTQNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A9</accession>
    <name>F4B7A9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776644.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A9"/>
    <dbReference type="STRING" id="933801.Ahos_1856"/>
    <dbReference type="GeneID" id="10601355"/>
    <dbReference type="KEGG" id="aho:Ahos_1856"/>
    <dbReference type="eggNOG" id="arCOG06052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616236_0_0_2"/>
    <dbReference type="OrthoDB" id="41180at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="50302" checksum="B9C1BD70B23BEBEC" modified="2011-06-28" version="1">MASSSAVYEFCHGQWIKILQMNNIKQVEGGKDGIYVNNGTLYYFYNLTRLLPLNITNPFKLFYDNYTGELYVTSNSHLYIINCSRVIASYYIPLSFCYVTFNCKEAIVDSQCGYFIFFYNGSEHLTISCQDAFAGMLFAEGKFITGSFDPRGLFIYNNLSVLYLNKIWDIYLRQLPEYNITLVCTKVTPDCLIYFKGMLYISSLQGIQVINLENYQSVYYNPCPAYSMIIYNCSVLVATKSGIITLNISPLPIYTLTIKKLGGLYGKVLINGTEYTFYNCLKLRLEEGIYNLTFLNCSIYKPCINNEVINLNCNKTLTIKYVGIPEELSVILEGLNNGSRYSITINNQTYIEDSNMVKFTLPAGQYCIKIDINGVTKSFIINLIQDEEIVINASSFYLTTNNSTITATSHIGSNSNNSLSLACYIAIALVVVALIFIIIVIRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C2</accession>
    <name>F4B7C2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776657.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C2"/>
    <dbReference type="GeneID" id="10601368"/>
    <dbReference type="KEGG" id="aho:Ahos_1869"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_622033_0_0_2"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="440" mass="50803" checksum="15D448CBE1253B85" modified="2011-06-28" version="1">MDIFTTDARPFPEYLYGREDEIKRLISLVNTEDFHIALIGGMRRTGKTSLLLSLLKIFTHSKYKEEYNINDAYFPLYIEMDAVKSLEDFNSKLYNRILSNHTWRLIQNTKVEKKVKNVINCFKEKYETFKGYNLSIPSVIGIGEQKGYQDPYASSISRILESLSSCLYDEKVKFGLVVIDEFQKILDWKDEEQQTFIEILKEIYDVYHNLKVFITGSVISSTEKLFSTEYDKPMHGRRIDKIYLMPISVEASKKMLEDGFREHNINDELAVTSGVTIGMGIPGLLTYYGKFLVNYYSKENPEKAIEDASRDAYIQLGKEVEEEITHLKKIKDDSRYLEASKIIVREGGKIIPSKLAEFLKISVNSAEIILNKLTEIGFINKKNNEYEVVDNTLITLLYNEVSFKWDLCPICNKGKSLIQPFVNTVLYSCGHVVAVRKIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D3</accession>
    <name>F4B7D3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfocyanin-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1880</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776668.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7D3"/>
    <dbReference type="STRING" id="933801.Ahos_1880"/>
    <dbReference type="GeneID" id="10601379"/>
    <dbReference type="KEGG" id="aho:Ahos_1880"/>
    <dbReference type="eggNOG" id="arCOG03700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105306_0_0_2"/>
    <dbReference type="OrthoDB" id="56344at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049544">
        <property type="entry name" value="SoxE-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06525">
        <property type="entry name" value="SoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfocyanin-like C-terminal" evidence="4">
        <location>
            <begin position="70"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="226" mass="24247" checksum="436A44B15CC02EC3" modified="2011-06-28" version="1">MNKAVSTSVVIVVAIALILVAVAGYYLATRSPIPEGTTTLPPSTVTTMTQTTTPVTTTTPVTTTTSALPPGAMPLPYNPSTKTVYLYISTWDNATLYNFNGTSDGELHLFIPAGWKVCVIYTNFEAIDHNFIIVKNTTAVPSCSIIPSSNIILYVGVSSSNYFDSGIASGASATGSIVLSPGYYWFACGYEDHAESGMWGVIECSSSITQPYYEVTNEEIEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G3</accession>
    <name>F4B7G3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcription initiation factor IIB</fullName>
            <shortName evidence="7">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tfb</name>
        <name evidence="9" type="ordered locus">Ahos_0681</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775484.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G3"/>
    <dbReference type="STRING" id="933801.Ahos_0681"/>
    <dbReference type="GeneID" id="10600133"/>
    <dbReference type="KEGG" id="aho:Ahos_0681"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="120"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="120"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="214"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="214"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="304" mass="34288" checksum="15B0371003FDE95C" modified="2011-06-28" version="1">MSDKPQDNICPPDKIVFDADRGEYICTETGEVIEERIIDQGPEWRAFTPEEKEKRSRVGGPLNQTIHDRGLSTLIDWKDKDAIGRNLDPKRRLEVLRWRKWQIRARIQSSIDRNLAQAMNELERIGNLLGLPKSVKDEAALIYRKAVEKGLVRGRSIESVVAASIYASCRRIKIARTLDEIAQYTKANRKEVARCYRLLLRELNVEVPVSDPKDYVTRIGSLLGLSGATMKLAAEILEKAKNAGLTAGKDPAGLAAAAIYIAALMNDERRTQKEIAQVAGVTEVTVRNRYKELIQELKIEIQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G9</accession>
    <name>F4B7G9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0687</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775490.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G9"/>
    <dbReference type="STRING" id="933801.Ahos_0687"/>
    <dbReference type="GeneID" id="10600139"/>
    <dbReference type="KEGG" id="aho:Ahos_0687"/>
    <dbReference type="eggNOG" id="arCOG05942">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1901920_0_0_2"/>
    <dbReference type="OrthoDB" id="42720at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="134" mass="15445" checksum="379570217B5B576F" modified="2011-06-28" version="1">MNDSDTQVNIALTHFETLIEDHSTYLNELENLSVIPQMDMDRVMRIIKRMRKIRKDLELGINTILTYIDSVGNSRIKEEAIGIISYLNIVGFKDEKEILQKLSTQAKEMGYDINVDDDIKQIDNILSKISKISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H1</accession>
    <name>F4B7H1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="4">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">endA</name>
        <name evidence="7" type="ordered locus">Ahos_0689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775492.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H1"/>
    <dbReference type="STRING" id="933801.Ahos_0689"/>
    <dbReference type="GeneID" id="10600141"/>
    <dbReference type="KEGG" id="aho:Ahos_0689"/>
    <dbReference type="eggNOG" id="arCOG01701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114393_0_0_2"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000006">
        <property type="entry name" value="tRNA-splicing endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.20">
        <property type="entry name" value="tRNA intron endonuclease, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01833">
        <property type="entry name" value="EndA_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="tRNA-intron endonuclease N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="5">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="183" mass="20777" checksum="131ABBDF6C30C876" modified="2011-06-28" version="1">MSIKGYLFGDKIVIFDINDSKKLYSTGFYGKPLGINKPKKAEDIKYPLELSLIEGLYLLKKGEIEVIYNGNKLNENDLYEIGRKNIARFDSLFTVYSDLRNKGFVVRSGIKFGADFAIYTLGPGIEHAPYVVIVLDANSTLLANELMSFGRVSHSTRKKLILAIVNMLPPKNIRYVMFKWVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J0</accession>
    <name>F4B7J0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AAA family ATPase, CDC48 subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0708</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775511.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J0"/>
    <dbReference type="STRING" id="933801.Ahos_0708"/>
    <dbReference type="GeneID" id="10600161"/>
    <dbReference type="KEGG" id="aho:Ahos_0708"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19529">
        <property type="entry name" value="RecA-like_VCP_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF199">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="7">
        <location>
            <begin position="6"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="6">
        <location>
            <begin position="104"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="210"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="485"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="750" mass="83588" checksum="DF0F5875D4701DC2" modified="2011-06-28" version="1">MSAPIKLKVSEARQRDVGRKIGRLSENLMSKLKIDAGDYLEIIGPSGSSLVQAMPAYDVSDDEIRIDGYIRKAIGASIGDEVEIRKATVNKATKIVLAPTQPIRFDQSFVDYVKDQLMYKPLVKGETIPIPIYTGVIELVVVNTQPSNYVFVSSETQLDIKEEPVKGETTYAKVTWEDIGDLEDVKERIREIVELPMKHPELFQRLGIEPPKGILLYGPPGVGKTLLARALANEIGAYFISINGPEIMSKFYGESEERLRQIFDEANKNAPSIIFIDEIDAIAPKREEVTGEVEKRVVAQLLTLMDGIKGRGKIIVIGATNRPDAIDPALRRPGRFDREIEIRPPDTKARKEILQVHTRSMPLSDDVNLDDIAEMTNGYTGADLAALAKEAAMVALRRFLATTKVNLDQGQIPAELLKELKVTMNDFLEAMKSIQPTLLREVYVEVPKVRWSDIGGLEDVKQQLREAIEWQIKFPDVFTKSGIRAPKGVLLFGPPGTGKTMLAKAVATESGANFIAVRGPEVLSKWVGESEKAIREIFRRARQTAPTVIFFDEIDSIAPMRGFAHDSGVTERIVNQLLAEMDGITPLNKVVVIAATNRPDILDPALLRPGRFDRLIYVPPPDKIARLEILKVHTRNVPLAEDVNLETIAEKTEGYTGADLEAVVREATMLMLREVSAVCEQKSREACTKDGKFVEECYNKEMRNCMNNFSGKVSMKHFEEALKIVSPSITKADIERYERLAKELKRSAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P0</accession>
    <name>F4B7P0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P0"/>
    <dbReference type="KEGG" id="aho:Ahos_1914"/>
    <dbReference type="eggNOG" id="arCOG03769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147147_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="14333" checksum="7D81DF2EDD975C63" modified="2011-06-28" version="1">MHIPDIVDVIIGFLISWIIISIPVWLASKLFSSESSFPRAMAASLISIIVFAIIVAIFAFIALVIHNPFVMPIGFIVGLIGILGVYKAIFDVGWLGALGIAILAFIISIVIDLILAAVGLEAFTITDFLHIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q7</accession>
    <name>F4B7Q7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1931</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776719.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Q7"/>
    <dbReference type="STRING" id="933801.Ahos_1931"/>
    <dbReference type="GeneID" id="10601430"/>
    <dbReference type="KEGG" id="aho:Ahos_1931"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="6"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="421" mass="46359" checksum="67FD3A9FAC77D6DD" modified="2011-06-28" version="1">MQKIRPFFVSSAGFFLDGYDLSVISFALYFIAQEMKLSSSQEGLVSSASLMGMTIGALLFGFLGDKVGRKKLMGIDLIFFMTFGITSALSQNFTQLFISRLLIGIGIGGDYPISSTIMSEFSPAVNRGKYLVASISLYWVGTLVASIVNLTFLSFYEFWRYTFLFGALISLPIILLRIKLDESPRWLASKGMIVTEKFNPQVENKGVNGIKELFENPLLPYVLAVSMVWFLFDIASYGIGLYYPLVLREFAFPSNVEVLYSTMAIAGGALLGYLVAVSAIDSLGRRKVLLIGLGGMAFLLIIGGITKIEGFLLVPYFMTFVALEQWAGAVTLFYPTEIFPTSVRSTAQGFATSVSRIGAILGVYFFPSMVRSVGFSSSLIIFGITSTLALVISFLTYRETRKKELEETSLGKIIISNNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S4</accession>
    <name>F4B7S4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1948"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HEPN_dom"/>
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        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
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    </dbReference>
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        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="55" mass="6568" checksum="C1E68A7101ABB6DA" modified="2011-06-28" version="1">MDFLKRNALDFLKYAELLFNNQNYNLAMFSLEQSLQLALKYYLSELTARFLVPMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S7</accession>
    <name>F4B7S7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pyruvate ferredoxin/flavodoxin oxidoreductase, delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1951</name>
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    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776739.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S7"/>
    <dbReference type="STRING" id="933801.Ahos_1951"/>
    <dbReference type="GeneID" id="10601450"/>
    <dbReference type="KEGG" id="aho:Ahos_1951"/>
    <dbReference type="eggNOG" id="arCOG01605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
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        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
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        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="86" mass="9744" checksum="C3456CF47F417243" modified="2011-06-28" version="1">MIDYPVPLGRPKIGSSGLTGHWRLMRPEIDYSKCTKCRLCVIYCPENTIDLLEGFDVRIDYDYCKGCGVCAQICPQKAIQMVPEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V3</accession>
    <name>F4B7V3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusA</name>
        <name evidence="9" type="ordered locus">Ahos_0744</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V3"/>
    <dbReference type="STRING" id="933801.Ahos_0744"/>
    <dbReference type="KEGG" id="aho:Ahos_0744"/>
    <dbReference type="eggNOG" id="arCOG01760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131906_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22531">
        <property type="entry name" value="KH-II_NusA_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058582">
        <property type="entry name" value="KH_NusA_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="TF_NusA_bacterial"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648:SF0">
        <property type="entry name" value="TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26594">
        <property type="entry name" value="KH_NusA_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="20"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="130" mass="14523" checksum="F67B39CD14434B13" modified="2011-06-28" version="1">MSLFQEVTKVTAKDCVVDEENNRIIFLVDPHFMGLAIGKNGMNVKKLKKVLGKDIEIVAYSDNLEDMVKNLMSPARVRSVKVIDNDSKKTVYITVDPQDKGIAIGKSGKNVVRAKLILKRYMDIDSVVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V5</accession>
    <name>F4B7V5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo1C</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit A''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo1C</name>
        <name evidence="8" type="synonym">rpoA2</name>
        <name evidence="10" type="ordered locus">Ahos_0746</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775548.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V5"/>
    <dbReference type="STRING" id="933801.Ahos_0746"/>
    <dbReference type="GeneID" id="10600203"/>
    <dbReference type="KEGG" id="aho:Ahos_0746"/>
    <dbReference type="eggNOG" id="arCOG04256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037097_1_0_2"/>
    <dbReference type="OrthoDB" id="372142at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06528">
        <property type="entry name" value="RNAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00411">
        <property type="entry name" value="RNApol_arch_Rpo1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012757">
        <property type="entry name" value="RPO1C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02389">
        <property type="entry name" value="RNA_pol_rpoA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 8 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase Rpb1" evidence="9">
        <location>
            <begin position="19"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="392" mass="43509" checksum="BD105585207388E6" modified="2011-06-28" version="1">MINEEDKKYLEEKLSSLSGKIPGSVIQKLRDAIISYPANITKEEIDKIINLTLKDYDVSLINPGEAAGVVAAQSIGEPGTQMTLRTFHFAGVRELNVTLGLPRLIEIVDARKVPSTPMMTIYLTDEYKTDREKAISIARKIEYTKIENVVDSTSIDMASMALIIHLDEKMLKDKGIEPDEVEKVIRKLKLGDFTIDRPDELTITVTFQNMDSITGLFKAREKILNTKIKGIKGIKRAIVQKRGDEYVIITDGSNLEGVLGIKGVDVSRIETNNLHEVENVLGIEAARELITREIKKVLDDQGLDVDIRHVELVADIMTRTGEVRQIGRHGVTGEKTSVFAKAAFEVTVKHLLDAAARGDVEEFRGVVENIIIGQPIKLGTGMVELLMKPGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z5</accession>
    <name>F4B7Z5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="10">GGGP synthase</shortName>
            <shortName evidence="10">GGGPS</shortName>
            <ecNumber evidence="10">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0786</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775588.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z5"/>
    <dbReference type="STRING" id="933801.Ahos_0786"/>
    <dbReference type="GeneID" id="10600246"/>
    <dbReference type="KEGG" id="aho:Ahos_0786"/>
    <dbReference type="eggNOG" id="arCOG01085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068610_0_0_2"/>
    <dbReference type="OrthoDB" id="7409at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120536">
        <property type="term" value="F:heptaprenylglyceryl phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.390:FF:000001">
        <property type="entry name" value="Heptaprenylglyceryl phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039074">
        <property type="entry name" value="GGGP/HepGP_synthase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003198">
        <property type="entry name" value="PRK04169.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003202">
        <property type="entry name" value="PRK04169.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029:SF2">
        <property type="entry name" value="HEPTAPRENYLGLYCERYL PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="172"/>
            <end position="178"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="248" mass="27092" checksum="D8DEFCDC3375FAC6" modified="2011-06-28" version="1">MKEKISKYIQQLSEEGVLHFSLIDPDKISNLDKLDSLARKLYEAGTSAFLIGGTLGVSKDKLDNVLEILSDIKIPKIIFPSNINLISEKADAILFLSLLNSDDIYYVIGAQVIAAPLIKKLGIEVLPTGYLIIGYGGTAGHIGRARIIPFDNNELALSYALAAEFMGMKFLYLEAGSGSPETVRAEMVSMIRRFTNLTIIVGGGIRSQEAAIKLVEAGAHIIVTGNIIEDNPEKAIKIIESIRNYRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B805</accession>
    <name>F4B805_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thermosome subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Chaperonin subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Thermosome subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0796</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775598.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B805"/>
    <dbReference type="STRING" id="933801.Ahos_0796"/>
    <dbReference type="GeneID" id="10600257"/>
    <dbReference type="KEGG" id="aho:Ahos_0796"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000014">
        <property type="entry name" value="Thermosome subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="554" mass="59539" checksum="C016CB66B15DF15A" modified="2011-06-28" version="1">MSSTATVATTPEGIPVIILKEGSSRTYGKEAVRLNIAAVKAVEEALKSTYGPRGMDKMLVDSLGDITITNDGATILDKMDLQHPAAKLLVQIAKGQDEETADGTKTAVILAGELVKKAEDLLYKDIHPTIIISGYKKAEEVALKTIQEIAQPVSINDTDLLKKVAMTSLSSKSVAGAREYLADIVVKAVTQVSELRGDKWYVDLDNIQVVKKAGGGINDTQLVYGIIVDKEVVHPGMPKRIENAKIALLDASLEAEKPELDAEIRINDPSQMQKFLEEEDNLLKEKVDKIAATGANVVICQKGVDEVAQSYLAKKGILAVRRAKKSDLEKLARATGGRVVSNIDELSPQDLGYAALVEERKVGEDKMVFVEGAKNPKAISILIRGGLERVVDETERALRDALGTVADVIKDGRAVAGGGAIEIEIAKALRKYAPQVGGKEQLAIEAYASALESLVMILIENAGFDPIDLLMKLRSAHENENNKWVGVDLYTGQPVDMWQKGVIDPALVKMNAIKAATEAVTLILRIDDLVAAGKKSTSSGSESKSGESKPSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B806</accession>
    <name>F4B806_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TFIIB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775599.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B806"/>
    <dbReference type="STRING" id="933801.Ahos_0797"/>
    <dbReference type="GeneID" id="10600258"/>
    <dbReference type="KEGG" id="aho:Ahos_0797"/>
    <dbReference type="eggNOG" id="arCOG05931">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393599_0_0_2"/>
    <dbReference type="OrthoDB" id="27163at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="170" mass="20057" checksum="E065B4EB6385C941" modified="2011-06-28" version="1">MQCPYCNSKDLIWDSIHGNIICASCGSVIDTLYDYSQIGTSEEIIDLNKGVSERKETRNFIKLTETISNKFRIFNNKENMKFITEDGELALSLLNMDKKVYKIYEFLEKNGMFSGRKIKIRVALSFYIAGYRCKKMKNILEKLHINEKYFRKITYKLPKELKYMNKELLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B815</accession>
    <name>F4B815_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="10">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pan</name>
        <name evidence="13" type="ordered locus">Ahos_0806</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775608.1">
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    <dbReference type="GeneID" id="10600267"/>
    <dbReference type="KEGG" id="aho:Ahos_0806"/>
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    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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    <dbReference type="InterPro" id="IPR023501">
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    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
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    </dbReference>
    <dbReference type="NCBIfam" id="NF003069">
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    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01242">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
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    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF17862">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
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    </dbReference>
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    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="166"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="5"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="177"/>
            <end position="182"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="316"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="390" mass="43746" checksum="091E131891BD4C26" modified="2011-06-28" version="1">MSGEIDVSRDNNEDYEQIIKLLEEKINALQAETESLRKELNYYKSELEKLLSPPLIEAVVLDILDDGKVVVRSTSGPNLIVTVSSDIDIKSLKIGQSVALNQRGSAIVKILPEREDPFVKSMEILEKPNVRYEDIGGLSQQIQELREVIELPLKNPEIFKELGIQPPKGVLLYGPPGTGKTMLAKAVATESNATFIHVVASEFAQKFVGEGARVVREVFELARKKAPSIVFIDEIDAIGAKRIDLGTSGEREVQRTLMQLLAELDGFQPLDNVKIIGATNRIDILDPALLRPGRFDRIIEIPLPDFNGRKEIFRIYISKMKVSRDVDINILAKLTEGFSGADIKNVCTEAGYLAIRENKRIIEMNNFIEAINKVKSKRKEEFKGREEKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B845</accession>
    <name>F4B845_ACIHW</name>
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        <submittedName>
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="10601496"/>
    <dbReference type="KEGG" id="aho:Ahos_1997"/>
    <dbReference type="eggNOG" id="arCOG02217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175270_0_0_2"/>
    <dbReference type="OrthoDB" id="45710at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="InterPro" id="IPR039709">
        <property type="entry name" value="VapB3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244:SF2">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="70" mass="8413" checksum="FD3869E8C316E822" modified="2011-06-28" version="1">MSQLIKLPKDLKEKMVKYNINWNSIIREFVYEKIEELERQEHARRAVEILKSLNFSYEGAKEVVKNRNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B856</accession>
    <name>F4B856_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B856"/>
    <dbReference type="STRING" id="933801.Ahos_2008"/>
    <dbReference type="KEGG" id="aho:Ahos_2008"/>
    <dbReference type="eggNOG" id="arCOG09929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179760_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="9952" checksum="95452ED9EC193046" modified="2011-06-28" version="1">MLMTLLPSSIGGLMGLWEEQAVSLSFKGLELSDESSLSSSGGVSDYPDCPPYERCSPELMVGTMSPLGGNPRPSRAGRSQLLTALKDSIYRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B867</accession>
    <name>F4B867_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776806.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B867"/>
    <dbReference type="STRING" id="933801.Ahos_2019"/>
    <dbReference type="GeneID" id="10601518"/>
    <dbReference type="KEGG" id="aho:Ahos_2019"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521785_0_0_2"/>
    <dbReference type="OrthoDB" id="34387at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon" evidence="1">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="19834" checksum="223B435B4CE75C1C" modified="2011-06-28" version="1">MNKTFQGVYVDPYQLGMQLETWLAGKGYKTQILRAGNYVVVQAKKEGILRTIFGADRAFTVRLYGGQGFLQVDIGMSNWLKAGEAAEDIVAAMVFMPLAAVEGVEEIYNVKIEHDIMKEIERLVSQTTFQPTFNQPMFQPMTQQPMMPPMQEKICNACGFRNPPHARFCMNCGNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G2</accession>
    <name>F4B8G2_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775677.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G2"/>
    <dbReference type="STRING" id="933801.Ahos_0875"/>
    <dbReference type="GeneID" id="10600346"/>
    <dbReference type="KEGG" id="aho:Ahos_0875"/>
    <dbReference type="eggNOG" id="arCOG08309">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662536_0_0_2"/>
    <dbReference type="OrthoDB" id="35979at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="80" mass="9180" checksum="6B5AC62531458968" modified="2011-06-28" version="1">MKSVYGSTILESAGIFDLQKNEKDAKISYNEAKRLYPDFKVLILDMNKIEDRLKAIDIDPDLADMKDIYVILVEVPEEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G4</accession>
    <name>F4B8G4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)/FAD-dependent oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775679.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G4"/>
    <dbReference type="STRING" id="933801.Ahos_0877"/>
    <dbReference type="GeneID" id="10600348"/>
    <dbReference type="KEGG" id="aho:Ahos_0877"/>
    <dbReference type="eggNOG" id="arCOG05933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978674_0_0_2"/>
    <dbReference type="OrthoDB" id="36755at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="32986" checksum="E35665609FCFC646" modified="2011-06-28" version="1">MNIILGGGISGLLLASLNKEESLILELQHEVGGLFATEEISGTNISIIPPIVSNPNFMEGLKYHEYNPRIISEKSKYLEDKICKECETFPLWLDFTGKKFWIDNFSEIISNLSKGVKIINEYPIIIKDKKIITNKGRSVIFDKIYNTISRKELFKLVGYSDPNLKSVSAFITILITKGEKEWDVYINGDTGIVFSHIIRKNIESDIFVNYIYSFFTSRLPDIKKVFSDLKRTHIFSRDEILAYRSHVIKDAILYGTPSIELDFIKNCGRLGLWKNISLEEAVDLVRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J0</accession>
    <name>F4B8J0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776857.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J0"/>
    <dbReference type="STRING" id="933801.Ahos_2071"/>
    <dbReference type="GeneID" id="10601571"/>
    <dbReference type="KEGG" id="aho:Ahos_2071"/>
    <dbReference type="eggNOG" id="arCOG05944">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500221_0_0_2"/>
    <dbReference type="OrthoDB" id="34590at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="179" mass="18769" checksum="7FEA6999F3AD757A" modified="2011-06-28" version="1">MPLSKDDKRKLIYAIVFSVASYAVAAGLALGTAAYALSIFTDPVVTLTIPLIIVAIGIQAINAKNGPLLILVISALLYAISGLIFLVPTFVVAGLVVEGVSRIVGYRSFKAVMINTTLAGGLVGVFSVVFGVLFVPSTASQFALDLMLAFTGIYFVESAVMGVISYYLGNFLIKSGVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K2</accession>
    <name>F4B8K2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AIR synthase related protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2083</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776869.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8K2"/>
    <dbReference type="STRING" id="933801.Ahos_2083"/>
    <dbReference type="GeneID" id="10601583"/>
    <dbReference type="KEGG" id="aho:Ahos_2083"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041631_0_0_2"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06061">
        <property type="entry name" value="PurM-like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="34"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="156"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="335" mass="37751" checksum="E679E74FD9C2C634" modified="2011-06-28" version="1">MLGKIDRKTFEEVIYPHLGEIHDEVIIPPMNGVDTGAIEIDERRVIVVKTDPVFIVPQFGFKKAAWFAVHILASDLMTSGIPPRYAMIDLNLPPSMTDEQFKEMWIGMHEAMKEIGVMVTAGHTGRYEGVDYPMLGGFSMIGIGEKEKLGSPKKVREGDVVIVTKGPAIEATALLVNLYPQYFKERLPSDIFKEAYDMYWKMSCWKDGLIASKIGIHLMHDATEGGLWNALVEVSEVTKKRIKIFEDKLFINRAVKEVTSLVNIDPWISISEGTMIIITDKAEVKDALQKEGIEAEIIGKVEEGEEGVYLGNKRIEKPTEDPFWRAFFDLQKKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N6</accession>
    <name>F4B8N6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carboxyltransferase subunit of acetyl-CoA carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776903.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N6"/>
    <dbReference type="STRING" id="933801.Ahos_2117"/>
    <dbReference type="GeneID" id="10601617"/>
    <dbReference type="KEGG" id="aho:Ahos_2117"/>
    <dbReference type="eggNOG" id="arCOG02705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_2_2"/>
    <dbReference type="OrthoDB" id="30579at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000016">
        <property type="entry name" value="Propionyl-CoA carboxylase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051047">
        <property type="entry name" value="AccD/PCCB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43842:SF2">
        <property type="entry name" value="PROPIONYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="269"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="522" mass="57297" checksum="94AE3A30127001AB" modified="2011-06-28" version="1">MSQEEKTMDKLIQELKNMKEKAYQGGGEDRIKAQHSKGKLTARERLALLFDEGTFNEIMTFATTKATEFGLDKMKMYGDGVVTGWGKVDGRTVFAYSQDFTELGGTLGEMHANKIAKVYELALKVGAPVVGINDSGGARIQEGAVALEGYGQVFKMNVMASGVIPQITIMAGPAAGGAVYSPALTDFIIMIKGDAYYMFVTGPEITKVVLGEEVSFQDLGGAVIHATKSGVVHFLAENEQDAINIAKRLLSYLPSNNMEEPPFMDTGDPADRDVKDVEQIVPTDSAKPFDMKEIIYRIVDNGEFLEVHKHWAQNIVVGFARIAGNVVGIVANNSQYLGAAIDIDAADKAARFIRFCDAFNIPLISLVDTPGYIPGTDQEYKGIIRHGAKMLYAFAEATVPKITVIIRKSYGGAHIAMSIKSLGADLVYAWPTAEIAVTGPEGAVRILYKKEIQASSNPDEFIKQKIAEYRKLFANPYWSAEKGLIDDVIEPKDTRRVIVSALEMLRNKREYRYPKKHGNIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T3</accession>
    <name>F4B8T3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8T3"/>
    <dbReference type="STRING" id="933801.Ahos_0917"/>
    <dbReference type="KEGG" id="aho:Ahos_0917"/>
    <dbReference type="eggNOG" id="arCOG05973">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369558_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="206" mass="23882" checksum="C9A9019B98752323" modified="2011-06-28" version="1">MNSELGKIFIFIHIYAIMKVGLLEDFKGNMTALWDAENLVIINGKEEEISLAYGRISNFMDLDILIGKNFLDGEINFLRQVYDIIIDGSNIAKKINIENFKAELTLGNVVISSARIFDDFVTFFFPFPIRNCSINYSIVYEVLDYDIMRELHRITKLGGKVRIIIKDKLHGGLSVDDIMKFIIKFDVEKISWKDGFWVIDLIKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W2</accession>
    <name>F4B8W2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W2"/>
    <dbReference type="STRING" id="933801.Ahos_0946"/>
    <dbReference type="KEGG" id="aho:Ahos_0946"/>
    <dbReference type="eggNOG" id="arCOG03869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159101_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="15556" checksum="0227D2749732BD3C" modified="2011-06-28" version="1">MGKDIFRLIPFFIVMNGTTVKQNMLQWAGMLLSTLWAGVHLDLVDAKLPNPTATLIYRVFFGFVASLAIVAAVALIQGIKKLYLPITIFYVIDFALLLETRTAPALFVGKVLPFNPYVDISLIIDAIMIVLSLALWKISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X1</accession>
    <name>F4B8X1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775757.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8X1"/>
    <dbReference type="STRING" id="933801.Ahos_0955"/>
    <dbReference type="GeneID" id="10600427"/>
    <dbReference type="KEGG" id="aho:Ahos_0955"/>
    <dbReference type="eggNOG" id="arCOG07326">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369550_0_0_2"/>
    <dbReference type="OrthoDB" id="33140at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="199" mass="23276" checksum="06304D2C2E3B1B79" modified="2011-06-28" version="1">MELKIDLPEDLVIPPLEEFSYICNGEVTKVKCNNTIYRDEDKIIATPYDIIYSISLENLVRNKTRGRKYNRWSYYVNKYKLTIEPIEFSIIIRTGAIISIFVDGLDINGISGDVIIKEFKISGSRDYEKIIDKLQDVNPRLIVVKKESFVFTISAYKVVYIDKNLQNIMKEFIGYKRMDCEKLVIKDYTRICYISSTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z9</accession>
    <name>F4B8Z9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z9"/>
    <dbReference type="STRING" id="933801.Ahos_2151"/>
    <dbReference type="KEGG" id="aho:Ahos_2151"/>
    <dbReference type="eggNOG" id="arCOG05959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500346_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="20110" checksum="25A7C4F5EE7B9968" modified="2011-06-28" version="1">MSNIRFPEPHIESTLSSIIKKEEPETIVLDGDTTQCYWDYECPRVIDVLYVIRSIAPWAQIIYVQGDMDPHAIKCIMAEPRYREEIIGTTMYIADVSSVKYYILHGHQGDIDQLRRSVGAGPWDWIIVGQYKRLEMDKLARVIYVGGITREFPPEARGYLVITDSNHYLRSLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B907</accession>
    <name>F4B907_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">succinate dehydrogenase</fullName>
            <ecNumber evidence="4">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_2159</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776945.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B907"/>
    <dbReference type="STRING" id="933801.Ahos_2159"/>
    <dbReference type="GeneID" id="10601662"/>
    <dbReference type="KEGG" id="aho:Ahos_2159"/>
    <dbReference type="eggNOG" id="arCOG00962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_1_2"/>
    <dbReference type="OrthoDB" id="144910at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000003">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009226">
        <property type="entry name" value="PRK12576.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="12" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="145"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="14">
        <location>
            <begin position="198"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE95030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="315" mass="36668" checksum="EDB8452598077F72" modified="2011-06-28" version="1">MSEEEKEIVVKIKRFSKEKGSWWQEYKLKVDRFTQMTEVLRRIKTEQDPTLAYRASCHMAVCGSCGMKINGEPRLACKTLALDMVRKYGKNEITIEPMDFFPVTKDLVVDWTDFYNRMFKVKPRLYPSKEVLEGKAEHRLKPEDQRELWKFEQCIWCGLCVSACPSVKNDPEFLGPAAHAKGYRFLADPRDTIFDERLKILIDSAWRCTYCYQCFNVCPRDIEPVTTIKKTRAYTKFLREKTPVALTGEKHAEAIVKSIEESGKIEEAKVYISTYGLLTAITDMIYAMQNGKLRYALVTQKKVKDMEQIRKIMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B992</accession>
    <name>F4B992_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775801.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B992"/>
    <dbReference type="STRING" id="933801.Ahos_0999"/>
    <dbReference type="GeneID" id="10600472"/>
    <dbReference type="KEGG" id="aho:Ahos_0999"/>
    <dbReference type="eggNOG" id="arCOG02114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062162_0_0_2"/>
    <dbReference type="OrthoDB" id="56850at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433:SF1">
        <property type="entry name" value="DUF1641 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25034" checksum="B4EBDF16B3F1BFC8" modified="2011-06-28" version="1">MIRTAAVEKLTSKIDEHKAEELSKFLDYLPTINDSLEKIKELKESGALDAIINFSYAIKSLKDALNDDSIQNLGNMLSTFMLLLSTTGEHANDVNQIISQASTINYTITKLKELKESGAFDVLVNFAYALKSLRDALTDDAITNIGTTLSLLLDFLPRAQEFLNYALNPSILKIMKALSSDEATKLLTNPPKVTLGGIVKQMTDDDVQKGIGVLFAIAKIIGKSYSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B997</accession>
    <name>F4B997_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GPR1/FUN34/yaaH family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775806.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B997"/>
    <dbReference type="STRING" id="933801.Ahos_1004"/>
    <dbReference type="GeneID" id="10600477"/>
    <dbReference type="KEGG" id="aho:Ahos_1004"/>
    <dbReference type="eggNOG" id="arCOG03176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051062_3_1_2"/>
    <dbReference type="OrthoDB" id="53209at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047622">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01114">
        <property type="entry name" value="GPR1_FUN34_YAAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="186" mass="19724" checksum="02E40556220038CC" modified="2011-06-28" version="1">MTEEKRANPAPLGLSGFALTTLVLSTYNAGLITSGSGVVLGLAAFYGGLAQLLAGILEWKGGNTFGYVAFFTYGAFWEWYFLTALGIFGNITAQGVGLVLIAFGIFTLIMWLATFKTNLGLFATFLLLWITFFLLGIGSMTCNTLLVHAGGYTGILTAIAAWYTGLVQVVAESLNKKAPLGRAPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B9</accession>
    <name>F4B9B9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyrrolo-quinoline quinone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775828.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9B9"/>
    <dbReference type="STRING" id="933801.Ahos_1026"/>
    <dbReference type="GeneID" id="10600501"/>
    <dbReference type="KEGG" id="aho:Ahos_1026"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_566972_0_0_2"/>
    <dbReference type="OrthoDB" id="145878at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.480">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512:SF30">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="2">
        <location>
            <begin position="124"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="2">
        <location>
            <begin position="314"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="425" mass="46930" checksum="F6B58B754BD2E575" modified="2011-06-28" version="1">MKRELAVIMLILIFLIIIILHSFSFLLYYHGNQRQFSHSSNSKEIRNIYNLTVYEVSFATPYDRNTTLFEGVPSHVYFIPVKTGFFILNVTTAIVVPPSVYHCLLLVTTSGPMNLANRQLCSCQGCVYAINISNGEIVWFQKFNNQIMTQPIVINCIVIIGLGNNIFQNCHVRGTGDNAILALNATAGKVLWEYKTLGEDMPTPAFYNGKIIEANGNGQVFALNLQGEVCWCKNITSYDSMSSPLLVNGVVYFGSANPYIFWAINATNGNIIWYVNFSSIYPSLGGLDDSSPAYCNGIIVSAYTIKVNCSIKEILFAVNATNGKILWCIDEGCTKIPPNLESPPPTIFNGVVYHDSPVGILYAVNLTSGKIIWAFKTGFTVDNPEIYEGKIIVQNASGYLFVLSYQGNLLKGFQTPVMPGPVICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D6</accession>
    <name>F4B9D6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fructose-1,6-bisphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776997.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D6"/>
    <dbReference type="STRING" id="933801.Ahos_2211"/>
    <dbReference type="GeneID" id="10601716"/>
    <dbReference type="KEGG" id="aho:Ahos_2211"/>
    <dbReference type="eggNOG" id="arCOG01349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_5_0_2"/>
    <dbReference type="OrthoDB" id="58111at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="29576" checksum="4FC3129A2AB7B8CB" modified="2011-06-28" version="1">MINTLRKIGEEATKYLRELHEKKGIDEIIGYHQGDTTRRVDKFSEQYIFDLLDHSGYKFSFVSEESGSIFRENYEYIAIIDPLDGSTNYITGIPWSSVSIAIYKKIEEEKFLPYAGIVAEVFGNNIYSYDENGAYINSVKVDKRKPTDKIVLPYYNKDKINEAYSIISKVGNGIKIRTLGSASLDMILVCTGRAYLYFDIRGKLRNVDIASSKGFCEKLGIRSLDLKGREVTFSLKYVDIVPEIIVTSDSELQNSFSSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K0</accession>
    <name>F4B9K0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Medium-chain-fatty-acid--CoA ligase (AlkK-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K0"/>
    <dbReference type="STRING" id="933801.Ahos_2275"/>
    <dbReference type="KEGG" id="aho:Ahos_2275"/>
    <dbReference type="eggNOG" id="arCOG02623">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43845:SF1">
        <property type="entry name" value="BLR5969 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent ligase C-terminal" evidence="1">
        <location>
            <begin position="380"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="474" mass="55256" checksum="51E802B47953C7FD" modified="2011-06-28" version="1">MREMSSVLQEYEAIHKELRDEGFIRTDYIHSDKLWNPKIMTMKREDLEKLKTFRLKRIVKWAWDHSEFYRRFWKSKGFEPDMIKDWRDVVKIPILRKDELRKDLQSNPPFGTIMVPELARRIRFVGATSGSTGMPTFQGWGALELDYFEEGQARYLWTFADVKPTTVYANYLNMSGFYSWGPPLVETAMWRCGATAIAGGGETYFSWKNRHNLIFRLWKVDVLATTPWLHRLIGEEAVKEGWETPFKVLLLHGGAAAENTKKKLFAVHPNAKLAISVWGTTDGHMAIEVPNLPGQLVVWEDMEIFDIVDPKTDEPAAEGERGELIATLLNHFTMPLIRYSLGDYVRNEFLTDPDPTYGITHMRFAEPIPGRVEWMFKVKGKLLLPIYVEDAVNEIPDTTGMFNIFVYDNEMDKLKIRIETTRNPDRKYDEEARQKLAERIGLDKDDVEIEWVKPGETIWTGYKLQVFVDQRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K4</accession>
    <name>F4B9K4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777065.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K4"/>
    <dbReference type="STRING" id="933801.Ahos_2279"/>
    <dbReference type="GeneID" id="10601785"/>
    <dbReference type="KEGG" id="aho:Ahos_2279"/>
    <dbReference type="HOGENOM" id="CLU_2662101_0_0_2"/>
    <dbReference type="OrthoDB" id="372797at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="75" mass="8580" checksum="67EDECEE24E08970" modified="2011-06-28" version="1">MQKLSISLLDAIERVRKLKENHEVFPFIAGKVVSFMIKSPSGLEEITFVERGEEGVDVFYTSEKVFKEVMGENNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T3</accession>
    <name>F4B9T3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="8">FGAR amidotransferase II</shortName>
            <shortName evidence="8">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purL</name>
        <name evidence="12" type="ordered locus">Ahos_1115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775915.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T3"/>
    <dbReference type="STRING" id="933801.Ahos_1115"/>
    <dbReference type="GeneID" id="10600589"/>
    <dbReference type="KEGG" id="aho:Ahos_1115"/>
    <dbReference type="eggNOG" id="arCOG00641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_2"/>
    <dbReference type="OrthoDB" id="8251at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000004">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002290">
        <property type="entry name" value="PRK01213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="11">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="64"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="190"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="416"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="573"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="703" mass="76506" checksum="EF20788DF5909591" modified="2011-06-28" version="1">MTISLSSYELELIKKELKREPKEEELKVIDALWSEHCSYKSSKVFLRSLPSEGPNVVMGIEDWQDAGAVDIGDGYAIVMKVESHNHPSAIDPFNGAATGVGGIIRDIISKGARPIALLDMIRVGSLRHERNRWLLKGIISGIGFYGNSIGVPVVGGELSFDDSYTDNPLVDVACVGIVRKDKIIPSVVKKPGLKLVLAGLTGIDGLGGASFASRKLSGEDEIGAVQIADPFAGKIILDVTLEIADKVEAIKDLGGGGLAVAVTEMANGLGAIVNLDKVPLRVKGMKPEDIIISETQERMLYAVKDENVKEVCKAFEYYEYPCAVIGEIIEEPKIKFLYEGKEVVSLPSSLLLSPPIYVWDMKENGEQKTGEKPNVRIEDAIRSIVTSEDLVSKEWAYSQFDYEVGTSTVIKPGEADSSLLLLPNGKFLAIKGDANPDLCVEDSYECGKAIFAEAYRNLATVGARGAAAVDHLQFGDPKKPKVYAKFVEAIRGISEAARFFNIPIVGGKVSFYNENSKGEPIKPTPLIVMAGLSDKPFRPSIEEGLDIVLIGITRDEMRASLFTKIFGQYGKLPKARLSEDLISSEIVIQGIHEGKITYAKDISKGGLIGALWSIITKGYSVSISTDVITTAEDIYSKLFSENGGRFIVLTNDSQFFTKLAEKKGTIARVVGKVTKGSGIYIDSDYYDLSKEIDIYNNYLEARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W4</accession>
    <name>F4B9W4_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777095.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W4"/>
    <dbReference type="STRING" id="933801.Ahos_2309"/>
    <dbReference type="GeneID" id="10601817"/>
    <dbReference type="KEGG" id="aho:Ahos_2309"/>
    <dbReference type="eggNOG" id="arCOG05323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1323954_0_0_2"/>
    <dbReference type="OrthoDB" id="33364at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="214" mass="24188" checksum="53C6A38938D9471A" modified="2011-06-28" version="1">MKPLLLGVDGLSYSSFMKCNPRFLLTLFSSAFRGVVVNRKPQHPASSWMSVLEMRDISGNSFYIKVEEKPTLIKETNAVAINLPITNPTYGELGFTYDSNLTVKDEIDKVIEAILDSLEEQPVIADITAIDRLVRVNPQAKCEIYKEVDDGLRKLLRKVDDFILFSPYGEPKSDKMCDHEDYGVYLSTVPRPNEHDTVKLPEIGLLFKRLASQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W9</accession>
    <name>F4B9W9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2314</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W9"/>
    <dbReference type="KEGG" id="aho:Ahos_2314"/>
    <dbReference type="eggNOG" id="arCOG03765">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406031_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="198" mass="22597" checksum="88A849B3698D4975" modified="2011-06-28" version="1">MSKVARVNIAADAEIVKELEREAKSRGYTIFSLTNIALKSMLELIRSGEDSTTLSHLVEFYKITKDLDIIPVTSWYIENLVKLAYEKDQKALEEICDGAGQQISSYLRSRASTFEELLQIYDTIKSVLPIKDIRIKRVNDEIIEIRITGSGFSSESTLCTSKVFRKILEAYYFEILEMTYSPGGIIFTRVKYQGQPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA15</accession>
    <name>F4BA15_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="6">OPRT</shortName>
            <shortName evidence="6">OPRTase</shortName>
            <ecNumber evidence="2 6">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pyrE</name>
        <name evidence="8" type="ordered locus">Ahos_1128</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA15"/>
    <dbReference type="STRING" id="933801.Ahos_1128"/>
    <dbReference type="KEGG" id="aho:Ahos_1128"/>
    <dbReference type="eggNOG" id="arCOG00029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_2_0_2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="7">
        <location>
            <begin position="52"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="125"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="23409" checksum="6D1FBAE5FBE91783" modified="2011-06-28" version="1">MRKLYLNSFSIIPRMDFAESLLQRKLLLIGNFILTSGKTSPYYLDLRRLPNYPEFFTVVDKAIEELRNVKFDMIIGIATGGIPLASFVACKLGKPMGYVRLEKKGYGTDKLLEADVKDKEIVIVDDVATTGGSIERSADEIIRNGGKVSHALVIVDRKEGAKERLEKMGITLHYLYSIHDILKSILPKLNDNERKMIEEYLVKNVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA24</accession>
    <name>F4BA24_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1137</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775937.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA24"/>
    <dbReference type="STRING" id="933801.Ahos_1137"/>
    <dbReference type="GeneID" id="10600611"/>
    <dbReference type="KEGG" id="aho:Ahos_1137"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="156" mass="17736" checksum="40567CE6EF80C30F" modified="2011-06-28" version="1">MSDSVKKKVDMDTIDRRLLIELLRDARSSLRRLSEEMNVSPATLHNRLTRLVQEGIIKGFTALIDYSKLGYTLSAVIMAKVDGKHLVEFEREVANADNVVAVYDVVGEYDVVLIAKFRSVEDLDAFLKQLLKNPKVERTYTSIVLNVVKEDPRVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L1</accession>
    <name>F4B3L1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774817.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L1"/>
    <dbReference type="STRING" id="933801.Ahos_0005"/>
    <dbReference type="GeneID" id="10599445"/>
    <dbReference type="KEGG" id="aho:Ahos_0005"/>
    <dbReference type="eggNOG" id="arCOG05511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353057_0_0_2"/>
    <dbReference type="OrthoDB" id="42703at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="11014" checksum="F14AEB5439938988" modified="2011-06-28" version="1">MKLSSRALEKLKSYGNYSISENGNDIIISYVTPSLLDASSIEGEDFRIVEIHCKKDNEGNLQILYAEIKNESNEVIRKMNLDELEPWIEYLESSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P2</accession>
    <name>F4B3P2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P2"/>
    <dbReference type="STRING" id="933801.Ahos_0036"/>
    <dbReference type="KEGG" id="aho:Ahos_0036"/>
    <dbReference type="eggNOG" id="arCOG01463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_843639_0_0_2"/>
    <dbReference type="OrthoDB" id="37087at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052902">
        <property type="entry name" value="ABC-2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43027">
        <property type="entry name" value="DOXORUBICIN RESISTANCE ABC TRANSPORTER PERMEASE PROTEIN DRRC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43027:SF1">
        <property type="entry name" value="DOXORUBICIN RESISTANCE ABC TRANSPORTER PERMEASE PROTEIN DRRC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="98"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE92931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="330" mass="36778" checksum="C320AD70C89B9247" modified="2011-06-28" version="1">MRNVIHTAKAIIKDNLGNKTTIFFILVFPLFLAIVFAFGFGGMNHVTQVVATNNEQLAKYLNSTQLFIGITCNNEKEAILHNYIYVNVQNNSLFNVTYPKDSKYLIPSLEAILTEYSTHDSAKVTTTQLPGFTYVDYILSGMIGVIALSNGVFGVTGVGAGYYRDKLVERLAASPLKSYEWVISLMLYEIIITFISIVPVLLLGVVLNFIPIIGILFVIFLIIGTLMFSGLGAIIFGLTPKDKLFIANIAANVVTLPLIFLSNAFFYVSSFPSFIEILINYQPVSVLNDIIRQVAVYQQLPQLWEIIYIIIFTVLSVFLGGKLLKLREID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P4</accession>
    <name>F4B3P4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PurM-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774850.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P4"/>
    <dbReference type="STRING" id="933801.Ahos_0038"/>
    <dbReference type="GeneID" id="10599478"/>
    <dbReference type="KEGG" id="aho:Ahos_0038"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041631_0_0_2"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF4">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="27"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="140"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE92933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="316" mass="34679" checksum="79048D162B712448" modified="2011-06-28" version="1">MSFGKVPLKEFLHKVPSGNCEICPSVGEDDAIIKIDKPYIIIHSDPITESSVDPGFLSIAVACNDVNMKGAPCKWALTTLLLSSRESLDRILSGINEACKILGCNVVGGHTEVVMNLPSDIVVTTAFSTTDKILTARNAKDGDYIVMVGSAGIEGTWILANQFEDYLLKLGVKKETIESAKRFKYDIIVQEKALNIVEYAIGMHDATEGGVLQAILEIAKLSNLKAVINPNLIPVRKETLEITRALSIDYLRLISSGAFLVITRNPEEVVNKVEESNVIGRLIRGEPSLHLEGVGDFNEDFEEELVRFESSYNGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P5</accession>
    <name>F4B3P5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved archaeal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P5"/>
    <dbReference type="STRING" id="933801.Ahos_0039"/>
    <dbReference type="KEGG" id="aho:Ahos_0039"/>
    <dbReference type="eggNOG" id="arCOG01871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098907_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF86">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="2">
        <location>
            <begin position="13"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE92934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="182" mass="20287" checksum="ED488C38518C8F9E" modified="2011-06-28" version="1">MRTLKKSLSDLKVVIMAGGKGSRLSPIKPVIEVCGKPMILWIYEFSKQFSDRIFIATVKDHPALPKLKEIISTNSIIFTEGKGYEFDVKEAVEKVGLPCLVFPSDTPFIPKGAIEKLINECETSICTLLSKGEFVGISLWNSLDTSSFSSINTDYEIDNVNTNKDLLKINEKCSEGFIWGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S0</accession>
    <name>F4B3S0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl10e</name>
        <name evidence="5" type="ordered locus">Ahos_1224</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776023.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S0"/>
    <dbReference type="STRING" id="933801.Ahos_1224"/>
    <dbReference type="GeneID" id="10600700"/>
    <dbReference type="KEGG" id="aho:Ahos_1224"/>
    <dbReference type="eggNOG" id="arCOG04113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="OrthoDB" id="30538at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_uL16_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_uL16_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003236">
        <property type="entry name" value="PRK04199.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="20143" checksum="459BF49BE1DDA777" modified="2011-06-28" version="1">MPLRPGRCYRHFSGPAYTRKEYIPGVPMPKITKFTMGTANKDYDYEVRMIVKELGQIRHNALEAARVMALKRISKLVGNETDFFLWVLKYPHHVLRENKMMAFAGADRLQDGMRLSFGKPIGTAVRIVKQGDVLMILRVKKEHLAFAKEAFEVASKKLPLDVEIVITPLKQEAKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U5</accession>
    <name>F4B3U5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776048.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U5"/>
    <dbReference type="STRING" id="933801.Ahos_1249"/>
    <dbReference type="GeneID" id="10600728"/>
    <dbReference type="KEGG" id="aho:Ahos_1249"/>
    <dbReference type="eggNOG" id="arCOG00551">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709217_0_0_2"/>
    <dbReference type="OrthoDB" id="34038at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059062">
        <property type="entry name" value="Gins15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059061">
        <property type="entry name" value="Gins15_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25865">
        <property type="entry name" value="Gins15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25864">
        <property type="entry name" value="Gins15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gins15 N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Gins15 C-terminal" evidence="2">
        <location>
            <begin position="100"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="148" mass="17170" checksum="51A93AA975589FFC" modified="2011-06-28" version="1">MLDEILNREISSETPEEISLSEIQKISQTLAKIRISYDDEIHKNEIKVYEELAESLFEVRLSKYLEDNYKGKGFDEFVFNILDKIKELYISLLSGNLIFNDGKILCKVMKDTIIENIELKKGDLIFLDLQKALALWTAEYITPCKIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V9</accession>
    <name>F4B3V9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallo-beta-lactamase domain protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776062.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V9"/>
    <dbReference type="STRING" id="933801.Ahos_1263"/>
    <dbReference type="GeneID" id="10600744"/>
    <dbReference type="KEGG" id="aho:Ahos_1263"/>
    <dbReference type="eggNOG" id="arCOG00506">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066633_0_0_2"/>
    <dbReference type="OrthoDB" id="6433at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07709">
        <property type="entry name" value="flavodiiron_proteins_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041">
        <property type="entry name" value="HYDROLASE, METALLO-BETA-LACTAMASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="36"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="29529" checksum="52605D54D327CA7F" modified="2011-06-28" version="1">MSVNLKVHKPYVLYEEGDHKFVWLGLDESEHEKGILTNQYLIVDGDEGALLDAGGYFVFERVYENVKEFIKPENIKYLLFSHQDPDVIGSLNLWLDVAPNAEIYVSELWERFLPHLGFEAAGKIIDIPDQGMSIKFGKSEIKAIPAHFMHSVGNFHYYDPVSKIYFSGDLGAAVFPAGKWYLFVENFDEHVKYMEPFHRRYIVSKKAIDHWLEQIKGLDIKIIAPQHGSIFQGENVKKFIEWLKSLDKVGIDYIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z1</accession>
    <name>F4B3Z1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin iron-sulfur binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774872.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z1"/>
    <dbReference type="STRING" id="933801.Ahos_0060"/>
    <dbReference type="GeneID" id="10599501"/>
    <dbReference type="KEGG" id="aho:Ahos_0060"/>
    <dbReference type="eggNOG" id="arCOG07320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_468237_0_0_2"/>
    <dbReference type="OrthoDB" id="42878at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF2">
        <property type="entry name" value="HETERODISULFIDE REDUCTASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="273"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="315"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="619" mass="70498" checksum="1428201E7812136F" modified="2011-06-28" version="1">MESLESLFYSSITSKNIDKYIKITREEDIRNLPNNVEAKYELLRGNVTSNIVADLSELKGVEDLGDSIKVLAGTKWRDIIGYSPELWSIMDFSVGGTISLGDEGFGYNEFGELANRVTVEAYLNGEKYTGKYRGGIIYAVYIKKENKPLIFKQYTGSEDIVLSKVKSLLSENVLPFRDISLIKDNEGTRLIVSYTQVREILVKRYIDGFSETFPPYPQLSSIGKFIYIGKTNLRNISFDLIKNVKYAYFTFRGNSAYYVVSSDIKLDITSISQEYSLEKFNGCILCGKCVDICPHSYQRGSPIFSPLGFFALSAIGKEAQVSNCHLCGICEEVCPVDLNIVDLLRQKFTLNSKVPNVQLTFPSKKSIILTAISDNLLEDVLKLIKYFSLRGVKLGVITLPDPLDKVVKGQINLENVRKLLENVDEIITLTPEEARYLIVLKSVKILDITFAYSMLENIVKPLMEGKKVHFPCFYSENKFYGCSYEMLNLANNEGYGENKVNAEITLCPLAAKRLGIKSYIDLLNINLNISDVYKLHSEISETLSTLDRVFEDAEWYKEIEPKLYEKIFSEAIDKVLLNKNYFEILYYYLNMDKLEFKNESIKSLVKNEIEKLLRSSDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z9</accession>
    <name>F4B3Z9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0070</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774880.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z9"/>
    <dbReference type="STRING" id="933801.Ahos_0070"/>
    <dbReference type="GeneID" id="10599510"/>
    <dbReference type="KEGG" id="aho:Ahos_0070"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_2_2"/>
    <dbReference type="OrthoDB" id="57092at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052017">
        <property type="entry name" value="TSUP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30269:SF37">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30269">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN YFCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE92963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="285" mass="30478" checksum="EE941159FBEE0602" modified="2011-06-28" version="1">MLTGIPLFLSVLFSSLLAGYLGSLTGLGGGTILVPILTLYDGIPIEYATGASLISTIATSSGAASAYLKDKIINLKIGTSLQVATTSGAIAGSLTATYIYSHHLEPIIYVVFGIVLLGSVYPTIKRGKFEIPMNSDPDWSTKFFQMHGEYYDMALQKKVEYWGVRWVQGASVMFVAGYVSGLLGIGSGALKVIGMDWVMNLPIKVTTTTSNFMIGVTAATSSGIYWVFGYIQPLIAAATSIGVLIGAFIGTKTLVKLKNKRIRTIFTILLAFLGIQMILRGLGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B419</accession>
    <name>F4B419_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B419"/>
    <dbReference type="STRING" id="933801.Ahos_0090"/>
    <dbReference type="KEGG" id="aho:Ahos_0090"/>
    <dbReference type="eggNOG" id="arCOG01606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_2"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053400">
        <property type="entry name" value="2-oxoacid_Fdx_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041170">
        <property type="entry name" value="Oxoac_fdxalpha_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF16">
        <property type="entry name" value="PYRUVATE FLAVODOXIN_FERREDOXIN OXIDOREDUCTASE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="7">
        <location>
            <begin position="7"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="236"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE92983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="618" mass="68218" checksum="230AA92FCF1FCB8C" modified="2011-06-28" version="1">MIGGAQGLGVDTSATIFGNAVARAGYYLYGNREYYSNIKGRHSYFQVVFSEKPLHSISSTVNVLATFDAETIFQHFTEVTDFIIYDKGVEGTNLDMVRSMEPEIAEQVRELLSKNGLGDTVKDVISYLEKKGVKAIPINYLELLKRVADAYKLPLSVVERAKNIISVAASMSLFGFKLDYLKQAISSLFKNDLFIKFNTMAAELGYSLTENHYKLPEISVSKPRIQVDGNTISAIGKLAAGLRFQSYYPITPASDESTYIEANQNLDMIVEAGELRKGGAVVVQAEDELAAINMAVGATLTGARAATATSGPGFALMAEGISWAGMNEAPVVITYYMRGAPSTGLPTRSGQGDLKFALNVGHGEFPRIVIASGDHEEIFWDAVWAFNLAEKYQTPVIHIIEKTLANAYSVFDEDHILGKKIPIERGKIVKPSGDFFRRFEITEDGISPRAFLGEASMFYAGDEHNEEGHITEGTENRIKMYEKRMKKLETADKEIPEEQRVNVVGDGNIVLLTWGSPKGAIIDAMDELKDVMMIQVKMFNPYPKNLMRKLLQGKSKIIAVENNYEAQGAQILTENTGIFPTNYILKWTGRAITREEIIEGVKQIVEKGEKKVVLSAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B426</accession>
    <name>F4B426_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774907.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B426"/>
    <dbReference type="STRING" id="933801.Ahos_0097"/>
    <dbReference type="GeneID" id="10599537"/>
    <dbReference type="KEGG" id="aho:Ahos_0097"/>
    <dbReference type="eggNOG" id="arCOG01465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089201_0_0_2"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF6">
        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="27"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE92990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="270" mass="30296" checksum="393BB3D514066E3E" modified="2011-06-28" version="1">MEIKGIISKLYAFLYIRGFKVWSSYRTQVILTVLSWTLPVFTYYFIGTSLGNKVVQDIHVTNYTSFFVIGLAFQGYVSSVITTISQRIRNEQLYGTIEYYVLSKSGVLSFLIYSAIWGFTINTINAVIILTIGDLLGVRYEINILSTLVLTLLLISSTLGIAFMSAASTMIIKQGNPISFFFSTFTTLLGGVVFPVTIMPSEVTYISYALPLTWALNGLRNAMLYGYGIFQVLKCIEILLLFNIILIPLGLLMYSFSFKKAREKGTLGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B445</accession>
    <name>F4B445_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Trypsin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774926.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B445"/>
    <dbReference type="STRING" id="933801.Ahos_0116"/>
    <dbReference type="GeneID" id="10599556"/>
    <dbReference type="KEGG" id="aho:Ahos_0116"/>
    <dbReference type="eggNOG" id="arCOG06005">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022755_0_0_2"/>
    <dbReference type="OrthoDB" id="36892at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024191">
        <property type="entry name" value="Peptidase_M61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007963">
        <property type="entry name" value="Peptidase_M61_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040756">
        <property type="entry name" value="Peptidase_M61_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05299">
        <property type="entry name" value="Peptidase_M61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17899">
        <property type="entry name" value="Peptidase_M61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycyl_aminpptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="389"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="492" mass="56893" checksum="E641E43E4C2AC01B" modified="2011-06-28" version="1">MIFEVKPRNRYFEILAKGREGVVIFPTYVPGSYVIRELERNIVEIEGIRISKNRFYVKDNFRYLIYAVSKDQREAISTLDYIFINPPAVFPFQEINEEYCVKLDLPPNWKISTSLKKKNEMYCADNYDEFADSPIEASPYLKVLKIDDKHVVSTIDDLDVEMLRRIVSEADKILGDEDNYVFHFRRSDKELGGIEHSNSSAIVVSWDREKLATLFAHEYFHRWNVKRLIPADLKHNYEKEVYTSLLWFAEGFTDYMAVIISLRAGVLDKKDAVKYVANSLSKLTFPGAKRTSLAESSFTTWIKYYRQDENFLNSSISYYDGGLALALYVDLEMSKKGKRIDDLFKEIYHEKRIYSFDDLNRILKGKYEIDIEDLVYGSAAKILEQLKDDVNIEFTDRGKPYYGIMLKDKTIAYVEDNSPADLAGLIPGDQIIGFNGKSKLEVKDSTKLTIVREGRIKEVVVNTSPNPGHKIKVKIGKLEFEKEEGISDIEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B454</accession>
    <name>F4B454_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyridoxal-5'-phosphate-dependent protein beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776078.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B454"/>
    <dbReference type="STRING" id="933801.Ahos_1279"/>
    <dbReference type="GeneID" id="10600760"/>
    <dbReference type="KEGG" id="aho:Ahos_1279"/>
    <dbReference type="eggNOG" id="arCOG01430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_2"/>
    <dbReference type="OrthoDB" id="10138at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="2">
        <location>
            <begin position="17"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="295" mass="32958" checksum="A4FFB24998E2243C" modified="2011-06-28" version="1">MSKDLHVFEDPIELLENMWPTPLLKLKIGEDVWAKLEFYNPFSHSIKDRTAWFLFRQALESNANEIVEATSGNLGIALASLSAIFNKKFTSFIPARAPQSFKVMMKILGAQVIQAGKSTTELLPLVKQYSKSTGALHLDQFRNPLNYMTHYYTTAKEIDEQLEYLNKKPQRIIATAGTGGHLTGIAKYFKEKYGSDIEIIGVQPAEGERIPGIKRQDENSFVGLVKIDKMIDITRKEAVEGIVDVARSSGILIGISAGATVAAYKKLADDKTTVLVFPDDAFKYFMELEDIDSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B465</accession>
    <name>F4B465_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl18a</name>
        <name evidence="5" type="synonym">rpl20e</name>
        <name evidence="5" type="synonym">rplX</name>
        <name evidence="7" type="ordered locus">Ahos_1290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776089.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B465"/>
    <dbReference type="STRING" id="933801.Ahos_1290"/>
    <dbReference type="GeneID" id="10600771"/>
    <dbReference type="GeneID" id="42778344"/>
    <dbReference type="KEGG" id="aho:Ahos_1290"/>
    <dbReference type="eggNOG" id="arCOG04175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177460_0_0_2"/>
    <dbReference type="OrthoDB" id="191241at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001981">
        <property type="entry name" value="PRK00773.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="6">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="87" mass="10434" checksum="9BCEB34586A743AB" modified="2011-06-28" version="1">MEEIKVYMVKGTALFNESRFPTRQKFIKFVRALNEKQATEYIYAYFGSKNKIKRHNIKIEEIKEIPLDEVPDRRIKDIAKLDKIILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B474</accession>
    <name>F4B474_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="12">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">alaS</name>
        <name evidence="14" type="ordered locus">Ahos_1299</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776098.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B474"/>
    <dbReference type="STRING" id="933801.Ahos_1299"/>
    <dbReference type="GeneID" id="10600780"/>
    <dbReference type="KEGG" id="aho:Ahos_1299"/>
    <dbReference type="eggNOG" id="arCOG01255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="OrthoDB" id="7506at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.54.20:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000056">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="13">
        <location>
            <begin position="60"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="705"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="709"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="902" mass="103060" checksum="DE315A50C701CB42" modified="2011-06-28" version="1">MKVDENEYRLKFFLENHYVRKQCISCKTPFWTTDENRDNCADIPCTDYTFLDSNIGKLNLSVSEARKKFLEFFHKHGHTIIPPKPVLARWRDDLYLTIASIVDFQPHVTSGMVQPPANPLVVSQPCIRLEDVDNVGITFGRHLTAFEMGGHHAFNYPDKFVYWKDETVRLAKEFFTEELKIPEELLTFKESWWEGGGNAGPSFEVTVGGLELATLVFMQYQIVNGEYVPLKLKIVDTGYGIERIAWFTQKTPTAFHAIYGNLVYTFFKKLDQPYVDEEMLKTAARFAGRIDPDNPLTITHHREMVAKALGLKKEEVEKELTRAARVFQVLDHTKTITLMLADGLVPSNSGEGYLGRLLIRRALRVMKILGSDIRLKDLVLEQIEFWKEDFPQFLKNKDYILDVVEYEQEKYEETLQKVPSVASALAKKGNITIEDLIKTYDTNGIPPDLIAEELKKKGIDISVPFNFYALVARRHQSAPIKGEKEKAKLTPDVVEFAKKFEPTDKLYYKDQYMRSFNGKVLGVYKNFLILDKTTFYPEGGGQLGDSGIIKTKSGEEYHVIDTQKVSDVVVHVLDKEAKLNVGEEVYGEIDWQRRYRLMRHHTATHVILAAAKKLLGEHVWQAGAEKTPEKARLDITHYKMLTPEEIKKLEDMTNYVINDRRPVTAIEMDRMKAEMTFGVSIYEGGVVNKPTIRLLEIKDWDVESCGGTHVSNTSEIGAIKIINVEKIQDGVIRLEYVAGDMVSNYARGIEDKINKISEDLETSPEQVEIRLKRTLEEMKEIKETLSMYRNWYSSYVESKVIEEKVNGINLKILPEIIDEDIVKDLLRKLTSQGSKIIAVFIGKKTVDIASSKDLKLDELASFLRNKGGKGGGKGTYVSMMLEGKSREEIINDIKEGIERSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B0</accession>
    <name>F4B4B0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell division protein CdvC, Vps4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1335</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2014" name="J. Mol. Biol." volume="426" first="510" last="525">
            <title>The oligomeric state of the active Vps4 AAA ATPase.</title>
            <authorList>
                <person name="Monroe N."/>
                <person name="Han H."/>
                <person name="Gonciarz M.D."/>
                <person name="Eckert D.M."/>
                <person name="Karren M.A."/>
                <person name="Whitby F.G."/>
                <person name="Sundquist W.I."/>
                <person name="Hill C.P."/>
            </authorList>
            <dbReference type="PubMed" id="24161953"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2013.09.043"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS)</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="4LCB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.08 A"/>
        <property type="chains" value="A=1-365"/>
    </dbReference>
    <dbReference type="PDBsum" id="4LCB"/>
    <dbReference type="AlphaFoldDB" id="F4B4B0"/>
    <dbReference type="SMR" id="F4B4B0"/>
    <dbReference type="STRING" id="933801.Ahos_1335"/>
    <dbReference type="KEGG" id="aho:Ahos_1335"/>
    <dbReference type="eggNOG" id="arCOG01307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_2_2"/>
    <dbReference type="EvolutionaryTrace" id="F4B4B0"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02682">
        <property type="entry name" value="MIT_AAA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19509">
        <property type="entry name" value="RecA-like_VPS4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000313">
        <property type="entry name" value="AAA ATPase, central domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001054">
        <property type="entry name" value="ATPase, AAA family, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054951">
        <property type="entry name" value="cell_div_CdvC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041006">
        <property type="entry name" value="cell_div_CdvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="9" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="127"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="4LCB"/>
        </source>
    </evidence>
    <sequence length="365" mass="42043" checksum="43FC626207BEDE82" modified="2011-06-28" version="1">MLEEMARKYAIAAVRADKEGNRDEAITYYKKAIDVLTQIVVLYPDSPTRQAYEQMIDEYKKRVSVLENMLPETPQEDNSQKTDDELIMKEKPKVSFSDIVGLDDVKEALKEAIIYPSKRPDLFPLGWPRGILLYGPPGNGKTMLAAAVANEIDSYFIHVDAASIMSKWLGEAEKNVAKIFNTAREYSKKDNKPAIIFVDEIDALLGTYTSEVGGEVRVRNQFLKEMDGIMDKNENYMVYVIGATNKPWRLDEPFLRRFQKRIYVPLPDFNQRLALFKYYTSKVKLGNVDLQELAKMTEGYSASDIRDIVQSAHMRVVKEMFEKNLSEPREITMDDFKEVLKIRKPSVNQELLKAYEAWTEKFKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B1</accession>
    <name>F4B4B1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase</fullName>
            <ecNumber evidence="3">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1336</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4B1"/>
    <dbReference type="STRING" id="933801.Ahos_1336"/>
    <dbReference type="KEGG" id="aho:Ahos_1336"/>
    <dbReference type="eggNOG" id="arCOG01527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_1_4_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005739">
        <property type="entry name" value="TopoI_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004438">
        <property type="entry name" value="PRK05776.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01057">
        <property type="entry name" value="topA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF26">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPO_IA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="10">
        <location>
            <begin position="98"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="602" mass="69898" checksum="847DD4C7C87871FF" modified="2011-06-28" version="1">MFNLTGPSGFPVFEVEWKPLWEIDKKSYYTRKYLILLQRLCKYAKEYINACDYDIEGSVIGYMIIKNFGDLKKAKRMKFSSLTKEELIKSFNNLQPLDYNMVNAGIARHIVDWLWGINVSRALMNAVKDTSKKSVILSAGRVQSPTLIEVVKRHLDRETFVPLPYFKIELKIRLGNIINTVYLDQEFHNIDDVESLITRLKKDKLIVDKNILNKDKIIRPPPFNLGDLQLEAGRLFGLSPYRTERVGEELYLDGVISYPRTNSQKIPPTININEIVTGLSQRFGKLVDELNLMTKGKFIVRQGNKDDPAHPAIYPTGVIPKKLGKQESEVYELIVRRFLASISRDAEVTRQRIILEFEKEKLKLQLDFQKITYEGWLKIYPYHRFENEELVNVNEGSEVEIISIKPKLLLAKPSTRFTRVSLLKWMEDVKIGTEATRGKIIETLFQRKYVVTKRSYIYPTKLGIIVSEVLEKYFNELTDVKMTADMEDKLDEIIYGKKNKDEVINEVKDKISKYFEKYNEEKKIIGLELAKGLNLVAYNKCKLCDFEAMKDGLCKYHLIAKEKLDEAIKIWEDRTGYDIKMILKYIKGSKSTGKLISDLLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C0</accession>
    <name>F4B4C0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase (Flavoprotein)-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776143.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C0"/>
    <dbReference type="STRING" id="933801.Ahos_1345"/>
    <dbReference type="GeneID" id="10600831"/>
    <dbReference type="KEGG" id="aho:Ahos_1345"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066799_0_0_2"/>
    <dbReference type="OrthoDB" id="46008at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF20">
        <property type="entry name" value="HYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="324" mass="37252" checksum="90DB6F99E73F1A91" modified="2011-06-28" version="1">MEKIAVIGGGVSGSYLAYLLSNSGYDVTLFDIKEKYFKPCGDVVPNIYKPKFEWKIKYYIKNFAFYLDGERIYDVGYRNPKWIVIDKPGWINSMREKVNQVISTKADKSKFDLTIDAKGPYDMDRQVVYTTRALIETNEFSDEAILEFNTKFTGFFWIFPDEEGVLNVGAGFIENKNSKDLLLNYIKNKFKDYKILDLRGAPISIGSVKSKSLKIGEARGLVFPMSGEGIRPSAISAEIAYEAITRGKNFDEYLSYNLTKIERRIEIQRRLLDVYMKSNINARKFLLKTFLRNDILIDAFLEDKLDFEGILESARGIKYGSIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C1</accession>
    <name>F4B4C1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 13">Valine--tRNA ligase</fullName>
            <ecNumber evidence="2 13">6.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ahos_1346</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2 13"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C1"/>
    <dbReference type="STRING" id="933801.Ahos_1346"/>
    <dbReference type="KEGG" id="aho:Ahos_1346"/>
    <dbReference type="eggNOG" id="arCOG00808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000192">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009687">
        <property type="entry name" value="PRK13208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="15">
        <location>
            <begin position="27"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="16">
        <location>
            <begin position="623"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE94229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="816" mass="95213" checksum="453A55F799A212CD" modified="2011-06-28" version="1">MLSQEYVTKKMEEWPKHYNPAEIEPKWENLWLSKEYWEKVFRFDEKSNKPVFYIDTPPPFTSGELHMGHAYWVTIADAIARFKKLQGYNVLIPQGWDTQGLPTELKVQYKLGIPKENRELFLQKCIEWTNEMINRMKTAMIRLGYRPDWEDFEYKTYSPEYRKVIQKSLIDMYKMGIVKIKEGPVYWCPKCETAVAQSEVGYLEKEGVLVYIGFPLKAGGEIVIATTRPELLAATQAIVVNPTDERYKDLIGKTAIVPIFNKEVKIIADEAVEKDFGTGAVMVSTYGDPQDIKWKLKYNLPSTEIIDNKGRIKGTGILDGLTVPQAKQKMIEILKEKGYVRRIEKIKHNVLSHTERSDCLSPIEFLTKKQIYIDVLKFKDKLLEEYKKMQFKPSRMSYYLEEWIKSLEWDWNISRQRVYGTPLPFWYCDNNHLIPAKEEDLPVDPTKTKPPYEKCPYCGLPLKPVTDVADVWIDSSVTVIYLSGFYTNKERFAKAFPASLRLQGTDIIRTWLFYTFFRTLMLTGNVPFKQVLINGQVLGPDGSRMSKSKGNVVSPLERVNEFGADAIRMTLLDASIGEDFPFKWDNVRGKKLLLQKLWNASRLSYPFIHGKNISKPNDLHVLDKWILIKHKKFVKKAIEAYNSYDFYIIIDELYNYFWETVADEYLELIKYRLFNEDQSALYTLRRILKDIIILLHPIVPHITEEIYSRLFGDKISVLLEELPNVDDIEEDNEAEKIGDYVKKFNSMVRTSKIKNKMSIVTPVNVKLYASNEMIESIKKVENDVKITLKINSLEYIPSQDREEVIIEKYENQPMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R2</accession>
    <name>F4B4R2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        </citation>
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        <source>
            <strain>W1</strain>
        </source>
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        <source>
            <dbReference type="EMBL" id="AEE94292.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="167" mass="18830" checksum="01640B4C573CA9F7" modified="2011-06-28" version="1">MPYSQGKFCFPLEVKEIRKGDIIVVKPTSVKSNGVQLVLPSLSLISESCNRKIDSLIWVDGVRIHGNEEIIFDGGKFKVQGKIKVESPEFLPGYILKKLLDDKEILINSLQVDGIPIVSIENFPLIYIKRDTNSCLKIHVNSVNNPILELASLSLYYYISSEYSEEI</sequence>
</entry>
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    <accession>F4B4S9</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="Pfam" id="PF05317">
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="574"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="598" mass="66284" checksum="57029EDB1F1A784C" modified="2011-06-28" version="1">MRKIYILMVIILILSSSIYTISLVSSTILPQQYYEYIKICATQYTAFVYSVSSNCSVKVMVLDQENFNNFSSGYSYSCIVSQLGKSVINGVLLNPGTYYLVIYSPYGYSNVTYFYCEIPIELSNCSTHVSEYLTLQPFNTERILVHLSTLGSPSLLRIVGISNESVCYKVVNCKGETMFSSGEVTLTLNGTNPCYNVTLPQGEYYLCIYNPTFSSALVYFCYRLYPQYVNPFLRFSVLQGGNYNYAPTGIASFGVSNTSPYEIKFSSVAGYFNISCILAYNSSQNLVKPCEASLQLNAVLVVCNENNATQIYWPQDVLLFLTNESVVLYHDNVLNLTNPLASLSNSSITSQNGYVMPTVNDGVTQYYYGNYKCAPYFEYNSPFSGLLIMNESVEKGQGVLITMEVEVLQNGTSSVMQSETFDKILIHDPDVKNAYFVVNGKEYTPAGIYGSLGSFYDAELILGGGGNGEITTFEKLNGLLGLYYFNGSEYVTFPSYYTFGADTAEATSNAHVTLEQNGMIKISTGSPDYTYLGEVKTQALKVSPYVPQRSSESSSSPESNIATSSEMTSSMQSSILYIILALVFAIAIIVIFRRPKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U5</accession>
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        <submittedName>
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        <name evidence="3" type="ordered locus">Ahos_0218</name>
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    <organism evidence="3 4">
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93110.1"/>
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    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0218"/>
    <dbReference type="GeneID" id="10599658"/>
    <dbReference type="KEGG" id="aho:Ahos_0218"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_3_0_2"/>
    <dbReference type="OrthoDB" id="85513at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    <dbReference type="GO" id="GO:0051539">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="230" mass="25306" checksum="0AE6E86333EEDD4B" modified="2011-06-28" version="1">MEPLRQLAKDKLSGKRVYAVMSAKGGVGKSVISSLLALTIGNTTLIDLDIHTMAIPKLFGLEGKLHKVTKNGIEPFEVKTTKIVSLSGIVKDNYVILPGGNQSSIMESLIAYSHIDTNTVLFDLPPGLGDEILVLERITDFTPIVVTTPSKISKKVVGYLTRYLIERGKEPKILVNLAYIECGGKVVKPFGEYEGFGLPIDPNLEDYIGRIQDYEGIIKEKLKEFIKTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V2</accession>
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        <name evidence="1" type="ordered locus">Ahos_0225</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_0225"/>
    <dbReference type="HOGENOM" id="CLU_3194305_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="45" mass="4964" checksum="BA1A52FEF13A90F6" modified="2011-06-28" version="1">MQSLLILVKGFRTMEATDQTLDMDCFTDIIITDIAGSGKNLLVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W2</accession>
    <name>F4B4W2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Molybdopterin-Binding domain of MopB superfamily</fullName>
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    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0235</name>
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    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775044.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W2"/>
    <dbReference type="STRING" id="933801.Ahos_0235"/>
    <dbReference type="GeneID" id="10599675"/>
    <dbReference type="KEGG" id="aho:Ahos_0235"/>
    <dbReference type="eggNOG" id="arCOG01496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="23466at2157"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43598:SF5">
        <property type="entry name" value="DMSO REDUCTASE CHAIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43598">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin dinucleotide-binding" evidence="6">
        <location>
            <begin position="1003"/>
            <end position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1234" mass="138902" checksum="AE597604EE900283" modified="2011-06-28" version="1">MLKLNRRDFLKLSGITALGASLAYLSSKGSFINKLFTESQTNQETALEDQDVIAFTTCYMCLGRCTMQYQITANNLIRYAGGDVVGRVNNGATCPIGPTAAMHYLSPARMKYPLLRPPTAERGTGQFIRISWEDLYDILLNGDNAAFLQERGWKYGFIGLKQIYQCCPEQFVMVTGRDQYNPTENRYFAAMFGTPNEYEHGGFCAANVAIGAVYVAGSTYWEYGYFDDHHAKVLILAGATQDHFPTGFRRRITMVKERGGTIVLFQPDRQPNLGPISNIWVPIRPGYDGLLISSIIHELLRLHKQGMQQNPPMPYIDEEYLKWYSNAPWLVITNPDGTIDPPTASNVGLFLRVTNKNGEWTPAVMGSDGNIYAFTDVPWQNNVEPMLEYEGDVTVPVAPGSTQTITVHVKSAFKLLEDQFLVDDYAPENVADKVGYPADQITALAQLLGDTAMRSPVIIEVPGGWVDYLGRTHNEYIGRPVATYIMRGISAHTNGFTTTRLFMLLEALLGAIDTPGGYRAKYPYPRPIPDGDFWPSYEATPDPRARITQADVQSGEIPATGPNGESYVGTLGGININERVYNDGTVVVLSANKVHYPAVITSTTNPFVYTPDQLVIDENGRPLLIDRGYSWEFPFAQHRSYNITMWDAGFQFPYPVKFLLWHITNPYWDNSYDIDKDMRLALMKNPDGTYVVPFIGIVDTFYGNSVPFVDIVLPDTTFLERYGEHSTLDRPITTPDIVADNIEHPILPPLFDSRPFSDVEIELGYLLGLSAFVNPDGSPKYPNGFGDFLWKWQISPGTGMLIAGRGADGTECCKGSPNPNQVKLYTYPGKVPAYQYGNQNIYPGLQEFPVTNGELPSFVPKGTQKIGHAEGEYELPLNIRYLRHVNMYYLQWAHSVGLIAYVKPIITQIYSEPIARFRLAAYGMWKGYNAYFYYMYLKTGNQQYLQLAQQNANVPNDTYGQYIAQIKKIAFWPIPKWYEPLSWTPDGANPQEYALLTEHRHVTPWFYHHWENHNPWLRPLLRYAPVFMSTEDCKKYGITDFDWVVFQSWTGETARLMVVCDETTRPGVVWYWKARNIRPGVLAIPPNSPEATISSMFNDVYSIVMPPSMGGASPTDYNNLNKAALIGTQGPNPALLNFDPHTGKTSWGDLRLKIVAVLGKGNYTVSYGLSDPYSLAVASAPFNPPPNLPYLQYNAYKTPDEMGLNWYAVNQTYYQWQQQKQVIRPLSQQSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W8</accession>
    <name>F4B4W8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Twin-arginine translocation protein, TatA/E family subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0241</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W8"/>
    <dbReference type="STRING" id="933801.Ahos_0241"/>
    <dbReference type="KEGG" id="aho:Ahos_0241"/>
    <dbReference type="eggNOG" id="arCOG02694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42982:SF1">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="98" mass="11286" checksum="F97209653B4E3D79" modified="2011-06-28" version="1">MSIMLGSPSDWVIIIIVALVIFFGSSKIPELFRSMGKAVGEFKKGRLEAELEMQQMQQQNQQPQVQNQADKAAELERQIQELQKQLEQLKSQKQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z1</accession>
    <name>F4B4Z1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775072.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Z1"/>
    <dbReference type="STRING" id="933801.Ahos_0265"/>
    <dbReference type="GeneID" id="10599705"/>
    <dbReference type="KEGG" id="aho:Ahos_0265"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135601_2_0_2"/>
    <dbReference type="OrthoDB" id="38711at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18689">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="132" mass="15068" checksum="19FEFF8CFE3DA4A6" modified="2011-06-28" version="1">MRRKILFDTGFFHVYFSGLNEEAKKAVEEVYTGKSIGYTLDLNLAEFLYTYGKLKGIEEANVRLSLILKSPIKIVSTNKELALRAGELKVKYQNLSIVDCFVVAFAEKENAVIYTTDSEIKRVYKNTIILRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B552</accession>
    <name>F4B552_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1471</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776269.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B552"/>
    <dbReference type="GeneID" id="10600970"/>
    <dbReference type="KEGG" id="aho:Ahos_1471"/>
    <dbReference type="eggNOG" id="arCOG10438">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173769_0_0_2"/>
    <dbReference type="OrthoDB" id="34524at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="85" mass="10354" checksum="C397D07AC06B9963" modified="2011-06-28" version="1">MVKLINWKKAPREERIKAKRLLREDCYDVYIILLQNRKFVEYFKSHDLDSGEKLLIRKERKGNVMKEIKRLREEGFSIKLVIFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B559</accession>
    <name>F4B559_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776276.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B559"/>
    <dbReference type="GeneID" id="10600978"/>
    <dbReference type="KEGG" id="aho:Ahos_1478"/>
    <dbReference type="eggNOG" id="arCOG10437">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2839428_0_0_2"/>
    <dbReference type="OrthoDB" id="40519at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="61" mass="7198" checksum="0C560F0660596218" modified="2011-06-28" version="1">MNEIEKIRLIISILEEKMPEDCAEILDEKFKILLKEIKNKGIDKVIRDYYSDDGDIEIIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B571</accession>
    <name>F4B571_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="2 8">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1490</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="2 8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776288.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B571"/>
    <dbReference type="STRING" id="933801.Ahos_1490"/>
    <dbReference type="GeneID" id="10600990"/>
    <dbReference type="KEGG" id="aho:Ahos_1490"/>
    <dbReference type="eggNOG" id="arCOG02937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040369_0_0_2"/>
    <dbReference type="OrthoDB" id="275333at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000072">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="10">
        <location>
            <begin position="9"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="9">
        <location>
            <begin position="182"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="324" mass="36505" checksum="820BE68634F3F00C" modified="2011-06-28" version="1">MKLEGEAIAPSNIAIVKYWGKRNAELNLPLNSSISISLDNLYARTKVIFSEEFSKDEVIINGKKLSEKETLNYAGRVLNIFRKIYGKSLFAKVISTTNFPPSSGLASSAAGIAALVYASNEALGLGLTQKELSKIARIGSGSACRSTEGGFVVWEKGEREDGEDSFCYQIFPPNYWEDLVDIIAIVSDEKKEVSSREGMEVSTRSSYLMKCRLDFIKETFNDVIESIRKKDEEKFFELTMRHSNSMHAVILDSWPSFFYLNDMSFKIIRWVQDFGHAGYTFDAGPNPHIFTTKKHMKEVINFLQDLNLKYIITSPGQGPKVLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B577</accession>
    <name>F4B577_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775087.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B577"/>
    <dbReference type="STRING" id="933801.Ahos_0280"/>
    <dbReference type="GeneID" id="10599720"/>
    <dbReference type="KEGG" id="aho:Ahos_0280"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="13450" checksum="E3E761AB6F40F538" modified="2011-06-28" version="1">MLTKDSIEKLLKEADELLDRGDTLQASEKLYKAAEEAIKVISFKNSIRTILKVKSLGHWSSKMYFEAVDELESIYPGIKSLWISAWILRVEGFHEISLDQKSIKLLKIDVEKIVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B582</accession>
    <name>F4B582_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-galactosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775092.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B582"/>
    <dbReference type="STRING" id="933801.Ahos_0285"/>
    <dbReference type="GeneID" id="10599725"/>
    <dbReference type="KEGG" id="aho:Ahos_0285"/>
    <dbReference type="eggNOG" id="arCOG05412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_0_1_2"/>
    <dbReference type="OrthoDB" id="84443at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF209">
        <property type="entry name" value="GALACTOLIPID GALACTOSYLTRANSFERASE SFR2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="382" mass="44632" checksum="CF54C15C5E273CB5" modified="2011-06-28" version="1">MKFGFSISSFQFEELNQNSDWYLWLTDYINLSGKKVVEELPTKNFYLSRYREVHSLAVNLNASIWRMNLSWGRLFPSKDKVSQEALFKYKEILKDLKDKGFEVILCLNHFDLPLWIHNPIVARDSLLTQGPLGWYSSSTVEEFVKFSRFIHNNFSEYADLWCTFNEPNLLLTFAYLQGIFPPGISSRKAYETSLNNVIRAHQEVYNELKGEKVGIVYNIPAVQGSSAIENEIFEFLRKIDFDWLGVNYYTRLVTDDKGIPLEGYGPFCQFGSREGRDVSDYGWEVYPEGLTDVLKKVSSFGKPILVTENGVADEKDRIRPRFIIDHVNAIKKSRVNVEAYMYWSLYDNFEWNFGYKMKFGLYDINLNPRPSAFIFKELQEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A8</accession>
    <name>F4B5A8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Malate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0311</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775118.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5A8"/>
    <dbReference type="STRING" id="933801.Ahos_0311"/>
    <dbReference type="GeneID" id="10599752"/>
    <dbReference type="KEGG" id="aho:Ahos_0311"/>
    <dbReference type="eggNOG" id="arCOG00246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_2_1_2"/>
    <dbReference type="OrthoDB" id="2596at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00300">
        <property type="entry name" value="LDH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="144"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="116"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="304" mass="33651" checksum="6AFD29BD5E92DE8B" modified="2011-06-28" version="1">MIKASFIGIGKIGQTIAYSAMTRGIFDEVVLYDIIPEFPEKFEHGLRRALASLRIDTEVIGTNSIDDVTGSDIVVISAGRPRRPGMSRRDLFADNAKIIINKELPKRNPGAVYVMVTNPVDMMASVFAKYSKEYVISTGDQVESMRLRAYISKILHVPVSQVDGFVGGEHGEDAVVLWSTVAVNGKQVEDLPKEQIEKYVKSIPGDIIRVMGGTTWGPGTIIADIIKAIVLNENRVMSIAVPRQYEDEIIHISIPTVVGSKIGPTLEDHLSEDDRWHLIAAMKDYYSVYKDMLKSIKTEGEIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D2</accession>
    <name>F4B5D2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0335</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775142.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D2"/>
    <dbReference type="STRING" id="933801.Ahos_0335"/>
    <dbReference type="GeneID" id="10599776"/>
    <dbReference type="KEGG" id="aho:Ahos_0335"/>
    <dbReference type="eggNOG" id="arCOG03661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003442_1_0_2"/>
    <dbReference type="OrthoDB" id="35948at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10789">
        <property type="entry name" value="GH38N_AMII_ER_cytosolic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000010">
        <property type="entry name" value="Cytosolic alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="5">
        <location>
            <begin position="470"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="957" mass="111233" checksum="C39B151064D5E414" modified="2011-06-28" version="1">MRNDAEVLSRISYILANSFYDIKYFKWSKENENEFYVDINNNNGYFMIVINYEGSALVKVNSQPYYALDGYHNTFPLPEGKIRVNALFSPYKAFGQKVEINPGTPVIFRRNEDAYKLWAYLNATLQLAKNSQDYLREKLLKVLTETLSLVPFVSVSRDQLLLASKYWNDFPSHLLEFSQEMKYEKFHEGNYKEALTYLKEKLGELRETFGKAGELIGFAHAHMDTAWLWNFDETRRKVARTFSTVLNLMSKYDFQYIQSMALYYEWIKEDYPELFNKIKEKVKEGKWILGAGWVEFDANLPSGESIARQLLYSQEFYLSNFGKIAEILWLPDTFGFSAQLPQIMKLSGIKYFATHKVFWNDTNKFPYSVFNWIGIDGTSIPSIAFGNGKGGYNSEFTIDSVMSQWNNWKDKDQPMLYSYGYGDGGGGPTEDMLNFAEVINELPSLPKVKLTGGIPEYKPENSWSDELYVETHRGVYTSHSKMKYLHRRAECTLRDAEIWSTIAGRYDKEIVNLWKILLKDEFHDVLPGSAINEVYKIVYPELEGIISKANKIIDESIKAIAGEGDRLMFFNSLSWDREDVAVVNEELPNSQKVDESYLVIVKVPSVGYSEYKEVKYSPVKVDGLLMENDYLKVKLDENGSILSIFDKEENREIIKSPSKLIFYENIPGWADAWDIEKSYKDTYFEVKAYKYEIKERGPIRACIRFYYKFRNSEITQDVCLNANSRRIDFKTRTKIPDRELLLKAWYYFDLNSTEAVYEIPFGVIKRKTTTSNSWELAKFEVPFIKWMDIYEDDYGVAVLNDGKYGIAVERSNVGISLAKTPIYPDYETDSEENVFTYSIYPHKGNWISAEVYKRAYELNYPIRATKGKGGEKSFVSVNPRNIILEALKPSEDGNGIILRLYNVENNRGIGEIKLWKSPNTAYKTNILENEKLEDVNRNKDEISFNYRNYEIISIKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5F3</accession>
    <name>F4B5F3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775163.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5F3"/>
    <dbReference type="STRING" id="933801.Ahos_0356"/>
    <dbReference type="GeneID" id="10599797"/>
    <dbReference type="KEGG" id="aho:Ahos_0356"/>
    <dbReference type="eggNOG" id="arCOG00823">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_13_0_2"/>
    <dbReference type="OrthoDB" id="28233at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="80" mass="9365" checksum="C9019CFEE4181BA5" modified="2011-06-28" version="1">MRKEFILKIDDRGRITIPKEVRELLKSKRLKLKIEDGKIILEPVVLDVNKYYGIFKKDIGNEDIDEILNKALAEVLLNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G9</accession>
    <name>F4B5G9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="4">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1510</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776308.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G9"/>
    <dbReference type="STRING" id="933801.Ahos_1510"/>
    <dbReference type="GeneID" id="10601010"/>
    <dbReference type="KEGG" id="aho:Ahos_1510"/>
    <dbReference type="eggNOG" id="arCOG03656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02002">
        <property type="entry name" value="TPP_BFDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="3"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="8">
        <location>
            <begin position="183"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="371"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="493" mass="55246" checksum="A9D99FD6C93F15D9" modified="2011-06-28" version="1">MLGDKIFEILREFTDRIYGNPGTTELSFIKKIPPDFKYYLALQDGIAVGMAEGYHLGSEKLAVVNLHAAPGLSNALGFLYTAYMDRIPMVIIGGQQTSSHLSDEPRLYGDLTTISKPVVKASFEARNADEGVKFLIRAIKTSLTPPYGPTFLSIPEDLEDKEVNITARSFMHKVELCCREDEVKEVMDEINSFDKIAIVAGYEIDVFNAHDEMREFAEKINSPVFAEPFASRTPFETPHKLFAGDLPRRSSEINKVLEDFPAVLIIGGSVNNVLFPDDEVLKGKEVIEVTYDWIEASRRPWKTLVCNPRDFLKLAIKYAKPHAFSPVKVSPPRNQKVEEIMKLLYPRLGNYAIFDEAPSYREIIRSIVGYKRRLFFANRAGFIGWAIPASFGYSSAGGKALAIVGDGSFNYSFQALWSATKYGGIMKVLVINNQGYNSLKGWGNTNAEILSPITSPWKLASSYGFEGKEFDDYKKGIEWLMEGNTQKLAELKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I2</accession>
    <name>F4B5I2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I2"/>
    <dbReference type="STRING" id="933801.Ahos_1523"/>
    <dbReference type="GeneID" id="10601023"/>
    <dbReference type="KEGG" id="aho:Ahos_1523"/>
    <dbReference type="eggNOG" id="arCOG07311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987483_0_0_2"/>
    <dbReference type="OrthoDB" id="42442at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="5"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14509" checksum="943999C0EA204903" modified="2011-06-28" version="1">MKSDKILEYCKTPKSFTELKELTGLSDAGLYKALNKFLEEGLIRKTEEGKYVTTDKGEKAYNNQLKSLMEEGIWMEYYGIGEDKIREILKILKSVKGEFYLKASRSLDESLLEQLIILQELNRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M6</accession>
    <name>F4B5M6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776365.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M6"/>
    <dbReference type="STRING" id="933801.Ahos_1567"/>
    <dbReference type="GeneID" id="10601068"/>
    <dbReference type="KEGG" id="aho:Ahos_1567"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="284"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="368" mass="43680" checksum="8E1159F5A277E9DF" modified="2011-06-28" version="1">MKIALSDSLLVLENNYVKALRFTLFWLKENVPNPEEKGVLSKVHEELYTRLREEYNLPSKVAEDCYRDTLSIYKGWYNNPRRGRFPRVYKPTVWLTPKASYNVDFIAMTVRIASVGELSILGYPRNLKEYENWRMKEARLVIKNDNAFLKVVFEKPSKKVEPKESIAIDINMSEIVVGKDDKNYVRIPTRLEEVHHWKSLAESLQRKYPRRWRENKRILHRIHSFHLKARRIMEDFARKVGKWVVEIAEDFGANVIKLENLRNLIKNVDKLPKEFRDKLYLMQYRRLQYWIGWQARKHGMIIKFVNPSYSSVSCPKCGRKMEEVGYRWFRCSCGYENDRDVIAIVNLNGRGSLTLSTAPQMRDVNPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5X7</accession>
    <name>F4B5X7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776390.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5X7"/>
    <dbReference type="GeneID" id="10601095"/>
    <dbReference type="KEGG" id="aho:Ahos_1594"/>
    <dbReference type="eggNOG" id="arCOG07246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074747_0_0_2"/>
    <dbReference type="OrthoDB" id="41698at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="34988" checksum="4FCAA34DCF5A3486" modified="2011-06-28" version="1">MRLLILLLLVSLLSIVSFSASISVQYPKEAFIGSKISINFTLTQQEINSTAFPFITAGVREISEKPLILDGIGCSFAIFKINDSSGELIITFVGKDNTSLGWNPGIVVYGGKFNPHIPDGQPGSCDLLLTFTGRLLVFLKTGWVNPISTLPLIGSPVNGWINVTKPFNYTAVLEDVNGSIFVKYVILNGEKYVVDYQTPIPWNFTYVGVRIDPSTLTVDDFCVYFTSPHQPYVVYVNGKEYAKGCTNCLGEGSVTLTVSSPSMVVNISFPLQHEYRVITISAQRDADVHVEYPVTQYALLGISVVLVVVSLLIERKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Y5</accession>
    <name>F4B5Y5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyltransferase, FkbM family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776398.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Y5"/>
    <dbReference type="STRING" id="933801.Ahos_1603"/>
    <dbReference type="GeneID" id="10601104"/>
    <dbReference type="KEGG" id="aho:Ahos_1603"/>
    <dbReference type="eggNOG" id="arCOG01401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101256_0_0_2"/>
    <dbReference type="OrthoDB" id="40095at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRM5/TYW2-like methyltransferase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="28142" checksum="72FB9F65E371F598" modified="2011-06-28" version="1">MSLFRYGGLRIEIPKGYEYVYYSTFIVGEYDFLKFKKEDTVLDAGAFIGDFTVKIARKVKEVVAVEPLPWAFKILKRNVEVNELKNVTLVNKALYDEDGIKIRISDEGVGSKVSNEGIEVTTTTIESLGKFSVVKMDIEGTEGRLIKGNWLNSVREIAMELHGNENVISIPAMLRGKGFNVRFMKWSDVVKNSVKNISLHPLDFLKAEMKTWIMSNAIKGFLTRNKGYTIPSLTKEEFKILYGRRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B629</accession>
    <name>F4B629_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776442.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B629"/>
    <dbReference type="GeneID" id="10601149"/>
    <dbReference type="KEGG" id="aho:Ahos_1649"/>
    <dbReference type="HOGENOM" id="CLU_2519560_0_0_2"/>
    <dbReference type="OrthoDB" id="39461at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="10375" checksum="1B096A31FF6E2719" modified="2011-06-28" version="1">MDEKTYNEIIKPFGSYDRFKKRLEDIKAGIEFLKRTGKDPLEISFQLYEIWEKEYLKLYNNDVKKAREDLIREWRRKLRSLKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B675</accession>
    <name>F4B675_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775279.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B675"/>
    <dbReference type="STRING" id="933801.Ahos_0475"/>
    <dbReference type="GeneID" id="10599915"/>
    <dbReference type="KEGG" id="aho:Ahos_0475"/>
    <dbReference type="eggNOG" id="arCOG01200">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_1_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449:SF3">
        <property type="entry name" value="POLYMERASE NUCLEOTIDYL TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="11827" checksum="FF028F1A8C5BD61A" modified="2011-06-28" version="1">MEKVVENFVEKVKEKYEGKVTIVLFGSRARGDYWPSSDYDFMIFLERVEDKTKEAYEIYKLKRGFSADIIVLSKDELKDKIVEKMLEDKIVIYDGLGLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B696</accession>
    <name>F4B696_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775300.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B696"/>
    <dbReference type="STRING" id="933801.Ahos_0496"/>
    <dbReference type="GeneID" id="10599936"/>
    <dbReference type="KEGG" id="aho:Ahos_0496"/>
    <dbReference type="eggNOG" id="arCOG03239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_549365_0_0_2"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_GajA/Old/RecF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051396">
        <property type="entry name" value="Bact_Antivir_Def_Nuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581">
        <property type="entry name" value="ATP/GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43581:SF4">
        <property type="entry name" value="ATP_GTP PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease GajA/Old nuclease/RecF-like AAA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease GajA/Old nuclease/RecF-like AAA" evidence="1">
        <location>
            <begin position="189"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="496" mass="57684" checksum="F35772D75C3120DE" modified="2011-06-28" version="1">MRLLEFYTNNFRSLSDVSIKDIGGLNVVVGFNGYGKTNFLSSIYLFIKNLNAGIEKRSFEDNNQEYLLLWQGYDTSKEITLGGKIEFSEDEVSKAIGKRKRMEIEIVNKLKYERKEVKWDLDTIRINDSIPTRDEIDDVRRLFELASQQVEYVPIFDQGYFDNIMKRIIEISRSPINLRKYWYDFVNLVSATIPEIKGLEIWEGGKLVLNVYNLPIYIDLAASGFQRVILMLFVIWLSGNKVLLLEEPEVNMHPTLQYKVAKLLKGWTDNQMLQVFMTTHSPFIVSSDIDNYIILRRKEGGFSKAISVKPNEELKSMLSLLKVNLSDLLFSRYIILTGENAEPSAVYNWLKKMNIYPEYNGISIYAVKSDMELQSWLKLKEILNLDVIFFGLCDKLDQSIRDSCIPLSREIESYYTKTGILEALKLIGIYPDEKELRDLSKDDNLRWLINVMKKRGLDYYYLRSSISDIITRVDSVEIPKEVEVLGNKIKSMQTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A2</accession>
    <name>F4B6A2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0502</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775306.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6A2"/>
    <dbReference type="STRING" id="933801.Ahos_0502"/>
    <dbReference type="GeneID" id="10599943"/>
    <dbReference type="KEGG" id="aho:Ahos_0502"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_0_2"/>
    <dbReference type="OrthoDB" id="12267at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="303" mass="31655" checksum="D1B1BD09195F4417" modified="2011-06-28" version="1">MITIVVTPIEYILAIISGVLVGFSLGLIGGGGSILAVPLLLYFVGLATVPPQYASNPTLAHEYSDYVAHVALGTTALAVGLNAYINSYMHFRKGNVRLPEGIMFTIPGIAGATLGGYASHITPGQSLLFFFGILMIAVALLMLRPQKQTRTISKTQTAQISLRGLSALSIKSLTSDVSIKKVIPAGFIVGFASGYFGIGGGFLIVPGLLFSTGLCMIKAVGTSLISVGTFGITSAAVYAYYGYVLPLISIAYLVGGIAGGYAGASIASRMPRGMLRKVFAGIIIAVAIYIMYENINGLLVLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A5</accession>
    <name>F4B6A5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydrodipicolinate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0505</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775309.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6A5"/>
    <dbReference type="STRING" id="933801.Ahos_0505"/>
    <dbReference type="GeneID" id="10599946"/>
    <dbReference type="KEGG" id="aho:Ahos_0505"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_2"/>
    <dbReference type="OrthoDB" id="33636at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008747">
        <property type="term" value="F:N-acetylneuraminate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00408">
        <property type="entry name" value="DHDPS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42849">
        <property type="entry name" value="N-ACETYLNEURAMINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42849:SF1">
        <property type="entry name" value="N-ACETYLNEURAMINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="288" mass="32089" checksum="D7BB9B094994CC75" modified="2011-06-28" version="1">MKGLLTALVTPFNSSGDLNLDALDTLIKFDLSRGVDGFWVLGTTGEFNMLSIEEKMQVAKKVIDLAKGKVLLGVNENSTYNSLKLAKYFVDLGVNGIFSVPPIYHKPNEKGLIHYYEDLGKFGEEVYVYNIPSLVGYNIPLTVLQKLVEDGIIQGMKYTTSDFESLINYMRFLKNLNSKFEFLAGNDKFILISLLYGVDGIVSGISNFAPEVVSQLYKNVKEGKITEALKLQEIVDKLVEVTSLADYPTGIKIALRYRGIDVGSSRKPLEENITADSAIYHTLKEFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C0</accession>
    <name>F4B6C0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776455.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C0"/>
    <dbReference type="STRING" id="933801.Ahos_1662"/>
    <dbReference type="GeneID" id="10601162"/>
    <dbReference type="KEGG" id="aho:Ahos_1662"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061108_0_0_2"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="375" mass="42574" checksum="11025FD50ED356A5" modified="2011-06-28" version="1">MWFTYGPPTENPFDRDEEISTLIKLMRGGQPVSLVGPRRIGKTSILLASLKKSNLPYVLISAEDFLKGEKGFNFTEFLSAYVSKVILSLHPFSGYAFKLEEKVKSYLKVLRDLLGAVKISLNIPEVSGLIEVVLDKGEKGKDLSEEFSRALELPELLAEKFNIRIVIAIDEFQYLNFAKQAVPEIFHVMRSKWQFQKRVSYVISGSAVGMLKEIMSSKNQPFYQYFYSIRVNPFNRETSKEFLRKGFETDGVKVSEEDIEKIAEYVDGFPAWLNLVGIKVELEGEVEKALSSLPFDDNVISAIEGDLNKLSPTARSVLRKLAKLGGTGSPKDLGENEWSVQRGLKQLIRYGYVEREERGIYKIIDPMVTHYLSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D9</accession>
    <name>F4B6D9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Biotin/lipoate A/B protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D9"/>
    <dbReference type="STRING" id="933801.Ahos_1682"/>
    <dbReference type="KEGG" id="aho:Ahos_1682"/>
    <dbReference type="eggNOG" id="arCOG01939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050664">
        <property type="entry name" value="Octanoyltrans_LipM/LipL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679:SF2">
        <property type="entry name" value="OCTANOYL-[GCVH]:PROTEIN N-OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43679">
        <property type="entry name" value="OCTANOYLTRANSFERASE LIPM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="29"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="291" mass="32810" checksum="703A22CFA96F2DAC" modified="2011-06-28" version="1">MDSFRFLIERNSQAKILAGEEALLESVASGGESTLRFVIFDPTAVLVGYHQAVEQEVNVEEVKRRGWEIGRRPTGGGTIIMGPFQLGWEIYAESSIIGNPEIAIRKGAEGVIITLKKLGLNANFRPKNDVEINDRKISGIGAFSIGKYVSVTGTILLDFNVDDMISTLKLSSEKLKDKIAKDYKERVTWINRERSPIDMEELISTAKEGFEEALKVKLIDGKYTDKEIEDINILSLKYSSPEWIFNTRIPLKGDIKYAEEKFPGGLVKVQAKVQGKVIEAIFDYWRLFRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H2</accession>
    <name>F4B6H2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfur carrier protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fdhD</name>
        <name evidence="4" type="ordered locus">Ahos_1715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FdhD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776507.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H2"/>
    <dbReference type="STRING" id="933801.Ahos_1715"/>
    <dbReference type="GeneID" id="10601215"/>
    <dbReference type="KEGG" id="aho:Ahos_1715"/>
    <dbReference type="eggNOG" id="arCOG04358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_3_0_2"/>
    <dbReference type="OrthoDB" id="57189at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="FORMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN FDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="3">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="28116" checksum="FC546A7979514DD8" modified="2011-06-28" version="1">MQIKRVSLERIREEEEISGEDYVAVEEPMHLEICKDNCETFAIIMRTPGNDKELSLGFLYSEGVINGIEDVEKVVQKDKNTVEVWVNRLMKVKTRELIVNSSCGVCGRALLYTISILKSNLKVSKKLIFSLPSILRENQNAFNVTGGLHASALFDASGKLLKIYEDVGRHNAVDKIVGSLLIEKEIPAEKSILLVSGRLGYEIVSKGIKAGIPIIAGISAPTSLAVEIAEEAGVTLIGFLRGDSFNIYSHKERLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J4</accession>
    <name>F4B6J4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="11" type="ordered locus">Ahos_0516</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775320.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J4"/>
    <dbReference type="STRING" id="933801.Ahos_0516"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="GeneID" id="10599957"/>
    <dbReference type="KEGG" id="aho:Ahos_0516"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5005050842" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023566412" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="12"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="209" mass="23082" checksum="FF79D0DFCEEB8D94" modified="2011-06-28" version="1">METPKIKTWKGTTTVGILAKDGVVLAADRRASEGFFVANKMVRKILYITDNIGITTAGSVADLQFLYYVLKNLYHYNYISGNRPTTVKALATYLANTLSQNKYFPYLVQILIGGYDTQPRLYNLDYFGDMTEEKYVATGSGSPVAMGVLEDEYREDLTADQAMDIAARAVLSAIKRDSYTGTGVIVSKITKTGHVEKEVYLRKGAVNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T7</accession>
    <name>F4B6T7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776545.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T7"/>
    <dbReference type="GeneID" id="25395611"/>
    <dbReference type="KEGG" id="aho:Ahos_1753"/>
    <dbReference type="eggNOG" id="arCOG08454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2032743_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="121" mass="13937" checksum="00031DADC9DDAEBC" modified="2011-06-28" version="1">MKQLTGIENVEEIIKGLNILFGPFPKLTRLDLSLFYTVIKLPRRSFLRPVDFYYLLDGFITAALRDSLSSNTVLLSFVFPYDSGILCKFWIGLGRRVWLRIIGFLGSIGGRFFPLIFRLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U9</accession>
    <name>F4B6U9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OrfB transposable element-related protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U9"/>
    <dbReference type="KEGG" id="aho:Ahos_1765"/>
    <dbReference type="eggNOG" id="arCOG04197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064882_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="354" mass="40648" checksum="556DBEE419807AA6" modified="2011-06-28" version="1">MMRSKLIGSKESIENFQFVTINGKVEFENVERISRIAYYYSKAVKAGINLALKGVSLNDAVKELYKIIPYAFYAETAYKQALALVENKGSKVEIRKKWIACRGSKGDNGNRGIKFHVFQDHVEVRVKDPWGKWVHGKAYFGRKYLPLLSELKELSRRKEEGYGAVISFKHYPMIHLQIPLWLYLKHFSSLKPNGYGLFAGFDLNSDRLNVVVINKDGEIIAKKTWWYSDVTRPGFPKGKARALRLNALSESLNFLSRIGVDYVAFEDLFLVKNRRFTRSKSGNRKVLRFAKRQMLTHGVIKALRLGFNVVLVNPKGTTNSEYHDKIMREKGFDRHTASAYLIALKGLEVIKNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6V4</accession>
    <name>F4B6V4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6V4"/>
    <dbReference type="STRING" id="933801.Ahos_1771"/>
    <dbReference type="KEGG" id="aho:Ahos_1771"/>
    <dbReference type="HOGENOM" id="CLU_2839166_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="65" mass="7338" checksum="DED767ABCEC6D79A" modified="2011-06-28" version="1">MCVRPNSFQYVTNTPIYVVMCNRTVTTFEWFSRDNSYKVCTASASADSAGILMNVYLAYSNSNYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W9</accession>
    <name>F4B6W9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776577.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6W9"/>
    <dbReference type="STRING" id="933801.Ahos_1786"/>
    <dbReference type="GeneID" id="10601286"/>
    <dbReference type="KEGG" id="aho:Ahos_1786"/>
    <dbReference type="eggNOG" id="arCOG03672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_334538_0_0_2"/>
    <dbReference type="OrthoDB" id="34640at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="763"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="787" mass="87703" checksum="1C5C88DB7848FF5D" modified="2011-06-28" version="1">MRMLKVLLLALLVLPLFSFFTQSLSLYGQIQLPYGWLLPVYENVTQGNGIDIIFYASSPITFMIMSPLQFCQFRQGNSSQSIYSITASSLSEFFPLPSGQYYIVFYNNVSDTTVTLNYYVLTRPLPTGIADYGLKVSNGTVSPYIEKIKSVIGAVEINKLLAYNSTPPSGIGQYCASIQLNVVLQVNTISGTRQIWLQNVIQINTYNNSYRFIDNIWNFTSYPSILCNCVKGNGGVYFMFQGIRVCDYYAYSTNYFTLFSPSIEYLIINTSYTSQGPIILFGYMNQSGYPVWYDNVSILIPGTLSAYILIDGYNLTGNNHSYDAELILGGGGSGEFTFFNESNVELAMIYQYLNGTLAPPKYLFPFGLDTEESADNLYTIAYKGAYLVSTGYQVINNLNGNISQLRFNVVNYTKVTDQHFPYLFTINISGGVLPYKLNITIFNSTNELSAHTYVLFPALSSYYLPLSQLFPGNYTVKIELTDFNGNSIYYEFPLTINPPLKVRIINVTNFTDISIPFFNFTSILCGGTKPYNVTVMIYNGSGLLSEIHKILNCSSHFTINMKGYTEGIYTIEIKVEDQIGSFNISKYNFTINPDPEVKLIMPNTIDAGVEFQINANISLGTPPYHVSWYINGSYIGGGSTDKLNLTSPGIYNITAVVRDSVGYVITVSKIITIVPPPSLSVNEQTQGNFFQYNTSILLTTTVKGGTDAYYLVYLNGNLIGNYSSTTQMHLNLQNGENNITILAKDLWGKSAEETLMVNSGYNYINIGAVIAGIILIIIIIAIIVKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B711</accession>
    <name>F4B711_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NFACT RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">W1</strain>
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    </reference>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="566" mass="64932" checksum="4482D418E77A080D" modified="2011-06-28" version="1">MSYIDLLAWIVENKNELIGCRIDNIYNLEKLDNVFIFKLHCKNSDKNLIIEPGVRINFTKYDIQKEIGNKAKSLRELIRDLVISDVQIIDKERILKITLNNGMKIIVELLPRGLLLVLDEQDKIKFSTEYKEFKDRVVKPGLPYVQPPKPNKVVVPKDILFALHLTESVNAEEEYEKLEKSIAEGKITPCIQKGINFMPIEFEGCEKGNSFNDVIDEYFLELTKQKEIQKITEKIEGEKKKLEKTIQQIEENIKEYEEEAEELRKIGKEIMEHYMEVENAIKNKEKKIKLEGKEIEIDPKLSVAKNASLYFDKAKEYVQKSKKAKETLEELKRKLNEIEIEIKKEEEGRKLSIRKKEWYEKYRWSFTTNGFLVIAGKDADQNESLVRKLLEDNDIFLHADIQGAAATIIKNPKNITEQDIYDAAAIAASYSKAWKLGLAAVDVFWVYGSQVSKSPPAGEYLPKGSFMIYGKKNYIKSVKLNLAIGFKINDSLEIVVGSENSVSAKVKNYVLISPGDELERTADRIVKILSEANSINAGKELKNEMLSILPGKSKIIKVVKETNDKQ</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="933801.Ahos_0617"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>F4B784</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PaREP1"/>
    </dbReference>
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        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="155" mass="17765" checksum="DEC6281FC2E01941" modified="2011-06-28" version="1">MEELIKKAEEKGINVEDLIISALSKEDPQEGIKARLELAEKYMKEAEEFLTKGDVVQSSEKAYKVAEEIVKAFAEKFNLTEYQQAVKEDRWHTYTLANAAAKLSSKLGDWIKTGWNSAYTLHVWGFHEAKLDIDSVKNLLQDIKRMLEESKKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
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    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C3"/>
    <dbReference type="STRING" id="933801.Ahos_1870"/>
    <dbReference type="GeneID" id="10601369"/>
    <dbReference type="KEGG" id="aho:Ahos_1870"/>
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        <property type="entry name" value="Glyceraldehyde_dh-related"/>
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        <property type="entry name" value="glyceraldDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="727" mass="80224" checksum="2A60F798D5EF75D9" modified="2011-06-28" version="1">MYVGQRIRRKEDLKLITGTGRYVDDINLNGTLYLAILRSNIPHARLKKINVEDALKLPGVVDVITGLKISVENRPNNFPMAVDEILYEGQPIAAVLAEDRYTAYDALEYISYDYEELPAVVDPEEAVKDEVKAVEGKSNIAYSKVYKGGNAEKAYESSDVKIEEKFVISRVYPAPMEPRGLVVDFQQDRITVYASTQSPHYMRSYLLSAFPEYDFRVIQADVGGAFGSKLFPYPEDYITVYASIKSRRPVKFVNTRTEELHSTYHSRGQIHHIKLGANKDGKINAVIDDIIIDLGAAWHGFYLADISGTLITGPYDIKNATSTIKGVLTNKVPLDQYRGAGRPEAAFVYERMMDILADELGIDPIELRKKNAIRETPYVNPFGLKYDSGNYLDLLNKAEGFYREMEKTAEELRKQGKKVGVGLSFYIEQNNFGPWESASVRLLSNGKVEVIIGAAPHGQGTGTGIAQIVADELQINIDDVNVVWGDTDKISNAFGTYGSRSLTLAGNAALLAARRLKENIIKLAASFLQSDPEELQYKDGKVINPKSGKMMSLAEIARMATSNLGGTWNYKAEPTLEATASFGLDNYTFPYGSHIAMAEVDETGKVKILNYVAIDDIGVVVNPMLAEGQVDGGIIQGFGEVTMEEIIYDERGNLLTSSFAEYGIPSAVEAFNMKWLYMEEGKSNAPLPAKGIGEGATIGALPAITRAIEKAVGKRITKIPVRMEELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G7</accession>
    <name>F4B7G7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0685</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.14" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775488.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G7"/>
    <dbReference type="STRING" id="933801.Ahos_0685"/>
    <dbReference type="GeneID" id="10600137"/>
    <dbReference type="KEGG" id="aho:Ahos_0685"/>
    <dbReference type="eggNOG" id="arCOG00405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_2_1_2"/>
    <dbReference type="OrthoDB" id="6113at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000005">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000002">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="4"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="570" mass="65637" checksum="28317CA9384C9293" modified="2011-06-28" version="1">MSEEKVIELAKRRGIFWPSYEIYGGVGGLYDIGPIGTRIKNKIVQLWRKIFVEENSDFVVEIETPMITPKKVFEASGHLENFTDPIVECNKCHRVFRADHLVEEVLKISAEGLKAEELTNLIREKNIKCPVCGGDLGDVRYFNLLFSTNIGPYTGSTGFLRPETAQGMFTSFKRVYESTREKLPLGIAQVGRVARNEISPRQGLIRMREFTIMEIEFFMDPEDTENVPLDKFSEDKINVLPAEAKIKGDKPSTFKVKELVDEKIVINPWMAYWMATANKFVRKLGVSLERVYFEEKLPSERAHYSSQTFDQMVIVGEDKVEISGHAYRGDYDLSRHSKFSGQDLSVFKKYDQPKIIKKKIVIINKDKLNKESKDFVKEFMKRISSLKPEEVEKILNEKIDGKEIKEYVTVTEREEKESGKRFIPHVVEPSFGVERCLYISVLNAYREKKDRIVLSLPREIAPYEVAVFPLLERDELISRAREIYSKVKEKFDAIFDDSGSIGKRYARADEIGIPYAITVDPQTLQDNTVTIRDRDTWNQIRVKEEELINTLEKLFSGEEIIPKGSSNESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H6</accession>
    <name>F4B7H6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps27ae</name>
        <name evidence="8" type="ordered locus">Ahos_0694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775497.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H6"/>
    <dbReference type="STRING" id="933801.Ahos_0694"/>
    <dbReference type="GeneID" id="10600146"/>
    <dbReference type="KEGG" id="aho:Ahos_0694"/>
    <dbReference type="eggNOG" id="arCOG04183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179743_1_0_2"/>
    <dbReference type="OrthoDB" id="25142at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_eS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_eS31_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS31" evidence="7">
        <location>
            <begin position="13"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="63" mass="7062" checksum="62606DC0B0759DD4" modified="2011-06-28" version="1">MAKKEASEAKASVRLYYEISDGKVKLKNKKCPRCGSVMAHHLKPVERWACGKCGYTEFVSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I0</accession>
    <name>F4B7I0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775501.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7I0"/>
    <dbReference type="STRING" id="933801.Ahos_0698"/>
    <dbReference type="GeneID" id="10600150"/>
    <dbReference type="KEGG" id="aho:Ahos_0698"/>
    <dbReference type="eggNOG" id="arCOG01617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_2"/>
    <dbReference type="OrthoDB" id="28487at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd19086">
        <property type="entry name" value="AKR_AKR11C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053135">
        <property type="entry name" value="AKR2_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312">
        <property type="entry name" value="D-THREO-ALDOSE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43312:SF1">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="324" mass="37298" checksum="A7094FA4E50D624E" modified="2011-06-28" version="1">MRERDFGHTGIRVSEIGVGLWSLATDWWAPNVNAEEILKKAYELGITFYDTGDIYGEGKAEELLVKVLGNKRDNIVILTKIGYDFYNKKGRKIEQNFNIDYLEFAIKESLKRLNTDYVDILMLHNPKMNVIRNKEIFDFMQGLKKDGIVRAIGVALGPTLGWGEEGLEAIKMGYEGLEHIYNMIEQYPGKEFLKYRIGNVVRVPHASDALIEGKWPIGENKNLHRSLKNIKWIEEAVNNSKGMLEFAKSKGMKLSQLAIKFVLANPNVSTVVPNITTIKELEEFVKTEELEDLTEEDMSFISSYYEKYYKKLNDESIEETKIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="92" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I9</accession>
    <name>F4B7I9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="16 18">Reverse gyrase</fullName>
            <ecNumber evidence="16">5.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rgy</name>
        <name evidence="23" type="ordered locus">Ahos_0707</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16 18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 1 or 2 zinc ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">Introduction of positive supercoils requires the cooperation of both domains. The helicase-like domain probably does not directly unwind DNA, but more likely acts by driving ATP-dependent conformational changes within the whole enzyme. A beta hairpin in the 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="16">This enzyme is the only unique feature of hyperthermophilic bacteria/archaea known and seems to be essential for adaptation to life at high temperatures. It may play a role in stabilization of DNA at high temperatures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the C-terminal section; belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 16">In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="16"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775510.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7I9"/>
    <dbReference type="STRING" id="933801.Ahos_0707"/>
    <dbReference type="GeneID" id="10600160"/>
    <dbReference type="KEGG" id="aho:Ahos_0707"/>
    <dbReference type="eggNOG" id="arCOG01526">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002886_0_0_2"/>
    <dbReference type="OrthoDB" id="30963at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160097">
        <property type="term" value="F:reverse gyrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17924">
        <property type="entry name" value="DDXDc_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18798">
        <property type="entry name" value="SF2_C_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03361">
        <property type="entry name" value="TOPRIM_TopoIA_RevGyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.510.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01125">
        <property type="entry name" value="Reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034142">
        <property type="entry name" value="TOPRIM_RevGyr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040569">
        <property type="entry name" value="Znf_Rg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01054">
        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505:SF1">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17915">
        <property type="entry name" value="zf_Rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52037">
        <property type="entry name" value="ZF_RG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52036">
        <property type="entry name" value="ZF_RG_N"/>
        <property type="match status" value="1"/>
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    <keyword evidence="13 16 18" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16 18" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="8 16 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RG N-terminal-type" evidence="21">
        <location>
            <begin position="4"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="94"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="19">
        <location>
            <begin position="612"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="22">
        <location>
            <begin position="790"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Topoisomerase I" evidence="16">
        <location>
            <begin position="608"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="16">
        <location>
            <position position="945"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043976"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS52036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="AEE93594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1232" mass="139710" checksum="FF0CD48960214D95" modified="2011-06-28" version="1">MSEELPNLVYMNSCPNCGGEITANRLYKGSACEKCVKEDIQFNSVYDLALYLLKNKNLVNLRSSYEILKELKEVERLFKRVLGSPPLGPQRSWIVRALRGESFAIIAPPGLGKTTFGILMSLYFAEKQRKTLMIFPTKTLVNQVVQRIQEMSKSLEEVPKLAYYYSGITQSQKGELNKILESGDFDIFISTSRYIINNVDYISTKDYKYLFVDDVDSVLKSSKSASAVLKLVGFTEDDIQNVRELLRKSKNSEEVYDEIRKIREKKTGDKIAIFSSATITKGNPVFSALMGFRPGSAVIYLRNVIDSYVQLPSATDEEIIDIVNKIVNKLGSGGLIFVPIDKGVSFANEIASNISGLKAEAISSTSITKLDKFEKGEIDVLVGVATHYGILVRGIDIPWRIRYAIFAGIPRFRFKIGETMHPLAMLKMLTLISLVSKDEEVTKILRIVRNKLRRISPAALVMLANSVKSGKIEDKYIQEAYDLTNKYLRNEEILEKIAEIGEISINNRYISSPDYLTYIQASGRTSRIFGGELTTGLSVLLVDNPRLFELLNRRLSIVLDEVSWNPFDIDNNKIGNNNLHEILKRIDSEREKIRKIKKEGLLTSASVKNIKTVLFIVESPNKAKTISNFFSKPSIRVFNGAIIYETVIGDKVLMVTASGGHVYDLTTKNIGIHGIEVRKNSNLEFIPYYNTIKRCNNGHQFTEYAEGNKCPTCGSTIIKDKIDVINSFRQLALEADEILIGTDPDVEGEKISWDLYLALRPYNPNIKRAEFHEVTRRAIVEAINNSRQFSIPLLQSQVVRRVEDRWIGFSLSTKLQTVFWPQYCSEVLKRTDCGENKNLSAGRVQTPVLGWVINRYNKYNETKRTVFVVKFLNSLSVLVPKQADLDKNDVKVIVYNISKSSETFGPMPPYTTDEMLSDASNLYGISAPEAMRVAQDLFEMGLITYHRTDSTRISNTGIAVAENYLKEILEDKYKEIFKPRTWGEGGAHEAIRPTRPLDENQLRALIEEGELELPKRLTLNHYRIYGLIFSRFISSQIVPLNVDKLKFNIKVLSNGKELKVENPEMDLITNVSLPGGIEVSKLSLYYPRSYFASRDSSYSDTIKKLEECIKSGQCEFTGSIASSFVKSDYQLYTQGELITEMKNKKIGRPSTYATIIATLLKRGYMLESKKVKRLVPSKLGINVYNFLTSKYSKFVSEERTRQLLELMDLIEDGKEDYRQVLKQLYDEIQSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N2</accession>
    <name>F4B7N2_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1906</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7N2"/>
    <dbReference type="STRING" id="933801.Ahos_1906"/>
    <dbReference type="GeneID" id="10601405"/>
    <dbReference type="KEGG" id="aho:Ahos_1906"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14257" checksum="BF063BF55CBA858A" modified="2011-06-28" version="1">MLIQTSSDVLLSIADDLLSRGDVAHASEKYYKAAEEAIKLLTINLGLKDILNIAKESGWDLATLHKAVTEICKKLNNEDIFEYWESAIVLLTVENLSLDVVKDEAENVRKLVKISDEIANRELDKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P3</accession>
    <name>F4B7P3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1917</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776705.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P3"/>
    <dbReference type="STRING" id="933801.Ahos_1917"/>
    <dbReference type="GeneID" id="10601416"/>
    <dbReference type="KEGG" id="aho:Ahos_1917"/>
    <dbReference type="eggNOG" id="arCOG06007">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860786_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="112" mass="12044" checksum="649AA0833D36F21D" modified="2011-06-28" version="1">MAIAYGIVKLFYPPTIFSVIPIVSLIKCPALGIIQLVILGLMVAFAYPVRTRVAGESLLTIRKLAMVTALGYLLFSLMPLAFHVEYIQTYVGLVIAADIINGAFAGVLSSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q4</accession>
    <name>F4B7Q4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rieske ferredoxin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1928</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776716.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Q4"/>
    <dbReference type="STRING" id="933801.Ahos_1928"/>
    <dbReference type="GeneID" id="10601427"/>
    <dbReference type="KEGG" id="aho:Ahos_1928"/>
    <dbReference type="eggNOG" id="arCOG02855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180514_0_0_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="10530" checksum="BE2D1ABC262D2DF8" modified="2011-06-28" version="1">MIYEKEGKEYSFLAVCPHKNRPILSEGYKIDEDKIECPFHHAVFSLITGELMKLPNSKTPCPADCKLIKVEFTSSGVKFYGKPINPELPRKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S1</accession>
    <name>F4B7S1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776733.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S1"/>
    <dbReference type="GeneID" id="10601444"/>
    <dbReference type="KEGG" id="aho:Ahos_1945"/>
    <dbReference type="eggNOG" id="arCOG03722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639988_0_0_2"/>
    <dbReference type="OrthoDB" id="30877at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17730" checksum="0905A69D2EDB2E44" modified="2011-06-28" version="1">MQVEIPDVIYAFITESLGKDASDVLVDAFLSTLDKGNRLKLYVKLSEEYERKEIDGEACWKALSYVFKAIAEIEGMEISSYQDYYSLADYLSFKLNNTEIIKYFLNAEKLHAEYHPRPQDKDSLRVRMDHCKKLIEVGKEYLRKSKSLPEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V0</accession>
    <name>F4B7V0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps7</name>
        <name evidence="11" type="ordered locus">Ahos_0741</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775543.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V0"/>
    <dbReference type="STRING" id="933801.Ahos_0741"/>
    <dbReference type="GeneID" id="10600198"/>
    <dbReference type="KEGG" id="aho:Ahos_0741"/>
    <dbReference type="eggNOG" id="arCOG04254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="OrthoDB" id="45346at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000011">
        <property type="entry name" value="30S ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_uS7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_uS7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="10">
        <location>
            <begin position="29"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="194" mass="22141" checksum="DC515517591A6BA2" modified="2011-06-28" version="1">MSDYELSKLDIRIFGKWDTKVEIRDPSLKKYISLMPVYLPHTGGRHEHRRFGKSRVPIVERLINQLMKPGRNKGKKFLAYNIVRAAFEIIAARTGQNPIQVLVRAIENSAPREEVTRIMYGGIVYYVAVDVSPQRRVDLALRYLVEGARTASFNNPKPIDEALAEEIIAASSNDPKSYAIRKKEETERIALSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z1</accession>
    <name>F4B7Z1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775584.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z1"/>
    <dbReference type="STRING" id="933801.Ahos_0782"/>
    <dbReference type="GeneID" id="10600242"/>
    <dbReference type="KEGG" id="aho:Ahos_0782"/>
    <dbReference type="eggNOG" id="arCOG01117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170329_2_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF6">
        <property type="entry name" value="REGULATORY PROTEIN ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator AsnC/Lrp ligand binding" evidence="2">
        <location>
            <begin position="14"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="90" mass="10132" checksum="94636B79DB82EF47" modified="2011-06-28" version="1">MISNPQSKNVSAIILINTDAGGEEEVYEKLKELKEVSEAYIVYGVYDIVAKIEAQDMDSLRNFVSSTIRKLPKVRSTLTMIIMESSTMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B800</accession>
    <name>F4B800_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ordered locus">Ahos_0791</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B800"/>
    <dbReference type="STRING" id="933801.Ahos_0791"/>
    <dbReference type="KEGG" id="aho:Ahos_0791"/>
    <dbReference type="eggNOG" id="arCOG04180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041630_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041126">
        <property type="entry name" value="FBP_aldo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE/PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341:SF1">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="Sulfolobus fructose-1,6-bisphosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="106"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE93677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="386" mass="42861" checksum="B33A8289467FDC4B" modified="2011-06-28" version="1">MSLMKTTISVIKADIGSLAGHHVVHPDTMAAANKVLAEAKKNGVIIDYYITNVGDDMELIMTHNRGELDTKVHETAWDAFKEATKVAKDLGLYAAGQDLLSDTFSGNLKGMGPGIAEMEIEERPAEPIAIFMADKTEPGAFNYPQYKMFADPFNTAGLVIDPTMHEGYKFEILDVYAGESVTLNAPEEIYDILALIGTPSQYVIRRVYRKADNMIASVTSIERLNLLAGKYVGKDDPVMIVRVQHGFPALGEVLEAFSFPYLVPGWMRGSHYGPLMPVSQRDARATRFDGPPRLIGLGFNVKNGRLVGPSDLFDDPAFDETRRLAEQIADYMRRHGPFMPHRLEPTEMEYTTLPLIQQKLKARFKKEAGVAEAKKSIYEKQVEGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B826</accession>
    <name>F4B826_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B826"/>
    <dbReference type="STRING" id="933801.Ahos_1978"/>
    <dbReference type="KEGG" id="aho:Ahos_1978"/>
    <dbReference type="eggNOG" id="arCOG07723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2393030_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="87" mass="10126" checksum="E466A066170D8392" modified="2011-06-28" version="1">MLKYLVEKVENMESIKIKPETKKKLTEIAGMLEKKTGKRVSYDDVINYLIEKRFKDRKTAEKLFGIAKGVNLYEELLNGRKEDENGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B827</accession>
    <name>F4B827_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776766.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B827"/>
    <dbReference type="STRING" id="933801.Ahos_1979"/>
    <dbReference type="GeneID" id="10601478"/>
    <dbReference type="KEGG" id="aho:Ahos_1979"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135601_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18689">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="8"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="15387" checksum="DBC7F9C9F3CA318C" modified="2011-06-28" version="1">MDVKKMKMVLDSGVILSFLEGEFRDYYEKILNEEIIPYINTVNLTEVYYVLCRKIGKEKAEEIIKSIIKSGYYEIIGVKPIILKYAAECKCAYSISLADCFSIATAKFLKTKALFRREEELAKKSIEEVEFID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B873</accession>
    <name>F4B873_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776812.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B873"/>
    <dbReference type="STRING" id="933801.Ahos_2025"/>
    <dbReference type="GeneID" id="10601524"/>
    <dbReference type="KEGG" id="aho:Ahos_2025"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_9_5_2"/>
    <dbReference type="OrthoDB" id="198277at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041799">
        <property type="entry name" value="Hsp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="126" mass="14359" checksum="0EE73AC7BABD2796" modified="2011-06-28" version="1">MSLTSYVKKEISKKIEELSRSFYENVLPPVDIYEEGGYLNINVDLPGFQKDKIHLRLTSSNEIVIEAERELQEGGVKYLTQRPKRLSRVIRLPVAVKKDSQVTAKYENGVLHISIPVEGTTTIKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A4</accession>
    <name>F4B8A4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF655 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775619.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A4"/>
    <dbReference type="STRING" id="933801.Ahos_0817"/>
    <dbReference type="GeneID" id="10600284"/>
    <dbReference type="KEGG" id="aho:Ahos_0817"/>
    <dbReference type="eggNOG" id="arCOG04130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="OrthoDB" id="7902at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160975">
        <property type="entry name" value="AF1531-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="26055" checksum="BD3C770D4C2E790A" modified="2011-06-28" version="1">MMAENRRPYERKGERRVERREKFAYVLDYLREGNPLDKHKIHKNRPFAQLLGTDYFILMEASTIKELQIEEKVDLEDPSIGVKVDFIISYDDLTSVAKDTLNRVIKKIIEEKEKLFVEFFNRSEPLTLKLHALELLPGIGKKTLREILEERKKKPFESFKDIENRTGIKNIIDIIAERILIEIERKDKYYLFAYPIDHKKPQAAQQQEIIYVGYLEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A7</accession>
    <name>F4B8A7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA methyltransferase, TrmH family, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775622.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A7"/>
    <dbReference type="STRING" id="933801.Ahos_0820"/>
    <dbReference type="GeneID" id="10600287"/>
    <dbReference type="KEGG" id="aho:Ahos_0820"/>
    <dbReference type="eggNOG" id="arCOG01018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_3_0_2"/>
    <dbReference type="OrthoDB" id="372184at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18093">
        <property type="entry name" value="SpoU-like_TrmJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053648">
        <property type="entry name" value="tRNA_Cytidine-2'-O-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041077">
        <property type="entry name" value="tRNAmeth_TrmJ_Sulfolob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786:SF2">
        <property type="entry name" value="TRNA (CYTIDINE_URIDINE-2'-O-)-METHYLTRANSFERASE TRMJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="TRNA/RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="228" mass="26231" checksum="73B41DC94B990D0F" modified="2011-06-28" version="1">MLRVVLVEPEGEYNVGFVARLCKNFEVDELFIVRPKCDINSAIKFSAKGESILRNAKIVDNYDDALKDVEIKIATSSIANNKGDILRKAVFPWELPSVMKSEKIALIFGRESVGLTREEISKADFLLFIPANPNYPTLNLSHAVGIVLYEIWKAKHEIKPKITKESIDLLDKYSKILYDLIKRSEEDYVLYIALKRALIKGINDEEEARTVIKFLRRLYTRILHEDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H6</accession>
    <name>F4B8H6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2057</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H6"/>
    <dbReference type="STRING" id="933801.Ahos_2057"/>
    <dbReference type="KEGG" id="aho:Ahos_2057"/>
    <dbReference type="eggNOG" id="arCOG00335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027059_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="LutB/LldF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153:SF2">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="290"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="383" mass="42480" checksum="5EEC8F407A97BAB0" modified="2011-06-28" version="1">MSWDIAVNRAINNAVPKVYKVLREHPYIEELAKRLREAKLEVLNNLEKYVEETLQSVKSNGGNAYYVHDEKEAQEIVGKIVGKGKIVVMGKSMVAYELGIRKYLESLGNEVWETDLGEFLIQQANEPPSHIIAPAIHMTKESVGKLLKEKIGGVDENSKVEDMVAVVRKFLRDKFVKADVGITGANAIAADTGSVLLVENEGNIRFTTVAPQIHIAIAGVEKILPTLADAMIEPLVQSAYAGLYPPTYVNLTSGPSSTADIEHNRVRPAHGPKEFHLILVDNGRVKANKDNILREALLCIRCGRCHFHCPIYRAIGNIWGDPPYSGPMGAMWSYIVKGDTKPAMYCVHSGNCKEVCPMRINIPRVLEYIKNLGLHSKGKLDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q6</accession>
    <name>F4B8Q6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="2">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="2">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="2">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">uppS</name>
        <name evidence="3" type="ordered locus">Ahos_0890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = tri-trans,hepta-cis-undecaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775692.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q6"/>
    <dbReference type="STRING" id="933801.Ahos_0890"/>
    <dbReference type="GeneID" id="10600361"/>
    <dbReference type="KEGG" id="aho:Ahos_0890"/>
    <dbReference type="eggNOG" id="arCOG01532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_0_4_2"/>
    <dbReference type="OrthoDB" id="8293at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="36"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="212"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="257" mass="30421" checksum="C6D6A256FD0913D1" modified="2011-06-28" version="1">MLQRLLKPVYIIYEKWLWNQIKSGPIPRHVGIIPDGNRRWARENNSSFNEAYLQGYKKLKEVLIWLAELGVKDVTVFALSTENCGKRSSQELSVILKYIKMGIDDLLTEDYIYKYKVKVRAIGQLHKLPEDLRLSISRVVEKSSSFNERSLTLAICYGGRQEILDAVAKLLDEYKKGLIKDIVLNEETFRKYFYDKELEDIDLVIRTSGEMRISNFLLWHLAYSELFFCDAYWPEFRKIDLWRAIRSYQRRKRNFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R8</accession>
    <name>F4B8R8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdenum transport protein ModA-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775704.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R8"/>
    <dbReference type="STRING" id="933801.Ahos_0902"/>
    <dbReference type="GeneID" id="10600374"/>
    <dbReference type="KEGG" id="aho:Ahos_0902"/>
    <dbReference type="eggNOG" id="arCOG00223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125440_2_1_2"/>
    <dbReference type="OrthoDB" id="36889at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051815">
        <property type="entry name" value="Molybdate_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30432:SF1">
        <property type="entry name" value="DNA-BINDING TRANSCRIPTIONAL DUAL REGULATOR MODE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30432">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MODE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="110" mass="12274" checksum="BDF44D445FE1EC98" modified="2011-06-28" version="1">MKFNFKIWIEDDNGNSVLGKGGVELLKEIINTGSISKAAENLNLSYKFAWEYVRKIESEIGGIEMKKGGKNAGGTVVSDKVQQLLKIYEEAMNEVSAVLEKYSKKLNDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S5</accession>
    <name>F4B8S5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S5"/>
    <dbReference type="STRING" id="933801.Ahos_0909"/>
    <dbReference type="KEGG" id="aho:Ahos_0909"/>
    <dbReference type="eggNOG" id="arCOG05923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149110_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="91" mass="10546" checksum="C170AA5FE5173DAE" modified="2011-06-28" version="1">MTEPLVTVYLYKKVEDGKIISAFRIMMYKDSVISIYEDDKLQGGVISDIENGGVDKAYEIIKKYYDDTSDDMIIYGEKDLVDQLLEKFDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T9</accession>
    <name>F4B8T9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">von Willebrand factor type A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775725.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8T9"/>
    <dbReference type="STRING" id="933801.Ahos_0923"/>
    <dbReference type="GeneID" id="10600395"/>
    <dbReference type="KEGG" id="aho:Ahos_0923"/>
    <dbReference type="eggNOG" id="arCOG02900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058577_0_0_2"/>
    <dbReference type="OrthoDB" id="33260at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040929">
        <property type="entry name" value="ArnB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18677">
        <property type="entry name" value="ArnB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="40"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="356" mass="39723" checksum="F84EB1B44C898589" modified="2011-06-28" version="1">MTISLKIKTSHTYVNKNDKIEVGIVIYIVPEKYTSNANIRYIIAVDNSPSMKDHNKFDTAINSAQSLLASIPNTNNVIVILFSNHPKIIYNGQGGNIVYIPKEFGGTTRLHETIRKIIEIANDGVPTKAILLTDGKPKDKTDVRDYEALQIPPNLTFISIGIGKDYNEVILKRISDKSAGVFYHIDDVLQLPNILEEQKTTDTYALNLTLNAPQGFTPFNYDLPIFIPIVDRLVAIYGTMIIDPGSLPVNLTFTANYTDPVDSQSKILPVTITLQRAEDNVVRSTINSNVLAEIKYYRLLKEYADTLAKGKDTTRVMSELMQTAQQTGNQALIEETQKLTGHSKSDLSEVTKKMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U7</accession>
    <name>F4B8U7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775733.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U7"/>
    <dbReference type="STRING" id="933801.Ahos_0931"/>
    <dbReference type="GeneID" id="10600403"/>
    <dbReference type="KEGG" id="aho:Ahos_0931"/>
    <dbReference type="eggNOG" id="arCOG01212">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324042_0_0_2"/>
    <dbReference type="OrthoDB" id="38888at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="211" mass="24082" checksum="24E6721F59E1AF39" modified="2011-06-28" version="1">MEANLVSLCKKCKALGYFGSYVRKEDYVEGVSDINIFAISDDKSLLLELGSYSGISPIVISEEKFREICESGDIICYYILYDSKLICGELPKVEFTINDNTCERLKMSSSSFIKLSFEAFKRNDEIGTLENAYRAIRSLIQYISCSSLRKIPISNSEIKETCIKLNLNFCKEFDNLILLRNMKSPLTPWALNRIYNIINSQIKMDFSSTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X4</accession>
    <name>F4B8X4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0958</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="548" mass="63523" checksum="0C780FB024C1AF66" modified="2011-06-28" version="1">MDFQDLFKLRPVYAFDVNNGKISYVVRDKIPSAYIHDEGKVPLEDVYAEGVEWITDKKLAIIGDPGGSEKREIYIYDRNKVFPLLKDQYDNFGTYFINEDKFLFISNRDGKTLHLYLYDKGDIIQVSKGDNPVENYCLSPSKKRVVYSQGIYDNDLYVYDLDRGEVISKISYPNSEETVSLYCFKDEDNFLFISNKDNYFDIYNFNIEKEEITEVIKSEHEKYEVVPIADKIIYSEDVYGDFKIKIHKGNELVTEGFNSYLTPDQDYIYYIGSTYNRHFDLYRVDILTGKIERLTNSMDNIKGDFVKPKVVKYYSEGGIEINALLYEKGGEGKGVVYIHGGPDWECTDSFNPEIQFLVDRGFKVICPNYRGSTGYGRRFNHLNDKDLGGGDLKDIINSAKLLGVKKVAVTGGSYGGYLTMMAVTKYPDMWCSAAAVVPFVNWFTEKKFEREVLQQYDEMKMGNDENLLRDRSPIFFIDNIKSPLLILAGENDPRCPAEETRQVIEKLKERNIEVEYKIYEDEGHGFSKIENYVDSIRRVVEFIERHCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B902</accession>
    <name>F4B902_ACIHW</name>
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        <submittedName>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B902"/>
    <dbReference type="STRING" id="933801.Ahos_2154"/>
    <dbReference type="GeneID" id="10601657"/>
    <dbReference type="KEGG" id="aho:Ahos_2154"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DGGGPL_red"/>
    </dbReference>
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        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
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        <property type="entry name" value="GGR_cat"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22578">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Digeranylgeranylglycerophospholipid reductase catalytic" evidence="9">
        <location>
            <begin position="180"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE95025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="452" mass="50587" checksum="A0317C57880F9D6B" modified="2011-06-28" version="1">MKNNYDVLIIGGGFAGATTAWHLANHGLKVLLIDSKPWNRIGDKPCGDAVSKEHFDNLGMPYPEGEQLEQKIEGIKLYSPDMKTEWIVKGEGFEINAPAYTQRLLKEASNRGVEVLDMTTAMKPIFEDGFVKGAILFDRRHNQQIEVRAKVVVEATGYSMSFRSKLPQGLPVTENLDDKDADIAYREVAYTKDDIDEPQYLKIFVNQKASPGGYWWYFPKGKNKVNIGLGIQGGMGYPSIYTFYDKYLNEFGSDVDRNRLIVKGGALVPTRRPISTLAWNGILVVGDSAFTANPVHGGGKGSAMISGYCAAKAILNAFEVGDFSAKTLWSANLCYIERYGAKQASLELFRRFLQKLSDDDINYGMRKKVIKEEDLLEASAKGDLQLSVAEKAMRIIAGLGRPSLLLKLKVVAEYMKKAKDLYREYPSDPNNLEQWKSEVNKLLLDFENLMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B921</accession>
    <name>F4B921_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">KH type 1 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776959.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B921"/>
    <dbReference type="STRING" id="933801.Ahos_2173"/>
    <dbReference type="GeneID" id="10601676"/>
    <dbReference type="KEGG" id="aho:Ahos_2173"/>
    <dbReference type="eggNOG" id="arCOG04150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064992_3_1_2"/>
    <dbReference type="OrthoDB" id="7870at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000076">
        <property type="entry name" value="KH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010326">
        <property type="entry name" value="PRK13763.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="74"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="177" mass="19879" checksum="A5DAC46789780E94" modified="2011-06-28" version="1">MFISVPDEKINIVKSLISKLKELSGAEITYDENTKTFNVDSSQNPYDAMKVISVIRAIGLGFSVDESLKLLSDDYMLDVIDLKQSVGSNPETLRRIKGRIIGENGKAKKIIQEYTGVSISITDHFVAIIGIYDQVQIARKAIELLIEGKEHSTVYKYLDKAEAELVYLSKNRSYNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B959</accession>
    <name>F4B959_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775768.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B959"/>
    <dbReference type="STRING" id="933801.Ahos_0966"/>
    <dbReference type="GeneID" id="10600438"/>
    <dbReference type="KEGG" id="aho:Ahos_0966"/>
    <dbReference type="eggNOG" id="arCOG05928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369522_0_0_2"/>
    <dbReference type="OrthoDB" id="34291at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="204" mass="23761" checksum="E5B4B3EB522D07B2" modified="2011-06-28" version="1">MLFEISIDKLSQEEKGVRLISNSGHLVSFSSSLFNELSRLGIDKRTFAEIVIDFLNEGTKYYSTYIKPISNVEECKYYSRIFEFWITSSLTSKQMFAVITNYDEISEVLIIDPKVFNYAAEKLLNYASTKDCMKFSMPFIYKFVVFETFNIFKKKFNANSEKIIGKNNEKFLIAKNVEDNALIWKIEAPQFSYVSNYEENKASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B971</accession>
    <name>F4B971_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B971"/>
    <dbReference type="STRING" id="933801.Ahos_0978"/>
    <dbReference type="KEGG" id="aho:Ahos_0978"/>
    <dbReference type="eggNOG" id="arCOG08344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519558_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9390" checksum="9896387CFA00159B" modified="2011-06-28" version="1">MSSAEDPDVDILNKNGLVNVYVDLRGKEINTKRLLVRADNSHVYIYDPDSNSIVKIIYVNELIDPSTLSVSEKNGIINISLKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B973</accession>
    <name>F4B973_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GtrA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0980</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775782.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B973"/>
    <dbReference type="STRING" id="933801.Ahos_0980"/>
    <dbReference type="GeneID" id="10600452"/>
    <dbReference type="KEGG" id="aho:Ahos_0980"/>
    <dbReference type="eggNOG" id="arCOG02228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880932_0_0_2"/>
    <dbReference type="OrthoDB" id="44002at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051401">
        <property type="entry name" value="GtrA_CellWall_Glycosyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA_DPMS_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38459:SF1">
        <property type="entry name" value="PROPHAGE BACTOPRENOL-LINKED GLUCOSE TRANSLOCASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA_DPMS_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA/DPMS transmembrane" evidence="7">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="135" mass="15238" checksum="65DA94734F5C7961" modified="2011-06-28" version="1">MIVGGLGTLVNEGVFILTARDMPIFFSLGLAIELSIIFNFILNDIWTFKDRRVGSFIKRLLKFHVSSFSGGIVQYITVILLLVAFLHFSNVSEILLFLFFSYIKAQSLFLAVINFIGIVSGFAVRFITSLKYVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A3</accession>
    <name>F4B9A3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775812.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A3"/>
    <dbReference type="STRING" id="933801.Ahos_1010"/>
    <dbReference type="GeneID" id="10600483"/>
    <dbReference type="KEGG" id="aho:Ahos_1010"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_5_2"/>
    <dbReference type="OrthoDB" id="29061at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052952">
        <property type="entry name" value="MFS-Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23527">
        <property type="entry name" value="BLL3282 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23527:SF1">
        <property type="entry name" value="BLL3282 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="8"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="378" mass="42259" checksum="4EA865B059395D6A" modified="2011-06-28" version="1">MKSKELESVLIIFITSLSFFLSYFSRIAWSIVSVYSSLKPTELQDSIIFSLFFIGYVIVQIPSGFIANSYPKVTSITSLLGLSFASFLSGYANSIAFEYFSSLLMGLFAGWIYPTTIRILSCKFSGKELPIAIGYYSLAWPLSIVLAGVTLPYLSINFGWRFPYYLISIISLFTALSYLPMPIKNSKIERKSLFLVAKNRNVLIVSFSGFLFFLSYWIITLYAYKYFLTLGMNSYIAGLTYSMLALAGIPSTVIAGYIIRKIGIKNSLAFFEGIYGLLTFNFSYYLSMIQIFIIASIMGFVRFIITPANSSAVSVIDIKNAGSVSGFANFFWQSSGILAPLIASIIITSLGFKLLWLFSGFIIILSSIIYLFLLKIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B4</accession>
    <name>F4B9B4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775823.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9B4"/>
    <dbReference type="STRING" id="933801.Ahos_1021"/>
    <dbReference type="GeneID" id="10600496"/>
    <dbReference type="KEGG" id="aho:Ahos_1021"/>
    <dbReference type="HOGENOM" id="CLU_965051_0_0_2"/>
    <dbReference type="OrthoDB" id="374114at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="288" mass="32490" checksum="A80685A88DF4F75D" modified="2011-06-28" version="1">MSKIVVGLATILVLISSAMLPSIIMANSSSSTTYFMDYSINYHSYHSGKYFTTSCKESLINLNITSTKIGNNKYNNTIIVTGYIFTNYSSPFMTSVSNKTIHHVFNFTSKYPLAKEITIFNLSSLLNMTLNITNEFKIFNITVNYKINYQPNGTESVVFNGKQYTLDSYKAFLYLSALGKNKYANITYTSTMIGNILTFQKGILYKLYLTGESQKNITFYNFLNTYSKGNETLCILLKNTNIKLDSVMMPNQENVNSTQQESIKPEEFLIPTGIIGLLAAALILVRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G2</accession>
    <name>F4B9G2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase IV</fullName>
            <shortName evidence="15">Pol IV</shortName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">dbh</name>
        <name evidence="17" type="ordered locus">Ahos_2237</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777023.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G2"/>
    <dbReference type="STRING" id="933801.Ahos_2237"/>
    <dbReference type="GeneID" id="10601742"/>
    <dbReference type="KEGG" id="aho:Ahos_2237"/>
    <dbReference type="eggNOG" id="arCOG04582">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012348_1_2_2"/>
    <dbReference type="OrthoDB" id="372207at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000009">
        <property type="entry name" value="DNA polymerase IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01113">
        <property type="entry name" value="DNApol_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050116">
        <property type="entry name" value="DNA_polymerase-Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002292">
        <property type="entry name" value="PRK01216.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076:SF33">
        <property type="entry name" value="DNA POLYMERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076">
        <property type="entry name" value="DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="12 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 15 17" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="10 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 15" id="KW-0515">Mutator protein</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="16">
        <location>
            <begin position="3"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Substrate discrimination" evidence="15">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE95108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="354" mass="40240" checksum="33E72F51DDB5A37C" modified="2011-06-28" version="1">MIVLFVDFDYFFAQVEEVLNPELKGKPVAVCVFSGRFKDSGAIATSNYEARKLGIKSGMPIPKAKEIAPNAIYLPIRKDLYKQVSDRIMYGILSKYSSKIEIASIDEAYLDITDKVKDYYEAYQLGKKIKDEIYQKEKITVTIGIAPNKVFAKIIAEMNKPNGLGILKPEEVEGFIRSLPIDEVPGVGDSIFSKLREMGIKYLYDVLKVDFEKLKNEIGKSKASYLYSLANNTYAEPVKEKVRKHIGRYVTMKKNSRDIKEILPYLKKAIDEAYSKANGGIPKTLAVVAIMEDLDIVSREKTFNFGISKDKAYLESEKLLEEIIKSDKRRLRRVGVRLGKIYKSTTLDNFFNNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I4</accession>
    <name>F4B9I4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777045.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I4"/>
    <dbReference type="STRING" id="933801.Ahos_2259"/>
    <dbReference type="GeneID" id="10601765"/>
    <dbReference type="KEGG" id="aho:Ahos_2259"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="204"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="525" mass="59325" checksum="ED251525A131BA7F" modified="2011-06-28" version="1">MPYQIILSSDRGSFTDYGGSSVLGYVACMPSRLVPRLFMDRFFTPRVPLKGDNAAFAPYALRKVEAILSNSGFDVTVVPPEKIEKYVKDAKILGITVHDPFGLNPVSAKLSFIFGGGPTWTAQFFSEFGEKVSKLKEKYNFKVIAGGPGSWELSLRKPDWVDVVFLGEAEVDLPIIAKKIIDGEDVPPIVKGRNAKIEEIPTIKNPARLGEVQITRGCPRGCKFCSVTPETFRSIPLDQIKKEVEVNLKGGWRRVEFITDDVMLYGSSKLRTNHDAIVKLFTEVMNMGVDGIWFPHISAPAVRESPKTVKAIAEIARYDVDRAAAPVVGLETGSLKILEKYMTAKAFPWTPREWKDVILDATAIMNDNYIYPCYTMTIGYPEEDERDVEDSINLVQSIIDHKFTAWIFPLPVIPISTTYISKNPYPKPETLPQNYWDLLYISWHYDLKVTRDLIPILTGGIKNKLVRKFVQIMIDKIFSNIDYIFLQLKNTKGEFSKTFSQINLNNTLGVIKSIYWLIRITAKPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-03-15" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I5</accession>
    <name>DN7B_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-binding protein 7b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">7 kDa DNA-binding protein b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Aho7b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="37274" last="37274">
            <title>The archaeal '7 kDa DNA-binding' proteins: extended characterization of an old gifted family.</title>
            <authorList>
                <person name="Kalichuk V."/>
                <person name="Behar G."/>
                <person name="Renodon-Corniere A."/>
                <person name="Danovski G."/>
                <person name="Obal G."/>
                <person name="Barbet J."/>
                <person name="Mouratou B."/>
                <person name="Pecorari F."/>
            </authorList>
            <dbReference type="PubMed" id="27853299"/>
            <dbReference type="DOI" id="10.1038/srep37274"/>
        </citation>
        <scope>DNA-BINDING</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Highly stable from pH 0 to pH 12.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Hyperthermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 7 kDa DNA-binding/endoribonuclease P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777046.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I5"/>
    <dbReference type="SMR" id="F4B9I5"/>
    <dbReference type="STRING" id="933801.Ahos_2260"/>
    <dbReference type="GeneID" id="10601766"/>
    <dbReference type="KEGG" id="aho:Ahos_2260"/>
    <dbReference type="eggNOG" id="arCOG05888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929990_0_0_2"/>
    <dbReference type="OrthoDB" id="33867at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003212">
        <property type="entry name" value="DNA-bd_7a-e_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045555">
        <property type="entry name" value="Sul7d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02294">
        <property type="entry name" value="7kD_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000439046" description="DNA-binding protein 7b">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P61990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="27853299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="27853299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="62" mass="7121" checksum="B6C0C14592700B3E" modified="2011-06-28" version="1">MATKVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDDNGKTGRGAVSEKDAPKELLDKLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J4</accession>
    <name>F4B9J4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777055.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J4"/>
    <dbReference type="STRING" id="933801.Ahos_2269"/>
    <dbReference type="GeneID" id="10601775"/>
    <dbReference type="KEGG" id="aho:Ahos_2269"/>
    <dbReference type="HOGENOM" id="CLU_1021570_0_0_2"/>
    <dbReference type="OrthoDB" id="40355at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="272" mass="31869" checksum="08CD6840847F2BBB" modified="2011-06-28" version="1">MLVCRQGLKDNDVTLYDYGSYQVIENGKVRYFYSGEIILKVSDISSNVLDKLIYAINKGIRYFFFEGYLLQYIPSFGYGNYFIFKTEIKDEELNNKSLQLLEGKVSEDVYIDYLMKYQGVKGETIGVIDEFYTLTNELRLPKYEPMELTQCKELEVKFEDKYVEIFNVRFRILDISYFDFLSKYISVLKIIKGNYKGEIKTSLGEGIIYHKIGKIKNLTFSFTKICGKYRLDTPENCIIGDGISFHTKNKDEIDQLMYCLENLKTLRDSLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J6</accession>
    <name>F4B9J6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-amylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2271</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777057.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J6"/>
    <dbReference type="STRING" id="933801.Ahos_2271"/>
    <dbReference type="GeneID" id="10601777"/>
    <dbReference type="KEGG" id="aho:Ahos_2271"/>
    <dbReference type="eggNOG" id="arCOG03278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033691_0_0_2"/>
    <dbReference type="OrthoDB" id="64936at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10795">
        <property type="entry name" value="GH57N_MJA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052046">
        <property type="entry name" value="GH57_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306:SF1">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="3">
        <location>
            <begin position="6"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="446" mass="52896" checksum="4D5BE83EE5CB8573" modified="2011-06-28" version="1">MVDKIILGFEVHQPFRIRKDAFWNPRFRGNILERFFDMELNKEIFDRVKRKCYIPATKIILEEIENAEDEGREVKFFFSLSGTFIEQAEKWGKDVLELFQVLSSTKKVEFLGQTYYHSISSLWEDKTEWKEQVREQKEIIKEYFKQEPQVFENTELLTNPQILDMVQEIGYKGIIMEGKESTLNGKNPNFVYRRKGLTILFRNYRLSDDIAFRFSSRNWDQYPLTADKFANWVKDSPGQVVTIFVDYETFGEHHWPESGILDFLRWLPRELNKRGVKFALPRELINESYYDIDIQGTSSWADINKDESSWLGNIMQWAYDEAVRRAEMPSKELGGDYLKAWKYFTTSDNYYYLFTGGGGPAEVHNYFNAYSTPVDAFINEFYAINAFLNEELHKLGINNEPFFFIKNGKRSSVAWNKKEFEEIIKRDGSLKDHLKYLKEWLGNEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N6</accession>
    <name>F4B9N6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cobD</name>
        <name evidence="12" type="ordered locus">Ahos_1068</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N6"/>
    <dbReference type="STRING" id="933801.Ahos_1068"/>
    <dbReference type="KEGG" id="aho:Ahos_1068"/>
    <dbReference type="eggNOG" id="arCOG04274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00380">
        <property type="entry name" value="cobal_cbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002281">
        <property type="entry name" value="PRK01209.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308:SF1">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="34545" checksum="B0E5DBBA5B78574F" modified="2011-06-28" version="1">MLLPILFLALAIDLLFGEPPIYVHPVVYTGKISEKLIKPYKGYSYGILIWLISVIPVEIAFSILPLYIPIIPIKLILLALFLKTTFSIKMLYNIVSKSRELDERARNLVQQIVRRDLSNADKGHIASAAIESLFESLVDGIISPLFWFLILGLPGAMLQRLANTMDSMVGYKTKELYKEGYFSAKVDTIMNYIPARITGLLMVIAGKLLGIKQSSNPFRFLKNTQIESINARYPICIASALLGVKLEKKGYYTVGEGKLPGKEEIEKSLKLFKLTLFLFLILLSIIYYGLYGLAFFSYPYGLVEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P4</accession>
    <name>F4B9P4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fructose-bisphosphate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775876.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P4"/>
    <dbReference type="STRING" id="933801.Ahos_1076"/>
    <dbReference type="GeneID" id="10600550"/>
    <dbReference type="KEGG" id="aho:Ahos_1076"/>
    <dbReference type="eggNOG" id="arCOG04044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_0_2"/>
    <dbReference type="OrthoDB" id="6329at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="2">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR038992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="270" mass="29744" checksum="D111B8E6541ADB4B" modified="2011-06-28" version="1">MSGSTIRLKRLFEKGKAFVVALDHGLVMGPLKGIENPVEIVSKLSNIPDALQMTPAMLKLVEENFYTRNSPMLIARLDTANVWRKEKKYSQGYYSMVYQVKEAVEAGADAVVTYLVVGYGDDRVEGYNIEALAKARAEAHDYGLPFIIEPLYVSPDNPDSIKDVNLVKYVTRLASEIGADILKVDYTGNKEEFKEVVNVAFAPILIRGGPKTKDDKEFLLMLKDAVDAGASGVTVGRNLWQSKNPTAMAKAIADVIRGKSVEEALKIWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P7</accession>
    <name>F4B9P7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase BadF/BadG/BcrA/BcrD type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775879.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P7"/>
    <dbReference type="STRING" id="933801.Ahos_1079"/>
    <dbReference type="GeneID" id="10600553"/>
    <dbReference type="KEGG" id="aho:Ahos_1079"/>
    <dbReference type="eggNOG" id="arCOG03659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_0_2"/>
    <dbReference type="OrthoDB" id="39947at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052519">
        <property type="entry name" value="Euk-type_GlcNAc_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43190:SF3">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="302" mass="32635" checksum="F12933BC32BBC5A6" modified="2011-06-28" version="1">MILVGVDAGGTSTISIAYTCEGKFIGIGESGPGNFHNVGLENAVSNIRDAIYKSTKGLKPDVACIGLAGLDSKYDYEVLSSALKDLAKDTIIEHDSFISLYAETRGKPGIIIISGTGSVVMGYDGKRRLRVGGAGWLLSDEGSAYWIGRKALRLLVKMIDGREEKTLLADLILQKLSASGLDDLIKWAYHEGHKIEEIASLAQIVDIAANKGDKKAQELFYLAAKEISNDVIYISKKLGIKTAYMSGGMFSSKLFKSYVVDLLKKEGIIALDKKKSPEFGALLIAFDKAGCINEPEEPHLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q8</accession>
    <name>F4B9Q8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="6">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775890.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q8"/>
    <dbReference type="STRING" id="933801.Ahos_1090"/>
    <dbReference type="GeneID" id="10600564"/>
    <dbReference type="KEGG" id="aho:Ahos_1090"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_2_1_2"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 6" id="KW-0533">Nickel</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="7">
        <location>
            <begin position="53"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="14851" checksum="60427FBD09265104" modified="2011-06-28" version="1">MNVEKISVSIPKDIMEQLDKFMKTSSSLDRSKIFQIAIKNFLDENSLEDVDVVGIINIIYDDEVTDEITKLQHEKLSSIISTLHIHLNEKECMEAIAVKGKKSDLVKLTSSLSQIKGVKKAKLLTYEVKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S0</accession>
    <name>F4B9S0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cupin 2 conserved barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775902.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S0"/>
    <dbReference type="STRING" id="933801.Ahos_1102"/>
    <dbReference type="GeneID" id="10600576"/>
    <dbReference type="KEGG" id="aho:Ahos_1102"/>
    <dbReference type="eggNOG" id="arCOG03000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507456_0_0_2"/>
    <dbReference type="OrthoDB" id="33544at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06992">
        <property type="entry name" value="cupin_GDO-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047183">
        <property type="entry name" value="GDO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517">
        <property type="entry name" value="1,2-DIOXYGENASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41517:SF1">
        <property type="entry name" value="CUPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="3">
        <location>
            <begin position="75"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="181" mass="20605" checksum="696A34BAB86A8C64" modified="2011-06-28" version="1">MEHVLEREDRQEELTKEIKSIVKEIENDDLKVVTFMEYTSPPKEIKPKVIKFNKALELLRLIAKQGKIQKGVAKIMFYSPSTERSRGLTPTMMAGFQYIEPGVATKPHSHNMASIYLVVKGKGYSIIDGKKYYWEEGDIFVVPANAVHSHVNTSNEEVVLFDVTDSGLLENLGILEFKEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA38</accession>
    <name>F4BA38_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histidinol-phosphate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1151</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775951.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA38"/>
    <dbReference type="STRING" id="933801.Ahos_1151"/>
    <dbReference type="GeneID" id="10600625"/>
    <dbReference type="KEGG" id="aho:Ahos_1151"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="41"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="371" mass="42334" checksum="1B25A0B5FA84801E" modified="2011-06-28" version="1">MYKKFLNLFPPFYQGFRIAPTEIPKWLKEAKEYDYSDIKEGIRLHLNESPYSPPNFIIQEVEKYLSQGNRYQHPDLTSRFKELAAEYNKVEPSNIFPTPGGDGALRAIFYNFLNPGDAVTYNFPSYSMYSVYSTVRGLKVNKVNLVENGDWWKEDIDKLLDFAKKSKLVVIDDPNNPTGSPMLKANKELIFTLAESINGFLVLDEAYYEFSGYTAASLVNEYPNVMIVRTLSKAFSLASYRIGYLIANKEVVDALTKTSTPFDVALPSLIAGITALENPSYARKIVNEITENREYLYSSLKKLGLKVYKSVTNFLFVKDNRDLLNPLMERKIAIRKPIDGFYRITVGTREQCEILVKALGEILESGNSKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA59</accession>
    <name>F4BA59_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">H+transporting two-sector ATPase C subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA59"/>
    <dbReference type="STRING" id="933801.Ahos_1173"/>
    <dbReference type="KEGG" id="aho:Ahos_1173"/>
    <dbReference type="eggNOG" id="arCOG02455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18120">
        <property type="entry name" value="ATP-synt_Vo_Ao_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004888">
        <property type="entry name" value="PRK06251.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="9">
        <location>
            <begin position="40"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="10481" checksum="A5EF6D565F2AEE2E" modified="2011-06-28" version="1">MKRMKSAILLLSLFLGMITAIPVGAQVGYSSPQGFEGVNIGAGLAIGLAAIGAGVAVGMAAAAGIGVLTERRDMFGTVLIFVAIGEGIVVYGLVFAVLMLFAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA71</accession>
    <name>F4BA71_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2286 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775984.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA71"/>
    <dbReference type="STRING" id="933801.Ahos_1185"/>
    <dbReference type="GeneID" id="10600659"/>
    <dbReference type="KEGG" id="aho:Ahos_1185"/>
    <dbReference type="eggNOG" id="arCOG04251">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126372_0_0_2"/>
    <dbReference type="OrthoDB" id="15027at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017006">
        <property type="entry name" value="UCP032756"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10051">
        <property type="entry name" value="DUF2286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032756">
        <property type="entry name" value="UCP032756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16434" checksum="7FF66E79F69BB8D1" modified="2011-06-28" version="1">MKVIILKSENGKITSEKITEGDLAEVVRNTAIEALKEWNELTSDFIIMKDSQEAKLPLPLKPDVYEAVKNFLAGKEKSAAILKIPIFIISYDNIWQEENFQDKRVYVVSYYLNDDLKKELIEYAQGVTSEEKPQDSGEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T2</accession>
    <name>F4B3T2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776035.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T2"/>
    <dbReference type="STRING" id="933801.Ahos_1236"/>
    <dbReference type="GeneID" id="10600713"/>
    <dbReference type="KEGG" id="aho:Ahos_1236"/>
    <dbReference type="eggNOG" id="arCOG00095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424917_0_0_2"/>
    <dbReference type="OrthoDB" id="2513at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00981">
        <property type="entry name" value="arch_gltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01907">
        <property type="entry name" value="GlxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035710">
        <property type="entry name" value="Archaeal_gltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012075">
        <property type="entry name" value="Glu_synth_lsu_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673">
        <property type="entry name" value="TUNGSTEN FORMYLMETHANOFURAN DEHYDROGENASE, SUBUNIT C (FWDC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673:SF5">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036632">
        <property type="entry name" value="GOGAT_lg_1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="656" mass="73621" checksum="13EB660BF4531139" modified="2011-06-28" version="1">MISPSGCGVFGILRKRNAPKIKGKDVVKAIDLIRYRGSDKGAGFAVFNLKEGNSYYIKAFYFGDGDEIKREIEDKGIRIKGFNEKYFGELCDCDFEISLGSIASLKKVVRNINEILWNNGKKGRIYSAGKSLQVYKGVGYPADIARQYNIYEVEGDMWIAHTRQPTNSPGSYPYWSHPFSAFDVAIVHNGDVSSFGANVEFLQSRGWGGFVGTDSEVMAFLFEELISEGLSVEEVTRIMSNPSRRFSKLNVEEDYIYRNARLDGPFTAIIGYDSGNDLFMIGIADRAKFRPVIIGEDENYYYIASEENQIRLLSPSAKVWTLEPGNYFIASLLKGLINPGREVSKISSFSKPIFYTEKFDIDASGLGYKDVNKAIMEFVNKSGKKEVTVINLLGHRYLGISFPKAGLRLNLYGVVGNAMANLNENNVFHVYGNVADDCCDTMQGGKVVIHGDARDVLAQTFQGGYIYVKGNAGNRVGIQMREYMNKRPYLVIGGMVDDYLGEYMAGGVIMVLGLGIKGEPVGNYIGSGMVGGRIYIRGKVNPEKIGLQPSRQEMVRFLKALLLDNMISEEEYNKLKEMPYIEVIKELNGEAKKYAQKLFEEKVGIPTYEYRELTEEEIKELEPVARDYAIETNQNKDTMVEMLKEKYTVITSSLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U6</accession>
    <name>F4B3U6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl44e</name>
        <name evidence="5" type="ordered locus">Ahos_1250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776049.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U6"/>
    <dbReference type="STRING" id="933801.Ahos_1250"/>
    <dbReference type="GeneID" id="10600729"/>
    <dbReference type="KEGG" id="aho:Ahos_1250"/>
    <dbReference type="eggNOG" id="arCOG04109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114645_2_1_2"/>
    <dbReference type="OrthoDB" id="52456at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004425">
        <property type="entry name" value="PRK05767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="10932" checksum="6992AFAA8234ABD4" modified="2011-06-28" version="1">MKVPKTITTYCPKCKTHTPHSVSLYKGGKRRTLAEGQRRYDRKNLGYGGQRKPEQKRFAKVTKKAVLMLKCTKCGYVIMKPGMRIKKMEIVEVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X4</accession>
    <name>F4B3X4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, PadR-like family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X4"/>
    <dbReference type="GeneID" id="10599483"/>
    <dbReference type="KEGG" id="aho:Ahos_0043"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF2">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="149" mass="17630" checksum="599421FDDCC9AED9" modified="2011-06-28" version="1">MKMNLERLRKGTLKLLILEALNDKPMHAYEIIKSIEKKFHGIYKPSPGSLYPVLKQLIESGMITVEEKDDKKIYIITDKGKEAFNKMKTEMKNVFTKNNQYRKLVNQLFEIGLIIYNFRDKLSEEDYEKINNIINECKNEIEDLLNKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z4</accession>
    <name>F4B3Z4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774875.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z4"/>
    <dbReference type="GeneID" id="10599504"/>
    <dbReference type="KEGG" id="aho:Ahos_0063"/>
    <dbReference type="eggNOG" id="arCOG08486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2581174_0_0_2"/>
    <dbReference type="OrthoDB" id="36069at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="80" mass="9056" checksum="74E3B14BA733CE27" modified="2011-06-28" version="1">MTEIITNIGDLREFLEKQESLGVKNLEIVISADVLEKSPEIANKYGYSIIDGEDLPSGYFKLTLEYRGIGKSNSHKQFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B406</accession>
    <name>F4B406_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774887.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B406"/>
    <dbReference type="STRING" id="933801.Ahos_0077"/>
    <dbReference type="GeneID" id="10599517"/>
    <dbReference type="KEGG" id="aho:Ahos_0077"/>
    <dbReference type="eggNOG" id="arCOG04583">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107501_0_0_2"/>
    <dbReference type="OrthoDB" id="18536at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="37"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="22993" checksum="829AA747C040FF55" modified="2011-06-28" version="1">MEMKEDEIKLMNMNAYEYIVHRRKPIEIKMNESPVGDIGCGSGQNCKLLKGFVICLDIAEKQLKEAKKRGCENLIQADMEFLPFRDSSFKLLMYIASIHQLPSPNNALSEATRVLKKCGKIIITVWLRQIRFLFRKEVIVKSNINGLVVMRYYRLYWPWELKKICEKKGFVTLDYKVYRVRSILPNNAIYIGYKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B409</accession>
    <name>F4B409_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.311</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA uridine(34) acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Ahos_0080</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="15"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B409"/>
    <dbReference type="STRING" id="933801.Ahos_0080"/>
    <dbReference type="KEGG" id="aho:Ahos_0080"/>
    <dbReference type="eggNOG" id="arCOG01361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="7"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEE92973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="469" mass="54195" checksum="EAD164F549FEB3B2" modified="2011-06-28" version="1">MLKKPVRTISGVAPVSIMTHPYRCPHGKCIFCPGGVEYNTPQSYYGREPTLMRAIENNFDPYCQVRSRLEQYFENGHFPNKVELIIMGGTFLSLPLDYQDWFVTNAIEAMNNFPKKKEKDKFVYLEEAQLRNETAQIRCVGMTIETKPDWGKEWHANQMLRLGATKVELGVQTIYDDILKKVNRGHTVKDSIESTRILKDSGFKIVYHIMLGLPGADPDKDLETIKEIFTNSDFMPDMLKIYPTLVVETAPLAELWKRGLYTPYDTDTLVEIISEAYRYIPKWVRIMRIQRDIPANIILAGNKKGNIRELVEKRAEEKGIEIKEIRYREVGVAWLHRKIKPDRISLHKEIYEASGGTEVFLSFESNNGLIIGYLRLRKPGNSHRKEIDEKTTIVRELHVYGEEVPIGEKKDEAYQHKGYGSALLKEAERISLEEFDAKKILVLSGIGVREYYRQKGYIRYGPYMAKQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B418</accession>
    <name>F4B418_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="6">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_0089</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774899.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B418"/>
    <dbReference type="STRING" id="933801.Ahos_0089"/>
    <dbReference type="GeneID" id="10599529"/>
    <dbReference type="KEGG" id="aho:Ahos_0089"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="OrthoDB" id="30755at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000049">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053399">
        <property type="entry name" value="2-oxoacid:Fd_oxidored_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041171">
        <property type="entry name" value="Oxoac_fdxbeta_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF2">
        <property type="entry name" value="PYRUVATE FERREDOXIN_FLAVODOXIN OXIDOREDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="304" mass="33895" checksum="BCF417227F96EE0A" modified="2011-06-28" version="1">MQEHKPVFVDWCPGCGNFGILRAEEMAIRELGIDPKKVIVVSGIGCSGKIPHFIQLPIGGVHTLHGRSIAYATGIKLSNPSLEVIVNIGDGDGLGIGMGHFVHAGRRNIDMTVIVHDNGVYGLTKGQASPTLHRGLKTKSLPRPNINDAVNPISVALSAGYTFVARGYAYDVMHLKDLIVKAIKHKGLAFIDVLQPCPTYNDINTKEWYDKRIYKLDNDPTWDPKVKSEADQQTKFERAMLKALEWGDKIPIGVFYENELVPTFEERIMQNIPNYLDYYPAKQEIEKNGESTTKIDELIKAKRV</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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        <source>
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</entry>
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
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        </source>
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    </evidence>
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    </evidence>
    <sequence length="107" mass="11997" checksum="EA850786E55A8BAF" modified="2011-06-28" version="1">MKVSIYPEKDSLEMCFEGSTIKIFLIGNEVHIAEEVTYEVTTGEVLSKIQIVIKDGKAYLQSPFGLNEISAPENIFKGIKAVLEEIKEKHKVLYDKFNSLIPTSTTS</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <dbReference type="PubMed" id="21607549"/>
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            <strain evidence="3 4">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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        <location>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="172" mass="19319" checksum="2DBB2806866B2F69" modified="2011-06-28" version="1">MVMEMERVTIIGIIFAILVVGWILATGQWAYGNVVGPLVNHSKIPQLKITYVNAYENAKGTYLILNITDCDGPDAYPASAPMMEIYNSTWHVFLYSYNISNDTVKVIQAPWNENKKDYTNWYSGFVVILGSEAQFQLQLPFHLSPGTYHIKLYTPAVSSKVLAKQTATFTIS</sequence>
</entry>
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    <accession>F4B447</accession>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774928.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B447"/>
    <dbReference type="STRING" id="933801.Ahos_0118"/>
    <dbReference type="GeneID" id="10599558"/>
    <dbReference type="KEGG" id="aho:Ahos_0118"/>
    <dbReference type="eggNOG" id="arCOG03016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_870449_0_0_2"/>
    <dbReference type="OrthoDB" id="213145at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051783">
        <property type="entry name" value="NAD(P)-dependent_oxidoreduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079:SF6">
        <property type="entry name" value="NAD(P)-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48079">
        <property type="entry name" value="PROTEIN YEEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="319" mass="36500" checksum="0A80E0816866C836" modified="2011-06-28" version="1">MKYLLLGLGFVSTHVAEYLSKFGEVKVTYKSLNPVKRIYAEDILKGKADVIKLDPLQDKEKLKEEISSSDVIVNFIGEIQGSEESLRVANVEIPKLIAQTVGETYNEGKKKILIHFSGLLGKTGNNVKPEEPHLQGINPSTPFEKTKYEGEKEVYQISQKYDFPLVILRPTLVYGRYSAHIQFITMYNFAKKGIIPRLSFQFNTISAYYIAEMIKNLSDFKGRKYFYATECSPVNVTRFFELMAKGLGINKQIHLYVPEFLAKLVLPSYIKSLLKYTKSTYDCTESKNLVNNLTFDENEVINNAIFLRRLEKEKSLIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B461</accession>
    <name>F4B461_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-binding protein Ahos_1286</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1286</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776085.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B461"/>
    <dbReference type="STRING" id="933801.Ahos_1286"/>
    <dbReference type="GeneID" id="10600767"/>
    <dbReference type="KEGG" id="aho:Ahos_1286"/>
    <dbReference type="eggNOG" id="arCOG04179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="OrthoDB" id="7912at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.140:FF:000004">
        <property type="entry name" value="DNA-binding protein PAE3044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003268">
        <property type="entry name" value="PRK04239.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="13807" checksum="C10067F494624632" modified="2011-06-28" version="1">MSEDEYDPELQNLLARRAQEESRRALEERRKREEYEKQKEALLRVILTPEARQRLNNVKLVKPELAESLENQLIALAQAGRIRVPITDDELKEILAQVAEQNKRDYKIQIRERGWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F4B483</accession>
    <name>F4B483_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="10">GAPDH</shortName>
            <ecNumber evidence="10 12">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gap</name>
        <name evidence="14" type="ordered locus">Ahos_1308</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10 12">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10 12">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10 12">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="10 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776107.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B483"/>
    <dbReference type="STRING" id="933801.Ahos_1308"/>
    <dbReference type="GeneID" id="10600790"/>
    <dbReference type="KEGG" id="aho:Ahos_1308"/>
    <dbReference type="eggNOG" id="arCOG00493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
    <dbReference type="OrthoDB" id="295712at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18127">
        <property type="entry name" value="GAPDH_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02278">
        <property type="entry name" value="GAPDH_II_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003251">
        <property type="entry name" value="PRK04207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="4 10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="5 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="13">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="193"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="340" mass="37299" checksum="B68DF4389CF0DB78" modified="2011-06-28" version="1">MIKVALNGYGTIGKRVANAIMLQPDMKLIGIAKTSPNYEAFIAQKMGINIFVPQESIKSFDEAGIKVAGNIEDMIKEADIVVDATPNGVGAQYKELYQKFGKNAIFQGGEKANVADVSFSSLCNYNEAIGKKYIRVVSCNTTALLRTICTLNKLSKVEKVRATIVRRAADPKEVKKGPINALVPDPASVPSHHAKDVNSVLKDLDIITMAVIAPTTYMHMHLLNVTLKSQVSKEDVINQFLNTNRFLLVRGKSKVNSTAEIIEVARDLGRIRNDIPEVVIFEDSIYTMGNEVFLMYAVHQESIVVPENIDAIRASLNLASAEDSIKITNTSLNISKGYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B484</accession>
    <name>F4B484_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="5 14">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_1309</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="5 14"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B484"/>
    <dbReference type="STRING" id="933801.Ahos_1309"/>
    <dbReference type="KEGG" id="aho:Ahos_1309"/>
    <dbReference type="eggNOG" id="arCOG00496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000012">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 14 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE94192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="330" mass="35884" checksum="88451E3BCD7188DB" modified="2011-06-28" version="1">MSKNLDMNVEFVEDVMGPYARERIKNMKSGEIILLDNVRFVSEELIEAPPIQHAKSYLVKRLSPLFDGYVNDAFATAHRSQASIVGFPMVLKSTAGRVMEKEVSALAKIFNADEFPKVFVLGGGKVLDSIRIIENLVKRRLADRILTGGLIAELFAVAKGLDLGKENMQILEKKGLISLIPRARKILLSGGPIEIPVDFKVEKEGGKVAEEPSNKVTGVIKDIGSTTVGIYSSFIKDAKVIVMRGPMGVIEDERFREGSKKLLDSALESQGYLIIGGGHMISMLGGSSEINSSKVHVSTGGGALLLFLAGERLPALEALDLSYKEVVKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E9</accession>
    <name>F4B4E9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774955.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4E9"/>
    <dbReference type="STRING" id="933801.Ahos_0145"/>
    <dbReference type="GeneID" id="10599585"/>
    <dbReference type="KEGG" id="aho:Ahos_0145"/>
    <dbReference type="eggNOG" id="arCOG02792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055959_0_0_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051084">
        <property type="entry name" value="H+-coupled_symporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43528:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="442" mass="48674" checksum="E20C7662D288DA85" modified="2011-06-28" version="1">MDFSEIDFSKWTKAHWSLFSSLAVGFFMWGIIASIAPIFYPQVNQVWFLVVPIIAQLVGDLAISRLSDIRLGRKGTFFLTMGLYGTGALIIFGVSQAVVSGVISTSSPVFLPLVVLGIILGYLGIEGEVPTALSYAGETMPLSVRETMLVFLPNFDNMGAMVAAAISYLTYYLSSSYLLELRTLGILAIVMVGFAIVMRYLTPESVRWLVKAGKREEAEKEIEKYFSGAEVKHVTPRGVNEVSLGFRYAFLSIIGVSQYLTYGLMAYTIADYYFNGIVVDEIVLIANLGASIAGIIASFLVKYIPTRKFAVFSFLGGTLTMIPILLLISGVLPFSLGMFYVLLLFNMAFSEFGWATRTVLEPLLMPIKSRAFYIGLIRVAPMLAYAGSLYLTSGFSSTQFVIYNLGLWALGLGGSLAWYYKGYDTNMIPIEETAGDTTYRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F9</accession>
    <name>F4B4F9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774965.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4F9"/>
    <dbReference type="STRING" id="933801.Ahos_0155"/>
    <dbReference type="GeneID" id="10599595"/>
    <dbReference type="KEGG" id="aho:Ahos_0155"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="297"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="381" mass="45035" checksum="28D3B610F1F3FD5A" modified="2011-06-28" version="1">MARRVKAIRATVSMKIALSEPLLALVNNYVKAIRFTLFWLKENVPNPNEKGVLSKVHEALYEKLREEYNLPSKVAQDCYREALATYKGWYNNPRRGRFPRVYKPTVWLTPRASYNVDFEAMTVRIASVGELSILGYPRNLRDYLSWRMREARLVIRNDKAFLKVVFEEPRESVEPKESIAVDINMAEIVVGKDDTHYVRIPTRLDEVHHWKSLAENLQRKYPRRWRENKRILYRVRSFHLKARRIAEDFARKVGKWVVEIARMIGVNVIKLESLKNLIKNVDKLPKEFHDKLYLMQYRRLQYWISWQARKHGMIVEFVNPSYSSVSCPKCGQKMKEVSHRWFKCSCGYENDRDVIAIMNLNGRGSLTLSTVPQMRNVRANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G8</accession>
    <name>F4B4G8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774974.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G8"/>
    <dbReference type="STRING" id="933801.Ahos_0164"/>
    <dbReference type="GeneID" id="10599604"/>
    <dbReference type="KEGG" id="aho:Ahos_0164"/>
    <dbReference type="eggNOG" id="arCOG02560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_354376_0_0_2"/>
    <dbReference type="OrthoDB" id="43976at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051200">
        <property type="entry name" value="Host-pathogen_enzymatic-act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011964">
        <property type="entry name" value="YVTN_b-propeller_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02276">
        <property type="entry name" value="beta_rpt_yvtn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197:SF3">
        <property type="entry name" value="DIHYDRO-HEME D1 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197">
        <property type="entry name" value="PROTEIN NIRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="Nitrous oxide reductase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="769"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="792" mass="85695" checksum="AEC1B59036F910EA" modified="2011-06-28" version="1">MVIVNKILIITLVLLYVVSIISIIPITSATQGSKTTNISVGSFPTAMAYDSNNGYVYVVDSGSGQVSVISSSTVIATISVGSDPTAIVYDKQNGLIYVANTLSNSISIINRTEVIATVTVGGGIGYSPVALLCVNGLIYVANLNSYKAGVGIVSVINGTKVVCNIQVECHPVCLVYDPSNGYIYVSDTGFATVSVIKGEKVIATISVGQAPRQIIYDPYNGLVYVLNLESNSTSVINGTSVICTISVPYPVAIAYSSSYVYIVSLCNNTVYMISGTKVVGDITVGQSPVFITYDSYNGLVYVANLGSNSLSVINSSRVICQIPTGNSPRFILATPSAVYVADSGSNEVTEISVCKIIYYLTFKESGLPVGKSWIVCVNGTNETSTSNEITFILPPGMYFYKVYSAYYIPSPPQGSITLSSNATIQISFTPVKFNLTFEETGLVSGTKWAVCVNGTVSTSTSSRICFLLPYGYYEYKVLNVTGYKVTPIMGYIDLTSNTTIKVAFYAMLYNITFKETGLPKGVSWTVCIDNENHTTTACYITITLPMGVYEYKVFSSHYTPNVTQGTINLTMNKVINIKFTVMNYTLTIFESGLPSGYEWTVNINGSNYTTTMNEINITLPYGEYMVKVYSKYYMPITEEEFVNLSKNTELQFTFVPENFTITFEETGLPSGTTWTVCIDGRNYTTTSSSLTITLPYGTYEYRIFVTTNGYSPNVTQGTLTLTQTVKISITYKQITTTSTTTTSTTSTTTPSITTTPPPTTVTSPTLNTAFVVGIAIVVVIVIAAAIILLRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K6</accession>
    <name>F4B4K6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="9">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trpA</name>
        <name evidence="11" type="ordered locus">Ahos_1353</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776151.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K6"/>
    <dbReference type="STRING" id="933801.Ahos_1353"/>
    <dbReference type="GeneID" id="10600839"/>
    <dbReference type="KEGG" id="aho:Ahos_1353"/>
    <dbReference type="eggNOG" id="arCOG01086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_2_2"/>
    <dbReference type="OrthoDB" id="25658at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009621">
        <property type="entry name" value="PRK13125.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406:SF1">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE ALPHA CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE, ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="244" mass="27448" checksum="E4F307E1782C8F07" modified="2011-06-28" version="1">MSKMLVSYATLGYPKEKEYLEFIDGCVKEGSDILEIGVPPKYAKYDGPVIRRSYKQVSSWLKDIWPLLKETRDRVKIPIIILTYLEDWLPVLDDFLSNLAKIGIDGILFPDLIIDYVDEYEKYVKKIKDHGLKAVIFTAPSVPDPLIQKVSKISDIFLYYGVRPTTGVPIPVSVDSLITRIRILVDNKLVVGFGLSDVEDMRKALRAGADGIAIGTAYIEAIEKGGVQDAINLVKYFRGVLNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N5</accession>
    <name>F4B4N5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776180.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N5"/>
    <dbReference type="STRING" id="933801.Ahos_1382"/>
    <dbReference type="GeneID" id="10600870"/>
    <dbReference type="KEGG" id="aho:Ahos_1382"/>
    <dbReference type="eggNOG" id="arCOG00017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133047_2_1_2"/>
    <dbReference type="OrthoDB" id="42697at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730:SF3">
        <property type="entry name" value="HTH CRO_C1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40730">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="124" mass="13922" checksum="CF9B784DDA59A02F" modified="2011-06-28" version="1">MSITPPCEISVKEILPAVRSIIATKLVKEKGLPLYEAAKKMGITPAAVANYMNKKRGNSVRELIEHDKKMMEMITDFVEKVYIGTNEDLSNYYCMLCSEGKKVLKRSGIDVPLCAYESSLMLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N7</accession>
    <name>F4B4N7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="6">Glu-ADT subunit E</shortName>
            <ecNumber evidence="6">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gatE</name>
        <name evidence="8" type="ordered locus">Ahos_1384</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776182.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N7"/>
    <dbReference type="STRING" id="933801.Ahos_1384"/>
    <dbReference type="GeneID" id="10600873"/>
    <dbReference type="KEGG" id="aho:Ahos_1384"/>
    <dbReference type="eggNOG" id="arCOG01719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030702_0_0_2"/>
    <dbReference type="OrthoDB" id="7316at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.30:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003107">
        <property type="entry name" value="PRK04028.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="7">
        <location>
            <begin position="488"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="635" mass="71309" checksum="6EB356DD64D1BD34" modified="2011-06-28" version="1">MIDYNKLGLKVGLEIHQQLDTSTKLFCNCPTILSEEYKNTLERYLRPVFSETGEIDVAALFEWEKNKKYVYRIPQQATCLVECDEEPPHRMSEEALLIGLAMTLAFHGTPVDEVYVMRKVVIDGSNTSGFQRTSIVGLGGYVEDEDGKVSIQTIAIEEDAARKIEDTKDSVIYNLDRLGIPLIEISTGPDIHSPEQAKRVALKIGQMLRLTGRVKRGLGTIRQDLNISIIGGVKTEIKGVQELDLIPKLIEYEAMRQLKLLEIKDELIRRGATKENISSGYVVKDLTELFKETKSNVILRELKKGGKVYGIKVPHFKGIFGIELMPNRRFGTEVSDYVKVLAGLGGIFHIDELPNYGISQEEVNKVKDELNVKDDDGFILIVGEKSKLDLAVQVIKDRLLYALEGVPKETRGANEDGTTRFLRPQPGAARMYPETDIPPIRITEDLIKKAKEIIPPTPEEKLKSLINLGLSEELAKQVLNSPRLDSFDYFVKKYPKVPPVVIATTLENTIKSLKSQGGDPEAITDDVLESIFNALNSGKISKDSIPEILLKYSKERKEGENVNIDKIITIFSSLSEEELEKIVKETVETSKEEIIKKRDKAFNILMGKVMSKVRGRADGKRVADLIRRELEKING</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P0</accession>
    <name>F4B4P0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C/D box methylation guide ribonucleoprotein complex aNOP56 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1387</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776185.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P0"/>
    <dbReference type="STRING" id="933801.Ahos_1387"/>
    <dbReference type="GeneID" id="10600876"/>
    <dbReference type="KEGG" id="aho:Ahos_1387"/>
    <dbReference type="eggNOG" id="arCOG01923">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015495_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000007">
        <property type="entry name" value="Pre mRNA splicing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048896">
        <property type="entry name" value="Nop5_56-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047099">
        <property type="entry name" value="Nop5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011121">
        <property type="entry name" value="PRK14552.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21572">
        <property type="entry name" value="Nop5_56-rel_N_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Nop" evidence="3">
        <location>
            <begin position="257"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="409" mass="45754" checksum="C15CD284A77D008F" modified="2011-06-28" version="1">MKLYLVEHAIGTFAFDEDGKLVDYVLNPKDIGKLVEILLNHEKGEPFPSTLELISKLKPEEVVIENEAEIPKLKVKASATLNHKAAKMFRSSLVKFALDSKFASSEDELYNFLYQLSFEYTRRKLRTAAEKRDLLAIQAVRAMDDIDKSINLFSERLREWYSLHFPELDKLVEDHETYAKIVSTFGYRDNITLEGLKSINIDEKQAKKILDAASKSIGADISEDDINSIKQLSDAILSLYNIRNSLSDYVESVMKEVAPNITALVGANLGARLLSLAGSLEEFAKMPASTIQVLGAEKALFRALRSGGRPPKHGVIFQFPAIHSSPRWQRGKIARALAAKLAIAARVDNYSGRFIGDQLVEQLNKRIEEIKTKYAQPPPRKPQPQAPKQKSFKKGGGGGKKNKGKKGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q8</accession>
    <name>F4B4Q8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name evidence="2" type="ordered locus">Ahos_1405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776203.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q8"/>
    <dbReference type="STRING" id="933801.Ahos_1405"/>
    <dbReference type="GeneID" id="10600894"/>
    <dbReference type="GeneID" id="42778464"/>
    <dbReference type="KEGG" id="aho:Ahos_1405"/>
    <dbReference type="eggNOG" id="arCOG02726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_4_1_2"/>
    <dbReference type="OrthoDB" id="35548at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.60">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000684">
        <property type="entry name" value="PRK00034.3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="11166" checksum="10254C4D0B223413" modified="2011-06-28" version="1">MKIKVDDQLIEKLQRLALVEINDNEKEIIKNDISKILEFFNKINELNLENVDPLFHPIPQGKLRKDEIKTPLDRDSALQNVKRKENGYIIGPSTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U6</accession>
    <name>F4B4U6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypA</name>
        <name evidence="5" type="ordered locus">Ahos_0219</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <text evidence="4">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
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        <text evidence="4">Belongs to the HypA/HybF family.</text>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <feature type="binding site" evidence="4">
        <location>
            <position position="2"/>
        </location>
        <ligand>
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    </feature>
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        <location>
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        <ligand>
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        </ligand>
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        </ligand>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="148" mass="16879" checksum="143FD60BF55ABEA9" modified="2011-06-28" version="1">MHEWSVADAVIRTVINWVDDKKIKVKKVVLGVPSFSFLDVEILKEAFNIMKRDSVLDEAELEVKFKDLRFKCKNCGKEFSLNDVKDQIDSVRSEFGEEYPMHFMPALAPSFIKCPYCGSHDIALESQDMTIDEIQVEENGTVKTVSQG</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0238"/>
    <dbReference type="GeneID" id="10599678"/>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="129"/>
            <end position="150"/>
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    </feature>
    <feature type="domain" description="TQO small subunit DoxD" evidence="2">
        <location>
            <begin position="12"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="19492" checksum="116C330C254E62F9" modified="2011-06-28" version="1">MGQVVIETTQKYLPILRVTLGWMFFSAFVRRTINVPAKLNPHSSAYVGGKLITFLPHAWGPVKGVLLWVLQNPPLLYAFLIMFTALEAIFGLFMMLGFLTRPSGLILAGLAWGIGFGGGWLGSTCVDEWQIASVEGAAAFMFVFTGSRWLSIDQLLYKKYNKGIKIWKFNIPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Y4</accession>
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        <recommendedName>
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    <organism evidence="2 3">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Y4"/>
    <dbReference type="GeneID" id="10599698"/>
    <dbReference type="KEGG" id="aho:Ahos_0258"/>
    <dbReference type="eggNOG" id="arCOG01197">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_4_2"/>
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    </dbReference>
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    </dbReference>
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            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="115" mass="13470" checksum="AF1AFE5B8F5F0F0C" modified="2011-06-28" version="1">MGKGKSAIESQIRMINLVKEIVEELACDFANLEEVYIFGSRARGNYLDTSDIDVIFVFKGIKGMNVFDRMYMVSKYIKGNVDYIVLDEEEKDRIREKKLLWNKDKGFVDMKDFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z9</accession>
    <name>F4B4Z9_ACIHW</name>
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        <submittedName>
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    </protein>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="AlphaFoldDB" id="F4B4Z9"/>
    <dbReference type="STRING" id="933801.Ahos_0273"/>
    <dbReference type="GeneID" id="10599713"/>
    <dbReference type="KEGG" id="aho:Ahos_0273"/>
    <dbReference type="eggNOG" id="arCOG01195">
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    </dbReference>
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    <dbReference type="OrthoDB" id="373082at2157"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="102" mass="11625" checksum="0C11716313410625" modified="2011-06-28" version="1">MEKSFFVQLAEKIRPTFGKVAIILHGSRIKGNYYPASDLDVLIISEKCLGRGGLRILYSLGEELNGVKLDYQLVCYKNKDFWIVKNVLSSPHLVVVDDFNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B579</accession>
    <name>F4B579_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease-associated region</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0282</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775089.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B579"/>
    <dbReference type="STRING" id="933801.Ahos_0282"/>
    <dbReference type="GeneID" id="10599722"/>
    <dbReference type="KEGG" id="aho:Ahos_0282"/>
    <dbReference type="eggNOG" id="arCOG03463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="OrthoDB" id="41965at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="53647" checksum="A4DB3AE56144F187" modified="2011-06-28" version="1">MKFLRESTGLVKEFSAVDAFALNFSFLGPAAGVSYPLFVSAFLPNSSWILSVILGAVLMIPLVLNYYLLNALIPRSAGDYIYVSRAFGPFAGSILGMSLLMSFMMGFPVLAMLEVIMVIVPGLQAIGYSLGNEALINFANSILSSSLNLFVTTTLIVLIAFLLSSSERYYARTFRYLTFLQIIGTVTMIYGLLTFHYSGVSLDPKLNTQTFALSSIFVLSLFAFANAPSFFAGEIKKNRKSFLAGYLLSYTVATIFVLLLIISLEIGIGKENYVSAVISGWNLPISTSSLLSFGVLPFIHYPAIVMIIFVTALSWYLLYAMINVGASSRILFSLSFDRLLPAFLADVKRGVPFNALLLSFLVSMIFNYVEVYLGYSISFAIDGLWFVIWNYLIVAIASIKFYKLDKRVLYTSISSVIALSAVVFTTLYYGIVNSTFGGVIFEGNFAFDIISIFIPPITGAVTFVISQKVRSKQGIKLSLIYKEIPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B595</accession>
    <name>F4B595_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775105.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B595"/>
    <dbReference type="STRING" id="933801.Ahos_0298"/>
    <dbReference type="GeneID" id="10599738"/>
    <dbReference type="KEGG" id="aho:Ahos_0298"/>
    <dbReference type="eggNOG" id="arCOG03166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_671970_0_0_2"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704:SF1">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="398" mass="45599" checksum="6720B48E2EFD0260" modified="2011-06-28" version="1">MEILRREYNDVKNFSSWTLVYGRRKVGKSFLVRKYVNYDLYYVITRDLQAYFPKDNSFRNISEAFSLTIKALRENKTVVIDEFQRMPEKYWDGLSVEHPNGKLILVGSSFRIMQKLVDRQSPLLGLVLPYNLGIIHYSDVLSQVGDPLLSLIYRDPWTLSFVKSVKDLEDKAYQLYMVTKALIGEIFEEEERQLTSLYEAILLSLAEGEWNTVIIAGSLNSKGFNVTPSSVSGYLETLQNMGLVEKIPIYGEKKRARWYYKISSPIISLIFYAEAKYNVSFTENVGELPIGREVQFSIGELLAEKHNATLAYSPYEDIDIVLLEKGKPIIGYEVKLGEFSKAEAEKSIERIRRAGIPKAGLISLKEKPNFNADEELGPEDLVRISRDVFQKYISKGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B598</accession>
    <name>F4B598_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="6">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_0301</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775108.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B598"/>
    <dbReference type="STRING" id="933801.Ahos_0301"/>
    <dbReference type="GeneID" id="10599742"/>
    <dbReference type="KEGG" id="aho:Ahos_0301"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_0_0_2"/>
    <dbReference type="OrthoDB" id="30755at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000049">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053399">
        <property type="entry name" value="2-oxoacid:Fd_oxidored_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011896">
        <property type="entry name" value="OFOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032686">
        <property type="entry name" value="PFO_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041171">
        <property type="entry name" value="Oxoac_fdxbeta_Archa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02177">
        <property type="entry name" value="PorB_KorB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF2">
        <property type="entry name" value="PYRUVATE FERREDOXIN_FLAVODOXIN OXIDOREDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12367">
        <property type="entry name" value="PFO_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="45"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate ferredoxin oxidoreductase beta subunit C-terminal" evidence="15">
        <location>
            <begin position="195"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE93192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="297" mass="32965" checksum="EA91319F63C74D8D" modified="2011-06-28" version="1">MQVTEWNDWCPGCGNFGILNAEQQAISELGIDLKKVVLVSGIGCSGKLPHFVRIPVSGVHTLHGRALPFAIGIKLANPELEVIVNAGDGDQLGIGVGHFVSAGRRNVDITVIVHDNGVYGLTKGQASPTLKKGVKTKSLPKPNINDDINPLTVALASGYTFVARGYAYDVKHLKELIKEAIKHKGLALIDVLQPCPTYNDINTKEWYDKRIYKLDWDPVVRNEEDKKKKFLQAMEKALEWGDRIPIGIFYKEERDTFEERISSASPSYKILPPAKVKIEKEGKPTTIIDEILKEKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D0</accession>
    <name>F4B5D0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0333</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775140.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D0"/>
    <dbReference type="STRING" id="933801.Ahos_0333"/>
    <dbReference type="GeneID" id="10599774"/>
    <dbReference type="KEGG" id="aho:Ahos_0333"/>
    <dbReference type="eggNOG" id="arCOG03663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000631_7_2_2"/>
    <dbReference type="OrthoDB" id="27033at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06604">
        <property type="entry name" value="GH31_glucosidase_II_MalA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053497">
        <property type="entry name" value="GH31_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040948">
        <property type="entry name" value="alpha_gluc_MalA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF120">
        <property type="entry name" value="HETEROGLYCAN GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 31 N-terminal" evidence="6">
        <location>
            <begin position="47"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="5">
        <location>
            <begin position="167"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="7">
        <location>
            <begin position="524"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="684" mass="79475" checksum="B3FCB227D5830633" modified="2011-06-28" version="1">MQTIIEENEGIYRIKVNNPIPPVDFNFKGKVSNKSLSDFSIHIKESEGYIIIEKPLELKEHIVGLGEKAVELDRKRKRYIMYNVDAGAYQKFQDPLYLNIPFMISIYEGKATGYFVNSASKLIFDIGFEDYGKVKIKVPEESFEFYILEGSTIEKVLERYAELTGKPFLPPIWAFGYMISRYSYFPQERIIELLDLMQREGFNVTGVFLDIDYMDSFKLFTWNKERFPDPRRFIDEVHSRGVKVITIVDHSVRVDQNYEVFISGLGKYCETDKGDLFVGKLWPGNSVYPDFFREETRDWWSELISKWLSQGVDGIWLDMNEPTDFTKVYEILDIFKETPIQIKDDRFYTTFPDNVVHEIKGNKVPHPKVRNAYPYYEAMATFEGFKKAERDEIFILSRSGYAGIQKYAGVWTGDSTSSWDQLRLQIQMVLGISISGIPYVGIDIGGFQGRGLKEIDNSPEMLLRQFQLALFFPFYRTHKATDGIDTEPIFLPSYYKEKVKEAINVRYKFLPYMYYLAKEAHETGHPIVRPLFYEFTEDEDTYRIDDEYMLGKAVLYAPIIYPQVNTRKVYLPKVKWLDFWTGKIVEGWIDSSTDLPIYIREGSIVPLSEGDMIIYGNSELKYDNVTIRSEGNLITFSKPFRLGKVTIITDSNKVIVNGKEYEGTRQGNAIVVNINSEVSQMRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E1</accession>
    <name>F4B5E1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cellobiose binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0344</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5E1"/>
    <dbReference type="STRING" id="933801.Ahos_0344"/>
    <dbReference type="KEGG" id="aho:Ahos_0344"/>
    <dbReference type="eggNOG" id="arCOG01535">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025114_0_0_2"/>
    <dbReference type="OrthoDB" id="194307at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF9">
        <property type="entry name" value="OLIGOPEPTIDE-BINDING PROTEIN APPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="632"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="88"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="653" mass="72413" checksum="9A7F800D5310C0A0" modified="2011-06-28" version="1">MSNQKLLKYISIVVVLSLIIIPAASSALSLAATSSCPTLYLAYVTSSPYQSLSTFNPNLFDGGLGGDFYGLIFGYTITLNVSNNEVIPCLISNWTFSPSNWEQVWQNETVNVTITLRHSGWANGQPVTAYDILATCLILDMYSAPPYPNYTIVNNYTIIISEPPGRMSPVLLSHTLICSIGLGEVALIMSYQQYKPLIQQIESDWTQLQHNNVTLIKEFRDKIHSYVPPGPISANYNGPYYVASITPSEIVLEKNPYYFAASNVKFNKIIVYQYQSTSDFYAALFKGQISLDLPSVVSVCPKVLSELPSCYKVISIPNPGGWALYFNFKNPWLKMVQVRQAIAYVLNRTSIALAGGYPKFTPVKVPNGIPCCFSYLKQFITPAVSNLNPYCTNLSKAKQLLESVGFTCKNGQWYTPNGTPFTLTILEPFTLSPCVYNMMNVIKDELTAFGIPTTYYVCTNVKVNHEHYDTGTGYDLVFQHWGGFCIGTVDWYLETQYLNGIPLNVTQWDGIVTLPNGTQVNVVKLYGETVSPNSTSQLIAANDEMAYALNYYLPALPLVFQSCQIVVNTKQLNVPPSNSWFWQEYFYGIAGTAIYQLGFTDGFIQPVIVTTTPPTTTPPTTTVTSTLPVVDIAVGVIVVIIIVAAVVIIMRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E3</accession>
    <name>F4B5E3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bile acid beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775153.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5E3"/>
    <dbReference type="STRING" id="933801.Ahos_0346"/>
    <dbReference type="GeneID" id="10599787"/>
    <dbReference type="KEGG" id="aho:Ahos_0346"/>
    <dbReference type="eggNOG" id="arCOG03867">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010759_0_0_2"/>
    <dbReference type="OrthoDB" id="40125at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006775">
        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024462">
        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12215">
        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="347"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="549"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="840" mass="96758" checksum="2DB834397E1355EF" modified="2011-06-28" version="1">MKYSFTYNISSGIPLGGIGTGSVEIRADGRLYEWTIFNNGGYAERQDLRFTYYLDEQDSFFAVRQKGIVRVLQAFDYNFGGSPYILPWLRPVERVEFNGEPPIAYLEFVDKISVNMKAFSPFIPTDLKNSSLPSAIFTLESNEESDFMFGIKNPFNEGNVDFKDNTLILSGEVEPNDPRYQGNLCIKILAEYPFARVIERHPSEEFQLWHEFRENGRLENKLGKGNFALIGGKGRKVTFILSWYFPNHLLADGRRIGHYYENFFSNCLDVAKYVESNLTYLESKTTAFHDLLYNVKGIDSWIADLVGSQLTTLIKSTWLAKDGFFGIWEGYFNTADKRKTANQYPYTDGPLHTALNTIDVLTYSLPTILNLFPELAEKIILQFKDKLIEENTPEHVVYSLSFEENREKFLEKLSKDPSLPTDGEKLYSTINEIVKETGKDPKGRVPHFFTDNLRVDEYHRVDLNPEFILMTYLIAKTTGDLNFLKEIFPKMKEALESTMKTQTYDGLIYHTLPAGLEWLRYVNNKLNLPRGDNNSASILGHNLIPLSMQTFDDWSMIGITSFTSILWISSIQAVNDACSNLKINCSYDYESLVKKLIDYLWNGEYFDLWYDPKSKMRDKACNASQILGHWYSTLLGLRFLDDSLVKTTLKSIVKYNLKEEEGLLNGAYPNGYRPLKRNYQNQLNLPATTQIDTPWSGVEFYVASHLIYEKLRDEGEKILRNIYERYKLAGNFWNHLEWGSHYLRPLDSLMVIHAYEGLKYDGITKTLTIDPAVEELSWIIFLPTGWGKIEISKQKVSIKMFHGVLEINKLKAEPKKVLLNGKEANLPIELKEGDSLDILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5F9</accession>
    <name>F4B5F9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1500</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776298.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5F9"/>
    <dbReference type="GeneID" id="10601000"/>
    <dbReference type="KEGG" id="aho:Ahos_1500"/>
    <dbReference type="eggNOG" id="arCOG05380">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053359_6_1_2"/>
    <dbReference type="OrthoDB" id="57223at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="111" mass="12144" checksum="9F4DDA20492D29F2" modified="2011-06-28" version="1">MGSPSDHLANERTFLAWVRTGIALIGFGFVIAKFALFISILKGAKTLSSSSVTYGEVMIILGGIVILYGLYSYLENEKDISKNQYTPRVLPNMLFVGIILAITVILALFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I8</accession>
    <name>F4B5I8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1529</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776327.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I8"/>
    <dbReference type="STRING" id="933801.Ahos_1529"/>
    <dbReference type="GeneID" id="10601029"/>
    <dbReference type="KEGG" id="aho:Ahos_1529"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="11"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="13324" checksum="B86D5979DFD5704F" modified="2011-06-28" version="1">MLDKEEYERWIVSAKQTLESARADLNSGFYDWACFKSQQAAELAVKAYLYGIGQPKAGNSISYLLSLLKAPQEIIDKAKHLDKLYILTRYPDAWSSSSPSYTKMRLKRQLNMLKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J3</accession>
    <name>F4B5J3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776332.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J3"/>
    <dbReference type="GeneID" id="10601034"/>
    <dbReference type="KEGG" id="aho:Ahos_1534"/>
    <dbReference type="HOGENOM" id="CLU_738893_0_0_2"/>
    <dbReference type="OrthoDB" id="43006at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="374" mass="41441" checksum="2EDFF416273C3945" modified="2011-06-28" version="1">MEYIRLEGIIGLIIVIGILLTIVAMGPHFSSTMQTGPSKFKVISAELLCVCKNYIIKATIEASGIPGNCHDNLYEVVLNGTTYYAFYKESNDGQVVWYHNGTHTYEIYLSTSNTTYSPPTNLPKGTYEATFISQQGKCTITVSFGVAPKPQLKIVQAEILNVSGLYVLKTEVNASIPVGYIYFYEAILNGTPYYIFYHPKGYTLCPVYYIDKSGVYNVSLYIYPKNFTAPTNVTPNSFEIKPGIYEVTFVTSIGNYTITAKTATTTSTPQFSVENTAKEGVVNGLTQLNFTITATTPLGGHICVYEICVNGHNYTVYYEPKVSTSAIGVYVEQGTGTYCFYINGTISAGTYTIKFYYCYEGVHYVYSEEINIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M3</accession>
    <name>F4B5M3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fe-S oxidoreductase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1564</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776362.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M3"/>
    <dbReference type="STRING" id="933801.Ahos_1564"/>
    <dbReference type="GeneID" id="10601065"/>
    <dbReference type="KEGG" id="aho:Ahos_1564"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="OrthoDB" id="30736at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04053">
        <property type="entry name" value="TIGR04053 family radical SAM/SPASM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="4"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="351" mass="39307" checksum="31A3D22CF8A37250" modified="2011-06-28" version="1">MSFERAPHLVFWEVTKACPLACKHCRANAIQDPLPGELTTAEGKKLLEEISTFGKVVVVFTGGDPLSRDDIFELMDYAKQLGLVTSIAPAPSYKLNEDTIRKIKESGVTYMSISLDGAKPETHDWLRGLTSYKYAINGIKEGLKQGLIVQVNTLIWKGSYPELPQIAKILHDLGVKVWEIFFLIPVGRGSIELDIPKENYKKVVNFLLEVSKYNIIVRTVEGPFFRRAKLEYPEGFEDNELIEELRKLLGKPPKMDVDKSIVPTRDGSGVIFISYDGEIYPSGFLPLSLGNVRKDNIVKIYRESHLLKLIKEGKLKGKCGSCKYVNVCGGSRARAYAVYGDPLEEDPACPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N3</accession>
    <name>F4B5N3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775164.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N3"/>
    <dbReference type="STRING" id="933801.Ahos_0357"/>
    <dbReference type="GeneID" id="10599798"/>
    <dbReference type="KEGG" id="aho:Ahos_0357"/>
    <dbReference type="eggNOG" id="arCOG03847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514679_0_0_2"/>
    <dbReference type="OrthoDB" id="37074at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10770">
        <property type="entry name" value="Hypothetical protein VC1899 like domain (Restriction endonuclease-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040581">
        <property type="entry name" value="cas_Crn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19621" checksum="C4D45D8C8F926AD4" modified="2011-06-28" version="1">MPKLVSTLGKSPGGIAETLVSLTEGNYVTPFDPVPIKIEELIVVKTKEVEESFYVLKALLLCCSNFVNVKVINLPFDDVNSPSDFVQVRETIRKVLKAGDYLDFTGGRKAISSAAVLAAREAGAHLVSSIIPHEEYEIINKKFNAVKDRAMKIYKKEDCVSYVCDLISKDSRTIVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5U2</accession>
    <name>F4B5U2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="376419at2157"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="76" mass="9219" checksum="EC44EEB6B84E492F" modified="2011-06-28" version="1">MEDDLTQKLISLVEEYKEVNYKAQEIFKELKLLMRKGAINPWRVNIMDSEGKFIDEYLNEIEKELEIEKEIRLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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    <dbReference type="KEGG" id="aho:Ahos_0426"/>
    <dbReference type="eggNOG" id="arCOG07734">
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AEE93314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="174" mass="20250" checksum="128A31763792EB72" modified="2011-06-28" version="1">MEEEFAKFLEDYANYLKNNKQPLIDIPLSPDDLLAEASRIRAKSRVKLKDRRIIIQLTNGEEKHWAHIEGEIIMTFDKLYRPLKVEIEIKDVMDSEKVLKNLKSEKISDIEFVTDNEFIEIYLANGEAEHWAHFEGEIVMTLNDSYTPLRLEIEIKDVMDSEKVLKNAGLIPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="STRING" id="933801.Ahos_0433"/>
    <dbReference type="KEGG" id="aho:Ahos_0433"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0070573">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Metal-dependent hydrolases"/>
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        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF12">
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    </dbReference>
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    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AEE93321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="312" mass="35364" checksum="92188F1D1424483C" modified="2011-06-28" version="1">MVHMFIDLHEDFAYANQMGRDIIHGKGQSSIEMLKNVDSNVIIFSSIFPHIDVINERSAELSSKYGFPIKSSNFSFDILLSQLKVYYYLERAGYVSILRSKEDLKKPGIKFLLSLEGTDVLKEYEDIYILKELNVFNLGLTWNYDTKFASSCFSKKDYGLTAEGEELVKLANKLGIIIDLAHAGKRTVLDVCSVTSKPVIISHGNAKRLKDHERNYDDEELECIVKTRGVIGITAITSTLPEKTLKGIIENIKYIGESFGWDYVAIGTDFLGISEVPQGFENVTKIKDLLIEGHEDQILWRNAYRVIEENLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Z2</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    <dbReference type="eggNOG" id="arCOG04033">
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="21972" checksum="7445991E2229AD41" modified="2011-06-28" version="1">MQTVEYPISTYLPRDVVYFDGNSATLPQDYVLKEGNLRLFVPKNKIEDVVNALKGSGFREEKLEFYKGEKYSLSMRIYNIWELHVRIHDDGFIDGHFEVSRDYLEHLQYYTIPSIYEVFDFYRTAYNKLHIFDNVAKKWIKEVRTQYLITLEPPKSLTAWQPITAIVGALSAIGIIAYALSRLDKGED</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B645"/>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="126" mass="14914" checksum="EFF8989D31B99A74" modified="2011-06-28" version="1">MSVYSTKSQIETWIKEADDLLEKGDIVQASEKYYKAVEEGIKLLVIKKGLNCIINEVIRKRGWKSEILYKASRRLSIILSENILKCWRDAYYFHTLGFHEMVLNVEKMKAKLKDFKYELEYLLSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B651</accession>
    <name>F4B651_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CHAD domain-containing protein</fullName>
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            <taxon>Thermoprotei</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="eggNOG" id="arCOG07254">
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24701" checksum="6D8405215F848B30" modified="2011-06-28" version="1">MKLKNLRPEDYANKNLRDALSISGISKEDIHDRRVKLRKYFDVLYSLYPVYENPDCLVKAKDLIHKLGVIRDMDVICYQPNRDKLAEIVSKKIRVMNYCFLLKVYGSRLLVFNRILSLQKKIEKIEDFHELRKIVRTTRNLVESLGYKNKELSSLAKEMGDLRDEILKIECEGRKVSIDVENYKKRANEIIVTFIIYQDEFHHFSLQQ</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10599896"/>
    <dbReference type="KEGG" id="aho:Ahos_0456"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="44"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="124" mass="14233" checksum="1BC426EE1CDF0A75" modified="2011-06-28" version="1">MDFYLSNISQVPKEDVPIKGTKGAKIQWLITKNEGAHYAVRKFSLEKMGVIPMHVHKYQETVIITKGKCKVCVADKEFELKEGDYIFIDSQVKHAIIAEDELEFFCVIDYVDDMSIKTLNEDCP</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B672"/>
    <dbReference type="GeneID" id="10599912"/>
    <dbReference type="KEGG" id="aho:Ahos_0472"/>
    <dbReference type="HOGENOM" id="CLU_2379213_0_0_2"/>
    <dbReference type="OrthoDB" id="376213at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10918" checksum="CEFAA4CD5D1B32C3" modified="2011-06-28" version="1">MLKEILNNSSISELLQQGKEIDCTREEFFSELDEIITKASAEGYKVEGPILSYDKGLNKLTYDVKKGDKKVGEISLYYGNFYRKYVQYVKFSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A9</accession>
    <name>F4B6A9_ACIHW</name>
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        <submittedName>
            <fullName evidence="7">Methylmalonyl-CoA mutase, alpha-subunit, chain B (McmA2)</fullName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775313.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6A9"/>
    <dbReference type="STRING" id="933801.Ahos_0509"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cobalamin-binding domain"/>
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        <property type="entry name" value="Cobalamin-bd"/>
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        <property type="entry name" value="Cobalamin-bd_sf"/>
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    <dbReference type="NCBIfam" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF3">
        <property type="entry name" value="COENZYME B12-DEPENDENT MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
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    </dbReference>
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        <location>
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            <end position="132"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93397.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="141" mass="15114" checksum="94316C05A746CC50" modified="2011-06-28" version="1">MEKRIKVIVAKLGLDGHDRGAKVVARALKDAGMEVVYTGLRQTPSQIVKTAIQEDADVIGVSILSGAHLELIPPLVNLMKENGLTDVGLIVGGVIPPQDIEKLKNMGVDEVFLPGSSLKEIVEKVKKVAEKKRGIKIGQTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B3</accession>
    <name>F4B6B3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfide:quinone oxidoreductase</fullName>
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        <name evidence="2" type="ordered locus">Ahos_0513</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775317.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B3"/>
    <dbReference type="STRING" id="933801.Ahos_0513"/>
    <dbReference type="GeneID" id="10599954"/>
    <dbReference type="KEGG" id="aho:Ahos_0513"/>
    <dbReference type="eggNOG" id="arCOG01065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_1_1_2"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="203"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="409" mass="45148" checksum="8456BD349422C724" modified="2011-06-28" version="1">MTKVLVLGGRFGALTAAYTLKRLVGSKADVKVINKSRFSYFRPALPHVAIGVRDVDELKVDLSEALPEKGIQFQEGTVEKIDAKSSMVYYTKPDGSMAEEEYDYVIVGIGAHLATELVKGWDKYGYSVCEPEFATKLREKIESFQGGNIAIGSGPFYQGHNPKPKVPENFVPNADSACEGPVFEMSLMLHGYFKKKGMLDKVHVTVFSPGEYLSDLSPNSRKAVASIYNQLGIKLVHNFRIKEIREHEIVDEKGNTIPADITILLPPYTGNPALKNSTPDLVDDGGFIPTDLNMVSIKYDNVYAVGDANSMTVPKLGYLAVMTGRIAAQHLANRLGVPTKVDKYYPTIVCVADNPYEGYAVSVKDDTWYGGTVSIADPAAVNHLKKELFTKYYMWTKGDMALEKFLASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C4</accession>
    <name>F4B6C4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF488 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776459.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C4"/>
    <dbReference type="STRING" id="933801.Ahos_1666"/>
    <dbReference type="GeneID" id="10601166"/>
    <dbReference type="KEGG" id="aho:Ahos_1666"/>
    <dbReference type="eggNOG" id="arCOG06027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137928_0_0_2"/>
    <dbReference type="OrthoDB" id="7940at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052552">
        <property type="entry name" value="YeaO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849:SF1">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36849">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22752">
        <property type="entry name" value="DUF488-N3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13727" checksum="1D683717476317BB" modified="2011-06-28" version="1">MIKVKRVYDPLEKDDGIRILVDRLWPRGVKKDQVDVWLKDVAPSDELRRWYNHKPEKWEEFKRKYFEELSRNPKVEILLQLIKKGNNVTLLYASKSPYNNAVALKEYLEKILKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H9</accession>
    <name>F4B6H9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1722</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776514.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H9"/>
    <dbReference type="STRING" id="933801.Ahos_1722"/>
    <dbReference type="GeneID" id="10601222"/>
    <dbReference type="KEGG" id="aho:Ahos_1722"/>
    <dbReference type="eggNOG" id="arCOG02688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF34">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="448" mass="49713" checksum="B9607CE99B118732" modified="2011-06-28" version="1">MEKSKISVVSRMERLPLSKIHYKFLLLVMGGEWVETLMLLGNGVIATVLSAVLFSSLSTAVTAITTAFFLGETVGSILFGRLSDLKGRRTVFLVNLLLFGGGTIIAGFMSNFYAISALMFIAGIGVGGEFPLVDTYTSEMMPGKYRGRGVALVYTLAVTSAPVIAIVTYFSSHPLNYYSWRIPLWFMGVAALIVWALRTRLDESPRWLENKGRHEEANKIVEKWENAVLKEGKVLPEPEKVEVVEQHSKISELFSKDLRTRTIMMLIFQFFQSGIFYGFVVLAPSFLLAKGISLVNTLLFTIIIDTGFIAGSVFNYFIIDRVDRKYGIIGSAVFAGILGTLFALVSNVYLTVLLGFLVAFTLWNFSNFFHTYQAEIFPTRVRSTGAGLVYSVSRFSTSILVLMITAFFLPRGLLATFGIIWVFIAIVSLDIGLLGPRTTGKKLEEISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K9</accession>
    <name>F4B6K9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable thymidylate kinase</fullName>
            <ecNumber evidence="8">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tmk</name>
        <name evidence="10" type="ordered locus">Ahos_0531</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775335.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K9"/>
    <dbReference type="STRING" id="933801.Ahos_0531"/>
    <dbReference type="GeneID" id="10599972"/>
    <dbReference type="KEGG" id="aho:Ahos_0531"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_3_2"/>
    <dbReference type="OrthoDB" id="43083at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF4">
        <property type="entry name" value="UMP-CMP KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="9">
        <location>
            <begin position="5"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="189" mass="22009" checksum="DB3050ACAD04E1E3" modified="2011-06-28" version="1">MLIAIEGIDGSGKTTLAKELKKWLENEKKKKVLLTAEPFTEEISKLIQEEGWKDPVTLTLLFSADRGVHVNWIMKQNQYDIIITDRYYYSTIAYQSAMGIDKNWIIEVNKFFPKPELTLLLDIPAEIAITRIKKDDKFNFKEKLSLLKKVRENYLEITKNENSIKIINSTKTFEEVLSEAKNYVEELIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L9</accession>
    <name>F4B6L9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">FAD-dependent pyridine nucleotide-disulfideoxido reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0541</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775345.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L9"/>
    <dbReference type="STRING" id="933801.Ahos_0541"/>
    <dbReference type="GeneID" id="10599983"/>
    <dbReference type="KEGG" id="aho:Ahos_0541"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="2"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="10">
        <location>
            <begin position="307"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="410" mass="44795" checksum="5AF5863B7F7A6C1A" modified="2011-06-28" version="1">MNITIIGSGPAGIYSAIASAKLGNKVKLVEKNDKLGGTCVLHGCIPSKAMLHPLFLKYFAEETGRELNFSFSEIQKIAKNAVNRLSKGVEYMLESYGIEVIHGKAELRSGNIQVGGQTIPSDKIIIATGTEKPQVEGTIASDDLPYLDKEFSKVLVVGGGAGGVEYAWLLKMSGKDVSIVEKSDNLLPYLDEDLKKAVTAYFKKIGIKLYLSSEITLGDKPRIGNEELPQYDIILYTFGRKPALDGFEELPHEKWIKVDKRMYTGVNNIYAAGDITGTFTAHEAIHEGFVAGLNASGIEKYYKPEAVPKVIYTEPQIAYVGNTKGKCVKINMAEIPRAIAEGLTEGFLKVCVEGKKITGAVAFSHDAENTITLISTFINYGIEIDKAIDFIEPHPSYLEAVFEALLRLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N6</accession>
    <name>F4B6N6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0558</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775362.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6N6"/>
    <dbReference type="STRING" id="933801.Ahos_0558"/>
    <dbReference type="GeneID" id="10600001"/>
    <dbReference type="KEGG" id="aho:Ahos_0558"/>
    <dbReference type="eggNOG" id="arCOG00695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
    <dbReference type="OrthoDB" id="372084at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01298">
        <property type="entry name" value="ATZ_TRZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="2">
        <location>
            <begin position="53"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="408" mass="46621" checksum="FCFF2B45222158AD" modified="2011-06-28" version="1">MEVFNKTYTLKNCSFVIDSEKILENTNIVIENGKIKSIGKETEGDEIDCSEYVVTPGFVNSHTHSAMIFLRGYYDDSELQEWLDKMWEKERSASKDVLKISSELAVLEMLSSGTTAFVDMYFNPEDIIELSNNYGIRAAAGYTFLDKIFDPEEVAKMQRRLRETNLFKPIINVHSIYAVNEKTLLLAKDLAEEQNTWINIHLSETRREIYETKLRKGKFPVEYLHSLGITKFQAVHLGWVASWEIEMLKDSVTVTHCPTSNMKLATAGAFPFYEMMNSGINVTIGTDGPASNNSLDIIREMKNAVLLQRHSYWDTRIKALHVFKAATENGYKLLGIKGGLIKEGYVADLVLFNKDDLYPLKKDRLLSNIVYYATGENVNKIIINGRIIDKKFIKEKIHELAKKLNEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R7</accession>
    <name>F4B6R7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1512 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R7"/>
    <dbReference type="STRING" id="933801.Ahos_0589"/>
    <dbReference type="KEGG" id="aho:Ahos_0589"/>
    <dbReference type="eggNOG" id="arCOG04181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062565_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009995">
        <property type="entry name" value="DUF1512"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056461">
        <property type="entry name" value="DUF1512_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056460">
        <property type="entry name" value="DUF1512_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07431">
        <property type="entry name" value="DUF1512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23542">
        <property type="entry name" value="DUF1512_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016495">
        <property type="entry name" value="UCP016495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1512" evidence="2">
        <location>
            <begin position="25"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1512" evidence="3">
        <location>
            <begin position="205"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="42187" checksum="892769A4BC68E03E" modified="2011-06-28" version="1">MDIILNILAFAQSANNGAAFTFELLYMLFFIVIIAISFIPGLQQQYQMMFMSKDVEKNLNTIENYLKDAKGIAEKLLKEKNFQDPKAFLDRVIDRFVIDPVSIEPTDIISRMKLLLRTNEDTVRQMITKYSPNIDPMSRSQIEVSIEVINALNMIYKVIRHYFLLAKKLKNLMIMYQLQAVAPIYAKLAEAYGKAQKVFLQGIPVGDGLGPLVASRFLMNINDKWSPSKDTIAGSMEFEGRKLIVVKAEGPMATVGEPGEAVQNIVEKEGNKVARIITVDAALKLEGEETGSIAEGMGVAMGDPGPEKIAIERVAAKYNIPIDAVIVKMSMEEAITEMRKEVYEAADKVVNYVKNIILERTKPGDTVVLVGVGNTVGIAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T3</accession>
    <name>F4B6T3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">Ahos_1749</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T3"/>
    <dbReference type="STRING" id="933801.Ahos_1749"/>
    <dbReference type="KEGG" id="aho:Ahos_1749"/>
    <dbReference type="eggNOG" id="arCOG01452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09634">
        <property type="entry name" value="Cas1_I-II-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050646">
        <property type="entry name" value="Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="284" mass="32606" checksum="FABF1164D39EF130" modified="2011-06-28" version="1">MFVIKKGDNKVEVSPSEVDEILVLSSSLISTKALMVALSHGIDVIFLDSRENPWGILLPSVVTVTVKTKKAQYEAVLNNDTTYGEEIIKCKIFNQHIHLKYWTRSGIKTDYKELIGRDEPTAARIYWQNIALLLPKDIHFDERDADSADQFNMALNYSYAILYNRIFQYLVIAGLDPYLGFVHKDRPGNESLVYDFSEMFKPYIDFILVRAFREGFRLKIKDGLIDRESRSSLARIVVKGMDDKVKEENDHNPKTLVQAIRAHAVKLASSIREKRPYNGFKLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W7</accession>
    <name>F4B6W7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776575.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6W7"/>
    <dbReference type="STRING" id="933801.Ahos_1784"/>
    <dbReference type="GeneID" id="10601284"/>
    <dbReference type="KEGG" id="aho:Ahos_1784"/>
    <dbReference type="eggNOG" id="arCOG01595">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_454649_0_0_2"/>
    <dbReference type="OrthoDB" id="383965at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="575"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="600" mass="68135" checksum="D27BE668C20063EE" modified="2011-06-28" version="1">MNKLALLLIVIVILTQVSEAMVVEYNSTSYSVTIMVSRIFYVPLNDTFAVAVVKFLIYGTVVTIYENGHSVTLYCRNITVMNVTSIKVYPFSNSSSSAIIHVKNVLGENFPCVGIFVYKDYLIVEFSPNHENFRFYVFNCRLNKINEFEFHNTACKGTLGISGKYLLYYSGKINYKNITIIGCRIKNFLRSFLFLPIYPFKNSTVTRLTIPVLSNITVFMYNMLTGNIEKEIYLKNVSIYEYPSLILIHNVTIMWYPLFVSVRMNDFIYNNKILDLETICYGNSSVNNVSFYTINLCNGKISLSPKFNICSSIMCGDNLIAVILPNQYFGIFINPIETVELYTSNFTFLGKVTFSPPFIISNPPPSHSQGIVTFSPPLYILLIPEKTEVYSPYDTIANFVYYDGYIYLNNCNCILILHNNQSIQFQNAQLFFLCKPIVVEKEAKGYTVMELNGDKILKCLHINAEYFSTQNSIIFCKIETIYKNSTYKINISTNIYFPGVGNFIIFHEGNSSIIVTLPNLNVINLSSPYIIGVYDNSPVLVNVENLMLNCKCCSAFSNITFGKFLDCNTADIRGYFLLLIISILVLVTIVGAVTLKKLMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B730</accession>
    <name>F4B730_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Flap endonuclease 1</fullName>
            <shortName evidence="10">FEN-1</shortName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fen</name>
        <name evidence="14" type="ordered locus">Ahos_0625</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775429.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B730"/>
    <dbReference type="STRING" id="933801.Ahos_0625"/>
    <dbReference type="GeneID" id="10600072"/>
    <dbReference type="KEGG" id="aho:Ahos_0625"/>
    <dbReference type="eggNOG" id="arCOG04050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09903">
        <property type="entry name" value="H3TH_FEN1-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="12">
        <location>
            <begin position="135"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="10">
        <location>
            <begin position="336"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="82"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="344" mass="38849" checksum="2708A3F73774AE8A" modified="2011-06-28" version="1">MVKEIKREVQLSELKGKKVSIDAYNAIYQFLTAIRQPDGTPLMDSQGRVTSHLSGIFYRTISLLEEGVIPIYVFDGKPPELKAQELERRRKIKEEAEKKLEKAKEEGETKELKKYSQMATRLTNDMAEEGKRLLEAMGIPVVQAPSEGEAEAAYLCSQGYTWAAASQDYDSLLFGANKLIRNLTLTGKRKLPKKDVYVEIKPELIELEDLLKKFGITREQLVDIGILIGTDYDPDGIKGIGPVTALRIIKKYGKIEKAVEKGELPKYILDLNINEIRSIFLNPPVVKPESSLDLKEPNEEEIKKILIDEHNFSEDRVTNGIERLIKAGKEAKGASRQSGLDQWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B734</accession>
    <name>F4B734_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small zinc finger protein HVO-2753-like zinc-binding pocket domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93517.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="65" mass="7307" checksum="4DAB2D47DC419B7A" modified="2011-06-28" version="1">MSVKLSLREEIEPPVCSSCGRIIHPRERGVAFYCPNCGEVLILRDKYCRLQGVEYTCPKCGFTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B756</accession>
    <name>F4B756_ACIHW</name>
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        <recommendedName>
            <fullName evidence="11">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="11">IPP isomerase</shortName>
            <ecNumber evidence="11">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Type 2 isopentenyl diphosphate isomerase</fullName>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fni</name>
        <name evidence="13" type="ordered locus">Ahos_0652</name>
    </gene>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10600103"/>
    <dbReference type="KEGG" id="aho:Ahos_0652"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="371955at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
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    </dbReference>
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        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 11" id="KW-0288">FMN</keyword>
    <keyword evidence="9 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN-dependent dehydrogenase" evidence="12">
        <location>
            <begin position="175"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="5"/>
            <end position="6"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>FMN</name>
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        </ligand>
    </feature>
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        <ligand>
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        </ligand>
    </feature>
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            <end position="95"/>
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            <name>substrate</name>
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        </ligand>
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        <location>
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        <ligand>
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            <position position="158"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <location>
            <position position="190"/>
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        <ligand>
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        <location>
            <position position="215"/>
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        <ligand>
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        </ligand>
    </feature>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93539.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="365" mass="40161" checksum="2774F4209F9786ED" modified="2011-06-28" version="1">MITNRKLEHVEICLYEDIEGYIPTLFDDVVLIHQAMPCLSFNEINTNVKFLNKEISAPLMVTGMTGGNEALGKINATIAEVIEELRLAMGVGSQRIAIERADARESFKIVRKKAPTSPIIANIGAPQLAKGYGLKELKEAVSMIEADAIAVHLNPAQELFQPEGEPEYPSDILIKLRDISKELGVPIIIKETGTGISMETATKFKEIGIKYFDVSGQGGTSWIAVEMVRDKRKNNWKKESAELFAGWGIPTAASIIETRFAVPDAFIIGSGGIRNGLQIAKAIALGADIAGMASPVLKKAVEGKESLVSFFNKVIFELKAAMMLTGAKNVEELKRVPVVIWKELREWMESRGISLSTYEKLRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B8</accession>
    <name>F4B7B8_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1865</name>
    </gene>
    <organism evidence="2 3">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052528">
        <property type="entry name" value="Sugar_transport-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526">
        <property type="entry name" value="INTEGRAL MEMBRANE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23526:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="381" mass="42523" checksum="04EE5DA91A890C7F" modified="2011-06-28" version="1">MILHYVMKPLRTYVMIRNFALNLSQPFISFVSAASGISGENLAIVSSAGSALPSFIQFVMGFFNLRGKIITFYGTLAAGLLWISLSFIPFGVFFVVIYVLLEASLGISAYGWYLIMERISNTSRGKILAQYSFYSVIGGMIATLLTGFVVNKDFSEVRYFFFFSGSLFLLSSLISLKFDVDYSKEPLKGLSLNKELSKFLIITFLFDVVWSMAWPLFPLAQVYLFDMNFENIAIINLIANFSTLLLQRKIGELVDKNRKLMMFLGRLALSVFPLAYALSTSVYEIYLANVVAGFTSSINSTAYLSYIYDNSKDVRKGIGLYNAVSGLGDLIGSSVGMLGIEELEFLGMVIAIKLMMLIIASLRMVASFVYLKIQEVKPVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J6</accession>
    <name>F4B7J6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps6e</name>
        <name evidence="5" type="ordered locus">Ahos_0714</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J6"/>
    <dbReference type="STRING" id="933801.Ahos_0714"/>
    <dbReference type="KEGG" id="aho:Ahos_0714"/>
    <dbReference type="eggNOG" id="arCOG01946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275302_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003292">
        <property type="entry name" value="PRK04290.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="194" mass="21526" checksum="712E2676BCEF1EA0" modified="2011-06-28" version="1">MKVKVKADENVKSIDGEKDGKALPIAKLNSKLKQALNLDKLLTLQIEKQEGDKKVKIKVHFLVQVDDSLPDNEVHISKNIAEKFGAEDFEALAYRTKAFQISIDQSKLNLFGTKIGDKINLVISDTPFTLKITGGSDNTGFAMRPDISGPAKRRVLVSGPPGYIPREDGERKRKMLRGDTISNEIVQINTVIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L2</accession>
    <name>F4B7L2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase Rpo13 subunit HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775533.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L2"/>
    <dbReference type="STRING" id="933801.Ahos_0730"/>
    <dbReference type="GeneID" id="10600184"/>
    <dbReference type="KEGG" id="aho:Ahos_0730"/>
    <dbReference type="eggNOG" id="arCOG05938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177471_0_0_2"/>
    <dbReference type="OrthoDB" id="44217at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021985">
        <property type="entry name" value="RNA_pol_Rpo13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12136">
        <property type="entry name" value="RNA_pol_Rpo13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase Rpo13 subunit HTH" evidence="2">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="97" mass="11553" checksum="15DB26E2F9536E6A" modified="2011-06-28" version="1">MSDDYENKEEEEPKEEKEEKTEEEEEELPALTLQDIELLMKNTEVWDELIAGKITIEQAKKLFEENYEELANSQKKKTARKKTTKSKKTKVKEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M4</accession>
    <name>F4B7M4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plasmid stabilization system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776686.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M4"/>
    <dbReference type="GeneID" id="10601397"/>
    <dbReference type="KEGG" id="aho:Ahos_1898"/>
    <dbReference type="eggNOG" id="arCOG01665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157019_0_0_2"/>
    <dbReference type="OrthoDB" id="38061at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12834" checksum="D3B92A6EED239480" modified="2011-06-28" version="1">MICEKWELKFLFRKRITNLEELSEFLAKEFPHEEVVMLIFDKLYLLREDPKKYAREKLKNQTDKDGRPLFSIEVTGDIRIVYSFEPKNCTVFIWRIGKHKKAYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P1</accession>
    <name>F4B7P1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1915</name>
    </gene>
    <organism evidence="1 2">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AEE94788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <sequence length="99" mass="11633" checksum="ADE40D225FC31473" modified="2011-06-28" version="1">MHSWYVVLCSSIESYYSSKSSKSSYMVPDTCMCNSKLSANVTRLVFCELNCHGRKVCVWIFNFTSPVYSPKLVEHVEKYRILRARARKLLILTDIKERW</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Wang S.Y."/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="AEE94806.1"/>
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    </evidence>
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    </evidence>
    <sequence length="124" mass="14015" checksum="3605B88BDA4B98EF" modified="2011-06-28" version="1">MRSMERKVKFPDSREVDVLTIFKFVYGLSNSELEIIKLLVNTKGKLSAEDIASSLNISRNTVTKPVNLLLSKGLVLRAKEKGKTTGRPRLVYFATQDIYSKLLTDLQSIVNNSLKEISKIRKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26364" checksum="477A974478820A7E" modified="2011-06-28" version="1">MYRILGYDMQHVRVYLNEGEMIYGDGGHLVAKSPSVKINYAAQGGIIKSFERELTGSRFFVMQVYGPGVAEFSSFLPGRIVKIDLNGNGIRVEHNSFLFAEQGVQYSATLDKISVGWLGGEGLLMAHFSGQGSVFVHALGGVSSFVLQQGEEIEVEEGHLLAFDDSMQYSITRAGGIKTMLFGHIEKEGAFFIKLTGPGRVWLHNVSLEQLIAKLNLMVGGNGGPAQPQDPSIDIQNLRIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V2</accession>
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        <recommendedName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B7V2"/>
    <dbReference type="STRING" id="933801.Ahos_0743"/>
    <dbReference type="GeneID" id="10600200"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00164">
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        <property type="match status" value="1"/>
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    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="146" mass="16148" checksum="D079086CC21B3B4C" modified="2011-06-28" version="1">MSGKSPKGLYAARKLKLKRLKFRWSQRSFKARMLQLKKRFDPLEGAPMARGIVLEKVGIESRQPNSAVRKCVRVQLVKNGRVVTAFVPGDGGVNFIDEHDEVVIAGIGGTLGRSMGDLPGVRYKVIMVNGVSLDALYKGKKQKPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V9</accession>
    <name>F4B7V9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Malate dehydrogenase (Oxaloacetate-decarboxylating) (NADP(+))</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0750</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775552.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V9"/>
    <dbReference type="STRING" id="933801.Ahos_0750"/>
    <dbReference type="GeneID" id="10600207"/>
    <dbReference type="KEGG" id="aho:Ahos_0750"/>
    <dbReference type="eggNOG" id="arCOG00853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034446_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004470">
        <property type="term" value="F:malic enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051674">
        <property type="entry name" value="Malate_Decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43237:SF4">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="4">
        <location>
            <begin position="15"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="3">
        <location>
            <begin position="160"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="425" mass="46583" checksum="ED7EF860360C3BA9" modified="2011-06-28" version="1">MDYDKLAIEVSSKYKGKIQILPKVPIRSLEDFAILYTPGVAAVSKEISKNPDLSFQYTYRWNSIAVVTDGTRVLGLGNIGPEAAMPVMEGKALLFKYLGGVDAIPLPIGTKDPDKIIETVKILEPAFGGINLEDIESPKCFYILDKLREELHIPVWHDDQQGTAGATLAGLISALEIVGKSPTKIKIVLYGVGAANIATARLLKSYGIPYSNMILIDTKGPLYRGREDEEEIKNTNPWKYELLKESNKDNVTTVEKAFEGADVLIAASKPGPHTIDPKLISKMNKDAIVFALANPVPEIWPDEAKKAGARIVGTGRSDLPNQINNSLIFPAVFRGALDVRAKTITDDMVIAAARELSAFAREKGLSEDYIIPKMTEWEVYPRVAAAVGSKAVQQGIARVNLSYEEIYNNARQMIENARKLLASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y7</accession>
    <name>F4B7Y7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytosolic acyl-CoA thioester hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0778</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775580.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y7"/>
    <dbReference type="STRING" id="933801.Ahos_0778"/>
    <dbReference type="GeneID" id="10600237"/>
    <dbReference type="KEGG" id="aho:Ahos_0778"/>
    <dbReference type="eggNOG" id="arCOG00773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073524_0_0_2"/>
    <dbReference type="OrthoDB" id="15030at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="2">
        <location>
            <begin position="149"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="305" mass="34545" checksum="DB6863198313B513" modified="2011-06-28" version="1">MRTETYYNVFPWHTNHFGSLHGGIYMSWLIDTSGILMSSISKGNYLLASVDYIFLFRPARLGDVLRVIAKANATWNSSVEIEVKGCIRREDKEELGAVGLMTYVAVDENNKPRKLDIKIPPDENAEKRRMQRLERKKHTMNDIDDILDLSFTRSYIRTIYPEHGFGNGILYAGKMYTMLDEALAIVAKLYSKGNAFTVSAGPADFISPVKIGDILEIQGAVEYTGNTSLDVGGKVYAINHYTGEKRLVTSTVFSFVAIDENGKPRPIPKLNPDSEKEKKIFEERLKEREERVKTSKRLQSEISCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B801</accession>
    <name>F4B801_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps17e</name>
        <name evidence="5" type="ordered locus">Ahos_0792</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775594.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B801"/>
    <dbReference type="STRING" id="933801.Ahos_0792"/>
    <dbReference type="GeneID" id="10600253"/>
    <dbReference type="KEGG" id="aho:Ahos_0792"/>
    <dbReference type="eggNOG" id="arCOG01885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176720_0_0_2"/>
    <dbReference type="OrthoDB" id="52479at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
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        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="77" mass="9080" checksum="B1C697322252E5C7" modified="2011-06-28" version="1">MGNIYTRDIKRIAAEIYEKYKDEVTTDYSKNKEVVKKYIDVYSKKVRNRIAGYLTRYAKRMSSQKSVSEGEVVEESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B811</accession>
    <name>F4B811_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sm-like ribonucleoprotein core</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B811"/>
    <dbReference type="STRING" id="933801.Ahos_0802"/>
    <dbReference type="KEGG" id="aho:Ahos_0802"/>
    <dbReference type="eggNOG" id="arCOG00999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145829_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.60">
        <property type="entry name" value="Like-Sm ribonucleoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028277">
        <property type="entry name" value="Lsm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037156">
        <property type="entry name" value="Lsm_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14894">
        <property type="entry name" value="Lsm_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="10"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="150" mass="16913" checksum="44EE19239A495D6D" modified="2011-06-28" version="1">MLTVMSISRRVVYDINSLIDKTVIIKLTNGKTYTGQLSSFEIDPFMVSISNAKDNENNVYYKAIINGSIISEILIKNAPIFDVKEFASLIEKSLNLRPGDIKVYEEAGVITVLEKIKVTENGVEGSGPLAQRIYDLFNEYVEKKKRETSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B848</accession>
    <name>F4B848_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2000</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776787.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B848"/>
    <dbReference type="STRING" id="933801.Ahos_2000"/>
    <dbReference type="GeneID" id="10601499"/>
    <dbReference type="KEGG" id="aho:Ahos_2000"/>
    <dbReference type="eggNOG" id="arCOG01007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_2_2"/>
    <dbReference type="OrthoDB" id="57034at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="237"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="332" mass="36404" checksum="57F90E725EECBEAC" modified="2011-06-28" version="1">MRKSFFTLFFIVLTIFSFPISFILSLIYGQIYIPFSEIISPHGFYYTILFRIRLPTVIASALIGASLSIAGAIMQLLLRNPLMDPYVSGTASGGAFGAVLAYFLLAFNLPFGWLIYISPLIAFFFSLLATVITLAIGKKTGVYGIIIGGVVVSYLFSSMLTMLLEAIEYKYPQVPPPLFWLLGEIQIVGYEFDEALGILVFLLIIFSIIESRKLDLVHISDELSFAKKVNPNRYRMLWIIFLSIIVGLIVSQVGIIGFIGIIVPHIVRRTIGGNSSTLIPFSAILGSSIMMLSNIIASGALGFVIPVTAITSVLASPIIIYVLVKGRVSEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B849</accession>
    <name>F4B849_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776788.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B849"/>
    <dbReference type="STRING" id="933801.Ahos_2001"/>
    <dbReference type="GeneID" id="10601500"/>
    <dbReference type="KEGG" id="aho:Ahos_2001"/>
    <dbReference type="eggNOG" id="arCOG00198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="215" mass="24209" checksum="67EA57BA5213B368" modified="2011-06-28" version="1">MKLGDREILHGISFSTEGGINVILGPNGSGKTTLLRTIIGMIKPTEGEIQINKRISYVPSEFFPAQMRVKDVLLSGEKKRKIEDYIENAKLLGVEDFLDREFYSLSSGEKRLVLICKALTEGDLVIMDEPLSNLDLANKTRIMNIMKEVSKMKEFLITSHELDIINISNKVIIVKNGEVVYQGKPSDVTEEVLSEVYGVRIRKYEIDGKTIFVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B864</accession>
    <name>F4B864_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776803.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B864"/>
    <dbReference type="STRING" id="933801.Ahos_2016"/>
    <dbReference type="GeneID" id="10601515"/>
    <dbReference type="KEGG" id="aho:Ahos_2016"/>
    <dbReference type="HOGENOM" id="CLU_1471998_0_0_2"/>
    <dbReference type="OrthoDB" id="374138at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="183" mass="21792" checksum="C8DE42AF48F0F80E" modified="2011-06-28" version="1">MILILAENEIPEADYVTYFSKDDISFIFLYYKEGFYRYIGLYKKNKGVVSRVRNIPFYLPFKYVKRLHDNYLLLYLKDDKEKQVIIPAVKVEAYEYLYSQLAYTIEYMMDKLKIEVSPEQGKEIIDILKRGNSIGEDKEGAIYSLKVYRLPETIQISKEVFTDYPLYPPLEVFKEGFEDNNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C6</accession>
    <name>F4B8C6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminopeptidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775641.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C6"/>
    <dbReference type="STRING" id="933801.Ahos_0839"/>
    <dbReference type="GeneID" id="10600307"/>
    <dbReference type="KEGG" id="aho:Ahos_0839"/>
    <dbReference type="eggNOG" id="arCOG02960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_558552_0_0_2"/>
    <dbReference type="OrthoDB" id="34215at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M28" evidence="1">
        <location>
            <begin position="177"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="471" mass="54386" checksum="245CB73DC2B05079" modified="2011-06-28" version="1">MIVERVKELSSLGEIFAGDKIEGKIVNKIRKFFDKCDETKVIPIPVLNYSESHEIFGKNKIENSEYLPYSPSADIEGKLTYSLDECKDSILGVKIENLFDIPRYYIKAEERDCVAIVFFTDKRRKFVIKSDPLLNLFPTSPPKIPGIIIPENEKNKIEDKLSIKASVTIKESTGYIIECIKNSKNDKKVYITTHHDHWFKGEHDNLLGVSLLPELKSEKYELHLISFTAEEAGALGYQSFSWSYGSRKYFELQKKGLNKDIILNINLDNIDPCNLKIKAVPSISSVFEKYFNNSIVYEPEIYSDGYTFIKNGIPSVTLEGYYREYHSIDDEIIPSEEVCISSLILSINKILNDENIEQIRYDMLKSELMQFMNITQAPMRTYLLNILDNLNNKEIYENLLKLYGGILPRDGLAIVKLFHKLAGIKYEKPVYIEGKPALKISSNDKLYLHSIAKEFEDEYMNRLYEISKKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F0</accession>
    <name>F4B8F0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl18</name>
        <name evidence="7" type="ordered locus">Ahos_0863</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775665.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F0"/>
    <dbReference type="STRING" id="933801.Ahos_0863"/>
    <dbReference type="GeneID" id="10600331"/>
    <dbReference type="KEGG" id="aho:Ahos_0863"/>
    <dbReference type="eggNOG" id="arCOG04088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_2_0_2"/>
    <dbReference type="OrthoDB" id="8644at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057267">
        <property type="entry name" value="Rbsml_uL18_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006342">
        <property type="entry name" value="PRK08569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="21984" checksum="2C5569F61B559F53" modified="2011-06-28" version="1">MALGPNYKVKPRRRREGKTNYYKRYVYVLSRAIRLVVRLTNEYVIVSVMKFDPKGDITIASAHSIELVKKYGWKGDTNNTPAAYLTGFLAGLRAKKAGIESAVADIGLFTPTKGARVFYAIKGALDAGLKVPMGDIELDENRTKGVHISEYAKKLEAENPEKFNKLFSRYLSRGLNPKDLPSHFEEVLNKIKNSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G3</accession>
    <name>F4B8G3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775678.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G3"/>
    <dbReference type="STRING" id="933801.Ahos_0876"/>
    <dbReference type="GeneID" id="10600347"/>
    <dbReference type="KEGG" id="aho:Ahos_0876"/>
    <dbReference type="eggNOG" id="arCOG08307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271087_0_0_2"/>
    <dbReference type="OrthoDB" id="38163at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="103" mass="11712" checksum="6BEEBCFF813C5C77" modified="2011-06-28" version="1">MPSIKVIFGSYGDNGVTSLLYEVIQSLPNKYDIKIDVETDLLYDYSSPMIEIDGRRILFDYIDENDAKVKLSKLIKGELIEDHKPFKIFYSQNGIFGNGVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L5</accession>
    <name>F4B8L5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2096</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776882.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L5"/>
    <dbReference type="STRING" id="933801.Ahos_2096"/>
    <dbReference type="GeneID" id="10601596"/>
    <dbReference type="KEGG" id="aho:Ahos_2096"/>
    <dbReference type="eggNOG" id="arCOG01482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_2_0_2"/>
    <dbReference type="OrthoDB" id="115072at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000030">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase, carboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="8">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="7">
        <location>
            <begin position="105"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="279" mass="30915" checksum="9A549F9CB293197A" modified="2011-06-28" version="1">MIEQIFISKLMQFLEEDYYPEDLTSRIVEGYKIRSYVRSKDEGVLAGNKFIIPFLNYLGFNVKSSMTDGVEVKKGDNLLVFEGEADKSLSVERLVLNLLGKLSGIATATYTMVKLAREVNPTVRIAGTRKTTPGLRVFEKYAIEVGGGDPHRFNLSDAVLIKDNHLAIIGDIEKAVKMARQIASFTKKIEIEVSSYEDAIKAYRAGVDAILLDNMSVNEIEEVVSELKGKVLLEASGRITPENVKSYAKTGVDIISSGYITHSSRSLDFSMDVELTSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N4</accession>
    <name>F4B8N4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA 3'-5' helicase Hel308</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hel308</name>
        <name evidence="13" type="ordered locus">Ahos_2115</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776901.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N4"/>
    <dbReference type="STRING" id="933801.Ahos_2115"/>
    <dbReference type="GeneID" id="10601615"/>
    <dbReference type="KEGG" id="aho:Ahos_2115"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006553_3_0_2"/>
    <dbReference type="OrthoDB" id="371946at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18028">
        <property type="entry name" value="DEXHc_archSki2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048772">
        <property type="entry name" value="Hel308-like_dom4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053416">
        <property type="entry name" value="Hel308_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040935">
        <property type="entry name" value="helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF10">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HEL308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21280">
        <property type="entry name" value="Helicase_dom4_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="36"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="233"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="704" mass="79410" checksum="B4BA49E8D10C9106" modified="2011-06-28" version="1">MEFTPIDKLNLDERIIKILKKKGIEKLNPVQTDAVNSGLLEGKRLLVTSPTGSGKTLIAELGIISHLLSNGGKAVYITPLKALTAEKYNELKDWEELGFKVGMTSGDYDSDDAWLKNYDIIVSTYEKIDSLWRHNPSWLSEVDYFVLDEFHYLNDEERGPVVESVAIRAKRKNLLALSATISNYEKIANWLNAIPVTTNWRPVPLREGVIVEGKKSYQVIFPDETIEIKGNDPIIAYTLYVLEHGGQVLVFRNSRKMAESTAKKISSSMGLLSLPDKELLKVGEEIKNVEDAGSDEKEELYELVVRGVAFHHAGLSKGLREIIERAFRERKIKVIVATPTLAAGVNLPARAVIIGDFHRFNRKILGYQEEIPIMEYKQMAGRAGRPGFDKEGEAVIVVRNKKEAEKVFKKYILSPPEPIESRLGNESAFYSFLLGIISSEKKISEDDLEDYAMDTLLDKDVAEKYVKRGITWLRDNEFIAGDDELVLTKFGKRVADLYINPFTAKVFKDYLTKSDKSCNIAYLHLVAYTPDGPTVSVTRSELEELVDEAPCPLFIEEPEDEDEFYNYVSALKVAMIINDWIEEVDEDVILGRYGIGSGDLRSIIDTMDWLTYSGYNVAKVLDLKEHYDVLYTLNLRVKDGVKEELLDLVKIQGVGRKRARILYNHGIKKPEDVVMNVDKVKSLLGQKIGEKIAKEAARLIAGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8P0</accession>
    <name>F4B8P0_ACIHW</name>
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        <submittedName>
            <fullName evidence="5">ABC transporter, NrtD, SsuB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8P0"/>
    <dbReference type="STRING" id="933801.Ahos_2121"/>
    <dbReference type="KEGG" id="aho:Ahos_2121"/>
    <dbReference type="eggNOG" id="arCOG00193">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03293">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050166">
        <property type="entry name" value="ABC_transporter_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788:SF13">
        <property type="entry name" value="ALIPHATIC SULFONATES IMPORT ATP-BINDING PROTEIN SSUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788">
        <property type="entry name" value="TAURINE IMPORT ATP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="15"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="273" mass="30708" checksum="AD0589544705362E" modified="2011-06-28" version="1">MRLSHLGGNTVGRILEGIDLGYTYPNGYKVFDHVNIETYDNELIAIIGPSGIGKSTLLRILGGFVKPDHGEVRLLGKKITQPTPKIALIHQSIVTFPWFTALENVKLGLKYKKLPKEKEDEIARKMLELVGLSGFEDFYPKQMSGGMRQRVAIARALAADPVVLLMDEPFAHLDELTAEGLRREIYSILFNEDTSLRSAVLVSHNLNEVVELADRVYILNGRPASVVGEVKIELDRPRKPKDDRFQAYLDQLYSLLTPVEKNVEQKDRGKQDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U3</accession>
    <name>F4B8U3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0927</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775729.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U3"/>
    <dbReference type="GeneID" id="10600399"/>
    <dbReference type="KEGG" id="aho:Ahos_0927"/>
    <dbReference type="eggNOG" id="arCOG07290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500309_0_0_2"/>
    <dbReference type="OrthoDB" id="34684at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="187" mass="21444" checksum="8FAFB9B0CEF33701" modified="2011-06-28" version="1">MKLFGYNVDSLLTPEVMYIVTRRIFVPFLAEEFPEIISLDTNLNIIRELIIIKDHLLKGNKIQIGYKYTVNSKNDGKLSSLNDCKRIVFGINAKLLKSLNALSTDFIFFGFKNELEKILILHDIPIIAKDKDELYREITKYLASWGINISSIPAEISNMPKNKINVKSKIIDIDYALFSSQFSSNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X2</accession>
    <name>F4B8X2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775758.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8X2"/>
    <dbReference type="STRING" id="933801.Ahos_0956"/>
    <dbReference type="GeneID" id="10600428"/>
    <dbReference type="KEGG" id="aho:Ahos_0956"/>
    <dbReference type="eggNOG" id="arCOG05922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867721_0_0_2"/>
    <dbReference type="OrthoDB" id="33875at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="327" mass="37707" checksum="9563478BE0ED8E0D" modified="2011-06-28" version="1">MSLFKKEEKKIFHIDRLPEEMKVAIKTLIDSSIPDVAKAYGFDYLYPKLGEPIFIPYGRLDGKYKSTIDAFEKILEEVEELKDNLKEYSKWYGNVKLFDHYRFTFYSYVDPNEGMKVGIGADPLSSPNSLFDVQSLCSALDKGKSIIILNPALSGYISSTCLSSFNIKFVDTISKRKDEIIDAYTWLNKSFHENFDKDKVYDVELGREYMDRLFNVILSEVRNYSTESKEGDIAILPLLSYEEYGEKESIKGVLQNDEKYKKYIDEGRLDEISLIPILFSGSLKELNEIKDKYSKVIVISDKKIRIKIDGNVKQLNDKILVIENAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B912</accession>
    <name>F4B912_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Single-strand DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2164</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776950.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B912"/>
    <dbReference type="STRING" id="933801.Ahos_2164"/>
    <dbReference type="GeneID" id="10601667"/>
    <dbReference type="KEGG" id="aho:Ahos_2164"/>
    <dbReference type="eggNOG" id="arCOG01510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110881_2_0_2"/>
    <dbReference type="OrthoDB" id="6262at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="3">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="142" mass="15940" checksum="3C529BF501D478CC" modified="2011-06-28" version="1">MEEKIGNLKGGMENVNVTGRVLQVGEQKVVQTRNGQRTIREVMIGDETGRIKLTLWGNQGDNIKEGQVIKVENGWTTVFKGQVQLNAGSRSKISESQEEVPEADQIPENTPTDNSPPRRNFRGGRRNFRGRRPPQRGGEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B972</accession>
    <name>F4B972_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Threonyl/alanyl tRNA synthetase SAD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0979</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775781.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B972"/>
    <dbReference type="STRING" id="933801.Ahos_0979"/>
    <dbReference type="GeneID" id="10600451"/>
    <dbReference type="KEGG" id="aho:Ahos_0979"/>
    <dbReference type="eggNOG" id="arCOG01254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_3_2_2"/>
    <dbReference type="OrthoDB" id="11392at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000010">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053424">
        <property type="entry name" value="Alanyl-tRNA_Edit-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040865">
        <property type="entry name" value="a_tRNA_ed_AlaXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="235" mass="26397" checksum="AFD1BDA82FD4E56E" modified="2011-06-28" version="1">MATEELYLKDSYIKEFEAKVTKVNGNEVILDKTAFYPGGGGLENDVGKLIKNNGETINVKEVKRQGDEIAHVIEGGELREGDNVKGVIDWDRRYKMMRLHTASHVMAAVAYSQFNALITGGHISPEYAKDDFNLENKDLIPQIVEKANEILSKGIEVKVYFLPREEALKIPGIVKLAERNPPSIPIWRIVEIPGIDIQADGGPHVKNTSEVGKIRIIKIENRGKNRKRVYYTVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B984</accession>
    <name>F4B984_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metal-dependent carboxypeptidase</fullName>
            <ecNumber evidence="1">3.4.17.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0991</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of a C-terminal amino acid with broad specificity, except for -Pro.</text>
            <dbReference type="EC" id="3.4.17.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.19" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775793.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B984"/>
    <dbReference type="STRING" id="933801.Ahos_0991"/>
    <dbReference type="GeneID" id="10600464"/>
    <dbReference type="KEGG" id="aho:Ahos_0991"/>
    <dbReference type="eggNOG" id="arCOG04247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032916_1_1_2"/>
    <dbReference type="OrthoDB" id="7244at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06460">
        <property type="entry name" value="M32_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001333">
        <property type="entry name" value="Peptidase_M32_Taq"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217:SF1">
        <property type="entry name" value="CARBOXYPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34217">
        <property type="entry name" value="METAL-DEPENDENT CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02074">
        <property type="entry name" value="Peptidase_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006615">
        <property type="entry name" value="Zn_crbxpep_Taq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00998">
        <property type="entry name" value="CRBOXYPTASET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52034">
        <property type="entry name" value="PEPTIDASE_M32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR006615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="482" mass="56377" checksum="8C58E2C6A1487A38" modified="2011-06-28" version="1">MLEDILEEYKKAWALGHALALMGWDLETYMPQEGSKYRGEAIAQLITLRRNEIMKLAEKVDRIDESKLDDKERGIIRVLRREIKYYRAVPKEVDEELARLTSEAFIAWSKAKNESNFSIFKPYLEKIVDLKRKIAESLGYEKHPYNALLDLYEEGFTVDDADKVFSSLLPELKTILEKILSSGYYPTHHPLEDMTYDVEVMKKVNDEIINILEMPRSKFRIDISPHPFTERISTDDVRITTRYEGKDFRATMFSVIHESGHAIYELQIDPSLEMTPVGQGVSMGVHESQSRFWENTIGRSREFVGIIYPLLKRFLDLKYDEEEIYKYFNVVRPSLIRVDADEVTYNLHIAIRYEIEKKLIAGELKVDEIPPLWNDLSEKYLGIRPKNDAEGALQDVHWSGGSFGYFPTYTIGNIIAGMIYAKFNVLGKVRERKFNEIKEYLREKIHKYGAIYPPKVLLTRSFGDAYNPEYFIKYLREKYLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A2</accession>
    <name>F4B9A2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A2"/>
    <dbReference type="STRING" id="933801.Ahos_1009"/>
    <dbReference type="KEGG" id="aho:Ahos_1009"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998621_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="124" mass="14522" checksum="653D9D00588F8A52" modified="2011-06-28" version="1">MDKYSLRDYYLWKLQGDLVYVLDIDGNIAGVIDIVENNDSLLIDMLARNILVKAEKVGSRLLDFAEYLAKIKGKKLIKLEALDTAIEFYKKMGYKEVYKRFDKEWGLLTVMEKKIEKYVIKPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C2</accession>
    <name>F4B9C2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13 14">Malto-oligosyltrehalose trehalohydrolase</fullName>
            <shortName evidence="14">MTHase</shortName>
            <ecNumber evidence="4 13 14">3.2.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 14">4-alpha-D-((1-&gt;4)-alpha-D-glucano)trehalose trehalohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 14">Maltooligosyl trehalose trehalohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Ahos_1029</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkage in 4-alpha-D-[(1-&gt;4)-alpha-D-glucanosyl]n trehalose to yield trehalose and (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="3.2.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.141" evidence="4 13 14"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775831.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C2"/>
    <dbReference type="STRING" id="933801.Ahos_1029"/>
    <dbReference type="GeneID" id="10600504"/>
    <dbReference type="KEGG" id="aho:Ahos_1029"/>
    <dbReference type="eggNOG" id="arCOG02951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020726_2_0_2"/>
    <dbReference type="OrthoDB" id="18347at2157"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033942">
        <property type="term" value="F:4-alpha-D-(1-&gt;4)-alpha-D-glucanotrehalose trehalohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11325">
        <property type="entry name" value="AmyAc_GTHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02853">
        <property type="entry name" value="E_set_MTHase_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.760">
        <property type="entry name" value="E-set domains of sugar-utilizing enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012768">
        <property type="entry name" value="Trehalose_TreZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044901">
        <property type="entry name" value="Trehalose_TreZ_E-set_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02402">
        <property type="entry name" value="trehalose_TreZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF11">
        <property type="entry name" value="MALTO-OLIGOSYLTREHALOSE TREHALOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006337">
        <property type="entry name" value="Trehalose_TreZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="18">
        <location>
            <begin position="88"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="252"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="312"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="380"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR02402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR006337-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006337-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR006337-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEE93915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="559" mass="64137" checksum="5B73FCDD07C3449B" modified="2011-06-28" version="1">MDIGANFDGKSTFFRVWAPYNKEVFLVLYKDGERKVIKMELEDEERGYFSVEVDNAKPGDMYTYMIGGKEYPDPASRFQPEGVHGKSQIIGDFSWDDYNWKGIDFPIIYELHVGTFGGDFEGVKRKLDYLKELGVTAIELMPVHQFAGNRNWGYDGVLLYAVQNSYGGPQKLKELVNEAHKKGLGVILDVVYNHVGPEGNYLSVFGPYFSTRYKTPWGPIFNFDEAYSDEVRHYVVQNAVYWIKEYHVDGLRLDAVHSIFDNSPKHILEEIRDAVKEANPKALLIAESDLNDPRIILPKDKCGYCIDAQWSDDFHHSLHALLTGERDSYYADFGEITQLAKAIKDVFVYDGVYSKFRKKTHGRQVGNLPGNKFVVYAQNHDQVGNRKDGKRLISLVGEKALIAPFLYILSPYIPMIFMGEEYGETNPFLFFTDFSDPEIIKGLREGRKRELGEYYYDPQDYSTFEKSKLSWKLDERILSLYKELIRLRKELFNDFSRNVDVKIEGNACIIIRRKEKYLVIACFSKTHLDLKAKSIITTVGMPSSLPGEVSVGAGVYEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C6</accession>
    <name>F4B9C6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775835.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C6"/>
    <dbReference type="GeneID" id="10600508"/>
    <dbReference type="KEGG" id="aho:Ahos_1033"/>
    <dbReference type="HOGENOM" id="CLU_2190888_0_0_2"/>
    <dbReference type="OrthoDB" id="372933at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="108" mass="12799" checksum="40A46F91A5C54C83" modified="2011-06-28" version="1">MQLNLAEVVSNIFPITRDEIERIYINKNKFIVVIYDFSTSKSRKYEGELKRNKIIFWRNKIKLQVPLKGVRLLRKPIEVGKLDEFEIWEIKGDEKLPSFPLEMSVISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D2</accession>
    <name>F4B9D2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">L-sulfolactate dehydrogenase / malate dehydrogenase (NAD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775841.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D2"/>
    <dbReference type="STRING" id="933801.Ahos_1039"/>
    <dbReference type="GeneID" id="10600514"/>
    <dbReference type="KEGG" id="aho:Ahos_1039"/>
    <dbReference type="eggNOG" id="arCOG04874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040452_3_1_2"/>
    <dbReference type="OrthoDB" id="40552at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="entry name" value="Hypothetical oxidoreductase yiak, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091:SF0">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="L-sulfolactate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="320" mass="35124" checksum="B0BD9EE78F5995C0" modified="2011-06-28" version="1">MKIPVNELKEIIIHILDKRGIEDSSVIAEHFIEAELRGHSSHGVQRVIPLVKGIELGTIERKLNYKILKSTSSSLLIDARRSIGIVLWNKIINELEFKEPISIVAVRNASHIGFLGYYTRKLAEKGYSAIMFGNAEPAVVFPGKAEKLLSTTPLSISIPSNPPVVLDMALSLTARGKIIEAQRKGEKIPYGVAVNERGEITTDPTEALKGGLLPLGGVKGFYLMLTLELLTSFLTGSAVGPEVKGVLNTENPPNKGEVLIVINPKFTEEENHGIDLMRRILGSLPGDRGDKLLKNSGNEINIDEKLFNTLNEMAKKIPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F9</accession>
    <name>F4B9F9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">D-arabinose dehydrogenase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2234</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777020.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F9"/>
    <dbReference type="STRING" id="933801.Ahos_2234"/>
    <dbReference type="GeneID" id="10601739"/>
    <dbReference type="KEGG" id="aho:Ahos_2234"/>
    <dbReference type="eggNOG" id="arCOG01457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_2"/>
    <dbReference type="OrthoDB" id="73567at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF4">
        <property type="entry name" value="D-ARABINOSE 1-DEHYDROGENASE (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="10"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="334" mass="37182" checksum="25EDA9A3FD9CFC3D" modified="2011-06-28" version="1">MRAAIFTQIGRPLSIEEIKKPEVKGKEVLLKVLATGLCHGDLHIIFGEWKDDIPVNPPRILGHEIVGEITEDTEHFKKGDKVLVYNAFGCGTCKYCKRGYPQFCEKVKILGVQEDGGFAEYVKVPSEDNLIKIDDNENPIKVAPLADAGVTAYNSVKGIEEDSNVALIGTGAVSLIALQILKSRGIKVTVVGRNPIKLNKALELGADRIIQVKQSYSEDFSARIGREKFDYIVDYIGSDETLRDIVWALDRMGELRIVGEFGGRMEIWEQLLVLRGLRIRGILYGSKEDMINVVKLYKEGKIKTLAVPYKLEEINQAIDDLMSGRIIGRAVIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G0</accession>
    <name>F4B9G0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">RNA 3'-terminal phosphate cyclase</fullName>
            <shortName evidence="6">RNA cyclase</shortName>
            <shortName evidence="6">RNA-3'-phosphate cyclase</shortName>
            <ecNumber evidence="6 7">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rtcA</name>
        <name evidence="10" type="ordered locus">Ahos_2235</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="6 7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777021.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G0"/>
    <dbReference type="STRING" id="933801.Ahos_2235"/>
    <dbReference type="GeneID" id="10601740"/>
    <dbReference type="KEGG" id="aho:Ahos_2235"/>
    <dbReference type="eggNOG" id="arCOG04125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027882_0_0_2"/>
    <dbReference type="OrthoDB" id="7994at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00874">
        <property type="entry name" value="RNA_Cyclase_Class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000002">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="9">
        <location>
            <begin position="179"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="6">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE95106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="335" mass="36977" checksum="16F58B3C78634AEA" modified="2011-06-28" version="1">MIEIDGSFGEGGGEILRTSLTLSALTGKPFRIYNIRAKRKNPGLQRQHLSAVRLVKELCNAESKGDYLGSQELVFIPHEIKNEGDFTLDVTTAGSVTLIAVSVIPLIINRNIKIRLIGGTDVPKSPTIDYMRLVYLEILKKIGIQGEIKLIKRGHYPRGGGIIELSNFKGKGEEFEIVEMGKIEEIKGISHVSSLPSHIAERQAKSAEEFIKKFLSVNVNISLDIRQGESEGTGITLAAYGKSIMGSDSLGEKGKRAEKVGEEAASKLIEDLKTNAGVDRHMSDMLMLYASLYKGKFTGAMLTMHARTNTEIIRKFIQDRKIEVIEGKPFTFKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L8</accession>
    <name>F4B9L8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775850.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L8"/>
    <dbReference type="STRING" id="933801.Ahos_1050"/>
    <dbReference type="GeneID" id="10600524"/>
    <dbReference type="KEGG" id="aho:Ahos_1050"/>
    <dbReference type="eggNOG" id="arCOG07177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173009_0_0_2"/>
    <dbReference type="OrthoDB" id="39794at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="85" mass="9506" checksum="FA3DED7F298533F4" modified="2011-06-28" version="1">MAKDVLGILGDYEPKGKMIQGVGKFILWGLDDPFHITTISGSVIYGVGKIIESRSPYGLRRAKMDLAEIKRQMRELILDISSLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M7</accession>
    <name>F4B9M7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">X-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775859.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M7"/>
    <dbReference type="STRING" id="933801.Ahos_1059"/>
    <dbReference type="GeneID" id="10600533"/>
    <dbReference type="KEGG" id="aho:Ahos_1059"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_0_2"/>
    <dbReference type="OrthoDB" id="1346at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01092">
        <property type="entry name" value="APP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF9">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="4">
        <location>
            <begin position="135"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="352" mass="40147" checksum="007C19196E7A52AB" modified="2011-06-28" version="1">MDRIGKLQKMMRDRGIDYVIIGPTSNMFYLTNFSEEQMERPLFFVVSEGHFYFLAPKLYEEQLSKYNFEINSYADGEDPYSKMDLKKNSVIAIDDQLWSVFTINLIRKFNPSNLIPASSLLKELRMRKDDDEINIMKEGLKIAENSFLQFLNDIKEGSSECKLADRLEEIFKENGTDGVSFKTILTSGPNTSMPHLRCTERKVRRGDVIIVDFGIKYRGYCTDTTRVVSLGNPSDEVKKIHEIVLNAQEAAERAKNGMKGKEIDSIARGIIAKAGYSQFFIHRTGHGIGIDVHEDPYISQDSEQVIEKNMTFTIEPGIYLPGKFGIRIEDMVVMGEKEAMPLNKLGKEIYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q7</accession>
    <name>F4B9Q7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1089</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775889.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q7"/>
    <dbReference type="STRING" id="933801.Ahos_1089"/>
    <dbReference type="GeneID" id="10600563"/>
    <dbReference type="KEGG" id="aho:Ahos_1089"/>
    <dbReference type="eggNOG" id="arCOG00321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="OrthoDB" id="98233at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053642">
        <property type="entry name" value="Zinc_metalloprotease_TldD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040952">
        <property type="entry name" value="Arch_mtprotase_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="100"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="214"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="445" mass="49543" checksum="5C3A56CDCF8F9CB0" modified="2011-06-28" version="1">MIEVLKKAENLGAIFADVRHYRYDELSLVVTENRKYVTTNGIDEGYSLRVLYNGNWGYSNSTKLGDEEVRNAINSAYGDEKVNITFLPSKKDKVVIKQEKPLSKSLEEIMSDLDKIKGELLKDERVKSVNVRYFHEGVKKDYASTEGREIYQEYVLSGISISAVAREGDIVASSFYSAYTYKGYPLEVFDINQMIETLRKRLNNQLKGKTPKAGEFDVVLGPDVVGVFSHEALGHLAEADLAVNGILGQLRGKKIAPDYVSVHDSPKMDYNMAIGLTPYDDEGVEGREVKIVEKGIVSEFLVDRYYSTFLGERPTGNARAEDFRSPILIRMRNTFMLPGDRKVEELISEIKEGYYIVSPLGGQTSPDGTFQFGIQEGYYIHNGEIGEPIRNMGISGYTIETLGRIKGVSKEFDVWPGFCGKGGQSVPVGTGGPYISVKVKVGGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R2</accession>
    <name>F4B9R2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775894.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R2"/>
    <dbReference type="GeneID" id="10600568"/>
    <dbReference type="KEGG" id="aho:Ahos_1094"/>
    <dbReference type="eggNOG" id="arCOG07286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444694_0_0_2"/>
    <dbReference type="OrthoDB" id="39701at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="187" mass="21595" checksum="5BEFCDBEA8C2E53E" modified="2011-06-28" version="1">MNRLDKNLLIYLPTSVIPIAGSTIYSIISINKIRDFVEALPYIFLGLPASLLTSAILTFEYFKQFIYVVLLIVVLAPFISSFAISRTYENVEVIPNEYYIEITLKVPISYSQFSDPNALFEKIFNKALKKMKNPYYYYKLRSLGNCSNLKVEVHEDRIVLRKRCGDTIVEINLSNKDEAYMKVSISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S7</accession>
    <name>F4B9S7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Argininosuccinate synthase</fullName>
            <ecNumber evidence="3 9">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argG</name>
        <name evidence="12" type="ordered locus">Ahos_1109</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3 9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775909.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S7"/>
    <dbReference type="STRING" id="933801.Ahos_1109"/>
    <dbReference type="GeneID" id="10600583"/>
    <dbReference type="KEGG" id="aho:Ahos_1109"/>
    <dbReference type="eggNOG" id="arCOG00112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_2"/>
    <dbReference type="OrthoDB" id="5877at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="162"/>
        </location>
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        <location>
            <begin position="170"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="6"/>
            <end position="14"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
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            <position position="114"/>
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        </ligand>
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        <ligand>
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        </ligand>
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        <ligand>
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        </ligand>
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        <ligand>
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        </ligand>
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        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
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        <location>
            <position position="171"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
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        <location>
            <position position="180"/>
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        <ligand>
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        </ligand>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="392" mass="44581" checksum="0E9527804D04D8FA" modified="2011-06-28" version="1">MKIVLAYSGGLDTTVSIRWLKEKYQADVITVSVDVGQKEDFKKLEERAYIAGSSKHYTIDAKEEFVEKFVKYDIIMNGLYEDVYPLSTALARPLIAEKVVEIAKKEGTEYVAHGSTSKGNDQVRFDLAIKAHMPDAKIITPARSWDMTRETEIKYAKERGIPIKEESSKYSIDENLWGRSIEGDIIEDPSKEVPEDAFELTRKTSTKNKEKIIITFEKGVPVALNGEKMPLLNLIDNLNKIAGSLGFGRVDHLENRVVGFKSREVYEAPAALCLIYAHKDLEKTVYTPLELRFKKSIDSQWSDLVYQGLWYEPLRETLQKVGVDMNNWVTGEVEVEIGENGLRILGRNSPYSPYSDKIASYNKGWYPTEEMAKGFIEIWGMHSLLARKVRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V3</accession>
    <name>F4B9V3_ACIHW</name>
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        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ordered locus">Ahos_2298</name>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95169.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MTH1187/YkoF-like"/>
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        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="101" mass="11568" checksum="67A8BD65E0A4E1B6" modified="2011-06-28" version="1">MNSMKYLVDISVEPIGTDSTSISRYVKLVYEVLKAKGIKFYPAPSMTTLEIDDITQLGYIIKDIDDTLAKEGVKRIVTIMKIDDRRDKENSIEHKLEVIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W3</accession>
    <name>F4B9W3_ACIHW</name>
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        <submittedName>
            <fullName evidence="6">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2308</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777094.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W3"/>
    <dbReference type="STRING" id="933801.Ahos_2308"/>
    <dbReference type="GeneID" id="10601816"/>
    <dbReference type="KEGG" id="aho:Ahos_2308"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_1_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000001">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="15973" checksum="DE3271AEB2764EED" modified="2011-06-28" version="1">MSSDYDPELEELLKKKIKQIMNTSKGMQENQKTQEELVAHLDSNTFDNFIKSHKVAVIDFWAEWCAPCIMLAPIIEELAKDYPQVGFGKVNSDENQDIAARYGVMSLPTVIFFKNGEPVDEVIGAVPREEIEIRIKELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y1</accession>
    <name>F4B9Y1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">Quinolinate synthase</fullName>
            <ecNumber evidence="2 9 10">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadA</name>
        <name evidence="11" type="ordered locus">Ahos_2326</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>iminosuccinate + dihydroxyacetone phosphate = quinolinate + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 9 10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777112.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y1"/>
    <dbReference type="STRING" id="933801.Ahos_2326"/>
    <dbReference type="GeneID" id="10601834"/>
    <dbReference type="KEGG" id="aho:Ahos_2326"/>
    <dbReference type="eggNOG" id="arCOG04459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_2"/>
    <dbReference type="OrthoDB" id="5931at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006878">
        <property type="entry name" value="PRK09375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573:SF0">
        <property type="entry name" value="QUINOLINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="QUINOLINATE SYNTHETASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="200"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE95197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="314" mass="34961" checksum="23068147D68F641D" modified="2011-06-28" version="1">MTDYAELIKRIKELKREKNAIILGHNYMDYAVQLVSDFTGDSYDLAVKAMKTNADIIVFAGVYFMAEQAAALNQDKLVLSPDPKAGCTLSDALDVETLKKFKEEYPNAPVVLYINTSIYAKALADYIVTSSTAVKIVKALDSDIVLFGPDANLANYVEKKTGKKIIKVPPNGRCLVHASYVKQFVEMARKKYPNAVLMAHPESPLEILEAADFVGSTNQMIKFAKESKYKEFIVATEIGMLNALKLQVPDKVFYPLISTESCACARCPYMAMITLDKIKNSLENEVTKVIVPKDVAEKAKEAFERTMKLLEKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z8</accession>
    <name>F4B9Z8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777129.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z8"/>
    <dbReference type="STRING" id="933801.Ahos_2343"/>
    <dbReference type="GeneID" id="10601851"/>
    <dbReference type="KEGG" id="aho:Ahos_2343"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565816_0_0_2"/>
    <dbReference type="OrthoDB" id="35765at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051011">
        <property type="entry name" value="Metal_resp_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43132:SF2">
        <property type="entry name" value="ARSENICAL RESISTANCE OPERON REPRESSOR ARSR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="10088" checksum="6A2584E2A34ADB8C" modified="2011-06-28" version="1">MLYKLDEVIENKGWDTRKKILELLQSKEMTAYEISKILELNYSTVKYHLELLERVGLVTVKKDKRNRYLYKANNNIKILNLIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA21</accession>
    <name>F4BA21_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA21"/>
    <dbReference type="STRING" id="933801.Ahos_1134"/>
    <dbReference type="KEGG" id="aho:Ahos_1134"/>
    <dbReference type="eggNOG" id="arCOG05995">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366034_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="10463" checksum="017BBDD33DA973E3" modified="2011-06-28" version="1">MKGRDLEGLAFLGKVESVNVEFCDDKKTLAKVVVKTTDGDEVESECIPVRAAGKISIVIKHYLRMGIGKYIITNEKTVGNVDTEGEDETEDRQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N1</accession>
    <name>F4B3N1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774837.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N1"/>
    <dbReference type="STRING" id="933801.Ahos_0025"/>
    <dbReference type="GeneID" id="10599465"/>
    <dbReference type="KEGG" id="aho:Ahos_0025"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_488874_0_0_2"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF2">
        <property type="entry name" value="HELICASE HERA CENTRAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="5">
        <location>
            <begin position="132"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE92920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="539" mass="60239" checksum="890ABE100EA7A0B7" modified="2011-06-28" version="1">MLAEGKTIGIVLQKSEANSIQGLITPEYEVSPGQLFLIKDNNKLSLARLENYEYINEFYDEKIPIVKNILSENTSFDLLNMNTVIKAEMSIIKRYGHNSSPMPGSLIKALPEEKDENFLSEFYGVKNVSDYVKYGRLAGSDIPLLLDLNSVTMHVGIFGETGSGKSYDMRYLIYLLSNLKIMGKNTALPMIIIDANGDYADFATINMDLVSGGRKWIKKYVIREPKSDTETRLTIDLSLFTPKDLADFIISLKYGGNEINSLQSNLLDYVISQHDQKEYNNLLGKEEGISLIQQEISSNQNIKELGFSSSTARAVISALEIFKDRIIKRYKLVSNSSSINEQILDVIWKEKGLAIIDFSADGSPGVDIPTKQLIVSYISRLLLNYLTKAKYSGSQRLIAFVIEEAQNYIPSNDYPISANITKESLVTLATQGRKFGVSLFLVSQRPAFVDKYVLSMLNTFFFHRIYHEDLKYVMSASGGLPESLAKGLTSLDTGYVIVSGLMSALKVPALVRIPWDPRLGSYAGSVERIDRVLIEDSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R8</accession>
    <name>F4B3R8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Single-stranded DNA exonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776021.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R8"/>
    <dbReference type="STRING" id="933801.Ahos_1222"/>
    <dbReference type="GeneID" id="10600698"/>
    <dbReference type="KEGG" id="aho:Ahos_1222"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_770787_0_0_2"/>
    <dbReference type="OrthoDB" id="36101at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="38459" checksum="0BB805CFF3509C65" modified="2011-06-28" version="1">MENFIKEPTPEEAKKLIESINKEESICIKIPYNVDAILAASLIIKYMENPSALSFSSSNCQLEFSEKEEGKFVRFKNTEVFIGNSSFSSLVPITTEDILPILTGISSSVVLERRNFSDWELSIMKNSENLGITIEKNLKIPSYHDAPIFLSLAESFDPYIPTVTGSRENAIALINEIGIDELSKLTELNEGALNTLIFKIVSLIMKINPKVTRDDIITDRVFYLNYDSLELAFSFIYFLDTIGSKILVDFALNQSIAGILINKFREIIGKGFSIGQVTEDKKFFIVDSNLKSPTLLQLILLQTQKVRKDKPIVIKDGNKLLTSRYFINTEEEGLIEVEGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S9</accession>
    <name>F4B3S9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Death-on-curing family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S9"/>
    <dbReference type="KEGG" id="aho:Ahos_1233"/>
    <dbReference type="eggNOG" id="arCOG06831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143272_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1870">
        <property type="entry name" value="Fic/DOC protein, Fido domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006440">
        <property type="entry name" value="Doc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053737">
        <property type="entry name" value="Type_II_TA_Toxin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01550">
        <property type="entry name" value="DOC_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="Fic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="1">
        <location>
            <begin position="20"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="143" mass="16483" checksum="618A440F48F99815" modified="2011-06-28" version="1">MQILAGLLNEFEEWRRGESDIEPTIIKIHYSIIRSDPNTEQGIINLDVIGIAIYSAIEDLNNYNDISRSLAVLTYHLITSHPFVDGNKRTAFVLLLNILYELFNKEIPQDLEEELIKTLVEVADNPPEEDEYAINKIRKIIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T4</accession>
    <name>F4B3T4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ser/Thr kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776037.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T4"/>
    <dbReference type="STRING" id="933801.Ahos_1238"/>
    <dbReference type="GeneID" id="10600717"/>
    <dbReference type="KEGG" id="aho:Ahos_1238"/>
    <dbReference type="eggNOG" id="arCOG01182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095575_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="212" mass="24456" checksum="B8E38FEAB420DA91" modified="2011-06-28" version="1">MLKGIPEENSNLVEISKFIYPKYSKDVEEELRSAGIYYAYSFGNVSLGRVNVIGKGKTGIVVYIGEGKVVKIRRTDSPKNSLELEAKIQEISYPSAPKVFDYGVNYIIMEYVNGSPLTRYDLRYLGDLLIRAKYLEDVHVQHEEISRPWKNVLVTQARTYIIDYDSASIKERPLNVTKILSAFGFYQLGEKYKRNEIEFEEIINFIKELRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W0</accession>
    <name>F4B3W0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyrosine recombinase XerA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xerA</name>
        <name evidence="9" type="ordered locus">Ahos_1264</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 'phage' integrase family. XerA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776063.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W0"/>
    <dbReference type="STRING" id="933801.Ahos_1264"/>
    <dbReference type="GeneID" id="10600745"/>
    <dbReference type="KEGG" id="aho:Ahos_1264"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_2_2"/>
    <dbReference type="OrthoDB" id="142231at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00796">
        <property type="entry name" value="INT_Rci_Hp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02055">
        <property type="entry name" value="Recomb_XerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050090">
        <property type="entry name" value="Tyrosine_recombinase_XerCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033686">
        <property type="entry name" value="XerA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040815">
        <property type="entry name" value="recomb_XerA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349:SF41">
        <property type="entry name" value="INTEGRASE_RECOMBINASE PROTEIN MJ0367-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30349">
        <property type="entry name" value="PHAGE INTEGRASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13495">
        <property type="entry name" value="Phage_int_SAM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4 5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="8">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="7">
        <location>
            <begin position="113"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="5">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="286" mass="33246" checksum="FD00C5ADB9B72D1F" modified="2011-06-28" version="1">MKLQLGSPPSDGNYLDAFIIALKAAEAGEGTVKLYSTAVKDFLEFIKKDPKSVTSEDVNRWIIHLMSKEGKIKGTEMKRARSVTLRNYVIAVRRFLKWIGVNVNPVIPRARRKEIRALNEDEINRLFEATRRLRDKLILRLFLDTGLRSKELLSLRVEDIDLKNRMIRVKETKNGEERIVFFTEETEKLLRKYLTKQGSSERLFPMTYQALYKLIKRLGERAGIKGLRPHILRHTFATIAIRRGLPLPAVQRLLGHKDIKTTQIYTHLVLEDLMQAYKKVFEENTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W6</accession>
    <name>F4B3W6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1270</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776069.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W6"/>
    <dbReference type="STRING" id="933801.Ahos_1270"/>
    <dbReference type="GeneID" id="10600751"/>
    <dbReference type="KEGG" id="aho:Ahos_1270"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_1_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF11">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="13"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="132" mass="15162" checksum="E26CD90048434294" modified="2011-06-28" version="1">MSEIESLIKEIAEKLQKKAESIKSPKYEPESVSEDKFDEILSKNKVVVIDCWADWCAPCHLYEPIFKKIAEKYKDKAFFGRLNVDENQKLADKYNVLNIPTTLIFVDGQLKDSLVGAVDENTLEDTIKKYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y2</accession>
    <name>F4B3Y2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetoin utilization protein AcuC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Ketone degradation; acetoin degradation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y2"/>
    <dbReference type="STRING" id="933801.Ahos_0051"/>
    <dbReference type="KEGG" id="aho:Ahos_0051"/>
    <dbReference type="eggNOG" id="arCOG00324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_0_2"/>
    <dbReference type="UniPathway" id="UPA00040"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045150">
        <property type="term" value="P:acetoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09994">
        <property type="entry name" value="HDAC_AcuC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003085">
        <property type="entry name" value="AcuC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0006">Acetoin catabolism</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE92946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="352" mass="39352" checksum="8E1A7B5A00E191DB" modified="2011-06-28" version="1">MENLRKTVFIWNENYLEYSFPGDHPFKSLRESMAKKLMEERGFFHEIDLENAEPVTESLLEKIHDKEYIEFVKKKSLEGKGYLDDGDTPAFKGIYEAAILRVGGSVKALEMIEKGYDVAINLGGGFHHAKKSSASGFCVFNDVALIAKLAEKKYKIAIVDIDGHHGDGTQQLLYDDNRVLKASLHMFHRNFFPGTGNYDEIGEGKGKGFTINVPLPPGTADDAYISAFKEVVVKKLEKFKPELIIIVEGGDSHFDDPLVELKLSTKGYSEVINEILSIARRFSSKIILLGGGGYNYEATARIWTITTAQLAGLDMSEVDLLHDCCFTSSTNFVKEKVNEVIKKLKEIHGLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B408</accession>
    <name>F4B408_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774889.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B408"/>
    <dbReference type="STRING" id="933801.Ahos_0079"/>
    <dbReference type="GeneID" id="10599519"/>
    <dbReference type="KEGG" id="aho:Ahos_0079"/>
    <dbReference type="eggNOG" id="arCOG03851">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165897_0_0_2"/>
    <dbReference type="OrthoDB" id="57238at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="12187" checksum="93246115C2DC0805" modified="2011-06-28" version="1">MEPFYFKSYDRVIGIAHDVKELENEVERLSKEDPAALEYHLKEGHIVSWLNYIGEKGLAEMLKGVVDPKEALARIKEYELLKSSTQMLPKSSKKEKKKKAFYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B487</accession>
    <name>F4B487_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776111.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B487"/>
    <dbReference type="GeneID" id="41368737"/>
    <dbReference type="KEGG" id="aho:Ahos_1312"/>
    <dbReference type="HOGENOM" id="CLU_1485825_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="181" mass="21298" checksum="900A786D56C7F645" modified="2011-06-28" version="1">MPSYEAISRIYKHYSFSLIIFSIISIFMTIFRQLIPNYIMYLVYSILLLYFIMFSSYLIISRYKIYTLFMIVNSIIALLLISLLFMYSTAYLVSPYVAGFILAVIAYITISLYMIKKYSAELTVNKYEILQSVFLGGYIIVNLITFTLIQYEKYGLLLNILSYALLFGFFLAIYYENKQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B5</accession>
    <name>F4B4B5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">8-oxoguanine DNA glycosylase/AP lyase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">8-oxoguanine DNA glycosylase</fullName>
                <shortName evidence="7">8-oxoG DNA glycosylase</shortName>
                <ecNumber evidence="7">3.2.2.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">DNA-(apurinic or apyrimidinic site) lyase</fullName>
                <shortName evidence="7">AP lyase</shortName>
                <ecNumber evidence="7">4.2.99.18</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="7" type="primary">ogg</name>
        <name evidence="9" type="ordered locus">Ahos_1340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the excision of an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine = 8-oxoG) from DNA. Also cleaves the DNA backbone at apurinic/apyrimidinic sites (AP sites).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type-2 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="7"/>
    <dbReference type="EC" id="4.2.99.18" evidence="7"/>
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        <property type="entry name" value="DNA_glyclase/AP_lyase_Ogg"/>
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    </dbReference>
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        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
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        <property type="entry name" value="PRK01229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22175">
        <property type="entry name" value="Ogg-HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thrmst_ogg"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="8">
        <location>
            <begin position="42"/>
            <end position="199"/>
        </location>
    </feature>
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        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="site" description="Important for guanine/8-oxoguanine distinction" evidence="7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="204" mass="23934" checksum="B869388F0FC0C2D5" modified="2011-06-28" version="1">MLRNLVKNVKLRAKVLERAEEFKLNNKAGEDVWFRELVLCILTSNSSFISAYTALNNLYDKLFSLNEDGISKVLKNSGYRFYNLKAKYLFNVRKYYGKLKAWIYPIANKDEYEAREELLQIEGIGMKEASHFLRNVGYFDLAILDRHILKFLSSYYFSYSKKSLPKKDYIYIESVLKSISISLNLPVGLLDLFIWYKATNKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C8</accession>
    <name>F4B4C8_ACIHW</name>
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_0124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C8"/>
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    <dbReference type="GeneID" id="10599564"/>
    <dbReference type="KEGG" id="aho:Ahos_0124"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of cell division"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
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    </dbReference>
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        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="459" mass="51830" checksum="E79EF1A4F33AE90A" modified="2011-06-28" version="1">MGKNVYLYKINVLGVKGGVGKSTISLNLGRFLAKNSKKVLLVDKDVLGFASYLSGIRGKGLLAKVVDGEEDYWNCSATLNFGEGKLTILKLFGDGDRLNEDLEKIHSNDSLREKFTKLYKSFLITDEYDYFIVDNIQGISAKSPLVIHELEVFYSTIPNVKNLRIYVSNYSINSIASTIKYAKSIEKEIKYEGFPLAFIVNLVPDNLDDLENAKNYAEKGRQEIGCKFSVVIPIYSELLGFSRSVSEMPEIKEINELGNKLLLEDFNTSPIINSATYDLSQILVDENSILIMGPVGSGKLELILKLINLAKNDRKIVIVSTNYRLESFLKDNKFVDFLPIHISQKYVEDRFNVRNIGEIIKISKELSTEIIKEIKNIDKVVIIIYRANDLSPASNCCDITAAKQEFWSSLINTIKFKKNTNLILIYDKIGQEYDVLSSVVDVVVETDINGKYKIERNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D5</accession>
    <name>F4B4D5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sulfur oxygenase/reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D5"/>
    <dbReference type="STRING" id="933801.Ahos_0131"/>
    <dbReference type="KEGG" id="aho:Ahos_0131"/>
    <dbReference type="eggNOG" id="arCOG06967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_904908_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011661">
        <property type="entry name" value="S_Oase_red"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047630">
        <property type="entry name" value="SulOxRed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07682">
        <property type="entry name" value="SOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="300" mass="34290" checksum="96D6410C6B7C907F" modified="2011-06-28" version="1">MAELKNEPKTFEMFASVGPKVCMVTARHPGFVGFQNHIQIGILPFGNRYGGAKMDMTKESSSVRVLQYTFWKDWKDHEEMHRQNWSYLFRLCYSCASQMIWGPWEPIYEIIYANMPINTEMTDFTAVVGKKFAEGKPLDIPVISQPYGKRVVAFAEHSVIPGKEKQFEDAIVRTLEMLKKAPGFLGAMVLKEIGVSGIGSMQFGAKGFHQVLENPGSLEPDPNNVMYSVPEAKNTPQQYIVHVEWANTDALMFGMGRVLLYPELRQVHDEVLDTLVYGPYIRILNPMMEGTFWREYLNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G1</accession>
    <name>F4B4G1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G1"/>
    <dbReference type="KEGG" id="aho:Ahos_0157"/>
    <dbReference type="eggNOG" id="arCOG07186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_945338_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="300" mass="35779" checksum="F6DBADF945D2D7A2" modified="2011-06-28" version="1">MALDLQLGLWVIICFQSFMVVDSSSKEYSYFFDTNILIIWLYEKNSDSAKRLNEILDNSTRINRNVLTYNLEELYEIIGDSYIIFYYILINKILPRWSINSVYERNRILSEIDNNFDKIYEEVVNKYNINPSDIRKFISRLFFNLNKQRIVLMNQSELKDRFTNNFASDLIDYISGITRLLTNTFNVVSFPKVLMTEDLNMEFQLIEEGISSYIKVAGKEPSRYDQLIYKTLYLLLKLNEYDQIVFITNDTDFDRMHRRVIAHLQRISNSNSKARDHALRVLNIFNRLKISNLFYLTRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H5</accession>
    <name>F4B4H5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Binding-protein-dependent transport, inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0172</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774981.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H5"/>
    <dbReference type="STRING" id="933801.Ahos_0172"/>
    <dbReference type="GeneID" id="10599612"/>
    <dbReference type="KEGG" id="aho:Ahos_0172"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_1_2"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="116"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="338" mass="38639" checksum="4ED927590B05FAC8" modified="2011-06-28" version="1">MASSNTVQTSRPFIRIVIRRLIARLVLLFGVINFNFFIFQVIPQLVGLNPLEFYVPPSFKTSLNRNAIIEGLEKSYGLNQPICIRYIDYIKDLFTFNFGESLYYQEPILKLIEQNFPVTAEIVIPSLIITTIMAIFFGIYSATKEGKVSDHVISNAAIITYFIPAFWLGFIIWYYLTIQYNLFPSSYTFAIIESRKDPLAIYTVLIPPILTLSFTSFGVRTILMRNNSIDVFNQDFVTILKAKGVPRSRILFRHVFKNSFLPVFTRVGIDFAFLLSGVVFVCDVFNISGLGTLLVTAAENFDIFLLEGDFYIISLFAIVVLTLMDFIYILIDPRVKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I4</accession>
    <name>F4B4I4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I4"/>
    <dbReference type="KEGG" id="aho:Ahos_0181"/>
    <dbReference type="eggNOG" id="arCOG07265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136101_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="252" mass="29435" checksum="3FBEB5DB8BAA4D4F" modified="2011-06-28" version="1">MLFRRQYKTPTIMLSKIVKLPITDNEFIKSWFSDLNNIVNCIPSALKDGNFILIEIPSIIINKKIKMKYYIRIDKNFCQLTLEGKEDKMKISVILDDEPFAQIYYEGNNEGSFYKIFDEIDRKLKEKIHVDYVNYKENFMNSNLSEHLKRISFVTKLVAKSKLILEKEINTNDLISSLEELIASCNNYPVLYISGYGDGVFRLIFINGEFRGIYINYEGKESYTEEELKNLKGKFKISVYSAKLGDILSNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I6</accession>
    <name>F4B4I6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774992.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I6"/>
    <dbReference type="GeneID" id="10599623"/>
    <dbReference type="KEGG" id="aho:Ahos_0183"/>
    <dbReference type="eggNOG" id="arCOG02221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149092_0_0_2"/>
    <dbReference type="OrthoDB" id="30561at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39677:SF4">
        <property type="entry name" value="RIBONUCLEASE VAPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="113" mass="13083" checksum="C1FE2A848BB72467" modified="2011-06-28" version="1">MTVVDSGVLVEYINESGEYHDKVKKLLEENNTLYITPITLSEVMYVSYRIYKASGLNDANKYARDFVEWLSYKLKVTEINKDITFDAGEIKKKYDIAFPDCYVIATANYKERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I9</accession>
    <name>F4B4I9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="8">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">leuS</name>
        <name evidence="11" type="ordered locus">Ahos_0186</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774995.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I9"/>
    <dbReference type="STRING" id="933801.Ahos_0186"/>
    <dbReference type="GeneID" id="10599626"/>
    <dbReference type="KEGG" id="aho:Ahos_0186"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.20">
        <property type="entry name" value="Class I aminoacyl-tRNA synthetases (RS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.720">
        <property type="entry name" value="leucyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008957">
        <property type="entry name" value="PRK12300.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="9">
        <location>
            <begin position="9"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="9">
        <location>
            <begin position="554"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="10">
        <location>
            <begin position="680"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="607"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="610"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="920" mass="105528" checksum="C1A267D431F245FD" modified="2011-06-28" version="1">MDFNAIAEKWQKVWEEKGIFEAKVDERKKKYFITVPFPYTNSPMHIGHGRTYVTADIYARYMRMKGYNVLFPFAFQFTGTPILSVADAIKRGDQDIIDFFKNVYEIDEEKIKDLSDPYKLAEYFKQEMEKTAKAIGLSVDWRRSFTTVDDRFAAFIQWQFKKLKDKGKLIKETDAVGYCPRDQFPVGMHDTKGDVEPEIEKLDVIFFEGDYFYPVATSRPETVFAGVGVAISPLTTYVIAEYSGKRILLSKEALEKLSYQKELKKIGEVKPEELKGKEAVNPVTRKKVKVVLSKLVDPSIGTGLIMLTPAHDPIHKIIAEDNGIEDYYSVISSPGLPEIPTEEIDVRDMAELKDFADQIYRTEYYKGVMKEVSAYVPDYMKQYVKEMISGKPVKDARISTVELLNSIGRHDTIYEIMNGPIYCRCGAQIVVKVIKDQWFIAYDDPEWKMAVLNSLDNINFVPSEVRKDIEKAIFNLRRRAVGRSRGLGVKLPWDESEIIDSLSDSTIYTAFYTISHLLKKANDKLFDYVFLGLGNPEEISKELGISKEEVEELRREYNYWYPVDSRHSGRDLVQNHLPYYIYNHLEILGKLPRQIVINGFVRVGGKKMSKSFRNVYPLSKAIKEYGVDPVRIALATPKLSEDVEFNSSVVTSIADQLKRIYSLIVELLEAKGENNFGIAEKWLSSIMSEKIKTVDEKMRNLDFFEAYNIILYDIYNDFKDYLDFTNGINEDLIKKSISAWLRMIYPAAPHIAEELWSKAFPGLVAEQEYPKPEEFTIYPEAVVEKGYVDYVISEIKELERIVGEGEKAVIYVNNDLSLAKDTAKAIEEGQSLFEFARGNEKLEKVYSTIKAYDGIFRKSLLTLQEFDEIRTLANYANYIMSKTNLGQLSVYDSNDPNVPDFKGKKSQALPLSPAIVIFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J4</accession>
    <name>F4B4J4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0191</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775000.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J4"/>
    <dbReference type="STRING" id="933801.Ahos_0191"/>
    <dbReference type="GeneID" id="10599631"/>
    <dbReference type="KEGG" id="aho:Ahos_0191"/>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270888_0_0_2"/>
    <dbReference type="OrthoDB" id="38551at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="102" mass="11861" checksum="52EA106069DAD4E3" modified="2011-06-28" version="1">MQKTFIDKNINIQKLVQDLQNSLSIDGWEVTTNQYAQNDYLLRGQKKGHFHKVEEILIRVRGDPNNVLITIDEENIGTFGRAWINLKLLGEIERKVNDGFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L1</accession>
    <name>F4B4L1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoenolpyruvate synthase</fullName>
            <shortName evidence="12">PEP synthase</shortName>
            <ecNumber evidence="12">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_1358</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>pyruvate + ATP + H2O = phosphoenolpyruvate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776156.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L1"/>
    <dbReference type="STRING" id="933801.Ahos_1358"/>
    <dbReference type="GeneID" id="10600845"/>
    <dbReference type="KEGG" id="aho:Ahos_1358"/>
    <dbReference type="eggNOG" id="arCOG01111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_6_2_2"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01418">
        <property type="entry name" value="PEP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005057">
        <property type="entry name" value="PRK06464.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030:SF1">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000854">
        <property type="entry name" value="PEP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="14">
        <location>
            <begin position="3"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="13">
        <location>
            <begin position="354"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="15">
        <location>
            <begin position="451"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE94241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="763" mass="85167" checksum="B6944DFEDB42EF60" modified="2011-06-28" version="1">MIELAGGKGANLGELVSFDIRVPPGFVITSKAYSYFIAYNNLESKIKSILEEKDSATASEEIKKLIISSQVPPDLENAILSSYDELAKKVGKEILVAVRSSATAEDIENASFAGQQDTYLNVSRSELIQKVKEVWASLFNERAIEYRKTKGIDSTKVEMAVVVQKMVNSRSAGVMFTLHPATGDSRYIVIESSWGLGEAVVGGKVTPDEIVIEKSTLRIVEKRVSHKILKYVYNSQKNANEEVDLSNSPEADKISISDEEAIELAKLALKIEEHYKRPMDIEWAIDADLKFPDNVFIVQARPETFWSSKKATKEEKEVTSVENRKVLVKGLAASPGIASGIARVILDVKEAGNFKKGDILVTRMTDPDWVPIMKIASAIVTDEGGITSHAAIVSRELGIPAIVGTKEATKVIKDGQEITVDATRGIVYEGRIVEEVEEQKPQVAQGISGISRDTLMSLYPITATKIYMNLGQPDVIEKYVDLPFDGIGLMRIEFIVSEWIKYHPLYLIKIGKPEEFVNKLADGVARVATAIYPRPVVVRFSDFKTNEYRRLIGGEEFEPEERNPMLGWRGVSRYVSPQYEPAFRLEVRAIRKVREEMGLKNVWVMFPFVRTEWELKKALKIMEEEGLQRSKDFKVWIMAEVPSVIVLADVFAKIIDGFSIGSNDLAQLTLGVDRDSEILGRMGYYDERDPAVLRSMKKLIRIGHKYGATVSICGQAPSVYPEVAEFLVRAGIDSISVNPDAVISTRRNVASIEQKIMLEKLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L7</accession>
    <name>F4B4L7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aspartate-semialdehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1364</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776162.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L7"/>
    <dbReference type="STRING" id="933801.Ahos_1364"/>
    <dbReference type="GeneID" id="10600851"/>
    <dbReference type="KEGG" id="aho:Ahos_1364"/>
    <dbReference type="eggNOG" id="arCOG00494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="OrthoDB" id="38238at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="350" mass="38761" checksum="649C291389E6782B" modified="2011-06-28" version="1">MDKLKVSLLGSTGMVGQKMVKMLSNHPFIELTKVSASPAKIGKKYEEAVKWVEGEIPENVKDMRLVSTEPEDHKDVDVVLSALPNELAEDIELKLVRAGKIVVSNASPYRMDPEVPLINPEINWQHLELVKTQQSKRGWKGLLVKNPNCTAAIMSLPLKPLTELITQRKIIMTTLQAVSGAGYNGLSFMSIYNNIIPYIKGEEEKIPKEIGKMLGQVQDGQILNAKINARVTSIRVPIKVGHMGVINILLDESDKIDVEEIKRELANFKSLPQEKNLPTAPKKPIIVNEDESRPQPEIDLKIENGMAVSVGRISVENNVLRMVVLGDNLVRGAAGITILTVEVMKELGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N3</accession>
    <name>F4B4N3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1059 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776178.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N3"/>
    <dbReference type="STRING" id="933801.Ahos_1380"/>
    <dbReference type="GeneID" id="10600868"/>
    <dbReference type="KEGG" id="aho:Ahos_1380"/>
    <dbReference type="eggNOG" id="arCOG05278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067863_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13066" checksum="D0E17690EFA45BFC" modified="2011-06-28" version="1">MKYTFSCADIGMNCGFEIINAGSEEELLEMLKTHAKMDHGITSIPPELIDKIKKAIRKSGKYSFSCADIGMNCGFEIIGASSEDELLQQLSIHARMSHKMSNIPQDTINAIKQKIKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N6</accession>
    <name>F4B4N6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776181.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N6"/>
    <dbReference type="STRING" id="933801.Ahos_1383"/>
    <dbReference type="GeneID" id="10600871"/>
    <dbReference type="KEGG" id="aho:Ahos_1383"/>
    <dbReference type="eggNOG" id="arCOG05980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848917_0_0_2"/>
    <dbReference type="OrthoDB" id="36284at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="356" mass="41154" checksum="F86662126050C2CD" modified="2011-06-28" version="1">MDEELPPNPRDDEKAFVGPSMINFSIPFANIDTVKLSDEDLNIAQLPQLLKLSNAKKVLWKYKAKIIGVDGSEILAEGEDIIKGPFVVFTPLEINAIPWSFTKINEKSLINLVKDLIPCDEGEGYFNPSPWDRKALIDEKWYYFRPGEITEKLNIPTQGYELAGYKIESNFYNPKFYFLNPFYIEESRYPISASSFISLQSDTALSIISSDPFNIKFNLGKIEIESERQVYIIKSRIWKEIKPARISWDLKNNIIRLDCKPKYNVSIYKIEPSSVIPLYFDYKNGELLLILENFSDDDVISTLIFSGRIDSATADGEELVTEFDRVRIPIRKWGIKNVKIKIRRLIEPYLRRKIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S5</accession>
    <name>F4B4S5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif5a</name>
        <name evidence="13" type="ordered locus">Ahos_1422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776220.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4S5"/>
    <dbReference type="STRING" id="933801.Ahos_1422"/>
    <dbReference type="GeneID" id="10600914"/>
    <dbReference type="KEGG" id="aho:Ahos_1422"/>
    <dbReference type="eggNOG" id="arCOG04277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="OrthoDB" id="23689at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04467">
        <property type="entry name" value="S1_aIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000038">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000334">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003076">
        <property type="entry name" value="PRK03999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0385">Hypusine</keyword>
    <keyword evidence="6 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="12">
        <location>
            <begin position="69"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="11">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="14537" checksum="623EE0CECB2EF536" modified="2011-06-28" version="1">MGVNYAKVGDLKEGNYIVIDGEPCRVVEITKAKTGKHGAAKANVVAVSIFTGAKKTLMAPVDDQVEIPIIEKHVGQILADMGDKIQVMDMDTYETFEMEKPKEEDLASKIRPGAEVEYWTIMGRRKIVRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>F4B522</accession>
    <name>F4B522_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1441</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B522"/>
    <dbReference type="KEGG" id="aho:Ahos_1441"/>
    <dbReference type="HOGENOM" id="CLU_2678649_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="74" mass="8963" checksum="EEAF5C12EB4ADB29" modified="2011-06-28" version="1">MLVVKNVKKILHWPYPFLKLRVKVRNSWYRNFLKEFLFISARIHFDSVYPWRILTFNSATGTPLKGSYTFTHWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B528</accession>
    <name>F4B528_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B528"/>
    <dbReference type="KEGG" id="aho:Ahos_1447"/>
    <dbReference type="eggNOG" id="arCOG07849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850509_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="15873" checksum="38D4F0D4C733BECB" modified="2011-06-28" version="1">MLNDMKVVKYYVDVKVELKEGKNVDSEKAKLEGLLKRFSKKKKGEDKEEPKLTVTDHTIELSGIRFRERIGFRNFVDELAQKYEAKCTPLNEANGKITVQCESEKAKISFEANIVRFKRPKKEEGEKKEEAKTSGSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B534</accession>
    <name>F4B534_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YncE family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776251.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B534"/>
    <dbReference type="GeneID" id="10600949"/>
    <dbReference type="KEGG" id="aho:Ahos_1453"/>
    <dbReference type="eggNOG" id="arCOG02560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_854190_0_0_2"/>
    <dbReference type="OrthoDB" id="43870at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051200">
        <property type="entry name" value="Host-pathogen_enzymatic-act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011964">
        <property type="entry name" value="YVTN_b-propeller_repeat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02276">
        <property type="entry name" value="beta_rpt_yvtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197:SF3">
        <property type="entry name" value="DIHYDRO-HEME D1 DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47197">
        <property type="entry name" value="PROTEIN NIRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="35255" checksum="2F3D0DBD44939991" modified="2011-06-28" version="1">MVRELSFLVLFILISVSSSIVSIEVGNVPSFILCNSGYIYVTNYNSSTVSIIDPSNNAVISTITVGSQPISMIGAGNKIYVALAGCDKIVVLENNKIIRSINLTSSPYYMAYDPKNQELFVTEPEINSLAIIKNYSLIKTINLGYQPDAIAFDPKNCLLYIGGGYNGVVYVYDIEGKLNTTYYVGGQVVYVNYCDGNIFVTSWKNNELTIINSSGVFHFNAGLGPYDAIYDPSDGYIYVSDVATGSILVLSLSGSIIHNISVGGRPSILMYKNGNIYAVNSLSNDVIIIPQVPPPPPPYCLYYLIAGGIAVLVLIGYFVIKKEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B570</accession>
    <name>F4B570_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776287.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B570"/>
    <dbReference type="STRING" id="933801.Ahos_1489"/>
    <dbReference type="GeneID" id="10600989"/>
    <dbReference type="KEGG" id="aho:Ahos_1489"/>
    <dbReference type="eggNOG" id="arCOG03832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_0_2"/>
    <dbReference type="OrthoDB" id="8895at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="1">
        <location>
            <begin position="46"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="410" mass="47869" checksum="73558FBB28FA6818" modified="2011-06-28" version="1">MKLIRDPIHGYIEISDKMNKIISDPYFQRLRYIKQTAMAYMVYPSMLHSRFEHSLGVMHLAREFARYVISNSGLKDEEGLIQMIALTGLLHDIGHVAFSHTFENFLLLANQVYGLKVKEEGKKTHVNYGTYLIENVLGSIIDKEFTEFYQDPVKFIIDVLKESPRNYEEKLALSLISNYVDADRSDYLLRDSYYAGVSYGRFDIERLKRFLVFIDGKLAIYSKATPIVEQFLLSRMYMFENVYFHSVTGVYNAILSHAMVEMLEKNIISLPTEPKDFLQLLDIEIYSNLDKVKEEFKNAIMFRQGYKRIKYDITGKCLEKFNEIKDEIYEDMKESQGLFLYHEFNDVPYEEKEEAVYIFDGERVEKLTSVSPIIRSLSEIKKAVIAYSSKAEKVAEKYENVIQECKTLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C3</accession>
    <name>F4B5C3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SMP-30/Gluconolaconase/LRE domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775133.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C3"/>
    <dbReference type="STRING" id="933801.Ahos_0326"/>
    <dbReference type="GeneID" id="10599767"/>
    <dbReference type="KEGG" id="aho:Ahos_0326"/>
    <dbReference type="eggNOG" id="arCOG05370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036110_3_1_2"/>
    <dbReference type="OrthoDB" id="341532at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="4">
        <location>
            <begin position="15"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="102"/>
        </location>
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        <location>
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        </ligand>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="287" mass="32267" checksum="4C4E7A5D62040270" modified="2011-06-28" version="1">MLPIVEVFSKYKAKLGEGPIYDRDNDRLFWVDIEGKKIIIEEGITKNSVEKIVNLPDLVSSLCVINETEVIATIRHGFYILNINTGGLTKLLEIETDLKDNRFNDGKCDPLGRYWAGTMNMKKSGPTGNLYKLESNKISKILGGLTVSNGLGWSPDNDKMYLIDSPVRKVYVFDFNLNRGEIYNKQVLIDFGSEPGNPDGMTVDEEGYLWIAHWGGGKVSRWSPNGKKVFEIKMPVTYVSSVTFGGEDLSTLFITSASKDDEVQSGKLFMVDVKIRGLPSYKFFYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="InterPro" id="IPR039709">
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    <dbReference type="PANTHER" id="PTHR42244:SF2">
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    </dbReference>
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        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="86" mass="10409" checksum="7DEB97446F119314" modified="2011-06-28" version="1">MFTKKENNMTTTVISIRVDEKLKKDLEELGIDYAELVRKYLEEVVRKEKKRRELEEADKIREELLKTHGYYSSSAELIREDRDDYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <subcellularLocation>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
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    </feature>
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        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE94384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="33677" checksum="58890AE9B134F53F" modified="2011-06-28" version="1">MAELIKFIGVLTTLIIAAELLARGTENMERRFGQGIAGGVILGFLTALPETVITVYALLNREYEVAIGSAIGGNVILFTLGIGLVGLIYSFSWNRSLTMNYDYNVENNFLILNTIILAIIVFYGRFNILTAIILSSIYIFYIFYRIKKGKDEKENDKITNLSKSVIELVIGAILILAFSDLFVKCIVAISKEFNLPVAWTSLLITPIAAELEEKISGIRLAMRNLNGGSLAIVSFVGSKIENSTLLLGLIGFFTSYSFYNSLPELISAVIANVIGIGILLDGKVIKYESVILIALYFIIIYLDLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G5</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776304.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G5"/>
    <dbReference type="GeneID" id="10601006"/>
    <dbReference type="KEGG" id="aho:Ahos_1506"/>
    <dbReference type="eggNOG" id="arCOG06010">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110562_0_0_2"/>
    <dbReference type="OrthoDB" id="27450at2157"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR031594">
        <property type="entry name" value="OFeT_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16955">
        <property type="entry name" value="OFeT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="208" mass="22947" checksum="64FFBE9F497F2E91" modified="2011-06-28" version="1">MDIAVLLASLGISILELSEAGAVAVIFNGIYKNGKPYIYGLAGVLTVLIPVFTLGRYIVLLPLEYVLIVAGVILFYFGYKLIRSARRYFKGIKIVHKEEGEETLTTVYVVGFTEALEAGLVILALIPQSYISALTGTLLASILVIALLFVLKAQIMKIRVPQLKYVLSALLFSLGTLFFGEAFFDIDELYLGLFFVIYLGVNYVIIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H8</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1453 domain-containing protein</fullName>
        </recommendedName>
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Liu S.J."/>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="9016" checksum="9778E0AE19812E9A" modified="2011-06-28" version="1">MLIKVDKKGRVYLPKEVREKFKSEEFFLVTLPSGILLVPRVDDPLKALEEEGKKLPNVDIKVLRKVIQEEAEKEVGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B619</accession>
    <name>F4B619_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1639</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B619"/>
    <dbReference type="KEGG" id="aho:Ahos_1639"/>
    <dbReference type="eggNOG" id="arCOG03708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="172" mass="20017" checksum="ABDDE17B2795CF91" modified="2011-06-28" version="1">MLNAVKYSLYVLLYMQELPKPWFNLEAYKKIRLEEAKFEVEIAEKFLQEGLIRNAAGKVFQAWKSLLAALLTDKRDVLVKKYPKKKSLGRRKIEFADWIIAIVPTSYLEELSLILGKEINLLTEKALFIHEYQYNGPDKEAILSPFRSDEIAAENIKILIDEIKSILQKVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B621</accession>
    <name>F4B621_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776434.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B621"/>
    <dbReference type="STRING" id="933801.Ahos_1641"/>
    <dbReference type="GeneID" id="10601141"/>
    <dbReference type="KEGG" id="aho:Ahos_1641"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="299"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="383" mass="45069" checksum="72BAA8B2C5C9D70B" modified="2011-06-28" version="1">MARRGNKAIRATVSMKIALSDSLLVLVNNYVKALRFTIFWLKENVPNPNEKGVLGKIHEELYTRLREEYNLPSKVAEDCYRDALSIYKAWYNNPRRGRFPRVYKPTVWLTPRASYNVSFEAMIVRIASVGDLQILGYPRNLREYMSWKMKEARLVIRNDKAFLKVVFEKPGEESVEPKESIAVDINMSEIVVGKDDEHYVRIPTRLHEVHHWKSLAENLQKKYPRRWRENKRILYRVRSFHLKAKRIMEDFARKVGKWVVEIAGDFGANAIKLESLKNLVKNVDKLPKEFRDKLYLMQYRRIQYWIEWQAKKHGILVQYVNPSYSSVSCPKCGRRMEEKGYRLFKCSCGYENDRDVIAIVNLNGRGSLTLSTAPQMRDARANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B631</accession>
    <name>F4B631_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1651</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776444.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B631"/>
    <dbReference type="STRING" id="933801.Ahos_1651"/>
    <dbReference type="GeneID" id="10601151"/>
    <dbReference type="KEGG" id="aho:Ahos_1651"/>
    <dbReference type="eggNOG" id="arCOG00945">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_3_1_2"/>
    <dbReference type="OrthoDB" id="5620at2157"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="ARYLSULFATASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="63"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="396" mass="46161" checksum="64F4C15620A93399" modified="2011-06-28" version="1">MLSKYNIFLDDYKIMFNTLTGYAIRLTDEEIEKLKKGEVPEELKDIIEEGFSATFDEFLQKFKKEVLEPTLVLTYRCNFDCVYCFQKAFRNNSSVSDKVVRGFIKYVRTHANGRKVRVTYFGGEPLLELRRIKEISTQLSDLKYSFSIVTNGSLLTRHVFDELKSLGLTHVQITLDGPREVHDKRRYFVGGKGSYDIILKNLKEIQDEVNVVLRVNIDVNNLDSFRDLLRDLKQNGITKVRLDPHLVHDNVFRNEYWDYTFPKDEEGEILVKLWETAKDEGFEIPQDVFRLGLCVAHFDEDIVVDPFGNIYPCWAFTGNPLYVKGYLTEDGDVVIYEKLSGERALNLWKKCKDCPYMPLCLGGCRFFSVLNRKGYDGIDCRKKSYEAIMKLLKYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B682</accession>
    <name>F4B682_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0482</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775286.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B682"/>
    <dbReference type="STRING" id="933801.Ahos_0482"/>
    <dbReference type="GeneID" id="10599922"/>
    <dbReference type="KEGG" id="aho:Ahos_0482"/>
    <dbReference type="eggNOG" id="arCOG01707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="119"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="229"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="378" mass="40723" checksum="7876D46A6F19984C" modified="2011-06-28" version="1">MFELSKELEEYRNKVRDYTQKVVKDYAKTMDETNDGGDKIVKDFGEMDLLGMKIPTKYGGLGLGEIAFAIATEELGSESGGASHSLHTQLNALQLLVSVGGDSAKDWIERGVKAKEIYAVALTEPAAGSDLGALQTTAKPDGDELVLNGEKIFTSAASFSTKMVVLARTSGNPGDKNGISLLLIDSKLPGIEIHKLDLMGIRGAGVSYVKFNNVRIPKDSIIGKEGDAFRGAIKALMISRNGYAGIAVGIARGAVEEAINRAATRKQFGKALIEQEWIGFNLADAYIKVEAARLLTWRAASLLDKGVEALTEASMAKYFAAVTAAETTRTALHIFGGHGLNRGSKVERLYRDAKIMEIAEGTNEMQLLAVSRSFQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A3</accession>
    <name>F4B6A3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron dependent repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775307.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6A3"/>
    <dbReference type="STRING" id="933801.Ahos_0503"/>
    <dbReference type="GeneID" id="10599944"/>
    <dbReference type="KEGG" id="aho:Ahos_0503"/>
    <dbReference type="eggNOG" id="arCOG02100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_4_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron dependent repressor metal binding and dimerisation" evidence="1">
        <location>
            <begin position="63"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="130" mass="14966" checksum="BDB498D002FAA6AF" modified="2011-06-28" version="1">MRYSHRELQYLLVIKRYNDSGKPARLTAIAKDVKVAPASAFEELNHLAEKGLVTKDKNEIWITDEGKQYIMKAIRAHRVIESFLVKLGMSKDEACKYSKQFDLMVPEEIIEKLYIFLGKPSNCPHGEEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C5</accession>
    <name>F4B6C5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bacterio-opsin activator HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776460.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C5"/>
    <dbReference type="STRING" id="933801.Ahos_1667"/>
    <dbReference type="GeneID" id="10601167"/>
    <dbReference type="KEGG" id="aho:Ahos_1667"/>
    <dbReference type="eggNOG" id="arCOG02271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_1_0_2"/>
    <dbReference type="OrthoDB" id="202021at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF2">
        <property type="entry name" value="HTH BAT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="145"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="203" mass="23007" checksum="33E30495183666BE" modified="2011-06-28" version="1">MYRSPFEVTLLIEDHPCEVMKIISLAGLKASVENVKLGEQTTDHIVSFEKEIGKNEVLKLKSNSVKVLKLSETKVWVRTNGCAVCRILYTSDIVVEKIKVLKERTLLYTLLVLASSSFNEFLTKLSKEGVKVTVLNVSEISSAELTKRQMEILQLAYKLGYFNDERGITLSQLADKLGISAPTLEEILRRALRKVVKYYLDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G6</accession>
    <name>F4B6G6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thermopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776501.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6G6"/>
    <dbReference type="GeneID" id="10601209"/>
    <dbReference type="KEGG" id="aho:Ahos_1709"/>
    <dbReference type="eggNOG" id="arCOG03673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058361_0_0_2"/>
    <dbReference type="OrthoDB" id="43906at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="42858" checksum="CCF9EB80D2F6F5CB" modified="2011-06-28" version="1">MKVLFLLVILIIFIPTVYAGYDYGYEFGYVQTCGIQSIVSLYNISLEPGSPGISIQENVCLNINGSNVFVQNVIEPVMLTRYGYDVIWTTSVYYDGAYHMFCHSGIIGYTFNITTIWTNTSNALCIEFFISNTTLTLNKTCVIMGKFFGIIYSGYNAGTVIGGYGNSAVAELAKGFNVSIREYYRYNNNWYVPPVAYSGVWSTGESAINGYAYFSKGTVWITYGNAGIQELYNFSVVIVNNTVYTFPRGSLWIANGRFFVNFTPLENVTIRPFYYFNYSFIPKKEILISFDNYTEIDCVKSKSFYLPFPEVVYFYENGSCVGKFISTNITYIASMVQSSYTKSIAHTINVNKTDKIAIVIIGILFITLIFLLFRKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H5</accession>
    <name>F4B6H5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776510.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H5"/>
    <dbReference type="STRING" id="933801.Ahos_1718"/>
    <dbReference type="GeneID" id="10601218"/>
    <dbReference type="KEGG" id="aho:Ahos_1718"/>
    <dbReference type="eggNOG" id="arCOG07343">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346504_0_0_2"/>
    <dbReference type="OrthoDB" id="55583at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="190" mass="20991" checksum="04F5574A53EBA323" modified="2011-06-28" version="1">MNLPILPLAIGFFGLGTGYFVYGGVEVSGYPQSSESLNKSLGQWGIWMPGFMQFITAIILWAYIAMYDVTPPNALYMAAVAFTAYGVHWFALGLNRHYGGDARVDGYMAVAFLWISIIGALVFYEVKDNPVGILFTLLALVYISDIPASLLASRQWTKVKGYFHLITGVWLMYLTFAAVTSFALGWSLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I4</accession>
    <name>F4B6I4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR-associated protein Cas6 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776519.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6I4"/>
    <dbReference type="GeneID" id="10601227"/>
    <dbReference type="KEGG" id="aho:Ahos_1727"/>
    <dbReference type="eggNOG" id="arCOG01440">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929440_0_0_2"/>
    <dbReference type="OrthoDB" id="36576at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019267">
        <property type="entry name" value="CRISPR-assoc_Cas6_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10040">
        <property type="entry name" value="CRISPR_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR-associated protein Cas6 C-terminal" evidence="1">
        <location>
            <begin position="138"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="252" mass="28953" checksum="CD79AF05D445A203" modified="2011-06-28" version="1">MFSSYTLTVKMKYERGKYNNFTGKISKIVLLTEFPELEILFKPAKGFFKPIHVSPPISKEAVYPFYERGVLREVSLDGEYKIRIGVPDELSKGIEEKIKNDIGVRTRIKVEGAILEFTLENFEKNEFKIGDRVKLCFSPTLLSNPFIINRDYRKFLPSPSAVFWIPYALLKNKNSVTPQEVADIEGRITETWKSRIKTIWIPYDTRKEPVMIGKVEYKLLRNDDETKKLVETALTLGVGSSRASGFGHVELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J2</accession>
    <name>F4B6J2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775318.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J2"/>
    <dbReference type="STRING" id="933801.Ahos_0514"/>
    <dbReference type="GeneID" id="25395603"/>
    <dbReference type="KEGG" id="aho:Ahos_0514"/>
    <dbReference type="eggNOG" id="arCOG02115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253639_0_0_2"/>
    <dbReference type="OrthoDB" id="56850at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012440">
        <property type="entry name" value="DUF1641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38433:SF1">
        <property type="entry name" value="DUF1641 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07849">
        <property type="entry name" value="DUF1641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="27823" checksum="EA05E36BCE36E1A4" modified="2011-06-28" version="1">MGAIVNDKTLSLINNFDKLTGLLDFFMDAETMDNIKYALSLVGKLKSTGIIDPIIGILQDEEYMSKIMGAIVNDKVLELIGKWNNIVDLISDLTDDETLSAIKSVVGLLKDLNKTGILDPIKGILHDEEYMGKIMGALINDFTLNLISNWNQITSDLSKIDLTNFKYYIQLINSIGEGLKEGKTMDTINLIKEIAKEQSTVNLLTAIGNALKSEKVKPIGLGGLLSSLRDPQVQKGLGVVIEILRNLGSYYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P9</accession>
    <name>F4B6P9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <source>
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        </source>
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    </evidence>
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    <sequence length="88" mass="9920" checksum="6BEF5A325BEEF364" modified="2011-06-28" version="1">MYEIKVVLESIRDGAVNPGEVVIRTKIPRYEVLAIFHILEGLGLIETIYSKGSHKVYKLTQKGEEILDALEKGHEIDIITKESKDALI</sequence>
</entry>
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                <person name="Shah S.A."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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        <source>
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        </source>
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        <location>
            <begin position="10"/>
            <end position="61"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
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            <dbReference type="EMBL" id="AEE94615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="74" mass="8806" checksum="28356487AAEE1E68" modified="2011-06-28" version="1">MSVVLSVRVRKELKEKAEQLGINIRDVVEKALEEAIKEKEKEEINDIARKIKELMKDVSEEEWVEAIREERNER</sequence>
</entry>
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    <accession>F4B6Z1</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        </source>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AEE94684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="71" mass="8079" checksum="972555012EFAFD80" modified="2011-06-28" version="1">MEELIKKAEEEGIDVEDIIINAIRNESEDPSISIKLRIEIAEKYINEAKKYLENGDIIQASEKAYKCLPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z2</accession>
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        <submittedName>
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        <name evidence="3" type="ordered locus">Ahos_1810</name>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="GeneID" id="10601310"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <location>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94685.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="441" mass="51927" checksum="80E3EE1F9BCDB0F3" modified="2011-06-28" version="1">MLKTLKRTVRVESDFLNEWKYRVLREVEEYQRKIVNEMIEVILSEALPTTRKKLHERFYNHYKEKYPFLPSRVIEGAYIVAGRIVKSFKERKKEGLTRKDKPEYKKVLITIPNMVNWRFNRVFISVLTHKGWVEVPLKFTKQFIHYLYGGWRVSQELKLRLIGRKVLVWLTFEKEVEVETKDGNYISIDVNENNVTLAVFENFKLKELRRYETGLGRIVVNYSIRREEITKGHSTKDELIRKKLRKLREKERKIDVLRKTVKRITELARSLSAKVVVGKFSSKVKEKMESNKNDKLRHRIHQWSVVKFVDMLKTQPIEVDEVSESYTSSTDPFDGKKLRKRKQIVERVVKVFNPHLMTGPAHEGGGVKVLKMNARYLESGQVLLERDSIAPLNLVRKVDGRVLVFPSTSPKDLRVTVYDPLRGVPVAELEVIKSKEKLRHG</sequence>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
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    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="AlphaFoldDB" id="F4B724"/>
    <dbReference type="STRING" id="933801.Ahos_0619"/>
    <dbReference type="GeneID" id="10600066"/>
    <dbReference type="KEGG" id="aho:Ahos_0619"/>
    <dbReference type="eggNOG" id="arCOG02048">
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    <dbReference type="HOGENOM" id="CLU_1253635_0_0_2"/>
    <dbReference type="OrthoDB" id="36223at2157"/>
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        <property type="term" value="P:tetrapyrrole biosynthetic process"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="HemD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrapyrrole biosynthesis uroporphyrinogen III synthase" evidence="1">
        <location>
            <begin position="40"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="220" mass="24870" checksum="ECD23CF35D9FABC4" modified="2011-06-28" version="1">MRILFLRPEGSNIPKVDFAEVINIPIFSPICIDYPSLPLTEAYAFTSSNAVKCFHDFDKIKGKQIFSIGKSTAKELMAHGINSLYPPNYDSLDLAEFIKSKGIKSLTAIRSKKASNDLKNSLQNIEYHEIYDYDTIINKEELEKSKDYLENCKIDVVVLTSSEIAKAVASSLKNCYKIVSIGPMTTKTILSLRPDLKIFQSKIYDIEGVIDLIKREVKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B728</accession>
    <name>F4B728_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 13">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="3 8 13">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemA</name>
        <name evidence="17" type="ordered locus">Ahos_0623</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8 13">
            <text>(S)-4-amino-5-oxopentanoate + tRNA(Glu) + NADP(+) = L-glutamyl-tRNA(Glu) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 13">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="3 8 13"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775427.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B728"/>
    <dbReference type="STRING" id="933801.Ahos_0623"/>
    <dbReference type="GeneID" id="10600070"/>
    <dbReference type="KEGG" id="aho:Ahos_0623"/>
    <dbReference type="eggNOG" id="arCOG01036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035113_0_0_2"/>
    <dbReference type="OrthoDB" id="4562at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019353">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.30:FF:000001">
        <property type="entry name" value="Glutamyl-tRNA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="entry name" value="Glutamyl-tRNA reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000752">
        <property type="entry name" value="PRK00045.4-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="Glutamyl tRNA-reductase catalytic, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl-tRNA reductase N-terminal" evidence="16">
        <location>
            <begin position="27"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="15">
        <location>
            <begin position="170"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrapyrrole biosynthesis glutamyl-tRNA reductase dimerisation" evidence="14">
        <location>
            <begin position="296"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="187"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="8 12">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE93511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="419" mass="47986" checksum="16E7EB2283BB3744" modified="2011-06-28" version="1">MSILNEIENRYFAIIYTYKTIGFDNLSSHYLRENEISLIHNIVGSQMAIIQTCNRIEIYLYSEDSDTTTKLLNFLDQVHGKKISIDATILTGSKAIEHLFEVASGIDSMAIGEYEILSQVRLSLENAKKLHMIGKELEILFERAIKVGRRVRQQTNISKGKVGLYSIAIELAKTRVDLKTSNIAVIGAGQIGSRLISMLNSEGAKNVTILNRTVEKAKELAEKYGYSYLSLDFSKLDGFDVIFSAIFYPRVIRIDNKFIIDLSSPPIFQGNEVYTLKNLQQISEEIRKKRLSELSKAKDIIEEGIRDFILDYENLKYDMIVSQIMKNIEEIRQNEVKRALKELNGDNAEEVLNAMTKSMVKKMFYPLLSKIKEAVRNNETNYINLILELFNNGKLSDSKTKEIEEKQVNKRYDSRNQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B738</accession>
    <name>F4B738_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl40e</name>
        <name evidence="5" type="ordered locus">Ahos_0634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775437.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B738"/>
    <dbReference type="STRING" id="933801.Ahos_0634"/>
    <dbReference type="GeneID" id="10600081"/>
    <dbReference type="KEGG" id="aho:Ahos_0634"/>
    <dbReference type="eggNOG" id="arCOG04049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175093_1_0_2"/>
    <dbReference type="OrthoDB" id="45138at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_eL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_eL40_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
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        <property type="entry name" value="PRK04136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="50S RIBOSOMAL PROTEIN L40E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL40" evidence="4">
        <location>
            <begin position="3"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="56" mass="6547" checksum="3A8EC37B8903E4B9" modified="2011-06-28" version="1">MPITDPEKLRIVQERVFIKKVCRNCGALNSIRATKCRRCHSRNLRIKKKELPAKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B782</accession>
    <name>F4B782_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776617.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B782"/>
    <dbReference type="GeneID" id="10601327"/>
    <dbReference type="KEGG" id="aho:Ahos_1827"/>
    <dbReference type="eggNOG" id="arCOG01204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_0_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041633">
        <property type="entry name" value="Polbeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18765">
        <property type="entry name" value="Polbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase beta nucleotidyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="13747" checksum="B1D95655794501C2" modified="2011-06-28" version="1">MKLIMLFGSRARGDFTNSSDYDVLVVGDEIPKDPRKVPNELYLKVMKMFPGEVDAVFMNSEVFLKKLREGSPFFLQIIEEGKIIEKDEEFWREVIKIYNKLRPLYERKGKAWIRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B795</accession>
    <name>F4B795_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776630.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B795"/>
    <dbReference type="STRING" id="933801.Ahos_1842"/>
    <dbReference type="GeneID" id="10601341"/>
    <dbReference type="KEGG" id="aho:Ahos_1842"/>
    <dbReference type="eggNOG" id="arCOG04291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192595_0_0_2"/>
    <dbReference type="OrthoDB" id="35747at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27826" checksum="909AEF19220B0440" modified="2011-06-28" version="1">MIKVGTCGFTNKHFKYFDVLEVQQTFYDVVSETTLSRWRKMAEENRVELTLKALQVITHEYNTTTYKRMKKFQGNKENFGSFKETKEVEEATEVTLKEAKELNAKIIVFQSPASFKPSEENVKRLIDYFSTLKKGFIYAWEPRGSWYDNESILKEVLEKTGVIHVVDPFRHNSLTQMKYYRLHGIGKGEVNYSYKFTDEDLQKLKDMVKGSSPTTIYVLFNNIYSFDDALRFKKMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B798</accession>
    <name>F4B798_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B798"/>
    <dbReference type="STRING" id="933801.Ahos_1845"/>
    <dbReference type="KEGG" id="aho:Ahos_1845"/>
    <dbReference type="eggNOG" id="arCOG05341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2490236_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9430" checksum="E40C5C88E33B7AE4" modified="2011-06-28" version="1">MREIFPVFLVYAFSAFFAFFGVWISELYPIQVRATGANFTLLIGRLIGGGFGTFIVSLIPLQLNVSLSLDLIITAIVATIGISLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A7</accession>
    <name>F4B7A7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">High-affinity zinc uptake ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A7"/>
    <dbReference type="STRING" id="933801.Ahos_1854"/>
    <dbReference type="KEGG" id="aho:Ahos_1854"/>
    <dbReference type="eggNOG" id="arCOG05372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987485_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43850:SF2">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN MA_4021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="5"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="13651" checksum="1F577A6677F8AA35" modified="2011-06-28" version="1">MKVDGEKVLLLGPNGSGKSTLLSAIAGIIPYKGSIRIDGMEVRNIKNYNAVATNLPQAFSIGLTIDDVIEIYDELKGLKINVKAELEKLGIKTNNRIYQLSAGQGVNHFFSTHDLISCHLLNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D1</accession>
    <name>F4B7D1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peroxiredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776666.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7D1"/>
    <dbReference type="STRING" id="933801.Ahos_1878"/>
    <dbReference type="GeneID" id="10601377"/>
    <dbReference type="KEGG" id="aho:Ahos_1878"/>
    <dbReference type="eggNOG" id="arCOG02064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094970_2_0_2"/>
    <dbReference type="OrthoDB" id="288304at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF2">
        <property type="entry name" value="PEROXIREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17101" checksum="CB78A8A10B0E02B2" modified="2011-06-28" version="1">MESKRKLGLVFASGASNRICCVAIYGGAALAAGWEVIVHLVNEGVVAFRKDVLPKLNTLGLEMTIKPEFYTPYTDQFLKNVENAVKSGQLKDWYSFLKQLKMDYKDSFRIYACPVASAFYNFKKEDLVDIVDDIKGAETFLEEVYGGVVMYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D6</accession>
    <name>F4B7D6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdopterin dinucleotide-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1883</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776671.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7D6"/>
    <dbReference type="STRING" id="933801.Ahos_1883"/>
    <dbReference type="GeneID" id="10601382"/>
    <dbReference type="KEGG" id="aho:Ahos_1883"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_281836_0_0_2"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF10">
        <property type="entry name" value="NADH-QUINONE OXIDOREDUCTASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin dinucleotide-binding" evidence="4">
        <location>
            <begin position="997"/>
            <end position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1131" mass="125738" checksum="C48F18AB8BA17E63" modified="2011-06-28" version="1">MSSSSTQYTGVPNSKVPLPPTSAERYFATCRFCNVGCGYDIFVFPVGQEGQPKAGQNGIQYNFIDYVFTVNGKPRNLQGYQADYTSPLQPLSALTGTPWIGEGMVTRTMRYNPTKQSWEEVWIEEIPSPECPVNQGNYSTRGGRNAQRNWSPFSDVASGFAVYQSRIKTPLIRWNGSLQPIGWSYAIKVAAKILKYYMDNETFEYPVGPGAVQVLAIRADHGGGQGGGVFSNLMPGLLLHMGLATPFVRFHYQVAFSFTQDALMEATNGNGTDTASMLDITIADNLILWGINEYTTSTVNLIQHIFDNITGATIDRKQSWFESGEPALPANIIIVEARPSETVKAVTAKLGVSVQDAMQGYDVTAGAVYNGQYNPSHQYVVFAQVNPGTDAELANAIAAYIYYTYPDVVNHFITMYQNASQYGFQFNQQTYQYYIQYLQSKSLDEWLSEASQITGVPQSVIQLIGDIIAAPKTNSSGQSFYKRTLIEFEKGVIWSGNYTPIYAIANLGIISGALSGRPGCGVSTGFGHQRGAAFPAPPPPPWSSNLNAPRNYWIQMHEYVPQQLQAQGQAVTGTAIAEFIKNFYNQYTSTLVPQVYQDNPVIDYLIYSGYGKVLWVFTANPYKLTMSGEKLSQVIDNRAHLLQECVENTINLGIPSSNTDSQILSGQYNTQNVGAPLPNFPDPDTYANAVISCLSGSNAVPGALFVVGNDIILQQNGVLERAAHLLLPSASNHGETYEIRWDGHDRRLRLDEVYHSPPGSAMPDVWIYAMIAYEIYQLYQAEGNQNSPQAQRIYQAFNQIWNSLSAHMIQNTQPLSLISSYSEFYYDYDWFNIYNDLWNYYVANGPTNFKSWPYGYIVPHWAPGWAQIDLNDLKAARTIGVQLPILGKTTNSDGSFTLYGLVNYAEPAISGKLRVEEVTINPNQAITVGTTPSGQTITLPLITRQVEYISIDDLQSMFGYSWNDLLPYVINGFNPFPTPYVGVFGYAAQLQSKYKYWLNNGRWNIIFQSGWIDYQIPEILQRLPYPIVMMSSTDAQNEGLQNGDIVELYNDYGSVDAIVWISDTVPPGQLFLAMAFEVKPGANNATTPTVDPVTDNQMVKWSWVNIQKIGTLSEETKARLTFAPVSFHLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7E8</accession>
    <name>F4B7E8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YjbQ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7E8"/>
    <dbReference type="STRING" id="933801.Ahos_0666"/>
    <dbReference type="KEGG" id="aho:Ahos_0666"/>
    <dbReference type="eggNOG" id="arCOG04214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15308" checksum="EA997D7CA7E052A7" modified="2011-06-28" version="1">MHMKIFEKEFKVKTNERFESIDITDNVEDAAKSIDSGIAYIFVKHTTCAVIVNEAEKGLMSDYLEWAKKLVPPEGKFNHNIIDNNGHAHIISSIIGNSRVVPVKGGKLDLGTWQRIILLEFDGPRVRTVNVKVMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M6</accession>
    <name>F4B7M6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776688.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M6"/>
    <dbReference type="GeneID" id="10601399"/>
    <dbReference type="KEGG" id="aho:Ahos_1900"/>
    <dbReference type="eggNOG" id="arCOG08554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2379215_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10370" checksum="8D1C6678AD6C389F" modified="2011-06-28" version="1">MKKIAIITLTLIILAIISFIQLYPLANVFFTSLFPGNNINLIFLSKSGKPISHVTVYAYTFYSTCRGTKFVIVINATATSCTIPEKDLLIPAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R7</accession>
    <name>F4B7R7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Peptidase M1, membrane alanine aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1941</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776729.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R7"/>
    <dbReference type="STRING" id="933801.Ahos_1941"/>
    <dbReference type="GeneID" id="10601440"/>
    <dbReference type="KEGG" id="aho:Ahos_1941"/>
    <dbReference type="eggNOG" id="arCOG02969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014298_0_1_2"/>
    <dbReference type="OrthoDB" id="139771at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09603">
        <property type="entry name" value="M1_APN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="10">
        <location>
            <begin position="28"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="9">
        <location>
            <begin position="215"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="735" mass="85953" checksum="03C94AAF5FC08153" modified="2011-06-28" version="1">MVDRVGKNYVLKEHEIHYPENYDFRILHYIYYLEIYPEESRIKGKAVVKFLGKDLILHARHFQINKIKLDGKEANYSYDGEKISIQREGKEAEIEYEAKPVLGLIFKKLKEYIEVATTGEVDQARNWIPILDDPRVKTTTEFYIKVRKPYIALSNGRLIDKEEDGDFITFHWLMDKPHSPYLNSIAVGMFSKEEDEWEGVKLENYLPKGYEEFFWNLSSVKKAIEFFSSYTGVKYPYCRYAQVVLFSMNGGMEYITSTHLTWRVIHDKVAEKNYSSENLIAHELAHQWFGDLITTKDWPNIWLNEGFASYFEALYVEHEKGREEFLYNLYNKWKGYEEEKEKYTRPIVCRYYKWGDELFDRHTYNKGALFLHKLRNFLGDETFRKGIKEYLERFKYRSVDTEDFKKVMEEVSGKDLTFLFDQFVYSAFNPSVEVTSIDGGVRIKNEVEVDVKIGKEIRKVKPGESLLSGAYVCVDPNFTSLAEVEDKQGEEALIKESKDEELICRIRAVTSLSRFSDFKAIQALKERLENDFWGVSYESAISLGKIKNDDALNALMLNHENPKVMVGIAKALGEFKFNQKAKNFLLEVMDKAESYHVRAEAIVSLGKIGFTDVKEKIISHFEDESYADVVPSAVIEALSYFGDDESFRFVIDRGFKNEKETIRAAFARNLWRFGDKSREYLYYLVKDPSPIVRGATIKSLEELKMKDLLRTIVDNEDEELRNRSNALRVLYKLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W2</accession>
    <name>F4B7W2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl24e</name>
        <name evidence="12" type="ordered locus">Ahos_0753</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775555.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W2"/>
    <dbReference type="STRING" id="933801.Ahos_0753"/>
    <dbReference type="GeneID" id="32173742"/>
    <dbReference type="KEGG" id="aho:Ahos_0753"/>
    <dbReference type="eggNOG" id="arCOG01950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190191_0_0_2"/>
    <dbReference type="OrthoDB" id="55506at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055345">
        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034186">
        <property type="entry name" value="PRK14891.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="11">
        <location>
            <begin position="8"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="62" mass="7407" checksum="6F6C3D461BAA076E" modified="2011-06-28" version="1">MPPVTHKCSFCGKEIRPGTGLMYVMNDGTILWFCSSKCRKYMLKYHKDPRKLKWTTSYMRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B846</accession>
    <name>F4B846_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776785.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B846"/>
    <dbReference type="STRING" id="933801.Ahos_1998"/>
    <dbReference type="GeneID" id="10601497"/>
    <dbReference type="KEGG" id="aho:Ahos_1998"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922651_0_0_2"/>
    <dbReference type="OrthoDB" id="39912at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="14819" checksum="3C6CCA3159C191C7" modified="2011-06-28" version="1">MSSFDNATLLCKRALNYLRTAKDSFDKGIYDASATNCQISAELPIKSTYLLLGVNFPQTYSIRKLLSGLAMAAGNYSEEIQRFIKEKRKEPNLVELSRFGVQYSLVDVDAEYASDCLNFVEDYILPLIRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B872</accession>
    <name>F4B872_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776811.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B872"/>
    <dbReference type="STRING" id="933801.Ahos_2024"/>
    <dbReference type="GeneID" id="10601523"/>
    <dbReference type="KEGG" id="aho:Ahos_2024"/>
    <dbReference type="eggNOG" id="arCOG08324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559380_0_0_2"/>
    <dbReference type="OrthoDB" id="43088at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="19622" checksum="3B568FA0A3FC5D45" modified="2011-06-28" version="1">MIRGILLSIGITLISLSLLSITSPISNTIIVTKPYYISIPSTAKVIAIMYENSTNVTVYVKIIHGNFTKIIRPPCTIMLTHGKWIFEVYNETYPKISYRSINETIIEKNVTIIIQKTVNYTNIVTTNNATYPIYVILYIKCMKILKFQELSEILGIIMIISSVFLYLRKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D1</accession>
    <name>F4B8D1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl3</name>
        <name evidence="8" type="ordered locus">Ahos_0844</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D1"/>
    <dbReference type="STRING" id="933801.Ahos_0844"/>
    <dbReference type="KEGG" id="aho:Ahos_0844"/>
    <dbReference type="eggNOG" id="arCOG04070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_uL3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_uL3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003261">
        <property type="entry name" value="PRK04231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="254"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="361" mass="40665" checksum="76233D4CA9A7C388" modified="2011-06-28" version="1">MQSWNSYFYNLIVVHEIRRGRGLGHRKLASPRRGSSGFRPRKRAEELLPTPRSWPRISSSNPILLGFVGYKVGMTHVYYINDIKGSSEYGKEVFTPVTVIETPPIIPIALRAYILGSKGEPEVLTDYWTPELPQEITRKIKSLKVNKDKLNEFLDKIKSNLNNILYLRAIIATQPKLVPALGKKKPEIVEIQVGGGDTSAQLNYLLDILGKPVNVTDIFKEGQLVDIIGVTKGKGFQGVIKRYSVMELPRWHKHRKGSRKVGTKGPSLGTPSYVPQPGQLGFHRRTEYNKRILKISTNPQEINPAGGFVKYGLVKNPYILIQGSTIGVRKRPLFLRYPIRPYEVPAEAPKITYIDLSSKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H5</accession>
    <name>F4B8H5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase, GlpC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H5"/>
    <dbReference type="STRING" id="933801.Ahos_2056"/>
    <dbReference type="KEGG" id="aho:Ahos_2056"/>
    <dbReference type="eggNOG" id="arCOG00332">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF2">
        <property type="entry name" value="HETERODISULFIDE REDUCTASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="1">
        <location>
            <begin position="191"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="291" mass="33068" checksum="E6BE49CB749362AC" modified="2011-06-28" version="1">MNNLSEIGKILVDNLNRYYMPFPVDKKVCSEWAKDLPKGGNTIIYTSCMYQIEPAVGMFLKFLPSISSFKGLMPLARLVKPSRSQLDRAYKILNNIVKALEKSGIKPGYLYEEEPYSGAILLEMGFLEEFGEYAKKVVSLFKEKGVRKIITVDPHTHNALTRYQEFVKHDLEIVNYLELVNYTKKVEGEFTIHDSCLYSRFLGLREKYRGLIEKSGIKLVEDEMITGKDTSSCCGGPLGPVDLKVSEKIAENRAKDLEKLSKKLLVVCPICYVTLLPHFKGEIMDIAEVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N9</accession>
    <name>F4B8N9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type anion transport system, duplicated permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776906.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N9"/>
    <dbReference type="STRING" id="933801.Ahos_2120"/>
    <dbReference type="GeneID" id="10601621"/>
    <dbReference type="KEGG" id="aho:Ahos_2120"/>
    <dbReference type="eggNOG" id="arCOG00174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036171_2_0_2"/>
    <dbReference type="OrthoDB" id="50379at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42744">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42744:SF1">
        <property type="entry name" value="BINDING-PROTEIN-DEPENDENT TRANSPORT SYSTEMS INNER MEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="11"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="324"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="544" mass="61666" checksum="90C777CA26EDF246" modified="2011-06-28" version="1">MFDIWLAIYDTLLTLGRVFIDIAFAIVTGWLLGYISLKSKTFENIYISLSEIFESVPVISFFPVVLIFFVFSIGGSLGIELAVLFLVFTAVVWNIWMGIYQAFKTVPSEMEEVIRNYRFGFVGKMLRLYIPFSIPRIAANLIPSFADALFYITVSEVFSIGTKCYEVPGIGTLIAQYTAEGDYTDAIYALIVLGIFTTTITLLLREFAEYSVKKYGLDTEASLKSFKRGRFRIRYSARLSNSKGVFAKLGKYLTKPPYFTRQNRLMDEEEEAKKRKIPINMISAGIGILLLIIILYGAYSVIISVKPSTWSYLISTLPQDLIDLGADYLRVAVISLMSFIFAVFVGYYIARHTNADRAIVPIIQTFSAFPAPAYFPLLYGFTYTYVSSIFGPFTNEFYVLLLGFISTFYYVFYSFWIGVKNLPSEYWELMDNLKLGFWTKMRKIILPATFPYIIAGMSSTINSAWGGLAIGEYWPDIYQSQTLEVRTGLMKALAVADNQGNLALVGWLSLIFAIIVVIYSVLFTRKMMDLARKKYVAEEGIYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8P5</accession>
    <name>F4B8P5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776912.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8P5"/>
    <dbReference type="STRING" id="933801.Ahos_2126"/>
    <dbReference type="GeneID" id="10601627"/>
    <dbReference type="KEGG" id="aho:Ahos_2126"/>
    <dbReference type="eggNOG" id="arCOG07281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_846275_0_0_2"/>
    <dbReference type="OrthoDB" id="43684at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="299" mass="34656" checksum="BA70C3952499D9F5" modified="2011-06-28" version="1">MLDIEKAYKLYSDYKKTGNRELLSKIEELIKGDSSIQGLNLLALVYIEKGKYNDAINALDIALKKVKDDEDKATLLFNKALALFRKGDLDSSYEILKSIPSRTSVYAHSRRFLAQVCIKKGDLRHLEEARQILESFDIPNEDLAVTYILLSRLKDKSLYKKAVEIAKSIKNEKLLAEALLSSDDEKELEEALEIFRKLNDVNGEAKVLYKLSLKKPELLYEAIQRLEESGESRERQTLLYELYKRTGILNFLKEAIKIAEKYNDYLFLARSYVELSKKENELENLRKAVLFYEKYVESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q7</accession>
    <name>F4B8Q7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Small ribosomal subunit protein eS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps8e</name>
        <name evidence="7" type="ordered locus">Ahos_0891</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775693.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q7"/>
    <dbReference type="STRING" id="933801.Ahos_0891"/>
    <dbReference type="GeneID" id="10600362"/>
    <dbReference type="KEGG" id="aho:Ahos_0891"/>
    <dbReference type="eggNOG" id="arCOG04154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="OrthoDB" id="372305at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11382">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000002">
        <property type="entry name" value="30S ribosomal protein S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_eS8_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="14524" checksum="06C29114EDF5986A" modified="2011-06-28" version="1">MGVYQGNDLRKITGGLKGRHRDKRKFEMGELPTETKLHTEDIREKVRVMGGNFKIKLKYAAFANVYNPSDHTYKKVKILEVEEVPSNREYARRGIVVKGARIRTEIGEAIVTSRPGQDGVINAILVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R6</accession>
    <name>F4B8R6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">Triosephosphate isomerase</fullName>
            <shortName evidence="7">TIM</shortName>
            <shortName evidence="7">TPI</shortName>
            <ecNumber evidence="7">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tpiA</name>
        <name evidence="8" type="ordered locus">Ahos_0900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775702.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R6"/>
    <dbReference type="STRING" id="933801.Ahos_0900"/>
    <dbReference type="GeneID" id="10600372"/>
    <dbReference type="KEGG" id="aho:Ahos_0900"/>
    <dbReference type="eggNOG" id="arCOG01087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="OrthoDB" id="9465at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000223">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003302">
        <property type="entry name" value="PRK04302.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Electrophile" evidence="7">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="25098" checksum="B47A5381AAA6B734" modified="2011-06-28" version="1">MKKPIILINFKAYENSFGKKGVDIAKRIEKISIEYSTEVILSVPATMIPILSQEVSLPIYAQHVDSYPLGAHTGSLLPEMIKEAGAKGTLLNHSEKRIRADEIHDALLRLNKLGLESVVCVDRYELVEPMAILRPTAILIEPPELIGSGISVSKAKPEVITNAVKEISKVPGVFLIAGAGISNGEDVYTAVKLGADGIGVASAVMKAKEPEKVVEDFIKSGIKAMDERNDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T7</accession>
    <name>F4B8T7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Prangishvili D."/>
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                <person name="Prangishvili D."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-asparagine + H2O = L-aspartate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="EC" id="3.5.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776936.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z8"/>
    <dbReference type="STRING" id="933801.Ahos_2150"/>
    <dbReference type="GeneID" id="10601653"/>
    <dbReference type="KEGG" id="aho:Ahos_2150"/>
    <dbReference type="eggNOG" id="arCOG04779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021603_1_2_2"/>
    <dbReference type="OrthoDB" id="18230at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14950">
        <property type="entry name" value="Asparaginase_2_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="164"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="186"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="135"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE95021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="271" mass="28600" checksum="4A26E37F24EF0F50" modified="2011-06-28" version="1">MKYQKPTLLIHGGAGNWKERDSEKAIKEINNALDRGFEEFKRGSSLEAVVEAISYMEDSGVFDAGRGSVKNSKGEIEMDAGLMYGNKLSVGAVAAVKVKNPIRLALQVMKDGRHVLLVGKEAEEIFPSTPLSVEDTVGAVALDIDGNLVAGTSTGGIANKLPGRVGDSPIPGAGYYATSRVAVSSTGIGEIILKILPAKEVDILVSMGLSICDAVRSVINKVTNIFGKGNIGMIGIDNNGNATAYYNTVGMARGIKYGNGERKSFVFEGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B906</accession>
    <name>F4B906_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">succinate dehydrogenase</fullName>
            <ecNumber evidence="4">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_2158</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776944.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B906"/>
    <dbReference type="STRING" id="933801.Ahos_2158"/>
    <dbReference type="GeneID" id="10601661"/>
    <dbReference type="KEGG" id="aho:Ahos_2158"/>
    <dbReference type="eggNOG" id="arCOG00571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_2_2"/>
    <dbReference type="OrthoDB" id="23539at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000003">
        <property type="entry name" value="Fumarate reductase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005037">
        <property type="entry name" value="PRK06452.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="12">
        <location>
            <begin position="7"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="13">
        <location>
            <begin position="442"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE95029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="566" mass="62985" checksum="382B9876DA432D44" modified="2011-06-28" version="1">MEKLSYDAIVIGGGLAGLMAAHEIAVSGYKVAVISKVFPTRSHSSSAEGGIAAYIEGNSDPNDNPDYMTYDTIKGGDFLVDQDAAELLSQKSGEIVRIMESWGTLFNRQPDGRVAVRYFGGQTYPRTRFVGDKTGMALLHTLFQRVSGLDIDFYNEWFALDLIRDDKRVVGVVAMEMKSMTPVFFKARAIVMATGGMGMLYAHSTNAYINTGDGYAIALRAGAALKDPEFVQFHPTALYPSDILISEAARGEGAILINNKGERFMARYAPRKLDLAPRDIASRAIITEIREGRGFPGGYVGLDLRHLGEDYIKERLALAYEAAKNFSGVDATQEPIPVRPAQHYYMGGIDVDITGQNPDLIGLFAAGEAACVSVHGANRLGSNSLLETLVFGRETGRAVAKFLSEHKEEDSYSLDKEAEKVVDDAYKFVKSESGVHFGEILNKLRNVMWEKVGIFRDEDGMKDALSQIIELRKMTSSMYVLDKSTTYNTEFFNALELRNMLDLAIVIARTALERKESRGAHYRTDYPERDDKNWLKHTIAYLKGETVEITYKPVRVTRWKPEPRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B909</accession>
    <name>F4B909_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Succinate dehydrogenase subunit D (SdhD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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    </reference>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    <sequence length="65" mass="7596" checksum="E75551B331508DA7" modified="2011-06-28" version="1">MPESLVVRDPKYLGKFIAVDREFNIIGYSDSREELEEELTKKGYSIADYDIVYVPKSLKKDYKSD</sequence>
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    <accession>F4B9C7</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="287" mass="33094" checksum="46CEB1A84F0D8FE9" modified="2011-06-28" version="1">MITVEEIYGEDSWFTYFMRGNEVVHVSQLEGARKVGSISLGNRSLVLWEVPENEITNGYYVNYNPKPRLFRFTRPNILGRDLSFDLMISKTLTPSPLVQLRPRLFGIERTPLFTFRRVAPLEEEIKSSVQKLRLRLPVVNTIEAEIDAMLRPIPQERVRVLNSYSEEIYSQWVLTKVIEAIIEMGGKLIDSFVLNVDSKIVEILYHPNPGVDIHVLVSDKREYVNINNIQSYKDKEVLQIDTGVRTVGKVGITVSFEGNAQFVLTPRNVEGIKKFKEEVRNEIRKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E2</accession>
    <name>F4B9E2_ACIHW</name>
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        <submittedName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="1">Belongs to the methylmalonyl-CoA epimerase family.</text>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95088.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
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    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="GeneID" id="10601727"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="139" mass="15126" checksum="83294F088F9B205A" modified="2011-06-28" version="1">MRYQGSIEVNAKKEVILNFLGNIELVASCFPGIKDIKKDGDVYIVKGTAGIGFIKGDYTAKVKMQRTNDGFKLEAQGNGMNSNVNINANIVVEEGRINYTADVNVSGILASAGARLMEPALNKILNQLFDCIRKKVEVS</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0010945">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CoAse"/>
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        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="157" mass="18039" checksum="5EBDA77E5226FBE6" modified="2011-06-28" version="1">MTCDAAVVLLLNCENKFLLIKRSTNPNDPWSGDMALPGGHREGNEDCETTAKRECKEETGIEPEIIAFLGYYSPHNANITVAAFLGRTCFSKIKRDKKEVAKYFWVSFSELKKGEECFYYKKYRIWGMTYRIIRDYMEKFSPATDTSSFRSVGHSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H2</accession>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H2"/>
    <dbReference type="KEGG" id="aho:Ahos_2247"/>
    <dbReference type="eggNOG" id="arCOG03686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177746_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="109" mass="12503" checksum="8DB5ACA6C17A4E16" modified="2011-06-28" version="1">MNLSSNSSKLTQDKFLFLVKTSDYMEFIINLYGNSENPVVEIHGKKSSVNELYKEGPLYAFLLSIPHCIITMFEHLAKKEGIQVKVCKVRSTYYLDDSLLLFGKYAIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H6</accession>
    <name>F4B9H6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid permease-associated region</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2251</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H6"/>
    <dbReference type="STRING" id="933801.Ahos_2251"/>
    <dbReference type="GeneID" id="10601757"/>
    <dbReference type="KEGG" id="aho:Ahos_2251"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_632542_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_transporter"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="433" mass="47130" checksum="C0FD2ED46B50BE97" modified="2011-06-28" version="1">MNIDKRLEPKKESIPKYLVIAQSLSSIAPLGSVSAYLTFAFQFSLASTFYAAILGALIYLLWVMLGYNYSKIVATTGGIYDFAKTASTETVGKIAGWLYWISYAIYLPSVTTYLTGIVLPSVFNIPPSLLAIIEITIPIILTILLISGIRPPLFYALITSTIEIFLIIILGVKVISITGIKFPQYNIPQYNFWSGALAVAFTLAGGGASFFLGYEAKGRGKTVGSSYLTAFSIASIVVIFASYFETAAVGFGNSGISNLLSVTQYPGFYISQEYLGSTFSTIFFVFTVNSLLGSAIAAYVALSRLTYSLTSRKMLTSIIIVAIFFISFNLIASITGRYCLVYQYTTEASLVTLFSSHAIVSAVYPLFVKKFYKLNLPSVLLGLSSTALMAYGLYSNLIPYEYPNTLIGIVSLISGVILGIVSNILKPILYRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H8</accession>
    <name>F4B9H8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777039.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H8"/>
    <dbReference type="STRING" id="933801.Ahos_2253"/>
    <dbReference type="GeneID" id="10601759"/>
    <dbReference type="KEGG" id="aho:Ahos_2253"/>
    <dbReference type="eggNOG" id="arCOG05379">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682781_0_0_2"/>
    <dbReference type="OrthoDB" id="55804at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018632">
        <property type="entry name" value="AAA-associated_dom_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09821">
        <property type="entry name" value="AAA_assoc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="159" mass="18063" checksum="D67C265E390C2238" modified="2011-06-28" version="1">MYVIDPAARVADLIGLLSTLQGTFGGKTDLYMLEKEMEVDLDDLMPIVYTASYLGFVTVGEGDIIITDKGMEFLNANIRKRKEILRESLVKVEPFITAIELKTFSLESLKEALEKKGIFVYNTPEGDYDLQMTLTEWGVYSGLISRYDDNMYKVNYEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J5</accession>
    <name>F4B9J5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Starch synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777056.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J5"/>
    <dbReference type="STRING" id="933801.Ahos_2270"/>
    <dbReference type="GeneID" id="10601776"/>
    <dbReference type="KEGG" id="aho:Ahos_2270"/>
    <dbReference type="eggNOG" id="arCOG01420">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_18_5_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="4">
        <location>
            <begin position="15"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="343"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="566" mass="64477" checksum="310D605EC872B404" modified="2011-06-28" version="1">MKRIESLWIPDKIEKIWLLTFELSKIASMGGLGTAVYNLGKELASLGKQVIVIMPSHGRHMNDYYRSLLKLRDSGITIYGIRKGMSGAYYPYKLGFEIGELDGMKIMLVKGLDYDTGRIMDSWGIYDYAMEKSALLARAIEGLIQNMSLDDVPSIIHVNDWHSVLAGVKAKLMFEQRRVIVPLMYTIHLLNKVGAPWHYASPDWGGLEDYYHYIWMVAKHIAYKTSVLWDYCEGKIERFGAYEADIIATVSKSYLNYDVFPYIGNFMENKSCVTYNGTDWKLEEASALSIKYFGTADRKVAKKSAYKLISLQKAIPEDYTTGNILWNNRHRVGIKDDWTTEELQDGPLVLFTGRIIYQKGIDLLLRAFKDVVNEIWNARLIVFGVPTGDYGLLQDIIDRSAEIRDNVRLLISHGINPEIYKVMHYVASVFVVPSRWEPFGINAIEAMAVGTPVIAYSVGGLTESILDLRWNKDGTGFLVEPESISSLAAAIKTAIYLSLADENEDRSYLNKSYIYKTDNVHIWSVVRENAVKRVEENFRWNKVANSVLNCYEKGLIMAKYRALAYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J8</accession>
    <name>F4B9J8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777059.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J8"/>
    <dbReference type="STRING" id="933801.Ahos_2273"/>
    <dbReference type="GeneID" id="10601779"/>
    <dbReference type="KEGG" id="aho:Ahos_2273"/>
    <dbReference type="eggNOG" id="arCOG03285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028187_0_0_2"/>
    <dbReference type="OrthoDB" id="36362at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF13">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="1">
        <location>
            <begin position="272"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="621" mass="71802" checksum="9E3CEAE81F3023F2" modified="2011-06-28" version="1">MRYASIGNGRLLVNLDEFGRIVDFYFPYIGMENQTAGTPIRYSFWVDNKAILDTDLITSLSYLDDSNIIQIESKKDWLSISSFAFIDPDSPTYYLIMKILNNSGENKRIKIFFTHDFNIYSNPFGDTAFFDPYTSSIIHYKSKRYIGIKLLSAGTFDVEYNTTKGNPLDDIYDGKLDQNPIAHGNVQSSIGMEIKVNSSSSSKIYYVITAERNLDDLRKKLTKLNTAEIESNFVSTYMFWKSWVNKGSKERNSLNKLYNVSLFVIKNHMDINGSFIASSDFSFVNLYGDSYQYCWPRDCAYAAYALDISGYGELAVRHFNFIKDLVSPEGFLYHKYNPNKTLASSWHPWIYRGKGIYPIQEDETALEIWAIGSHYERYKDLDELSEIYKKFVKPGLQFIMDFMEDGLPKYSFDLWEERYGIHIYTVATVYGALTKGSILAEGMGDETLAEDSMEVAKTLKDEVKKRMVYNGRFVRRIDENGNKDLTIDASMYAPYFFGMFDSADEVVQNTMELIAQKLNVSNGIIRYENDYYQRRKQLPNPWIITTLWLAEYYIDTGKISEAEKLINWVINRATKSGLLPEQVDPETFESVSVIPLVWSHAEYIIALNKYESIKKKEYDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L5</accession>
    <name>F4B9L5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775847.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L5"/>
    <dbReference type="STRING" id="933801.Ahos_1047"/>
    <dbReference type="GeneID" id="10600521"/>
    <dbReference type="KEGG" id="aho:Ahos_1047"/>
    <dbReference type="eggNOG" id="arCOG07284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150445_0_0_2"/>
    <dbReference type="OrthoDB" id="38300at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="2">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="11952" checksum="DC702BF43AE457D1" modified="2011-06-28" version="1">MIKMKGLIDTGSRYTLLDEEKALYCFNNLDSRYHDLAVINGALTKRYLIKIKLGNSELDVPVSLFSLHSLSLPVVPEIILGREDFLEKFIVTLYKSHFITIYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P2</accession>
    <name>F4B9P2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775874.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P2"/>
    <dbReference type="STRING" id="933801.Ahos_1074"/>
    <dbReference type="GeneID" id="10600548"/>
    <dbReference type="KEGG" id="aho:Ahos_1074"/>
    <dbReference type="eggNOG" id="arCOG11953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200881_0_0_2"/>
    <dbReference type="OrthoDB" id="44104at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="73" mass="8948" checksum="3722C99A49ABD4B0" modified="2011-06-28" version="1">MKIKIWKEWYDILLKLSKDKRTTLEELIKEIMKTKDCINLPRVNTSKKKEINLNLNYTEKEVLERIEKFLFCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R6</accession>
    <name>F4B9R6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenosine monophosphate-protein transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775898.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R6"/>
    <dbReference type="STRING" id="933801.Ahos_1098"/>
    <dbReference type="GeneID" id="10600572"/>
    <dbReference type="KEGG" id="aho:Ahos_1098"/>
    <dbReference type="eggNOG" id="arCOG04298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138382_0_0_2"/>
    <dbReference type="OrthoDB" id="371841at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1520.10">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007153">
        <property type="entry name" value="Adenosine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036902">
        <property type="entry name" value="Ta1353-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36155:SF1">
        <property type="entry name" value="BLL5354 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04008">
        <property type="entry name" value="Adenosine_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103165">
        <property type="entry name" value="Ta1353-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17860" checksum="B6DFE63FF919C37F" modified="2011-06-28" version="1">MVNIDVVRIDIPEGTNVIIGQSHFIKTVEDLYETLASSSPSIKFGIAFNEASGKRLVRFDGNDEELIKLAIENAKRIGAGHTFVIYIKNAYPINVLNRIKNVEEVVRIFAATANPLQVLVAETDQGRGVIGVVDGYTPLGVESEADIKERKEILRKFGYKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S1</accession>
    <name>F4B9S1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxyacyl-CoA dehydrogenase NAD-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1103</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775903.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S1"/>
    <dbReference type="STRING" id="933801.Ahos_1103"/>
    <dbReference type="GeneID" id="10600577"/>
    <dbReference type="KEGG" id="aho:Ahos_1103"/>
    <dbReference type="eggNOG" id="arCOG00251">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_0_1_2"/>
    <dbReference type="OrthoDB" id="51300at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050104">
        <property type="term" value="F:L-gulonate 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF1">
        <property type="entry name" value="LAMBDA-CRYSTALLIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="3">
        <location>
            <begin position="187"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="325" mass="36979" checksum="AA0AB5C19B737292" modified="2011-06-28" version="1">MIKRVSVIGAGLIGSGWATLLATKGYEVSLYTDKKETLDKGIAKIKSYLEVMKNMNLVDKEPDYYMKNIRPTLNMDEAIEGTDFVIEAIIEDYDAKKKVFSYLDEKLDKNVILSSSTSGLLMTEIQKAMKRYPERGVIAHPWNPPHLLPLVEIVPGEKTSQETLNVTKDFMEKLDRVVVVLKKEIPGFLGNRLAFALFREAVYLVDEGVATVEDIDKVMTAAIGLRWAFMGPFLTYHLGGGEGGLEYFFSRGFGYGANEWMYTLAKYDKFPYTGVVKAVQQMKEYSFIKGKSFQELSKWRDEKLFQIYKIVWGDKKQNSNGENNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S4</accession>
    <name>F4B9S4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Carbamoyl phosphate synthase large chain</fullName>
            <ecNumber evidence="19">6.3.4.16</ecNumber>
            <ecNumber evidence="19">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Carbamoyl phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">carB</name>
        <name evidence="23" type="ordered locus">Ahos_1106</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 19">Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 19">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="19">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18 19">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="domain">
        <text evidence="19">The large subunit is composed of 2 ATP-grasp domains that are involved in binding the 2 ATP molecules needed for carbamoyl phosphate synthesis. The N-terminal ATP-grasp domain (referred to as the carboxyphosphate synthetic component) catalyzes the ATP-dependent phosphorylation of hydrogencarbonate to carboxyphosphate and the subsequent nucleophilic attack by ammonia to form a carbamate intermediate. The C-terminal ATP-grasp domain (referred to as the carbamoyl phosphate synthetic component) then catalyzes the phosphorylation of carbamate with the second ATP to form the end product carbamoyl phosphate. The reactive and unstable enzyme intermediates are sequentially channeled from one active site to the next through the interior of the protein over a distance of at least 96 A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="19"/>
    <dbReference type="EC" id="6.3.5.5" evidence="19"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775906.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S4"/>
    <dbReference type="STRING" id="933801.Ahos_1106"/>
    <dbReference type="GeneID" id="10600580"/>
    <dbReference type="KEGG" id="aho:Ahos_1106"/>
    <dbReference type="eggNOG" id="arCOG01594">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_1_3_2"/>
    <dbReference type="OrthoDB" id="85487at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000026">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_A">
        <property type="entry name" value="CPSase_L_chain_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 19" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 19" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="21">
        <location>
            <begin position="130"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="21">
        <location>
            <begin position="670"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="22">
        <location>
            <begin position="925"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain" evidence="19">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain" evidence="19">
        <location>
            <begin position="926"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="706"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="744"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="746"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="751"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="776"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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            <name>ATP</name>
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        <ligand>
            <name>ATP</name>
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        </location>
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        </ligand>
    </feature>
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        </location>
        <ligand>
            <name>Mn(2+)</name>
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            <label>3</label>
        </ligand>
    </feature>
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        <location>
            <position position="830"/>
        </location>
        <ligand>
            <name>ATP</name>
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        <location>
            <position position="830"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
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            <position position="830"/>
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            <name>Mn(2+)</name>
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            <name>Mn(2+)</name>
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        </ligand>
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        <location>
            <position position="832"/>
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        <ligand>
            <name>Mg(2+)</name>
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        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00005077"/>
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        </source>
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        <source>
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            <dbReference type="PROSITE" id="PS50975"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    <sequence length="1045" mass="116696" checksum="6EAE41ED51C2C7C2" modified="2011-06-28" version="1">MVDLKKVLVVGSGPIKVAEAAEFDYSGSQALKALKEEGIQTILVNSNVATVQTSYKMADKIYMLPVTWWAVEKVIEQERPDGIMIGFGGQTALNVGVDLYKRGILQKYGVKVLGTPIEGIEKALSREKFRETMINVNLPVPPSLSAKTEEEAIQKAREIGYPVMVRVSFNLGGRGSTVAWNEETLKRDIGRAFSQSYTHEVLIEKYLYHWKELEYEVMRDSKGNSAVIACIENLDPMGVHTGESTVIAPCQTLDNQEYQDMRTKAELVAKSIDLVGECNVQFALDPNSYTHYIIETNPRMSRSSALASKATGYPLAYVSAKLALGYSLYEVLNKVSGSTCACFEPSLDYVVIKIPRWDLQKFENAEMSLGTEMKSIGEVMSIGRSFEEALQKAVRMLDIGEPGVVGGKVYNSLLTKNELLKYLKERYPYWFLYAAKAFKEGATIDEVYEATGIDKFFLNKIKKLVEFYENLKEVNKDTLIEAKKLGFSDEQIAKKVNKSVEEIRKIRDVEGIKPVVKQIDTLAGEWPAVTNYLYLTYNGSEDDIEFSTNANKLLILGAGGFRIGVSVEFDWSVVSLLDAALKYFDEVAILNYNPETVSTDWDVAKKLYFDELTLERILDIVNKENFRYVATFTGGQIGNNLAKLLEDSGIRLLGTAGHSVDIAEDREKFSKLLDKLGIKQPPWISASSIEEVRKFVGEVGFPLLIRPSYVLSGSSMRIVYNEQELFNFINNLKISTKYPVVISKYLDNAIEAEIDAASDGKGVIGVAIEHIEEAGVHSGDATMSIPTRKLGEGLVKQMKEYALQIAREIEIKGPFNLQFVIKDNTPYIIELNLRASRSMPFSSKAKGVNIIEKALDAIINGLNLDEFYEPPSKAWAVKSPQFSWTQIRGGYPMLGPEMRSTGEAAAFGIDFYDALVKSWLSVTPNKLPEKGKYALVYGIGNEEYLQEAANNLRKYGMEVITLKEVALKGEEEVDSRTAVDLITASKIGIIITNGYLKNIDYHVRREAVDYNVPLVLNGRLGKELTQGFLNSRLTYYEMRDYGGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T7</accession>
    <name>F4B9T7_ACIHW</name>
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        <submittedName>
            <fullName evidence="3">GTP binding protein</fullName>
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    </protein>
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        <name evidence="3" type="ordered locus">Ahos_1119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775919.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T7"/>
    <dbReference type="STRING" id="933801.Ahos_1119"/>
    <dbReference type="GeneID" id="10600593"/>
    <dbReference type="KEGG" id="aho:Ahos_1119"/>
    <dbReference type="eggNOG" id="arCOG00352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="OrthoDB" id="147673at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="159"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="329" mass="37852" checksum="DF2D2911787F2A62" modified="2011-06-28" version="1">MLNPFEKLNCPPKVEDLIKIVLGRLPKISGNTIKDREIRRLIYYDEQVKRYLNFVNSFPRIDNLHPFYKEALEITAGKSIDNIKICLSIINKTSLTASSILKRYINEIRKSDETLANKLMRQAFGRVSSLLRKRKDCIDWIIDLTKNMKKMKSIDPEMPTVIIAGSPNVGKSTLVSKISSAKPEIANYPFTTKEIHVGHFDINGIKVQVIDTPGILDRPMKERNQIERKAINAIKNLNGIIVFLFDISQQSLYSPKEQFDLLREILEFNKNAIIALNKIDSKDEKLYAEVSKMLRENSLNFMEISAENNIGIDDLKEEIKKRVLEMVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W5</accession>
    <name>F4B9W5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="10">ITPase/XTPase</shortName>
            <ecNumber evidence="10">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_2310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>ITP + H2O = IDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XTP + H2O = XDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the YjjX NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777096.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W5"/>
    <dbReference type="STRING" id="933801.Ahos_2310"/>
    <dbReference type="GeneID" id="10601818"/>
    <dbReference type="KEGG" id="aho:Ahos_2310"/>
    <dbReference type="eggNOG" id="arCOG01221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087417_0_0_2"/>
    <dbReference type="OrthoDB" id="52857at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000002">
        <property type="entry name" value="Inosine/xanthosine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050299">
        <property type="entry name" value="YjjX_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699:SF2">
        <property type="entry name" value="NON-CANONICAL PURINE NTP PHOSPHATASE_PRRC1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-canonical purine NTP phosphatase/PRRC1" evidence="11">
        <location>
            <begin position="6"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE95181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="169" mass="18532" checksum="A82AE0F28978D9BB" modified="2011-06-28" version="1">MLVSVGSKNRTKVEAVKEALNIIGLKAEIIPVEVDPGVSSQPYCKETLFGARNRAIRALITANADIGIGIEGGICFEYNKVLAFAVVYAVDKEGNENFSKSASFTLPPKVVKYINQGIELGYAVDKVYSRSGTKYSEGAVGILTKYIDRKRLYVDPVILALYPFYNKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X6</accession>
    <name>F4B9X6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2321</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777107.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X6"/>
    <dbReference type="STRING" id="933801.Ahos_2321"/>
    <dbReference type="GeneID" id="10601829"/>
    <dbReference type="KEGG" id="aho:Ahos_2321"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059688_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43271">
        <property type="entry name" value="BLL2771 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43271:SF2">
        <property type="entry name" value="BLL2771 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="356" mass="38664" checksum="6358F0B0DF26B795" modified="2011-06-28" version="1">MAMKGYSIFLARIIYAISWFYLAPAIPYIILTFKVPLNLAGLIPFSFFLGSGSMQIPSAFISSKLGSRNTLVLGLFIMSLSVFLIGVSKVFYQILVFYFIAGVGASMFFSTGAAILAYLNEKKVSTALGVYNSMFALGGIIGLNWIILDEKFGFSISSVILSVLTILAAVININKPNLKMNWKIIKDKNSFIIGVASAGVWGIYYVVGELFPSFAKFYLGIQILQSSEFTSILLFSSMFGGLLGFLGDRMDKIKLLIISSLLGSIPALLLYTKYYILGIIIMGLFNELSISIVYAVASIGKGINSGISLAEVNSLNILIGSLFEPIGSLVGSMIWVVTTILSLIPLILLKKLKVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z2</accession>
    <name>F4B9Z2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nudix hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2337</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777123.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z2"/>
    <dbReference type="STRING" id="933801.Ahos_2337"/>
    <dbReference type="GeneID" id="10601845"/>
    <dbReference type="KEGG" id="aho:Ahos_2337"/>
    <dbReference type="eggNOG" id="arCOG01073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_5_2_2"/>
    <dbReference type="OrthoDB" id="104705at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03424">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5_UGPPase_Nudt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF18">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="19865" checksum="D52A878E790143E0" modified="2011-06-28" version="1">MKLFSGKKFEVYLDKFDLPNGKIRETEYIKHRGSAVIIPLIDENTIILEKQFRPIIGKWIYELPAGTMEEGEIPETTAKRELIEETGYEAETLIHLIDFYPSPGVSTELMHLFLAKGLKFVGSKPEEYEVIEIEKKNIDEILNMIKNHEIEDAKTIIGILYYQFMLRNQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA10</accession>
    <name>F4BA10_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775923.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA10"/>
    <dbReference type="STRING" id="933801.Ahos_1123"/>
    <dbReference type="GeneID" id="10600597"/>
    <dbReference type="KEGG" id="aho:Ahos_1123"/>
    <dbReference type="eggNOG" id="arCOG00731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153620_1_0_2"/>
    <dbReference type="OrthoDB" id="35765at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38600:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="101" mass="12033" checksum="C701184B4E562139" modified="2011-06-28" version="1">MSERERKEVKKLFKFLFFTSRGGLTRLRIIRLLEDSSLNANQISTLLRMDYKTIIHHLEVLTQNGIIIKDGEKYGAVYRLTSFYYRFRDVLEELEKEAKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q7</accession>
    <name>F4B3Q7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="9" type="ordered locus">Ahos_1211</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776010.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q7"/>
    <dbReference type="STRING" id="933801.Ahos_1211"/>
    <dbReference type="GeneID" id="10600687"/>
    <dbReference type="KEGG" id="aho:Ahos_1211"/>
    <dbReference type="eggNOG" id="arCOG04134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="OrthoDB" id="43788at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Enolpyruvate transferase" evidence="8">
        <location>
            <begin position="47"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="395" mass="43145" checksum="7DB8F1E73301A7B8" modified="2011-06-28" version="1">MKAKINSSYIEGKIRAPPSKSLGIRLIFLSLLTKVNLYASQELSDDLLVAKNAVNTLKNGGNYVYLGGSATTLRMLIPIALALGKKIKIDGDETLRRRPLNAIIKALKSAKFSSNSLPLIIEGKLEEETVIEGWESSQYISGLIYAYHIIGGGKIRIIPPISSKSYIEMTIDLFNRIGSDVKFEGNEILVNPRPLRGYEGEIPGDYALASFYALASLLTGGKIEIFGLYDPPDYFGDHNIVEIFSNMGAKSYYSSSWIVESTDEYSPIKINVNDVPDLAISIATLSSVANGCSEIQGIERLRIKESDRISTIISTLGAFGVSAKYTNDNITIKGIKKEKILKGSIICPSDHRIAMMAGVLSLIHGGEVDNAECVKKSNPLFWQDLIKLGGKISLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R3</accession>
    <name>F4B3R3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chorismate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1217</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776016.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R3"/>
    <dbReference type="STRING" id="933801.Ahos_1217"/>
    <dbReference type="GeneID" id="10600693"/>
    <dbReference type="KEGG" id="aho:Ahos_1217"/>
    <dbReference type="eggNOG" id="arCOG00245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_812860_0_0_2"/>
    <dbReference type="OrthoDB" id="34329at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010950">
        <property type="entry name" value="Chorismate_mutase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046825">
        <property type="entry name" value="PDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01791">
        <property type="entry name" value="CM_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20463">
        <property type="entry name" value="PDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="3">
        <location>
            <begin position="92"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="350" mass="40417" checksum="97672EC2DC4E379D" modified="2011-06-28" version="1">MSELEDLRKEIEEIDENIIKLLSKRFQISQQIGKIKKEKGIPITDENRESKVKEKWITLARKYGIPESFVESFLTMIFSYSKLYQINPKSKRRIVIIGYGGMARSLSSLFKISGQEVVITGRDLKKAEALSSEFNFVTMSVDSALKWGEIIISTLPPSAYFSDFMDETYKKMKAKIFMDITSSKAKIFKELEDKSIKYNFMYLSIHPLFGPYLYPVGEKVVLIPSKTSASIVRDIYEFFMDNGLVPLISSLEEHEKAMSVVQVLPHFYLLALSSSIQELSKELGIDFKKFETTNFREVYKIINRINEIKNVILEIQEHNPYADKAREFGVRELNNLYTQLMGKKNDALHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R4</accession>
    <name>F4B3R4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="5">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1218</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776017.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R4"/>
    <dbReference type="STRING" id="933801.Ahos_1218"/>
    <dbReference type="GeneID" id="10600694"/>
    <dbReference type="KEGG" id="aho:Ahos_1218"/>
    <dbReference type="eggNOG" id="arCOG01051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_1_2"/>
    <dbReference type="OrthoDB" id="6779at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000129">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051157">
        <property type="entry name" value="PDH/Transketolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825:SF1">
        <property type="entry name" value="TRANSKETOLASE-LIKE PYRIMIDINE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="8">
        <location>
            <begin position="7"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="312" mass="34652" checksum="4C0A5CD0BAFF63C5" modified="2011-06-28" version="1">MLQGSFYSIREAFGRTLVKLGEEDKDIVVITADVGDSTRASYFREKFPDRYFNVGISEQDMVNFAAGLAAVGKKPVVTDFAMFTMRAWEQIRNTTGRMNLNVKFTVTHSGYSDSGDGSSHQALEDIALMRTIPNFKVVVPADAAEVERSLPVIIKEQGPIYYRMGREYSPSITTGMDYKFEIGKAYVLKDGDDLAIMGAGVVLWDALKAAEELEKMGISTAVINVPSIKPIDENTIEYYARKTGRIITVEEHTIYGGVGSAIAEVVVQKYPVPMRFIGSTTFGRSARSQRELLDYYGINAENIIKKALELMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S3</accession>
    <name>F4B3S3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="6">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpo11</name>
        <name evidence="6" type="synonym">rpoL</name>
        <name evidence="8" type="ordered locus">Ahos_1227</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776026.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S3"/>
    <dbReference type="STRING" id="933801.Ahos_1227"/>
    <dbReference type="GeneID" id="10600703"/>
    <dbReference type="KEGG" id="aho:Ahos_1227"/>
    <dbReference type="eggNOG" id="arCOG04111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_5_1_2"/>
    <dbReference type="OrthoDB" id="24205at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06927">
        <property type="entry name" value="RNAP_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002233">
        <property type="entry name" value="PRK01146.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="7">
        <location>
            <begin position="13"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="91" mass="10281" checksum="2FDD780A6F87A5CC" modified="2011-06-28" version="1">MQIKILKSSDTYAEIEIEGEEHTLGNLLAGSLRKVEGVIYASYYQPHPLVNSIVLKVMTNGEIKPMDAIKKAIEIAENYANKYLEEIKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U9</accession>
    <name>F4B3U9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop10</name>
        <name evidence="8" type="ordered locus">Ahos_1253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776052.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U9"/>
    <dbReference type="STRING" id="933801.Ahos_1253"/>
    <dbReference type="GeneID" id="10600732"/>
    <dbReference type="KEGG" id="aho:Ahos_1253"/>
    <dbReference type="eggNOG" id="arCOG00906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196480_1_0_2"/>
    <dbReference type="OrthoDB" id="7259at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009623">
        <property type="entry name" value="PRK13130.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="56" mass="6571" checksum="C54981E266D3DA60" modified="2011-06-28" version="1">MTWLIRKCPVDGTYTFQEKCPKCNTPTIVPHPPRFSPEDKYVKYRIEAKKGIKLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W3</accession>
    <name>F4B3W3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (cytosine(72)-C(5))-methyltransferase</fullName>
            <shortName evidence="5">tRNA:m(5)C72 MTase</shortName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1267</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in several tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>cytidine(72) in tRNA + S-adenosyl-L-methionine = 5-methylcytidine(72) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61988"/>
            <dbReference type="Rhea" id="RHEA-COMP:15996"/>
            <dbReference type="Rhea" id="RHEA-COMP:15997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776066.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W3"/>
    <dbReference type="STRING" id="933801.Ahos_1267"/>
    <dbReference type="GeneID" id="10600748"/>
    <dbReference type="KEGG" id="aho:Ahos_1267"/>
    <dbReference type="eggNOG" id="arCOG00973">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07953">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02237">
        <property type="entry name" value="NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043699">
        <property type="entry name" value="NSUN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="93"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="348" mass="39512" checksum="1DF355B9876E31DD" modified="2011-06-28" version="1">MELDYDEFVIEELRKVYGKSLNSFLDSIKRPNSRLYVRVNTLKTTVDEVLQSMKGFKKDEDFPEAIYAEVKGPNKLESLDSKVIVDKKTAESVMLGADVYPPGIKKIIANEGNSVEVVSENGITVANGELVRLPGGKFMVKVYSSLYSSPKLADTKEIKEGKIYVQGKASMYVARIIDPQPGEFIVDMTAAPGGKLSHIYQLEPRARIIGFDHTYKKVEKMKQLFKKMNVNVQVFVHDSRYLSELGIKDVDKVLIDPPCSALGLRPKVYDKKTKTDLMNLHAYQKQFLNSAYEILKKGGEVIYSTCTVTEIENEEVVNDPRFDIEYMIRFHPQIHDMTGFFIAKLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X5</accession>
    <name>F4B3X5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774856.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X5"/>
    <dbReference type="STRING" id="933801.Ahos_0044"/>
    <dbReference type="GeneID" id="10599484"/>
    <dbReference type="KEGG" id="aho:Ahos_0044"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_0_2"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="358" mass="39807" checksum="BF4390264D217192" modified="2011-06-28" version="1">MKRKVVIVGGGNAGSIVANKLAKNTDLEVTVIEPSEYHYYQPGTVDIVGGIGKEEEMIKNTSDILHARWIKDYATKVDVENHTVFLKNGDKIDYDYVVIAAGVKNKKLEGFPEWHTIEGAKLMKTMADNFEGKKIVVGYFGLIKCPAAPFELSFILKQRFPKADITLVNPVAQPPQIQKPMAEILGKIAKELGVQVIRGFKIKEIDRQNKIIESENGEKINYDLAFIDTPIRAGEEFSNLVDNSGLIPVNKETLRFKDYDNVFAIGDITNITTPPKTGAIAHFEANYVVKEIQNDLYGQGKGKFNGSAACAVYNGYGKGAFIYMNYEKSYALGPSSIFFTAKKTFAHIYWLTVEGKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y3</accession>
    <name>F4B3Y3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Formate-dependent phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="7">6.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5'-phosphoribosylglycinamide transformylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Formate-dependent GAR transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">GAR transformylase 2</fullName>
            <shortName evidence="7">GART 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Non-folate glycinamide ribonucleotide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoribosylglycinamide formyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purT</name>
        <name evidence="10" type="ordered locus">Ahos_0052</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + formate + ATP = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.21" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774864.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y3"/>
    <dbReference type="STRING" id="933801.Ahos_0052"/>
    <dbReference type="GeneID" id="10599492"/>
    <dbReference type="KEGG" id="aho:Ahos_0052"/>
    <dbReference type="eggNOG" id="arCOG01598">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_1_3_2"/>
    <dbReference type="OrthoDB" id="9299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043815">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase 2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Formate-dependent phosphoribosylglycinamide formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
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        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK09288.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="purT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
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    </dbReference>
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        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PreATP-grasp domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
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    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="118"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
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    </feature>
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        <location>
            <begin position="366"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE92947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="395" mass="43964" checksum="B4937B70A9E95D80" modified="2011-06-28" version="1">MEIGTPLLEGSKKLMLLGSGELGKEIAIEAQRMGIEVVAVDRYDMAPAMHVAHRKYVIDMMDPSALKSIVKRENPDGIITEIEAINTDALIDLEDSGYKIIPNAQAVKICMNRIELRRLAAEKLKVPTTTYAFAESAEEVRKACRDIGFPCLIKPEMSSSGHGHVLINKEDEIEDAYKESISHARGKSKRVIVEEYVKIDTELTILTYRYNTGNGIVTKTFPPVEHQRPSYYYVESWQPATVQDDVVEKAKSYAIKVVEELGGVGIFGVEIIKSGNRVLFSEVSPRPHDTGMVTMASQDISEFQVHVRSALGLPTPEVRLLTPAASHVILAEREEWNPKYLNLEKALEIPGVQVRLFGKPYSYEKRRMGVVLANGSTVEEAKEKARKAAALILVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z7</accession>
    <name>F4B3Z7_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_0066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="GeneID" id="10599507"/>
    <dbReference type="KEGG" id="aho:Ahos_0066"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017896_3_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="171"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="349" mass="40419" checksum="AE9E1FF87D4A9C39" modified="2011-06-28" version="1">MKVNVVTHNLSNKSGEGFVVFSSIEMLKERNVDFVVSTFSKPRENYDLPVRYYLPFNFSRFDRYQRLLVWLSARKIRSDIYLNFTGIPIPLSSRGIHIIYGGASPFEVTKYSRSLFWKLYRFPFKIALRFLRNETNKAKFVANSFYSANTWKQLYGIEAKVIYPPVDVEDFFRAFHESGGKYILTIARIERGKFLERTIQLSHEVGIPAVIVGYLSDTKYYKELISLKSKLNANISFVLNASKEELIQVMKDACCYFHPTQGEHFGIPVVESMAAGLVPVVPKESGAAEIIPEFTYSTLEEAESKVKEAYIKHSSKSYEMKEIASKFSKARFKEEFWQYIINIYNNNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B428</accession>
    <name>F4B428_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved archaeal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774909.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B428"/>
    <dbReference type="STRING" id="933801.Ahos_0099"/>
    <dbReference type="GeneID" id="10599539"/>
    <dbReference type="KEGG" id="aho:Ahos_0099"/>
    <dbReference type="eggNOG" id="arCOG10298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943930_0_0_2"/>
    <dbReference type="OrthoDB" id="57125at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14706" checksum="8C58EF71E17AAF79" modified="2011-06-28" version="1">MPDGKIPLQAEVYLDGEKVEAKVFLHVKGYKRARVTHIDVEGDKIRGLLRPRHSVYPLVEWKGNEVIFPINEHKIRIIIPEITLNFNGNLYVGGKGKGIFLGFHRNEIRQLEEIAKQKGVEPIGRESKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B466</accession>
    <name>F4B466_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7 8">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfdA</name>
        <name evidence="10" type="ordered locus">Ahos_1291</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 7">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the prefoldin alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776090.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B466"/>
    <dbReference type="STRING" id="933801.Ahos_1291"/>
    <dbReference type="GeneID" id="10600772"/>
    <dbReference type="KEGG" id="aho:Ahos_1291"/>
    <dbReference type="eggNOG" id="arCOG01341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792160_0_0_2"/>
    <dbReference type="OrthoDB" id="19084at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="147" mass="16374" checksum="29A020044FFE0BBA" modified="2011-06-28" version="1">MSDQQENKVVISLDDLLAQAQLLKKQIDDLSAARAELLDSVASIEAAKASIQELKSQQEMLVSSDKKGYLMFKINNQPPQKVLVYLGLSYYVEVDMDTALKILDDRENELKEASTGLDKKLAESKAAYDQIVDILNQIQAQAQQKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C4</accession>
    <name>F4B4C4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">anthranilate synthase</fullName>
            <ecNumber evidence="2">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1349</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776147.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C4"/>
    <dbReference type="STRING" id="933801.Ahos_1349"/>
    <dbReference type="MEROPS" id="C26.955"/>
    <dbReference type="GeneID" id="10600835"/>
    <dbReference type="KEGG" id="aho:Ahos_1349"/>
    <dbReference type="eggNOG" id="arCOG00086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="OrthoDB" id="3321at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000003">
        <property type="entry name" value="Anthranilate synthase component II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="193" mass="21620" checksum="29EA7BFEB2523B91" modified="2011-06-28" version="1">MDLTLIIDNYDSFVYNIAQAVGELGSYPIVIRNDEITVKGIERINPDRIIISPGPGSPDKKEDIGIVIDVIKQLGKRIPILGICLGHQAIGYAFNAKIRKAKVVFHGKLSTIIQNSSAVIYEGLPKEFKATRYHSLVIDDVKPPLIIDAYSKEDNEIMGVHHEEYKIFGVQFHPESIGTQVGQKIFYNFLNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D3</accession>
    <name>F4B4D3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774939.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D3"/>
    <dbReference type="STRING" id="933801.Ahos_0129"/>
    <dbReference type="GeneID" id="10599569"/>
    <dbReference type="KEGG" id="aho:Ahos_0129"/>
    <dbReference type="eggNOG" id="arCOG01367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192571_0_0_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43242:SF1">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="1">
        <location>
            <begin position="51"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="26025" checksum="87F11DF7D45FE974" modified="2011-06-28" version="1">MIGITDEGELARALTNAIDDEFIFIDSPKKVYETKPEIVIHTLEDNSNNPSMWNLNTWFAINIAKAANKIGSLNVYFSTFMIYDGKKGYYSETSTPSPLNYYGMTKLVGETAIASLGNYLILRLGAVYSPSYRGILFHIIKALIKKGIAKCNTNLYLSPISVKDVAYAVKYLLKKDARGVINLAGKRFSEYDICKKFSDDYGGIIIPIEGKYFDFSLDNWLLRSFGLKIDGI</sequence>
</entry>
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    <accession>F4B4E0</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="10599576"/>
    <dbReference type="KEGG" id="aho:Ahos_0136"/>
    <dbReference type="eggNOG" id="arCOG05321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155017_0_0_2"/>
    <dbReference type="OrthoDB" id="24524at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="121" mass="13754" checksum="88FC4E34B234B21E" modified="2011-06-28" version="1">MSINPVQIAQLPYTNRIDNIQKIIQTLLSMDPKTAESAVKDLLTALSKASDEDYKNWCEGTMRVISMFDDSVIKAILSLRMKVVSEMPKEVQDRDSKMVMEVLNSLDESVKEKLMKNMPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E6</accession>
    <name>F4B4E6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 domain-containing protein</fullName>
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    </protein>
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    <organism evidence="1 2">
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        <lineage>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0142"/>
    <dbReference type="GeneID" id="10599582"/>
    <dbReference type="KEGG" id="aho:Ahos_0142"/>
    <dbReference type="eggNOG" id="arCOG03708">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_1_0_2"/>
    <dbReference type="OrthoDB" id="28552at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="157" mass="18221" checksum="2866DA1856EDB18A" modified="2011-06-28" version="1">MVQELPKPWFNLEAYKKTRLEEAKYEAEIARKFLEQNLIRNAAGKAFQAWKALVAALAVDKRNELEKLYPGYVKIRGNKRVKRVDWVILIMPTTLLKEVAMIISDEVNNFTNMALLLHQYQYNGPDKEGVVSFYRSDEMAKQDILRLLSEIDKLTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I2</accession>
    <name>F4B4I2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hydrogenase 4 membrane component (E)-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_0179"/>
    <dbReference type="eggNOG" id="arCOG03626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088957_3_0_2"/>
    <dbReference type="OrthoDB" id="57556at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038730">
        <property type="entry name" value="HyfE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38601">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38601:SF1">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="193"/>
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    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="206" mass="23120" checksum="3B94EE2478DFDD9B" modified="2011-06-28" version="1">MIKEEIIELISVIIIITAFYIQGQAYYKPLVKMSGVQSIILAILSTFLGIITGIVDYFILAIIIVTLRGIITPYVLIKMLGNKFGEREKIKGVASLLVIDLAFFFIAIIIIYEFIIGRIFHQVELLFPLSLFFQGLYLIVSRNSTPAQIIGYIEEENGIVMLGLFLIPLPLLVEASVFLDVLGLVVISSLLIFEKREHKALEELKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I7</accession>
    <name>F4B4I7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
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    </protein>
    <gene>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774993.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I7"/>
    <dbReference type="STRING" id="933801.Ahos_0184"/>
    <dbReference type="GeneID" id="10599624"/>
    <dbReference type="KEGG" id="aho:Ahos_0184"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="298"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="382" mass="45160" checksum="DB3CFFF45C53FA42" modified="2011-06-28" version="1">MARRDNKAIRATVSMKIAVSDSLLALVNNYVKALRFTLFWLKENVKNPEEKGVLSKVHEALYEKLREEYNLPSKVAEDCYRDAISIYNGWYNNPRRGRFPRVYKPTVWLTPKASYNVDFDKMTVRIASVGELSILGYPRNLREYMSWRMREARLTVKGDKAFLKVVFEKEEESVEPKESVAVDINMAEVVVGKDDKHYVRIPTRLDEVHHWKSLAENLQRKYPRRWRENKKILHRIHSFHLKARRIAEDFARKVGRWVIEIARDFSANVIKLESLKNLIKNVDKLPKEFHDKLYLMQYRRLQYWISWQARKHGMTVEFVNPKYSSVSCPKCGQKMREVSHRWFKCSCGYENDRDVIAIMNLNGRGSLTLSTAPQMRDVRANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M5</accession>
    <name>F4B4M5_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1372</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776170.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M5"/>
    <dbReference type="STRING" id="933801.Ahos_1372"/>
    <dbReference type="GeneID" id="10600859"/>
    <dbReference type="KEGG" id="aho:Ahos_1372"/>
    <dbReference type="eggNOG" id="arCOG04160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084972_0_0_2"/>
    <dbReference type="OrthoDB" id="18600at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="269" mass="31136" checksum="A7E93A5831175703" modified="2011-06-28" version="1">MPRKKKTDEENKKESSNEEGKTKRKKTQKKEKFVDAEALLDEYLDEVVLGLGISFLKLKPEEYKEILKEPFVAAVGQVKTKPKSKTIINRLTANRDAMMEYIAMRLVRIIDIEKMNCEQFEFVVYNTKKAIVDLAPKLYREALKRNRKDLIDVLRYNWNLYGIVSPIVCPKCGFSSIMPDLSCKVCEYVMSMKELKSQINVIQTLKDYYSLDPEGFKEILTTGYFYYTAEGPIPPSKFRPSPQVLAFEIVLNKDEKKTLSSIYSNPHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M9</accession>
    <name>F4B4M9_ACIHW</name>
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        <recommendedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776174.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M9"/>
    <dbReference type="GeneID" id="10600863"/>
    <dbReference type="KEGG" id="aho:Ahos_1376"/>
    <dbReference type="eggNOG" id="arCOG03879">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172290_0_0_2"/>
    <dbReference type="OrthoDB" id="40936at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11246" checksum="E9D610546C58D517" modified="2011-06-28" version="1">MSSRRRKKKESDETEDVENKLKVVYSDKDVVVMTAPNEEELKQILLDLLSEKPMNLKELHSKLSGIASEDKIRRALASLTEKGQVTLLEDGRYAKLGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q0</accession>
    <name>F4B4Q0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5622 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776195.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q0"/>
    <dbReference type="STRING" id="933801.Ahos_1397"/>
    <dbReference type="GeneID" id="10600886"/>
    <dbReference type="KEGG" id="aho:Ahos_1397"/>
    <dbReference type="eggNOG" id="arCOG04104">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188105_0_0_2"/>
    <dbReference type="OrthoDB" id="39186at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041043">
        <property type="entry name" value="DUF5622"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18533">
        <property type="entry name" value="DUF5622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5622" evidence="1">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="66" mass="7788" checksum="05BCA9135067F149" modified="2011-06-28" version="1">MLKHKKYLYVDTGKYFIKVRVLKNRDENSPDSYIVVSKNIKKPRNALVKKLEDLPIEVRDKISKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R4</accession>
    <name>F4B4R4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776209.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R4"/>
    <dbReference type="STRING" id="933801.Ahos_1411"/>
    <dbReference type="GeneID" id="10600901"/>
    <dbReference type="KEGG" id="aho:Ahos_1411"/>
    <dbReference type="eggNOG" id="arCOG04324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156326_0_0_2"/>
    <dbReference type="OrthoDB" id="45368at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="11771" checksum="03DBBDE6461D0CA6" modified="2011-06-28" version="1">MSQPAYKVEFEGKAKIGQIMGNLASIQLKPEDFSSPLSLQMALSRIYNELMKAMSQGPQTHYVADVKFTDSLGNPVSIGVDFGDKIPPLSKQDVKVKITIEFYDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V1</accession>
    <name>F4B4V1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775033.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V1"/>
    <dbReference type="GeneID" id="10599664"/>
    <dbReference type="KEGG" id="aho:Ahos_0224"/>
    <dbReference type="eggNOG" id="arCOG08520">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2597490_0_0_2"/>
    <dbReference type="OrthoDB" id="36739at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="79" mass="8815" checksum="441ADCA3D1ADB2BD" modified="2011-06-28" version="1">MEHKNPPTIIPSSQFPTSEQPVVMVVDVMLRCKLDNQVMPASMALEHQQMHIKKGEDPCFVIETAPGKQKGEESTRGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V3</accession>
    <name>F4B4V3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Urease accessory protein UreH-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775035.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V3"/>
    <dbReference type="STRING" id="933801.Ahos_0226"/>
    <dbReference type="GeneID" id="10599666"/>
    <dbReference type="KEGG" id="aho:Ahos_0226"/>
    <dbReference type="eggNOG" id="arCOG06008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942587_0_0_2"/>
    <dbReference type="OrthoDB" id="56710at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="34767" checksum="A305D51754C5DE3D" modified="2011-06-28" version="1">MIDWLVINLWNPQNVDVVSALVIAYLLGILHGVTPDEHTWPITFSYSVGTFSTKGGAKTGLIFSSGFTLQRSILSELAYLALAGVFMTTTAFGVTYIFVGIAMAAAGVYIAKKGGYPHWHYLEKRLGEISGIHKKGSELQEQELQHKINPAYVNEKDLTKPVPVKLAFIHGFIAGFGFGAFALIVYTVLAPTMPNAYLGWVPGMLFGLGTMTAQVFFGTAFAAWLTRMKNLTFQGIAVVGKTITKTVLEYGGIAFIVGGIAILLYPPLLTYNIVTPIKVHNLHSLGIGFFLVILSVVIFGIYGYKQGVKTAIKLGYTNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z4</accession>
    <name>F4B4Z4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PaREP11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0268</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775075.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Z4"/>
    <dbReference type="STRING" id="933801.Ahos_0268"/>
    <dbReference type="GeneID" id="10599708"/>
    <dbReference type="KEGG" id="aho:Ahos_0268"/>
    <dbReference type="eggNOG" id="arCOG02108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153000_0_0_2"/>
    <dbReference type="OrthoDB" id="42093at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.580">
        <property type="entry name" value="bsu32300-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037038">
        <property type="entry name" value="HepT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052379">
        <property type="entry name" value="Type_VII_TA_RNase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047751">
        <property type="entry name" value="HepT_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33397:SF5">
        <property type="entry name" value="RNASE YUTE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33397">
        <property type="entry name" value="UPF0331 PROTEIN YUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="HepT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="136" mass="15994" checksum="175847B398C91876" modified="2011-06-28" version="1">MAILDKLLENLKDCTSKLDEANNVDLDSWFNLFAILHLLQVQAQSFIELVQTLLSNMGISCQGYRDSIRKLYEKNLITAEERDFLISVVGFRNIIVHEYATVNPIFVKKIMENKEYRRLLEIAYNLREKAKNYWDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B516</accession>
    <name>F4B516_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B516"/>
    <dbReference type="KEGG" id="aho:Ahos_1435"/>
    <dbReference type="HOGENOM" id="CLU_2230234_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="105" mass="12123" checksum="365D24DF150C7710" modified="2011-06-28" version="1">MSPFILVKMQVTASLIMSIDVFTLSIFKSFSLIGNFSMISNYADCHLNLDYMIYLIKGENGNFLRGWISNKISLIARSAMREIIMRRDSHLSFSRSRIKYQSKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B543</accession>
    <name>F4B543_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1462</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776260.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B543"/>
    <dbReference type="STRING" id="933801.Ahos_1462"/>
    <dbReference type="GeneID" id="10600958"/>
    <dbReference type="KEGG" id="aho:Ahos_1462"/>
    <dbReference type="eggNOG" id="arCOG00541">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF52">
        <property type="entry name" value="MRNA 3-END PROCESSING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="3">
        <location>
            <begin position="231"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="421" mass="46869" checksum="3A1E573AE60BC77D" modified="2011-06-28" version="1">MSYHLKILGGGQEVGRAAIEVTGPDGSIVLDYGVNFNPDDTPNFPLQEMPSKVKAFIVSHAHLDHVGALPIYQISGSKPIYGTSITKEIGELILKDFLKISGPRVPYEWVEVKKTLDNFNTIKYNEEFEVGSFRIKTSSAGHIPGSAITVVKTNKGDVTYTGDINITNTKLMKPADLDIMRDSRVIVTEATYGKFNHPQRKSVEDEFYNAIMEVLEEGGTVLVPAFSLSRSQEILSLLAERNIPYPVYYDGMVKTIMEIMINNPEYINNYEALKKAYNEFHYVNGLQDRKKAYKSNGVIVASAGMLKGGPAVYYFKKIADNPKNAVFLVSYQADNTPGRKLLELGKFDEESPLLKARLQLFDFSSHAGRDQLMQILKASKSLEKVVVVHSSADSAQYFADHVKQELGVEVIVPENGQEIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B549</accession>
    <name>F4B549_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate-starvation-inducible E-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1468</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776266.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B549"/>
    <dbReference type="STRING" id="933801.Ahos_1468"/>
    <dbReference type="GeneID" id="10600967"/>
    <dbReference type="KEGG" id="aho:Ahos_1468"/>
    <dbReference type="eggNOG" id="arCOG06050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143948_0_0_2"/>
    <dbReference type="OrthoDB" id="42796at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020948">
        <property type="entry name" value="P_starv_induced_PsiE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06146">
        <property type="entry name" value="PsiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="16010" checksum="A27908A692BAD2AA" modified="2011-06-28" version="1">MSSENLSKLVIKITSITVQILLIIGLIIVLLYTVTQTIESFQISLIDVASIILENSLLIIVFLEVYLSVVDFFHGKGRSVVYVMDATLSFVLREIIIGILTGSVTDIDLLAMSGAIGIIASGRFLLTGKNLRSIRRRKVNKERSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B551</accession>
    <name>F4B551_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome partitioning ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776268.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B551"/>
    <dbReference type="STRING" id="933801.Ahos_1470"/>
    <dbReference type="GeneID" id="10600969"/>
    <dbReference type="KEGG" id="aho:Ahos_1470"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090891_1_0_2"/>
    <dbReference type="OrthoDB" id="34582at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="221" mass="25439" checksum="1A69176669D4782B" modified="2011-06-28" version="1">MIRITFASPKGGVGKSTIIYYVTKLLSDKFKTLIVDLTDSATLSRFFGIQNNILADDNYFADKGNIGVISFSRISNNDNLDLEKITLKYEEILVDYSLILVEYPIHFYSKSIKIEYEIFNSLSKTKNYIFSVTIPQDIIIKSTLNYASSLISFLSSINKDIYDEALIINMIKDTKIKNIANYHNNVFSIKFNYDLIFKGFYNVDPLDDFLQISKFIENLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B560</accession>
    <name>F4B560_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776277.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B560"/>
    <dbReference type="STRING" id="933801.Ahos_1479"/>
    <dbReference type="GeneID" id="10600979"/>
    <dbReference type="KEGG" id="aho:Ahos_1479"/>
    <dbReference type="eggNOG" id="arCOG00663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_2_2_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="229" mass="25939" checksum="694688D34AC03B6A" modified="2011-06-28" version="1">MKAVILAGGYGKRLRPLTDDKPKPLIEIAGKPILEWQILWLKSFGITSFIILAGYKKEVLVEWVTKNKARLGIECLFSMEEEPLGTGGAIHQVKSFLNEDFIAVNGDILTNLDIRELKQVDETAIASISLVPLRSPYGVVQTEGDKIIKFVEKPVLKDYWINAGVYFLKSSIFEYLPEKGDIERITFPTLAEKGLLRGKKFENVYWRSIDSVKDVEEASNEIPQVFNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A6</accession>
    <name>F4B5A6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0309</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775116.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5A6"/>
    <dbReference type="STRING" id="933801.Ahos_0309"/>
    <dbReference type="GeneID" id="10599750"/>
    <dbReference type="KEGG" id="aho:Ahos_0309"/>
    <dbReference type="eggNOG" id="arCOG04310">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_2"/>
    <dbReference type="OrthoDB" id="275197at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00567">
        <property type="entry name" value="ACAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF2">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="8">
        <location>
            <begin position="123"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="231"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="386" mass="42884" checksum="3C5DFBD8ECC9C193" modified="2011-06-28" version="1">MDFEFSDEEKIFQENLREYFSKSLRPRLREIDEKGIPKDFILDASKIGLWSLTFSEDVGGQHASFTLASIAAEEIARADFTMATAVFFLLENSWGYVLDKYGSEELRKEVLPKVASGENFLGIASTEPSGGSDVANLKTSAKKEEGKYVINGEKAYISGVMEAKEMGGGHLTLVRTGGKGHKGISMIYVPINSEGITVSRIENMGRMGISTGIIKYDNVRIDEKFLVGEENKGFYYAMDGFNHARVLVASACIGSAEAILEEGLKYIKEREAFGVKLKDLQSIAFEAAELYTKLEMARLLVYKSAWMLDHEEKFKDEIPKFSAMAKLIAPQTAFDVIKSVMIWFGAYGYTKDALIESGMRGLMSYLVGAEGALNIMKLIISREILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C7</accession>
    <name>F4B5C7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775137.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C7"/>
    <dbReference type="STRING" id="933801.Ahos_0330"/>
    <dbReference type="GeneID" id="10599771"/>
    <dbReference type="KEGG" id="aho:Ahos_0330"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_2"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="271"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="9">
        <location>
            <begin position="442"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="410"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="585" mass="63460" checksum="BAB3E98E5408A15A" modified="2011-06-28" version="1">MGGIFGFICNSPKDVNVAINGLKRLVYRGYDSAGIVYMNGKLTVKKIVGNITKVDLQISGTSRIVLAHTRYASRGWPTLENAHPLFDCNKKIALVMDGVIDDYEEIRDKLIKQGHKFVSTTDTEVLPHLLENSQDYIRSSIEIIKNIKGIYSFAFIVDGINKIFAGSAGQPIVIGITQECKYVSSDLPSLNGFAENAIILPENSVAEISEDYVKIFDIQGNQITVQPKRVKYQEEIVDKGGYPHFMLKEIYDIPSALISSFTSLMEKYLSLSAMIVYGAKNVYVIGNGTSLHAGLISSYYFAEVGINVNVVSAAEFPYYALENIGTGSVIIAISQSGETADVIRSVKMAKQRGAVIVGITNSVGSRLALESNVYLPITAGPELAVPATKTFTSTLVVLRTLSLYTGLNSGKKSKSDIEKLKEDLNNLSKALSSNLPQIEKQAEDAVAKINSESMYVTSSGINFPVAMEGALKFKEASLTHAEGIQLGELLHGPIALTNKGYPIVVIKPAEEQAYDLYNKVVKSISDRGSPIILISNDGMLKSVETTRELSPIANVVPLQLMAYKLGVKKSLPIDTPPGLVKAVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H6</accession>
    <name>F4B5H6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger CDGSH-type domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1517</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776315.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H6"/>
    <dbReference type="STRING" id="933801.Ahos_1517"/>
    <dbReference type="GeneID" id="10601017"/>
    <dbReference type="KEGG" id="aho:Ahos_1517"/>
    <dbReference type="eggNOG" id="arCOG01667">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145019_3_0_2"/>
    <dbReference type="OrthoDB" id="5781at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CDGSH iron-sulfur domain, mitoNEET-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FeS-contain_CDGSH-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042216">
        <property type="entry name" value="MitoNEET_CISD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09360">
        <property type="entry name" value="zf-CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00704">
        <property type="entry name" value="ZnF_CDGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron-binding zinc finger CDGSH type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="68" mass="7945" checksum="6AD5AEA83AA5D848" modified="2011-06-28" version="1">MARLVKHDRNRPYMIKADNGQVFYVCACGLSRNKPFCDGSHKRTLDEEEGSVYVYDENGRIKLQNFYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K0</accession>
    <name>F4B5K0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1541</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776339.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K0"/>
    <dbReference type="STRING" id="933801.Ahos_1541"/>
    <dbReference type="GeneID" id="10601041"/>
    <dbReference type="KEGG" id="aho:Ahos_1541"/>
    <dbReference type="eggNOG" id="arCOG01067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815399_0_0_2"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051169">
        <property type="entry name" value="NADH-Q_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42913:SF3">
        <property type="entry name" value="64 KDA MITOCHONDRIAL NADH DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42913">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="320" mass="35430" checksum="219CB945B4935043" modified="2011-06-28" version="1">MRIVILGGGFAGLSALKTHDSIMIDNKDYFILTHKLIDVVKTGDPSIAKIPYPKVLKATVKSIDFRKKVVITTSGEIAYDKLIISLGYSQKLIEGVTKFENIEDALRIREGLLRAKTVVIIGGGNLGVELASLAREMGKEVYLIEGQSRLLNFMSQESSAYAEKKLREMGVNVLLNTKVEKIEDNTVYAEDQKIRGDLIVSSIGFKGPSILKELGLTTINDRMVVNEYLQSVDQEDVYGAGDCSTTKEFIPMSAQVAVQAGRTAMLNAIGFERKFSYRQYAIIVRIGNEYFGDLLGKFVRGRVAELAEKLGIHRAIKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5L3</accession>
    <name>F4B5L3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ParB domain protein, nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776352.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L3"/>
    <dbReference type="GeneID" id="10601054"/>
    <dbReference type="KEGG" id="aho:Ahos_1554"/>
    <dbReference type="eggNOG" id="arCOG07935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049560_0_0_2"/>
    <dbReference type="OrthoDB" id="44079at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16404">
        <property type="entry name" value="pNOB8_ParB_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="440" mass="50876" checksum="F625FFD50C58BC63" modified="2011-06-28" version="1">MSKLKEYKEISIEEIKEVDEFRTFIPENNMYDKIKEDIAKNGIKVPLIVNQNYELINGYTRLKIAQELGIKKVPIAVYETTDRADEYDLLVSTNLTQRQLTRVQALALIEKALEEKTKILKKNQNSENNPKETKVERVPVQLSESQNKKIQSLTDLRNGVKEELEKYNVKVDDRTLDYYIRIKENAPWLTDYILKGNLGIYPAYSIYTKLQEMGLLEAVAKLSPYERNTLLTDGRKIISGERKDLLEEIVNHKITVSQAINKLKTEGKLQRSKKSRVKDKDLDESEDEGEELEADEREEAESEEYDFVGEWQKAEEEEVKQQLTPQLKTEELDEGQQISRLESKPDQGQLESQKEMDLITELKTKGYAELPFEVALVKIEGKCYAINVGALHDLEQGVEKWKELADFLSKHDIIIPGEAEGLYVIPWKLLGRCNEWQSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5L8</accession>
    <name>F4B5L8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3093 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L8"/>
    <dbReference type="KEGG" id="aho:Ahos_1559"/>
    <dbReference type="eggNOG" id="arCOG08543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1943735_0_0_2"/>
    <dbReference type="OrthoDB" id="43707at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021443">
        <property type="entry name" value="DUF3093"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="15046" checksum="2EA89AC709EC60C6" modified="2011-06-28" version="1">MLRIHTKKGDKLFLYVQIILWLIYVMIDLGLFTNKLLLFATIALALSVVLIALVLTSYSYLNVRITPERVEVGKWKIPLDQVREIRVLRSNGPTADLIIVYEGGERIIKEVKNWKEVLETFQRYKENIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P0</accession>
    <name>F4B5P0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated RAMP-motif protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P0"/>
    <dbReference type="STRING" id="933801.Ahos_0364"/>
    <dbReference type="KEGG" id="aho:Ahos_0364"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782421_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09726">
        <property type="entry name" value="RAMP_I_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="145" mass="16433" checksum="F17423969FA0E118" modified="2011-06-28" version="1">MFIKYDDKIYVIPGSSIKGLIRKNMKILNCDTSVLGSEFGEKSVTSKVIVSWGYITEEKKKKVRYGIRVNKELGIVEKGALFSYEIIPENLEIRFDIIPIIDLTNKERECLRKALLLMRYSTIGWGGSKGIGIVEEVKLDDALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R6</accession>
    <name>F4B5R6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775197.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R6"/>
    <dbReference type="STRING" id="933801.Ahos_0393"/>
    <dbReference type="GeneID" id="10599833"/>
    <dbReference type="KEGG" id="aho:Ahos_0393"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="298"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="382" mass="45158" checksum="9F3A0C9C7BEB05F7" modified="2011-06-28" version="1">MARRGNKAIRATVSMKIALSEPLLALVNNYVKALRFTLFWLKENVKNPEEKGVLSKVHEELYTRLREEYNLPSKVAQDCYREALATYKGWYNNPRRGRFPRVYKPTVWLTPKASYNVDFEAMTVRIASVGDLQILGYPRNLKEYMSWRMKEARLTVKGDKVFLKVVFEKGEENVEPKESIAVDINMNEVVIGKDDKHYVRIPTRLEEVHHWKSLAENLQKKYPRRWRENKRILYRVRSFHQKAKRIMEDFARKVGKWVVEIAEDFGANVIKLESLKNLIKNVDKLPKEFHDKLYLMQYRRLQYWIEWQAKKRGMTVEFVNPKYSSVSCPKCGQKMKEVSHRWFRCSCGYENDRDVIAVVNLNGRGSLTLSTAPQMRNVRANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5T0</accession>
    <name>F4B5T0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775211.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5T0"/>
    <dbReference type="GeneID" id="10599847"/>
    <dbReference type="KEGG" id="aho:Ahos_0407"/>
    <dbReference type="eggNOG" id="arCOG10617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399666_0_0_2"/>
    <dbReference type="OrthoDB" id="44150at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="194" mass="22063" checksum="207C3269340D86BB" modified="2011-06-28" version="1">MEKFFQIDNVDLNSIHDRITSEIQNQGMEIHRDEPRDNGFKLFVRRGEEHGEVEVFNDWGGVKVITHGRLEWDIMNIVEPIMQQPQAAPQGMPTQVQQNRPPMPSPSPAMPQQPMMPHPMVPQPNPAMQPAIVPHPTMPPTTQAAITPEHQQFLGILTQNGYQIMVNNANQGYFFIRGRKGNFVIDIEGREQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V1</accession>
    <name>F4B5V1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">prolyl oligopeptidase</fullName>
            <ecNumber evidence="2">3.4.21.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Pro-|-Xaa &gt;&gt; Ala-|-Xaa in oligopeptides.</text>
            <dbReference type="EC" id="3.4.21.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.21.26" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775232.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5V1"/>
    <dbReference type="STRING" id="933801.Ahos_0428"/>
    <dbReference type="MEROPS" id="S09.002"/>
    <dbReference type="GeneID" id="10599868"/>
    <dbReference type="KEGG" id="aho:Ahos_0428"/>
    <dbReference type="eggNOG" id="arCOG01647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011290_1_1_2"/>
    <dbReference type="OrthoDB" id="31240at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF2">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="3">
        <location>
            <begin position="381"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="581" mass="66277" checksum="D11DF9C2143EFFD2" modified="2011-06-28" version="1">MDEFEYLEDLNNERTKSFIENENKKTEEKLGKRAKELYPKLLEIYKEPYVLSMFAYDENNPVILLYGEKNQVLLGNKVIYTSPKDYVASSVWKVYNSKEIGVSIEKKGSDKITTFLISTDGKITELGEMVESPFYFKNELCYIKSYRYSPPPDGGEYPADRVFCGNEIVYGKDLKAGEFVSIRTFGDFITLIRSKGWRYSELYAGEGFDSLKKIDEGEVIDVIDYVQGSLIYQKNDSVYLDKKKIIKSDYPDLGVSSLKETLAVEVIKDYRTPLLFYSINGDKIGEEIHDNIQFMDSEGHSLFIVETSFNYKFKVSRRVKEKSEETIMQYGNYDVKVTDIYVKGEVLLHGFLLTKTNNSKGVIVYGYGGFRISLLPSYPMSTRLLLDEGYSVLITNLRGGYENGEEWHKAGMLLNKKNVFKDFSEFLRLVKSLGGKTIAMGGSNGGLLVGATENEYPNVIDCAVIGHPVLDMLRYDKLYVGKYWVEEYGDPNDPKYRDYLLSYSPYHNLKKGPPKTFVYTGINDDRVHPAHALKYVAKSEKLGNDVMLFVNDSGHAIADPESEAREYSYVLAFIEECTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Z5</accession>
    <name>F4B5Z5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">dTDP-glucose 4,6-dehydratase (RfbB-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Z5"/>
    <dbReference type="STRING" id="933801.Ahos_1615"/>
    <dbReference type="KEGG" id="aho:Ahos_1615"/>
    <dbReference type="eggNOG" id="arCOG01371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161798_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="98" mass="11901" checksum="A193FB470D4CBEDF" modified="2011-06-28" version="1">MERAEWKGEVYNIPGKQRVTNLDLLKLLGEVMGKEIKIKFVSDRPGHDRRYCMNTSLSYETTPLKDGLKKTYEWYLENEWWWRPLIDDKFFKEDAPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B613</accession>
    <name>F4B613_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776426.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B613"/>
    <dbReference type="STRING" id="933801.Ahos_1633"/>
    <dbReference type="GeneID" id="10601133"/>
    <dbReference type="KEGG" id="aho:Ahos_1633"/>
    <dbReference type="eggNOG" id="arCOG02960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764197_0_0_2"/>
    <dbReference type="OrthoDB" id="34215at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M28" evidence="1">
        <location>
            <begin position="158"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="362" mass="41306" checksum="9B94AD3D597C0D0A" modified="2011-06-28" version="1">MISLDLLKLGEAIHGSEKEKEILSILEKRLKNDNKRIEVKTKEWVINNFSVKLNGKEVTSSLAPYTKGYVKGKVGREILAFPFPDHPFKVKDFYSYALSQGAEGIIFYEEGKIRRIIMPDVKIPVIFLPFKPEGYVEIEAESFLRDSTSYNLEFTIKEGDDYILLGAHVDHWLSGFHDNLFSIDVVLNSLTEVKNHGLKVVFFSSEEGPKCCTGSLQHPKDDIFTAIILDALYPSRVVYSSTPDLWDFAGLFPLKRIEMPTPFSDHFSFVQKGIPSLVLYNDDLIPYYHSDKDLPNSRDEKFLTEISESVKLLLHELDKLSMKDLNERMSKFGERKFVVDYVNLTTSFAISSNSLGPRLKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B616</accession>
    <name>F4B616_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidase U32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776429.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B616"/>
    <dbReference type="STRING" id="933801.Ahos_1636"/>
    <dbReference type="GeneID" id="10601136"/>
    <dbReference type="KEGG" id="aho:Ahos_1636"/>
    <dbReference type="eggNOG" id="arCOG03202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053295_0_0_2"/>
    <dbReference type="OrthoDB" id="120329at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051454">
        <property type="entry name" value="RNA/ubiquinone_mod_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217:SF10">
        <property type="entry name" value="23S RRNA 5-HYDROXYCYTIDINE C2501 SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217">
        <property type="entry name" value="PEPTIDASE U32 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="383" mass="44345" checksum="EC246DCFD4BE2158" modified="2011-06-28" version="1">MRLVVGTNFDDKLIDELKKYPVRYIFGSKTKTLTGHGRASFVLPQVDDERLKEHIQIAHDAGIKFLYTMNTATLNGKEYSQKFLESLKKEIDNLVNLGVDGFIVGMPFLVHFIKNEYPDLEISISSFSRVYNIREFEEYVNMGADTIILHEDDNRNFSLLNSLSKFRNKVDIELILNNSCLWGCPYRRTHDIISSVTSQSDSEVNVWFEYPILFCATDVRNDLANIIRMRWIRPEDLRHYEELGIDRFKIASRNKKTDWIVRAVKAYSQGRYEGNLLDIVSYPQGRAVPAVMKKINGPNDYDVLTQVYVDNTKFPPNWLSYFKYNSCETRSCEECRYCDIIAEKVITVQGKPMSEISLPKIKPPIELIPRFSKDASNEGSKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B624</accession>
    <name>F4B624_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VapB-type antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776437.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B624"/>
    <dbReference type="STRING" id="933801.Ahos_1644"/>
    <dbReference type="GeneID" id="10601144"/>
    <dbReference type="KEGG" id="aho:Ahos_1644"/>
    <dbReference type="eggNOG" id="arCOG07235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175270_3_1_2"/>
    <dbReference type="OrthoDB" id="39930at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="10035" checksum="F5DE227A2ECBB94B" modified="2011-06-28" version="1">MKVISVKVPEEIYEKMKMHKEINWSEVIRNAIISELNELEGITTGNELIERLKRLGVDEKDINVEPPQGEDEFQKELKKKSTIRTP</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="aho:Ahos_0442"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="99" mass="10819" checksum="1702849CBF009F72" modified="2011-06-28" version="1">MNLYPILSPWASPESLPLPLRPFSGITPPIRVLPSDGETRTSSSYRHTFRENPSTSGVTVLIFTIKVYKLHGLSETADNGVHQSSGTGGFITKDKISVY</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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    <dbReference type="GeneID" id="10599942"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="36764at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="102" mass="12255" checksum="F556B3F1615775BC" modified="2011-06-28" version="1">MSFSKKFYDMQDFILSRTALEKVKRHVEERKENSIYKWISSELNYFISKYENESDLKECIKRIKDGISAENYSYILQGSKECIETLSKKINELYESLMEQDQ</sequence>
</entry>
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    <accession>F4B6F2</accession>
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        <submittedName>
            <fullName evidence="2">Cysteine-rich domain protein</fullName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="933801.Ahos_1695"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43682">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43682:SF1">
        <property type="entry name" value="LACTATE UTILIZATION PROTEIN C"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02754">
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        <property type="match status" value="1"/>
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        <location>
            <begin position="234"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="444" mass="51362" checksum="125C52577706CD6D" modified="2011-06-28" version="1">MSEQENVDKKLQEEIKNAFPYADVVDWNEVYQRIIYRYSTPHGLQHVKEEMEKLEDEGEIIVHHIKPYNNPVKMQSLNGFPKTIPTTRLWNHKSCGQCGHIPGYPTSVFWIMNKLEIDYMDEPHQTSCTGWNYHASGASNPVALAGVYVRNMWRAYEIDYFPLIHCGTSFGHYKEVRNMIILHKEVREKLRPIMRKLGMDIVIPEEVVHYSEWLYTMSKKAAQHKKYDLSNIKVAVHTPCHVYKLVPEDTIYDPEVWQGRRPAAPTGTVQNFGAKIVDYSTWWDCCGFGFRHILTEREFSRSFALFKKVIPAVEEAHADVFVTSDTGCVTTLDKSQWAGKAHGFNYNLPVLSDAQFAAIAMGADPYKIGQIHWHATDVEGFLRKIGVPVDDYKEKFLQYLADLREGKAQPEYLYKPHRKIDFYLALPERVKWYKGESAQTAKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I8</accession>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F4B6I8"/>
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    <dbReference type="GeneID" id="10601231"/>
    <dbReference type="KEGG" id="aho:Ahos_1731"/>
    <dbReference type="eggNOG" id="arCOG14917">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792004_0_0_2"/>
    <dbReference type="OrthoDB" id="42274at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="144" mass="16070" checksum="84DB2F9DC31D099A" modified="2011-06-28" version="1">MEQQQGEYVIWKDYPLSSYRRNALIGTSIIGAIFLLTVILAVLGIIIIAVGVTTYILTRRANEYVVTNKRAMHIKFGKVLKQVDLSTPGLVVSTVNPRYYSSRVAGQHVVEDVVFLVNGVEVLRFNKTSKGDELIAKLHSMGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M8</accession>
    <name>F4B6M8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775354.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M8"/>
    <dbReference type="STRING" id="933801.Ahos_0550"/>
    <dbReference type="GeneID" id="10599993"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="15446" checksum="865207302467E9B6" modified="2011-06-28" version="1">MAETKLLKLLGNRITSGILGLSLLVSSIYLVASIKVNFYDLLYETLVYFNPYFLYIIGIPLGLERLIYGITGNKKFSDFFFGRTEFTAMYLYFLSLFGIVMGIFIVIYSIALTGTLVKAMDIIDGVSFILFGISLVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N8</accession>
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            <fullName evidence="2">Transcriptional regulator, AbrB family</fullName>
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            <taxon>Thermoprotei</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
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        </source>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0560"/>
    <dbReference type="GeneID" id="10600003"/>
    <dbReference type="KEGG" id="aho:Ahos_0560"/>
    <dbReference type="eggNOG" id="arCOG00817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="30861at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF6">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="5"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="55" mass="6444" checksum="02EE04F27C2A8CE8" modified="2011-06-28" version="1">MADVEEIVKVSRNYQVTIPAKIRQKFQIKEGDLVKVVFDEKENAVKITLLKEPWK</sequence>
</entry>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R8"/>
    <dbReference type="STRING" id="933801.Ahos_0590"/>
    <dbReference type="GeneID" id="10600034"/>
    <dbReference type="KEGG" id="aho:Ahos_0590"/>
    <dbReference type="eggNOG" id="arCOG03770">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2857278_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="65" mass="7164" checksum="8141760D39862B8D" modified="2011-06-28" version="1">MAISSETKQPLVVKTLIKCMTCDYSIIRDFQQGDFVPKLVGTCPKDGGKLYIAGIYAESPSNQHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R9</accession>
    <name>F4B6R9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">AAA ATPase</fullName>
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    </protein>
    <gene>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="KEGG" id="aho:Ahos_1735"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="362" mass="42212" checksum="15370E954098CBB8" modified="2011-06-28" version="1">MDLVNSSSLSASAWEFIYLTEFNEKVKASSFNVLYAHKDEIGYTSFAKIREFPNWLRHLCIKKKWSEAFSYAERLYCMSYTKDVEGFIYFLRDLQNLGNVGLLLDEIKAEEGVLKELHKLINEIKLPITVSMTPDVMSKINDSALSRRLKERQIELKLSEEDKVDILRMYCEEYYEELLKIDEVAKASSVNELLDKARTAYELALEKCKEDYKKDECVRRELIKVFDIGNPMEVSKQLEAKIREGLLDLKSELKINYVHPKGKKIEEKGVIADIYFKTDNAVYIGDVKLSNRDNLDNIGNMKKLEDYDKEGTYEVKKFIITNQPNVNLENFKVIYVTNDEVKKIIEGDKEKINEIVRKIINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S9</accession>
    <name>F4B6S9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated protein, Csa2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1745</name>
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    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776537.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S9"/>
    <dbReference type="STRING" id="933801.Ahos_1745"/>
    <dbReference type="GeneID" id="10601245"/>
    <dbReference type="KEGG" id="aho:Ahos_1745"/>
    <dbReference type="eggNOG" id="arCOG03617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054331_0_0_2"/>
    <dbReference type="OrthoDB" id="97643at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002764">
        <property type="entry name" value="Cas7/Cst2/DevR_sub_I-a/Apern"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010154">
        <property type="entry name" value="CRISPR-assoc_Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052681">
        <property type="entry name" value="CRISPR-Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01875">
        <property type="entry name" value="cas_MJ0381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02583">
        <property type="entry name" value="DevR_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37459:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED PROTEIN CAS7_CST2_DEVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01905">
        <property type="entry name" value="DevR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="322" mass="35662" checksum="79E36FF0AFA9B753" modified="2011-06-28" version="1">MISGSARFLINVESLNGVESVGNLTKHRTAPVVVKTSTGYLIRYVPVISGESLAHAYQASLVDIAKSMNLPVGLYSSQYEFIKYSSDEVLKEEGISAPSSSNDVRRFEVEVLLKDIVSDVGGFMYAGKYPVRRTSRIKFGYMIPALTGEELPAQLEAQFHVRYSSKVEERQAIFNVEVSSALYTLTFSLDDDLIAVPSTIGNEVEGEEELEGQKTDRVKAAIKSLYSILTGNFGGKRSRFLPSMKLMSMVVTVTDFPFIPEPGHTDDYIKVSVERLNKAKSIFNSKNVEVFTINNENIEVPSNVKTLSSAEDLIDELIKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T5</accession>
    <name>F4B6T5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="10">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1751</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T5"/>
    <dbReference type="STRING" id="933801.Ahos_1751"/>
    <dbReference type="KEGG" id="aho:Ahos_1751"/>
    <dbReference type="eggNOG" id="arCOG00786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126922_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF83" evidence="11">
        <location>
            <begin position="54"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="151" mass="17679" checksum="A666E0F54012EEA1" modified="2011-06-28" version="1">MASVTDLKDFMLCPVIPWIRKKLGWREPITEGQRIAKNVKPRELANDLPDPKYYEVYMRDKSTGLSGIIDVLARDIVAEIKAFNRRFYGHFRIQLLSYAYLAERNGFRAKDAILILGREKRLKIEVRKEHIEYVEKITNKLVESLEEDSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T9</accession>
    <name>F4B6T9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776547.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T9"/>
    <dbReference type="GeneID" id="10601255"/>
    <dbReference type="KEGG" id="aho:Ahos_1755"/>
    <dbReference type="eggNOG" id="arCOG08454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392715_0_0_2"/>
    <dbReference type="OrthoDB" id="36645at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="11316" checksum="00BFFBDF435B1ACA" modified="2011-06-28" version="1">MNEIVVHTHIIGQKHMFVKITEFINTLSRKYHFNYDLYLHPITPSRDYVRTYSIPYEHFSKEGKWIISEEELLNVFEKELARIKKENNVNKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U2</accession>
    <name>F4B6U2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DEAD-like helicase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776550.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U2"/>
    <dbReference type="STRING" id="933801.Ahos_1758"/>
    <dbReference type="GeneID" id="10601258"/>
    <dbReference type="KEGG" id="aho:Ahos_1758"/>
    <dbReference type="eggNOG" id="arCOG00871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1003255_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="26"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="277" mass="31886" checksum="F008416148912CF4" modified="2011-06-28" version="1">MSEFIHPALDKEYNINGEVSSSLVTETGNSIPYGVLLADETGLGKTVIISLYLLYLKIRGLANNVLIASPKSITYQWRKELLDKASLKFKVINSGRDFEKINNNKFNIIVSIDLLKTTKGIQFIDKLNNYQIDVAVIDEAHHVLSANDTLRRRMIIKVREKSKSLLLATATPFRGVKEEEFIMHLLGQEYIFIGRIKENIEDTDGNPIFKPRKSITVKIELNFRWNFIYDHLNKMIDRLNTENIIKLILKKRLASSVYSFLITYNHIIDSKILLQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B3</accession>
    <name>F4B7B3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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            <begin position="7"/>
            <end position="29"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="136" mass="15058" checksum="372F430AB5E83DA2" modified="2011-06-28" version="1">MKGISDFVAFLIILVIIVGIILPLGLFLLNPYYQSQQSEPQNPQIINNGLITITYVSNNKGGIVNITYSTVEPEVIEIYNYSNGIWVKANYSFLSSCKNSLIYKVCGYAPEIDVELNIAGRIYYATVSYGSTAKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B9</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7B9"/>
    <dbReference type="STRING" id="933801.Ahos_1866"/>
    <dbReference type="GeneID" id="10601365"/>
    <dbReference type="KEGG" id="aho:Ahos_1866"/>
    <dbReference type="eggNOG" id="arCOG02441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="336" mass="37940" checksum="8EB6787AC9C8F7BE" modified="2011-06-28" version="1">MIVKHLRCEETSTLPVIHRVRFNISMFYNYRGLGQCFMAFIFGVKNNIGKLILVSGSNKGVANFTFYVKVYCTNSFHTRLYKVIKSCVINYSNYITISTIKVPARTVWIYVNATEEGPSSYYPIPRIEYIINKGLLSSSLIFTEFLSIIMIYLSYTFYARLIDSGSVEFIISRPITRAELYFIRLSSGILTAIVASAIFGSVFGLSFSLMIHCFAMYTVSILILMLLPILTFHYTLGFTLSNFIKSSTKTLGLSIVIFILITVINKILPILLEINTLSKALYSINPISISYVASYLLTHFTLAGAPFNIGISITVQLAWIIGLLVLGYLRFRKMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D8</accession>
    <name>F4B7D8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2173 domain-containing protein</fullName>
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    <gene>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7D8"/>
    <dbReference type="GeneID" id="10601384"/>
    <dbReference type="KEGG" id="aho:Ahos_1885"/>
    <dbReference type="eggNOG" id="arCOG05211">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170985_0_0_2"/>
    <dbReference type="OrthoDB" id="130444at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018685">
        <property type="entry name" value="DUF2173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09941">
        <property type="entry name" value="DUF2173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006821">
        <property type="entry name" value="UCP006821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12365" checksum="878005F5FBC88666" modified="2011-06-28" version="1">MSEEKLEKLMRIKGAVAAGQYKLTGELVAYKGEIPKEVAEMVAEMCAANTMMAKMQADGFSAFSKMNWKPLIGWAVAAGEYAVCVVGEYGVFVKLNEADFNEIFKTLREVAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I2</accession>
    <name>F4B7I2_ACIHW</name>
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        <recommendedName>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="STRING" id="933801.Ahos_0700"/>
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    <dbReference type="KEGG" id="aho:Ahos_0700"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36521" checksum="A3A0286B20C41240" modified="2011-06-28" version="1">MSSPSLAYISSISRLFKSTTLTKGLVTELLAENDWKNVLSILKERNFIEDIPATFDDAEIEIKRRAIDQIYKILNFSSSVKSAHDIVDLYYYYLTLDEFKSIIAGIYNKSRPSKIVFLSKISESNPTTIEELRSIIRGSIYSNALEYALSKNPRNLSQLESLLDFYFIDKLSSIIDTFKGDWKAAAEAIICGYKDYYSASLAIRQRIAIPLTCKILPDTIRDLQSSKMEEVANILRRTQYSRNIELGDAYSALYSLYRLARIEARKASIYAFMGSPFTPVTALAISELIKLDMEDLIMIINGLKIKMNKEAIKSRLSLEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I7</accession>
    <name>F4B7I7_ACIHW</name>
    <protein>
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            <fullName evidence="5">Ribosomal protein S25e</fullName>
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    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0705</name>
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    <organism evidence="5 6">
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775508.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7I7"/>
    <dbReference type="STRING" id="933801.Ahos_0705"/>
    <dbReference type="GeneID" id="10600158"/>
    <dbReference type="KEGG" id="aho:Ahos_0705"/>
    <dbReference type="eggNOG" id="arCOG04327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171557_0_0_2"/>
    <dbReference type="OrthoDB" id="43999at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="109" mass="12520" checksum="F1A880BF3BDA0CDE" modified="2011-06-28" version="1">MGGVSKKPISNIEKRLKRQQEEQQQKGKKKISKTGSEVISKNIQISDEMIKKIQEEIKREKLITPYELATKTNITLSVAKRILKDLNAQGVIKEVFRNRRTAVYINPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L3</accession>
    <name>F4B7L3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0731</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775534.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L3"/>
    <dbReference type="STRING" id="933801.Ahos_0731"/>
    <dbReference type="GeneID" id="10600185"/>
    <dbReference type="KEGG" id="aho:Ahos_0731"/>
    <dbReference type="eggNOG" id="arCOG00285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036272_0_0_2"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA central" evidence="6">
        <location>
            <begin position="229"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="553" mass="63346" checksum="DB199F02B949BA94" modified="2011-06-28" version="1">MQEIRHGIFLAIVILGLILPRLILAGYPLDSVYVSFGIFVSLFVDAILSYILFGSIIVSLVYFLSILSLIDILNYMNLVNIYILFPYSMGLAVALFFGMIIRRRYDDEFLSSLRKINITFNFYTILLSLTFLIFAIIIYFYIYNSQLFLIGSIIAFLLLLISGKKEDSPLILSSWIFLPYLMSQIGSNGEEKGICIGEIIGILKRSSFSSSKISTNSNYKWISYRSKFYINLERNKNFNIIIVGGSGSGKSHLAKNIIRKGNFHFLIFDIHGEYDVNAKRIDISKISINPLSLFGQSPRQRALEVAYMIKSIFNLGSLQTIDLSNIIVDAYGEKGIFEDDERTWNLPPPNFRDVLMLLEKKKKLVTTSQELNKIQSLEPYIYFLATNTFMDNSINLYDLISDDYVLDFSKVTIPEIKYVIIETILRSILAYMYISGQSNFRKMIVIDEAPFILSKESGEQLMERLFAESRKFGFGFILISQSVEYVRKLIPNTSYVFALNIVDPTEIDFISRLIGGQDPDIFKAIYNSLPKLERGLIITRDILRDEIILVRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T5</accession>
    <name>F4B7T5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776747.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T5"/>
    <dbReference type="STRING" id="933801.Ahos_1959"/>
    <dbReference type="GeneID" id="10601458"/>
    <dbReference type="KEGG" id="aho:Ahos_1959"/>
    <dbReference type="eggNOG" id="arCOG10433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923196_0_0_2"/>
    <dbReference type="OrthoDB" id="34709at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="321" mass="37799" checksum="F0C9F6057784E6A9" modified="2011-06-28" version="1">MDFLDEIHNVRYRLAMALKVSSFKIGDFTYIETSDPNKDFVIGEEEGKVFVLPINKDCIKFSQDIKHCLGFDYYYKEQFPWKEEVKIGVLDRIIRILKVFNDYDEIKNYIINQEIIQYGGKDYAMLEGDLYFSNPYEEFLDKLKDDDLENIKKLSKAYQEVKNFLDIQVENPTSIEDIYKEIESELIKIARKYNVELDYGRSIKLYSDERISEDEEHVEEEIREPINVNSLLAKIRAKKVIERKDEFLNFLLSKEGEFNIWGEIKGKGIILDEMKDKNVTVLLTSSQELEINIGGIKRKILVPKPSILFIKKDSAYFEIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B819</accession>
    <name>F4B819_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B819"/>
    <dbReference type="STRING" id="933801.Ahos_0810"/>
    <dbReference type="REBASE" id="35545">
        <property type="entry name" value="M.AhoW1ORF810P"/>
    </dbReference>
    <dbReference type="KEGG" id="aho:Ahos_0810"/>
    <dbReference type="eggNOG" id="arCOG04157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006958_2_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="323" mass="36614" checksum="0263FE264A4C5291" modified="2011-06-28" version="1">MELKVVDLFSGAGGFSLGFKNQGFNIALAIDINHAAARAYAQNFPSTVVLEEDIRNVTGEEIIDILGSSPDILIGSPPCEGFTAANPSRLKNPVDRLYLDERGSLTLEFIRLVGELKPKIFVMENVPAIVETKELKDALELEFSKIGYNQIYFNFLRAEDYGNPSRRLRVFISNIRINPPRLTKKVTVHDAISDLDGRTDVPNNEIHEISEKKMLKISKLGFGDYLTMYRGSKKEIPLYIRLDPFDLAPTVLGNSRFIHPYHDRFLTVREQARLMSYPDDHVFIGSKDEQYNQIGEAVPVALSTAIASYIKVKIREEYYSWFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B822</accession>
    <name>F4B822_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1974</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776761.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B822"/>
    <dbReference type="STRING" id="933801.Ahos_1974"/>
    <dbReference type="GeneID" id="10601473"/>
    <dbReference type="KEGG" id="aho:Ahos_1974"/>
    <dbReference type="eggNOG" id="arCOG01468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163753_0_0_2"/>
    <dbReference type="OrthoDB" id="42455at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051784">
        <property type="entry name" value="Nod_factor_ABC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43229:SF2">
        <property type="entry name" value="NODULATION PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="6">
        <location>
            <begin position="11"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="228" mass="25417" checksum="248FA91E12D54D21" modified="2011-06-28" version="1">MDLKTIKAYIPSILFFSLFFPFGLLIMLGSVASAEFSTYVVVGTITFYISVSLINSVAQSIGAEREYGRFSLMISSGIPVQLYALAIAFSNGLQTILVSPIIILAGDLFLRIKPDCIPLLAITLILSIFLGSMIGEALAFAIKNYYAINQYSNILSFTLAFFAPVYYPPCSVPMPFRYLTFLEPTTYISRAIYYSYVGIPKETLIWDLGIVIFTLVLIILINYLHKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B831</accession>
    <name>F4B831_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776770.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B831"/>
    <dbReference type="STRING" id="933801.Ahos_1983"/>
    <dbReference type="GeneID" id="10601482"/>
    <dbReference type="KEGG" id="aho:Ahos_1983"/>
    <dbReference type="eggNOG" id="arCOG02441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_527494_0_0_2"/>
    <dbReference type="OrthoDB" id="86287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50118" checksum="448F5CE9E22E943A" modified="2011-06-28" version="1">MNPIIYDFKRSFLRLSVLIFLIVFAAVGVGIGYLVYHSSVENLPASSYYNMNFVGVSFGFPNGTVKVHGYVFDNRGSPLVATIEMVSNSGKVVSMTQSNSSGFFTIQGNNASYIIVKYSTYEEKATITTIAAAGKSLLQQHTVMYTYIIYNVSDFYNYYEYNGEGLAIITGVKNCVGKLILASTFNQGELNLTFYNQDLKAVGSCIINYSGYVTINSIKIKPGTQFISLGSKSSTGFMTEYSPIPHIEYAINLGVISSSSLFQSFFPIIMIYLAYALYSKLIDSGAIEFIISRPLTRASFYLTRLTSGLLTGVTTGIIFSLVVGGTFSILIHYFPGFVVTMLIIELISILSFYYILSFMLSNFIKSATATLGISIALFFIIRIVEGILPIFLPTNVSNILGYYVNPTSICSVATYLSTHLTLAGAPFNLGISIVAQLAWLIGLSLLGYLKFRRMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B836</accession>
    <name>F4B836_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1988</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776775.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B836"/>
    <dbReference type="STRING" id="933801.Ahos_1988"/>
    <dbReference type="GeneID" id="10601487"/>
    <dbReference type="KEGG" id="aho:Ahos_1988"/>
    <dbReference type="eggNOG" id="arCOG01123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_2"/>
    <dbReference type="OrthoDB" id="45195at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
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        <location>
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        </location>
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    <feature type="binding site" evidence="2">
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        <ligand>
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        <ligand>
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        </location>
        <ligand>
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    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="365" mass="39936" checksum="4581E0A84DB1E8A1" modified="2011-06-28" version="1">MKVSLKELKENFNPKLKPIIWNDDKNTLTLLDQSALPFETTYVELKDPEGISSAIKLMKVRGAPAIGITAAYGMVTALTSDTPVTLEEAIEKLTKAKKVLDQARPTAVNLTWATSNMLNNAKKYVEQGEAKSVNELILLLKRDAKKIFDDELEAELQMGIYGLEKINDGDTILTQCNAGGLATGTGLGTALAPVKVAHMLGIKVSVIAPETRPWLQGSRLTVYELMTDGIPVTLIADTAVGLVMYKGMVNSVMVGADRILSDGHVFNKIGTFKEAVIAHELGIPFYALAPISTFDLKSRVEDVKIEERDPDEVRTIRGIPIAPEGVKVYNPVFDVTPPKYVTAIITEKGIIYPPFDKNVRKIVEK</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1991"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AEE94863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="22009" checksum="D6228B5D6616891C" modified="2011-06-28" version="1">MKSYFVFVDESYRPDIFALSVVWTKNKTEPCKIKNEALKKIRKEKYHFHFKNDDDEIRKNFMNTILKSSINFGIILTEYPSSCNEYARYYIAEVINNLPVEENSRVVITIKGINDWINKKKCKIYEEVIRWIRKRIFYTIKFNDKAGLEIADYIASAYTYCKFQKDNFCELLENKLTFLVRIR</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B884"/>
    <dbReference type="STRING" id="933801.Ahos_2037"/>
    <dbReference type="GeneID" id="10601537"/>
    <dbReference type="KEGG" id="aho:Ahos_2037"/>
    <dbReference type="eggNOG" id="arCOG05404">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2103361_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="114" mass="13657" checksum="06E2D953CB46BE35" modified="2011-06-28" version="1">MDLEKEIAYWRCHNKPVIFIAKTLNISCDEVRKVLNSWKEETRDFIFSLKEQDLGFFNPDITGLLKSSDLSVEYARKILSNKFVINYIVLNRERKHDRYMDCIRYHVKILLQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B889</accession>
    <name>F4B889_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FAD dependent oxidoreductase</fullName>
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    </protein>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B889"/>
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    <dbReference type="GeneID" id="10601542"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_651528_0_0_2"/>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
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        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="417" mass="47491" checksum="27DB7252AA7F3C26" modified="2011-06-28" version="1">MRAIIIGAGHNGLVASYYLRKAGFDVLIFDNKFGGMTDTTSIKGVKVSRASYVLGLMPRKFIDEFEIPIIEQDPIQTIWLEDRIIPFWRDKEKRMKELVNAGEDKFPEFEEKIFKFKKLLEEKFTFVTAPPSEKEVIEEAKNAGIEDVMRPSKEFLSKYLSRDLHDFFIYPGMEDSPAYLVAYFYDDWSFVKGGMGTVAEKIFEKANKLGVNFVSEKVNKILVEHDKAVGVKVGDKVYKADVILSTASPTETMKLAEYDHKFTLGKNKWIKYNVIFKEFPKVDERIKNFINSIIDFEGGEVVIPSTVDDRGGIVMEFMGNYEEVIEKFKGEIVYVDKLNARIAEEIYNLPAGNLNHLPMREPYLFDGRPEKGWGYKTPIKDLYLGGAGTYPGGQVSGIPGYNSARLIIKEKLGKDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B890</accession>
    <name>F4B890_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">inosine/xanthosine triphosphatase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_2043</name>
    </gene>
    <organism evidence="13 14">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>XTP + H2O = XDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776829.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B890"/>
    <dbReference type="STRING" id="933801.Ahos_2043"/>
    <dbReference type="GeneID" id="10601543"/>
    <dbReference type="KEGG" id="aho:Ahos_2043"/>
    <dbReference type="eggNOG" id="arCOG01221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087417_0_0_2"/>
    <dbReference type="OrthoDB" id="52857at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050299">
        <property type="entry name" value="YjjX_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699:SF2">
        <property type="entry name" value="NON-CANONICAL PURINE NTP PHOSPHATASE_PRRC1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-canonical purine NTP phosphatase/PRRC1" evidence="12">
        <location>
            <begin position="6"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="20127" checksum="2B04E1547A49EAFE" modified="2011-06-28" version="1">MKFYLGTTSKLKLSAVEEVLKNYVTDYEFLAFNSHSGVPITPWNEEIIKGARNRAENLRKKFLDNDGVYVGLESGLVERFGSVYEETWCVIIFREKEFSAYSSGLRLPSEIVEKIRQGIPHSDIMDEISRKFNSSPKDTWGLYTKFKLTRKCEIKEAFRNALVMMLTYEQASII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B7</accession>
    <name>F4B8B7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exosome complex component Rrp4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rrp4</name>
        <name evidence="7" type="ordered locus">Ahos_0830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775632.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B7"/>
    <dbReference type="STRING" id="933801.Ahos_0830"/>
    <dbReference type="GeneID" id="10600298"/>
    <dbReference type="KEGG" id="aho:Ahos_0830"/>
    <dbReference type="eggNOG" id="arCOG00678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071769_0_0_2"/>
    <dbReference type="OrthoDB" id="35160at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22524">
        <property type="entry name" value="KH-I_Rrp4_prokar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00623">
        <property type="entry name" value="Exosome_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023474">
        <property type="entry name" value="Rrp4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054371">
        <property type="entry name" value="RRP4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003181">
        <property type="entry name" value="PRK04163.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22625">
        <property type="entry name" value="ECR1_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="75"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="27747" checksum="9689B4C95EAD4D99" modified="2011-06-28" version="1">MDSNQNSNNKIYFKSRSIVVPGDLIAEGSFQIPWSPYIYKLGNKYYSTVIGIVDPKDSTFEIIPLEGSHYYPRVGDTVIGLIEDVELYGWIVDIKAPYSAYLPALSLLGRPVNIGEDLRKYLDIGDYVIARVESFDRTINPVLTVKGKGLGRLNNGKIIDIMPVKVPRVIGKNKSMYELLTSETGCEMIVAQNGRIWANCPSKDMEEVLFLSIKTIERESHIKGLTDRIKELIEKKKGELNVTNSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D3</accession>
    <name>F4B8D3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl23</name>
        <name evidence="8" type="ordered locus">Ahos_0846</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775648.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D3"/>
    <dbReference type="STRING" id="933801.Ahos_0846"/>
    <dbReference type="GeneID" id="10600314"/>
    <dbReference type="KEGG" id="aho:Ahos_0846"/>
    <dbReference type="eggNOG" id="arCOG04072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="OrthoDB" id="7751at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000532">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="82" mass="9098" checksum="6E87D54D20D4506B" modified="2011-06-28" version="1">MKIKEVLATEKATKLIDSENTIVIIVDRNTTKSEIKKEIEKALNVKVVKVNTLITPTGDKKAYVKLSPEYKASDVAQKLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K3</accession>
    <name>F4B8K3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF123 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776870.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8K3"/>
    <dbReference type="STRING" id="933801.Ahos_2084"/>
    <dbReference type="GeneID" id="10601584"/>
    <dbReference type="KEGG" id="aho:Ahos_2084"/>
    <dbReference type="eggNOG" id="arCOG00463">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115699_2_0_2"/>
    <dbReference type="OrthoDB" id="17296at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460:SF1">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14584" checksum="92869E68D0BDC02F" modified="2011-06-28" version="1">MIKYKGYVVFFYCDKDVNIIVNSRKEFELKKGYYAYVGSCGLHCDKRISRHLSGEEKIKKHWHVDYISSICIPLGVIVLPLSEKEIVKRLSSLRGIKGFGNTDDKTSPTHLFYSSIEEVMKRIFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L1</accession>
    <name>F4B8L1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type I phosphodiesterase/nucleotide pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776878.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L1"/>
    <dbReference type="STRING" id="933801.Ahos_2092"/>
    <dbReference type="GeneID" id="10601592"/>
    <dbReference type="KEGG" id="aho:Ahos_2092"/>
    <dbReference type="eggNOG" id="arCOG01378">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039939_1_0_2"/>
    <dbReference type="OrthoDB" id="33550at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="357" mass="40072" checksum="8E01E0B8F72A4DED" modified="2011-06-28" version="1">MIYPDYSKNNLYNVACSIEDALVGGKACCSNITLSDKIFLVLVDGMGYNILKSAVEGIKFDKIYTVFPSTTATALTTLMTATKPGEHKILGYTTYVDKVGVINALKYTMPETSEVDLLKKALGKSMEEVFNVKSIGKKAREKGKKSVSILPKCIAGSEFTKMLYTNVEEYRTLWDGIYKAKIFGEKGVNFITLYIPDVDLTAHRYGPYAEPTLRAVKDIISMILRDIPKGYDIIITADHGFIQTENAIMLDEDKDFVSSLDSPPFGDARAIFMRSRKDLKCLEEKFSSFKVFTIDEVKELGLLAEKGEKIDIHADYLGIPTDRKVYVYRFKNGYAGHKGHHGAMLEEEMEIPLIRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L7</accession>
    <name>F4B8L7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3834 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776884.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L7"/>
    <dbReference type="STRING" id="933801.Ahos_2098"/>
    <dbReference type="GeneID" id="10601598"/>
    <dbReference type="KEGG" id="aho:Ahos_2098"/>
    <dbReference type="eggNOG" id="arCOG03692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243094_0_0_2"/>
    <dbReference type="OrthoDB" id="56755at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024533">
        <property type="entry name" value="DUF3834"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12916">
        <property type="entry name" value="DUF3834"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23078" checksum="0BBC6010DA18FAAF" modified="2011-06-28" version="1">MVTVLTPPGPVAYPIIASTMKRRDVKVIFEGNAKVKLNAIPLLNKVNYVLVSRMLVITPGLGKKIAVWKRGGANHILLDIVLKLYNHKAELVFTDDPAEVYKLYKEGKADSAVVTTAVTKDGLYFEDLLSAKGIYLPGICGAEGLNEDFETAYLEGIDLFKEDPEGTSEYVADNLPIYRPSTFIESIFKNSEYNLRKLDKPYVFRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M9</accession>
    <name>F4B8M9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF763 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="380" mass="42765" checksum="84E12CDDA549FD10" modified="2011-06-28" version="1">MEIEGIADLPLHTGHVPSWLIPIMRKLSRAILDIMLIEWGPEKVVERLSNPLWFQAFNNVIGMDWDSSGSTTVTLGILKEVVNPKDDGLAVLGGKGKNALKVPEELHSLNFDIDADKLANISRLVAKVDTTLVQDGHQLYHHSMLVTEKGYWGIVQQGMNEETKFARRYHWKNTENFTVDPHEAIAGKKGIAVNIIEKQKENTRKLVLDLLRQDPRKIINELQQAKAILKGQATIESWINGASFVGISKEAKIIYMRPLDERKIKPILEKLYESNPENLEEALLEGLGPSTAKALYLISDLIYREPPSYNDPVNIPYDPFKYAYAIGGKDGIPYPVNRRVAEEVIITLEDIIQKAKLESNEKKFSLNKLRGLSIGIKEGS</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_2123"/>
    <dbReference type="KEGG" id="aho:Ahos_2123"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_439172_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
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        <location>
            <begin position="170"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
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            <dbReference type="PROSITE" id="PS51194"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="622" mass="71032" checksum="64D3D6ED6F455A00" modified="2011-06-28" version="1">MRAKGISVYVSPLKALSREVYEDIRDKRDTKYVDSDVYEDDLRSFKSKTLLSTYEKFDSSIRHKYGWLRKVEVVVIDEIHNVESDRGLAIENIVLWAKKNGVQIIGLSATLPSVKNYANWLKAEVIEYNKRTVPLHECIAYPYVIRCYDNDFTIPLEPLNGIRNEKLEILIPALRYIIKQGKNALVFVRSRKSAESLAETLRKFGFNASHYHSGVPLEERRQIVEMLKKGEINVVVSTTALGQGVNLPVYATIFYDTVLPESDEKGNLKGWRDLNLMEFKQMAGRAGRPGFDKEGMSIIISTSMRKAEEFRRKYFSSSYEEEKSTYSLDDLSLGVISWREGFSKEDLQSILKCSLKFNKITEEEFLKSLNALKEVNLIEEDGGGIFLTPLGKAVSLSYIDISLLKGFQIISSDLLTTVVSSNAVAQELRGCKCGRELLKRWSNGEDFSSLCKNLSAKDINEVISNSRWISFALYRVLKALGKNDEAKEALKLYYQIKYGVPFEGVRLAKLGLDRGTVMKLLVDKKIKDEKDLCIKIFTPPLKILMKERGYDPAGVCKEVYSKDLDVYEMARVVIKYYGKEFEGKKIDKEILKKLISLGIVEKKVRKEKDGNEKIVYFIQPFS</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="15344" checksum="90C814A849A0C047" modified="2011-06-28" version="1">MIEEEIYNKCKKDWNCASSIISSLPFEEDTKKRIMESYVEKFVGKRIFLVQLVTSMIYQCGELNSKKDEINCYLSTYYSGRVEIPLKENSLILLHSIFRNIIKDNHEEDLLDMCKQGNELACNFIEEVSLI</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <strain evidence="1 2">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="GO" id="GO:0008410">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF57">
        <property type="entry name" value="SUCCINYL-COA:3-KETOACID COENZYME A TRANSFERASE SUBUNIT B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="227" mass="25356" checksum="A71406A5399A6EFB" modified="2011-06-28" version="1">MSIHRNPMGFIFELVKSGIRNLNFVDREPGLGLEVLLQNNVLKKVRIAMATLEWFGMLPSFRMKAESGEIEILEDTCGAFIAGIRAGAFGVPFMPVKGIIGSDLIKLHEKEGTWKIVDDPFSGEKIVLVKAITPDVAIIHVNKADPEGNAEIEGPVYEDEYKARASKKVIITTEELVDKSYFYKKRPNIYAEHVTAVVHMPRGAEPTSMFPLYDADYEKILNILGMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U1</accession>
    <name>F4B8U1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775727.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U1"/>
    <dbReference type="STRING" id="933801.Ahos_0925"/>
    <dbReference type="GeneID" id="10600397"/>
    <dbReference type="KEGG" id="aho:Ahos_0925"/>
    <dbReference type="eggNOG" id="arCOG01143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004962_0_10_2"/>
    <dbReference type="OrthoDB" id="303721at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00144">
        <property type="entry name" value="MPP_PPP_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF496">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="1">
        <location>
            <begin position="98"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="30352" checksum="C62CB0433F99234E" modified="2011-06-28" version="1">MALSESEINEILKIINEAHDFFISEPLLLELPEKDTVAFVGDTHGALDVTEEVFRELYDKVDILIFLGDYVDRGSHGIENLTLILRKMLENPKKVIVLRGNHESPLTNEFYGFKGEVLEKMGDYYEDFVNMFSVMPYAAIINGYFCVHGGIARNLEDVKQINSLRRPDVNPDDEIAFEMLWNDPREELEGFVPNVRGEGTYFYGKDVTLKFLERNNLKGIIRGHEAVDAFRFEMDNRVITVFSSRYHGLGAGALVLNKGTFCKVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Y9</accession>
    <name>F4B8Y9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">prf1</name>
        <name evidence="11" type="ordered locus">Ahos_2141</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776927.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Y9"/>
    <dbReference type="STRING" id="933801.Ahos_2141"/>
    <dbReference type="GeneID" id="10601644"/>
    <dbReference type="KEGG" id="aho:Ahos_2141"/>
    <dbReference type="eggNOG" id="arCOG01742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="OrthoDB" id="1011at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE95012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="354" mass="40627" checksum="0B1C8E8C63B865B1" modified="2011-06-28" version="1">MKVLLKELKKWSAPATVLLSLYIPPGRPIADVLNNLRQEASISQNIKLKRTRDAVESAISAAIDRLTSIPKVPDNGLVLFCGENFETEEFKCYMFSPPEKVTVYFYRTDKFFHLEFLEDMVEENEVYGLIIVERDTATIGILRGTRIEELEEIEGYVPGKHMMGGQSQRRIDRIIEELYHDFLKEVGEKVNSYFLPYLEEKKLKGILVGGPGYAKNDFYEGDYMEYRLKKLVLEPLIDVGDQGEAGLREMVMKAKDLLKNQKYVEVENLLDEIKYHLAKDDGLVVYGKEEIKKAMDMGALDALIVHEDNEEDSLTKEAEKFGTKVYVINDEVPEAEWIKKTFGGSIGKLRFRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z5</accession>
    <name>F4B8Z5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776933.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z5"/>
    <dbReference type="STRING" id="933801.Ahos_2147"/>
    <dbReference type="GeneID" id="10601650"/>
    <dbReference type="KEGG" id="aho:Ahos_2147"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="OrthoDB" id="14763at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154">
        <property type="entry name" value="LEUCINE-RESPONSIVE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30154:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR AZLB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17659" checksum="18FFE13ECB8DC26D" modified="2011-06-28" version="1">MSEHYYLDDVDRKIISILQQDSRISFSRLAKMLNLSESTIHMRIKRLREAGVIRGFCVDVDLDKVGMNVLAFVLLKADPKKYEDILRKLAEIKEVFEIYDVTGEYYALLKVRVSDKEELAKVLDKIGNMEGVTSTYTMFVLRVIKEKKNAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B913</accession>
    <name>F4B913_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PqqD family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776951.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B913"/>
    <dbReference type="STRING" id="933801.Ahos_2165"/>
    <dbReference type="GeneID" id="10601668"/>
    <dbReference type="KEGG" id="aho:Ahos_2165"/>
    <dbReference type="eggNOG" id="arCOG03838">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166552_0_0_2"/>
    <dbReference type="OrthoDB" id="211309at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1150">
        <property type="entry name" value="Coenzyme PQQ synthesis protein D (PqqD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008792">
        <property type="entry name" value="PQQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041881">
        <property type="entry name" value="PqqD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05402">
        <property type="entry name" value="PqqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10571" checksum="BBB7E910B40FD5CE" modified="2011-06-28" version="1">MNFDEIKDKKPQKVGEFIDKAEDGDNYIIKVSDDKIFEVAPIAYYIWAMCDGSKTVTQIIDEISKEANIEFDQLKDPVTAVLEQLQQASLITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B943</accession>
    <name>F4B943_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776981.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B943"/>
    <dbReference type="STRING" id="933801.Ahos_2195"/>
    <dbReference type="GeneID" id="10601698"/>
    <dbReference type="KEGG" id="aho:Ahos_2195"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43613">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43613:SF1">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="8"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="243" mass="27299" checksum="71B6EA4C724677CE" modified="2011-06-28" version="1">MTMEEIAVSVTNLRKTFGNKEILKGLTFKVPKNIIFGIIGPNGAGKTTTLRVLAGIIRHYQGEVKILGLPPTKAREKGYISYMPEDAFPYDRLTGLENLEFYAKIYAKNKDEFEEFLEKGIKIADLGDRIYDKTQEYSRGMKRRLMIARTLMVMPKVAILDEPTSALDVESSVKIRKLIREMKGETTIILSSHNMLEVEYLCDEIVLLNHGEIIASGTPKEIVNKLGVKNLEEAFLVATGGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B978</accession>
    <name>F4B978_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved pro-fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775787.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B978"/>
    <dbReference type="GeneID" id="10600458"/>
    <dbReference type="KEGG" id="aho:Ahos_0985"/>
    <dbReference type="eggNOG" id="arCOG12065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893115_0_0_2"/>
    <dbReference type="OrthoDB" id="384429at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="275" mass="32358" checksum="C989DE3DC93CF7AA" modified="2011-06-28" version="1">MAQPIINNDFANTSLSLRDTLDASNETHTNNLLHRERIWELLKRNRELREQVEILDLLERIREEGLVGNDEIKFRVSASFKELYNKYYAHRKPTLSPKTQVIREAIFRIILDDLILSGKIDTETLRQFGFEIPNLEEVRKAERVVYMPIIQKEEPKKENSEKESKEKELKDEFKQKFKEFEKFLNELIFTPYNLINNAYLVTKPLAKKLFQYIDELVNMDSTPPEAKQLLIEMKGYLAMYPLAERNFKNGIASIPTGVHTQEITPRLTKIEKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B982</accession>
    <name>F4B982_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved Sulfolobales protein (DUF929)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="entry name" value="CELL SURFACE PROTEIN"/>
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            <begin position="353"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="422"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="726" mass="79759" checksum="F83E605BD106D4C4" modified="2011-06-28" version="1">MAILVGVASSAYLLSQTHPTTAQPNYLKNAQWVPPSAVKPGEWVKVTNQDFAPVNKTEVFLDGWIGCHVAAMDSWLIFYVLSHYGFNFTYEFHTSDPYRTPPNVPGLIFEGYKAPKNSPIIFDWIYMYNQYLNEALLPTPQPMNYSISNTIAIYYGLMELKEFAPQWVYNIAVTYNVPIVGQYKSPPHVVTMLIVTGPNGTWLLLGPSYPPSAIDKLTPEEVMEELITQSNPELMKAIHQFWQILNESMGKPSVAVSVCVQAEEITSYLHGFGFTTYYYPCNYPKEATGPSCWIMYANSECRNPVFSGDLNTSWIYQQMNAFQYGNITAWEEDIKLLGSEFGSGYEQAAGDAVGPSFANGIVYVGSDSGQLYAINAYTGKAVWILTTPAAVIMSEPVVYHGLVYVGLGPATFTYEQGKLNAYGGGHRGLYTGLTGLLAVNASTGVPKWLFLTKSQAMPTPVVYNGMVIFDDGDGNVYALNATNGQLIWNYSYDGSANMASLNLYIGKGYALVITAFSPGYPVNMSAIIALYANNGTPAYIIKVPFAYGSSVGDAVPAIYGCYLVDSFMGYPIYHGVYLNQIYVRQVLLVANVTNGKIIYVKNITGYGHPGDDNNGFSPLVYNGIIYVPSHINRTVAAFNLSTGKLIWVSPKLYHASLADQPVYYDGYIIVPDFDHIAVLNATTGQLVNKYYVGVDLGKDQPVIVGNDLIDSSIFGYIIAVPLCLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B983</accession>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775792.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B983"/>
    <dbReference type="GeneID" id="10600463"/>
    <dbReference type="KEGG" id="aho:Ahos_0990"/>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137177_0_0_2"/>
    <dbReference type="OrthoDB" id="55635at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14657" checksum="D41C4EB6E34E0F2A" modified="2011-06-28" version="1">MGVFKKEKIYPGQHNLQTIIQEFTQYLQNDGWKVQQKTEDNIAIIQAQKGGILRDIFAADRALQFSFEQTPEGLKVTVGIGKWVQNLAVTAVEAIIYLPLLAVDIPEMLWTQHVESKLMKELDRIVSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A1</accession>
    <name>F4B9A1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1008</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775810.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A1"/>
    <dbReference type="STRING" id="933801.Ahos_1008"/>
    <dbReference type="GeneID" id="10600481"/>
    <dbReference type="KEGG" id="aho:Ahos_1008"/>
    <dbReference type="eggNOG" id="arCOG02692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="7"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="392" mass="42354" checksum="F96D00C193EF37F2" modified="2011-06-28" version="1">MQKYIHATISAFFSWAGNIYDLLIVTYVYYYFQKCLGLNAVEGTLLFALGLIFRVIGGYVFGKIADQRGRKPILAIGTAGYSLFQGLMAFSPNAVILLIARAFQGLFMGAQWTAGTVIAYEQAPPSARGLINGIVQAGYGVGYALTGVAFIAFSQSMGGIGWRLFLLTGSIPIILLPYIILKVSDVKVSRKAISNANVKEYTKILIRASIIISGMFFSYYSIFAVYPGLAEYLGLSKDFVGLMMTVANVSLAISFIVYGRVADFINKRKLITYGVIGEMIGLPMMLPIILKSPELMLIGLLVYSIATGFWPLAPLLIVESVPQEVRSALTGLSYNLGSVVGGVGSITMGTLVQIFGLANSPLFGNILGYSSLLIVLITLLTWPRGTVIAKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B7</accession>
    <name>F4B9B7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1024</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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    </dbReference>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="16196" checksum="CF41EEF65C871D9A" modified="2011-06-28" version="1">MINFSDFYENKIPLLQVELECPKIKRGKIVLGLIDTGSRYTIINKHVVEECFSEEELKTRYFDSVIINNKKYERYSFRFTFPQLDNIGYIFNAAIIEYDPFPEELVPNVILGREDFLRDLIVCLYKSELITIYKEF</sequence>
</entry>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C4"/>
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    <dbReference type="GeneID" id="10600506"/>
    <dbReference type="KEGG" id="aho:Ahos_1031"/>
    <dbReference type="eggNOG" id="arCOG02431">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107614_0_1_2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MCP/YpsA-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="172" mass="19300" checksum="87F627456CC4B584" modified="2011-06-28" version="1">MQLAIAVHSSLNKELEDKIKRFFENLKCNPTILVGGYWGGMKVVVDEAIMKKLKVVVILPEEREDVELPPDVIRINSGCEYRCRSVILVRSADLLVSLGGEVGTTIEIFMAYAMGKRALVLSDTGFSTDKLKTAFSEYLDNRKVMKVEYYSSPEDLAKEVCKPITQIKTNFG</sequence>
</entry>
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    <accession>F4B9D3</accession>
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        <submittedName>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D3"/>
    <dbReference type="STRING" id="933801.Ahos_2208"/>
    <dbReference type="GeneID" id="10601713"/>
    <dbReference type="KEGG" id="aho:Ahos_2208"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156995_0_0_2"/>
    <dbReference type="OrthoDB" id="34441at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="AEE95079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="15374" checksum="A54734CD6E030CB0" modified="2011-06-28" version="1">MIISVPDVIYLHSGEYTISISVLPTKTEGEVIEILKSTMRISECKDKLLCYPSIFGGIFIFLRKSILSRIEFDGYLCQGKDGELFDVNKFHENLKHDFSCFRFDDGKIYCFSRARKDKECKPIDNIGLRFIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H0</accession>
    <name>F4B9H0_ACIHW</name>
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        <submittedName>
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        <name evidence="1" type="ordered locus">Ahos_2245</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H0"/>
    <dbReference type="GeneID" id="10601751"/>
    <dbReference type="KEGG" id="aho:Ahos_2245"/>
    <dbReference type="eggNOG" id="arCOG05910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324071_0_0_2"/>
    <dbReference type="OrthoDB" id="43856at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="23098" checksum="9AB5C88AB1C09BA0" modified="2011-06-28" version="1">MKFFKPVKSFKDVNVAIEVFAELSASRLSKIMGFPLLDLKITEKEGKIGLLMDHLPEKADENAINIDKIRSALAFEEVILNIDLKEEHVLSKEKIAYIIDHGHSFNAWKPLYYIQEIIDKKVSRFNLWSNREKFMEGVEIINSVDDKNILEILRDTAEEVYQYNFCKLFDRNAMEETIDLSFRIFRYRKRILPSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M8</accession>
    <name>F4B9M8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1060</name>
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    <organism evidence="8 9">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775860.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M8"/>
    <dbReference type="STRING" id="933801.Ahos_1060"/>
    <dbReference type="GeneID" id="10600534"/>
    <dbReference type="KEGG" id="aho:Ahos_1060"/>
    <dbReference type="eggNOG" id="arCOG00492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_2"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000053">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="25"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="377" mass="42675" checksum="B99B7DDB4BF6B366" modified="2011-06-28" version="1">MVSRIGKEIEISPVELASQLAKKAKINLASGSPDPRVIPINEIREAYNEVLEDVKSLFYPGAGGQDELKKEIEEHYLQFLGLSKGEDEIVITSGAQHAMELIGKYFLENDVIAVENPTFIETFNAIKLRSSVVIPIDLDDKGIKVDQLEEFLKLVKINLLYVIPNCHNPAGVNLDEERRKYLVELAERYDFYILEDDPYRPIAGCVPKPIKYFDKSGRVIYISSFSKILAPGLRIGFILANKEVAQKISLLEQLDFSTSTVNQYVVARLIRKGIVTRRMKELYEHYSRKMKVLKESLTDQGLTKFNDPKCGFFLLLDLEKDSWKVFNNAIQLGLSFVPAKPFFLRGGDTMARLSITMSSEEEIKEGVSILKKAVQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
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    <organism evidence="1 2">
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X2"/>
    <dbReference type="STRING" id="933801.Ahos_2317"/>
    <dbReference type="GeneID" id="10601825"/>
    <dbReference type="KEGG" id="aho:Ahos_2317"/>
    <dbReference type="eggNOG" id="arCOG04171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135994_0_0_2"/>
    <dbReference type="OrthoDB" id="9265at2157"/>
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        <property type="entry name" value="mth639 domain like"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
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    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696:SF1">
        <property type="entry name" value="DUF371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="DUF371 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17534" checksum="F85B615A8E21A959" modified="2011-06-28" version="1">MSKLVVDTLEIYGHPNVLGKHKSTLEFTKENYLTPRGDCIIGINSTKAIADLKEEVKEILKENGYGYVVIKVNNLIDIVSGRGDKRMTFSNRVKIIIRKSNFISDSTLLVYSDKAAADIKRDIINKLRQGDKAKVFIIASDIPLKNEEILRVIVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y2</accession>
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        <submittedName>
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    </protein>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777113.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y2"/>
    <dbReference type="STRING" id="933801.Ahos_2327"/>
    <dbReference type="GeneID" id="10601835"/>
    <dbReference type="KEGG" id="aho:Ahos_2327"/>
    <dbReference type="eggNOG" id="arCOG05884">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165243_0_0_2"/>
    <dbReference type="OrthoDB" id="34556at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="13993" checksum="D9EAE35DF3D9A820" modified="2011-06-28" version="1">MFWEAYRKIEKGTEVSLEEFRSNDELKSEVKEGIIELYKEVISEARRLINEPDDEKLFLELFRRNIIDSYLLQELIDIMNIIKNLHKTDDDVIYGLLVRIMEDLEELFYSVKKFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA05</accession>
    <name>F4BA05_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777136.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA05"/>
    <dbReference type="STRING" id="933801.Ahos_2350"/>
    <dbReference type="GeneID" id="10601858"/>
    <dbReference type="KEGG" id="aho:Ahos_2350"/>
    <dbReference type="eggNOG" id="arCOG07274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2103613_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="114" mass="13463" checksum="B588657A8816F529" modified="2011-06-28" version="1">MKTKETIKQRKVRSNESISAFKEELRALSFEPIYGESVKDIIMRFTTKIQELAEQYGYEIDFPKKAEVETDGNIYYFVYNIKVKTKMSVKKLKISIQYIMYDNNQWVGLITGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA11</accession>
    <name>F4BA11_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1124</name>
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    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775924.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA11"/>
    <dbReference type="STRING" id="933801.Ahos_1124"/>
    <dbReference type="GeneID" id="10600598"/>
    <dbReference type="KEGG" id="aho:Ahos_1124"/>
    <dbReference type="eggNOG" id="arCOG00454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137725_0_0_2"/>
    <dbReference type="OrthoDB" id="42993at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053658">
        <property type="entry name" value="Cdv_Coordination_Protein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041009">
        <property type="entry name" value="cell_div_CdvB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="163" mass="18531" checksum="51F868853891B66D" modified="2011-06-28" version="1">MKKKRDIADVLTDIRIARNRLRIMKTKIEGRLTQQESLSRSAVLTKEYIKEAEQLKKISEFLDTLDIILELIEIKVETIIYIGYIVNDAPAVLEALRELKKNGEFLSPELSALVDDIYNGFYSAINVPSEIKVSASKEAKKVLDEAKTIAKYRESGKNIDINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA23</accession>
    <name>F4BA23_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1136</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA23"/>
    <dbReference type="STRING" id="933801.Ahos_1136"/>
    <dbReference type="KEGG" id="aho:Ahos_1136"/>
    <dbReference type="eggNOG" id="arCOG01997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_1_0_2"/>
    <dbReference type="OrthoDB" id="10856at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="201" mass="21563" checksum="0DD37D2E7C0D1F4B" modified="2011-06-28" version="1">MELYQTVIIAIKLFAIMDPFSIIPYLLAIFEEYSQSSDTKVSWNYLINKVELAVIILLVFFSLIGKAFLDFLGLTPASLEIGGGIILVYLGIDTMGGFQQLKFLGRSIVEAAVTPIATPLIVGPGTMAALVTLSVSFPVYYLLIGSLIAAGITYVVLRFGPLLVKILGRTGTVAAGRFTAIIIAAFGVQLILEGISQINLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA26</accession>
    <name>F4BA26_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775939.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA26"/>
    <dbReference type="STRING" id="933801.Ahos_1139"/>
    <dbReference type="GeneID" id="10600613"/>
    <dbReference type="KEGG" id="aho:Ahos_1139"/>
    <dbReference type="eggNOG" id="arCOG08337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127691_0_0_2"/>
    <dbReference type="OrthoDB" id="35615at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="113" mass="13582" checksum="E1FDAA5A11982807" modified="2011-06-28" version="1">MKISEVPNLYLLDLENMQIIIEELKTEKGEKIYSIYQNRKYNINEEKEWTPDVDKAKKIKEEELPAEVAKYLNKLGIYVKVPKVVEKKEQQKVETKEEKKQVEKKEEQKQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA58</accession>
    <name>F4BA58_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proline--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="7">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proS</name>
        <name evidence="9" type="ordered locus">Ahos_1172</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775971.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA58"/>
    <dbReference type="STRING" id="933801.Ahos_1172"/>
    <dbReference type="GeneID" id="10600646"/>
    <dbReference type="KEGG" id="aho:Ahos_1172"/>
    <dbReference type="eggNOG" id="arCOG00402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="OrthoDB" id="7375at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000037">
        <property type="entry name" value="Proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="41"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="481" mass="55691" checksum="6E91806299621B4E" modified="2011-06-28" version="1">MQIPREKWKNNFSEWFDNVIFQAEIYDYGRYPIKGMGVWMPYGFKIRQNVINIIRKFLDETGHEEVLFPLLIPEYLLRKESEHIKGFEGEVYWVTKGGEEDLDIRLALRPTSEVAITYMESLWIKSYKELPKKYYQIVSIFRYETKATRPMIRLREVTTFKEAHTLHVSYEDAERQVKEAIEIYKKIFDTLGIPYILSERPEWDRFAGAEHTYAFDTLMPDGRALQIGTVHHLGQHFTKALDYKIQKADGSLDYPYQTSYGISDRVIAVSIAVNGDDHGPILNPIIAPIKVVIIPIPAKTEEDNKRVENYCKEIQEKLSSAGISAVVDSDKEKTPGEKFYYWEMKGVPLRIEIGLREVNNETITIKRRDTLESKIVKKDELIKTVNDLLSSLSEDLKKKAWDFFNSRIFYTNDVNEAKKILENRGGIIEVPWCGDNSCGLKIEEELNARVLGYPIDAKKTSDPCVICKKPSTNVLRVAKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N9</accession>
    <name>F4B3N9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SelT/SelW/SelH family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774845.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N9"/>
    <dbReference type="STRING" id="933801.Ahos_0033"/>
    <dbReference type="GeneID" id="10599473"/>
    <dbReference type="KEGG" id="aho:Ahos_0033"/>
    <dbReference type="eggNOG" id="arCOG06207">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068510_5_2_2"/>
    <dbReference type="OrthoDB" id="33017at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9552" checksum="88C6095D84FEE786" modified="2011-06-28" version="1">MEIVKIVYCRPCGYLDMALELARDLLQYFENIKVEIEQGKNGIFDVYLNDELIFSRYKEKRFPENMEILKEVGKRINNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q0</accession>
    <name>F4B3Q0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776003.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q0"/>
    <dbReference type="STRING" id="933801.Ahos_1204"/>
    <dbReference type="GeneID" id="10600680"/>
    <dbReference type="KEGG" id="aho:Ahos_1204"/>
    <dbReference type="eggNOG" id="arCOG07278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441893_0_0_2"/>
    <dbReference type="OrthoDB" id="43116at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="474"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="618" mass="70894" checksum="69361EB8697B755A" modified="2011-06-28" version="1">MSPFIKLTTNYCNLYEKIILMILNSILVYILSLNLHFIYNYNLEIISVVAVISFFLPEIVSPALTILFTIYLAYTELNLNQLSGTIEIISIIILNILVPILIEIKYGSMQGFMSSEAIIGFPISSLLLLSGIAEKRNLTANVLSSLPLFFILFNHFDTIMYSTNLLFYYYILLGIISLVIASILFSLKQLISISGIIFSFIGLSTLFYLTPLPHPIPLNLIYTIIVAASVNAIFTGFYELKIRKQMKEKIQEELSLIKKEIDSSIISLGRIRSYAELEDSLSNIIAEDEKSILEISKKADQCKSLDCINSIYNEFISAKKNIEDKLSQYIFNTVIEYNNVIKELKKNGIILEEISIPSEKIMLSEDDIDKIQKILSTINKNISVGVSEINSIIDSIEKISGIKLNRFYITEYSSIVSAIDYLKKINVLTYVNQCVSYDRDILTKLEFYGFENRKLEIARKLNEYYGKEILLSDIKNIERESNQLLIIMNEYLNNIKNELEKIWKISKLSNIKNKIEVIDSLINELNKDDAILKKLSDVLTAIPEISNAEKIIEEKDNIYALFTILRENEDIIREKLNEEQCIELEELGINSKLSFYVIEYLKERNINVKLDTNKICLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R0</accession>
    <name>F4B3R0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name evidence="4" type="ordered locus">Ahos_1214</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776013.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R0"/>
    <dbReference type="STRING" id="933801.Ahos_1214"/>
    <dbReference type="GeneID" id="10600690"/>
    <dbReference type="KEGG" id="aho:Ahos_1214"/>
    <dbReference type="eggNOG" id="arCOG01033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_4_1_2"/>
    <dbReference type="OrthoDB" id="8744at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="3">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="2">
        <location>
            <begin position="118"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="124"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="262" mass="29261" checksum="36D8A14433ABB917" modified="2011-06-28" version="1">MFIDYSTKLFGIIGKKISYTLSPAIHNYSFEKLGINAVYLAFDIEEEKFDLIVKGLLEVGEGFNVTIPYKEKIIPLLEGLSKEAEEIGAINTIFRKKGFNTDYLAVKSLVLEKGEKIEKSLIFGAGGAAKAASFALSSLGSEIFIINRTRDKAQELVNRLTERGYYAKVVESCNFSYDAVVNSTPNPSYIPGECIKGKLAIEFVYSPLNTEFLIKASKKGLRVINGLEILVRQALEAQKIWFGKSLNDKEVVDFLYARKLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R9</accession>
    <name>F4B3R9_ACIHW</name>
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        <recommendedName>
            <fullName evidence="4">KEOPS complex Pcc1-like subunit</fullName>
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        <name evidence="2" type="ordered locus">Ahos_1223</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R9"/>
    <dbReference type="STRING" id="933801.Ahos_1223"/>
    <dbReference type="GeneID" id="10600699"/>
    <dbReference type="KEGG" id="aho:Ahos_1223"/>
    <dbReference type="eggNOG" id="arCOG01354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191159_0_0_2"/>
    <dbReference type="OrthoDB" id="27208at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9595" checksum="A35FE7E85790BD48" modified="2011-06-28" version="1">MKAEINIQIQSPHAKEIINSLKVDNINIPQGMQIDMNYNENFANIKIIMEISSPKDILTLRNTADEILEHANLIINLLENKRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B404</accession>
    <name>F4B404_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-keto-4-pentenoate hydratase</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0075</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B404"/>
    <dbReference type="STRING" id="933801.Ahos_0075"/>
    <dbReference type="KEGG" id="aho:Ahos_0075"/>
    <dbReference type="eggNOG" id="arCOG06004">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369583_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23194" checksum="8A6C7C77F8CD4CF8" modified="2011-06-28" version="1">MAFNFFLIMEKAEQLFQAYKERKEIDPFPLTEEEGKKVGEEFSKMLIDYEGLGGYKIAKGGIIGVLTKAMITSSEDVELWFKTHKVEVELIALVKSGKISKIYLGLEIPATRFKTWDLPTQYIIADDAFAGRLFVGKEIEPPYGSFKLFINDKYIATGSPTYSPQSLIKDFMEGYVALGAFLGPYKISKGDKIRVEGKKTISVKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B434</accession>
    <name>F4B434_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B434"/>
    <dbReference type="STRING" id="933801.Ahos_0105"/>
    <dbReference type="KEGG" id="aho:Ahos_0105"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43335:SF4">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE92998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="305" mass="34337" checksum="6986F64E776B15B1" modified="2011-06-28" version="1">MLFYMIIAEGIRKKYGNKSALNGVSLKAEKGKITAILGPNGAGKTTLIRIILGLIFPDDGEVRILDEDPFKDKSIFKKIGYIQELPNLPPFLTGREVLEFSAKIKGVDKSEIPKLLEMVGMTENADKKIAKYSKGMIQRIAIAEAMLGNPEVLIMDEPNMGTDPVLITNFRNMVKNIAKDGRTVLMTSHEMEDVKKIADKVYFIFKGSVYFEGTVEDLIKRFLGIRVIVETEDIENLKAEANKINFIKGVEEEGKNKVILTLTEDKREELIKRLVLAGVRVRSFYLDNELEEAYLNIVKEAMSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B442</accession>
    <name>F4B442_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thiosulphate-quinone oxidoreductase (TQO) DoxD subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774923.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B442"/>
    <dbReference type="STRING" id="933801.Ahos_0113"/>
    <dbReference type="GeneID" id="10599553"/>
    <dbReference type="KEGG" id="aho:Ahos_0113"/>
    <dbReference type="eggNOG" id="arCOG02862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544122_0_0_2"/>
    <dbReference type="OrthoDB" id="199518at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007301">
        <property type="entry name" value="DoxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053663">
        <property type="entry name" value="TQO_large_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041179">
        <property type="entry name" value="TQO_large_DoxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04173">
        <property type="entry name" value="DoxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="TQO small subunit DoxD" evidence="2">
        <location>
            <begin position="20"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="184" mass="20410" checksum="28FA991107E0F6B5" modified="2011-06-28" version="1">MSGKQSEEFKRTEKMTRMEYLFPVRFAVGWMFLDGGLRKAVLKPAKLDPNSASFVGGKLVNFLPHAGPFKGLLLMTLENRSLDVTFLTVFSYIEIIAGLFIIIGLLTRLAALGALAMSVGFAPAYWLGSTCEDEWQIGALLTAGSVTLMLTAAGRVWGLDYFLYKKLGDRPIANVPILKWIKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B443</accession>
    <name>F4B443_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0114</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774924.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B443"/>
    <dbReference type="SMR" id="F4B443"/>
    <dbReference type="STRING" id="933801.Ahos_0114"/>
    <dbReference type="GeneID" id="10599554"/>
    <dbReference type="KEGG" id="aho:Ahos_0114"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="8"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="401" mass="43782" checksum="3BE72A5C0028A831" modified="2011-06-28" version="1">MSERPVSNYIGSYISWIMDSYDLGAVVITSTLLEKLFYPTLGTLGAVLPIVFTVLFRPLGGFIFGYIADKYGRKRTLIFTVLGYSLSIGITSILPTYYQIGILAPILLSLLRILQGIFIGGDVSSSFTTAMESVRKMRGLFGGIMQSGTLVGFVIVDLLFAYFASIPSLVLNSWRYIFAIGVLPAGLAVFIRSRMTEPKIYVESKKDNPIKGLKPIWQTILVMIGFWMMIYAGPQFAPVFFGEYLHLTQAEIGKYAFYMNLAGIPAMIIAGGISDFLGRKFSGIGYVALATIGAGIFYFSGFSLFTVMLFGFLINFPSALTPAYLAERFKTFSRATGVGFSYNGAFIAAGFSPLLISLVSTKLPLNTSAFSILTLGSIIAILGLALGPETLKNNELTMYQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D2</accession>
    <name>F4B4D2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type-5 uracil-DNA glycosylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0128</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D2"/>
    <dbReference type="STRING" id="933801.Ahos_0128"/>
    <dbReference type="KEGG" id="aho:Ahos_0128"/>
    <dbReference type="eggNOG" id="arCOG00905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083279_0_0_2"/>
    <dbReference type="OrthoDB" id="8612at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033958">
        <property type="term" value="F:DNA-deoxyinosine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10031">
        <property type="entry name" value="UDG-F5_TTUDGB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044147">
        <property type="entry name" value="UdgB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF3">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="10">
        <location>
            <begin position="49"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="222" mass="25333" checksum="E1D2ECFE8C4DED44" modified="2011-06-28" version="1">MANKYVQNMFDDEIISCYRCPRLRQYSEMIAKIKVKRFRNWEYWGKPLPGYGDENASLLIVGLAPAAHGGNRTGRVFTGDESGKWVTKALYELGLSNLEFSLSREDNLILRGVYLTNAVKCAPPENKPLREEILNCNYYLRKEITSLRNLKVILALGKIAFDSVCIAFNEKCKFSHGVVYDVKGKKIVGSYHPSAQNTKTGRLTWESFMQVVRKAYELTKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F3</accession>
    <name>F4B4F3_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774959.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4F3"/>
    <dbReference type="STRING" id="933801.Ahos_0149"/>
    <dbReference type="GeneID" id="10599589"/>
    <dbReference type="KEGG" id="aho:Ahos_0149"/>
    <dbReference type="eggNOG" id="arCOG07324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG10055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_468989_0_0_2"/>
    <dbReference type="OrthoDB" id="43795at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="509"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="581" mass="67595" checksum="3BD703705E92418C" modified="2011-06-28" version="1">MALIKLNLFLFFLLAFLFVTIIPTYHSLAVGEFEIFNETVIYYLPHYCIQKIILQEQIVKIFNNNTMLINQTVFLPNISAYMPWTTFIQPINCSDGFFYISPTKLGHNTSSFIFNGTEDGFYVYYNISYAFSSKYIFILYYNSSGIPVKGCDIKYCCGKIGMKAIYVLWKTNLITNESLPTFPGYTQARVINYNFSSLIPTEQASHYILLGIISLGISIIILILIFRPQVKMNDGGKKNFYNTPLFPLLLYTLEKIIVFIVFYLIFGNLFISKMVTNWDAIDYLWIAQYGYINKFLYAFSPLFPFLIRGLTFLTSSYPLSSFILTNIFGYIFVLLVYKYYGFKGSLVLSTFPVFVLYSTIPYSDDIALTFLILSLNLEGWLSSLSLAGAIATFYNLAYTLPSFLLKYRRKIWYFIPPLVVGGIILIAFWIFTGSPLTFFSAEDKYWYAYFTTPWCQAEWILNFYDYFINTFFAPCFHLSPLIYLLRNYAFLAFYITGTILFYKKYKNAFLTLYSSSIILPLFFIHGWPSISLPRLLLPSFPSLLGYVDIIFKRNAYTLIYIIISIFLTFFFTEWQVLAFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G4</accession>
    <name>F4B4G4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC-type sugar transport system, permease, UgpA, UgpE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0160</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. MalFG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774970.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G4"/>
    <dbReference type="STRING" id="933801.Ahos_0160"/>
    <dbReference type="GeneID" id="10599600"/>
    <dbReference type="KEGG" id="aho:Ahos_0160"/>
    <dbReference type="eggNOG" id="arCOG00158">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_496634_0_0_2"/>
    <dbReference type="OrthoDB" id="45815at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050901">
        <property type="entry name" value="BP-dep_ABC_trans_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243">
        <property type="entry name" value="MALTOSE TRANSPORT SYSTEM PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32243:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN TRANSPORT SYSTEM PERMEASE PROTEIN MALG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="46"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="338"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="548" mass="61298" checksum="1C7C558C5DD426C7" modified="2011-06-28" version="1">MKYSIPYLGYILGFGIIPFALTFAFVGLNAKTAFQGINLVPLNVIIYNTFFFSFFTALFSSIIGTFLAVGIDIISKGKRALSLLIMLPYTIPFTSSALIWTISLYGGYGWFTYFLGLKFDPLYMKCTALYAVTLVSIWSSIPMPFLIMLSALRSIPSYVKEAAEIDNLSLSEYYFRVALPMVGKAFWLSFLLEFILALGNFDLPYVLTSGGPGYATATLPLLVYEEMFSYDNFSGGSLAAAILSIIATIPSIALLFLLRSKRTGWVSLKIRMPNKVFKGIIFAFLALMLFFLDFPVYWMFLVAFRNSSLDFHYPPILIPKCLTPSYFSSASIQAIPYLVSSAVVAITASIFTIFIALPSAYEVSKGKLKFILPLSIYLYSLPSTSFVIPLYDFFSSEGLLNTWWALIVSTPIFTATFAVWLFFNFFLSFPKSYEDAAEVFSIRRKMTRIIMPLSRPALFSVFLLSFIFNWHLLFYPLIFTSTPYNYSFPPQGAQTITIFALLAIGDESINWGLLASSALVAALPVMVVTLFAIDRILKGSYSGGLKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I3</accession>
    <name>F4B4I3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4-methyl-o-phthalate/phthalate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I3"/>
    <dbReference type="STRING" id="933801.Ahos_0180"/>
    <dbReference type="KEGG" id="aho:Ahos_0180"/>
    <dbReference type="eggNOG" id="arCOG02691">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="311" mass="34278" checksum="B90BD35A22A1765D" modified="2011-06-28" version="1">MEWTPFKWRGLISGIIQGGFSFGAALAAVVEAAFIGFFGVKALDAYAWRYLFLTSLVPAVIALIIRITMKETPVFEDVRANKLIRKSPFLDLFRKPYRRDFFQVMLYMTGLFFSAYALFAFVPAILEHSPSIYGLGTADTIYSYGTYAAFAGAVTIGALSQIIGRRKITIIWAIITAIISIPIYYLLIYSATIGSVALSTLAVIIIGFTTQAPWGIVPIYLSERFKASMRASGVGFGYSSGIFIGGWFSIYVPLMHQYLFKSLDTPTNVWFSTAVLLILGALLVGLGVYLGPETLGTRLTEEVEESKVIPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J2</accession>
    <name>F4B4J2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutamate-1-semialdehyde aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0189</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J2"/>
    <dbReference type="STRING" id="933801.Ahos_0189"/>
    <dbReference type="KEGG" id="aho:Ahos_0189"/>
    <dbReference type="eggNOG" id="arCOG00918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="369" mass="42051" checksum="7D2A8C54DB16E392" modified="2011-06-28" version="1">MMHSHLKVFYEESFPVLKVTHKLPILKRGRGNRVWDIEGKEYIDFDMSNGDALLGYGNPELVNAILEGDSDQKETIEKLKRRFSLKSVKIFSSEKQAKVYAVNLARQLSGKKKIIRFGQNDLIKKCDLGEEIIAEWNNLDYLEKIVRQNYDVAAIIFEPIATHMGLVLPENDFLKRIMELSKEYGIITIADEIKTGGKYYTGASSYLKIKPDIILFGRSLAGSIPIGIVGMNYKEGDYVRASPISIKALGITLDELNEQSMYEMIRLNDILIKGYKDLIEDNKIKASVTGWGISGTIYFKESPPRKYSEFLEINVKKWNKYFKGMLDSGIIPMSNYDSQWSISKAHTEDDINVHLEKLNSVIKTTVLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J7</accession>
    <name>F4B4J7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775003.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J7"/>
    <dbReference type="STRING" id="933801.Ahos_0194"/>
    <dbReference type="GeneID" id="10599634"/>
    <dbReference type="KEGG" id="aho:Ahos_0194"/>
    <dbReference type="eggNOG" id="arCOG01205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159724_1_0_2"/>
    <dbReference type="OrthoDB" id="40412at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF3">
        <property type="entry name" value="POLYMERASE NUCLEOTIDYL TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14812" checksum="C97D32F0DF438C12" modified="2011-06-28" version="1">MKSFLDVYVEFVKEKMNLLANFCKIAPIIYEAIKEALKEYNLQGEIYFLGSVINGTYTAASDIDVAILLNEIPKERKEIEGKVLDYVINKGLPYWIPIEFHFLTPHSFKILKKGGANFVKAEDYILNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L6</accession>
    <name>F4B4L6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartokinase</fullName>
            <ecNumber evidence="7">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1363</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L6"/>
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    <dbReference type="GeneID" id="10600850"/>
    <dbReference type="KEGG" id="aho:Ahos_1363"/>
    <dbReference type="eggNOG" id="arCOG00861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_6_1_2"/>
    <dbReference type="OrthoDB" id="8904at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aspartokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042199">
        <property type="entry name" value="AsparK_Bifunc_asparK/hSer_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF70">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="312"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="445" mass="49431" checksum="83033E185DA73A0E" modified="2011-06-28" version="1">MIIIKIGGSIQKDERDYELISEKIEKYSNNEDKVLVITSAMKGITNDLISATENRDKSTEIIGNIYDKHIKILSKVADGPEFEIAFKDLSKMADELFKIAWSIRVLDEITPRVRDYILSFGERMATIVLNAALRSRRLDSIAYPEPPLVTDNNFGEANVLEDLTLKEINEKILSKKSKILIIPGFIGKTIDEKYTTIGRGGSDYTATLLGKLLNVENVRLVTEVPGIMTADPRKIESATTIKRLSLEEAVELAQMGAKRLHPRTFEPMFSSNMKVTIEGLYEEGETIVSGSCEDEDKLKGVTILDDLKMINIESTRIVGKIGSAARVMAEAKNSNVNIVSISQPASETTISLVVNSNDAEILATKLKEIKDIDNIEIKDVSAVSIVGCGLRNNEIFKEVENVALQYDILSMSRGLKNVSATFIVKKEEGYNLAKSLHEVVLKWIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M3</accession>
    <name>F4B4M3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Replication initiator protein WhiP, Cdt1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776168.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M3"/>
    <dbReference type="STRING" id="933801.Ahos_1370"/>
    <dbReference type="GeneID" id="10600857"/>
    <dbReference type="KEGG" id="aho:Ahos_1370"/>
    <dbReference type="eggNOG" id="arCOG04268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109465_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="236" mass="27645" checksum="3DC8C55AA7B21D95" modified="2011-06-28" version="1">MTDKDIENLAEELTKQLEGSPRSKLVEAIIILLHARPLRTSEIASNLGYQTKYISSYLSYWKKKGLVYQDGGRWYLTPQGENLAKAIIDSYSNSRFKEMLVIAKQMLEQVKEPMNNKNEQKVEKEKKAVLSFIDSKTNNIVKKQQNKDPTVCIKEILEKLDDDEKDILLYLINKYKQWGSTYVYIDQIQEEYKADPGWLFKVLRQLQTKRIVYLYQDPKLGLRIGFSKSFKEKLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N0</accession>
    <name>F4B4N0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776175.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N0"/>
    <dbReference type="STRING" id="933801.Ahos_1377"/>
    <dbReference type="GeneID" id="10600864"/>
    <dbReference type="KEGG" id="aho:Ahos_1377"/>
    <dbReference type="eggNOG" id="arCOG06043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161775_0_0_2"/>
    <dbReference type="OrthoDB" id="45678at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="126" mass="14847" checksum="4A4008E5C5CBF8D1" modified="2011-06-28" version="1">MQKRDICPFYKNGYCISPMLDSPSDIVISASRCFKTYKTCRYYVDQGEEKQGLENFQQYEKIDQDIKFYPKISLIQEKIDSGCEFFQLMKTENGFIASCKILNRIITESQAKLCSKYWQTCPLRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P1</accession>
    <name>F4B4P1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">flpA</name>
        <name evidence="10" type="ordered locus">Ahos_1388</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA. These sRNP particles form homodimers, giving rise to an asymmetric holoenzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776186.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P1"/>
    <dbReference type="STRING" id="933801.Ahos_1388"/>
    <dbReference type="GeneID" id="10600877"/>
    <dbReference type="KEGG" id="aho:Ahos_1388"/>
    <dbReference type="eggNOG" id="arCOG00078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_2"/>
    <dbReference type="OrthoDB" id="6244at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
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    </dbReference>
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        <property type="term" value="P:tRNA processing"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
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        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
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        <property type="entry name" value="PRK04266.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
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    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="109"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="134"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="233" mass="26311" checksum="C7D7AF652603C870" modified="2011-06-28" version="1">MSESIKSVKETKMENVFECEYEDGTTRLCTKNLAPGYSVYGERLIKYNGIEYREWNAFRSKLAGAILKGLKENPVKKGVKVLYLGAASGTTPSHVSDIVEKEGKVYGVEFSPRVVRELILVAQRRPNLFPILADARFPQSYMPLIENVDVLYVDIAQPDQTDIAIYNAKIFLKEGGSLLLAIKARSIDVTKNPEEIFKEEASKLERNNFDVKQVINLDPYDKDHAMVLARFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P9</accession>
    <name>F4B4P9_ACIHW</name>
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        <recommendedName>
            <fullName evidence="4">DUF373 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P9"/>
    <dbReference type="STRING" id="933801.Ahos_1396"/>
    <dbReference type="KEGG" id="aho:Ahos_1396"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048986_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF373 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39766" checksum="07295E5DE805EF00" modified="2011-06-28" version="1">MVKMIRKMAKTVIIYIDIDDDLSLAGIKSPVIGETNVKEAIDKASEIMADDSDFNSMIVAYNIYKKMKKEGQDVEIVFLAGSQKGGLEAQRRISAQLDEIIKELNPQDSIIVYDSPEDAKALPIIQSRLKISGVQRVIVEQHRGVEETYILLGKYFKKILNEPRYSRIFLGVPGAILFAAGILEVLGLISYIVPVITILIGSAMIIRGFDLDEMMEKWWENSTIMVIAALLSSISFAISLINGYFTYISIKGNSVYVVSTVISSMLPYITFSVLILLGAKALSSALDKSIKLFHDIIKISAIIISYYIITDVLKNLEEGIYIIQFQSFYALTISSMVLIFAYIVLNLIEKYKFSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R9</accession>
    <name>F4B4R9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R9"/>
    <dbReference type="STRING" id="933801.Ahos_1416"/>
    <dbReference type="KEGG" id="aho:Ahos_1416"/>
    <dbReference type="eggNOG" id="arCOG04135">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196483_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="97" mass="11187" checksum="E81B5960E580CF58" modified="2011-06-28" version="1">MKATFTVIFKNLNSAIKVMEEKYVCLTCGKTFYSGQGIILTVADRKLYFHSKACAYKFLKEVILNVDKDCISSTVKDVYKKYEDLAKKREENAKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U8</accession>
    <name>F4B4U8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775030.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4U8"/>
    <dbReference type="GeneID" id="10599661"/>
    <dbReference type="KEGG" id="aho:Ahos_0221"/>
    <dbReference type="eggNOG" id="arCOG00503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036012_0_0_2"/>
    <dbReference type="OrthoDB" id="7773at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052926">
        <property type="entry name" value="Metallo-beta-lactamase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754:SF18">
        <property type="entry name" value="7,8-DIHYDROPTERIN-6-METHYL-4-(BETA-D-RIBOFURANOSYL)-AMINOBENZENE-5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="278" mass="30488" checksum="9CB8CF509B83ECBB" modified="2011-06-28" version="1">MKRLSNLKMTILSDNFVSTLIPPLIGEWGFSALIETDDTKILYDVGNSGYPVLYNAEKLGVDLSKIDYIVLSHGHSDHTGGFSNPELLKKLEGKIVIAHPSVFEKKLLNWSGKIEYIGIPMSLDEMEKHFHVILTSQPLEITEGVIFSGEVKRYGYDEYTAGLFTARDSSITGDHMKDDAALYMNTEKGLVVLTGCGHSGILNIINHAKEVTGENVYAAIGGFHLLSSPKDKVVKVSEELLKLDKVGPAHCSGNLIKSLIADHKDKFIDAGVGKVIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Y6</accession>
    <name>F4B4Y6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">2-keto-3-deoxy-(6-phospho)-gluconate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0260</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DapA family. KDPG aldolase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775068.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Y6"/>
    <dbReference type="STRING" id="933801.Ahos_0260"/>
    <dbReference type="GeneID" id="10599700"/>
    <dbReference type="KEGG" id="aho:Ahos_0260"/>
    <dbReference type="eggNOG" id="arCOG04172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_2"/>
    <dbReference type="OrthoDB" id="350860at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053415">
        <property type="entry name" value="ED_pathway_aldolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040954">
        <property type="entry name" value="Arch_KDGaldase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="283" mass="31944" checksum="77D9FA02C7C00B81" modified="2011-06-28" version="1">MTEIIVPILTPFNEKGEIDGEKVKTHVQNLLRKGVDIIFVNGTTGVGPALSKDEKRKMLDYVLDVTNKVIFQVGSLNMKEVLELVNYANERDIIAVASYPPYYFSIPKDFVVKYFKEICEASKHPVYLYNYPGATGKDVDVNLAKEIGCIKGVKDTNPDFAHTIRYKELGIKTYNGLENLAIASLSILDGTVVGAGNYMPELFSQLREYIKAGDMRKALDLQLKINEIDGTTIPYGKISSMYYLVKELQGYDVGVPRPPMYPLEEDKVKELIEKVRKIWNKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B531</accession>
    <name>F4B531_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776248.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B531"/>
    <dbReference type="STRING" id="933801.Ahos_1450"/>
    <dbReference type="GeneID" id="10600946"/>
    <dbReference type="KEGG" id="aho:Ahos_1450"/>
    <dbReference type="eggNOG" id="arCOG00506">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066633_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07709">
        <property type="entry name" value="flavodiiron_proteins_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041">
        <property type="entry name" value="HYDROLASE, METALLO-BETA-LACTAMASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="256" mass="29438" checksum="B8D8E4680E060E8A" modified="2011-06-28" version="1">MIEPIVLAEEKGNKFVWLSIDEAEMDKGIITNQYLLTVKDKGVLMDPGGPLVFERVYNAMQKFIKPEDVEYIFLSHEDPDIVSGLSLWLSYCPNAKILISDLWDRFLPHVGVDIKMNVIRIPIQGMQINLNGDDLRIIPAHFLHSVGNFHLYDPITKVYFSGDLGAAVFPKGSWYIIVDNFDEHKKYMEDFHKKYMANKKAIELWLKHIQGLDIRIIAPQHGAVIEGDNVRKFIDWIKSLDKIGIDLLEEELNTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B572</accession>
    <name>F4B572_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphomevalonate kinase</fullName>
            <ecNumber evidence="2">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1491</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776289.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B572"/>
    <dbReference type="STRING" id="933801.Ahos_1491"/>
    <dbReference type="GeneID" id="10600991"/>
    <dbReference type="KEGG" id="aho:Ahos_1491"/>
    <dbReference type="eggNOG" id="arCOG01032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076270_0_0_2"/>
    <dbReference type="OrthoDB" id="36273at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053661">
        <property type="entry name" value="GHMP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001174">
        <property type="entry name" value="HddA/FKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040957">
        <property type="entry name" value="Arch_PMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00960">
        <property type="entry name" value="LMBPPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="7">
        <location>
            <begin position="57"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="8">
        <location>
            <begin position="228"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="316" mass="35255" checksum="6AB93106AD78481E" modified="2011-06-28" version="1">MISAPGKVLWIGSYAVVFGGLSHVIAINKRVKCEATPSDKLIFETTYGTFYEKGNELIESVVNVIKNHLGDIPRLKIRLFNDEDFQINGKKTGLGSSSAATVTLTACLYKQIKGRIDTDEIHLLAQEANFIRQKGIGSGFDIAAAVYGSIIYKRFTDLNKRDWKIERLRLGNKYDMILGFTGRSSETVNLVKMFIEKKDDEKFIEFLREINIENDTAIKLLKLGKVDEASQHAKLARDLLNVVAEDVIGVKLQSEEDKKLIKIAEDNGAYISLLPGAGGGDVIFALGENLDEVRKKWKEKGLKLIEIKEDDGLNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B573</accession>
    <name>F4B573_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776290.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B573"/>
    <dbReference type="STRING" id="933801.Ahos_1492"/>
    <dbReference type="GeneID" id="10600992"/>
    <dbReference type="KEGG" id="aho:Ahos_1492"/>
    <dbReference type="eggNOG" id="arCOG05974">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381430_0_0_2"/>
    <dbReference type="OrthoDB" id="34438at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="22719" checksum="722BEB0D3BBFBE65" modified="2011-06-28" version="1">MDFLDKKKRLVLIAIAQKGTSGLTYDELLSTFLIFMTRNTLQSIIEDLYFSGYINILRDDKEVRLIASRKVRSSLVALDFQRYKLLKFLEEVKKKSEEISKLQDKNSQLEEIKKIGKEGLELISSGLLTLYKEFPEFTIPEYIEIVEVINNEVLAKLSPLITQQMTEEDLKQFLNLTSKYLGEKEAEVLSEALKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B589</accession>
    <name>F4B589_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thermosome</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0292</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775099.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B589"/>
    <dbReference type="STRING" id="933801.Ahos_0292"/>
    <dbReference type="GeneID" id="10599732"/>
    <dbReference type="KEGG" id="aho:Ahos_0292"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="547" mass="59351" checksum="F2A68844FD3A6944" modified="2011-06-28" version="1">MLPRENLASIFRENTSRESGKDVILSNITAVRTLAEMLKTSLGPRGLDKMMVSSTNDITITNDGATIVKDLEVQHPAARIVVETAKVQDSEVGDGTTTAVVLSGFLLDEAGKLLDQNIHPTTIIEGFKKALDKALAISKEIAIKINPEDRNYLREVAYTTLSSKFFSEGEELDKIIDLSIDAVLSVMDKVGDEYVIDLSNIKMVKKRGESVEDTELIKGFVLDKEVAHEGMPRRIDEAKIAVIDFPLEVQKTEISSKLSLTSPEQIKAALDEQAKYLRGIVDKIAATGANVVICQKGMDDIAQYFLAKKKIMGIKNVSRSDLEKIAKTTGATIISSEMDLDSSALGYAKVVEERTVGKDKAIFIEGVKNAKVVTILIRGATDIAMDEVERSLNDVLNSVRNVIIYPYVVAGGGAFEEELAMRLRKETLPGKEQLALEAYANALEEIAVTLAETAGLDPTEALVNLRTMHAKGLDKSGVDVMQGKLVEDMTKIKVVDSLKVKEQVLKGATEAATAILKIDDLIAAAPAKQQPQQQEGGYPGMSGMPGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B592</accession>
    <name>F4B592_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        </citation>
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        </source>
    </evidence>
    <sequence length="405" mass="44700" checksum="1D9ED55C159FB239" modified="2011-06-28" version="1">MGKDLNKGSISLIGLTFITIAGVLPIIAPIEVAAFISDAGGAAIWPVILGYLLFLAVSLPILEYTRLVSFSGGYYGLAEIGFGKTVGKFTALCNYLFYNSWQMANAFFIGWLIVDTLYILYGIMLPCYDWIIISILTLFITFLITIQHPRNLSRILIIAILSSLILVIASALYVILKSPYNSLYYLNPSSSPSGFTGIALATAVVGFYTFTGYASPLFYSEEGIESRKNVWKAIYLGLTISAITIALVAYSEVISVPSSNLQVIGSSSMPQLVSWINYLPPIFLLMLNIIIAIISLIAFGGGSGAQARLLWAMSRDKFIKSEWINRLNKNNVPRNAALLNLIFALSIVLIASLLMMHYYGYNPNTVETAFYVAGTLSTILWYFHHFIPEMGYSLSCKNTRNLNFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E0</accession>
    <name>F4B5E0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF871 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0343</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775150.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5E0"/>
    <dbReference type="STRING" id="933801.Ahos_0343"/>
    <dbReference type="GeneID" id="10599784"/>
    <dbReference type="KEGG" id="aho:Ahos_0343"/>
    <dbReference type="eggNOG" id="arCOG06045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065324_1_0_2"/>
    <dbReference type="OrthoDB" id="25415at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008589">
        <property type="entry name" value="MupG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043894">
        <property type="entry name" value="MupG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043797">
        <property type="entry name" value="MupG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38435">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38435:SF2">
        <property type="entry name" value="DUF871 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05913">
        <property type="entry name" value="MupG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19200">
        <property type="entry name" value="MupG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phospho-N-acetylmuramidase N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="6-phospho-N-acetylmuramidase C-terminal" evidence="1">
        <location>
            <begin position="235"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39533" checksum="5DFCC3526DE9769A" modified="2011-06-28" version="1">MKSVGILSQAWRREKINELKEIAEKAGKMGYNEIWSGVDPNYIEGIKEISAVAEKYNMYFFIDINPDIMRGFGASPTNLKVFKDLKIGGIRLDYGFSIEDTIKMANNDLGLKIELNASIFPLDKLDYVVKNVKDIENLKASHDFYPIKYTGLSLESAIAKSKEFKERGIPVAVFVAPPREIEGRNTIETLRGKSIGKAASILFNSRYIDRVILGHPFPTDEELKELIEAKEYTTIRVLVYPGLTEEEKKVFTVFSDVRVKEYTIGLSKMMENNIKPRNIVRRFRGAVTVMNCRKNVVEVWIFKKEVEADSRFNVIGEVYYDDMDLLDYIGENMNVKLVPIYMKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I1</accession>
    <name>F4B5I1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776320.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I1"/>
    <dbReference type="STRING" id="933801.Ahos_1522"/>
    <dbReference type="GeneID" id="10601022"/>
    <dbReference type="KEGG" id="aho:Ahos_1522"/>
    <dbReference type="eggNOG" id="arCOG08449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998622_0_0_2"/>
    <dbReference type="OrthoDB" id="376430at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="124" mass="14441" checksum="0CCBD1E271605270" modified="2011-06-28" version="1">MNQISPKGFSQKLVKEIKMNDGWKYSLLEIKVNESHYVHIIGNSEFSTGVSVDEKGNVIRIYANRIKVLNETEDIKEEEIEQTIVELKSKKIIHMKSKLNLNKKEQGFGRIKSMEIINQEEYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K4</accession>
    <name>F4B5K4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776343.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K4"/>
    <dbReference type="STRING" id="933801.Ahos_1545"/>
    <dbReference type="GeneID" id="10601045"/>
    <dbReference type="KEGG" id="aho:Ahos_1545"/>
    <dbReference type="HOGENOM" id="CLU_656573_0_0_2"/>
    <dbReference type="OrthoDB" id="372509at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="4"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="418" mass="48110" checksum="B5216EBE2BE489D1" modified="2011-06-28" version="1">MKKIIINIEGSVNSGKSTLLGYLLWKYSTNKDYIAQKTRLFQIYNKQYKVPVQEYLANLVQITSDETIRQMTSKSHAVNITIGDLQLIAIDIGGHNRYTYQAHLSTVESDTTLFAIPVDEIKEKAYLKNTMLTHLITTYLVRKAFGRTPEFHVIITKIDMEKDYDKYVSQVKSDLDRIGKILGMTDARFYYYTSSIIVKGINDIQAEGQYEELLKNLESTVQTRINSSKTVFHARDVMNPTENIKVLAGRVMSGKIQKNDVLNTPYGEQKVIQIEEFGQKIYAADPASYIAIRFERDNFPLNFKGGEVLGNFEYFSSGEITLLDLRDTVKRIQEEFELKDEQYYNPPSKIQKGTKFYTTINGYRTALKVEDVISEESKKLRLKVKIEDRVDKISVFGEIRNTGITYEENNKIWGAFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M9</accession>
    <name>F4B5M9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Electron transfer flavoprotein alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1570</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776368.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M9"/>
    <dbReference type="STRING" id="933801.Ahos_1570"/>
    <dbReference type="GeneID" id="10601071"/>
    <dbReference type="KEGG" id="aho:Ahos_1570"/>
    <dbReference type="eggNOG" id="arCOG00447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_2"/>
    <dbReference type="OrthoDB" id="307696at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000004">
        <property type="entry name" value="Electron transfer flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF11">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN, SUBUNIT ALPHA (ETFA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="5">
        <location>
            <begin position="50"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Electron transfer flavoprotein alpha subunit C-terminal" evidence="4">
        <location>
            <begin position="159"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="282" mass="30740" checksum="88AE5C38F3AA99B9" modified="2011-06-28" version="1">MKVVIYSEDPEIIKVGASYFPNEHIIGITTREVKYTDELYLVDMIDEEFISNFIASLKPDVVLVGNSKRDKTVAGYVAGLLKVPIIPEVMDLEGNKAKRVVYSGMGIAEVEFSYPAVIIIGRKEVQVKEKEKIEINKLEVKEGKVKAIEEKSKGEQGVNLSTAEIIVSVGRGIGSKENVKYAEDLANALGAALGGSRPVTAELGWLPEDRQIGLSGNKVKPKLYIALGISGQPQHLAGIKDAKIIVAVNKDKNAPIVENADYTIVGDAIEFCKIMTEKVKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N4</accession>
    <name>F4B5N4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="4 14">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_0358</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="14">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14">Mg(2+) or Mn(2+) required for ssDNA cleavage activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="4 14"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775165.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N4"/>
    <dbReference type="STRING" id="933801.Ahos_0358"/>
    <dbReference type="GeneID" id="10599799"/>
    <dbReference type="KEGG" id="aho:Ahos_0358"/>
    <dbReference type="eggNOG" id="arCOG00786">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125304_0_0_2"/>
    <dbReference type="OrthoDB" id="26676at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF6">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF83" evidence="15">
        <location>
            <begin position="3"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE93249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="184" mass="21452" checksum="D4B528E316546C5E" modified="2011-06-28" version="1">MISGTTVKHFAYCPQIVRLEAMGFTERVTEAMREGLEVDKEKTINSLYGVLKPLNIVAKPIFRYNDLVGSPDYVLFFSNYVTPLDVKQGKERIDHVMQILFYLYIMEMKGFNVKEGLLYYVQQGRLKKVRYSFRERNHVTRIIQEIRNAMKGKVKVTQPVSKCKNCGFFHWCRPKIEGSIAVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P6</accession>
    <name>F4B5P6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P6"/>
    <dbReference type="STRING" id="933801.Ahos_0370"/>
    <dbReference type="KEGG" id="aho:Ahos_0370"/>
    <dbReference type="HOGENOM" id="CLU_2490235_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="86" mass="10263" checksum="8C139A1DFA9E6CAA" modified="2011-06-28" version="1">MRDEEVDKFLEELLRQYLERSKVTNDSFTDMVNNALLKIRILDKLSKKNINIINANGLYRDLFNFLNRADKDKLKSTFAYVYIKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P7</accession>
    <name>F4B5P7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P7"/>
    <dbReference type="KEGG" id="aho:Ahos_0371"/>
    <dbReference type="HOGENOM" id="CLU_2339984_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="97" mass="11155" checksum="791F5AD0EFE0652E" modified="2011-06-28" version="1">MEFIITCSNGLTYSTVYLYIKNNKDVIRRYIPQDSVQNVPVNLIDFVRNNSFLAHGGFILPSTPDVIENIYGRLLDDTSLKLNDFLDKINKIKLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q6</accession>
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    <organism evidence="3 4">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F4B5Q6"/>
    <dbReference type="STRING" id="933801.Ahos_0380"/>
    <dbReference type="KEGG" id="aho:Ahos_0380"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
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    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
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    </dbReference>
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        <property type="entry name" value="AAA_14"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="138"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="35342" checksum="3279F217AA7BEFC4" modified="2011-06-28" version="1">MKKRNNERAYLFLDEVTSIDEWWRIVKYFIDSGEFKNVVITVSGSSTLRLTKIPEKFPGRRGKGKDVQVMPLSFPEFAQIKSGKTKEELLFDTSLTLSLFEDYLKVGGFPKSINNNPDSSQALIQGVLSEVYKAKMSAEIVQNLIYSILDKIPSAMSFNSIASDLGISHNTVSEYIEFLKDIFVVGIAYLKRGEEIIKRKEKKVFFRDPFILRSMSLWVNKEFDESALLENVVQEHLLRKEGEVAYYKNSEEIDVITKNYKIEVKMKRSHKSYPEDIMILDKDEIPFFLIKSLDLKLGNTDSSQRR</sequence>
</entry>
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    <accession>F4B5Z3</accession>
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        <submittedName>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Z3"/>
    <dbReference type="KEGG" id="aho:Ahos_1613"/>
    <dbReference type="eggNOG" id="arCOG12780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168594_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="242" mass="28067" checksum="99FF471179CD9D6C" modified="2011-06-28" version="1">MGLNLILSYIDLDVDDVYVSSLLKIMIEDKRLLREHFELLLSRLSSLTPRQQLRVLDYILYISNKNEMGLTSLKIQCSPREVVYSITLLSLNPRIVSLYEALKICDKKLEILNKFTNNLEDIILNPKLIDFLREKINGEGFDKAHLELLILLEFALKLRNEGYHVKLNDFYGDLLLDTNASFEEQMKIRDQIIEEYKRKYNVDVSAEVGDVFGMVDSVDKIKEYMRKILDVGGGRKQCLLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Z8</accession>
    <name>F4B5Z8_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1618</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776411.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Z8"/>
    <dbReference type="GeneID" id="10601118"/>
    <dbReference type="KEGG" id="aho:Ahos_1618"/>
    <dbReference type="eggNOG" id="arCOG07990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179007_0_0_2"/>
    <dbReference type="OrthoDB" id="194703at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="90" mass="10633" checksum="5004413823773FB5" modified="2011-06-28" version="1">MATTIKDKDKTKLDLESLRAEILLKSNIKLTMEELQDYLISFAIDNKESFFDYVMKKFKRKNYEKMKEAIKDWGIDTSNIDVDSVIYDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B604</accession>
    <name>F4B604_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="AlphaFoldDB" id="F4B604"/>
    <dbReference type="GeneID" id="10601124"/>
    <dbReference type="KEGG" id="aho:Ahos_1624"/>
    <dbReference type="eggNOG" id="arCOG03712">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="OrthoDB" id="41609at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="169" mass="19241" checksum="A83E9A88C37AC402" modified="2011-06-28" version="1">MGEGIVPQIKDLQNYVNFKLEEVIVELNLALELLQRGYTKNASQKAFIAWKAIVSVLVSINLEKMPKNEKEREWYNKVGFLAPTTSLNGIARRLEEIGYKDIIGLNSLALKLHRYAYNGLYRGASDYVDKNDAIEDLKVLIRKITELISSLGKSEDSKKILNKLENINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B615</accession>
    <name>F4B615_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1635</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_013776428.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B615"/>
    <dbReference type="STRING" id="933801.Ahos_1635"/>
    <dbReference type="GeneID" id="10601135"/>
    <dbReference type="KEGG" id="aho:Ahos_1635"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_4_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07721">
        <property type="entry name" value="yflN-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF17">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="231" mass="26359" checksum="8F86283CBF0380AB" modified="2011-06-28" version="1">MLLTKGVRIIELFEPEFFGTVLNHNVTIVEKGPSGGLMMIDTGLPGYLDIIEKGLKSFGYSLEDISDVVITHYHIDHSGNAEEIKKISKAKIYAHENEVPYLSKNEQKFNLKYDDVKDELKVSEEDFERTMKRINSMKFSPVTVEVKLKGEEEIGNFKVINVPGHTPGHIALYDGNLLIVGDAVRNIKGKISPPFKFFCWDYEKAVNSFNNLLSMKFKYLVPFHGDIIFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B638</accession>
    <name>F4B638_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger SWIM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775242.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B638"/>
    <dbReference type="STRING" id="933801.Ahos_0438"/>
    <dbReference type="GeneID" id="89920731"/>
    <dbReference type="KEGG" id="aho:Ahos_0438"/>
    <dbReference type="eggNOG" id="arCOG09871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392713_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="11178" checksum="F0BC29E92B039829" modified="2011-06-28" version="1">MYSYAITERHEKTKDVYIFVVDSEENPFESYIVKIEVGREKIRASCSCKGFALRGYCKHLELSLKKLRIYRGLYLQRWERRSDSDLMDNEEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B659</accession>
    <name>F4B659_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0459</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">The Mn(2+) ion enhances activity.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775263.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B659"/>
    <dbReference type="STRING" id="933801.Ahos_0459"/>
    <dbReference type="MEROPS" id="M20.008"/>
    <dbReference type="GeneID" id="10599899"/>
    <dbReference type="KEGG" id="aho:Ahos_0459"/>
    <dbReference type="eggNOG" id="arCOG01108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.360:FF:000001">
        <property type="entry name" value="N-acetyldiaminopimelate deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053493">
        <property type="entry name" value="Thermostable_CP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040868">
        <property type="entry name" value="carboxypep_CpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF63">
        <property type="entry name" value="METALLOPEPTIDASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G09600)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="3">
        <location>
            <begin position="188"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005962-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="396" mass="43607" checksum="6685383BDA12A64F" modified="2011-06-28" version="1">MNIEEIAEKLLKDSESIENDVITIRRILHENPELPFQETNTSRLIEEKLRSLGIQTRRLSTTVIGLIDSGKPGKTVALRVQISALPITEKTNLQFSSKSSGIMHACGHDANVAMLLGAAQLLVKNKDLLSGKVKLIFQAGEEEDLGAKEVISNHELDDVDYVFGLHVSPFIPSGFFATRKGALMPSSSNFKIRVKGLGGHVSSPHSTLDPIFISAQIVNLLDGLTSRIVNPLDGFTLSITSIHSGTKSNIIPDEAVMEGTIRGFDVFTIEKVKSKIKSLVDSLCKSFNADCEVVFSDNCPPLINYPEITSRAMDILNNLRRPIVEIEPVMLADDFSRYLQLKPGCYIFIGTRNLEKGCIYPTHSPMFKLDEDILKYGSAALALLAISFSKEESIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B666</accession>
    <name>F4B666_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved conjugative plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B666"/>
    <dbReference type="KEGG" id="aho:Ahos_0466"/>
    <dbReference type="eggNOG" id="arCOG05320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117041_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009705">
        <property type="entry name" value="DUF1286"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06939">
        <property type="entry name" value="DUF1286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="189" mass="21680" checksum="D382414F2F5A16EA" modified="2011-06-28" version="1">MLLGKVQILIRIIIVMKLRTHYVFSLGLLSLLDSFLLSNFLEVIIISGIISVTANNVIDYLGHEIRGKYISRTPRTHTLPRSIGWGLLTSVPIVLALYFLSSTELILITILDGVIVGPSHMLLDIFTERGIYHKVNGRWRRIALAHFSYDNPIVNGLAIIAGVIMLFAAIHNHNYDYYYQYYHYYNYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B677</accession>
    <name>F4B677_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Citramalate synthase</fullName>
            <ecNumber evidence="7">2.3.3.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0477</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.21" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B677"/>
    <dbReference type="STRING" id="933801.Ahos_0477"/>
    <dbReference type="KEGG" id="aho:Ahos_0477"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_7_0_2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07941">
        <property type="entry name" value="DRE_TIM_LeuA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005675">
        <property type="entry name" value="Citramal_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00977">
        <property type="entry name" value="citramal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43538:SF1">
        <property type="entry name" value="(R)-CITRAMALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43538">
        <property type="entry name" value="ALPHA-IPM SYNTHASE/HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR00977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="511" mass="56513" checksum="82EB84913107E5D4" modified="2011-06-28" version="1">MDTTLRDGAQTTSVSFTLNDKIRIALALDDLGVHYIEGGWPGSNPKDEEFFKEISKYSLKAKVAAFGSTKKKGVRAEEDKNLNAIIDSGVKVAVLFGKSWTLHVTHVLKVSKEENLELVYDSIRYLRDHGLEVIFDAEHFYQGYKEDEEYALEVIKTAEEAGASVIALADTNGGTLPHEIYEITSRVVKELKAKVGLHMHNDSGCAVANTLMGVLAGARHVQGTINGLGERTGNADLIQVIPNLQLKMGFKVIPDLTKLKGVSRLVYELSGLHPNPYQPFVGDNAFSHKAGVHADAVMKVPRAYEHIDPTLVGNQRRIVISELAGSSNLLNYLEKIGIKVDKKDERLRKALQKIKEMENKGYSFDLAPASAVLQILKELGIYTKFIDLQYWKVINESGNLSLAVVKVNSQLQASEGVGPVHALDMAVRNALQKVYPELSRVKLIDYRVVLPGEIKNTESLVRVTIEFTDGKDNWRTEGVSTSVIEASVIALIDGLDYYLQVSKKLKTEILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B679</accession>
    <name>F4B679_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775283.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B679"/>
    <dbReference type="STRING" id="933801.Ahos_0479"/>
    <dbReference type="GeneID" id="10599919"/>
    <dbReference type="KEGG" id="aho:Ahos_0479"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_8_2"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01143">
        <property type="entry name" value="YvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="20"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="30834" checksum="E4F26507771AD27D" modified="2011-06-28" version="1">MKIYNQVLDDYVEIRRPLKKIVSLDPATTETLFFLGLGDKIIATDAFSYRPEEARKLPKIGSYTHVKLDFLEENKPDLIFSTMGAQKDLTKKLINMGFTVYPMQVATSVSKIIDNVIIIAEVVDRKNEGRELVNTLRKKIAENYNPGNIKVYVEFDLGGPITMGFPSHVSDAISIVGGKNIFDDKDEAYFIPDDKEILNRNPDLFVYEPKRLTDYEKERFLKKMEERGLQQFRDRLVFTKGDYLAHMGPSFILDSIPWLRSLLTPLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B686</accession>
    <name>F4B686_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775290.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B686"/>
    <dbReference type="STRING" id="933801.Ahos_0486"/>
    <dbReference type="GeneID" id="10599926"/>
    <dbReference type="KEGG" id="aho:Ahos_0486"/>
    <dbReference type="eggNOG" id="arCOG00284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453183_0_0_2"/>
    <dbReference type="OrthoDB" id="10575at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="187"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="603" mass="68822" checksum="72A54330AB4FF6AB" modified="2011-06-28" version="1">MTSDELINNIKDRVDKAKALAITLGSIIGKVSPNSPNKVDEENYTVNVVVDPYTYYKYSFLGRIGVFLGAIDIKTLYFILLRVIGYERSDVKSFLFDNNFVSNVEEETPGTLINNVTLKCEMLTKIDILSSSEPSPADIVIEPQSPVILPNADLIERSLSLNRGLLKVGILDYLDLDVKVSLSLDDLNYHALIIGTTGAGKTSFIKDLISGLYTIGEEGTKVFILDATGDYYHIFLPPDFTDKGVKQGIAEFEKLYGKLNKLETDVIFPITKSWIRKYAKSGKIESITSAYHELYVKPLLDYLDMKGLKVYSSVIGNQIILSNSQWNSKINLHPFFFRYRDVRKILYKLNPYFSEQASHFLKIFSSSKEAKECKTLEELIDLLDDSEFDKFKLHKSTRENILRGLYLLRETELFDLRAQRDLLVNVVKNSGNLIIFDIYNGELDDLSQKILTYYFLDRLFAFRERQMREGNIKGRFLIIIDEAHRFFPSSKGGEEDTNYVKRVAGKIATMMRLGRRRKIGFIFSTHNPSDLSDIIVQLANTKFIFRISKEIAEQLGLTPTEAKMLSWERNGVAYLISPWLRQGKVKIKVPVPPPIGHYDLSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B687</accession>
    <name>F4B687_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NurA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B687"/>
    <dbReference type="STRING" id="933801.Ahos_0487"/>
    <dbReference type="KEGG" id="aho:Ahos_0487"/>
    <dbReference type="eggNOG" id="arCOG00287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_755697_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="59"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="369" mass="41456" checksum="B57C4192C4640A56" modified="2011-06-28" version="1">MQSQQNIMEILNSIVVNYVSKYLTITSLDDYVELNSTTSPSVLENEDIFFEVHPSSFTQEIYAVDGSSRSFVSGKGIISVSAVAISSSSIPIYGVYPSLDGEKELELKEPFIAVASSVSENSKIDPYLYFGPYVSPISITGDPFYSTEGFEVIESEIRSVLETKALNVVKGKGKIIVDGPLFPSYLYLTSKFKEKILEERKKIIDSNFIGIVKRLDKSRGLIDSIDDRTRSIIYQKFRIDPKIFLSDESFILHLVRFNYSPPYKILALGPFSKRICNNITVYMYYLIIPFHPYVSKFSILRIETLTKDPTILGMIASMGITKDGIPPLLALADFKAKKTSLALYRYIVSIVERLGIQASFYSRLSGELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D7</accession>
    <name>F4B6D7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776472.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D7"/>
    <dbReference type="STRING" id="933801.Ahos_1680"/>
    <dbReference type="GeneID" id="10601180"/>
    <dbReference type="KEGG" id="aho:Ahos_1680"/>
    <dbReference type="eggNOG" id="arCOG00662">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054041_0_0_2"/>
    <dbReference type="OrthoDB" id="15118at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01098">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="26"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="347" mass="39386" checksum="3D9111CEB81942A9" modified="2011-06-28" version="1">MISSPDWVRLSFGADMVLGFSPGKFLKGALNTTINLLEYYPDGCKANCIYCGQAREVAKGPECKTLIRVEWPLRKLDDVLNRIKEREGNPEYGLQRICVGQLAHPRASPDAIEITRRIREKGIELQISELVTATYTFKQHMIEMRKAGADMIDVAIDAASKRVFDQVRGKPVRSMHRWERYIQAIDEAVEVFGKKNAGIHLIVGLGETEKEAVELIWYAHSRGAKISLFAFYPELGTPMQNKKPVPLNVYRRIQLARWLIENDLVKLEDFTFDEEGKIVDINIPQDLSLKELAPAFMTSGCPGCNRPYSNERPGSVLKNYPWYPDEKQTLSAIKASKIPSLIKRFVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F0</accession>
    <name>F4B6F0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1693</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F0"/>
    <dbReference type="STRING" id="933801.Ahos_1693"/>
    <dbReference type="KEGG" id="aho:Ahos_1693"/>
    <dbReference type="eggNOG" id="arCOG05916">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102440_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="179" mass="20794" checksum="BD92538E905F2B2C" modified="2011-06-28" version="1">MLDVDINNTVSGNAKKSLQTILDAMELKSKTLRSLEISTEEDARKLFESVFYARKHFSDVISKTSIPELSYAFSQLKDPSLSYSERVEKFKSTVKGGEREDIEDMAKEIIHFLEPEKYPLWTRWIWNKERGTGSINYVIKDDVKINDEKELFSTIDELRNVLEIFGLESKGKYYITSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F6</accession>
    <name>F4B6F6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1699</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776491.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F6"/>
    <dbReference type="STRING" id="933801.Ahos_1699"/>
    <dbReference type="GeneID" id="10601199"/>
    <dbReference type="KEGG" id="aho:Ahos_1699"/>
    <dbReference type="eggNOG" id="arCOG02066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139144_1_1_2"/>
    <dbReference type="OrthoDB" id="33055at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF2">
        <property type="entry name" value="PEROXIREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14505" checksum="4AA40C686FF1D21D" modified="2011-06-28" version="1">MSQEQEQKKKILIVVTHGPEDLDRTYAPLFMASIAASMEYETSVFFMIKGPLLLSKAWQEEERKKGNNPFIHFFDMARDNGVKMYVCIQSLKDMCHMNESDVVDGVELVGGSTLIDLTMDADRTLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I9</accession>
    <name>F4B6I9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776524.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6I9"/>
    <dbReference type="STRING" id="933801.Ahos_1732"/>
    <dbReference type="GeneID" id="10601232"/>
    <dbReference type="KEGG" id="aho:Ahos_1732"/>
    <dbReference type="eggNOG" id="arCOG02441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_527494_0_0_2"/>
    <dbReference type="OrthoDB" id="86287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="51706" checksum="F27A36CC9FFAADA0" modified="2011-06-28" version="1">MRPFIYDFKKTFLRLTSISLLFLFILSGVGIAFETFHLTHITPASYENLNVEGYVCPSGNKISVYGIAFNNNGKPISSALVCVLSSGNVVASTRTSANGFFNLTVSYSPCLSLLVKYNGLERNVNISRIVGELQNNVNSQVVSVTIYPNIFSNTSCFYSLNACWCRILVSIMCQDNLVIFSSKNVNISMELVHSSGIIGNKSVVLKQINLYLKKFTPVYATIEIPQGVNKIVLEPINSSPNIQQIGLNDFEYIKGTAIYCDTMNNFLTLLNIFMLFLPYTIIYLAYISFSRPRSIGEMEFILARPITKRKIFISRFFSNALIAVLSSILIVLAPAITFNAIAGLHIPLQVIFLLVLIPMSLLLTYLSVTYMYSSIIKSSGTVQGLGMFTLLFLEVILPFIGIFTSTYETLKQYITPASISYYIYSLIEGCNVRINVGIEITSTLLWIIIPAIISYLEFKIRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J0</accession>
    <name>F4B6J0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1733</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776525.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J0"/>
    <dbReference type="STRING" id="933801.Ahos_1733"/>
    <dbReference type="GeneID" id="10601233"/>
    <dbReference type="KEGG" id="aho:Ahos_1733"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="299" mass="33317" checksum="402720079EDB2590" modified="2011-06-28" version="1">MIAVERISKRFGSKKVLDNVSFDVKDGEIAGFVGLNGAGKTTTIRILAGVLTPNSGDVLIDDLSIVKEKEKASMHVGWVPELPLFDPNAKALDYFVFIAGYYGISKSDALSLGKRLFEEVGLAGREKDKLRNYSQGMKRRFLLAVSLISDPKNFLFDEVLNGLDPEGIQFFRDMALKFKKEGKSVLFSSHILSEVESIADKVIFIHQGKILRIMEMNEIRKIVKSNDLKIILGKVDDNVLSIAKSFGKPELNGNTLIIHDFNGELSELSSKLSPYEVLEVSRLKNSLEDVFFKIINGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L2</accession>
    <name>F4B6L2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L2"/>
    <dbReference type="STRING" id="933801.Ahos_0534"/>
    <dbReference type="KEGG" id="aho:Ahos_0534"/>
    <dbReference type="eggNOG" id="arCOG06048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093138_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="231" mass="26702" checksum="9032D1242566C2E6" modified="2011-06-28" version="1">MLRLIGGAAVAVIAPKGSELFSRTYKDADYFGLSSQRKEITSLLEGLNMEPNKRFNALHGYNRLMFYDPVLNSTIDVFLDEFVMCHKLILKDRLKIYYPSIPPSDLLLTKMQIINLTENDKKDIAALLYDFEIGDKDEDKTLDGNYIAKLLSEDWGFYKTFTINHDRMKEYLKGNTYAPNILPKLDKLREMIESHPKSLKWKMRAKIGERVKWYEEPEEVNTNFQGAGSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M0</accession>
    <name>F4B6M0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biotin--acetyl-CoA-carboxylase ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775346.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M0"/>
    <dbReference type="STRING" id="933801.Ahos_0542"/>
    <dbReference type="GeneID" id="10599984"/>
    <dbReference type="KEGG" id="aho:Ahos_0542"/>
    <dbReference type="eggNOG" id="arCOG01940">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_3_0_2"/>
    <dbReference type="OrthoDB" id="46252at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="230" mass="26094" checksum="6C488B04058FAAF1" modified="2011-06-28" version="1">MLKFKLSKVTSTQDFAEAISSMLYEDFLVIAEEQTKARGRYKRAWYSPKGGLWFTYVKKNFNAEEIPVATLKASLAVRETLSSFFDAKIRWPNDVVVNDKKVSGILIEAIYAGDATDLFIGVGINTNVKEFPPDIKATSILLETGKEVNNDELLNNVISKMDYYLTLKEDFEEIVGKINNYLSIKDKKVVITRKDETKVECTALFVDKFGRLVTDCGIFEVEDVLRVESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U1</accession>
    <name>F4B6U1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTPase subunit of restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776549.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U1"/>
    <dbReference type="STRING" id="933801.Ahos_1757"/>
    <dbReference type="REBASE" id="35547">
        <property type="entry name" value="AhoW1McrBP"/>
    </dbReference>
    <dbReference type="GeneID" id="10601257"/>
    <dbReference type="KEGG" id="aho:Ahos_1757"/>
    <dbReference type="eggNOG" id="arCOG03779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_465911_0_0_2"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="278"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="564" mass="65514" checksum="B776BB7A39065F83" modified="2011-06-28" version="1">MYGEWLTVRPQFAKLFDDSSEDEFISYIKADKFIPCIFVGDKNNWKASIEYSIKGELGYILWGSYDETSYKGIWDRYNYAYIIKNISSNNNLLPNANKILLTLFWMSKGNNKNENYGVIGIGILSDINIDVTRAFKGWSETTSRYWIVRYRAKVIWLHRKVRESIKTSQGLEIDNNLWDGDKIGNISVRNNICYKDENSIKNAREEIKKFIDRIKDEIPETYKFYKNFDSSRNITSQVIEEQSKEILCNRRQKMDLSNFFLPGNNASNLGLLLTKAIEKSNIMFIGPPGVGKTKLAIELARSLSGNNECLNIVTANSLWFRRNLIGGESIREGSVIWKSGLLIQAYVKASRIKEGNYYVIIDEINRADVDKAFGELFTIFSSGDPKDWFIPKALIDEIKSYGDNIDQYAKEFLKIYEELQKQGKENEPLKKIRIISTMNLTDARNLFYVGDALARRFVGFYFDYPKSTEDLDMFLKNYNLGREEKEEIRNLVSYLREKLDKDKLVKFNISPASLKSALEIYSSLENRNIDTFILILKSTLGTLNQDKIEKVEKLIDEWRKEKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U6</accession>
    <name>F4B6U6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">site-specific DNA-methyltransferase (cytosine-N(4)-specific)</fullName>
            <ecNumber evidence="2">2.1.1.113</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1762</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776554.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U6"/>
    <dbReference type="STRING" id="933801.Ahos_1762"/>
    <dbReference type="REBASE" id="35546">
        <property type="entry name" value="M.AhoW1ORF1762P"/>
    </dbReference>
    <dbReference type="GeneID" id="10601262"/>
    <dbReference type="KEGG" id="aho:Ahos_1762"/>
    <dbReference type="eggNOG" id="arCOG00890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027633_1_0_2"/>
    <dbReference type="OrthoDB" id="38200at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase N-4/N-6" evidence="9">
        <location>
            <begin position="34"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="403" mass="47805" checksum="8921545353596F8C" modified="2011-06-28" version="1">MQTSIDDFLNNKYRPVLFRDIVKLRTNYATHDLYPYPAKFIPNVVRYFIEAYTKPGETLFDPFAGSGTVAIEAEITGRNYILWDLNPIIEILVKAETWKDEISEKIFEIDFNYNKSFIPKWKNLGYWYPKEFLEQLSKLWAYYHDHPNPLVAIPLFKITKYFSYAELEFPKLYKSKFAIERVNGLLNSNWKEIMRDMYWKEVERVIRKVKEFQSLCKCVSSGKVYGGVDVLSESPPNVDAVITSPPYLQAQEYIRTFKIELYWLGYDDNKIRELSKKEIPYNKPPNVEIRSKTFKEYLEKIEEFGNKKLVDIYITYFRSLAYFFGKVNARTLGIFVGPVKVRRLRVPIDEILKEHLESLGWKHEVTFIDKIVSRRIKEVNKNPATKLEDERTPTEHLLIMRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B733</accession>
    <name>F4B733_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Elongation factor 1-beta</fullName>
            <shortName evidence="6">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ef1b</name>
        <name evidence="8" type="ordered locus">Ahos_0628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775432.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B733"/>
    <dbReference type="STRING" id="933801.Ahos_0628"/>
    <dbReference type="GeneID" id="10600075"/>
    <dbReference type="KEGG" id="aho:Ahos_0628"/>
    <dbReference type="eggNOG" id="arCOG01988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165896_1_0_2"/>
    <dbReference type="OrthoDB" id="84643at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001670">
        <property type="entry name" value="PRK00435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="7">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="91" mass="10005" checksum="33AE1F7998391BA6" modified="2011-06-28" version="1">MTDVLVVLKVFPDSDEVNLDKLAEEISKKLPQNYSIVRKETEPIAFGLQALVLYVKMPEETEGGTDTLEEIASNVEGVSHAEVVGITRLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B6</accession>
    <name>F4B7B6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3800 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776651.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7B6"/>
    <dbReference type="GeneID" id="10601362"/>
    <dbReference type="KEGG" id="aho:Ahos_1863"/>
    <dbReference type="eggNOG" id="arCOG11298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1471997_0_0_2"/>
    <dbReference type="OrthoDB" id="377262at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="21327" checksum="85668576D8437308" modified="2011-06-28" version="1">MNKYHVFVDESYDLAVFVLAIVWVSSINEIKRIKSDALKKLGKPKYNFHFIDDPPTIRNTFLGEILNSALNMGVLLSSRQPKSCMEYAKFYVTEVVRVLPIKNSLLFLHVKGLKNWVSKRCDILAEAVKLGIENGNYVKLDLRANEREGIEIADYVASSFRKCLHEKEDYCNALERKLKFISYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C0</accession>
    <name>F4B7C0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776655.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C0"/>
    <dbReference type="GeneID" id="10601366"/>
    <dbReference type="KEGG" id="aho:Ahos_1867"/>
    <dbReference type="eggNOG" id="arCOG07211">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387510_0_0_2"/>
    <dbReference type="OrthoDB" id="379324at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="23345" checksum="CDA0EAA00AEA5D6F" modified="2011-06-28" version="1">MKKWKLVSITLFIIIVSYLLLHYISYLHYISYLLCNMRFVTITSTKYKQNPTIGNYAFIISSGIASENILNKNLITSYVYINLSKVKPGDNFLIYPPINNGSKISRAFYSNPIINVTILNSTIFYSHGDQYINFGIKVYITAYKKIAQGCVISIIGMPLESFSSTELSCGTNLYIGFKAVNASVFELMFFVANCATHTTSKSLFSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I4</accession>
    <name>F4B7I4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA methyltransferase complex GCD14 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0702</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775505.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7I4"/>
    <dbReference type="STRING" id="933801.Ahos_0702"/>
    <dbReference type="GeneID" id="10600154"/>
    <dbReference type="KEGG" id="aho:Ahos_0702"/>
    <dbReference type="eggNOG" id="arCOG00978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_0_1_2"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF1">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14801">
        <property type="entry name" value="TrmI-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="6">
        <location>
            <begin position="62"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="263" mass="29668" checksum="B9B29EF8F59D89E8" modified="2011-06-28" version="1">MRFIHVPIQEGDPVTIWIDNKRAFLIKVKKGKRLDTDKGYIIHDQLIGKEYGEIIKIAKGEAYLLKPTPMDIYTTLPRPSQVVYPKDASYIIYASGVQPGDTVVEAGTGSGFLTITLAYFLGPNGKVISYDVREDMQNKAKQNALMLNLLDRIEFKIKDIRQGIDEKDVSAVILDMPDPWNAVSKAYEALKPSGSLIVFVPTVNQIEKTVLQMRNEGFIDIHAEELILREYQVKENAVRPKNIGVMHTGYLIRGRKYIKGTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L5</accession>
    <name>F4B7L5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl14e</name>
        <name evidence="5" type="ordered locus">Ahos_0733</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775536.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L5"/>
    <dbReference type="STRING" id="933801.Ahos_0733"/>
    <dbReference type="GeneID" id="10600187"/>
    <dbReference type="KEGG" id="aho:Ahos_0733"/>
    <dbReference type="eggNOG" id="arCOG04167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183474_0_0_2"/>
    <dbReference type="OrthoDB" id="63594at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000045">
        <property type="entry name" value="50S ribosomal protein L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00721">
        <property type="entry name" value="Ribosomal_eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023651">
        <property type="entry name" value="Ribosomal_eL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003320">
        <property type="entry name" value="PRK04333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="4">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="10701" checksum="DB7A2536993F5E6E" modified="2011-06-28" version="1">MPAIEVGRICVKTRGKDAGKKCVIVDIIDNNFVLVTGPKKITGVKRKRSNILHLEPTDKKMDIQKGASDDEVEKKLQEAGLTDFMKEKVKIKIPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T2</accession>
    <name>F4B7T2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776744.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T2"/>
    <dbReference type="STRING" id="933801.Ahos_1956"/>
    <dbReference type="GeneID" id="10601455"/>
    <dbReference type="KEGG" id="aho:Ahos_1956"/>
    <dbReference type="eggNOG" id="arCOG12785">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131548_0_0_2"/>
    <dbReference type="OrthoDB" id="39537at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="245" mass="27512" checksum="680C635907A58E26" modified="2011-06-28" version="1">MITLKLLNSQITYDGEVILIKENIPNSETSVEIFADGISKLSLETPHYRITGKNLYLPSKEIDLADVKSFAYSAIRNIIREDKEPIIALSGEGLVYDFSVNCHEVKIIEKTLNGIHFLSAALQKPLLPSSVSVQLPPWATPFKLSSAVILLTEKPQDVLKKHFQQITKIVTDSGYIEKNGLRLVRLYYGGFNKVLCYSQLSGYLCFEEEEKVKETRPSDKDIEKLANNILKGYGKCVIEKGMIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W6</accession>
    <name>F4B7W6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="11">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="11">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gatB</name>
        <name evidence="13" type="ordered locus">Ahos_0757</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 11">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W6"/>
    <dbReference type="STRING" id="933801.Ahos_0757"/>
    <dbReference type="KEGG" id="aho:Ahos_0757"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="486" mass="55580" checksum="23CE4D30E471FE7D" modified="2011-06-28" version="1">MIYYRMHLGYRVIKMPKIGLEVHVHLNSLKTKLFCSCPTDYVGKEPNTVVCPVCLGLPGAIPVLNEEALKKAIMVAIALNCEIAPYLIFSRKHYFYPDMAKNYQISQYDGPGSMAIAKAGYLNIGNKKVRIRRINIEEDPAKSVYPSGSMLTSKYTLLDYNRSGIGLLEIVTEPDIEQPKEAREFLNKLRSILEHLDVCNCEIEGSMRADVNISVEGGERVEVKNVGSIRDVEDAIKYEVARQKAAIAQGLKVTRETRHWDAERKVTVPIRLKESEEDYRYFPDPDLPPYQITEDYIRKVKESMPELPDARMKRIVEMYGITEYDAEVLVSDKALADLFERTAKLYNNYKKLVNVIINDYLRWINDKNLRISDSKATPEALAELMNMMDKGIISIKILKEVLPQIILEGKNPREIIEKEKLNVISDKDYLEKIIDEVLEEDKDAALAAKKDPKVINYLVGKVMKKTRGKADPYLTNQLLKEKLQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B803</accession>
    <name>F4B803_ACIHW</name>
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        <name evidence="1" type="ordered locus">Ahos_0794</name>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="GeneID" id="10600255"/>
    <dbReference type="KEGG" id="aho:Ahos_0794"/>
    <dbReference type="eggNOG" id="arCOG07188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168021_0_0_2"/>
    <dbReference type="OrthoDB" id="43379at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="121" mass="13926" checksum="44A0CED9999BB884" modified="2011-06-28" version="1">MIEISQTTWSADKKNIIRFDGKCVIQVIKDIPPKSVINQACYIFFKTYTKSRIKEPEIYFLTLLYSTSQIANALAHVKFKEGDIVYVIKCCEKDINNFEQIQIKDNSERIALSTNAINSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B898</accession>
    <name>F4B898_ACIHW</name>
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        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776837.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B898"/>
    <dbReference type="STRING" id="933801.Ahos_2051"/>
    <dbReference type="GeneID" id="10601551"/>
    <dbReference type="KEGG" id="aho:Ahos_2051"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_9_1_2"/>
    <dbReference type="OrthoDB" id="42541at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF79">
        <property type="entry name" value="MOBA-LIKE NTP TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="139"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="267" mass="30068" checksum="B7311E50B1AF2541" modified="2011-06-28" version="1">MRTSGHVDLTLAAFFWGTIGIAVNYFYLLGGSPFTMVIMRSIFSTIVSLFIVKKIVVNKYTIGMGFVSSLFYLTYIYTIPIDGPSLSAVLLYTAPLWVSIFSYFLVKEKATLGKIISSLMILIGLYIMYLGVPTLQQFLLGLASGLTYAGLISFSRLLQRKGMSDWEIIFSQSLWSIPFSLPFVRVLNLPSVYAGIYLAIFATVIPYFFFYRGMKKTDSITASIITALEPVFTIILSMIIFREFLNIYETIGTIIILFSIFLSVKMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D5</accession>
    <name>F4B8D5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Small ribosomal subunit protein uS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps19p</name>
        <name evidence="10" type="ordered locus">Ahos_0848</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D5"/>
    <dbReference type="STRING" id="933801.Ahos_0848"/>
    <dbReference type="KEGG" id="aho:Ahos_0848"/>
    <dbReference type="eggNOG" id="arCOG04099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="141" mass="16431" checksum="BF561601C76566B4" modified="2011-06-28" version="1">MAMSEFPAEWQKFKYRGKSLDELINMPMDEFVKLLPSRQRRSLTRGFTPQQRRLLEKIRKMRREGKLTKTIKTHVRDLVILPEMVGLKFGVYNGKEFVEFQVVPEMIGHYLGEFAITTKKVEHGEPGLKATRSSLFLAMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F5</accession>
    <name>F4B8F5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Adenylate kinase</fullName>
            <shortName evidence="12">AK</shortName>
            <ecNumber evidence="4 12">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">adkA</name>
        <name evidence="13" type="ordered locus">Ahos_0868</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="4 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775670.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F5"/>
    <dbReference type="STRING" id="933801.Ahos_0868"/>
    <dbReference type="GeneID" id="10600336"/>
    <dbReference type="KEGG" id="aho:Ahos_0868"/>
    <dbReference type="eggNOG" id="arCOG01039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="OrthoDB" id="26198at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003122">
        <property type="entry name" value="PRK04040.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="8"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="195" mass="21491" checksum="ED331000D0F542E1" modified="2011-06-28" version="1">MKMGIVTGIPGVGKTTVLSKVSEILSLENRKYKIMNYGDYMLNTAISEGFVSNRDEMRKLPLSKQRELQLIAAKKIVEDAEKLGEDGLAFIDTHAVIRTPAGYLPGLPKHVIEILSPQVIFLLESSPEDILNRQKRDKGRIRSDYADINVINETINFARYAAMASAVLVGASVKIVINKEGDPSIAAKEIISSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I8</accession>
    <name>F4B8I8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776855.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I8"/>
    <dbReference type="STRING" id="933801.Ahos_2069"/>
    <dbReference type="GeneID" id="10601569"/>
    <dbReference type="KEGG" id="aho:Ahos_2069"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF13">
        <property type="entry name" value="UDP-D-APIOSE_UDP-D-XYLOSE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="301" mass="34032" checksum="21A730F776E043CA" modified="2011-06-28" version="1">MTSVVTGGAGYIGGHLVDHLIENGEDVISIDDYSSGRYVNEKAQNIKLDLRIAYPEIPKEAIIYHLAANPDVKTSMENIYEHFERDVKVTLNVMEMARKYDAKMVIFFSSSTVYGEGKIPTTELDEIKPISNYGLFKVLGEEILNFYARNYGIRGVSLRLANITGGRISHGVVIDFIKKLRKNKEVLEILGNGKQRKSYLYITDLLSAVDVIVRYFKGLYDSFNIGNDDWVTVDEIARIVEEEMSLSPKHVYIDAGEGRGWEGDVRFMLLDISKIKSFGWLPKYSSAQAIRLAVRDVLDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I9</accession>
    <name>F4B8I9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I9"/>
    <dbReference type="STRING" id="933801.Ahos_2070"/>
    <dbReference type="KEGG" id="aho:Ahos_2070"/>
    <dbReference type="HOGENOM" id="CLU_2801991_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="67" mass="7538" checksum="86331EC6B391C954" modified="2011-06-28" version="1">MGIGLILLSALVIYVSFSIFFLGLKIKLGGLDISPIIIKIINFVIIFLVFIYLAYVGYIMIFHAQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J2</accession>
    <name>F4B8J2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_2073</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J2"/>
    <dbReference type="STRING" id="933801.Ahos_2073"/>
    <dbReference type="KEGG" id="aho:Ahos_2073"/>
    <dbReference type="eggNOG" id="arCOG00188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_749282_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF27">
        <property type="entry name" value="ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="197"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="340" mass="39033" checksum="9023080333467A49" modified="2011-06-28" version="1">MLKSVLCDSQNQVILDGKDFCKEKDYSKVSLVLQEPYSQILAETAKEEVELLSRYHRVNEEIVKNIMGEYYDKKFLELSDGYKRRFVISTTLASMPEYVLLDEPFANLDKYSTAEVKEIIPKGTLIAEHRVKEIRDMLDRVYLLEDKIEEIDKEKLYDENFLKSHGLRGFKLSKIESKLGKEILDVQTTKARIKVRESEVLCLIGKNGVGKTTTLKQLVGKVYVIFQNPDLQFFCTTVREEVKGREDVLSLFNLKDKEEKSPYTLSYGEKMRVLIASAYASGRKVIALDEPSVGMDGNSLLSFYEMIKLLKEEKRGIIIATHDEDIIGMCDSIIDLDKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J3</accession>
    <name>F4B8J3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Resolvase, Holliday junction-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_2074</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J3"/>
    <dbReference type="KEGG" id="aho:Ahos_2074"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881165_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651:SF1">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="14172" checksum="D7370640F848B04B" modified="2011-06-28" version="1">MHYGFNAVRIPTSNSSPNPLPDVFATKGNILLSIEVKSTWQKKVKVEENQIRKILDFLKMFPMEGRALIAVKFKGGIGWRVKEVELAKEEEVNEENSMPLENYLLNLQSNLLQSLNRRPYQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N5</accession>
    <name>F4B8N5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N5"/>
    <dbReference type="KEGG" id="aho:Ahos_2116"/>
    <dbReference type="HOGENOM" id="CLU_3112839_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="50" mass="5902" checksum="3F0C79D55EFDAC27" modified="2011-06-28" version="1">MLEMLNAEDGKEIEVIGDEDLLKKFKLLLLSLNQEIIVEEKNRIVYRKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N8</accession>
    <name>F4B8N8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Biotin carboxylase subunit of acetyl-CoA carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2119</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N8"/>
    <dbReference type="STRING" id="933801.Ahos_2119"/>
    <dbReference type="KEGG" id="aho:Ahos_2119"/>
    <dbReference type="eggNOG" id="arCOG01590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45007">
        <property type="entry name" value="CARBOXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G07570)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45007:SF1">
        <property type="entry name" value="CARBOXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G07570)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="7">
        <location>
            <begin position="6"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="125"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="513" mass="57528" checksum="B0B804DD83DF9757" modified="2011-06-28" version="1">MVKMPPFGKVLVANRGEIAVRVMKAIKEMGMKAVAVYSEADKYALHVKYADEAYYIGKAPALDSYLNIDHIIDAAEKAHADAVHPGYGFLSENADFAAAVEKAGMTFIGPSSDVMNKIKDKLDGKRVAKMAGVPIAPGSDGPVSSLDEALKIAEKIGYPIMVKAASGGGGVGITRVDNPDQLVEVWERNKRLAYQAFGKADLYIEKYAVNPRHIEFQLIGDKYGDYVVAWERECTIQRRNQKLIEEAPSPALKMEEREKMFEPIMKFGQIIHYFTMGTFETAFSDVTREFYFLELNKRLQVEHPTTELIFRIDLVKLQILLAAGEHLPFTQEELNKRVRGAAIEYRINAEDPLNNFTGSSGYITYYKEPTGPGVRVDSGVEAGSWVPPFYDSLISKLIVYGESRAYAIQAGIRALNDYKIGGVKTTIELYKWIMRDPDFQEGRFSTSYIAQKTEQFTKYLRQQEELRAALALEIQSRGLNRQGATPVTTSQRQPKSAWKTYGLVSQASSRVMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q4</accession>
    <name>F4B8Q4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">[LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase</fullName>
            <ecNumber evidence="7">2.6.1.118</ecNumber>
            <ecNumber evidence="7">2.6.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lysJ</name>
        <name evidence="9" type="ordered locus">Ahos_0888</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:41952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="2.6.1.118"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + 2-oxoglutarate = [amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:52672"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="2.6.1.124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="7">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. LysJ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.118" evidence="7"/>
    <dbReference type="EC" id="2.6.1.124" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775690.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q4"/>
    <dbReference type="STRING" id="933801.Ahos_0888"/>
    <dbReference type="GeneID" id="10600359"/>
    <dbReference type="KEGG" id="aho:Ahos_0888"/>
    <dbReference type="eggNOG" id="arCOG00914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_2"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00038"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02084">
        <property type="entry name" value="LysJ_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053458">
        <property type="entry name" value="Class-III_PLP-Dep_Atrans_LysJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037537">
        <property type="entry name" value="LysJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045491">
        <property type="entry name" value="LysJ_Sulfobales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="5 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="207"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="387" mass="42921" checksum="ED8FD474BFF2A2F4" modified="2011-06-28" version="1">MKLIEFYATRGLRIVKGENQYVWDDKGRRYLDLHAGHGVAFLGHRNKIVLEYLERQMSEIITLTTAFDTPIREEMLNEMEKIKPDDLDNVFLLNSGSEAVELALKTARKITGKRKLIAFKNSFHGRTSGSLSVTWNKRYREPFEPLLEPVEFLEFNSIEDLKKIDENTAAVIVEPVQGEGGVIPAKEDFMKALRETTQEKGVLLIVDEVQTGFGRTGKVWAYQHFGIKPDIMTAGKAIGGGFPVSVVFMPDWIAEKLSEGDHGTTYGGNPFAAAAVTGAIKALLADNVPEQARVKGEIFMKMLNEALADFKSVREVRGLGLMIGVDLRLNPGKAIKYLQDHGVLSLKAGVSTIRFLSPYMITQEDMELAVNATRQGVAITEGKTISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T0</accession>
    <name>F4B8T0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal-transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0914</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775716.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8T0"/>
    <dbReference type="STRING" id="933801.Ahos_0914"/>
    <dbReference type="GeneID" id="10600386"/>
    <dbReference type="KEGG" id="aho:Ahos_0914"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_2_1_2"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="63"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="124"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="239" mass="26632" checksum="017B0CCAF4DE2687" modified="2011-06-28" version="1">MLVKDVMSPDVIKVTKDTKIYDALNIMIRNNIRRLVVESNGIVTIRDIVYNWGKIDDTVDKIMTSDLQFIDKNSDLKEACRIVTAKGIGSLVVGNGDNIEGIITERDLIRYCKADINSFVQDIMNKNPLIISADTTLAEVVEFMKQKYVRHAIVACDNLPCGVISTKDIGKALLAKKDLTKTEVSGFMSNNVFKVYPDDKIETARMLMAEKNIGFLPVTNSKEILGSLSEREILAVLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B918</accession>
    <name>F4B918_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776956.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B918"/>
    <dbReference type="STRING" id="933801.Ahos_2170"/>
    <dbReference type="GeneID" id="10601673"/>
    <dbReference type="KEGG" id="aho:Ahos_2170"/>
    <dbReference type="eggNOG" id="arCOG05955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493010_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="19819" checksum="4023BE86291B7641" modified="2011-06-28" version="1">MVELSIESNYSNNVSSTEEIKVGHALAHIVAAASIVEEIEGELPEVNDTVRKYADDWIMEMAPIDYYPGLAEMLGCRIKKKLTQVFDEISEEELATTLDDVIELKRALDNGEIIYNYADIEVRVERLLRALGVEINDFASVLDTPDFYLKLRRLLAILVVAIGISSVRTKWIAESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B929</accession>
    <name>F4B929_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ribonuclease</fullName>
            <ecNumber evidence="12">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_2181</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 12">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the RNase HII family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B929"/>
    <dbReference type="STRING" id="933801.Ahos_2181"/>
    <dbReference type="KEGG" id="aho:Ahos_2181"/>
    <dbReference type="eggNOG" id="arCOG04121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="OrthoDB" id="33866at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07180">
        <property type="entry name" value="RNase_HII_archaea_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE95052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="192" mass="21876" checksum="D2997E903DFC5AE1" modified="2011-06-28" version="1">MVIAGVIIDEKIEGMLKEVGVKDSKILSREKREKLFEIISEFAEAYIVNKAYPNEIDSSNLNELTYSKVIQIIYASLPLHPKIVTIDKVGNEDIVIMKIRELGLKDNVVFNADVKYIEASAASIIAKVTRDRIIDSLKKIYGDFGSGYPSDPKTRKWIIEIYEKDKNNPPPIIRRTWKTLLKIAPNYYISKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B961</accession>
    <name>F4B961_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rhomboid family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0968</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B961"/>
    <dbReference type="STRING" id="933801.Ahos_0968"/>
    <dbReference type="KEGG" id="aho:Ahos_0968"/>
    <dbReference type="eggNOG" id="arCOG01768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529264_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF69">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="9">
        <location>
            <begin position="60"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="203" mass="22601" checksum="FFBD1E5ABA5A71CD" modified="2011-06-28" version="1">MGSHDSGIFSTNVFNWCIMRETLILAFLVVLGYVIGLLTSIYFPSIFIYLIQVNYLVLKGDYISLLTSIFVTNSLLDAGFNTIALLVIYSLFGSKAGKMEYIVFFFSGLLGNIFSLFLFPPNTASAGASGGIFGILSFYIIYDMLKDRKFDLYEFLILVIVFVLSDMLPNVDYFAHIGGILGGVILAFIASWLKRDGEGRYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B985</accession>
    <name>F4B985_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0237 protein Ahos_0992</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0237 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775794.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B985"/>
    <dbReference type="STRING" id="933801.Ahos_0992"/>
    <dbReference type="GeneID" id="10600465"/>
    <dbReference type="KEGG" id="aho:Ahos_0992"/>
    <dbReference type="eggNOG" id="arCOG04941">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155669_0_1_2"/>
    <dbReference type="OrthoDB" id="27277at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04872">
        <property type="entry name" value="ACT_1ZPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000032">
        <property type="entry name" value="UPF0237 protein SP_0238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01054">
        <property type="entry name" value="UPF0237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050990">
        <property type="entry name" value="UPF0237/GcvR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022986">
        <property type="entry name" value="UPF0237_ACT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001220">
        <property type="entry name" value="PRK00194.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34875">
        <property type="entry name" value="UPF0237 PROTEIN MJ1558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34875:SF6">
        <property type="entry name" value="UPF0237 PROTEIN MJ1558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13740">
        <property type="entry name" value="ACT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="90" mass="10042" checksum="26C66EAD9381BD83" modified="2011-06-28" version="1">MENAIVVVVGADKPGIVAGISSKLAEENVNIVDISQTVLRGIFAMIMLVDISKSKVPLSELRKELQQKGKELNVEVLVYHEEVFKYMERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B993</accession>
    <name>F4B993_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775802.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B993"/>
    <dbReference type="STRING" id="933801.Ahos_1000"/>
    <dbReference type="GeneID" id="10600473"/>
    <dbReference type="KEGG" id="aho:Ahos_1000"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_0_2"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="380" mass="42446" checksum="3E63F7BBD01B6ADD" modified="2011-06-28" version="1">MKRIVIAGGGIAGTIVANRLARQLSPELEKGEVEIIVINASDRHIYQPGQLLIPFGVTDTGEIIRNEKELLDSKIKLVIKEISKIDVQNHSVITADGTAYNYDELVIATGSHLQWDEVPGYRAVYSPWDLESAQKLREVLQSFTGGTVVINVAKLPHKCPVAPMEMTLMLDDYLRRRGLREKTEIIYTYPVPGVFGIKTTNDVMIKIFEERGIKVVSPFNVTRVNPEEKTIESQEGEKLKFDLALGVPPHKGAKVIEDSGIGDRRNWVPTDKFTLKMKDHSDVYVIGDTTDLPISKAGSTADFESYVVAGNIVNDIRGNNSTKLFDGSVFCYIATGLDSGTYIRFNYNYPPIPPPPSYVHWWGKLLYNKLYWTVTAKAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B995</accession>
    <name>F4B995_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1002</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775804.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B995"/>
    <dbReference type="STRING" id="933801.Ahos_1002"/>
    <dbReference type="GeneID" id="10600475"/>
    <dbReference type="KEGG" id="aho:Ahos_1002"/>
    <dbReference type="eggNOG" id="arCOG06224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131634_0_0_2"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01127">
        <property type="entry name" value="TrwB_TraG_TraD_VirD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="13"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="241" mass="27702" checksum="6E693F8DEAEDE234" modified="2011-06-28" version="1">MDLGFPLNSSELVRKCIAILGIRGSGKSNTAKILGKELIKEGFPLVVIDPDGEYDFQEALRIEDFNLDPEVVAKDVLERKRSLIIDMNEWNDEAFNFLFKFLDSFWNLSKIYRTNVFLVIEEAHEFIPQGERTKISEEITRLALRGRKRGIGMIMISQRSARVNKDVLTQSEVYFLHKVVHPVDLKVYREILPLKAKEVADVVKSLDVGEALFYMNGRVEKVKIRKYDESTTNSQFAENLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B999</accession>
    <name>F4B999_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775808.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B999"/>
    <dbReference type="STRING" id="933801.Ahos_1006"/>
    <dbReference type="GeneID" id="10600479"/>
    <dbReference type="KEGG" id="aho:Ahos_1006"/>
    <dbReference type="eggNOG" id="arCOG02692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="7"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="397" mass="43265" checksum="C813AC2AB9D6C337" modified="2011-06-28" version="1">MKSYIHSTISSSLAWAGNIYDLLLITYVYGILENMFNLNYFEISILFALGLIGRVLGGMVFGKYADTIGRKPVLMIGTGGYALFQGLMAFSPNVILLFIFRLIEGIFMGAEWTAGTVIAYEQAPRSLKGFVTGIVQAGYGIGYSLTGVTYLIFISSIANNWRIFLLTGALPLLLLPYIQLKVEDTKEQGSSKIAIRYKEYMGVLLKATLGMSGMFTAYFAVFGNYTEVAEVDHLPAYVLGILMTIANIVLAFSFILFGRLADRYDKKKLIYIGLIGLMIGLPFAVPLLSTMINAVGMSAGVIIFAFFTGFWPLMPLLLADAVPAEVRGFLSGFAYNAGGFVGGIADIIIGLLTLIYGINTISRWVDGFGFASILLVLVSVITWPRSGAKAKVIMIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E9</accession>
    <name>F4B9E9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(2Fe-2S)-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777010.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9E9"/>
    <dbReference type="STRING" id="933801.Ahos_2224"/>
    <dbReference type="GeneID" id="10601729"/>
    <dbReference type="KEGG" id="aho:Ahos_2224"/>
    <dbReference type="eggNOG" id="arCOG01925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_3_1_2"/>
    <dbReference type="OrthoDB" id="37184at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000003">
        <property type="entry name" value="Carbon monoxide dehydrogenase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000020">
        <property type="entry name" value="Xanthine dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051452">
        <property type="entry name" value="Diverse_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053590">
        <property type="entry name" value="Glyceraldehyde_DH_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041020">
        <property type="entry name" value="glyceraldDH_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44379">
        <property type="entry name" value="OXIDOREDUCTASE WITH IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44379:SF5">
        <property type="entry name" value="OXIDOREDUCTASE WITH IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="163" mass="17996" checksum="4E1E2D792D62D40A" modified="2011-06-28" version="1">MKVIEKDEKVKIRLKINGKDYETEVEPRKLLVHVLRDLGFTSVHVGCDTSNCGACTVVMNGKSVKSCTVLAIQADGSEILTVEGLSKDGKLHPIQEAFWDKHALQCGYCTPGMIMQSYWLLSENPSPSEDEIREGLAGNLCRCTGYQNIIEAVKEASQKLKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G6</accession>
    <name>F4B9G6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9 10">L-aspartate oxidase</fullName>
            <ecNumber evidence="4 9 10">1.4.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_2241</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the oxidation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-aspartate + O2 = iminosuccinate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25876"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="1.4.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="4 9 10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777027.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G6"/>
    <dbReference type="STRING" id="933801.Ahos_2241"/>
    <dbReference type="GeneID" id="10601747"/>
    <dbReference type="KEGG" id="aho:Ahos_2241"/>
    <dbReference type="eggNOG" id="arCOG00572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_3_2_2"/>
    <dbReference type="OrthoDB" id="23539at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00551">
        <property type="entry name" value="nadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="L-ASPARTATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716:SF2">
        <property type="entry name" value="L-ASPARTATE OXIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 10" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="11">
        <location>
            <begin position="4"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="12">
        <location>
            <begin position="425"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE95112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="477" mass="53299" checksum="D441E8F238209EAA" modified="2011-06-28" version="1">MIYIFGNGIAGLSAAVSLTKSGYKVTVITKKITGGSTYIAKGGIAAAVGSDDSPEIHAKDTLRVGDGLSDERAVNYVTSEAPKAVKTLEEWGFEFATDLRLEGGHSRRRVLHKTDETGRDIYDFLMRKAQELNIPIIEDELLAIKTENNEVKGFITRNRGEIEADKIVLATGGYAYLWKYTSNQSTNTGDGIAIAFRSGALVADMEFVQFHPTVTNLDGETFLLTETLRGEGAILLNDKGERFTFKYHEKGELAPRDVLSRAIYTEYLQGRKVFMDLTKIEDFENKFPVLNAYLKRHGKDKNFKIPVFPGAHFVDGGIRVNIRGESKIKGLYAIGEVSDTGLHGANRLASNSLAEGLVYGVNLPLYIDKWEGLYVDDGEIENVKLHIGSNLSIDEIREINWEDIGIIRNAEKLNKAISIYSSIDIESTNEKSNSALVSYLTALAAYKRTESRGNHYREDYPYKDDKWKKRIYFQISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I1</accession>
    <name>F4B9I1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_2256</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777042.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I1"/>
    <dbReference type="STRING" id="933801.Ahos_2256"/>
    <dbReference type="GeneID" id="10601762"/>
    <dbReference type="KEGG" id="aho:Ahos_2256"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_6_0_2"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17926">
        <property type="entry name" value="DEXHc_RE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18789">
        <property type="entry name" value="SF2_C_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040699">
        <property type="entry name" value="XPB_DRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18458">
        <property type="entry name" value="XPB_DRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="78"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="315"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE95127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="449" mass="51722" checksum="23B9E772E1D2D9CC" modified="2011-06-28" version="1">MVRLSYYKGLLLSDYYAPGLKWSDEFKKYIGFAYKYRDVLEYFKREEIEIEDNVLDTLPFPMIEDKITLRDYQAKALFFWNRAGRRGIIVLPTGAGKTAVGIKAITKVKTSTLILVPTIDLLDQWYEKIKDSLNYSPGRIGGGYDELKAITVITYDSAYTRVEEIGNKFAFVIFDEVHHLPSEGYSQVAQMLASPYRLGLTATPEREDSRHKLLPDLVGPVVFRLRTSDLAGKYLAEFEIKKIYVSLTDEEKKKYEELRKKLKQFLSSRNMKLDSLESFHKLVKLAGRDPEAREALLAWHTALRISVNSKAKIEKLRELLNEYCNEKENKIIIFTRDVEMAYNVSREFLIPVVTYKTSKDERSEILRKFKEGKYKVIVASSVFDEGVDIPDANIGIVLGGYGTSRQMLQRLGRILRKKEGEKNKALLIEIVTKGTSDYNLSKRRHRATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N5</accession>
    <name>F4B9N5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">L-iditol 2-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1067</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775867.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N5"/>
    <dbReference type="STRING" id="933801.Ahos_1067"/>
    <dbReference type="GeneID" id="10600541"/>
    <dbReference type="KEGG" id="aho:Ahos_1067"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_2"/>
    <dbReference type="OrthoDB" id="73567at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030554">
        <property type="term" value="F:adenyl nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043168">
        <property type="term" value="F:anion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08235">
        <property type="entry name" value="iditol_2_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="9"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="338" mass="37267" checksum="074D47FEBC0AE7E3" modified="2011-06-28" version="1">MKAIILENGRPVLKEVPIPKLNPGDVLVKMKACGLCGTDVEKICGQYTASQPILGHEPAGIIAESTVDWLKEGDRVFAHHHVPDYECYYCKKGSPTMCPYYRKTNLDPGGFAEYFRVPAWNVSRGGILKLPDNVSFEEGSFIEPLATVIRAQRRVFIDKDDYVLVVGAGPMGLLHVMMAKVNGAGKVYVSDISDFRNEYARKVGADEAFNPKVVKVEDEVKKLTDGRGVDVAIIASGAPSAILTALYSVRKGGRVLLFGVPYKGTILNYDISNLLNNEISIISSNAAVEEDTREALKVISEKKIDVMKLVTHKFRLEEFNEAVRIAKEGKTIKAIIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N8</accession>
    <name>F4B9N8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobQ</name>
        <name evidence="10" type="ordered locus">Ahos_1070</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N8"/>
    <dbReference type="STRING" id="933801.Ahos_1070"/>
    <dbReference type="KEGG" id="aho:Ahos_1070"/>
    <dbReference type="eggNOG" id="arCOG00105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001989">
        <property type="entry name" value="PRK00784.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="2"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="227"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="455" mass="50930" checksum="CD761BE346A3F042" modified="2011-06-28" version="1">MSDSGKSFVTAGLVRLLGGIPFKSQNMSLNSFPSEEGGEIAFIQAFQAIGAGLKPKSYMNPILLKPASSGKIEVIFFGKSLGNLRPEAYYEMIPSFWDKIKESIKENYVIEAAGGIEPNFMERDLTAFLPMKEGIPAVLVLDIDRGGAFSSAYGVYQMLPPSVRNSLKGFIINKFRGSPSLLENAIKWLTERTEMKFLGVIPYFDEELILPEDSMNIKDFGEGGETNIAIINYPYISNFNEFFALLKSNASVRFVNNPKKLRKDDIVILPGSKNTYESLVWLKERGFLEEIKKRQVLGVCGGFQIMGKKLIDPFGIESGEPREYDGLGIFDFVVKFEEEKVVSASEGLLQDGSIIKGYEIRRGRIVYEGNDRPLLTIVRRNGKEVNVADGSLKGDKLGLSIHGSLYSKNLLENFGVKIYSRSMEEEIVYMAEKIEKIIKQNVDIDEIKEIYTQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P1</accession>
    <name>F4B9P1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal-transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P1"/>
    <dbReference type="STRING" id="933801.Ahos_1073"/>
    <dbReference type="KEGG" id="aho:Ahos_1073"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09836">
        <property type="entry name" value="CBS_pair_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="72"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="14439" checksum="49E2C09C5D20D74E" modified="2011-06-28" version="1">MEEVVKEYMKSNVISVEKSLTLKEVAEIMTKNNVGSVIVVDHGKPIGIITEKDVVRGLGNGKDLNTKAEEIMTASLITIREDAPITGALSLMRTNNIRHLPVVNEDGKLTGILSIRDVARALDDMYENYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q1</accession>
    <name>F4B9Q1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA(Met) cytidine acetyltransferase TmcA</fullName>
            <ecNumber evidence="12">2.3.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tmcA</name>
        <name evidence="14" type="ordered locus">Ahos_1083</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a cytidine in RNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in RNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82211"/>
            <dbReference type="Rhea" id="RHEA-COMP:15704"/>
            <dbReference type="Rhea" id="RHEA-COMP:19834"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a cytidine in mRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in mRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15146"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>cytidine(34) in elongator tRNA(Met) + acetyl-CoA + ATP + H2O = N(4)-acetylcytidine(34) in elongator tRNA(Met) + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43788"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TmcA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.193" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775883.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q1"/>
    <dbReference type="STRING" id="933801.Ahos_1083"/>
    <dbReference type="GeneID" id="10600557"/>
    <dbReference type="KEGG" id="aho:Ahos_1083"/>
    <dbReference type="eggNOG" id="arCOG01951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004652_1_0_2"/>
    <dbReference type="OrthoDB" id="312894at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106162">
        <property type="term" value="F:mRNA N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01886">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024914">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 12" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="449"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="533"/>
            <end position="535"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="769" mass="88103" checksum="6A774CBF24B48B02" modified="2011-06-28" version="1">MQRNEFYEELKKALWDGKEKYYRNLVYIEKKDYLDDLLASIKTYLEVNQSPSVIYAFHPWVSKAKERRNIIKELFKEGKFEDVDYASTDEYLGNTYDLVILDLVDNFQPNYVGRLVDLARGGGLVILYTDNLTENKMFRNSIVRYGKILDIYERRFRKKLEEHEGIFIANEEGYKARPFVGNTKREEVKIPQKTLMPKELHELAISQDQIKVLEEFYFLLRGGKRVISLTAARGRGKSAVTGLGLAGIIKINLDEGYDTEVTVTAPSLYSASQIMEFAKKGLDVLKIKNKEKNSDTGFIREISGDDFEIYYKPPDAVSEVETGKGILVIDEAAALGVNFIDLALRKWKKIVLVTTIHGYEGSGKAFIRYLRKIISERKAPVRLLTMQKPLRYAEGDPIEKWLYDALVLNPEPQRLPEDISIAFYETQDKEELLMNDNKLFQVYGILVSAHYRNNPDDLMIMADGVHHSIKTIYSGDSYLAVVQTSEEGDLPDNLIDLALRGGTFDGDLIPDRLLKHVRLRDFGKLKGWRIIRIAVVPELQDKGLGSKILQMVTEDAREKGLDWIGSSFMGDYRVLKFWVKNGFIPVHVSPVRNEKYGDFPVVVIKPLSEIAKRIVDISASIFKEKLLNTIHDIYFNMSPQVAQLLLRGNKAYKDINLSKLHLAKIVAFLQGTSPYEASADGIHLLTLKYFWDSKRDWSLYDEEEWVLIGKVLQGKPWGYLSTLLGINRTQMNEFLYTAVATMLKKYYNMDAEGNIGYSLSDLGDEFTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T0</accession>
    <name>F4B9T0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1112</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775912.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T0"/>
    <dbReference type="STRING" id="933801.Ahos_1112"/>
    <dbReference type="GeneID" id="10600586"/>
    <dbReference type="KEGG" id="aho:Ahos_1112"/>
    <dbReference type="eggNOG" id="arCOG04415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="OrthoDB" id="146558at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="113"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="476" mass="52797" checksum="A1A499756F6C306D" modified="2011-06-28" version="1">MNEKKVLLIGDGARENALAEALSNNKVFAISSFINPGIKNVVERTGGKYFVGNINSKDFVKEIIKQVNPDFGVIGPEDPLFHGVSNAFYEEGIPVVGPIDKDAMIEKSKVWMRQLMWKYKIPGRLRFKSFSNLAEAAKFIIDYGGSVAIKPSEQVGGKGVKVVADIQAYLSNEKRQALSKSVDEIGKYVADDEVKVIIEERVDGPEYTLHVLTDGSSYLSLPLAQDYKHAYQDGIGPETGGMGSISGPKNLLPFINEEEYERSFNIVKMTAEAIEKETGEKYKGFLSGQMMLTDLWGPTIIEYYSRLGDPEASAIIPRIKNFGEILELMAEGKLSKAKIDLVEEPSVVRAIAPYGYPLNKSLAKGQKFHIDLQKIKEKGCILYFGSIALEGNELIAQGSRALELVAFGDFDEASKKLDECMAYISSDRKIIYRSDIGRTINEMVEKAEIVRYAYKNREKHGILGVSADWSPNGGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U8</accession>
    <name>F4B9U8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NurA 5'-3' nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777079.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U8"/>
    <dbReference type="STRING" id="933801.Ahos_2293"/>
    <dbReference type="GeneID" id="10601801"/>
    <dbReference type="KEGG" id="aho:Ahos_2293"/>
    <dbReference type="eggNOG" id="arCOG00367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_835811_0_0_2"/>
    <dbReference type="OrthoDB" id="33831at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053461">
        <property type="entry name" value="DSB_repair_nuclease_NurA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041033">
        <property type="entry name" value="NurA_Sulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="49"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="327" mass="37454" checksum="17C1FD99A1628FA6" modified="2011-06-28" version="1">MIERAYEEFANNYPKILEKIRFYSNSINDSVKDKLKKIWIDYEPTAVNKSFIAIDGGEFVKELRVGTVFLTNAEAVYGEGVNVSAIDNEIKMGVFRPGNLAKERISEIMNILELSLALRNGNKAEYILMDGSLKKKLNNGKDVEGEEKELDEIINDENEEESLKNLILKSQILISRLIQNYDGKILWISKNSKGRELFGENISDITVIESLTENPGFTLPFVHTIVGKILAKNKEVKNLEGVEITSFYIRLEKGQRVLKVDLTGRIDDEGIKKIMDSLYSVSIKGYPYPLLKVHYDVKVNKEDRQRILNILGLNHMRGNSWWPNQFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W0</accession>
    <name>F4B9W0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777091.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W0"/>
    <dbReference type="STRING" id="933801.Ahos_2305"/>
    <dbReference type="GeneID" id="10601813"/>
    <dbReference type="KEGG" id="aho:Ahos_2305"/>
    <dbReference type="eggNOG" id="arCOG01991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_3_2"/>
    <dbReference type="OrthoDB" id="304253at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054929">
        <property type="entry name" value="dPGM_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038349">
        <property type="entry name" value="dPGM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="23870" checksum="46FB577F27E5656D" modified="2011-06-28" version="1">MVLVLFVRHGHSVSNQNKILSHDVNNYPLTEEGQSQAKNTAKELLKLKITKIFTSPILRAYQTATIIANELGLIPIIDDRLRERYLGDLNNKKFDPSDHWKIKLIKGQIEVKGLEPWESMQRRMIEFVNSIKNEEGAIVAVSHYDPIRALIGYILGLDDISAFGISLPNASITAIEMIDGKFRIHSIGSPILSQELLKRLNRYIITINQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X7</accession>
    <name>F4B9X7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NAD kinase</fullName>
            <ecNumber evidence="8">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nadK</name>
        <name evidence="9" type="ordered locus">Ahos_2322</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777108.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X7"/>
    <dbReference type="STRING" id="933801.Ahos_2322"/>
    <dbReference type="GeneID" id="10601830"/>
    <dbReference type="KEGG" id="aho:Ahos_2322"/>
    <dbReference type="eggNOG" id="arCOG01348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_3_2"/>
    <dbReference type="OrthoDB" id="77798at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF43">
        <property type="entry name" value="BIFUNCTIONAL NADP PHOSPHATASE_NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="6 8" id="KW-0521">NADP</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="110"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="149"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE95193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="27209" checksum="8725BB88385C5C22" modified="2011-06-28" version="1">MRIKIVSKPSSQILEIVQKIITTAKSQGFEIDEQNPDIIIAVGGDGTLLKAIKLNKPVIAIKAGRRGFLMDVNPESIDEVFKRLKENDYKIQEYPLLETNICGFSSLAFNETGILADQPETILLTLSFLNSEIQIEGDGVLISTPQGTTGWSLSATGNILYGINAMEVAFINPVLSPLRSIVLPPTEIKVKVESKGYPQKIRVTSDGDLLKVINEGSEIKVRVSPKKAIIYRFFDIDPLRRILNGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z9</accession>
    <name>F4B9Z9_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10601852"/>
    <dbReference type="KEGG" id="aho:Ahos_2344"/>
    <dbReference type="eggNOG" id="arCOG08538">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1471999_0_0_2"/>
    <dbReference type="OrthoDB" id="43902at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="183" mass="20350" checksum="0BB1F1ADC221FC64" modified="2011-06-28" version="1">MIPILLPLIIASLAPHSSLIYAVQVNVNNTIYCITYNFTVICVKNNTIEFNLTITGNNYYNVMQYNVNEDDPYPLPINCVAFNTTNLKFIGNTTLGGNLTKEFSGIFNALGKYRVPVTVYFHDGILQQLNGTYNGVSVHVSREYPTIISTTPSSESLSNYVTLIIFIVVLIIAIFVLIKIGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA18</accession>
    <name>F4BA18_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DHOdehase electron transfer subunit t</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1131</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775931.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA18"/>
    <dbReference type="STRING" id="933801.Ahos_1131"/>
    <dbReference type="GeneID" id="10600605"/>
    <dbReference type="KEGG" id="aho:Ahos_1131"/>
    <dbReference type="eggNOG" id="arCOG02199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318587_0_0_2"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="194" mass="22273" checksum="C9CFCE7528E75C48" modified="2011-06-28" version="1">MIYGKILQIKEKKTIFEVSIEVHFNPLPGQYVSLVFPSENEIPLGIGDYYDGVLSLFIESEKIVNKIRNKKFVILKGPLGKRIDLGRRILGIAEGNLYYDLLFPLRQAKRQGKEVKVLCKDCESEFEEPSRDEDFDMILASVDYNEIKYLPPEAYVYVRWVKMNCNMGVCGICNINGHLPCIEGPFMKVKDLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA19</accession>
    <name>F4BA19_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA19"/>
    <dbReference type="STRING" id="933801.Ahos_1132"/>
    <dbReference type="KEGG" id="aho:Ahos_1132"/>
    <dbReference type="eggNOG" id="arCOG00689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001541">
        <property type="entry name" value="PRK00369.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="186"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="335" mass="38057" checksum="4966E82AFFF00D90" modified="2011-06-28" version="1">MHVHVRGMNLSYKEDVRTATSEAAYGGVTVVVDMPNTVPYINTAERVQERLREFANFSRADYGIYSGVTSDERVDKLPIAGYKVFPEDLEKPELQFVLSSKRLKILHPELPLSTKQFRNLREMWQEIASISLVSGRFHITHITNYEDLIKAKELGFTTDFTPHHLLLEEIKGDCLTKVNPPIRDLTERRKLLKALFEADAVASDHAPHTLQEKMQPYELCPPGIAAVSFTTPFIYSLVKKGVLSLSRAVDLLSKNPAKILGINAGEIKEGNVADFTVIDFEKDWRYHTQYSKVVETPFDEYSLDVSIYMTIVEGKVAYDGYDVYPIRGMNLFENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA65</accession>
    <name>F4BA65_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1179</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775978.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA65"/>
    <dbReference type="SMR" id="F4BA65"/>
    <dbReference type="STRING" id="933801.Ahos_1179"/>
    <dbReference type="GeneID" id="10600653"/>
    <dbReference type="KEGG" id="aho:Ahos_1179"/>
    <dbReference type="eggNOG" id="arCOG04138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025558_0_0_2"/>
    <dbReference type="OrthoDB" id="85892at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="644"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="720" mass="81844" checksum="349D0C6C79EA7816" modified="2011-06-28" version="1">MILPEKMSRVQIIANKELLDDIVTKILKFQNFEPEEPEEPISNERFEEARRKLGIIQEHLNKFQIIMDLAGVTIEPKGKMKAGNWNKIADEVDNDATQEEEKYKELLEEIGKIKSELDLYKAQLNEVLPFKDITVNLSKLYNLKLFDVYLLTILSSQLDKVKFDNALVLTKRINEKTYAVIIIAPKGVLQKDKIEKEIGAKVFETPEGKAPYDVYNEVQNKINELTKILEETRAKLKEKLRACEIHVKEIYGRLLTVRDALTIISRARVSEFYVQIEGYAPTKMVKKLKDQLKGEAFITERLPRRYGDKDKPPTLISLPKSIRVIESVVELYGTPSYWEISPIIFLIFTFPILFGLMFPDFGNALVVFLFAIWFYKYGKRKGSENTEKLSLVLIYSSIVAMITGLLAREFFGPVLVGGPREVFNNNSYPVGPLYYVWPVPVSVSDSLKYLIPFGNYSILSVEIEDTMILSIFIGALALFVSSLLGVINAIDKKDPEFLFYEKLPLLILYTVPLIIFGYGFVDINDYFGKVECLLGGLLTNIFSFPPNLSTPTYALAYILILWVEIGLIYNWISKVILIKRHEGHVGLGAAIGMGFIEGGFEAGILLLSNTISFIRILVFALAHYYLLYAFSYMGLLVLYSSVPYVAAVIIAGIIIALGNLLAIALEGLIVFIQDMRLHFYEMFSKFYEGQGRKFMPAMNYVELEEEKEVIKGEVIEVKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA75</accession>
    <name>F4BA75_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH/Ubiquinone/plastoquinone (Complex I)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775988.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA75"/>
    <dbReference type="STRING" id="933801.Ahos_1189"/>
    <dbReference type="GeneID" id="10600665"/>
    <dbReference type="KEGG" id="aho:Ahos_1189"/>
    <dbReference type="eggNOG" id="arCOG01540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_1_4_2"/>
    <dbReference type="OrthoDB" id="29144at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004445">
        <property type="entry name" value="PRK05777.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22773">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="6">
        <location>
            <begin position="118"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="457" mass="50298" checksum="865202D7FEEC2DBD" modified="2011-06-28" version="1">MFTQDLPIIILSVLFLFSAIAVLFIKEFTRAFYLSLSVNIIGIIILSFFWIIGFVDYTIFSCTLYLDNVGYLFSIIVLIGTTVVILGGKSHIENWSTKSSMLSLLLLTGLGLVYMAFAYNILIILGAWGISSAASYAITMLRKDYKSVDAGIKYLIMGLISSSFMIFGFALYFLSVNTFSLNYSTIKYPYLFMLGISLLSVAFFFKLGAFPFQGWLPEVYINADRISVSFISSVGKLLGIIPLLRIISLGDPTQNVITFFVVLFSIMAILSMFVGNITAFSRKDLPSILAFSSIAQMGFILIGFVALKVNTQIAEAGLVTQSLAYVIAQAGLFNFVSHIEKVSGTARFEGLRGLAKSDKGLATSASILVLSLLGIPPIIGFWGKLFLFESVYFYPWLIIIAVLNSAISAGYYIPIIREMFREGELNFVESEERDSVIFSAILSIGIGIISPLILVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L4</accession>
    <name>F4B3L4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Band 7 membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774820.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L4"/>
    <dbReference type="STRING" id="933801.Ahos_0008"/>
    <dbReference type="GeneID" id="10599448"/>
    <dbReference type="KEGG" id="aho:Ahos_0008"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_3_3_2"/>
    <dbReference type="OrthoDB" id="10752at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08826">
        <property type="entry name" value="SPFH_eoslipins_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="22"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE92903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="265" mass="29469" checksum="7B3B0372E7977344" modified="2011-06-28" version="1">MDTGLIIGLVLLLIIILIFVGMSLRQVKEWERAVVLRLGRILGVKGPGIIFLIPFVDRPVIVDLRIVTVDIPPQTIITKDNVTISIDAVVYYKVLDPIKAVSMVYNYRSAVLNISQTSLRDIVGQMELDEVLSKREEINKKLQEILDNYTEAWGIKVTAVTVRDIKLSPDLLSAMARQAEAERQRRARVILSEGERQASTILAEASQAYKNNPAALQLRFLETLSDISQKGGLIIVVPAGQELYPTISLASFAASYVKQQEIGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M5</accession>
    <name>F4B3M5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional activator, TenA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774831.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M5"/>
    <dbReference type="STRING" id="933801.Ahos_0019"/>
    <dbReference type="GeneID" id="10599459"/>
    <dbReference type="KEGG" id="aho:Ahos_0019"/>
    <dbReference type="eggNOG" id="arCOG01128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_3_0_2"/>
    <dbReference type="OrthoDB" id="85443at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="10"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="212" mass="24905" checksum="C784DD4F505FE80F" modified="2011-06-28" version="1">MNSDILREKAGKLWENYVKHEFVIKMKEGGLDLESFRYYLIQDSKYVEMMLKSLLRASSLAPLSYSTKILKSIFDTRDKGMEVHSWLLSRLGISHEEIQNTGYSLVNYAYTRHLYYYSTKSWEEFLAAWAPCMWGYYEVGKFVKDSPNELYSKWAEFYASDVYKSRVDVILEALNSIKYSESLVTPFINSVKFEIMFWESALKKEPTLIIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P8</accession>
    <name>F4B3P8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleotidyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776001.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P8"/>
    <dbReference type="STRING" id="933801.Ahos_1202"/>
    <dbReference type="GeneID" id="10600678"/>
    <dbReference type="KEGG" id="aho:Ahos_1202"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_0_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="3">
        <location>
            <begin position="245"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="356" mass="39832" checksum="E47D2AFD10CAD261" modified="2011-06-28" version="1">MVSAIILAGGYATRLRPLSLTKPKALFPVLGKPILDYILEGLENAGIHNVYLSLRVMADKILSHVDGKNVTPIIEKEPLGDAGALKFVSTQANLDDVVIVIYGDIYSEVNFLDLLKFHAQSECPVTLLATKVDNPRRYGVLLTENNKLIEIIEKPSNPISNLINGGVYVFNKDILNFIQGPSISRNFLPKILEKYCVSVYKYDGIWADIGTPYDYMKLNIELLGKRFPRGYISNTAKVSERTTLTPPYFISDGVIIYEDSYIDSNSIIGKGSVVKNGVYIGESLLMENVFVNENSFIKGSIIADKCKIGKWNHIREETIFGEEVITYDGILINRKNIILPNKEVTESIYEEDKIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U4</accession>
    <name>F4B3U4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="11">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">priS</name>
        <name evidence="14" type="ordered locus">Ahos_1248</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776047.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U4"/>
    <dbReference type="STRING" id="933801.Ahos_1248"/>
    <dbReference type="GeneID" id="10600727"/>
    <dbReference type="KEGG" id="aho:Ahos_1248"/>
    <dbReference type="eggNOG" id="arCOG04110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056123_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001641">
        <property type="entry name" value="PRK00419.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20873">
        <property type="entry name" value="PriS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 11 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 11 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="313" mass="35344" checksum="8082C078DD2E6A29" modified="2011-06-28" version="1">MLFKSYYEKAELELPDDMELREFAIQPFGKDTYVRHLSFSSPLELKNFIIENLPLHLFYSTAKYQIPSAKEMDEKGWMGADLQFDIDADELCEVRKINFCPVCGSEVDGEKCPKDNVETVEFTEITSDCINKALENALIIKDILKEDFGLNPELYFSGNRGFHVYVRCSGDCALLDSEDRKEIVDYIKGIGVPKYSSALPTDPGWAGRKAKGIQGVILDEEVTIDVKRLFRIPNSIHGKSGLLVRKIEESNFEFSTSLSPFSGYVTFLPYISGKFQILGENIEFSRGIPIKLDASIGVYAHLKGLGEVKLYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Y6</accession>
    <name>F4B3Y6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774867.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Y6"/>
    <dbReference type="GeneID" id="10599495"/>
    <dbReference type="KEGG" id="aho:Ahos_0055"/>
    <dbReference type="eggNOG" id="arCOG05906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113587_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="24357" checksum="EC6740CD74F2D051" modified="2011-06-28" version="1">MKRDNYTKDLHNIKYRIISNYGNLPQIDEIYKRLIDFHKLGFVNITHSALELIVAAYLIKEGFKVSVEHESNGKIIDVYAIKGIDVGIEVETGFVPPSFAGKQEDFLMSRMALKIARYSNLASQFYIAVPSYYIPPVPVELLKDKEKRSEDEIRNLMRLIRKYHNSLDVNLLSLKSAKIDGIIIINTTDLKLKIFDYEDFLKIKKYYSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B411</accession>
    <name>F4B411_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774892.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B411"/>
    <dbReference type="STRING" id="933801.Ahos_0082"/>
    <dbReference type="GeneID" id="10599522"/>
    <dbReference type="KEGG" id="aho:Ahos_0082"/>
    <dbReference type="eggNOG" id="arCOG10432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044272_0_0_2"/>
    <dbReference type="OrthoDB" id="41787at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="120" mass="14567" checksum="CB70ABAC5FA59C20" modified="2011-06-28" version="1">MKINDLTRIIEDLGFHIERIENIERGIRIFIKENFYIDIIEENNEWILYYVRKVECKNLHLIREKIKKRNQEILNRGYITGIYFLSNSCNIVVVKKLKNGKDEKLWDEYFNMIKIADLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D0</accession>
    <name>F4B4D0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acylaminoacyl-peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774936.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D0"/>
    <dbReference type="STRING" id="933801.Ahos_0126"/>
    <dbReference type="MEROPS" id="S09.071"/>
    <dbReference type="GeneID" id="10599566"/>
    <dbReference type="KEGG" id="aho:Ahos_0126"/>
    <dbReference type="eggNOG" id="arCOG01646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_2_2_2"/>
    <dbReference type="OrthoDB" id="25019at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69304">
        <property type="entry name" value="Tricorn protease N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="378"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="587" mass="66794" checksum="9556BC2911FDB4E9" modified="2011-06-28" version="1">MTPEEAYSIKLISDVKLNNEGDLLHVETWIENKEYRSAIFLNKRKIIEGKVSLPRFNGEYLYYVKEVKGQKNKKEACLFRKSPYGEEEKLLCLGKISDYTFHNKGILILGEDKVNKNKPFEATKLKYRFDSRGLLRTRQSLYLFSTKLEKIISGDFDVTGVATNGKRVVISATKENDDIGLSDVYEVYLSNGEMRRITQGEGIVNALAMNEKGQIAYLGHRKGRAPWAVEEIILPEEDKSFLCGNTCGGDVLTDLFDGAKTRIYFVNDVIYSLGQVKGEVQIYKISDKSEQITEGKIVVRGFDVNAKEDIAYFYTTPTKPSILHYKEDYDSNPGVEGIEPQEINDEIQGWAIITNPNNPTILFIHGGPHAAYGYGYFIEFQFFASNGFNIIYCNPRGSQGYGEDFAKACVGDWGGKDMQDIINFTKKVIEKYNLKGKLGVTGGSYGGYMTNWIITQTDMFSAAIAERSISNLVSMCGTSDIGFWFNAIEAGIDDPWKQENIEKLMRMSPIYYVKNAKTPTMLIHGEEDYRCPIEQAEQFFMALKMNGVPTVLERYPGDSHEHARRGKPKNMIDRLYKKIWWFTKYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D7</accession>
    <name>F4B4D7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DZANK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774943.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D7"/>
    <dbReference type="STRING" id="933801.Ahos_0133"/>
    <dbReference type="GeneID" id="10599573"/>
    <dbReference type="KEGG" id="aho:Ahos_0133"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965096_0_0_2"/>
    <dbReference type="OrthoDB" id="53394at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DZANK-type" evidence="2">
        <location>
            <begin position="265"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="319" mass="34897" checksum="C4E577626BC39AFE" modified="2011-06-28" version="1">MSYPYGNPYYPQGGYPPQGYPQGNPYYQQNSNFAFMMCAQPIGLGGKQMMIPINHPIDLQYVAQQAVMYLMGQGFQAYPMVGQNMAVIQAQHMSLLGDLTAGNKAYTIRICEGQGFVMVETGITNLMQDLLTVAATAGGTYLLGDDVLHSKLVELAGGGATALDLYHLYQDYANEEQLMNIIMMLVTTAPPPPGYPVGQPGYPQPYGQQPYGQQGYPQQYPPQQYGQQYYPPQQTALQNPVQPTTQQNAVQPTPQRTVQQKMVKCWKCGEEIPEGSKFCPFCGAPQTPVKCPKCGTINSPGAKFCSNCGAPLQVEKQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E2</accession>
    <name>F4B4E2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774948.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4E2"/>
    <dbReference type="STRING" id="933801.Ahos_0138"/>
    <dbReference type="GeneID" id="10599578"/>
    <dbReference type="KEGG" id="aho:Ahos_0138"/>
    <dbReference type="eggNOG" id="arCOG10299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922649_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="15203" checksum="240392ADEE856098" modified="2011-06-28" version="1">MNLFRFLIYISLFLLSIQFIDGMWLNLFVQFSPFTGMMNFMFNYGPIFMFHVFIGFILFILSLIEFVAFTRNQPIGVFLPLLNLLSIIMAGISGMLFMVTGFSNNYYSFIMAIGFIISIMSNSLMLVKVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J8</accession>
    <name>F4B4J8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775004.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J8"/>
    <dbReference type="STRING" id="933801.Ahos_0195"/>
    <dbReference type="GeneID" id="10599635"/>
    <dbReference type="KEGG" id="aho:Ahos_0195"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_1_1_2"/>
    <dbReference type="OrthoDB" id="101044at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="11"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14657" checksum="41F4A36640D7BAC5" modified="2011-06-28" version="1">MSSNDDVNLLLKRAEKFRKDSITAYQEGYYDISCFYAEQAVQLKAKAFILKNLGFMPRIHGIRELLSIIYKYTGNENIKNFIDKNRRELKELEEGYTDSRYGFSPYTKEDAEVCLNLMNSIFNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L9</accession>
    <name>F4B4L9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1366</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776164.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L9"/>
    <dbReference type="STRING" id="933801.Ahos_1366"/>
    <dbReference type="GeneID" id="10600853"/>
    <dbReference type="KEGG" id="aho:Ahos_1366"/>
    <dbReference type="eggNOG" id="arCOG04269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="OrthoDB" id="6609at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136:SF3">
        <property type="entry name" value="DNA REPAIR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal Nre N-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Archaeal Nre C-terminal" evidence="3">
        <location>
            <begin position="304"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="410" mass="47187" checksum="447C494490E936E9" modified="2011-06-28" version="1">MRAIPAELCVKCKGNKLLCGLRACPILERFRSTINVVNKVYNKNEIEGSTPPSIVIGEKNYPKVSISFNIPPDIYGEEAKNYENPKGWWGKASIYDIINYRTSLLSNVFEVKIEDVWKLYEKELSLAAVSEKPVQSESRIDGTILPKLRFDGYVLPRGPSAKTQNLEVIENPKINKTLDKLIFDDIKAEEAVVKLYQDIKDVYTIINALSLGLLGVKKNRKLVPTRWAITAVDSIIAKNLLQKIRNYQEISEVEVYYQKYLGNYFHVILYPSAYQVTWIEIWHPLSLWASELTISELTENYWGEYSTMDGGYMAARLSVLEYLDNIKRSAGVIIIREITSEYFAPLGNWHIRETVRKAMENKIGRFQDLENALSFVNQRLNAKKIHLRELNSIKRILKQKSIDQFFSSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T4</accession>
    <name>F4B4T4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-cone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775016.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T4"/>
    <dbReference type="STRING" id="933801.Ahos_0207"/>
    <dbReference type="GeneID" id="10599647"/>
    <dbReference type="KEGG" id="aho:Ahos_0207"/>
    <dbReference type="eggNOG" id="arCOG03714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431715_0_0_2"/>
    <dbReference type="OrthoDB" id="30964at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="4">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="22030" checksum="6ABBCCEEB1A61886" modified="2011-06-28" version="1">MTKVIKRSGEEEEYLSDKLYNALIRAGASDEVAKEIVKEIDERVKEREKISTDEIRRYVLTRLQQLEPEVADAWQFYDRVFKGRITFENGKAIVVDKGKLYLGRKVKDFNGKGLENAEQVKEILDELKEDMDYGLSPKVINARLYALFMGVLHKKDMPESEKEKAIKYINDFRESLGWKPYELKYPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U7</accession>
    <name>F4B4U7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heavy-metal chelation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4U7"/>
    <dbReference type="STRING" id="933801.Ahos_0220"/>
    <dbReference type="KEGG" id="aho:Ahos_0220"/>
    <dbReference type="eggNOG" id="arCOG03216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076326_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159713">
        <property type="entry name" value="Dhaf3308-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="20"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Putative heavy-metal chelation" evidence="1">
        <location>
            <begin position="113"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="28801" checksum="3A634E016C679083" modified="2011-06-28" version="1">MIKHKSYCYYYFIMGSKVIESLISYAKENDCEIKNATVGVTWTCVLSKYCGVSMSYNTGNEDLDVKGFGYLEEKRVGELAEYLRSWNLLEASVGLAAINSVIEPKGNTEANGLDVAIEIGKGKKIVMIGKFPGLQKFKEVAKEFIVLELNPFLIDPSQGILPSTAAETVIEDAQVVIITATTIINKSIDRLLELSRRANAYVILIGPSTPMLDLMFDFGVDVLAGIKVNRPYSFIKKISQGCGMVNPSKLEGDVSFIIRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Y8</accession>
    <name>F4B4Y8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoside-triphosphatase Ahos_0262</fullName>
            <shortName evidence="4">NTPase</shortName>
            <ecNumber evidence="4">3.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleoside triphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0262</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the THEP1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775070.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Y8"/>
    <dbReference type="STRING" id="933801.Ahos_0262"/>
    <dbReference type="GeneID" id="10599702"/>
    <dbReference type="KEGG" id="aho:Ahos_0262"/>
    <dbReference type="eggNOG" id="arCOG01034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103145_1_0_2"/>
    <dbReference type="OrthoDB" id="52698at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19482">
        <property type="entry name" value="RecA-like_Thep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010248">
        <property type="entry name" value="PRK13695.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146:SF1">
        <property type="entry name" value="CANCER-RELATED NUCLEOSIDE-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="166" mass="18562" checksum="D55787EA4C9A9571" modified="2011-06-28" version="1">MRVFITGKPGVGKTTILMYVVKELIKRNYTVVGFYCPEVREGGRRIGFKIVSIPTGKERWLAKVGEGKIKVGKYAVQDEAEEVVNDVKDSLPKAQVIAIDEIGPMELSMPSIKSLIDEVLKLEKPLIAVVHRNVKLNVDGKTYIVTEENRGLLKEKILEEIVRTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B506</accession>
    <name>F4B506_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxyisobutyrate dehydrogenase</fullName>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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    </evidence>
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</entry>
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            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
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            <dbReference type="EMBL" id="AEE94323.1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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    <accession>F4B558</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Wang S.Y."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </reference>
    <comment type="function">
        <text evidence="7 11">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 11">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="9 11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776275.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B558"/>
    <dbReference type="STRING" id="933801.Ahos_1477"/>
    <dbReference type="GeneID" id="10600976"/>
    <dbReference type="KEGG" id="aho:Ahos_1477"/>
    <dbReference type="eggNOG" id="arCOG00039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550565_0_0_2"/>
    <dbReference type="OrthoDB" id="6532at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00352">
        <property type="entry name" value="Gn_AT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="QueC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00364">
        <property type="entry name" value="7-cyano-7-deazaguanine synthase QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="13">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="12">
        <location>
            <begin position="238"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="463" mass="52210" checksum="309AB89DCA57CA09" modified="2011-06-28" version="1">MCSVSGALILNSKNYDKIERKFAEILKNAEDRGKDSFGIIVVQSDGTTKSVKSLGRPSEQEEKLYGILDEKSRVVIANNRAEPTTEFVNKKTEKDIQPFEGERFIVTHNGIIANDKELEKKFGVKRESNIDTAVIPPILDKFWKGDLPTLKDLLSEIRGSFAFIITDKKNPNEIYIAQNFKPVYMMYDYELGAIFFTSLDDYFSQNPLDKVNITKLEPYSIVSVDSNLKIEKIDLIKPKDKKKVLVIASGGLDSTVAATYLLRQGHEITLLHFNYRHKAEEKEREAVRKISEYLQVPYIEIDTDLFKIIGHTTLLKGGGEIVKERQGEEGAEFAHEWVPARNLIFFSVALAIAEAYGFDAIASGINLEEAGAYPDNEMEFVRLFSKLSPYATGPNKKIEVMMPVGNLVKHEIVKLGVEIGAPLHLTWSCYEGGEKHCGKCGPCYMRKMAFKINGLKDPVEYEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B566</accession>
    <name>F4B566_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1485</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B566"/>
    <dbReference type="STRING" id="933801.Ahos_1485"/>
    <dbReference type="KEGG" id="aho:Ahos_1485"/>
    <dbReference type="eggNOG" id="arCOG01339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000006">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="4">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="258" mass="27673" checksum="317F7F4B4583ACB9" modified="2011-06-28" version="1">MLEYGTKIVAGVTPGKGGSQVYGVPVYDTVKEAMQEHEIDASIIFVPAKFAVDAIYEAIDGGIPLIVVITEHIPVLDMAKVVHYAEKKGVRIIGPNCPGIIVPGESLLGIMPSRAFRKGKIGIVSRSGTLTYEVAEILKDFGQSTVIGIGGDPIIGTKMQDIVKMFDEDKDTEEIVIIGEIGGTMEEEVAKMKKEGKISKNIVAYIAGLTAPKEKRMGHAGAVVYMGLGTYESKVNAFKEAGIKVAKTPFEIKDLIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B569</accession>
    <name>F4B569_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Purine or other phosphorylase family 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B569"/>
    <dbReference type="STRING" id="933801.Ahos_1488"/>
    <dbReference type="KEGG" id="aho:Ahos_1488"/>
    <dbReference type="eggNOG" id="arCOG01324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068457_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17764">
        <property type="entry name" value="MTAP_SsMTAPI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF11">
        <property type="entry name" value="FI09636P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="4">
        <location>
            <begin position="15"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="236" mass="25671" checksum="648C960F12DD1FDE" modified="2011-06-28" version="1">MPVHILANKGDVAERVLIVGDPGRAKLLSSLLQNPRLVNENRSFLVYTGKYNGVDISIATHGIGGPSAAIVLEELIMLGGRTFIRYGTTGALIPEINLGEYIIVAGASYNHGGVIYQYLREDSCIAATPDFYILNSLVQNFQKEGLKFHIGNVFSSDAFYAEDEEFVKRWSSRGNIAVEMECATIFAVGRMKGVRTGAVLVVSDNLAKGGVWISREDLENSVMRGAKAILDTLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B574</accession>
    <name>F4B574_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ArnR1-like winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776291.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B574"/>
    <dbReference type="STRING" id="933801.Ahos_1493"/>
    <dbReference type="GeneID" id="10600993"/>
    <dbReference type="KEGG" id="aho:Ahos_1493"/>
    <dbReference type="eggNOG" id="arCOG01055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159725_2_1_2"/>
    <dbReference type="OrthoDB" id="34509at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArnR1-like winged helix-turn-helix" evidence="1">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10943" checksum="78D416BEE78551CE" modified="2011-06-28" version="1">MKSKRDQLEIIMDVMEVINDGYGSKSSIMKYANLSNTLLEKYISILTSKGLIKYEDGHYKMTEKGIELLEKLRKIRKLHLELSELINSVSEELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B583</accession>
    <name>F4B583_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775093.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B583"/>
    <dbReference type="GeneID" id="10599726"/>
    <dbReference type="KEGG" id="aho:Ahos_0286"/>
    <dbReference type="HOGENOM" id="CLU_2079283_0_0_2"/>
    <dbReference type="OrthoDB" id="18576at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="13057" checksum="9D00374F77A19118" modified="2011-06-28" version="1">MKHRIFTYIIFMILLASLSSTLMLAQSQGYKVNINVSSSGKIEVCLYSPVTSGEVGDAIIPISHHGFLIQKTTIEQGLCFIKVDKIEYNTQPEFMRLPLILLVAIIVVFVEKGLKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B591</accession>
    <name>F4B591_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pirin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0294</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775101.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B591"/>
    <dbReference type="STRING" id="933801.Ahos_0294"/>
    <dbReference type="GeneID" id="10599734"/>
    <dbReference type="KEGG" id="aho:Ahos_0294"/>
    <dbReference type="eggNOG" id="arCOG02935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_5_0_2"/>
    <dbReference type="OrthoDB" id="23530at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="3">
        <location>
            <begin position="20"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="4">
        <location>
            <begin position="185"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="294" mass="33227" checksum="5F812211FB61C723" modified="2011-06-28" version="1">MIRKVSGLIQGKYTYDGAGVKLYRVFGATSTYHLTDPFLLLDFFGSSNVEDYINGFPWHPHRGIETVTYLLQGKVEHEDSEGNKGVIYPGEAQWMTAGSGIFHQEMPKPLDESDILHTRILTTENKGLQLWINLPANKKMTSPIYRDIRRIPKVKIDKGEVKVLSGEFMGVEGPVNAGIDVDPTYLDIKLEEEGEFRHGVHHKYTVLAFIIEGSIDFPVKVGEGNLLIFDEGDEIYIKSKGGARLILLSGKPLREEIAWYGPIVMNTEEQIIEALNDLRRGTFVKDKKVERITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J5</accession>
    <name>F4B5J5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776334.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J5"/>
    <dbReference type="STRING" id="933801.Ahos_1536"/>
    <dbReference type="GeneID" id="10601036"/>
    <dbReference type="KEGG" id="aho:Ahos_1536"/>
    <dbReference type="eggNOG" id="arCOG14121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536657_0_0_2"/>
    <dbReference type="OrthoDB" id="42294at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="175" mass="19411" checksum="B4C58588663F1067" modified="2011-06-28" version="1">MDEVAFLASFLPCSFEGFEMSLFSYIATSNNRKMGNLGTLTGVLLVLSLIYLTYIFLPVLITDTSEYIIRMSLGFLLIGMATLFFFRDYPEPRGAFLTAALGITAEGIEVDTFTVSSWIMTGDYTWALIGGILGFMWSLIAFRIISVKFPKRIMKNVAVGLLYTVGFIILTSGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K7</accession>
    <name>F4B5K7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved Acidianus plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K7"/>
    <dbReference type="STRING" id="933801.Ahos_1548"/>
    <dbReference type="KEGG" id="aho:Ahos_1548"/>
    <dbReference type="eggNOG" id="arCOG03737">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691445_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="155" mass="18088" checksum="889B157D557FC75B" modified="2011-06-28" version="1">MGTLKDRTVIDKIRYIGSNMDKLTEEERKEFTNWLNELYNAVSSILGKNSNMSLSNLGNAELKEINEKLDKVLEILNETKKRKKTTYDLDVLYNKMKDSLGFVRIDNLRKELGMSFEEFMQTFGDYIEKHYELIPGGDEGFIRNGVKYGIIRRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5L4</accession>
    <name>F4B5L4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PNOB8-type archaeal integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776353.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L4"/>
    <dbReference type="GeneID" id="10601055"/>
    <dbReference type="KEGG" id="aho:Ahos_1555"/>
    <dbReference type="eggNOG" id="arCOG01247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_635569_0_0_2"/>
    <dbReference type="OrthoDB" id="42022at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00397">
        <property type="entry name" value="DNA_BRE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="227"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="431" mass="50471" checksum="FD8B4075132BA2B6" modified="2011-06-28" version="1">MAVIDVSKLTKEQRIKLLEKAKEKLGMSKLEEITSRSRKQIYIYLRGFDERGKEIIIPGEVIEKITQALPIDQIYEVLHGFNPREVTINDAIAVITRAVQDPGFRNIFFMLLQNQFGEYLKETSTSYLVTSADIELFEKLIRNKKSKKTADDYSRYIRRAFADMGYELSPEKLKEYILDLEEESIGKAKHTAKALKLFIKEIVKSKNSMLARELYESFKIPKSKPSHKPFIPDIATVKQIFNNIKDIGAKAFFLLLTESGLRVGEVLSLKLEQVDFEHRIIHINKISETKRAYITFLHESTAKWIKEVYLPYREDFIRKYESSLRKLASANPEQGINVDEWKTKLFPFREANLRVEIKEAMKKVLGKEFRLYNMRAFFTSYMIKQKVPGTIVNLLQGRASPQQFKVMEENYFVISEIELREWYDKYAPRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P8</accession>
    <name>F4B5P8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRISPR-associated protein, Csa1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775179.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P8"/>
    <dbReference type="STRING" id="933801.Ahos_0372"/>
    <dbReference type="GeneID" id="10599813"/>
    <dbReference type="KEGG" id="aho:Ahos_0372"/>
    <dbReference type="eggNOG" id="arCOG04195">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_905009_0_0_2"/>
    <dbReference type="OrthoDB" id="19284at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009260">
        <property type="entry name" value="CRISPR-ass_Csa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01896">
        <property type="entry name" value="cas_AF1879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06023">
        <property type="entry name" value="Csa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009226">
        <property type="entry name" value="UCP009226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="293" mass="33882" checksum="21452FC1613C3A1C" modified="2011-06-28" version="1">MFFTSSDILLLSRRIKKSPRAIDEELRGWNWNEPPIYSQHFSQTSVSELIYCSTLRNLYLRQKGYKVGEESSLIKKGRAIHDTYSTAISTIKRLLYSSPQIDGNKLKTLMTDEFYSFLKKYEDQADYVKTLWDYITNIYSAELDRVRSKFFNLTEDSLVSSVVPFYVEYPVDGSLVGLSSSLRIDAFVPFIPLIAEMKTGKYKYSHELQLAGYALAFESQYEIPIDFGYLCYVNVDEKEVKSNCKLIQISNSLRSEFLDKRDKALEIMDNVIDPGIANDCEKECPYYKVCHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q3</accession>
    <name>F4B5Q3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cas2</name>
        <name evidence="9" type="ordered locus">Ahos_0377</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775184.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Q3"/>
    <dbReference type="STRING" id="933801.Ahos_0377"/>
    <dbReference type="GeneID" id="10599818"/>
    <dbReference type="KEGG" id="aho:Ahos_0377"/>
    <dbReference type="eggNOG" id="arCOG04194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_2_0_2"/>
    <dbReference type="OrthoDB" id="75992at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405:SF3">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS2 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143430">
        <property type="entry name" value="TTP0101/SSO1404-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="11405" checksum="8771C1B0A5A47C8C" modified="2011-06-28" version="1">MLFLVFYDITDNELRNRVSSFLKQKGLERVQLSVFLGDLNSSRLRDVEAGLRMIRKRKGEGRFFILIVPITENQFKQRIIISDEKEDEEKEEGIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S8</accession>
    <name>F4B5S8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">IS607 family transposase protein TnpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0405</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775209.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S8"/>
    <dbReference type="STRING" id="933801.Ahos_0405"/>
    <dbReference type="GeneID" id="10599845"/>
    <dbReference type="KEGG" id="aho:Ahos_0405"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OrthoDB" id="41819at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="5">
        <location>
            <begin position="194"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="6">
        <location>
            <begin position="315"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="429" mass="50644" checksum="92D2C79183BD26AD" modified="2011-06-28" version="1">MSKNLRLNDFQPQEEYVYLTYSIKNNKKEETKILLENYRVLLQKALDYLWEKTKIERKEVKKGKKAFTKVKVKLPKKKEVYKALRDELEKINVLASHYVDKAISDAYSILGSWRRRAEEGQASLSKPRLKKVYVRVKSTLRKVEGESVRITVRPHEYVTFSWSHKWFSRRVEGLELGEPIIKEDKVHLPFRYKLPWSTPLDFLSIDSNLYTLDAYDGEKFITFSIKELYSLKFGMELKRGRIQSFASKHGRKGKELMRKYSHRERNRVLDYVHKFVNKLLEIYPVTIFAVEKLNKQSMFKDANDKLSKKVSRTVWRTIHRVLKYKAPLYGSFLKEVNPHLTSKSCPRCGWVSRKVGRTFHCERCGFTLDRQLNASLNIYLKMCGFPHIRDIPRMWVGVIPLKGRRGNGFPRDSGEAQGLRIGYNFMKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V2</accession>
    <name>F4B5V2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775233.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5V2"/>
    <dbReference type="STRING" id="933801.Ahos_0429"/>
    <dbReference type="GeneID" id="10599869"/>
    <dbReference type="KEGG" id="aho:Ahos_0429"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="18924" checksum="54950300DEF24E17" modified="2011-06-28" version="1">MENRMPLKEVIRKLEEKGVDVTEALLDILSREDPEDSSKERISLAEKYMQESKEYAEKGDVVQASEKAYKVAEEVVKALAEKFKTEEYEEFLKEGRWYTYLLGKASKTLSKKLGYWVLDGWNAGYDLHVWVFHERKYSVEDVKVSLKRIEEMLIEAKKLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Y3</accession>
    <name>F4B5Y3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1601</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Y3"/>
    <dbReference type="KEGG" id="aho:Ahos_1601"/>
    <dbReference type="HOGENOM" id="CLU_2067654_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="12802" checksum="793799C452CDFE61" modified="2011-06-28" version="1">MAFPTRAGGALSTSLSSLSNSSLVQYGQPHRDPLQLVHDPQRETLNPHRNLYLEYLGEVLLSSSRALLSISAVSFIARPPQLPQGGAQSPRDWINISRYGLALVSFSCTDVGSIKPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Z7</accession>
    <name>F4B5Z7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776410.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Z7"/>
    <dbReference type="GeneID" id="10601117"/>
    <dbReference type="KEGG" id="aho:Ahos_1617"/>
    <dbReference type="HOGENOM" id="CLU_1192572_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="26757" checksum="6CD33D16CA5171D9" modified="2011-06-28" version="1">MPECQCSYCIDYNDYLKCPAKHSQSLLNSIKSLLHSINIDYDIKSFKVVDRNTIANNIKLDGERFLGIYDGKDIIICPYAEDNGIHVRALLHESLHSIFPQEFENSTLDNIFRLIYEGAIDALSLFALCRDEQGKKEFEYFVERFESSAKSVIYINIAKIWATAEIQTIINKLKNFNNFRNEVEKEFNSNKMTYECYPGTEDCYYSSVAILAKILNTDESIISLSARLLFCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Z9</accession>
    <name>F4B5Z9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776412.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Z9"/>
    <dbReference type="GeneID" id="10601119"/>
    <dbReference type="KEGG" id="aho:Ahos_1619"/>
    <dbReference type="eggNOG" id="arCOG07288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157023_0_0_2"/>
    <dbReference type="OrthoDB" id="44045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14885" checksum="D5AD44D551457269" modified="2011-06-28" version="1">MSEVLNYALQEIQEGKYKIETDEENLELILHPVLLKVFKKDDKYSFVVQNIISVNTDKPRFGPLCSSNTLNSRPAKIRRTEVLKEPKIVLKMDEKIFNIIINVTNISIYPDYRDSFGSPCVMVSTVVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B607</accession>
    <name>F4B607_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Manganese transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1627</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B607"/>
    <dbReference type="STRING" id="933801.Ahos_1627"/>
    <dbReference type="KEGG" id="aho:Ahos_1627"/>
    <dbReference type="eggNOG" id="arCOG04531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020088_6_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF33">
        <property type="entry name" value="NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="405" mass="44190" checksum="872FB659E7B3703C" modified="2011-06-28" version="1">MITMRRNSLTMRRNELKFIKFFGPAWLVMMADMDASSIIGAAQTGALFKYGFIWIMLLLVIPLYVVQEVSGRIGIATGKGLGEVIRENYSKKLAILMALPMALTDAVTYAIEYLGIGIGLEVIGIPLILSIPLIYVVHIMIVTKRKYIQAERFLLLISAFLIISLFATLFLRGIKPYSPFYFSPSPQFLFLMAATVGAVVMPFMLFFQASATAIKLKEIGHCKCKDTAIKHMRKETLLGAVFTEILMVVVEMAFAGINVDPSTFASPQELSSVLTPLAGSLSPVIFGLGLISAGFLALVVISLGSAWGVAEALNVKNVNIIYIAESVPAVVLTLLVPSVNLVCIVLDLLVFFVYALIGPIIILGIISRNKKIMGEYASRGLSYFAYWISSIILLIIAIIASLSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B610</accession>
    <name>F4B610_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776423.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B610"/>
    <dbReference type="STRING" id="933801.Ahos_1630"/>
    <dbReference type="GeneID" id="10601130"/>
    <dbReference type="KEGG" id="aho:Ahos_1630"/>
    <dbReference type="eggNOG" id="arCOG11038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445245_0_0_2"/>
    <dbReference type="OrthoDB" id="387168at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="613" mass="67976" checksum="7026093B7945C4D8" modified="2011-06-28" version="1">MSIPKLIVLGVFLLLLVLSIIPSHSLGLTPLPITIKKASFKGNIADPYGFIEYKGNLLELFFNFSLTAYNLTSLTNITNYLSNATFRIYTTYDGKVFYLTNETNVTSISTALYENEVLAVWSYKSSVEYSLFNGTAWSSPRTLIAGNILSVKTNGSEILVLCKIFDEYHLLVFRNFSLVDNISLPVTVNYIVTFNSKIIIVSNVSISFSPSLHYPIGYSIGEVYGLYINGSKIFKLKGLGLPVYDNYSEFVLINFTKYNETVVSIYNICGKQLYNLILNEPLLGVFVQNNIVSISHVSAQGTTICMYYLIDGKAIEYNKIEIPSLFKVYSFSESNLVGYVNSTLILENITITSNISKSGICLNITFTPSLMKVYTPSLPCKPEVQIEETEYPGMTVLLINYTEPNYYSHYVSCVEILINGKIVCKISSSSATIPFYIYQNGTYNITVLAVNFLGSEKTCIITTTQVKPNVTVIPKPEIITTMESYPGYTLLNISYDARNVQDLICVQLFINGSLVKKFTSTSGYYIYNITSNGTYLITVKAISKLGSSINSTVESVKVMPLITTSTTTTTSSTMTKISTTSTAPPTSNLTIYLGIIGLIIVIALVSLIIFRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B626</accession>
    <name>F4B626_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1646</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B626"/>
    <dbReference type="STRING" id="933801.Ahos_1646"/>
    <dbReference type="KEGG" id="aho:Ahos_1646"/>
    <dbReference type="eggNOG" id="arCOG02693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43045:SF1">
        <property type="entry name" value="SHIKIMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="18"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="451" mass="48678" checksum="129B6F36CF05D857" modified="2011-06-28" version="1">MLKNSHPYFLLMSSISRVALLGLIGTIVEWYDYYLFIYAALLAFPTLFFPSKSYLISMLASVSSYAIAFFARPLGATVFGHIGDRLGRRHALSFDLVLVGIAMVSVGLMPTYATIGIIAPVAIFAFRFLQGLGVGGEWGGVATWVSEHATSKRALITSLIEIASPIGFIGAATVLLAFSKDFVTIGWRIGFLVGGSVAFLGAFLRYLATESLPFEKIKSEGKISKVPSLEVFKYAWKPIILLMLAIGGVFMAVYIPATVMPSLYLKVVGKSLGYPAYINFLGLQMPITSALIYVFAIAGLISTPIFGYLGDKLGRKASLILGDILIAAFAYPYVYGFLSGNLGLLFIMQFLIGFAAYGPYASMAAFYPESFPTKYRYSGASYGMQLAAAVEGGLMPILLVGLLGLPSQYLANSWIVTTFLALWGIISALATLGLKETKGTQLVEEKATITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B634</accession>
    <name>F4B634_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rieske iron-sulfur protein SoxL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1654</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776447.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B634"/>
    <dbReference type="STRING" id="933801.Ahos_1654"/>
    <dbReference type="GeneID" id="10601154"/>
    <dbReference type="KEGG" id="aho:Ahos_1654"/>
    <dbReference type="eggNOG" id="arCOG01720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074282_0_0_2"/>
    <dbReference type="OrthoDB" id="5623at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03475">
        <property type="entry name" value="Rieske_SoxF_SoxL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017586">
        <property type="entry name" value="Rieske_Fe-S_SoxL2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03171">
        <property type="entry name" value="soxL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="8">
        <location>
            <begin position="148"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="291"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="325" mass="34782" checksum="10E8AE599D8614DB" modified="2011-06-28" version="1">MIKFKLNKDEKPILSPSDFYFINKLLAKMRNPKTKFDSREFVKKGNDYLFNYVDKNLGGIDEGRRRFLKGLVIGIGAAAVVGLIPGLRVLQPPEVAAISGFPKSLLVDSSGSPILASKIPVNSPIITIYEYPLTGEPNFLLNLGDSSGKPVKLPPATVVVPQTGDKYTWPGGVGPYGSIVSYSAICQHLGCQPPYIHFYPPNHVNSAQESAPEPDTLTPQAVAAAQSNHVPAIFHCDCHGSTYDPYHGAAVLTGPTVRPLPATILEWDSSTDYLYAVGSIGVGIYPTGSNGVPSKDPKSDLESSFGSSVGTKTTVKETENPFSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B669</accession>
    <name>F4B669_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775273.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B669"/>
    <dbReference type="GeneID" id="10599909"/>
    <dbReference type="KEGG" id="aho:Ahos_0469"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="OrthoDB" id="41609at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="168" mass="19435" checksum="4F9C06D41C8B11EB" modified="2011-06-28" version="1">MYEILDYKTDPKSYIYVKVMEGLMESKLALEMLRKGMITNASSKAFIAVKAIISALVVKNFDNILKSKSEKEKDWYERVGYSAPTTGLIGISYDLAKLGYDVQLIVKTALLLHSFSYNGFDPNFVNYKDKDEVEKDIIMVIEFIKNKVKKFFEDIWDEKLESRLKEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H1</accession>
    <name>F4B6H1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase GroES domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1714</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776506.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H1"/>
    <dbReference type="STRING" id="933801.Ahos_1714"/>
    <dbReference type="GeneID" id="10601214"/>
    <dbReference type="KEGG" id="aho:Ahos_1714"/>
    <dbReference type="eggNOG" id="arCOG01458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_4_2"/>
    <dbReference type="OrthoDB" id="8709at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08264">
        <property type="entry name" value="Zn_ADH_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="154"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="331" mass="36007" checksum="DC3B52CB6533B2E6" modified="2011-06-28" version="1">MRALQFDKSGIENLKLVELPNPEVGAHDVRIKVKFAGVNPIDYFVVNYLPVKPMPHIPGAEVYGEIESVGDHVKSLKPGDRVIVYNRLFDGTCDMCLSSMEMLCRNGGIMSVITNGGYAEYLTVPEKNVFKVPDNMSEELASSLPVAGLTAYHALKEAGVNSNDTVVVFGASGNTGQFAVQFAKKMGARVIAVSKKVWLKDFGADYVTTYNKVVEDVSRVTGGKMASVVINSVGSSVWDLSLSSLGVNGRLVFFGGITGSEVKLDLSQIYGKHVKIIGTTGGRRIELMEILESCNDCKVKVWKTFSLEEGKDALKALFDPTRDGRILLKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I0</accession>
    <name>F4B6I0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6I0"/>
    <dbReference type="KEGG" id="aho:Ahos_1723"/>
    <dbReference type="eggNOG" id="arCOG06006">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091164_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="12547" checksum="8A7407E1427B89ED" modified="2011-06-28" version="1">MATVTLVLLTLGISLLTLTGSELWFLVFSSFIQLFLSIPIGWQVNLTDSVNFSQIIPTSEAFVCITLTPSAKTENSNAKVIFYAYAIAVSLIAISTLFVVPKAIYYASSISAFSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6I5</accession>
    <name>F4B6I5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776520.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6I5"/>
    <dbReference type="GeneID" id="10601228"/>
    <dbReference type="KEGG" id="aho:Ahos_1728"/>
    <dbReference type="eggNOG" id="arCOG02217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175270_2_0_2"/>
    <dbReference type="OrthoDB" id="42667at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039709">
        <property type="entry name" value="VapB3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244:SF2">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="74" mass="8838" checksum="E8563930197097EB" modified="2011-06-28" version="1">MSSVISVRIKKEIKEELEKQGIDIDQEVRKFLEELYIKVKAKEYVNKWIEELKDVKPSEEGFSIKSVREDRESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M4</accession>
    <name>F4B6M4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2250 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775350.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M4"/>
    <dbReference type="STRING" id="933801.Ahos_0546"/>
    <dbReference type="GeneID" id="10599989"/>
    <dbReference type="KEGG" id="aho:Ahos_0546"/>
    <dbReference type="eggNOG" id="arCOG05388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140235_0_0_2"/>
    <dbReference type="OrthoDB" id="55886at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15148" checksum="138B108CCB025BF4" modified="2011-06-28" version="1">MSELNDLMKKILIHIYKYGPDNPWYMARRLLGESGWAPKYNEDEIDAACRKLEEMGYLVRFQGALKKSVTSSVKPWLKVKAKELGHKPKGIYFDLSKEGRRIASDLYKEYKRSLESASGNKSNQKTNKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M5</accession>
    <name>F4B6M5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron (Metal) dependent repressor, DtxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0547</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M5"/>
    <dbReference type="STRING" id="933801.Ahos_0547"/>
    <dbReference type="KEGG" id="aho:Ahos_0547"/>
    <dbReference type="eggNOG" id="arCOG02099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="23919" checksum="15A21277090CB057" modified="2011-06-28" version="1">MELSEALENYIKEIYELEEVKGQAKVTDLILAFQVSPGTISKALDRLESLGLITRSNRKLKLTDDGRKIAERLIRSHRLSERLLTDVIGVDWIRAHEIAHRLEHIWPDDVLDKIDAILGFPSTCPHGHPIPGRMKIPTGKLLSEVNEGEYKVVMIVKEEEWILRNMEMLGIKPGETIKLLKKDEDKFIVQINGKINEITKTLADQVVVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N5</accession>
    <name>F4B6N5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775361.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6N5"/>
    <dbReference type="STRING" id="933801.Ahos_0557"/>
    <dbReference type="GeneID" id="10600000"/>
    <dbReference type="KEGG" id="aho:Ahos_0557"/>
    <dbReference type="eggNOG" id="arCOG00035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="OrthoDB" id="372052at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="222" mass="25861" checksum="BD0602E5BEB42542" modified="2011-06-28" version="1">MKVCVLYSGGKDSTYALHWAVFKGFEVISLLTLLPKREDSWMFQYPNVEFTKYQAEALGMNIIQMPTYGEKNNELEDLYVAFKKCKDLGAQGIVTGALLSDYQRLNANIIAEKLSLKTYSPLWRKDQEKYMYELIDEGFEFIITSTSAYGFPHELLGKVIRREDVDIIIRRAREYGFNPAFEGGEAETYVIYAPLFKRRLKVFGEIKRLGEDNWRFLIKRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P0</accession>
    <name>F4B6P0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Terminal oxidase, small hydrophobic subunit (DoxE)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775366.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6P0"/>
    <dbReference type="STRING" id="933801.Ahos_0562"/>
    <dbReference type="GeneID" id="10600005"/>
    <dbReference type="KEGG" id="aho:Ahos_0562"/>
    <dbReference type="eggNOG" id="arCOG05892">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2893232_0_0_2"/>
    <dbReference type="OrthoDB" id="36888at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="64" mass="7214" checksum="8198E979ABA06CE9" modified="2011-06-28" version="1">MVSFFKVLGVGYILAIALLVWELADATLHAAAAPYTMLFTIMAFIGFISFFLFVYFAPQEEEKK</sequence>
</entry>
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    <accession>F4B6P5</accession>
    <name>F4B6P5_ACIHW</name>
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        <submittedName>
            <fullName evidence="6">Fmu (Sun) domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0567</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6P5"/>
    <dbReference type="STRING" id="933801.Ahos_0567"/>
    <dbReference type="KEGG" id="aho:Ahos_0567"/>
    <dbReference type="eggNOG" id="arCOG00975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_7_6_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF70">
        <property type="entry name" value="TRNA_RRNA CYTOSINE-C5-METHYLASE, NOL1_NOP2_SUN FAMILY, FUSED TO N-TERMINAL NUSB REGULATOR DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="5">
        <location>
            <begin position="94"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="358" mass="41504" checksum="B4ACD2A861B16513" modified="2011-06-28" version="1">MRKLIKDFLTTLLYFVEEKGYPLPVAFKKTKDIKKVKGMNYDELYEISRLLLLSYNSLKGKRSKKVDQFLQGNYEILLPLWAREELSKYLDVKPLENSFRIKNTWVRINTLKADVDKVLKSLENQGVNFEVDKDVYYLIKVKNEVQLKKTKEFMNFEVIIQDKASVLTVESLEVEKNDKIIDLSSAPGTKASQIMQLGENTVELFIADVDINRLKREVDLLKKMGVDMNKVHIIHQDSTSNSMLRSDKVLLDAPCSSSGMISNEPAIMVNLTREKVMYYSQLQRKMINEARKIIDADYIIYSVCSLFPEEGEEHFLNLKTEKPKIVGERPYIDNVNGIRLFPHINLTEGFFITKILLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Q0</accession>
    <name>F4B6Q0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">thymine-DNA glycosylase</fullName>
            <ecNumber evidence="12">3.2.2.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_0572</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.29" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775376.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Q0"/>
    <dbReference type="STRING" id="933801.Ahos_0572"/>
    <dbReference type="GeneID" id="10600016"/>
    <dbReference type="KEGG" id="aho:Ahos_0572"/>
    <dbReference type="eggNOG" id="arCOG00459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_4_2"/>
    <dbReference type="OrthoDB" id="19248at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF18">
        <property type="entry name" value="ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="44"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-hairpin-helix DNA-binding motif class 1" evidence="13">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE93460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="26628" checksum="527A109499DB9678" modified="2011-06-28" version="1">MKCTGKEIIERLRKTYKRDPKEYVAYDVWINFKDPFKVLIATLLSQNSTDKGTYKAFYTLEEKIGVTPDNLIKSSLEDIASCIRNIGIYRIKAERIKELAKIIKEKYNGDLNKILDKEPKEAREELLSLPGIGEKTADVVLLTCKGYPYFPVDTHIKRISQRLGIASGSYEQISASLMRLFDPKDYLEAHHLLIAHGRNVCKAKNPLCEKCILNDCCEYYSRINKNKPDNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R4</accession>
    <name>F4B6R4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="11">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="11">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pheS</name>
        <name evidence="13" type="ordered locus">Ahos_0586</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775390.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R4"/>
    <dbReference type="STRING" id="933801.Ahos_0586"/>
    <dbReference type="GeneID" id="10600030"/>
    <dbReference type="KEGG" id="aho:Ahos_0586"/>
    <dbReference type="eggNOG" id="arCOG00410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="OrthoDB" id="372178at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="226"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="351"/>
            <end position="353"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="468" mass="53974" checksum="08D228CFF926D3AF" modified="2011-06-28" version="1">MLSENEIKIIDFLKKKENKSAYSTELSSVIPETAVFSLAYLLKDKGYVSVKEEEKENIILTKEGEERLKEGLPEDNLINFLKGESKKIQEVAKTFKDFNIALSWARKKGLIKVDKGEIIPLKSSYVSPEYVVLKKISSSQDLSEDDKKYLKELESRGLIEIKKNKVLLLTLLKEPEIRPTITYLTPELLRNKEWTKYELKEYNVEALPPFIPLGKKHYFKEFLEHVKDVMVSLGFKEVKSDYVEMEFYNFDMLFQAQDHPAREIHDSFKVNGKGELLNQNLVKRVKEVHEKWWKYSWNQEVAKSLVLRSQTTATTARVLSTSSNKEIKVFTIGKVFRPDAIDATHLIEFHQLDGLVIEENFNFRELLSTLRAIFSGIGIKEVKFKPAYFPFTEPSVEVYGYIQGLGWVEMAGAGLLRPEVTEPAGVTMPAGAWGLGLDRLAMLFLGIKDIRNLYSDDIEYLRNRKVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U5</accession>
    <name>F4B6U5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776553.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U5"/>
    <dbReference type="STRING" id="933801.Ahos_1761"/>
    <dbReference type="GeneID" id="58788763"/>
    <dbReference type="KEGG" id="aho:Ahos_1761"/>
    <dbReference type="HOGENOM" id="CLU_3094296_0_0_2"/>
    <dbReference type="OrthoDB" id="41276at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="51" mass="6018" checksum="E1256ECA248AE437" modified="2011-06-28" version="1">MIAIIYSCIGPLYIKIAEEKCENIEEIKSKWKYACLIEVFDDKKEKLLYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y3</accession>
    <name>F4B6Y3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1801</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776591.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y3"/>
    <dbReference type="STRING" id="933801.Ahos_1801"/>
    <dbReference type="GeneID" id="10601301"/>
    <dbReference type="KEGG" id="aho:Ahos_1801"/>
    <dbReference type="eggNOG" id="arCOG01681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_5_3_2"/>
    <dbReference type="OrthoDB" id="57427at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033788">
        <property type="entry name" value="HTH_metalloreg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF36">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="115" mass="13180" checksum="3B1753E499DC4164" modified="2011-06-28" version="1">MINNNESLLLELESFFSALSDRTRLEIVFYLLEKGKATVQDMSRDLKKSQSLISHHLSCLRNCGVVKVEKNGKYSEYSINGEDVKEIILIATRHAKNYGKSILSCDIIKEEKENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B743</accession>
    <name>F4B743_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="11">G1P dehydrogenase</shortName>
            <shortName evidence="11">G1PDH</shortName>
            <ecNumber evidence="11">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">egsA</name>
        <name evidence="15" type="ordered locus">Ahos_0639</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NADP(+) = dihydroxyacetone phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775442.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B743"/>
    <dbReference type="STRING" id="933801.Ahos_0639"/>
    <dbReference type="GeneID" id="10600087"/>
    <dbReference type="KEGG" id="aho:Ahos_0639"/>
    <dbReference type="eggNOG" id="arCOG00982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="OrthoDB" id="8656at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08173">
        <property type="entry name" value="Gro1PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002022">
        <property type="entry name" value="PRK00843.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616:SF5">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="93"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="115"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE93526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="347" mass="37587" checksum="897EC3455F47E5C2" modified="2011-06-28" version="1">MEMREHIISLPERIFIGNGIISRIDEYFSELGLTGPFLIVTGSNVRRLIVSKILDKIPADVIEVQEANLDEVRKVEEFAKKHESNVILGVGGGKAIDVAKFSAFELNKRFVSIPTAPSHDGITSPFAAIKGLGKPISMKAKEPTAIIADIDVLSSAPRRLINAGIGDTVGKIVAVRDWRLAAKLRGEYYGDYTASLALLSARHALNCTKIINKDLKYGVRLLVEALISSGVAMGMAGSTRPASGSEHLFAHAVEMLYPEGPLHGELVGVGTIMMAYIHGIKWKEIRKALIRIGAPVTAKQLGVPDEIIIKALTIAHTIRPERYTILGDRGLTWASAEKVARNTGVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B776</accession>
    <name>F4B776_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS607 transposase protein TnpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_1821"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_17_0_2"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="359" mass="42224" checksum="8DA00C65D15064BB" modified="2011-06-28" version="1">MKLRVKVDYKTYQKLKEVEEEYREVLEEAINYGLVNKTTSFTRIKSGVYKTEREKHKNLPSHYIYTACEDASERLDSFEKLKKRGRSYTEKPSVRRVTIHLDDHLWKFNLEKISIAVKRSRVFISPIFPKIFWRYYNTGWRIASEARFRLLKGNVVEFYVIFKRDEPKPYKPKAFIPVDLNENSVSVLINNKPVLLETNTKKITLGYEYRRKLTTTGRSTKDREVRRKLKRLRERDKKVDIRRKLAKLIVKEAFESRSAIILEDLPRRTPELMIKDVKDSQLRLRIYRAAFSSMKNAIIEKAKEFDVPVVLVNPSRTSTVCPIHGTKIVYQPDGGNAQGLVFVRRGKRGGIGTWLRCTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B785</accession>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1830</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776620.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B785"/>
    <dbReference type="GeneID" id="10601330"/>
    <dbReference type="KEGG" id="aho:Ahos_1830"/>
    <dbReference type="HOGENOM" id="CLU_1458126_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="185" mass="21712" checksum="1AE0C7431CBA0FF0" modified="2011-06-28" version="1">MLVDELQKKGALPDDIKMIKIDEKYLIEYLSTLKAEEQCKEIIGEVIRSIKEGNYAKAYFLLMILQRALQDVMKNKVINGLTDITSIINKREINKLANKMCKKMDQEDILKYSKAIEDLKSRDLWNYVYDQVLKALPNENVYKKMLKEIRRISNMIVHELYLPHKIELEDIFLLSFVVNLLKGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7E5</accession>
    <name>F4B7E5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0273 protein Ahos_1892</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1892</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0273 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776680.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7E5"/>
    <dbReference type="STRING" id="933801.Ahos_1892"/>
    <dbReference type="GeneID" id="10601391"/>
    <dbReference type="KEGG" id="aho:Ahos_1892"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="5">
        <location>
            <begin position="4"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="257"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="287" mass="32148" checksum="4FCECFD63FCAD786" modified="2011-06-28" version="1">MNVRRVRTYIPGLDEILYGGIPERNIVLVSGGPGTGKSISAQQFIYNGLIRGEAGVYVALEEHPVSVLRSFHHFGWDVTKFEKEGKFAIVDAFTGGYGSTAQKERYVVKNVDDVKELADVLRQAIKDLKATRVAIDSVSTLYLTKPAMARGIVMTLKRIISGLGCTAFFVSQVSVGERGFGGPGVEHAVDGIIRMDLDEVEGKMYRSIIVWKMRDTKISMVRHPMEITDNGIVVEWDKYLKITNFSATVEPLPKEEVDEMRKALEEEEKAEKEKVTEEIEEVEEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G1</accession>
    <name>F4B7G1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF131 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775482.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G1"/>
    <dbReference type="STRING" id="933801.Ahos_0679"/>
    <dbReference type="GeneID" id="10600130"/>
    <dbReference type="KEGG" id="aho:Ahos_0679"/>
    <dbReference type="HOGENOM" id="CLU_149108_4_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00304">
        <property type="entry name" value="TIGR00304 family membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10237" checksum="0B25F4249E73E1B1" modified="2011-06-28" version="1">MKLSVIGVGMSLIFIGFALLFISALSCTVSTPSTTSTAVGGLILIGPFPIFFGVGPKNELLPLTIFGIIFTIIAIIFFILTFYMFKKWSQRPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M0</accession>
    <name>F4B7M0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776682.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M0"/>
    <dbReference type="GeneID" id="10601393"/>
    <dbReference type="KEGG" id="aho:Ahos_1894"/>
    <dbReference type="eggNOG" id="arCOG03286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010399_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053494">
        <property type="entry name" value="GH15_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045582">
        <property type="entry name" value="Trehalase-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041084">
        <property type="entry name" value="trehalase_H1_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF0">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19291">
        <property type="entry name" value="TREH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trehalase-like N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="2">
        <location>
            <begin position="216"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="584" mass="66325" checksum="38DC6B80479EF4D9" modified="2011-06-28" version="1">MKPLPCINNEFTGALIKGTDIVWLAFPRYDSPSVFSSILDPQRGGHFLISGEVAKQEYLVPNVLLTTLKDGSEITDLLLHGEHGLVRKIVAKNPLKVSILPVFNYGKSPATIERLSDKVFKFSNPESSEFLEVHLLFDKLDQISENSWTVYGEGYIYMGYFKDDRFGIFGKRQLKFDVARGFERTIAYWRNTMKRGRSKGKIAEIEIEGYTGDQLLNAYQTSVGILLGLLYNPTGAIVAAPTTSLPEVEGGSRNWDYRFAWVRDSSIVAEALISAGFSAEARRIIEFFSRMVSFTSKPFIYPLYSIDGSVPPKEEEIPWLSGYENSKPVRIGNAAAGQLQLDLEGFFLDAFFKYYEATGDENYIRSHVDVIEYIADWVSENWKLEDVGIWEERGVQGHYIHSKVMMWVALDRAGKLMNAIGKENPWKETRHEIKEWVLQNGVSNGIFVKKVGSNEVDAALLTLPIYGFIDVQDETFLNTLREIENQLLYKGQLKRYKRDFLGEAKYPFVLASLWLARVYIRLGRIQEAKNIIKGILDATQGIYLVGEHIDPDRKEFTGNYPQAFAQANLILALDELAEKKEDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M3</accession>
    <name>F4B7M3_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776685.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M3"/>
    <dbReference type="GeneID" id="58788767"/>
    <dbReference type="KEGG" id="aho:Ahos_1897"/>
    <dbReference type="HOGENOM" id="CLU_3178656_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="46" mass="5300" checksum="66310A4BAF772101" modified="2011-06-28" version="1">MRSGKDGEYYFVTIESDEELEKLKELTLKKIRAKNGKLSYASQSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T0</accession>
    <name>F4B7T0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Drug resistance transporter, EmrB/QacA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1954</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776742.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T0"/>
    <dbReference type="STRING" id="933801.Ahos_1954"/>
    <dbReference type="GeneID" id="10601453"/>
    <dbReference type="KEGG" id="aho:Ahos_1954"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000503">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000021">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="7"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="468" mass="50323" checksum="F9DE811E893B4746" modified="2011-06-28" version="1">MDRVSIITFVVVLGTMMAAIDSTIVILALPVMLQCLHTDLLTLIWVILVYLLTTAVLTTQLGRLGDRFGRGKIYNLGFLIFTVGSAFCGVSPNASFLISSRTIQAVGASMLQANSGAIIADNYPPNRRGKAYGYTSIGWNVGAILGIILGGILTTFVGWRYIFYINVPIGIIAIILGLREIRDSRIVHAKFDIFGVLILASSLILITYGAAEVAGVGITPFNLFLIITGILLLFPFYLIEKRQPFPVIDFKVFRNRVLTASLLASFLQSAGYLATAFILIMYLQGIRGLTPFNASLLLVPGYVLASMVGPITGRLSDKIGARIPATIGIGLMIIAAFIYSNLTLTTPYYVIVVTSIIGGLGSSFFYPANNSAIMSNAPRSIYGGISGIMRTLANIGILISYVIAISIASLSIPRYVAFEVFLGTYSLGGLSSKFLIGIHSAFYASIVILSIALVLSAIRGKEERSYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T1</accession>
    <name>F4B7T1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FAD linked oxidase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776743.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T1"/>
    <dbReference type="STRING" id="933801.Ahos_1955"/>
    <dbReference type="GeneID" id="10601454"/>
    <dbReference type="KEGG" id="aho:Ahos_1955"/>
    <dbReference type="eggNOG" id="arCOG00337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_9_2_2"/>
    <dbReference type="OrthoDB" id="26910at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051914">
        <property type="entry name" value="FAD-linked_OxidoTrans_Type4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42934">
        <property type="entry name" value="GLYCOLATE OXIDASE SUBUNIT GLCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42934:SF2">
        <property type="entry name" value="GLYCOLATE OXIDASE SUBUNIT GLCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="20"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="436" mass="48706" checksum="058DC8F67D5052F5" modified="2011-06-28" version="1">MEELLDIEHSKEEREDFAGYKISPTMIFFPKNESEVVRIVKYAYTHNIPIVPWGSGSSLTGATACKDCILVDLSKMDKILEINDVDWYVRVQPGVKLIDLYEAVESKGFFFPPDPASFFLCTVGGAISEGSGGMRGVKYGPLREWVLSLRVVLPTGEVVKVGEPLRKNRAGYDLTHLFVGSEGTLGIITEAWLRIIPKPRKKIYTVKVLLPDFSTVAENIIGIRKARILPEFSEYIDADVIRALNRNLNAGLEESEGGMLLISIEEDQLQDLLKVLKGKVEVLEGDEAERLYSLRSQAAIAVKAETRTFYAEDIVVPISKLPEAISKLNEIAKKHNTKFYIISHIGDGNLHPNIVVDDEKEKEEIFNEIAKVAIDLGGSVTGEHGVGIQKAKMMSEQIKSHNGERVLDLMIGIKKMLDPKNIMNPDKYVELAYKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T4</accession>
    <name>F4B7T4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease NucS</fullName>
            <ecNumber evidence="6">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nucS</name>
        <name evidence="9" type="ordered locus">Ahos_1958</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776746.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T4"/>
    <dbReference type="STRING" id="933801.Ahos_1958"/>
    <dbReference type="GeneID" id="10601457"/>
    <dbReference type="KEGG" id="aho:Ahos_1958"/>
    <dbReference type="eggNOG" id="arCOG01304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069350_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22341">
        <property type="entry name" value="NucS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.180.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048301">
        <property type="entry name" value="NucS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048302">
        <property type="entry name" value="NucS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049173">
        <property type="entry name" value="NucS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003270">
        <property type="entry name" value="PRK04247.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814:SF1">
        <property type="entry name" value="ENDONUCLEASE NUCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21003">
        <property type="entry name" value="NucS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease NucS N-terminal PH-like" evidence="8">
        <location>
            <begin position="26"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease NucS C-terminal" evidence="7">
        <location>
            <begin position="128"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="241" mass="27720" checksum="3361B40E310FB32D" modified="2011-06-28" version="1">MMEKYKFFDLPEFSTVKDILENERKNYVITLFGLCKILYAGRASSIATYSSRLITFKPDGSVLVHSNKKLKPVNWQPSSSEVKVELGDELKIYVERKRPKEVLEILMPIVYYLSLSKVDEGEFHLFGSESEMVEEVVKNPELISRDFIPLEREYQTPFGKIDLLGVTSTGYLVLEFKRAQAGLEAVSQLKRYVDYLSWKGEKVRGGIVAPSISKSALKLLKKYELEYFQLSPRLLSTIRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U5</accession>
    <name>F4B7U5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1969</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U5"/>
    <dbReference type="STRING" id="933801.Ahos_1969"/>
    <dbReference type="KEGG" id="aho:Ahos_1969"/>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870602_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="136" mass="14391" checksum="37EB5AFDFEFB25DA" modified="2011-06-28" version="1">MSPLCGSPIGLGGRYATFPFQGFGLGGIIQRLLGFFGGMGFQTYPFMGQNLGVLQVIHPSTFSSYPNIAYTVRLCQSPGFLGVETGHAGLLSLPGMVDDYTLHNSLLSALGMTGELMPYAGMIPEDSLFSIIGSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V8</accession>
    <name>F4B7V8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="6">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpo5</name>
        <name evidence="6" type="synonym">rpoH</name>
        <name evidence="8" type="ordered locus">Ahos_0749</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775551.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V8"/>
    <dbReference type="STRING" id="933801.Ahos_0749"/>
    <dbReference type="GeneID" id="10600206"/>
    <dbReference type="KEGG" id="aho:Ahos_0749"/>
    <dbReference type="eggNOG" id="arCOG04258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058320_4_0_2"/>
    <dbReference type="OrthoDB" id="30537at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9253" checksum="79854EA411386C76" modified="2011-06-28" version="1">MRSSSNKKIDPRVHSLVPKHEVLSIEEAYKVLKALGVSPEQLPWIKASDPVVKAIGAKPGDIIKITRKSQTVGETVIYRYVISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y8</accession>
    <name>F4B7Y8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved archaeal protein, DUF99</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y8"/>
    <dbReference type="KEGG" id="aho:Ahos_0779"/>
    <dbReference type="eggNOG" id="arCOG00928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095956_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="Endo_dU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518:SF2">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="Endo_dU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="18234" checksum="CA82D2CB543B136B" modified="2011-06-28" version="1">MTYKGKRGKCPLVSVTFDGYKLVDVDVEFITVDGDDATTAYKNLRKGDIKILDSIIVGGFNYIIPDNNYIIYYASKPDIDSILNAARKHYNDKRVNVIKEFLSNMIALSTNRGTVYVNTDLDLKMVKSVIEYYQIFSKYPEPIKYAHIIGKAIGQSQLISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B824</accession>
    <name>F4B824_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776763.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B824"/>
    <dbReference type="STRING" id="933801.Ahos_1976"/>
    <dbReference type="GeneID" id="10601475"/>
    <dbReference type="KEGG" id="aho:Ahos_1976"/>
    <dbReference type="eggNOG" id="arCOG07301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_736971_0_0_2"/>
    <dbReference type="OrthoDB" id="42867at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="383" mass="44840" checksum="10055913B2E11D97" modified="2011-06-28" version="1">MLESILPNSILIDAGNRKEFTDPNFVVSLFLLPFMYEKKPEEKEYLEVIESIKFESYDELNEIRNKDFPMYATYFQQKVLNDYISGKAYARNAISFLLNKFKDEKEAEKIKEEYEDFILDKLSLKEFFKPSSDEEPKYVFPYVWRFYTPNFDETVKKVTSGKEIVSDYMGLTTYVILVSKELFPFFRPYVFLSNSPPRIGLKMGEVLIDPEEIIVTQLNEDRLSFSRKFLEKELGKLESKELSSFIESKSETSRRIILSSLRYANWALKKSGISLEDAVHLFVKLRELIDDVERDFQKAIDNGVDFKEIKEEVKKYGWLNLEVTPAVNPLALKKSIRIISIMKEIGDMYFIPYSIIMSAIVSTYVSGKDYKLLLYGLKTNKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B855</accession>
    <name>F4B855_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Precorrin-8X methylmutase CbiC/CobH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2007</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B855"/>
    <dbReference type="STRING" id="933801.Ahos_2007"/>
    <dbReference type="KEGG" id="aho:Ahos_2007"/>
    <dbReference type="eggNOG" id="arCOG02247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827999_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03415">
        <property type="entry name" value="CbiX_CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="entry name" value="Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004449">
        <property type="entry name" value="PRK05782.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="Precorrin-8X methylmutase CbiC/CobH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC" evidence="7">
        <location>
            <begin position="175"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="366" mass="41036" checksum="1FA8235A8DE808B6" modified="2011-06-28" version="1">MRLFATLTSFSVKRSKNNLYKSHIYILDKLSINVDTAIIIITHGSRRNTFVEDMEGVAKYIEDKLQIPVYLSHNEFTEPNWRNLVSSLLEKGINKFIFALAFLGRGNHVAKDIMGSFGVNEFYKWEEAQYEGRKVKVYFTRPLADSPLVKLSLLYRISSAVRKDNYFNFLEDPEEIEENSMELSRQKVREITGKDGEELEIISRAVYASGNLEIARYIYISKDAIEMGVSALKSGVGILTDVKMVKAGLRWNAENYLDDAVELAKKSKITRTAAGIRIGLSKEPKIVVIGNSPTALVEAIKMHEEEGVEIPLIVATPPGFTNAVEAKEKLISTDIPCIVLRGNYGGSNIAVSIMNEIIRYARGKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B878</accession>
    <name>F4B878_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776817.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B878"/>
    <dbReference type="GeneID" id="10601529"/>
    <dbReference type="KEGG" id="aho:Ahos_2030"/>
    <dbReference type="HOGENOM" id="CLU_2613393_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="8120" checksum="52297CE289411A03" modified="2011-06-28" version="1">MTLALGYSSAILIFTIGLIITGSSQISIPSPTPSSILFAVYFTLITAGSIAILTGIIGILDAIFILVYALTMKSLPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B888</accession>
    <name>F4B888_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome c oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776827.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B888"/>
    <dbReference type="STRING" id="933801.Ahos_2041"/>
    <dbReference type="GeneID" id="10601541"/>
    <dbReference type="KEGG" id="aho:Ahos_2041"/>
    <dbReference type="eggNOG" id="arCOG01238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_498421_0_0_2"/>
    <dbReference type="OrthoDB" id="28065at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00919">
        <property type="entry name" value="Heme_Cu_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="2">
        <location>
            <begin position="25"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="537" mass="60175" checksum="6F22D41BCB001694" modified="2011-06-28" version="1">MSSVSKGLINLVNAVFQLDKDWVSRITMAMIVLSLVWGILGIIDALMVRIQELSWGVSQTLVLTSQEYYAGIGLHAMRDLFGFAVQLEIAIFVFLSYKILHFQPRAKWFLNIGFILFNIAFMLLEGPIVAYPTFNDNYFPAESWYYLSPYGLPAYSQDVVSPLWFFGWELMDIGVYIYVIWIIYHYYLATKTYKEKLPIFGVFALMTSLMIAIGWSGEVAANTWDILNYYGLVGLNVISNQIAFGILWHSIVYISWMPAIGAMYYLIPLLAGRPVYSDRMGRVAALLYLVFSNNVPIHHLYMVDLPVYLKILQEVLTFGVVVPSMMTFFNLWATVKGAQVKMNLITAWISISFAGAIAAGVTGIANGDISFDAIIHDSMWVVGHFHAMIFWSIVPAGFATLYYMVPLLTGRMWYSNKLGWIHLVGYMIGTAMIIVGFDALGLAGLVRKAEIYPLISAYVTPEIIASLGAMIADTSTLLWLGNLVLTLLKGKTQNFEGVSLDNAVSFIASTLEAPRLDKIEVPLHNVKSALIRAFEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B893</accession>
    <name>F4B893_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="40580at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14198" checksum="744E06D6FCCEF0AA" modified="2011-06-28" version="1">MKTVGLIAFIVVLAIELIFMNALNSLNQGISQLSNINIFSQPKMSFFAEGNKLEVCINNPENFPVVVCSINGKEISLPSSAVIPAQTAENITLQVNNYHEFYSNIKNGDCTVFIKLHFLNVTVCTSETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B897</accession>
    <name>F4B897_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycine transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ordered locus">Ahos_2050</name>
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    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776836.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B897"/>
    <dbReference type="GeneID" id="10601550"/>
    <dbReference type="KEGG" id="aho:Ahos_2050"/>
    <dbReference type="eggNOG" id="arCOG04641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005115">
        <property type="entry name" value="Gly_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30506">
        <property type="entry name" value="INNER MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30506:SF3">
        <property type="entry name" value="UPF0126 INNER MEMBRANE PROTEIN YADS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03458">
        <property type="entry name" value="Gly_transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine transporter" evidence="7">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine transporter" evidence="7">
        <location>
            <begin position="93"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="201" mass="21324" checksum="ABCB6BC1F8D8CC76" modified="2011-06-28" version="1">MSLILLITNYIGIIAFSISGSMKAIEKKMDLLGVITLGFSTSLAGGILADLLLGITPPTNLIYIPYPTASFIASIVTFAFYKKFTHVSKPLIYADALGLGAFTASGASLAFSLRPDPLLVIMVGTITAVGGGVLRDILANEIPLVLTKEFYASASIIGSTIYYGLAELGVQTGFLTTLVLLLTFALRLLAIRMKWKLPNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A3</accession>
    <name>F4B8A3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo4</name>
        <name evidence="1" type="synonym">rpoF</name>
        <name evidence="2" type="ordered locus">Ahos_0816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775618.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A3"/>
    <dbReference type="STRING" id="933801.Ahos_0816"/>
    <dbReference type="GeneID" id="10600283"/>
    <dbReference type="KEGG" id="aho:Ahos_0816"/>
    <dbReference type="eggNOG" id="arCOG01016">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00864">
        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HRDC_dom_sf"/>
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        <property type="entry name" value="Rpb4/RPC9"/>
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    <dbReference type="InterPro" id="IPR010924">
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    </dbReference>
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        <property type="entry name" value="PRK14981.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT RPO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="RNA POLYMERASE RPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_F_arch"/>
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    </dbReference>
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        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="113" mass="12993" checksum="CD10CA514E19BFDD" modified="2011-06-28" version="1">MSSVIIEEEHYIPYSVAKKYVYELIRNGNNSSILQRTYEYLNSITKCNDEDANKLMEELAQIVEKEEIRAVIASICPTTVDEVRSILASDSKNYGQEILEKIVELVKKYLQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D8</accession>
    <name>F4B8D8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl29</name>
        <name evidence="5" type="ordered locus">Ahos_0851</name>
    </gene>
    <organism evidence="5 6">
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        <lineage>
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            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B8D8"/>
    <dbReference type="STRING" id="933801.Ahos_0851"/>
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    <dbReference type="KEGG" id="aho:Ahos_0851"/>
    <dbReference type="eggNOG" id="arCOG00785">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_158491_2_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Ribosomal_L29_HIP"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal_protein_uL29"/>
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        <property type="entry name" value="Ribosomal_uL29_CS"/>
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        <property type="entry name" value="Ribosomal_uL29_sf"/>
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        <property type="entry name" value="L29"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10916">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35/50S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10916:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29C"/>
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    <dbReference type="Pfam" id="PF00831">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="72" mass="8483" checksum="61FF0072AC132EF4" modified="2011-06-28" version="1">MPLDPEELRKMDIKDLYKKLDEYNAELLKYRAESRMGTLKNTSAIKNVRKDIARILTIISEKKRSKKNEKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G8</accession>
    <name>F4B8G8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G8"/>
    <dbReference type="STRING" id="933801.Ahos_0881"/>
    <dbReference type="GeneID" id="10600352"/>
    <dbReference type="KEGG" id="aho:Ahos_0881"/>
    <dbReference type="eggNOG" id="arCOG00286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_516412_0_0_2"/>
    <dbReference type="OrthoDB" id="10575at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053657">
        <property type="entry name" value="Ced-DNA_import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051162">
        <property type="entry name" value="T4SS_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041017">
        <property type="entry name" value="DNA_import_CedB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121:SF6">
        <property type="entry name" value="SLR6007 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30121">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJGR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12846">
        <property type="entry name" value="AAA_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="278"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="596" mass="67888" checksum="EA07658AB9B74890" modified="2011-06-28" version="1">MNEDKLSFVFISILIIVLTIVYRNPLLLLLLVIVSLVAFKHGKIDIGPLIQKIPFFNDSINLSSIKIEDGYIQKGNEYIGVLLIYDIPVDYKDLSENSLRNSIASFYKILQVGEQVDIFFRKKYVDSIKYKEVLLHKAQNLRIIIDSDPSNSKAKRELEIINFLLDRISEGEYPFKYEIYLLIHSKNKGRAIQLAEVISKGLEGLNIRSRLAKKDEISSLIFLSECKCNKISIPSQVPFLTPFSVDKLPKFQLRNDGILLGKDINHGIPVFWNIDTSENPHILIIGPTGAGKTEFLINTSLQLSLNYSIPIVFFDTKGDIKQRIIKRGLEAKILNPLYHGISLLKANNIPIQVRAMQIEKILTNSFDLDKIHSSIIFKLIYDTLEDYYSGKISYLNWDVIDEKAKSMLDEITYLYISKIIKIIKSLDHGGNIVDYIIPGLNVIDVTMIKSETLRKIIMYTVITDIYNKFSASVDNGTKIVLVLDEAWTVLKNEKVDYPLVADLVKRGRGHGIALLMATQNIEDLGEMSSVYLDNIGLLIFMNNGDKEFWNNVVRRFVNINDEEIYNNLTFLGRGEALVRFLGDPRPLLIKTDVITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H3</accession>
    <name>F4B8H3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein kinase catalytic domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2054</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776840.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H3"/>
    <dbReference type="STRING" id="933801.Ahos_2054"/>
    <dbReference type="GeneID" id="25395619"/>
    <dbReference type="KEGG" id="aho:Ahos_2054"/>
    <dbReference type="eggNOG" id="arCOG03682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_355138_0_0_2"/>
    <dbReference type="OrthoDB" id="41005at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="458"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="773" mass="86718" checksum="9129DC56479BB01F" modified="2011-06-28" version="1">MRVNEKIVSTIRLSFIASSLLVSFIIFYSMINDISLFLSNSIIFIVTESLITYALAMSIKPCLKSIGIAMVISSLLIPLFLPQTFSSMLSTISISTLLYITFIVLTSLLLLFALFFGGTKLQIIYLVITAFILLPILQYSFLNIRCFSCIFKYLPYSVLFYNHIFLFYTVTGILGFVLSASKKAERLVFNAVNNVALPYFLSALLPLIFLPLILNNVSTLNEILYYIINHFTTYLILVMIILAVFGSIIFRGQRDMELAIVVSLASLGLAWFSFIYSPYYTLVFLILGSSVIPISLSNPQLIENKLFSAINSNLYNRAEKYLDALLKLNYTYTKIFCDAASKFECNSISWIYSKFAGKIIYNNCNHLSNVVNCIISKNVIPSDASVLLDVLVLKDKNSAEKFASYLLTKDLPQPIKEKAKSTLASIIGITSNTVNPRQLPPLESWDPNIWIGSNLYGYKITKVLGSGGSSYVLVGERNDQKYAIKVAKISKSNVNDILNVFGNISKESSNLQSISEKSPNIVRIFGFFADINSIMDVVGGKSEVYYSMPPAIVMELMEGGTVEDLLKNQAVVLSSYWKKVVGLISLNIANALKVVHQEGYVHLDVKPSNIFFNKYPGKTAEEIYENIRNGVISVKLGDLGSARKIGERVLEYTPQYCSIDQVEAMLTNKGADVRMDVYALGATIYKMLTQTQFNPPKVAELMDKAVQSYVNNQPYAIYLEEARKELEAFHQRFSLPAEYAEFNPLIKIMTSPNILKRPNINYVIMELNKLILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I6</accession>
    <name>F4B8I6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucose 1-dehydrogenase</fullName>
            <shortName evidence="9">GDH</shortName>
            <shortName evidence="9">GlcDH</shortName>
            <ecNumber evidence="9">1.1.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gdh</name>
        <name evidence="12" type="ordered locus">Ahos_2067</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in the degradation of glucose through a non-phosphorylative variant of the Entner-Doudoroff pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose + NAD(+) = D-glucono-1,5-lactone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14293"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose + NADP(+) = D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14405"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the zinc-containing alcohol dehydrogenase family. Glucose 1-dehydrogenase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.47" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776853.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I6"/>
    <dbReference type="STRING" id="933801.Ahos_2067"/>
    <dbReference type="GeneID" id="10601567"/>
    <dbReference type="KEGG" id="aho:Ahos_2067"/>
    <dbReference type="eggNOG" id="arCOG01459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_1_0_2"/>
    <dbReference type="OrthoDB" id="41394at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047934">
        <property type="term" value="F:glucose 1-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047935">
        <property type="term" value="F:glucose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019595">
        <property type="term" value="P:non-phosphorylated glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08230">
        <property type="entry name" value="glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02127">
        <property type="entry name" value="Glucose_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026583">
        <property type="entry name" value="Glc_1-DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031640">
        <property type="entry name" value="Glu_dehyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050129">
        <property type="entry name" value="Zn_alcohol_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43401:SF2">
        <property type="entry name" value="L-THREONINE 3-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16912">
        <property type="entry name" value="Glu_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="10">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="147"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="191"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="274"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="359" mass="40069" checksum="5BE5711BDDA5D536" modified="2011-06-28" version="1">MKAIVVKPHQPGMEVKDVNIEEKVGKGQVLVKTLYTGVCGTDRGIVGNKLSFTRPQEGWSELILGHEAFGQVEEVGEGVKELRKGDYVVPVVRRGCGVCLNCKIGKQDFCETGNFVEAGIRGKNGFMREEFVDDEIYLVKVPEAIKDIAVLTEPLSNVVKAIEEVMHIQQRMVWNCEDGAYDCRNAYVVGTGPIGTFFSLLLRTYGFNVYMLNRRDPSPAEEYVSTRIEAEFVNTTKGVDHLPKADIIVDTSGFPSAFVPLMHKMNKNSILVLFGTQTGDKVQIDADLVTFMVENNIAVVGSVNANKWHFKSAVNYLTMWKEKYGDLLNRMITTVVPPENAKEILENKPKGEIKSVIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N0</accession>
    <name>F4B8N0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Citrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2111</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 8">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776897.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N0"/>
    <dbReference type="STRING" id="933801.Ahos_2111"/>
    <dbReference type="GeneID" id="10601611"/>
    <dbReference type="KEGG" id="aho:Ahos_2111"/>
    <dbReference type="eggNOG" id="arCOG04237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_2_1_2"/>
    <dbReference type="OrthoDB" id="21302at2157"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06118">
        <property type="entry name" value="citrate_synt_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011278">
        <property type="entry name" value="2-MeCitrate/Citrate_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024176">
        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01800">
        <property type="entry name" value="cit_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF4">
        <property type="entry name" value="CITRATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="371" mass="42531" checksum="DA59CC8526AEF0FC" modified="2011-06-28" version="1">MELRKGLEDIAIKETEITYIDGVLGRLYYRGYSIYDLAEFSNFEETAYLIWFGKLPNKKELQEIKEALAEEREIPEYITEFIKNVRKDANPMDVLRTAVSMLGIEDRSNEELWKKAVKLTAKIPTIISYFDRVRKGLDIVHPDPSLSHAENFLYMMRGEKPNPIDSRTMDVAMILHMDHEMNASTFACLVVASTLSDLYSAITAGISALKGPLHGGANAEALKQFMEIGSKDKVEEYILKKLESGQRIMGFGHRIYKAYDPRAKILKEYARNLAKEKGKMELFEIAEKVDEIGCKILGKKNIYPNVDFYAGLVFYFLGFDPDLFPTVFASSRIVGWTAHVMEYLKDNKLIRPKAIYKGEIGRKYINIENRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N1</accession>
    <name>F4B8N1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776898.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N1"/>
    <dbReference type="STRING" id="933801.Ahos_2112"/>
    <dbReference type="GeneID" id="10601612"/>
    <dbReference type="KEGG" id="aho:Ahos_2112"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259117_0_0_2"/>
    <dbReference type="OrthoDB" id="33385at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536:SF3">
        <property type="entry name" value="UPF0111 PROTEIN HI_1603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25084" checksum="FE778A2E22C2D2B4" modified="2011-06-28" version="1">MLKFSINKEELLFSKLLEIAENIKDSTSLLNSLYLDIFNTNYPDATAKMVKIKGIYERIAMIREDIISMLYGEAFLPDFKESMLTLTQSLYEIMKSIKDAGRAITSRKPDEKLCTTLQSNFVSYLSLIQDGAEKLVLMISLLKKDIKEAIRIGKEIQLIERNGDEIKDLMIQRLYENEKESDIISILQLKDVITFLDDILDGMEDSTLSVETLYATLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W3</accession>
    <name>F4B8W3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775749.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W3"/>
    <dbReference type="STRING" id="933801.Ahos_0947"/>
    <dbReference type="GeneID" id="10600419"/>
    <dbReference type="KEGG" id="aho:Ahos_0947"/>
    <dbReference type="eggNOG" id="arCOG08475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197233_0_0_2"/>
    <dbReference type="OrthoDB" id="41704at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="9195" checksum="8B23153F154EBC56" modified="2011-06-28" version="1">MTEEKQEQERRQTKRWDRFTWTVVVGPLAFFFVLSIGLALYLNNFGPWRAVVPVVIGFAIFFFIMGVFLRSKFGRLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X7</accession>
    <name>F4B8X7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0961</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775763.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8X7"/>
    <dbReference type="STRING" id="933801.Ahos_0961"/>
    <dbReference type="GeneID" id="10600433"/>
    <dbReference type="KEGG" id="aho:Ahos_0961"/>
    <dbReference type="eggNOG" id="arCOG00283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_430008_0_0_2"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF2">
        <property type="entry name" value="HELICASE HERA CENTRAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="5">
        <location>
            <begin position="157"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="582" mass="66674" checksum="D9C8CA40D92A5660" modified="2011-06-28" version="1">MSEGLFEEQIGRLREMKVITRQTADGRGSVSFRNFIVEFPINSKITAGKILAVKSLQNDYLLLEVADLLPVHYGMINLDGSIPKEIRDEVMRKVEEDWGRNDEEAWLDVYAYPIGYLLKIDGGVEFYKGYSPPIPGSTVRILSRELYEKFVCDKNGVEIGNIIGEDVKLKINMEKAINYHIGIFAFTGSGKSNLTASLVRRILKENKDTKVVIFDISLEYSILLLDQLISNNSRLISTERLPINKTDAGRRFIRTHVIPEEIIDLKDEIRKSAEELFESNKMRQLYVPPEGSTYLTYGEIIDMVRAQINDKYTAFAQKPLFGLFLKKIDETMRQNKLTKEDIADTTLSPVIDEIETLARESGLKENASIFAFLNALRAYLTSEIQEGEEYDIEKMAIEILDNEENSPRLFILELPNIEEARLIVGQLINQIMLRRKKSYSINPILFVLDEAQEFIPFDTKQKDNSEFSSNAVEKLLRHGRKYHLHGLISTQRLAYLNTNVLQQLHTYFISTLPRPYDRQLIAETFGINDTLMDRTLDLEVGQWLLVSFKAALPHDVPVFFTAPNNLEELRKGIQNSRPSSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Y6</accession>
    <name>F4B8Y6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved pro-fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776924.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Y6"/>
    <dbReference type="GeneID" id="10601640"/>
    <dbReference type="KEGG" id="aho:Ahos_2138"/>
    <dbReference type="eggNOG" id="arCOG06947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177745_0_0_2"/>
    <dbReference type="OrthoDB" id="37906at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054177">
        <property type="entry name" value="B129_C2H2-Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22034">
        <property type="entry name" value="B129_C2H2-zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="109" mass="12890" checksum="406452832E64147E" modified="2011-06-28" version="1">MKAKLAIKTKETQVNPLEFLKRAIENQFTPVSSDLQIYVSLRAVYKWVCPICLTPFETYQAFIMHLRYEEKEKTCRYCGKMFSKTDDLIDHIGKKHAWLPWGRSKSSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z2</accession>
    <name>F4B8Z2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776930.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z2"/>
    <dbReference type="STRING" id="933801.Ahos_2144"/>
    <dbReference type="GeneID" id="10601647"/>
    <dbReference type="KEGG" id="aho:Ahos_2144"/>
    <dbReference type="eggNOG" id="arCOG05960">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076116_0_0_2"/>
    <dbReference type="OrthoDB" id="37007at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="252" mass="28838" checksum="3EE8F50386170413" modified="2011-06-28" version="1">MQLVKNRISINSVNIPKDFSVIAYGKGMELPAYTLERSIINLEGKIIKTVPIHEFALVEDAYAEPGKVIFFINDQNESREILDNAWALNLEGYLFSCNEIKPKGKIEVIKFKGEMCEVNLSLSILYSEVSSIKGVRANRILKELDFSDIDDFLAEKSKEIDLSLPQIILSPILLPSRYFVEKNLNKDVKDYYSMNFSNDANIIYTGVDSIPMRRISFELRKKGKKVKEILLDVEPLLAPIYLSLMTYYMMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B905</accession>
    <name>F4B905_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase, subfamily IIA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776943.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B905"/>
    <dbReference type="STRING" id="933801.Ahos_2157"/>
    <dbReference type="GeneID" id="10601660"/>
    <dbReference type="KEGG" id="aho:Ahos_2157"/>
    <dbReference type="eggNOG" id="arCOG04221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_1_2_2"/>
    <dbReference type="OrthoDB" id="25155at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="261" mass="28976" checksum="B3EEFE1A4C5C6496" modified="2011-06-28" version="1">MLDYDLIISDVDGVIVREGEPIWENIFAIRKLKEEGKKIILVTNNSGFSRVLLSRQLNYLGLEITPNDIITSGLSAAIYMKRNTKVKSVYVIGEEGLVEEMKNFNFRVLSTEEVEENNPDAVVLGLDRLVTYDKLSTGMRCVARGSMFIVTNMDRLWPSKDGLKLGAGALASSIIYALKREPDFIAGKPNKWIIQVAMELTGIKDLSKVLVIGDQLETDIKMGNDIGADTVLVLTGISRKEDIEKSSIKPKIVVENLSLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B919</accession>
    <name>F4B919_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1152 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B919"/>
    <dbReference type="STRING" id="933801.Ahos_2171"/>
    <dbReference type="KEGG" id="aho:Ahos_2171"/>
    <dbReference type="eggNOG" id="arCOG04164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069296_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010581">
        <property type="entry name" value="DUF1152"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06626">
        <property type="entry name" value="DUF1152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029122">
        <property type="entry name" value="DUF1152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="41282" checksum="78B096651DB2015F" modified="2011-06-28" version="1">MTFKFKVRTQIIHAKSRAYYFSLAYSCKKYKVPKSILSNMKAFVFGIGGGGDILSALLPYVYYKRLGYEVKLGAVVWERYVEDPLPGPICKEDLVNFEEINDYVYKVRSNSYAIRGDLKVVPQLMRVLKVVNDYGFAICNKGGDIGMSEALNDLIDKEKFDLVIGVDAGGDVLANGGEKGLGSPLIDSISLASLVSLKVKSILGIIGAGSDGELDYEYILRRISEVAKIGGLLDSKGIDNDVANLMGEALKIVNTEASRIPFEAFHGLYGEVKIRNGTRKVYVNPVSSVMFFLDPKKLAQISGIYKIVAGSRSLDEANEKFHRYGIYTEYDFEKDLFEKFGFNSRNATYDELQRIREEGISRVKSGNVFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B954</accession>
    <name>F4B954_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776992.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B954"/>
    <dbReference type="STRING" id="933801.Ahos_2206"/>
    <dbReference type="GeneID" id="10601711"/>
    <dbReference type="KEGG" id="aho:Ahos_2206"/>
    <dbReference type="eggNOG" id="arCOG05950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639999_0_0_2"/>
    <dbReference type="OrthoDB" id="33859at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="165" mass="19254" checksum="66BA3EDDE0E59EEC" modified="2011-06-28" version="1">MDVVKEYLNLKPSKKIKILDIEIPCDTECLRNSNFKELLNNENFKEQLEILDGLENLIDDNINTLLQELEFKFSNYRVNLENLAYTIYKIVEEGGNVIVGNNSIIFEDKVIAKGDEFSSLYEVAKLIDEIKNDENIRSLCDEIKYLADSLWEHFNKNLRRALNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B966</accession>
    <name>F4B966_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B966"/>
    <dbReference type="KEGG" id="aho:Ahos_0973"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="99" mass="10851" checksum="2D7CDF3CB328ED26" modified="2011-06-28" version="1">MVAYGLSEADVDAFMKIVNSKEGKDVDAISSELGISKSRASLILKKLADAGLIEKQKSNGSKGGRPKYMYFVNKNEVIEKLVKRAEDVCKDLSEIIRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F0</accession>
    <name>F4B9F0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2225</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777011.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F0"/>
    <dbReference type="STRING" id="933801.Ahos_2225"/>
    <dbReference type="GeneID" id="10601730"/>
    <dbReference type="KEGG" id="aho:Ahos_2225"/>
    <dbReference type="eggNOG" id="arCOG01926">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_3_0_2"/>
    <dbReference type="OrthoDB" id="19205at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051312">
        <property type="entry name" value="Diverse_Substr_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053586">
        <property type="entry name" value="Glyceraldehyde_DH_medium"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041019">
        <property type="entry name" value="glyceraldDH_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42659">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42659:SF2">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="278" mass="30802" checksum="B94408964F87AF43" modified="2011-06-28" version="1">MYPPKIGYVIPDNLNEAIDFLKAHDDAKALAGGHSLIPMLKLRIIRPSYLVDITKLKELHYLNKEEKEYKLGATITHYEISKAQIPLLSEVASKIGDPQVRNMGTIGGSISQLDPSADYPAALLVLDAKVKIKGPSGERLEEFSSFDKDMFTPDLSQGEIVTEISIPVFPGYKYSYQKLERKAGDYAIVGVAVLIKTNGDEVEDFRVGVTGVNNKAYRSQIAEDMIKGKKINDEIIDKVAQEVANESNPTSDIRGSAEYKRKATKVMTKRAILDALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J7</accession>
    <name>F4B9J7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleotidyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777058.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J7"/>
    <dbReference type="STRING" id="933801.Ahos_2272"/>
    <dbReference type="GeneID" id="10601778"/>
    <dbReference type="KEGG" id="aho:Ahos_2272"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_2_2"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="3">
        <location>
            <begin position="306"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="414" mass="47568" checksum="B56D1D0E46F3EA04" modified="2011-06-28" version="1">MEWKIEDVKVIIPIGGEATRLRPLTIETSKATVRLLNRPLIEFSIYELAKQGIKEFIFGVRGYINYRSLFDTFKEGIGFSARYKIKPRVHFKYQPRVDSVGNADSVRINMDYYRINDITLIIQGDNIFRLDVKKLINYHLEKKAMMTIVLKKWDNVEEFGVADVDNDLRIKRFVEKPKKEEAPSNLINTGIYVLSPDIKKVFESEDVIKMREEGKMDFGKDIIPYLINHDYPVYGYITEDIWFDVGTPDRYLDAMQTLLATLPDSEIGGKRIDEDKRIFVQGTSPDSIRRRNIIISKYKKGKIKVEGNALIGRHCQIGNGIYIENSVIDNFTIVKNNVKIVKSSIMDRVYLGNNVEIQNSIIGRHVEIKDGVKIINSVIADDVTIGENSEIVNSRIYPHRVINSGSKLHDTMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K7</accession>
    <name>F4B9K7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777068.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K7"/>
    <dbReference type="STRING" id="933801.Ahos_2282"/>
    <dbReference type="GeneID" id="10601789"/>
    <dbReference type="KEGG" id="aho:Ahos_2282"/>
    <dbReference type="eggNOG" id="arCOG05947">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340783_0_0_2"/>
    <dbReference type="OrthoDB" id="33740at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="202" mass="23069" checksum="1D030770A787DA2D" modified="2011-06-28" version="1">MKVKLTWIYVPSDLLPHDEKDDDNDMEVIADGILEEFEKGEKEDLEIDERILIPASILSSRIIEDLPSNLSYFLGRWGGKYYSGDISGALGEIIVYTILEEKFGVKLLDILPLREAKFMGMITDTFIHIGKYEKLKEFLGDKDGKSILFVNVRSSVKFDKATVRKNITRDLITSESLRYPDNYSLLSYVFGDGNIMMVVVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L1</accession>
    <name>F4B9L1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc-containing ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_1041</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 11">Binds 1 zinc ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="10 11">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10 11">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775843.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L1"/>
    <dbReference type="STRING" id="933801.Ahos_1041"/>
    <dbReference type="GeneID" id="10600516"/>
    <dbReference type="KEGG" id="aho:Ahos_1041"/>
    <dbReference type="eggNOG" id="arCOG04548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178207_0_0_2"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009157">
        <property type="entry name" value="Fd_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000068">
        <property type="entry name" value="Zn_Fdx_Sulfol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10 11" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="2 10 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000068-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="11135" checksum="67A3828459577477" modified="2011-06-28" version="1">MGIDPNYRTSRQVVGEHQGHKVYGPVDPPKVLGIHGTIVGVDFDLCIADGSCITACPVNVFQWYDTPGHPASEKKADPINEQACIFCMACVNVCPVAAIDVKPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L6</accession>
    <name>F4B9L6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF929 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775848.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L6"/>
    <dbReference type="GeneID" id="10600522"/>
    <dbReference type="KEGG" id="aho:Ahos_1048"/>
    <dbReference type="eggNOG" id="arCOG03843">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090239_0_0_2"/>
    <dbReference type="OrthoDB" id="57485at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009272">
        <property type="entry name" value="DUF929"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06053">
        <property type="entry name" value="DUF929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26260" checksum="469B9774A39BC631" modified="2011-06-28" version="1">MKWKNLIILAAVAAFVLLFTLPYILYPFEVPLDTFFKVSNKDLAKPGYVCIILISWYGCPFGAADSWVLYSFLSHYGKIVYNFSYSDPQDVYPNTPAIIFKEFYPNSSVLFRFVYLYNRYLNATACCKVVSNYVSFGLSKISSCFPQYCPLVKEYVVNKWAQGGYFQSAAYMGNPPHIPTTILISSSKGTYILIGYIYNPSCISGMAPSYILTHLNSLSFIQSGVEKMENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N7</accession>
    <name>F4B9N7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="5">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">Ahos_1069</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>alpha-ribazole + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>alpha-ribazole 5'-phosphate + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775869.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N7"/>
    <dbReference type="STRING" id="933801.Ahos_1069"/>
    <dbReference type="GeneID" id="10600543"/>
    <dbReference type="KEGG" id="aho:Ahos_1069"/>
    <dbReference type="eggNOG" id="arCOG04338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="OrthoDB" id="11748at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148:SF1">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="177"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AEE93953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="244" mass="27121" checksum="F4D068ED5FAC4A3B" modified="2011-06-28" version="1">MKALKGVLSQLSFFTIIPSVDASLEEIAEYSFISPIFIGVITGVIDFVSYFSLFHILGYSSRFVIIIIVEVIRGFNHLDGLLDLGDAIMIKGDYERRLKALKDVAVGSGGIGLASVYFSLFLISLCGFPYPSIGTLLILISAEVLSRDLGILILSILEPMESSYLGKLFHDKLKDKWVIIIAEGIPFLLFYPFSVIIFFTLFLIFYEIGKKVLRGSSGDLTGAIITISFPLFLIVERLFSFLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P6</accession>
    <name>F4B9P6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P6"/>
    <dbReference type="STRING" id="933801.Ahos_1078"/>
    <dbReference type="KEGG" id="aho:Ahos_1078"/>
    <dbReference type="eggNOG" id="arCOG04762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040971_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053578">
        <property type="entry name" value="UDP-sulfoquinovose_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041015">
        <property type="entry name" value="UDPsulfquin_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="11"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="392" mass="45126" checksum="0BDB76BBEEC2FC63" modified="2011-06-28" version="1">MLTYEVLMRVLILGIDGYIGWALALRLVSKNHEVYGMDNLSTRKAVEEIGSDSAFPLPAPEDRVNAVKKYLDADIKFYKGDITDKEKLREVIKESKPDVIVDFAEQRSAPYSMKDLDHAVYTINNNLDGTLNLIYLVKEIDPSIHILKMGTMGEYGTPNFDIPESAFIEAIINGKKDKIFTPRWGGSVYHFSKIFDSYLLAYYNQLFGLTITDIMQGPVYGSRIPEIVNESLRTRFDFDEVWGTVVNRFCVEAVLGLPLTPYGKGGQTRGFISLEDSVEALRTLIEHPPKQGEYRVVNQFMEIHSVNEIAQMVKEAGEELGLHVEIKHVENPRVEAEEHYYNPERRILESLGFTPKRFMREEVKNMIEDLLPYKERLERFKDVIMPKTKWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P9</accession>
    <name>F4B9P9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P9"/>
    <dbReference type="STRING" id="933801.Ahos_1081"/>
    <dbReference type="KEGG" id="aho:Ahos_1081"/>
    <dbReference type="eggNOG" id="arCOG05925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165878_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="10965" checksum="3B532CF957FAAEE4" modified="2011-06-28" version="1">MTTDLPKDFNAPVDIEVDRDKGSVLLRNIIKDDPQNPLYIEYYIDKQFVENISKTRKIDIFFVNERFDELGKFEVKLMKEDLAIIRREIGLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R0</accession>
    <name>F4B9R0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_1092</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775892.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R0"/>
    <dbReference type="STRING" id="933801.Ahos_1092"/>
    <dbReference type="GeneID" id="10600566"/>
    <dbReference type="KEGG" id="aho:Ahos_1092"/>
    <dbReference type="eggNOG" id="arCOG01351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="OrthoDB" id="4488at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000005">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004976">
        <property type="entry name" value="PRK06349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331:SF1">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="13">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="140"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="313" mass="34474" checksum="C1DE7E3C25592589" modified="2011-06-28" version="1">MKLMLVGYGNVGKAFRKLLHEKKVKELENVKIAGIVTTRGLMLKDQEEFVPDKKINALEALDEVQPDVVVDMASANYNTGEPSLSLYLSALKRGIHVITTNKAPLALKYKEIINTAISHGSKIFFQATVMSGTPSINLYRILPAMKVRKIRGILNGTTNYILTRMSQGLSFNEALKEAQELGYAERDPTLDINGFDAAGKITILSNVYFSRYISINDVKFEGIGNIEIGDALKRGNKIKLIAYADEKETYVKPMEITKEDFLYNVDGVNNALEIESDIQKITIVGPGAGPLNAAYGAFSDLILLLRGENLPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X8</accession>
    <name>F4B9X8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleotide binding protein PINc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2323</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777109.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X8"/>
    <dbReference type="STRING" id="933801.Ahos_2323"/>
    <dbReference type="GeneID" id="10601831"/>
    <dbReference type="KEGG" id="aho:Ahos_2323"/>
    <dbReference type="eggNOG" id="arCOG00721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109674_2_0_2"/>
    <dbReference type="OrthoDB" id="27944at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="4">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="160" mass="18017" checksum="88AF18BA6BE86A29" modified="2011-06-28" version="1">MEVNRNIIFDTAGFLAGLENLLNKVYTTPYVINEVKDLASRNSLSLATSSGKVIVMEPSKNSINKVLKAIKEINEQTLSKTDISIIALSLDLSPSLVFTDDLSLQNVLLRFNIPFKSVKLNKMAINEKKFIYMCQECKRKFNLYLKECPYCGGKIVKTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA07</accession>
    <name>F4BA07_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777138.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA07"/>
    <dbReference type="STRING" id="933801.Ahos_2352"/>
    <dbReference type="GeneID" id="10601861"/>
    <dbReference type="KEGG" id="aho:Ahos_2352"/>
    <dbReference type="eggNOG" id="arCOG14119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264515_0_0_2"/>
    <dbReference type="OrthoDB" id="41259at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="25331" checksum="1C7940D630235FA2" modified="2011-06-28" version="1">MEEEFTLLLPSFNAYDFFSNPSNIIKFIPLFKSISQISDNEFIVSVGWILNIKINVKRILAKNRISYIISRSEQPRIMGKLDHIFEPLQGNTNLTKVKIIFYYKGPLEKLIKIESRRFYNKVKDEIDQNNKEEVSDIEFDNILNQMKTILSGIIKTDDQFDMLINKAVMESINSEIALIIGSDRNSMKFLFDKGNLIKEKGSVENIKSGMKFVMKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA08</accession>
    <name>F4BA08_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isocitrate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="4">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ahos_2353</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA08"/>
    <dbReference type="STRING" id="933801.Ahos_2353"/>
    <dbReference type="KEGG" id="aho:Ahos_2353"/>
    <dbReference type="eggNOG" id="arCOG01164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_7_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004439">
        <property type="entry name" value="Isocitrate_DH_NADP_dimer_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005036">
        <property type="entry name" value="PRK06451.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504:SF1">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="16">
        <location>
            <begin position="29"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>D-threo-isocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="336"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="14">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="14">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-succinyllysine" evidence="15">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="15">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="15">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604439-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE95224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="416" mass="47276" checksum="91B91F917D786BEC" modified="2011-06-28" version="1">MLREMYKIPEDGEKIQFKDGKWITPNKPIILYIEGDGIGPEIVKSTIDVINKAVELKYKDNREIKWVEVLAGNKAAQLVGDRFPKDTQDMLKEYRVVLKGPMETPIGKGWKSVNVAIRIMLDLYANIRPVKYIEGLESPIKNPERVNMIIFRENTDDLYRGIEYPYNSPEAEKIRKFIREELKVDVEDDTGIGFKIISKFKTQRITRMAIKYALQNSRKRITIMHKGNVMKYTEGAFREWAYETAINEFRDFIVTEEEVTTKYGGKIPDGKILINDRIADNMFQQIITRPEDYDIILAPNVNGDYISDAAGALIGNIGMLGGANVGDNAGMFEAVHGTAPKYAGKNVANPTGMIRGGELMLRFMGWIEAADLIDKAIVEAIKQKKVTQDLARYMNVKPLSTTEYTQELLNIMETLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA46</accession>
    <name>F4BA46_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="12">DGGGP synthase</shortName>
            <shortName evidence="12">DGGGPS</shortName>
            <ecNumber evidence="12">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_1160</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sn-3-O-(geranylgeranyl)glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775959.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA46"/>
    <dbReference type="STRING" id="933801.Ahos_1160"/>
    <dbReference type="GeneID" id="10600634"/>
    <dbReference type="KEGG" id="aho:Ahos_1160"/>
    <dbReference type="eggNOG" id="arCOG00476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="OrthoDB" id="11851at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13961">
        <property type="entry name" value="PT_UbiA_DGGGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="280" mass="30481" checksum="B3A5D58505FB8E0B" modified="2011-06-28" version="1">MNPYLKLVRIHNVIGAAIGDFTGYVVSSAWNFNLEKLIISLIVVSLVAAGGYAINDVYDVEIDKINKPDRPLPSGRISIKNAVTLSYSTMIIGSGLAFILGILQGLLAILTSIALIYYAKTLKRQGLPGNIIVATTTALSIFYGGIAYFEGNWFERVIIPTAYSFLLTLGRELVKGIEDYEGDKKYGVRTLATTKGIRFAWNTARVILLLMLAISPLPILLGFNIIYGILLIPFTFFTLKAIFAETSPRGGGEARGLLKGSAFIGMIAFILGSLPFKIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA84</accession>
    <name>F4BA84_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flavin-dependent thymidylate synthase</fullName>
            <shortName evidence="7">FDTS</shortName>
            <ecNumber evidence="7">2.1.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">FAD-dependent thymidylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Thymidylate synthase ThyX</fullName>
            <shortName evidence="7">TS</shortName>
            <shortName evidence="7">TSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">thyX</name>
        <name evidence="8" type="ordered locus">Ahos_1198</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH(2) as the reductant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H(+) = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:29043"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.148"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the thymidylate synthase ThyX family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.148" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775997.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA84"/>
    <dbReference type="STRING" id="933801.Ahos_1198"/>
    <dbReference type="GeneID" id="10600674"/>
    <dbReference type="GeneID" id="42778249"/>
    <dbReference type="KEGG" id="aho:Ahos_1198"/>
    <dbReference type="eggNOG" id="arCOG01883">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077585_0_0_2"/>
    <dbReference type="OrthoDB" id="18918at2157"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050797">
        <property type="term" value="F:thymidylate synthase (FAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20175">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.170:FF:000004">
        <property type="entry name" value="Flavin-dependent thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01408">
        <property type="entry name" value="ThyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003669">
        <property type="entry name" value="Thymidylate_synthase_ThyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036098">
        <property type="entry name" value="Thymidylate_synthase_ThyX_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02170">
        <property type="entry name" value="thyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34934:SF1">
        <property type="entry name" value="FLAVIN-DEPENDENT THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02511">
        <property type="entry name" value="Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69796">
        <property type="entry name" value="Thymidylate synthase-complementing protein Thy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51331">
        <property type="entry name" value="THYX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Involved in ionization of N3 of dUMP, leading to its activation" evidence="7">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="87"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <note>ligand shared between neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="29812" checksum="E3BBF20474BDABEC" modified="2011-06-28" version="1">MKVELVSYTKDGERVVAIASKMSRSRKGWDYHEKTMTDEEVEVWIRDAIIHGYWSPLEHSSYTFSIEGISRVASHQLVRHRIASYTQMSHRFAKPVDEYYQPVTPPSAEKRNEELVKKAYDDAYKYYYELLQNGVPEEDARYVLPNGVNTNIVVTMNARELYNFFALRLCSRAQWEIRAVAWKMLEEVKKVHPRLFKYAGPNCIIHENFIRETPISLDELNDKTEFLSQRCVEGVPRDGILKCIQNAKSILNKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA85</accession>
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        <recommendedName>
            <fullName evidence="4">DUF2175 domain-containing protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="10600675"/>
    <dbReference type="KEGG" id="aho:Ahos_1199"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="57156at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF09943">
        <property type="entry name" value="DUF2175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="43"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13041" checksum="96FE5BAB808CE8D5" modified="2011-06-28" version="1">MSRAPPTKWTCDICKNTIYWDELFTFTSKKTVVHYTCFREKALKTAKVDQEQLTAILDSLEDELKMIVVYKQRLGKIKDEEVKKYMEQAEKDAEKNSAMLTRAVEKLSGVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3K8</accession>
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        <recommendedName>
            <fullName evidence="1">DUF6788 domain-containing protein</fullName>
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    </protein>
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    <organism evidence="2 3">
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_0002"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF20586">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6788" evidence="1">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="13116" checksum="84DE528DA95DAF40" modified="2011-06-28" version="1">MLWEKVKELEEKFLQIQKEREKLEEELKRLPTGHIDKKKIGNHVYYYLRYWEEGKLKSKYLGKDAEELQQKIEYSMDIRKKVSLLREEEKRLQKILDRIKNSLEFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M7</accession>
    <name>F4B3M7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Spermatogenesis-associated protein 20-like TRX domain-containing protein</fullName>
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    <gene>
        <name evidence="2" type="ordered locus">Ahos_0021</name>
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    <organism evidence="2 3">
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        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M7"/>
    <dbReference type="STRING" id="933801.Ahos_0021"/>
    <dbReference type="GeneID" id="10599461"/>
    <dbReference type="KEGG" id="aho:Ahos_0021"/>
    <dbReference type="eggNOG" id="arCOG02007">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
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        <property type="entry name" value="Ssp411"/>
    </dbReference>
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        <property type="entry name" value="Ssp411-like_TRX"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899:SF1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="2"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="642" mass="73180" checksum="E24BEAEB073737F7" modified="2011-06-28" version="1">MNSLANSESLYLREASNQPVNWLPWSKEAFEKAKKENKLILVDVGAAWCHWCHVMDEETYSNQEIAKIINENFIAIKVDRDEMPEVDRKLQLIVSEISGESGWPLTVFMTPEGNVFFGGTFFPPEDSYGRIGFKRLLNEILRIWNTERDKILNSSIPLSIKVKTENEGQLNKDLIESSFSLLTSAYDLEYGGLGKSMKFPHPKVDEFMLAYSAWTGDDLGKKLNNFTLIKMFEGGIFDQVGGGFHRYTTDREWLTPHFEKLLIDNAELLEDYYIAYLATRKVEFLEAYNLTYEFIKRDLENSIAFSNSIDADSNGIEGGYYTWTEEEIKDALGQEAELAIKLFGLRKEGGVVEDRKVLRVDLTEAKKLINADVNITLQKLRDLRRKMLEYRQKNRKMPRIDTNSYSYPNYRLAEVLFQSLKIDDGLKIIKNLTPIISRRLTGGKEGLLEDYASALLASISAYEVTAEERYYSLSLELYNKLKDFFKADGFEEPNGELPYGDMPNESANSLAVRGILKLSMLYKIDIEIEKILSKLVNSDPSFVSGLLLSAGSIINGIAHVVIVDEKDGRAEVLHKEAITTYYPLKVVEKISDDIKDRLDSTLRAMLNENKGKSRAYVCIGNTCSMPVFEKEKIKLLLKTKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N7</accession>
    <name>F4B3N7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0031</name>
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    <organism evidence="1 2">
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N7"/>
    <dbReference type="STRING" id="933801.Ahos_0031"/>
    <dbReference type="KEGG" id="aho:Ahos_0031"/>
    <dbReference type="eggNOG" id="arCOG05127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104927_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044036">
        <property type="entry name" value="DUF5752"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19027">
        <property type="entry name" value="DUF5752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="25291" checksum="53E85A64D8868D91" modified="2011-06-28" version="1">MNLDSQGKGIPFKFYAAYYPPVYTKVKANSLSELIKGISTVDKNSIFYHVFHPMLSSHVVPEDLPNDFAFWLRESLHDEYLAEVVADIKGREPLTIEDIRKELLELLNTSTHLDKTADYPFVFISFIPVVYPLDVEVRTLAEFMDAIAKVPVRSLFYHFVYKRVMGLSKRNDFTTWLEENFGLIDLGEKLAKIDPQTYTEEEDFRQDLLKILEGELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R7</accession>
    <name>F4B3R7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1221</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R7"/>
    <dbReference type="STRING" id="933801.Ahos_1221"/>
    <dbReference type="KEGG" id="aho:Ahos_1221"/>
    <dbReference type="eggNOG" id="arCOG05903">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_898978_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="29232" checksum="6FE8D6227ADF490F" modified="2011-06-28" version="1">MSIISLRDKYMVDISYKSAFKARLLGNASSLLIPGWAGQELARAAIYNLEKKNLIESLSLSIAEAPFDVISGAILFIIILPIKFYYIEFLYVLVALGNIVGWSIGIAYVYSTAGRHVETEKRLMKLIGMEKYYFLLLQGKDSIRNSIGNKRFIMYMALSFLGYLVLSAGLYPLIPNYFYDIVVTMAYFAATLFPIPGASGVSELALAIVLPSSYVFDIIILEYVSYALGFIFLREISFSELKKDIDKIKKDGELYKRADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S6</accession>
    <name>F4B3S6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S6"/>
    <dbReference type="KEGG" id="aho:Ahos_1230"/>
    <dbReference type="HOGENOM" id="CLU_3112838_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="50" mass="5939" checksum="5D1D07991C4BE657" modified="2011-06-28" version="1">MKTVKKGEDTFVEFSDFNEVREVFEEAIKEIEEEEKANARRPSYEIQSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T6</accession>
    <name>F4B3T6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776039.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T6"/>
    <dbReference type="STRING" id="933801.Ahos_1240"/>
    <dbReference type="GeneID" id="10600719"/>
    <dbReference type="KEGG" id="aho:Ahos_1240"/>
    <dbReference type="eggNOG" id="arCOG01736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_2"/>
    <dbReference type="OrthoDB" id="44091at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:RNA 2',3'-cyclic 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561:SF1">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="12"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="92"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="182" mass="20645" checksum="B9D8CD1DD8C5B00A" modified="2011-06-28" version="1">MRLFIAIKVPQFQKLNDVYNEIKLSGADVKLVETDNIHITLIFIGEVPDNKVDLVKEAVSLLQFNKFKIAMKGMGAFPSITKPRVVWVGISEGFPQLREIRSFLLKELRSRGIRPEDEKEFVPHITLGRVKGPSNLFNLANVINQHYNDYFGDFIVDKVILYKSTLTPKGPIYDEILGVAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T9</accession>
    <name>F4B3T9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease Z</fullName>
            <shortName evidence="8">RNase Z</shortName>
            <ecNumber evidence="8">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnz</name>
        <name evidence="9" type="ordered locus">Ahos_1243</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T9"/>
    <dbReference type="STRING" id="933801.Ahos_1243"/>
    <dbReference type="KEGG" id="aho:Ahos_1243"/>
    <dbReference type="eggNOG" id="arCOG00501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000801">
        <property type="entry name" value="PRK00055.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="288" mass="32643" checksum="9462A3036A3E9B7F" modified="2011-06-28" version="1">MEVYFIGTGGGAPSKRGLPAFLVRRQGFNVLLDCGEGTQITMINHGLNIMAINLIAITHLHADHVLGLPSLIQTMGMYSRKEKLYIIGDVKDLLNKVFEETYFEPPFKIEYVDSYEDNELKITPFKTCHVVKSQGYLIEEKDKKNIDIEKLTKEGIKDWRIIRQLKAGKEVIFNGRKLYPEDYLITKKGIRIAYTGDTAICDSVINSVKGVDLLIHDSTFLDDINAKEYGHSTVTDAATVAKEAGVKRLALVHISGRYKDAKIMEEIAKRTFWKSFLPDDLSYYILKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U8</accession>
    <name>F4B3U8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Translation initiation factor 2, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776051.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U8"/>
    <dbReference type="STRING" id="933801.Ahos_1252"/>
    <dbReference type="GeneID" id="10600731"/>
    <dbReference type="KEGG" id="aho:Ahos_1252"/>
    <dbReference type="eggNOG" id="arCOG04107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
    <dbReference type="OrthoDB" id="84794at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003062">
        <property type="entry name" value="PRK03987.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="262" mass="30020" checksum="4BFA261EA68AA754" modified="2011-06-28" version="1">MIYNKHPLPREGDILIATVKQVFDYGSYVTLDEYGNLQAFLPWSEISTRWVKNIRDVIKEGRKIVVKVIRVDRKKGTVDVSLKKVNDDERRKKNAEWKKLQKTDKILELVSQKLGKTEKEAWEQVAWKLESKYGDVFDALQKAVKEGDKILRDAGVPEIWIKPLIEEASKHSEEKKVKESKVVVVKTLDPEGVEKIRSLFGKAVEGEEDTDFTIKIYSIGAPRYRVDVTGTEPKAVAERLNSIIEKLQEIAKEEGVEISVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X0</accession>
    <name>F4B3X0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="4">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GTP cyclohydrolase I</fullName>
            <shortName evidence="4">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">folE</name>
        <name evidence="6" type="ordered locus">Ahos_1274</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homopolymer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776073.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X0"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:GTP cyclohydrolase I activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="GTP cyclohydrolase 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK09347.1-2"/>
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    </dbReference>
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        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="5">
        <location>
            <begin position="14"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="166"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="212" mass="23829" checksum="C6C216E7A13B1282" modified="2011-06-28" version="1">METSISQEKLVEEIAKRVREILELLGEDPNREGLRETPERVARALIEMTSGLRSPPPKIKVFNAKDIGEDITEDENGDQVILIRDIQFSSLCEHHLLPFIGRIHVAYVANGDGKVAGFSKIIRIVNYYSARPQIQERLVSQIADAIMNSEIKPKGVMVIGNGIHMCAYVRGVKDKEAKLVSVATRGVFKTNRALVNQVFRLLDTTHERSLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X1</accession>
    <name>F4B3X1_ACIHW</name>
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        <recommendedName>
            <fullName evidence="4">DUF1614 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774852.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X1"/>
    <dbReference type="STRING" id="933801.Ahos_0040"/>
    <dbReference type="GeneID" id="10599480"/>
    <dbReference type="KEGG" id="aho:Ahos_0040"/>
    <dbReference type="eggNOG" id="arCOG02159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082100_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011672">
        <property type="entry name" value="DUF1614"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07758">
        <property type="entry name" value="DUF1614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27298" checksum="62B60615E3F04A76" modified="2011-06-28" version="1">MGKRIVIFYPFRGILFPVYLFMGLILVLVSIGYFKDLLLFVGVSRKLSYLFAFEISFLSLLLSPVNFVIKEVKKRAIAPRYDVVYVFGIPFYVPRLDIEYFTTQVAINFGGALIPLMLSISLLFLLYKYLLPIFINIILLIIVSKYFSKVIEGVGVVMHPFIPPLFSVLFSYLLFFKMPELIPVSAYISSVLGTLIGADLLNLRKIIDASPQIVSIGGMGSFDGIFLSGLFSVLLGELIISIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X9</accession>
    <name>F4B3X9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774860.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X9"/>
    <dbReference type="STRING" id="933801.Ahos_0048"/>
    <dbReference type="GeneID" id="10599488"/>
    <dbReference type="KEGG" id="aho:Ahos_0048"/>
    <dbReference type="eggNOG" id="arCOG05904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029035_0_0_2"/>
    <dbReference type="OrthoDB" id="34497at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="265" mass="30812" checksum="598B0684F10884F5" modified="2011-06-28" version="1">MNPSSLYKLLDSPIKKDAEDAINYCKNNQLVPLLSFYLEDELLNNLVKSLDKDFYNLYIEYKYNKTFFIKKIKEKFNAEKDYEDFPYYLVPIGENNKVMIVNNDNVPPKAVPIEGKFRLTFLIHSSFDELNHDILSQSDDDIVLEFKNGELVNIEKKRNIFMDSRSVEKIEESRVFKSNLIVPGYLLLVSVVSNNLFPYHNILTINIGENGKVSVSIENGKATQEDVINGKTLTAEEKAKIYFEYKQKQIIKEEILKSIIWKLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B427</accession>
    <name>F4B427_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0098</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774908.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B427"/>
    <dbReference type="STRING" id="933801.Ahos_0098"/>
    <dbReference type="GeneID" id="10599538"/>
    <dbReference type="KEGG" id="aho:Ahos_0098"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF18">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE92991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="308" mass="34994" checksum="811ADD41F246822E" modified="2011-06-28" version="1">MIETVDLTKIFKIKRKEIKALDSVSINVEKGKIGALVGHNGAGKTTLLKILSTLVLPTSGDAFINGYSVTGEEKKVRENIGLVTVSERAFYYRLTALDNLIFFSSLQGLSLGDARKRANQLLDFVGLSEWKNVPYMKFSTGMQKKLALARALITDPPVLLMDEPTLGLDLVSAREFRATIRKLKKKNKTILLSSHYLREIEELADIIFLIKRGKIVASGTLDELKRMTGKVLEVITHEINNEIRKFVIYSTDKEYILRIPETEIDKVTVHYKEVEEKDPTLDDIYIHFVGEVEEDIGRGRSRPWRLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B438</accession>
    <name>F4B438_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774919.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B438"/>
    <dbReference type="GeneID" id="10599549"/>
    <dbReference type="KEGG" id="aho:Ahos_0109"/>
    <dbReference type="eggNOG" id="arCOG08312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565908_0_0_2"/>
    <dbReference type="OrthoDB" id="33933at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="83" mass="9277" checksum="26B2781A3F0F9FC8" modified="2011-06-28" version="1">MEEAINLAKMGKPLTAMLLIKSYVQEKIEEGKDVNKMDKICRDLISAILATPSINDESWRVFVPSPSLEEIEAVVQKVKECLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B449</accession>
    <name>F4B449_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-methylhydantoinase B/acetone carboxylase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774930.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B449"/>
    <dbReference type="STRING" id="933801.Ahos_0120"/>
    <dbReference type="GeneID" id="10599560"/>
    <dbReference type="KEGG" id="aho:Ahos_0120"/>
    <dbReference type="eggNOG" id="arCOG01512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_0_0_2"/>
    <dbReference type="OrthoDB" id="8261at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF23">
        <property type="entry name" value="HYPOTHETICAL 5-OXOPROLINASE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="507" mass="55375" checksum="B088A475F61FCBF9" modified="2011-06-28" version="1">MKWEIVHKATSFIAEEMGVSLKKSALSPNIRERMDHSCAIVNEEGKIVAQAEHIPVHLGSFKIGVLNVLNYLEREGIELEEGDSIIFNDPYISGTHLNDVGVLSPIFYNGKLVGYAVNKAHHVDVGGPLPGSINPTAKTIYEEGVVIPPVKLTRKGSLNDEIIKIIKENFKVPDFSLGDIKAQISANKLGEERVRQLFEKYGDIRQSWEESIDYSRRLALNVINTWKKGEYEAEDYLEWGSSLLPIKIKLQIEEKGIIADFEGTHKQIEGPLNAVLGVTYSAVSFAVRSMVGEVPTNDGFYSLITVKAEEGSLVNPLKPAAVGGGNVETSQRIADVTFLAMSKFLKVPAAASGTMMNIMMGSIYKGKYWSYYETVGGGSGGRPCKDGVAAVQNNMTNTLNTPIEIAERQYPILFIEYRIREGSGGNGLHKGGDGIIRGFKLLSKAKISILADRFKIGPWGLEGGERGKPGRVTINGKDYPSKFTEEVNEGDVIIVETPGGGGYGKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C9</accession>
    <name>F4B4C9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Helicase, superfamily II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0125</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774935.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4C9"/>
    <dbReference type="STRING" id="933801.Ahos_0125"/>
    <dbReference type="GeneID" id="10599565"/>
    <dbReference type="KEGG" id="aho:Ahos_0125"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453163_0_0_2"/>
    <dbReference type="OrthoDB" id="39583at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF6">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="9"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="210"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="602" mass="69396" checksum="F00908646D463C16" modified="2011-06-28" version="1">MSNFVKLDIKALDSYQPLMLISPTGSGKTFSVIKYALKQNTYNKVVFAMPTKAAIREVYTKLSLFTNDVGRDDSDARLEDNFNAEEVWRKKIIVTSYERLLQQMISSPSLFSNSLLVIDEAHLLLSEGRNCTLQELLAYYKFFREKFSGKINVILLSATMPDVDSLSEYLEAKVICNDKRPVNIDVKIVKVSQKRGENYYLNKAIEFINYVKKGELKLENYKQILVYTNTRRSAEEISKLIEKELHVPTAFHHAGLPIEVRKKIEDDMRSENNQYKVIVSTDTLALSINTNVDVVVVLALKRFAGLRTYVEPSTIAQVIGRAGRPGYSEKGLALVFAEPDEVKIVNKALKKEFGKVPEPLDYPQTVLRWIYSGLDPLLLARYGYRYSPSKIKDSLLFLENIGAIQKGKVTPLGKVFAYEMVPKKGMNLLKLIIKFDKKGNPLVRALYYSFIYSFFVDEYFSRKVNEGEILRKGNSIFLDLVKYLRGSFGKYTYFTVGETLEPSTWFYYSLTVPQVISTDDIAESIRKSSEIIYNLSTYSLISEDLNKPSYFIMRLMRSYRRILREGKKGEEIIDFIRFCFSNYQELSKSKDEFFDTQWIRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F6</accession>
    <name>F4B4F6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0152</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774962.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4F6"/>
    <dbReference type="STRING" id="933801.Ahos_0152"/>
    <dbReference type="GeneID" id="10599592"/>
    <dbReference type="KEGG" id="aho:Ahos_0152"/>
    <dbReference type="eggNOG" id="arCOG02687">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061946_0_0_2"/>
    <dbReference type="OrthoDB" id="43373at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="377" mass="40731" checksum="6C32AB64EFBF0FD5" modified="2011-06-28" version="1">MLTKKQLALISVGTSLSFWDIFNVPYIENEASAQLGSINSTLILSAEMIGYFTGGAINGLIASKYGRKIGIILSMLIIFLGSTIGFFSTSFLQLAIAEFIIGFGIEGEVAVVPAYVSEMSSANFRGRAVGFTTLGGFLMSLVVGPVAVIAGNDWRILFLPSLIISAFALIFRITLPESKMWEEKRREKMVWDNTVLLFLAIWFTSYFAGYSLFSTPVFSLLSNKGFSNTSLYFTYILYGDPIGVTLASILNDKLERKFSSSASNLLSGIAIISMPLIGGISLLIAGFIAMFFQGFKFPTMYAYTAENLGTKIRSLGYGIADGIGHLGGAVGPILFTLTYGYNAGYSFLLIGIVSMISGVMLLKGARVKGKSLEEIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P3</accession>
    <name>F4B4P3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative HTH-type transcriptional regulatory protein Ahos_1390</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776188.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P3"/>
    <dbReference type="STRING" id="933801.Ahos_1390"/>
    <dbReference type="GeneID" id="10600879"/>
    <dbReference type="KEGG" id="aho:Ahos_1390"/>
    <dbReference type="eggNOG" id="arCOG04152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075726_1_0_2"/>
    <dbReference type="OrthoDB" id="31424at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="MTH_967-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059051">
        <property type="entry name" value="MTH_967_PDDEXK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26553">
        <property type="entry name" value="PDDEXK_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="129"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="298" mass="33885" checksum="397D9802AA7F9FA0" modified="2011-06-28" version="1">MTIRVNDVIRILEEEGLSYTIINYPEKNKKSIDIIVEFKRSSQEKKKLVVKTSSDKIGKDEITDLKNFSSLTQSIPLIITDETEEDIAIEKDKVIGLSLFGFERLLKGDKIFVYRTRGGMFVRIRPEVLRQKMREMNYSMGDVAKMLGVSRKTIYDYENGDSDVSIEIAEKLIDIFGPEIIGDVCETYQADGKVSVDKDHKVINILESKGFKVATLKFTAVDIIASNNSKKLIITIESRNPENSIKKIQEASKIAEEFNLNMIVISKSSSRAKELEKDGFKVYLDQNLDELKYEISGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V0</accession>
    <name>F4B4V0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775032.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V0"/>
    <dbReference type="STRING" id="933801.Ahos_0223"/>
    <dbReference type="GeneID" id="10599663"/>
    <dbReference type="KEGG" id="aho:Ahos_0223"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
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        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRANSPOSASE-RELATED"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="298"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="382" mass="45018" checksum="3FB9D556E6206284" modified="2011-06-28" version="1">MARRGNKAIRATVSMKIALSDSLLALVNNYVKAIRYTLFWLKENVPNPNEKGVLSKVHEELYTRLKGEYNLPSKVAQDCYRDALSMYKGWYNNPRRGRFPRVYKPTVWLTPKASYNVDFEAMTVRIASVDELPILGYPRNLKEYMSWRMREARLTVKDGKAFLKVVFEKEEENVELKESIAIDINMAEVVVGKDDTHYVRIPTRLDEVHHWKSLAENLQRKYPRRWRENKRVLHRIYSFHQKARRIAEDFARKVGKWIVDVARVMGINVIKLESLKNLIKNVDKLPKEFHDKLYLMQYRRLQYWISWQARKHGIIIEIVNPSYSSVSCPKCGKRMEEKGYRWFKCNCGYENDRDVIAVVNLNGRGSLTLSTAPQMRDVIPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z0</accession>
    <name>F4B4Z0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0264</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="STRING" id="933801.Ahos_0264"/>
    <dbReference type="GeneID" id="54122935"/>
    <dbReference type="KEGG" id="aho:Ahos_0264"/>
    <dbReference type="eggNOG" id="arCOG00818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_2_2"/>
    <dbReference type="OrthoDB" id="30861at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
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        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
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        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF7">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, SPOVT_ABRB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="8928" checksum="6EAD11561F2D635B" modified="2011-06-28" version="1">MEVKVHKKGIIVIPAEVRRRLNIKEGSVIELEVEGDKIILKRKLTLLDAYGIDKEMGDSAVKELEKLRKEEVEKENSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B510</accession>
    <name>F4B510_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate kinase</fullName>
            <shortName evidence="11">UK</shortName>
            <ecNumber evidence="11">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Uridine monophosphate kinase</fullName>
            <shortName evidence="11">UMP kinase</shortName>
            <shortName evidence="11">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyrH</name>
        <name evidence="13" type="ordered locus">Ahos_1429</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>UMP + ATP = UDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776227.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B510"/>
    <dbReference type="STRING" id="933801.Ahos_1429"/>
    <dbReference type="GeneID" id="10600921"/>
    <dbReference type="GeneID" id="42778487"/>
    <dbReference type="KEGG" id="aho:Ahos_1429"/>
    <dbReference type="eggNOG" id="arCOG00858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="OrthoDB" id="372251at2157"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000030">
        <property type="entry name" value="Uridylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833">
        <property type="entry name" value="URIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42833:SF4">
        <property type="entry name" value="URIDYLATE KINASE PUMPKIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="6"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="113"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="226" mass="24882" checksum="F849A92A0E28FE55" modified="2011-06-28" version="1">MKLILKLSGKIFDEEDPQKLLTLKEVVKELVNEGNRVGIVTGGGSTARKYISLARKMGSNEAYLDLLGIWASRLNAYLVVFSLNDLAYMKVPESLEDFIQAWSYGKVVVTGGFQPGQSTAAVSALVAEASNADLLIIATNVDGVYDRDPRIYKDARLLTKINTEKLKEILEGTQSVKAGTYELLDPMAIKIVERSKIKVIVMNYNKLGKLKDIIAGKEISTLVEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B512</accession>
    <name>F4B512_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10 11">Homocitrate synthase</fullName>
            <shortName evidence="10">HCS</shortName>
            <ecNumber evidence="2 10 11">2.3.3.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_1431</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the aldol-type condensation of 2-oxoglutarate with acetyl-CoA to yield homocitrate. Carries out the first step of the alpha-aminoadipate (AAA) lysine biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the alpha-IPM synthase/homocitrate synthase family. Homocitrate synthase LYS20/LYS21 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="2 10 11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776229.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B512"/>
    <dbReference type="STRING" id="933801.Ahos_1431"/>
    <dbReference type="GeneID" id="10600923"/>
    <dbReference type="KEGG" id="aho:Ahos_1431"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_0_2"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00028"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02222">
        <property type="entry name" value="Homocitr_synth_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011872">
        <property type="entry name" value="Homocitrate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02146">
        <property type="entry name" value="LysS_fung_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002085">
        <property type="entry name" value="PRK00915.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF63">
        <property type="entry name" value="HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="12">
        <location>
            <begin position="6"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR02146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="462" mass="50859" checksum="0A3F936468CEE865" modified="2011-06-28" version="1">MRFLKVGILDSTLREGEQTPGVVFTTEQRVEIAKALSELGVSMIEAGHPAVSPDIYEGIKRIVKLKKEGEITPEIVGHSRAVKKDIEIAAELEVDRIAIFYGISDIHLKAKTGKTREEALNIIAEAVSYAKEHGAKVRFTAEDATRADLEYLTQVIKTARDAGADRIGIADTVGILYPAKTRELFSYLSKIPGVEYDIHAHNDLGLAVANSLAAVEGGATIIHATVNGLGERVGITPLQVIAAALKYHFNVEVVKLELLPKISSLIEKYSGIPTPPNFPITGDYAFLHKAGIHVQGILNDPRTYEFMPPETFGRNRDYVIDKYTGKHALKDRFERLGVSLSEEELEQVLAKIKSNPNVRFYRDVDLLEIAEEVTGRVLKPRPPEKVEALISIKCDSNVYTTAVTRKISVIPGVKEVMEISGDYDILAKIEAKDPIELNIIIESIRSVKGVSSTLTSLVLKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B533</accession>
    <name>F4B533_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1452</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776250.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B533"/>
    <dbReference type="STRING" id="933801.Ahos_1452"/>
    <dbReference type="GeneID" id="10600948"/>
    <dbReference type="KEGG" id="aho:Ahos_1452"/>
    <dbReference type="HOGENOM" id="CLU_1656772_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="159" mass="18599" checksum="8A9E49A7FF631F4E" modified="2011-06-28" version="1">MIYAFTYLGDNFYIFWLGTKVGTITSDKLTYKGIAEIYIGKKIDNNLTPRDVNELIRSWYEAFRVGKFSRYPLEWLLSVVNELESDEKIQRILLKRMEKDIESEDEKKALTILGDDAITEINNTIGNSPFTKIAFTDRLYAYITRPPYILKPYVFTDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B544</accession>
    <name>F4B544_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1463</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776261.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B544"/>
    <dbReference type="STRING" id="933801.Ahos_1463"/>
    <dbReference type="GeneID" id="10600959"/>
    <dbReference type="KEGG" id="aho:Ahos_1463"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146475_1_0_2"/>
    <dbReference type="OrthoDB" id="53273at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14359">
        <property type="entry name" value="UBA_AeNAC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
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        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00264">
        <property type="entry name" value="archaeal-type nascent polypeptide-associated complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="5">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="NCBIfam" id="TIGR00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="114" mass="12582" checksum="E332E95D9B6B627F" modified="2011-06-28" version="1">MKVPKDLKALQRMGIKAEKIDALRVTIETPDEIIIIESPMVMKTSFAGQEAIVVSGGETKVEKKGEQQKVEIKDEDVKFVMEQTGKPENEVREALVKANGDIAKAIMILNGQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B581</accession>
    <name>F4B581_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775091.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B581"/>
    <dbReference type="GeneID" id="10599724"/>
    <dbReference type="KEGG" id="aho:Ahos_0284"/>
    <dbReference type="eggNOG" id="arCOG12783">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582821_0_0_2"/>
    <dbReference type="OrthoDB" id="39455at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="123"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="168" mass="19318" checksum="B4A01E87AC4158FE" modified="2011-06-28" version="1">MANLTLFKALLLIGFEKVAPRTLKRGDVTITVTFIPNVRWIVRLPHITYELSTQKEVLHKLVHEGIISRKELEYLASIGLDIAKEEIVQSEEITTGSLIDVRRAFITQVIMPRLEILLRTNGMKCPVCGKRFRSTMEFYNHLNTTEVRSEEHKKILEGIYEEVTGIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B5</accession>
    <name>F4B5B5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SAF domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775125.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B5"/>
    <dbReference type="STRING" id="933801.Ahos_0318"/>
    <dbReference type="GeneID" id="10599759"/>
    <dbReference type="KEGG" id="aho:Ahos_0318"/>
    <dbReference type="eggNOG" id="arCOG05318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084161_2_0_2"/>
    <dbReference type="OrthoDB" id="214896at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019698">
        <property type="term" value="P:D-galacturonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11613">
        <property type="entry name" value="SAF_AH_GD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044144">
        <property type="entry name" value="SAF_UxaA/GarD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052172">
        <property type="entry name" value="UxaA_altronate/galactarate_dh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536:SF5">
        <property type="entry name" value="ALTRONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30536">
        <property type="entry name" value="ALTRONATE/GALACTARATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAF" evidence="2">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="99" mass="11190" checksum="BE4D8BFD5DEDE03A" modified="2011-06-28" version="1">MPKGLIHSKDDDVCVVTNDVKKGDEVICAYLENPSSYVIIKSEDDIPLGHKIALRDIKKGEKVLKYSRPIGVAISDIRKGEHVHTHNLKSLRWGKWQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C2</accession>
    <name>F4B5C2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mandelate racemase (MR)-like subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775132.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C2"/>
    <dbReference type="STRING" id="933801.Ahos_0325"/>
    <dbReference type="GeneID" id="10599766"/>
    <dbReference type="KEGG" id="aho:Ahos_0325"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_2_2"/>
    <dbReference type="OrthoDB" id="42605at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010624">
        <property type="entry name" value="PRK14017.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF2">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00908">
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        <location>
            <begin position="130"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="381" mass="43338" checksum="4715121F3ED6CA21" modified="2011-06-28" version="1">MKIKDVKVYLVNAVWRNFVIVKIETDEGITGYGEGTMGDFEKTVEAAVYDFKPFLVGREVNVQEIYNFLYRHFFWRGGEILMTAISAIEQALWDIIGKSVNKPVYELLGGKAKDYVRVYANGFISGDRSPEEFANAVRSMVERGFNAVKIDPFGSAGPQISGEELEKARNRLKAIRESVGYDVDVMLDAHGRFNVASAVKVANEVARYEPYWLEEPVPEEDIKALKYVKERSPITIASGERIVNKYRYREFLEERAADIIQPDVCHVGGIWALREVASMAETYYTSVAPHNPNGPIATAATLNLMLNMNNSLIMEFWVDAETVRRALLKEYFTIKGSYIYPSGKPGLGIEVNEEALEKFPYKKLHLEYFSKDYKYYGDVNQ</sequence>
</entry>
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            <taxon>Thermoprotei</taxon>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G8"/>
    <dbReference type="GeneID" id="10601009"/>
    <dbReference type="KEGG" id="aho:Ahos_1509"/>
    <dbReference type="eggNOG" id="arCOG03862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529295_0_0_2"/>
    <dbReference type="OrthoDB" id="43542at2157"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="20015" checksum="C33FD7FF86F7025E" modified="2011-06-28" version="1">MYRCCDKEFQDEKSFKKHIKSTRHHHLTSSDTFRLSLPKDYISSVIENKEGAIRLLGFLPYEDKAIIPLRLSRNVIGGVLKRSLIAKVEGPYYDTGLINYKIDAGNVKYKISFRQARLGESLRLTIIVTFEAELNFLGSILPGAVIKSLSSIMMPDDVIENVRKALLIEEKRSITP</sequence>
</entry>
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    <accession>F4B5K9</accession>
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        <submittedName>
            <fullName evidence="1">Conserved Acidianus plasmid protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">Ahos_1550</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94433.1"/>
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    </dbReference>
    <dbReference type="RefSeq" id="WP_013776348.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K9"/>
    <dbReference type="STRING" id="933801.Ahos_1550"/>
    <dbReference type="GeneID" id="10601050"/>
    <dbReference type="KEGG" id="aho:Ahos_1550"/>
    <dbReference type="HOGENOM" id="CLU_2009979_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="14331" checksum="2BC93D0B5A8BE9F1" modified="2011-06-28" version="1">MSKKLGEIIPQQPPQQTRNEKYTFDDFVKAFGDFNTFDNASHYRVMQYLKYSQTKEEQDIFSIIEKVLSLQKALAPANNNKEDEELVKNINMIFSIIKSLNDPRLIDLVKKIVEYLASRGVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5L0</accession>
    <name>F4B5L0_ACIHW</name>
    <protein>
        <submittedName>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L0"/>
    <dbReference type="STRING" id="933801.Ahos_1551"/>
    <dbReference type="GeneID" id="10601051"/>
    <dbReference type="KEGG" id="aho:Ahos_1551"/>
    <dbReference type="HOGENOM" id="CLU_1880933_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="135" mass="15000" checksum="1A3B5693BB666BAB" modified="2011-06-28" version="1">MSSKIPIKSKYSWNELNRAKEAITFFEAVIQPSLERDGISTTVIESMKISFMRDVLNIISQKYAQTHAPLDVTEAVKIIDEELPKLLKESSPGLDVNGILNMWSLVKKILSGGETENEQKVGGNNSTTTTTTNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P3</accession>
    <name>F4B5P3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated RAMP-motif protein, Csm3</fullName>
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    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0367</name>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775174.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P3"/>
    <dbReference type="STRING" id="933801.Ahos_0367"/>
    <dbReference type="GeneID" id="10599808"/>
    <dbReference type="KEGG" id="aho:Ahos_0367"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084207_1_0_2"/>
    <dbReference type="OrthoDB" id="44077at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052216">
        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF6">
        <property type="entry name" value="DUF324 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="246" mass="27825" checksum="756B393DAE890C2A" modified="2011-06-28" version="1">MGFLHSSLKYKRIVTFEIEPEEFLHIGDTKGLQIGGADLPIIKIGNKPIIPGSSLKGAIRNEFSRTVSGLSENKLKQLFGYTKLFTDNIQDILKEQDTRQIAEMIKNSLNGKDSKIGLLDLLFGSEIFASPTIFTDGLPVTQTEEYVTDRYHVRIDQDRDVAKKGNLVDVEAVYPKIKFEFKIIYNSLNYNTTDPSPVDKAFDYLVKFLDKSEILLGGWKSRGYGLVKLTKKNDETINLDNLLVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P4</accession>
    <name>F4B5P4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated RAMP-motif protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
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        <property type="protein sequence ID" value="AEE93259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
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        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
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        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF6">
        <property type="entry name" value="DUF324 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="270" mass="30089" checksum="159C5FEBF0EF55C9" modified="2011-06-28" version="1">MSNSSIYFKTYYIITKISAKITNLSPLRVGAGKGQKIGEPDLPILRTQDGYAVIPGSSLKGIVRNNLARFLMLDAEKELSYVFGGNINGEEMPIGSSLIFNDFVSNKIIDTYERSHIRINLATGGVKNLFQVEYVPEGNEFYGSILGRNISISDISGIVYMISNLLNLGIIRVGGFKSRGYGIVKLDVENVQILLPSTNVKYKTKIGIKGGEREINITVNQNEIIENNKYKFKIEKVDNNLFMNSYILNKDSFISVGKEVVKEWQATTHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5W0</accession>
    <name>F4B5W0_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5W0"/>
    <dbReference type="GeneID" id="10601076"/>
    <dbReference type="KEGG" id="aho:Ahos_1575"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="OrthoDB" id="37218at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="173" mass="20078" checksum="4F4C470333CAD9CE" modified="2011-06-28" version="1">MYEVLDYKGNPKSYIHMKIMESLVESRLALEMLKRGLLTNASSKAFISIKAFISALIVKDFDKIIQNKPEKEKEWYERIGYSAPTMGLIGVSYDLEKLGYNVSLVVRIALSLHSFSYNGFDPNLVNYKDKEEVEKDIKNVIQFVIDNAKKYFNDFWDEELEKELENLINASKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Y2</accession>
    <name>F4B5Y2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Y2"/>
    <dbReference type="KEGG" id="aho:Ahos_1599"/>
    <dbReference type="eggNOG" id="arCOG07850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_337907_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
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        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="791"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="842" mass="96015" checksum="2F400A174BC4EA7E" modified="2011-06-28" version="1">MLSNEKCFKTSQEYLIMPSTHNKHSKLGFLLLWIFSFIPISGYLIGNGYLNYYTPQFPFFSGYEVKYLYTLFVYAPETGISPSTQSVLYLFMYIIPFCNIIILAKIYLILIFSVIYVILWKLTDVILNTLNIYNFAVKILLILFFYLIPLYMQLIASDWFNVILGILSLSYVLINLINIQLKKASLTKKTVALSGIALGFSAMMDPRFYIWFIYIIIIFLLYTISTDRKSFRNIIKGLISIFVTSLPFLIYVYYIFIYPSLITVPISKAVSFAALRPDSLSYISGFSQNSDFVNLLTFISSWWPSVIPSSPSILFYPRNKWFYLPAYGFNDQILLPNDIISYIWLSSLFIFIIIVFISIFDKSRRKISSELLLALLFMIGVESGTDFPFSDFTYIFDVLPSKLPVIGGIMSTAFAIPFYAEWTNISLSLILAAIGFNYIISFFRKVKIRYIIGIVLILIMSLASWQYFNGTLYPSQRTGSFPGNSISLEGYYYPLNPPPQWVHVMNLLSTSNAGVAYVGDVGFSEKWTHYQFISYSPPLMPGYATISPPHTTNVNQAPLAYDIAGIKYLFIDNTSYNPLSNSFIYSYLNHSGLKVVYANADVYLLEQPNASVFREAKMGIYFNSSNETILFKAECLLYPLLNYTPAIITPVKTSNTVTMLLNPSIVDENNIEIFNESKNATILQGSYIIDHNGNISTFSLSGEENISIKPWTIIIPKDINIREFESIPINFSFNQFMAEFSINNNPGYLVETSLPLPYGGIIVNNGKALGVNGFGQYVFMSHGKLTISIKIGFITDLLMTIVDIFFYALFIYFFVYKNIFKGLGTEVFRNVITRISRLKSYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B639</accession>
    <name>F4B639_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B639"/>
    <dbReference type="STRING" id="933801.Ahos_0439"/>
    <dbReference type="KEGG" id="aho:Ahos_0439"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="504" mass="54923" checksum="9E62D8C2AA112C73" modified="2011-06-28" version="1">MMIIIALAYAEISSAFPESGGIIRYPHYTHGSTLGFIMAWSYIISAISTVAIEAVATTTYISHFFPSLYSGNQLTTEGIMLTYVLLVIFFLINYYGVKFLGRVSHGIGWWKIIIPTTTAVLLLAYFNPANVVFGKTACYQGLNGMLYAIPASGIVFSYLGFRQAIEYGGEGKNPQKDIPFAVISSLLISMSIFVALQLGFLGVVKTNGNWAGLLNSPLANAPLISVIDMLPVVFSFWVGILLFDAIISPAGTGIIYLGTTARSIYASAKNDYFPKLLTKINERGIPLFALIVSLISSALFLLPIPSWYQIVGISSSATVLTYIMGGIGVESMRRIAPEVKRTYVLSYINVLAPLATIFAGLLVYWSGFSTLFYIVTLVILGLSLYRRGLQGIISGIIALLNSYALYVETSALTSPSLYFIPFIIIDTAIISYLILTSAEGKFSAWAIPLLSGTLILSYISPFGLYDLMPFPYDIIVASVFFFIIHKIAVRSSLPALKIESPINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B650</accession>
    <name>F4B650_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775254.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B650"/>
    <dbReference type="STRING" id="933801.Ahos_0450"/>
    <dbReference type="GeneID" id="10599890"/>
    <dbReference type="KEGG" id="aho:Ahos_0450"/>
    <dbReference type="eggNOG" id="arCOG02462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_3_1_2"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118:SF12">
        <property type="entry name" value="CYCLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="Putative cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="203" mass="22614" checksum="EE4CA1710DA74537" modified="2011-06-28" version="1">MIIDLSVPIENGMPYFPGDPQPKIEKIEKEDYVIHSLLLSTHTGTHVDVPFHFIRDGKKLDEIDLTRFSGKAYVLDAEGKDIHSLELPSSVDILLIYTGSSKLWKNGWTMDNYATIDEEFAKLIVRKGYKLVGIDSPSIGNSKVHRILLSNEILIVENLSSNLEKIKGKVVDFISLPLPIKGVDGSPIRAIAVVRNDETHPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E4</accession>
    <name>F4B6E4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Signal-transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776479.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E4"/>
    <dbReference type="STRING" id="933801.Ahos_1687"/>
    <dbReference type="GeneID" id="10601187"/>
    <dbReference type="KEGG" id="aho:Ahos_1687"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_2_1_2"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="1">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="1">
        <location>
            <begin position="153"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="209" mass="24095" checksum="C86C181F78134116" modified="2011-06-28" version="1">MSLEYMVKNKVDKLIVNEGNKRKLVTLKGLIFGREEIFHEIDEGGTWKKEEIYDLISKYHFLFFTSSNLLIDAKDFIKSEKAKEIEVGEVMNVEPIISPPEESVYNVLQKMRERDEEYAIIVCDSLPCGILDLFGVGKAILEGRLKESVMNFAERNFFKVTPESSLETARKLMISSSRFFLPIVDFRTLLGSVSWKEVFDNLNKEAYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F7</accession>
    <name>F4B6F7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1700</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F7"/>
    <dbReference type="STRING" id="933801.Ahos_1700"/>
    <dbReference type="KEGG" id="aho:Ahos_1700"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014">
        <property type="entry name" value="MERCURIC REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014:SF4">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE RCLA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="4"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="10">
        <location>
            <begin position="326"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="443" mass="48180" checksum="D77F43962860A462" modified="2011-06-28" version="1">MGMYVVIGGGSAGYVAGSVLGREGKEVVVIEKGKFGGVCVNSGCVPSIFLSDISFLFSRLNEIGNYKGLEISVTDSKENFFSKRNEIIEYLSNAGKELVKNSGAEVIEGEAKIVSPNEVEVEGKRIAFDKLIIATGSIPLKPSIKGIERAISEDEAINLNYVPSSLVVIGGGYAGVEIAQIYARLGSEVTLITRGKIMKYLDEDGQKIIKDSLEWDGVELIENCEVEEIREHEVVTKKGMRKKGEVVVYATGRKPNFPKGIDKLGIKYNDNGIVVNDLLQTTNSRVYSAGDVIDKEKKVAHVAMLEGIISALNSMGKMERINYFSVPQVVYTDPQIGVVGDRKLAVKECKFPLSATTRAIIKGLREGYAKIGIDERGKIVYGEVVADVAEELVNELAIAIKAGMTCKDLAFTALVHPSLSESMINACRGFFNLDVDRFKDKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H0</accession>
    <name>F4B6H0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H0"/>
    <dbReference type="KEGG" id="aho:Ahos_1713"/>
    <dbReference type="eggNOG" id="arCOG02730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3038830_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664:SF2">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN, CONTAINING PIN DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="54" mass="6286" checksum="74361CCDB0451CB3" modified="2011-06-28" version="1">MIIVDTNVLVYSTFEDSENHSKALEIVEKEDVKIPQIVAYEFLWVLAKLTQMFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J6</accession>
    <name>F4B6J6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Replication factor C small subunit</fullName>
            <shortName evidence="7">RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfcS</name>
        <name evidence="9" type="ordered locus">Ahos_0518</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775322.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J6"/>
    <dbReference type="STRING" id="933801.Ahos_0518"/>
    <dbReference type="GeneID" id="10599959"/>
    <dbReference type="KEGG" id="aho:Ahos_0518"/>
    <dbReference type="eggNOG" id="arCOG00469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_1_2"/>
    <dbReference type="OrthoDB" id="7928at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000029">
        <property type="entry name" value="Replication factor C small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000129">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01509">
        <property type="entry name" value="RfcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023748">
        <property type="entry name" value="Rep_factor-C_ssu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="37"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="326" mass="37239" checksum="449F74190DB068E1" modified="2011-06-28" version="1">MEEEILWAEKYRPRSLDDIVNQKDIVERLKRFVKEKNMPHLLFAGPPGTGKTTAALALVHDLYGDSYEQFFLELNASDERGIDVIRNKVKEFARTMVSSSVPFKVILLDEADNMTADAQQALRRTMELYTESTRFILACNYLSKIIDPIQSRTALFRFYPLKKEDVVSRLEFIAKEEKVEYDEKALETIYDVTMGDMRKAINTLQAASAYGKVTIETVFKVLGLAQPKEVRDMLKLALSGKFMEAREKLRSLLVTYGLSGEDIVKQLHRELTSNELQIPEELRVLLMDYIGEVEFRIIEGADDEIQLSALLAKIAIYGNKYVGSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Q3</accession>
    <name>F4B6Q3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DEAD/H associated domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0575</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775379.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Q3"/>
    <dbReference type="STRING" id="933801.Ahos_0575"/>
    <dbReference type="GeneID" id="10600019"/>
    <dbReference type="KEGG" id="aho:Ahos_0575"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="OrthoDB" id="372104at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17922">
        <property type="entry name" value="DEXHc_LHR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18796">
        <property type="entry name" value="SF2_C_LHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010338">
        <property type="entry name" value="PRK13767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF6">
        <property type="entry name" value="LARGE HELICASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="41"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="249"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="873" mass="99723" checksum="E63BD9812DADC731" modified="2011-06-28" version="1">MSIEYAKGLSDEELFKLLRPYVADWFKKKYQTFTPPQRAAIPLIKNYENVLVSSPTGSGKTLAAFLGIIDNLFELAEKGQLEDKIYAVYISPLRALNNDMYKNLIEPLTEIKEQEKVDYNIRVGVRTSDTSSYQKEKMLKLPPHILITTPESFAISLTSPKFSEKLKNVKWVIVDEIHELASSKRGSFLSGFLEIFENMIAEDKISRIGLSATISPLEEIAKFLVGPKRSCKIVDARFVKDIDLKVISPVKDLIHATEDEISEGIYKTIIEEVLKHRTTLIFTNTRSAAERVSFKLRKLVQAQNLFDADMIGAHHSSLSRDVRLEIEDKLKRGELKAVVSSTSLELGIDIGYIDLVILLSSPKSVSRLLQRLGRAGHHIRSISKGRIIVVDRDDLVECTVLAKLAKERKIDRVYIPKNPLDVLSQLIVSASLIRPISIDEFYNIITQSYTFSELTLDSLKATVDYLTGKYGLESNKVYAKIREENGIIKPRRGSRMIFYMNSGTIPEESLVPVYTESNKYVGNLEEEFVEILSPGDIFVLGGKTYEFLSSKGNKIIVRDAEGQRPTVPSWFSEMLPLAYDSALEVGKFRKEVAEMIEKNVDKKIAIEEISRKYNISKHAAFSIYEYILEEYLFTGGKVPNDKFILIEIYDDEEDRRNFIFHAMYGRRALDALSRALAYIISEQLNVDVRISVSDNGFILTIPERTAYRISKIFDYLNPDDMYDILSKVILRTEMIKRRFRQTAERSFMILKKYKGRETNLERRQLSSEALLNVVKEIPNFPVLKETIREILEDHMDIKSSIEIVRKVKSGEIKIGVIGPRRVPSPFAHGIIAKEHADVVLAEEKRELLKKLHEEVIAYLKRKGINIDLNYTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6V9</accession>
    <name>F4B6V9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776567.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6V9"/>
    <dbReference type="STRING" id="933801.Ahos_1776"/>
    <dbReference type="GeneID" id="10601276"/>
    <dbReference type="KEGG" id="aho:Ahos_1776"/>
    <dbReference type="eggNOG" id="arCOG08511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1458125_0_0_2"/>
    <dbReference type="OrthoDB" id="43868at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="185" mass="20634" checksum="24E4CF4E61B120D6" modified="2011-06-28" version="1">MYDGILIFVIDAFLCAYKGSSTPVYTVKGIPVASGVYLQGEIKYLDVLLEKLNNNVLAQFIVFFSNGTVWCKKICKCWVYGCSPAYSAYSIIEAPSPETGVYIELPIVEGGMINFEFAYCVNGNQYAGPGTQNCFCTIANIISLSERTSCNKATVFLCNGWQRKCSGWDYLYKFVYPCGISTYWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X3</accession>
    <name>F4B6X3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartyl protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776581.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X3"/>
    <dbReference type="GeneID" id="10601290"/>
    <dbReference type="KEGG" id="aho:Ahos_1790"/>
    <dbReference type="eggNOG" id="arCOG03743">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145188_0_0_2"/>
    <dbReference type="OrthoDB" id="28066at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="2">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="13197" checksum="0FA174F3B510AED1" modified="2011-06-28" version="1">MGLIYVDGIVKNEKGDSEKVRFLIDSGAYYTVLKKEIWEKLGLKEISKISLVLADGTTIERGVSEAIIELPPYGERHSPVILGESEDENLLGVVTLEIFGLVLDPLKREIRQGRIIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X4</accession>
    <name>F4B6X4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH iclR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776582.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X4"/>
    <dbReference type="GeneID" id="71811312"/>
    <dbReference type="KEGG" id="aho:Ahos_1791"/>
    <dbReference type="eggNOG" id="arCOG00393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269849_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="HTH iclR-type" evidence="2">
        <location>
            <begin position="56"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="25269" checksum="4316AD6387187B58" modified="2011-06-28" version="1">MHTSSFSPAFFILAIVLSDSSLLFFFLYYIRSNKRTKEEEKEEEIQIGLDERDKVVLDALRKSGGTSTLSELVKQTNLPKTTVYRRVKRLVAMGYVEEVREKGKVRYVLKKDYVEKMITHIKIILQAIYQNFSALSRKGLAKEILSTTPSKVSYNSIVKEIGVNHRTVEECIKLFNDMFLTLTLHFIDVNIGYFNYGKQIKIHFIDPLFYDVVSFWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y8</accession>
    <name>F4B6Y8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776596.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y8"/>
    <dbReference type="STRING" id="933801.Ahos_1806"/>
    <dbReference type="GeneID" id="10601306"/>
    <dbReference type="KEGG" id="aho:Ahos_1806"/>
    <dbReference type="eggNOG" id="arCOG03707">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461589_0_0_2"/>
    <dbReference type="OrthoDB" id="43833at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10286" checksum="1E6FBA8249DEE4BF" modified="2011-06-28" version="1">MEVTKPWIDDNKYKKDRLLEAKYEAELAKKFLEDGLYRNDTGKAFQAWKALLASLSVDYIQEIPSLDFAKMDLEKLLKEIEKYLVGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z8</accession>
    <name>F4B6Z8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="10">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gltX</name>
        <name evidence="15" type="ordered locus">Ahos_0593</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775397.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z8"/>
    <dbReference type="STRING" id="933801.Ahos_0593"/>
    <dbReference type="GeneID" id="10600038"/>
    <dbReference type="KEGG" id="aho:Ahos_0593"/>
    <dbReference type="eggNOG" id="arCOG04302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_3_2"/>
    <dbReference type="OrthoDB" id="10470at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000222">
        <property type="entry name" value="Glutamate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003169">
        <property type="entry name" value="PRK04156.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="98"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="13">
        <location>
            <begin position="411"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="14">
        <location>
            <begin position="500"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE93481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="570" mass="66062" checksum="467029568552E3C3" modified="2011-06-28" version="1">MEEIDEIIYKYALDNAVKHDGKAAVGPVMSKVLGEKPELKPKAKEIAKRVQEIVEKVNSMSLEDQKKELEAKFPEMLTKKKTEEEKKTLPPLPNVKGEVITRFAPNPDGPIHLGNTRAAILSYEYARMYKGKFILRFDDTDPKVKKPLREAYDWIREDLKWLGITWQEEFTASSRLERYYEVAKEMIEKGYAYVDTCSEEEFKKMKETRNFSLPCFNRSKPVEYNLELWEKMLERKFKEGEAVLRIKTDLNDPDPSKIDWVMARIVDTEKNPHPLVGDKYWVWPTYNFASAVDDHDYKITHILRAKEHMSNADKQKWVFNYMGWEIPEVMEFGRLKLEGFMMSKSKIKGMMEKGTTKDDPRLPTISGLRRRGILPETIREIIIEVGVKPSDATISFENIAALNRKKLDPIAKRIMYVENYSEFTLEIPGEMTAKIPLSPSMKEYREITVKPGDKILVEKADAENQSIIRLVELCNVKVEGNKLVYVSKSVEDAKKMNAKIVQWVKKGEEVNVNVIKADPNQDLKIINGKAERYAINLNPDEIVQFIRYGFVRVDSKNESNNELTVIYAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B709</accession>
    <name>F4B709_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF460 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="607" mass="69704" checksum="FE0DCB14E9E9CE5B" modified="2011-06-28" version="1">MGIDINPGSSPANLLDAKYSVVILDENGNIVQKYDEIGISKIIRLAWEYEVSIVATDNIYELGENDRQVIKIISLFPDNVELVQVTYNDGRFMDIREVAKNMGIEIQGKPNPSKTAYLAAYLALKGAGTKIKLTENKTKIIISRGRHLGPGGMSSNRYKRHIRGLILRVFKEVKETLDRNNIDYDVILRRSKSGLENATFIVYAPRERLYGLVKKMSGHDVNLEIRPVYKTKIDFETNKKIEKKPIIVGIDPGIFVGISAIDIHGNPVILESKKGIDREEIINKLSEKGSAIIIASDVNPVPDAVKKIAGILKAKLYVPDKSLSVDEKQKLLQEYSEKHNLNITDPHIRDSLAAALKAYKELENKLSQAFSTIRKLDLDIDEYAIYKCIIEGKTVAECIEKQIENEIAEEENKEIIKVEKTEVKEKQPENKTFTENSELKHEIFRLRRTITRLLNEKYELEKKLYEIRTQFNAEVERDRRLYRLKTELEERNKSILKLQEIIKDYSDKINKLEGIIDELVEGKVNIIKSNSLIEISDYKVRILGEEVNPSIFNYVGKDFIICKSNILDDIRKLSKEKEIQKEMDINDIKNIIEEYRRQKSKHGNFAI</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="GeneID" id="10600080"/>
    <dbReference type="KEGG" id="aho:Ahos_0633"/>
    <dbReference type="eggNOG" id="arCOG01761">
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    <dbReference type="HOGENOM" id="CLU_132460_1_0_2"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0003746">
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    </dbReference>
    <dbReference type="NCBIfam" id="NF005013">
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    <evidence type="ECO:0000313" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="168" mass="18923" checksum="F75106097D8B0806" modified="2011-06-28" version="1">MKIPLDYLCVKSGLLCNRCQSLVDSGEVDSFEVKVMQALLELEETQFKELKDSTYYKAIKVNDLLILLVGSGPSMTIQKWIKVAKALQDKLHMKVRIVEKTNNIKNSAIQLLSPARVLGVNTVWLPDGSVQYVIRVSKSERKLLPADSNILESALSKIHDTRVKIRVE</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="entry name" value="Metallo-depent_PP-like"/>
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AEE93533.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18489" checksum="C809EEA1432A8C63" modified="2011-06-28" version="1">MKKLAMITRFPCQEDAVEYINNNFDGVIGLGDVECPQYLNNFHGILGEMESVYIMKYLKKTERLITKEFLGLSTDFSTEISITHFPPKGFKSGIIINVEIGKKEISAKILSSKTKIVIHGHSENQTIVNSHGFQVISIGAFEKGYLAIYYPEEKKIQLSKISSH</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <property type="entry name" value="ORF_D-335"/>
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            <begin position="14"/>
            <end position="49"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
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            <dbReference type="Pfam" id="PF07935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93536.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="49" mass="5789" checksum="6290F5D588AC87C2" modified="2011-06-28" version="1">MTSRGHGLNMGYRVFSAGQYKIRQRGKKYYVYLIEKHKDGNLRERTLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B760</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
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    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS2 family.</text>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0656"/>
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    </dbReference>
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        <property type="term" value="P:translation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000030">
        <property type="entry name" value="30S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_uS2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_uS2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="226" mass="25306" checksum="D18442EE84A3F5C7" modified="2011-06-28" version="1">MSEEENKGTELSEAEKAELQKSEKGAVIELLAPLENYLSAGVHIGTHTCTKYMEKFIYRVRPEGLYVLDVRKIDERLRIAAKFLSRFTPSGILAVASRPYAFTPVQKFAEVVGGRAITGRLVPGTLTNAYLDRFIEPEVLLVSDPRTDTQAIKEAADMGIPVVAFADTDAKIEYVDLVIPANNKGRKSLALLYWLLARQILRERKQIPMDADIPVKVEEFEVKLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B775</accession>
    <name>F4B775_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IS607 family transposase protein TnpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1820</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776610.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B775"/>
    <dbReference type="STRING" id="933801.Ahos_1820"/>
    <dbReference type="GeneID" id="10601320"/>
    <dbReference type="KEGG" id="aho:Ahos_1820"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="OrthoDB" id="41819at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF22">
        <property type="entry name" value="TNPB-LIKE PROTEIN MJ1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="Probable transposase IS891/IS1136/IS1341" evidence="6">
        <location>
            <begin position="191"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Cas12f1-like TNB" evidence="7">
        <location>
            <begin position="311"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="427" mass="50502" checksum="65C3DBAFB323C9FE" modified="2011-06-28" version="1">MSKSLRLKSFQPEEEYVYLTYSLKNDKKEESKILLENYRVLLQKALDWLWEKVERKEVKGKKATKIKITLPKKKEVYKVLRDELEKINNLASHYVDKAINDAYSILGSWRRRAEKGQASLRKPTLRKFYVRVKSTLRKVEGEEVRITVRPYEYITFSWSHIWFSRRVKGLELGEPIIKEDKVYLPFRYRLPWFTPLDFLSIDSNLYTLDAYDGEKFVTFTIKESYSLKFGMELKRGRIQSFASKHGRKGKVLLRKYSHRERNRVLDYVHKFVNKLLEMYPLTIFAVEKLNKQSMFQDADDKLSKKVSKTVWRTIHRVLKYKAPLYGSFVKEVNPHLTSKSCPRCGWVSRKVGRTFHCERCGFTLDRQLNASLNIYLKMCGFPHILGIPRLWVGVIPLKGRRGMSRAMPRDPGEAQGLRIGYNFMKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B786</accession>
    <name>F4B786_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776621.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B786"/>
    <dbReference type="GeneID" id="10601331"/>
    <dbReference type="KEGG" id="aho:Ahos_1831"/>
    <dbReference type="eggNOG" id="arCOG12101">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1044273_0_0_2"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="30930" checksum="3478849953B8856A" modified="2011-06-28" version="1">MRKAIYFDNRLGISSFLNNEILIEKPVDYEVLDYYTISKWLEQSDKDDILIFAQDIVPYTAYEITPFNNDSKLLNFIRRGGIVTWIGDVPFFYRLHCFSKEYKEKIERVKDLLKKQVAFTPTPEFYLQKFGPYDKGDQICVLDIIGGFYVDVDRENVNWLSLDYEHLGFFKLNEVCYLYSPSAVNKTLLGKLLQYESNASLRPIKYTTKILPLSLTTLSGVCKGKYAGSWIAQIGEGYFIRLYDVKEELNAHKVFEITEKIASTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B791</accession>
    <name>F4B791_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="3 12">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">uppP</name>
        <name evidence="13" type="ordered locus">Ahos_1836</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="3 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B791"/>
    <dbReference type="STRING" id="933801.Ahos_1836"/>
    <dbReference type="KEGG" id="aho:Ahos_1836"/>
    <dbReference type="eggNOG" id="arCOG04761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060296_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001398">
        <property type="entry name" value="PRK00281.3-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622:SF2">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010621"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00021581"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="29146" checksum="D62F344C33BD2A49" modified="2011-06-28" version="1">MVKMNFLVSILLGIIQGISEWLPISSKTQELIASHYLLGLDVSIAYTFGLFMEMGSIGSALIYFRQDVKRVFHDKFLLKFLVVVTAFTGIVGVPLYVISDKLLQNAYNPSIPMIFLGIALIADGIYIKYSRSRTREFKNLSTKEMILIGIAQGIAALPGVSRSGMTVSTMLVLGINPEDAFHYSYLAYIPAAIGAVGTTLLFTRHHVSYVISMIGIDGIALAVISALLTGLVVIGFLLKIAKTKKVYLIDFMLGGIAVLVSMLGLIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B0</accession>
    <name>F4B7B0_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="AlphaFoldDB" id="F4B7B0"/>
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    <dbReference type="GeneID" id="10601356"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_369450_0_0_2"/>
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    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="753" mass="81605" checksum="642DF4790EE1BBE3" modified="2011-06-28" version="1">MVEKKVSNKRSKGISLVIAFVVLAIIIFSVLIPFIIIRSTTPNYSNISAVSASALSSERELQVQEVESGDPSISAFQGSQGQVYLCFHYNHGSTPITIIGVYYFCQNTGKWISAYPKPFTVTGNERIYLFTPIGYTGLIEIVTNLGNIFYAPVSTIGAAFSIPLYKITQTTSTPSYVTTPCHKTIILPNGTEIITRCCVLPKLAQSNSCYKLPNGTVLVLPCGKVCRLPNGGILHLNNNLLKQEYINSAYFPFSSFLTGYQSDNDNIGPLPVGQRNPELNTQTIVQWFIASGNLAPICIYVCSNAPSTAQENTVYISKYAPTGGYIVWTGDAGLIVGAGKIGLVDSPHQPSGPQSPPYYVAFTVHLANGSWATIVDTTPIPTVGKENGQPASPLTLAVGVFNSKTGTLCLYINGTLVSKATVNCKKLCVNPLSYFDVASAYNGGGSPWLCELPGFPGDMYTGGNNLYSFNGALGPTIIYNTSLTPHQIKSLLVGCIPSPDNIVILWSQNSAEYVHICTSTWTVTPCYKYCSNPQGGPAGKCPYGEYDYEVFNLANLCQFNGFWGLAGAIPTSFAPFSDVVLWGGASPIVLNPSVLVHCNVNITVRYSACLIPTTPGGYIFSISFTDPLPGATVERNVQTVTYANEWATVYINGQKVFEGKFVNNQLYVLYSNDPCYQQKNIPEFGCYLKSPVNLTVIFTFNMYEGGANNQPGGPAGPQSSCNYPVVYFNIMWLQPGAQWLTYIPITQLEPYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B1</accession>
    <name>F4B7B1_ACIHW</name>
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        <submittedName>
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    </protein>
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        <lineage>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776646.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7B1"/>
    <dbReference type="STRING" id="933801.Ahos_1858"/>
    <dbReference type="GeneID" id="10601357"/>
    <dbReference type="KEGG" id="aho:Ahos_1858"/>
    <dbReference type="eggNOG" id="arCOG03855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478902_0_0_2"/>
    <dbReference type="OrthoDB" id="34305at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="177" mass="19008" checksum="303640011360EBA8" modified="2011-06-28" version="1">MDIALSDFILIIAAVIVGLVIFGFTTAYIAPQYAFTYAEQSAKTISQTTFVSISPPETTNSGCCNYLVDVYSPGFSGNFTVVAFYIPSSELQSIAIVTPSSSSSFPVYINSTLASKVTLSNVYDINGHLLVSSISGYTIPANYPIQIIAPPKPGCSLVIWIIYSNGGYNFRVLYAYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B4</accession>
    <name>F4B7B4_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7B4"/>
    <dbReference type="STRING" id="933801.Ahos_1861"/>
    <dbReference type="GeneID" id="10601360"/>
    <dbReference type="KEGG" id="aho:Ahos_1861"/>
    <dbReference type="eggNOG" id="arCOG06002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691446_0_0_2"/>
    <dbReference type="OrthoDB" id="375253at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="155" mass="17435" checksum="42F472CFCC1EDE27" modified="2011-06-28" version="1">MKGLSNVIMLVIILIIVLSVFIPLGIEFMYFPQNNQEILFSKESYNYYHCLICQDIALGFLKISYLTQKVGGVYKVILTFCLAKGVGGIPICKINITEILYYNQGHWCVVKLSYPIIISISYSPHSITFYLNVLSDLAIVTNYGNLIFIAPCSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C1</accession>
    <name>F4B7C1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1868</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
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        <property type="entry name" value="AA/rel_permease1"/>
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        <property type="entry name" value="AA_Transporter_AGT"/>
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    </dbReference>
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        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
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    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
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        <property type="entry name" value="AA_transporter"/>
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        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
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            <end position="69"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
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            <end position="197"/>
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            <end position="274"/>
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            <begin position="441"/>
            <end position="463"/>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="529"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="572" mass="60503" checksum="6713BF5AB8DF1AF6" modified="2011-06-28" version="1">MSRQNVTSSGVKLKRELRLIDLYFSGLTAVIGSGWIFGSLETASVAGPAAILSWLFGGIFILIIALVWAEVGTSIPVAGGAVRYAKYSHGGLAASIISWSVILTYIAVPSAEAVAVVEVLQGALSLYGITSVTLCSSGFPTAEGLGVALALSALFFFINYIGISALRASNFAITSWKLIIPSLTVIAILIVGVPRFASNFTTPSFAPYGFLPVFSAIPATGVAFAYLGFRQPIEMAAEAKMPGRDIWRAILAVVGTSIILYTMLQVVFIGGFTWNSSAFGTTGVSPGDWSALSTSPYPPIYSFPFFYEVAGLGLGVLAILLAIDGVVSPSGTLSQYMASTARVLYGTSKEGFLSDKFFEVHGKYRVPVWGLIATMIVTIALLVMAFAGTLVSSVGGAWSALVSIITTTGVFSYIIGSVFLPVSRKYAPDLPRPFKLPAYQAFSLAAFIISALLVYWGAGALIAPPSDPYGGYILIAVMLAGALTYLTYKDKKPCDLKSGLWVVAFLIFTGVLVALGCYGFGIIPSSPLPFAWIIDIIAAIGFYFWAINSAVPECHVKVNIDKGIEASNNESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D0</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Selenophosphate synthase-like protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">Ahos_1877</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="KEGG" id="aho:Ahos_1877"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="460" mass="51863" checksum="A1D5792FF87247FF" modified="2011-06-28" version="1">MEEVFERFRKNLEVYNKMGLNPLSLATGCAVKVDLIDTVYPALEKIKNKLENIEILPREDADIFITREKMEIKRIIGGGNFDADRGVSLIQVNQDTAGNPEKFANFLLKTYSSIRSRRKLTIGKGHSIVTTIPSAEVAVLDLIKLDGSKINSYTLANNDTIQIVDPLEDPGSQMQVDVAISNSLNDLFTKGAYQNLKMLPLFDAPNDDLKERIMKNYENYSEKYDIPIINDIQPNTKSLMLGATVLGESDHELPTYYDKVEEGDEIIVTRPIGELAPINVYLWILVVPEVLELMESRGISFKRLEKVKMEVIEQMRKPNIPTAKVIYNYLPEFGKSFDRENYITMTTDVTGPGIFVIKEFAEKAGVDVRLDKIPVIDEEIAEFATEGFIIPNSTSGTNGATVVFYSRRISDDIIEDLQKEGLKPIKIGKVLGKGQGVVHVNKDIERFIHRKNVLSHFKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7E1</accession>
    <name>F4B7E1_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1888</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7E1"/>
    <dbReference type="STRING" id="933801.Ahos_1888"/>
    <dbReference type="GeneID" id="10601387"/>
    <dbReference type="KEGG" id="aho:Ahos_1888"/>
    <dbReference type="eggNOG" id="arCOG07291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2217100_0_0_2"/>
    <dbReference type="OrthoDB" id="36437at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="12772" checksum="668226088C22D2F3" modified="2011-06-28" version="1">MMEEKEVVLKALEKVDKWYVQLAGIKGDELLIVSQKEVPKEIEVEGRKLTVKHYSPEEYLTVITKDENEFRSYHVYYFVKMYMRKVLDLLAYLEVNRIRIDERDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F0</accession>
    <name>F4B7F0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="8">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tyrS</name>
        <name evidence="10" type="ordered locus">Ahos_0668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F0"/>
    <dbReference type="STRING" id="933801.Ahos_0668"/>
    <dbReference type="KEGG" id="aho:Ahos_0668"/>
    <dbReference type="eggNOG" id="arCOG01886">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035267_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02009">
        <property type="entry name" value="Tyr_tRNA_synth_type4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023678">
        <property type="entry name" value="Tyr-tRNA-ligase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="344" mass="39180" checksum="09FC6A42600D38B4" modified="2011-06-28" version="1">MEEIVTLDDLKKKLENNEKLTGYIGFEPSGLFHIGWLIWAQKVKDLSNAGVSMTLLMATWHAWINDKLNGDMELIKLAGKYAIDVLSAYGLDMSKINVVDAEDIVKDKAYWELVLKVAKNTTLARMKRALTIMGRKSDEAELDTSKLFYPAMQVSDIFYLDVDIALGGTDQRKAHMLAREIAEKFHRKKVIAIHTPLLVGLKGGQRMGNVEEDDLLSEIKMSKSKPENAIFINDSPEEVTAKIMRAYCPSRVVELNPILQINRYIIFTNVNKLKVERDIKYGGDIEFNNYEELEKAFAEGKLHPMDLKMATARKLNEILEPIRKKLNTPEYEELVSKISKNVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I1</accession>
    <name>F4B7I1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0699</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775502.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7I1"/>
    <dbReference type="GeneID" id="10600151"/>
    <dbReference type="KEGG" id="aho:Ahos_0699"/>
    <dbReference type="eggNOG" id="arCOG05941">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143937_1_0_2"/>
    <dbReference type="OrthoDB" id="46187at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="107" mass="13048" checksum="DB69FEB65A36E8AD" modified="2011-06-28" version="1">MLVRLEEKEFDKIFSKLKEQVYEYNSKISGSEVYLKPYHVVYKNGKKYIYIGKYWYKLEKYNGKIKWIYLGKEKPLANLPDPPRFPEFTIIKDEKSYIVDEKFLNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J7</accession>
    <name>F4B7J7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="5">4.99.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">CbiXS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiX</name>
        <name evidence="6" type="ordered locus">Ahos_0715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+)</text>
            <dbReference type="Rhea" id="RHEA:15893"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60049"/>
            <dbReference type="EC" id="4.99.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CbiX family. CbiXS subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.3" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775518.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J7"/>
    <dbReference type="STRING" id="933801.Ahos_0715"/>
    <dbReference type="GeneID" id="10600169"/>
    <dbReference type="KEGG" id="aho:Ahos_0715"/>
    <dbReference type="eggNOG" id="arCOG02246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065901_2_1_2"/>
    <dbReference type="OrthoDB" id="11653at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03416">
        <property type="entry name" value="CbiX_SirB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00785">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023652">
        <property type="entry name" value="SiroHydchlorin_Cochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050963">
        <property type="entry name" value="Sirohydro_Cobaltochel/CbiX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542:SF3">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="68"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14339" checksum="ACAFA077D16E9AC6" modified="2011-06-28" version="1">MIGIILVLHGSRVEEWKNIADGYKNLLSEYFPLVEYGFLEFNKPTLRESLEILVNKGATEIIAVPLLFAAGMHFYRDIPKLLGLDEKGETMINGKKVRVVIAKPIGVDKRVAEILKERVEEKIDGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7K8</accession>
    <name>F4B7K8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="3 7 8">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribH</name>
        <name evidence="9" type="ordered locus">Ahos_0726</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="3 7 8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775529.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7K8"/>
    <dbReference type="STRING" id="933801.Ahos_0726"/>
    <dbReference type="GeneID" id="10600180"/>
    <dbReference type="KEGG" id="aho:Ahos_0726"/>
    <dbReference type="eggNOG" id="arCOG01323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_3_1_2"/>
    <dbReference type="OrthoDB" id="7610at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Lumazine_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LS"/>
    </dbReference>
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        <property type="entry name" value="LS/RS"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
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    </dbReference>
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        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="47"/>
            <end position="49"/>
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        <ligand>
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            <end position="72"/>
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        <ligand>
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            <end position="76"/>
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        <ligand>
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            <position position="103"/>
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            <position position="118"/>
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        <ligand>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="16894" checksum="A00AFE3B8BDEFF05" modified="2011-06-28" version="1">MQDQSIKLGLVVAEFNYDITYLMLQRAISHAEFLGAKVSVIFKVPGSFEIPLAVKKLIKLDVDCIAVLGAIIKGETKHDELIANQIARLISDLEVEYEKPIGLGIIGPGATHEQAVERIEEYATRAVEAAIKMAKRLRKIPEKLNEEKVIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P2</accession>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P2"/>
    <dbReference type="GeneID" id="10601415"/>
    <dbReference type="KEGG" id="aho:Ahos_1916"/>
    <dbReference type="HOGENOM" id="CLU_2152463_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="111" mass="12886" checksum="755943238BD96F0F" modified="2011-06-28" version="1">MDVELLLYSEFLRNKEKMLRTLKEKFNNIAGIGFISNSKSQTLKYKEKFSMHGYKIENPMLINIIKLNPETEVPEAYEILKRLKVSEDNSISFSISFPKVETLYAAIFNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q2</accession>
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        <submittedName>
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    </protein>
    <gene>
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    <organism evidence="1 2">
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        <lineage>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Q2"/>
    <dbReference type="STRING" id="933801.Ahos_1926"/>
    <dbReference type="KEGG" id="aho:Ahos_1926"/>
    <dbReference type="eggNOG" id="arCOG06019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574881_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="21635" checksum="54AA6FAC8DFFB3CA" modified="2011-06-28" version="1">MIDELFLMYKLVNDEWSKAVSELFQLLSTSLRNRFAMLIALDEDSFVYDSNVLVVVDKVDDEVRDKVAEAVIEVNSHHDCTISYYLTSKDDVNTIRVFSSVKEKKKEDCEDAFKEFKEKVKSIARDVIKAEIYDSNVLVVVDKVDDEVRDKVAEAVIEVNSHHDCTISYYLLSSEEYEKIRGTIKGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V1</accession>
    <name>F4B7V1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V1"/>
    <dbReference type="STRING" id="933801.Ahos_0742"/>
    <dbReference type="KEGG" id="aho:Ahos_0742"/>
    <dbReference type="eggNOG" id="arCOG01759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131318_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="entry name" value="Bifunctional nuclease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="BFN_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="Hypothetical protein TM0160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="1">
        <location>
            <begin position="31"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="183" mass="21186" checksum="5A064BA759FFB034" modified="2011-06-28" version="1">MISGIYNYLLMKGLICCKSYLFLIMKEEEDYIKVNRVDAFVYPLDGLPVMVCYLEDGREFNLFYVPIEIVIAINKIKKQYEEDSILGDKRETIFDILSFIPEVTEEISKHINKVTIDDISGSVYIATIELKFDGVIIQKRMIPSHAIYLALISNKPIYVKKSLVDEQEKERKKGEGGEEEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V6</accession>
    <name>F4B7V6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit A'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rpo1N</name>
        <name evidence="13" type="synonym">rpoA1</name>
        <name evidence="17" type="ordered locus">Ahos_0747</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds at least 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775549.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V6"/>
    <dbReference type="STRING" id="933801.Ahos_0747"/>
    <dbReference type="GeneID" id="10600204"/>
    <dbReference type="KEGG" id="aho:Ahos_0747"/>
    <dbReference type="eggNOG" id="arCOG04257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000487_3_1_2"/>
    <dbReference type="OrthoDB" id="371812at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02582">
        <property type="entry name" value="RNAP_archeal_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00863">
        <property type="entry name" value="RNApol_arch_Rpo1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012758">
        <property type="entry name" value="RPO1N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02390">
        <property type="entry name" value="RNA_pol_rpoA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 13 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="16">
        <location>
            <begin position="205"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE93633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="880" mass="99667" checksum="E3267BFDB17862B0" modified="2011-06-28" version="1">MSEKIIKGVKFGILSPDEIRKMSVTAIITSEVYDEDGTPIEGSVMDPRLGVIEPGQRCPTCGNMMGNCPGHFGHIELVKPVIHVGFVKHIYDLLRATCRRCGRIKLEDQELERYRRIYHAINKRWPSAAKRLVEHIKKSSMKAPVCPHCGEKQLKIKLEKPYNFYEERKEGVVRLTPVDIRDRLERIPDDDVELLGYDPKTSRPEWMILTVLPVPPITIRPSIMIENGIRAEDDLTHKLVDIVRINERLKESIDAGAPQLIVEDLWDLLQYHVATYFDNEIPGLPTSKHRSGRPLRTLAQRLKGKEGRFRGNLSGKRVDFSSRTVISPDPNISIDEVGVPIDIAKILTVPERVTKWNIERMREYVLNGPDKWPGANYVIKPDGRRIDLRYVKDRKEFASTLAPGFIVERHLIDGDIVIFNRQPSLHRISMMAHKVKVLPGRTFRLNLLVCPPYNADFDGDEMNLHVPQSEEAIAEAKELMTVHKNILTPRYGGPIMGSAQDYISGAYLLTVKTTLLTEDEVQTILGVANISKDIGEPAILAPKRLYTGKQVVSLFLPEDFNYHGQANIASGPRLCTDEDCPHDSYIVIKNGKLLEGVFDKKALGNQQPESILHWLIREYPEDYGLWLMDNIFKVFIRYIEIHGLTMSIDDVTISQEAMAKIAEKAKQADKQVQELIAKYEKGELEQIPGRTLEESLESYILDTLDKLRNDAGEIAAADLDPFNNVYIMARTGARGSVLNITQMAAMLGQQSVRGERISRGYYERALPHFKRNDISAEARGFIYSSFRSGLNPIELFFHAAAGREGLVDTAVRTSQSGYMQRRLINALSDLRIEYDGTVRTLYGEVIQTLYGGDGVHPMYSSHGKTVDVNRILERVVGWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W4</accession>
    <name>F4B7W4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11 12">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 11 12">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">upp</name>
        <name evidence="14" type="ordered locus">Ahos_0755</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="3 11 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775557.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W4"/>
    <dbReference type="STRING" id="933801.Ahos_0755"/>
    <dbReference type="GeneID" id="10600212"/>
    <dbReference type="KEGG" id="aho:Ahos_0755"/>
    <dbReference type="eggNOG" id="arCOG04128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_2_0_2"/>
    <dbReference type="OrthoDB" id="80352at2157"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_A">
        <property type="entry name" value="Upp_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034331">
        <property type="entry name" value="Upp_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="UPRT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="13">
        <location>
            <begin position="7"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="30"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="140"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="216" mass="24381" checksum="08CFAA69A133BFCF" modified="2011-06-28" version="1">MPLFLLSSPLSLHILTQIRNKDTDQINFRKGLVRLGRLIGYEIANTMDYEIIEVETPLGVKTKGVRIYDLENVIIINILRAATPLVEGLLKAFPAARQGVVTARRKEFEGKEPPKEMEVEIFYEKIPKIRKGIDNVIISDPMVATASTMLKVLEKVEKEEPKRIYIASVIASEYGVKRILELYPQVYFFTISIDPELNNKGYIIPGLGDAGDRSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W8</accession>
    <name>F4B7W8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-dependent hydrolase, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775561.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W8"/>
    <dbReference type="STRING" id="933801.Ahos_0759"/>
    <dbReference type="GeneID" id="10600216"/>
    <dbReference type="KEGG" id="aho:Ahos_0759"/>
    <dbReference type="eggNOG" id="arCOG05932">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131312_0_0_2"/>
    <dbReference type="OrthoDB" id="35660at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="167" mass="18740" checksum="635E958F13C72246" modified="2011-06-28" version="1">MTFAYLGNGVYTIDNEMYIINTDENNFFIIDSKENIGNSELMDILEITKGKKPMYLFLTSCCKSVSGGAWYIYQTLGTRIIAHYPDSINIRHGECNNLTYTPVNISIELKGYINYVENIKVISSKVPTSGSVIIKWKGFLFVGNNKTISPYGNEVKYVCGVNGCIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X8</accession>
    <name>F4B7X8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prephenate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0769</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X8"/>
    <dbReference type="STRING" id="933801.Ahos_0769"/>
    <dbReference type="KEGG" id="aho:Ahos_0769"/>
    <dbReference type="eggNOG" id="arCOG00255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_0_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="149"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="224" mass="25701" checksum="23E0CA5B00324B9E" modified="2011-06-28" version="1">MSSEGKLGIVPIENSLEGPVNETLDNLFKFDDIFVNKIIELKINLVLASKEDINIKHIKNIYTHLYAYKEAQNSLRKLGITNNIIPVESTSKAALFALSDPNSAALCSEFAARLYGLKILAYRVQDYDNNITKFALISKNVSFRGERSMILFTVPDLPGSLYKVLEKFYVFNKNLKMIYSRPIRSIPWNYYFYAEYEGSYEEKLLNELKKVTTSLKFKGSYNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z9</accession>
    <name>F4B7Z9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z9"/>
    <dbReference type="STRING" id="933801.Ahos_0790"/>
    <dbReference type="KEGG" id="aho:Ahos_0790"/>
    <dbReference type="eggNOG" id="arCOG00187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001546">
        <property type="entry name" value="RNase L inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="75"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="331"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="600" mass="68642" checksum="0C9E65DA9E23D787" modified="2011-06-28" version="1">MRVAVINYDFCKPDKCNLECIRFCPINRSGSKAIELSEIVKGKPVIYEETCIGCGICVKKCPYEAISIVNLPDKLSGEIIHRYKINGFELFGLPILKQGYIIGILGKNGTGKSTILRILSGELIPNFGDPNAKLSYDDVLNKFKGKEIYDYFYKLYNKNLKVVHKIQYIEYAGRMLKGNVNELLNKIDERGKIDEVRELLSMQNMWNKSVQTLSGGELQKFLVAAALLREADVYVFDEPSSYLDVRERLNMAKGIRELTKDKYVAVVDHDLIVLDYLTDLVSIIYGESSVYGRVSKLYSARTGINNFLNGYLPAENVKFRQDEIKFMLKELTDLDFSKNVKIRIKWSEIKKNLSDFSLDIEEGDAREGEVIGIVGPNGIGKTTFMRILVNEITPDYGYVMPEGLRMSYKPQKLIPEYDGTVQQYLENVSKDILSTSSWFFEEVIRKLNLHRILESEVKDLSGGELQKLYVAGALAKEADIYVLDEPSSYLDVEERYIVAKAIKRITRERKAVTFLVDHDLAIHDYISDRIMVFTGIPGREGHGKSPTSLRKGMNEFLKEMQITFRRDADTGRPRVNKLGSYLDRLQKERNEYYSLEVSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B812</accession>
    <name>F4B812_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="6">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tgtA</name>
        <name evidence="8" type="ordered locus">Ahos_0803</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B812"/>
    <dbReference type="STRING" id="933801.Ahos_0803"/>
    <dbReference type="KEGG" id="aho:Ahos_0803"/>
    <dbReference type="eggNOG" id="arCOG00989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="7">
        <location>
            <begin position="14"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="492" mass="56698" checksum="53692C2D337D7425" modified="2011-06-28" version="1">MIGDFEVKEEDLAGRIGKLETSHGILETPAFFPVVNVLRKEISIKDIMAVGFNSIITNAYILKKNNIVKQDIHKELDFNSIIMTDSGAYQILEYGNIEANNREIVEYQNQIKPDIAVILDVPTGKLEDREEAKMTVDETLRRAKDVESIINTENIIWTHPIQGGRFLDLLQYSAVEANKREKYKLLALGSPTVLMEKYDYDTLIDMIFTVRSSISRGKPLHLFGGGLPHLIPFAVALGVDSFDSASYILYARDNRYITRSRTLKLEEIDSFPCSCPVCSRYTPQELLEMDESERTRLLAIHNLYKILEEIRETKIAIKEGRLFEYLQEKAFSHPSVYSAFERLLKYAEYLEKYDPRVKGEIKGLFLYDENSIKRPEIIRHYRYLDLIKPMHDKAIVICGDKFAQPFILDPFILKIVKEKYMEADIYIAYPFYGLIPVLVSESYPLSQFEIPKSLSESVIRISSDKANSILKAKYKKVELIECENSKLHIMPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B828</accession>
    <name>F4B828_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776767.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B828"/>
    <dbReference type="STRING" id="933801.Ahos_1980"/>
    <dbReference type="GeneID" id="10601479"/>
    <dbReference type="KEGG" id="aho:Ahos_1980"/>
    <dbReference type="eggNOG" id="arCOG07329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076113_0_0_2"/>
    <dbReference type="OrthoDB" id="34422at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="242" mass="27373" checksum="0A400007A21F641F" modified="2011-06-28" version="1">MEDLFLLSIIAGVMITAIVFLLHEAERVKSKLGFSLVVFGFVMMITMFLGASFYLLSPSNLSLAEAILINNFTMIAYILLVFPFIKKFKVKFEMSYISSLSVSLLAVINEILMSLTFNIACGVSNLFYFTFNSGWFFYPMMAEMLSIYLIHYIKGEFRKDLFPLIGITSFPPTLIDNAKWFYSSLAISLALSGLGVINSKGFIRGIYVALILSLLLLFKVYIIYDVVITVSMITYYFSLLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B886</accession>
    <name>F4B886_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Arylmalonate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776825.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B886"/>
    <dbReference type="STRING" id="933801.Ahos_2039"/>
    <dbReference type="GeneID" id="10601539"/>
    <dbReference type="KEGG" id="aho:Ahos_2039"/>
    <dbReference type="eggNOG" id="arCOG02004">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068086_5_1_2"/>
    <dbReference type="OrthoDB" id="41425at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1860">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026286">
        <property type="entry name" value="MaiA/AMDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267:SF1">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17645">
        <property type="entry name" value="Amdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="226" mass="25387" checksum="2443CF077F53F9CC" modified="2011-06-28" version="1">MPGGRGRIGVIIPANNAAMEYDLWKMAPEGVTIHVTRMKPTKGCEPTDVEAFRQEIKETYYLLHEVSDVIIYGRTYGTHKHGNIIKEIIKDVVIPEEAVVEVLRKLGVKKVWVGTPYIKERTLEEVSWIKSQGFEVTGFDGLGKIKGVDISNTPVFTIYRLVKRNLEDVLKADAVYIACTALSTFEASHYLHEDLGMPIVTENVAAMWKALQKIKVKGITPGFELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D0</accession>
    <name>F4B8D0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775645.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D0"/>
    <dbReference type="STRING" id="933801.Ahos_0843"/>
    <dbReference type="GeneID" id="10600311"/>
    <dbReference type="KEGG" id="aho:Ahos_0843"/>
    <dbReference type="eggNOG" id="arCOG04069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145238_0_0_2"/>
    <dbReference type="OrthoDB" id="4144at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="245" mass="28603" checksum="E64D2E8F3C3BD6BC" modified="2011-06-28" version="1">MFPFKRRKELNIAFFSSIFSIERTLTEKTLKASLLFRFFIEYRITNVYVICSSDKEYDLLREISKYALTPPYLKKYIPISPNLRKAGLLPPMNLPSHLVHRFPVEGEIRLGRNQDFGLHQKIRTTYRSIMFTDSIKGSFIQYPMIYYDGFKIHKIRFDKILEKNNLIIGSRNGKNPLKYKEEIISMYEEKGLTLLIGPPEGMLLKKLGEKFLEKSYNFIIKQGVSDVRAEEAIISSLSLLNAILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F4</accession>
    <name>F4B8F4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">secY</name>
        <name evidence="15" type="ordered locus">Ahos_0867</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775669.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F4"/>
    <dbReference type="STRING" id="933801.Ahos_0867"/>
    <dbReference type="GeneID" id="10600335"/>
    <dbReference type="KEGG" id="aho:Ahos_0867"/>
    <dbReference type="eggNOG" id="arCOG04169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031763_3_0_2"/>
    <dbReference type="OrthoDB" id="371914at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SecY/SEC61-alpha"/>
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        <property type="entry name" value="SecY_CS"/>
    </dbReference>
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        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
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    </dbReference>
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        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00303">
        <property type="entry name" value="SECYTRNLCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
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    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="97"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="134"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="14">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE93753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="465" mass="50416" checksum="46E81A9D302EAE37" modified="2011-06-28" version="1">MSFIDFLAALGKDLPAVRKPRHKPNLNQKIMWSILAVIIYLVMSSVPLYGIQSTSFSNFLIEQVIFASTAGTLAQLGIGPIITAGLIMQILVGSKLINIDLGNEDDRAKFTEAQKGLAFIFILIEASLFGYVLTKSASISSVQIELATIITAQLVAATFFILLLDEMIQKGWGLGSGVSLFIFAGVMKIIFWYMFGIVTVSSQNLPIGFFPTLISLIASHGNLLSIIVNTTKPFEPDLVGLVSTIILIILIVYLTSINIQIPVTSQRLRGIRRTIPLNFLYVSSIPVIFVSVLGADIQLFASLSSYSNSTIASILNDIVNAFEFPPPNSKIPHSVYAVVLDPVGAVIYATVFITLGVLFGLIWVEVAGLDPATQAKQLVEAGIEIPGMRSNTKMIEAILSKYIYPLAFFSSLIVSAIAVGATFLGVYGTGVGILLAVTIAIQYYSLLAYERSIEMYPLLRRLVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J5</accession>
    <name>F4B8J5_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Ahos_2076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J5"/>
    <dbReference type="STRING" id="933801.Ahos_2076"/>
    <dbReference type="GeneID" id="58788775"/>
    <dbReference type="KEGG" id="aho:Ahos_2076"/>
    <dbReference type="eggNOG" id="arCOG08322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204998_0_0_2"/>
    <dbReference type="OrthoDB" id="26561at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="58" mass="6434" checksum="7FD3B6ED22FF501A" modified="2011-06-28" version="1">MARKMCPVCKKVVEEKVVKDGDVISKVCPECGYVFISYQKGKGVLVLNNVKLSESRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L9</accession>
    <name>F4B8L9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L9"/>
    <dbReference type="STRING" id="933801.Ahos_2100"/>
    <dbReference type="GeneID" id="10601600"/>
    <dbReference type="KEGG" id="aho:Ahos_2100"/>
    <dbReference type="eggNOG" id="arCOG03847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514679_0_0_2"/>
    <dbReference type="OrthoDB" id="37074at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040581">
        <property type="entry name" value="cas_Crn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19670" checksum="A84A8FA2E24378D2" modified="2011-06-28" version="1">MVKLVATLGTSPGGVFETYMNLKSGNYGGEPVDIKEVYIIRTSDKAVELAWKLVKAIFVCCGSKEVEIADIPLPINDIATKEDYEIFRKGLQGKISKGDYVDFTGGRKAMSVAAAINAIRNSAYVVTTIIPQNEYNRIQNLIKQLKEEEIEEAGKGKCINKEKFCELVSKDARTILLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q9</accession>
    <name>F4B8Q9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fumarate hydratase</fullName>
            <ecNumber evidence="2">4.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0893</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775695.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q9"/>
    <dbReference type="STRING" id="933801.Ahos_0893"/>
    <dbReference type="GeneID" id="10600365"/>
    <dbReference type="KEGG" id="aho:Ahos_0893"/>
    <dbReference type="eggNOG" id="arCOG01749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000001">
        <property type="entry name" value="Fumarate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444:SF22">
        <property type="entry name" value="FUMARATE HYDRATASE CLASS II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarase C C-terminal" evidence="7">
        <location>
            <begin position="381"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="441" mass="48583" checksum="678DCCD545AB383F" modified="2011-06-28" version="1">MKYTEVAPKLFMNTGTRFPRKIIWAMGLVKLACARANAKLNLLDKDIAKAIEDASIEVMDGKHDEKVILDVFQTGSGTGLNMNINEIIAERASELSGKKIHPNDHVNMSQSSNDVVPTAIRIAAVSNAIENLIPSLRDILSSLNEKSKEYDNVIKPGRTHLRDALPITLGQELSAYYDALSHDLEQLNNVIEYVKELPIGGTAVGTGLNAHPKFTELVIQEINELTSLNFKTSNKFRSMRFLTDLLLLSGVMRNIAIELYRIGQDFRLMFSGPFTSIGEIDIPTQEEIAGSSIMPGKTNPVTVEATLLVSSEVVGLDHANQMASMLGEFELAMGVPLIGYNIILQENLLSEALRKFSSLVINGMKPNVEKMKRYAESSPSLITVISPIVGYDKASQIGKMLVKGMSIREALREMGFKDEEIDKILDLQQLVKPGIPAKSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R3</accession>
    <name>F4B8R3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oxidized purine nucleoside triphosphate hydrolase</fullName>
            <ecNumber evidence="11">3.6.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">2-hydroxy-dATP diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">7,8-dihydro-8-oxoguanine triphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">8-oxo-dGTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Methylated purine nucleoside triphosphate hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside diphosphate-linked moiety X motif 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">Ahos_0897</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool. Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP. Also has a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP. Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A. Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA. Through this antimutagenic activity protects cells from oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2-oxo-ATP + H2O = 2-oxo-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71395"/>
            <dbReference type="ChEBI" id="CHEBI:172878"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-oxo-dATP + H2O = 2-oxo-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:63212"/>
            <dbReference type="ChEBI" id="CHEBI:77897"/>
            <dbReference type="EC" id="3.6.1.56"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:31584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>8-oxo-dATP + H2O = 8-oxo-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71361"/>
            <dbReference type="ChEBI" id="CHEBI:172871"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:65397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>8-oxo-dGTP + H2O = 8-oxo-dGMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:63224"/>
            <dbReference type="ChEBI" id="CHEBI:77896"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:31576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6)-methyl-ATP + H2O = N(6)-methyl-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:144842"/>
            <dbReference type="ChEBI" id="CHEBI:172873"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:67609"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N(6)-methyl-dATP + H2O = N(6)-methyl-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:169976"/>
            <dbReference type="ChEBI" id="CHEBI:172872"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:67605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O(6)-methyl-dGTP + H2O = O(6)-methyl-dGMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67600"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:169974"/>
            <dbReference type="ChEBI" id="CHEBI:169975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:67601"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.56" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R3"/>
    <dbReference type="STRING" id="933801.Ahos_0897"/>
    <dbReference type="KEGG" id="aho:Ahos_0897"/>
    <dbReference type="eggNOG" id="arCOG01074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_17_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008413">
        <property type="term" value="F:8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008828">
        <property type="term" value="F:dATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042262">
        <property type="term" value="P:DNA protection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03427">
        <property type="entry name" value="NUDIX_MTH1_Nudt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003563">
        <property type="entry name" value="8ODP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43758">
        <property type="entry name" value="7,8-DIHYDRO-8-OXOGUANINE TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43758:SF2">
        <property type="entry name" value="OXIDIZED PURINE NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01403">
        <property type="entry name" value="8OXTPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="22">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00053094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="AEE93783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="134" mass="15369" checksum="1F6A3A2D1E75D9E3" modified="2011-06-28" version="1">MIKKKRGLGEGLITFPGGKVEENETPLECAIREAEEEVGIKIINPKKVGVITFKQINGNVQVMHVYLATEFIGLSKESDEAIPIWVSRNDLPFKNMWIDDRIWLPLVLEGKYVNCVFEFTDDWKFMISSLCEFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T2</accession>
    <name>F4B8T2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal-transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0916</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B920"/>
    <dbReference type="STRING" id="933801.Ahos_2172"/>
    <dbReference type="KEGG" id="aho:Ahos_2172"/>
    <dbReference type="eggNOG" id="arCOG05954">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507449_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="181" mass="21246" checksum="1131D000D0154FD3" modified="2011-06-28" version="1">MHVNSLEDLINKYGFTDEEINFALERAKGIIFGFAMEYRARKVLENYNFTNIKSVNLPTHDIEAEKDGEKYYIEVKASKKSPTKEYSAYKIAMIAQLHGIHLTLVMLPSPRLYLTEEILSEPKKVLFEFFKMLFNNENDKLKEFLANDKNRKIVESYNKVITHYFPEIKDLTSLEIIRPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B925</accession>
    <name>F4B925_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="11">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_2177</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776963.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B925"/>
    <dbReference type="STRING" id="933801.Ahos_2177"/>
    <dbReference type="GeneID" id="10601680"/>
    <dbReference type="KEGG" id="aho:Ahos_2177"/>
    <dbReference type="eggNOG" id="arCOG01358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="OrthoDB" id="372134at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="125"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="359"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE95048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="417" mass="46929" checksum="2A7BFEC0ED4E436D" modified="2011-06-28" version="1">MKVYFETYGCALNKGDTYIMMTLLKQRNHEIVNNPNEADVIVLNTCDVRLETGERMKSRIKELRKFGKKLVVAGCFSGAEPGVVKTLAPEASIIGPQALTKIIDAVEGEKIFSINAERSEITPRVFEGKIAIIPIADGCAGDCSFCITKLARRTLRSYSMRSIVEAVKEAVKNGAVEVELTGQDAAAYGLDLGGKIKLSDLLNEVLSIEGDFMIRIGMMTPEQFARDIDGILEAMRNPKLFKFLHLPVQSGDDNVLKLMNRKYTVDEYKELVKEARSKIPNINITTDIIIGHPGEDENAFENTLELMKEIKFERVHLAMYSIRPNTRSALMKQVPDSVKKERMRIAYKLYEDISLSIHKEYLNKRTKVITTEEGKKGSIIGRTLNYIPVILNGVEIGKWYDVEIIDYSFFDLRGTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B949</accession>
    <name>F4B949_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="4">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_2201</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21525"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B949"/>
    <dbReference type="STRING" id="933801.Ahos_2201"/>
    <dbReference type="KEGG" id="aho:Ahos_2201"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000001">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000010">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054948">
        <property type="entry name" value="IPMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041069">
        <property type="entry name" value="IPMS_Sufob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF9">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE95072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="314" mass="34419" checksum="7AEEA8FE5D1D4389" modified="2011-06-28" version="1">MEEVGDKTEVIGLARANKNDIDKVIEAGLSSIHVFIATSEIHMKYKLKMTREEVLDRIYESVSYAKSHGLIVEFSPEDATRSEEDFLLTAIKTAIQAGADRINIPDTVGVMTPFTFYDLIKKVVNVAGDKIVSVHCHNDFGLATANSIAGVAAGARQVHVTVNGIGERAGNASLEEVVMALKKLMHYDLNIKTWLLYETSKLVSELSGIPIPYFKAIVGENAFGHEAGIHVHGVIENPATYEPISPEEVGNFRRLALGKHSGIHGLKKILEDQGIHLDDNKLRIVLMEIKKKAESGEKISAEDARNIAIKLLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D8</accession>
    <name>F4B9D8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable oxidoreductase that may play a role as regulator of mitochondrial function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with COX5B; this interaction may contribute to localize PYROXD2 to the inner face of the inner mitochondrial membrane.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776999.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D8"/>
    <dbReference type="STRING" id="933801.Ahos_2213"/>
    <dbReference type="GeneID" id="10601718"/>
    <dbReference type="KEGG" id="aho:Ahos_2213"/>
    <dbReference type="eggNOG" id="arCOG01521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019327_0_1_2"/>
    <dbReference type="OrthoDB" id="11867at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00040298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="516" mass="56807" checksum="5E73B530709061B7" modified="2011-06-28" version="1">MYDVIIVGGGHNGLVTANYLAREGLKVAVFERREIVGGASVTEELWPGIKVSTGAYVLSLLRPKIIEDLQLKKFGLKVYTKDPGLFVPFENGKKLYIWSDIKKTQKEIEKFSKNDAKNYEKWVKFWDPLYDLADLLMLSPPVNLSNIDELLSLLKSVKIDEGTALSIARTFVTDGKSLLSEFFESDEVISALIEDAVVGTYASPSMPGTAYVLAHHVIGAVNGIKGAWGYVEGGMGGVTQALKKAAESVGVDIFESSPVDEILVDKGEVKGVKLSSGKVIESKIVVSNADPKTTFLKLLKNAEVDDELIRRIRSLKTTGVSFKIVGYSEELPDFGNGKSLSPEHIASELIMPSTEYIEKAYLDAKVLGYSREPWLSINIQSSVDPTVAPPGKFSISIFGQYLPYNKKLGEIKDKIAEITFDKIREFAHNFKLAKYEVLTPLDIERRFGIWEGNIFHLDMTPDQLYVFRPTIGLSNYSTPIKGLYLCGSGTHPGGGVTGAPGYNAAMKILSDLKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J1</accession>
    <name>F4B9J1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DadA oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777052.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J1"/>
    <dbReference type="STRING" id="933801.Ahos_2266"/>
    <dbReference type="GeneID" id="10601772"/>
    <dbReference type="KEGG" id="aho:Ahos_2266"/>
    <dbReference type="eggNOG" id="arCOG00758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_753592_0_0_2"/>
    <dbReference type="OrthoDB" id="168391at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF286">
        <property type="entry name" value="D-AMINO ACID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="360" mass="41049" checksum="11D74CF457AA8DC3" modified="2011-06-28" version="1">MKVAIVGGGIVGLFTAYFLKKEDVDITLFEKADLGSYSVHAAGLIEPYRFDKINSTSMIIKMLKYSLRGTTYIRNVNKFWLKELLKNLNKNPPEDAWKTMREMAEFSLKAYKEMAEEKNDFNYRDDGLYEVYLREEDLEKGLEEEKKSPFNPKFEKVDFEGFAGAIFFPGLSRISTEKFIDRMKNELRGVKIVNKEIQSLDELKDFDEIVLTSGIWTTKFISNLPVTAFKGYGYRVKGTPKYNKPLVLVDDGIAISPFEDFIKITGGFDADSSYDSSRAQLVLDKSSRIVDISFIYEMNMGFRPCSPDGFPIIGKRDNITVATGACRLGWSYAPAMGKYTTDLVLGRLKDLGYISRYVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K5</accession>
    <name>F4B9K5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K5"/>
    <dbReference type="STRING" id="933801.Ahos_2280"/>
    <dbReference type="KEGG" id="aho:Ahos_2280"/>
    <dbReference type="HOGENOM" id="CLU_3093901_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="51" mass="5856" checksum="819EC57FD4678511" modified="2011-06-28" version="1">MYEITNSQQVLQECSNLKYICPAFPIRVTVLIDSENYSAEVLGIKYFPLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L7</accession>
    <name>F4B9L7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxiredoxin</fullName>
            <ecNumber evidence="8">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1049</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775849.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L7"/>
    <dbReference type="STRING" id="933801.Ahos_1049"/>
    <dbReference type="GeneID" id="10600523"/>
    <dbReference type="KEGG" id="aho:Ahos_1049"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_2"/>
    <dbReference type="OrthoDB" id="6924at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009668">
        <property type="entry name" value="PRK13189.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009670">
        <property type="entry name" value="PRK13191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF121">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="8"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="8">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="9">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="227" mass="25998" checksum="55605BC5561D9583" modified="2011-06-28" version="1">MSEAYRIPLIGEKFPEMEVDTTHGKIKLPEAYKGKWFVLFSHPGDFTPVCTTEFVSFAKKYEEFKKLNTELIGLSVDSNISHIEWVTWIEQNLKVEIPFPIIADPMGNVAKRLGMIHAESSTAAVRAVFIVDDKGTVRLIMYYPLEIGRNINEILRSIKALQIVDRTGAVVPANWPKNEIIGDNLLNPPPRTVKDSKLRLQQFKGYAWWLTYKEADKKDVEEAQKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q9</accession>
    <name>F4B9Q9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">B3/4 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775891.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q9"/>
    <dbReference type="STRING" id="933801.Ahos_1091"/>
    <dbReference type="GeneID" id="10600565"/>
    <dbReference type="KEGG" id="aho:Ahos_1091"/>
    <dbReference type="eggNOG" id="arCOG04250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076869_2_0_2"/>
    <dbReference type="OrthoDB" id="35982at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39209">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39209:SF2">
        <property type="entry name" value="CYTOPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B3/B4 tRNA-binding" evidence="1">
        <location>
            <begin position="58"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="220" mass="24416" checksum="5AAAC9610520C694" modified="2011-06-28" version="1">MNIVVSEDCKNLGIFIAHTEVLGVNNSKGKLEEEMRKVEEMYSQEDPEKLKINPIVRAYRDFYWKIGIDPTKVRPAGEALRRRINRSGKLPRINDIVDAGNIASSSTLVPIGIYDISKIIGEPSIILSKGGEEFYAIGKDKPEILQKGIPIMVDKEKVMHIYPHRDSKITSVTDITRDILIVGAGVPGVPQELVGNAVNIVVELVLKYLGGKQTHQVVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R7</accession>
    <name>F4B9R7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxypeptidase regulatory-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775899.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R7"/>
    <dbReference type="STRING" id="933801.Ahos_1099"/>
    <dbReference type="GeneID" id="10600573"/>
    <dbReference type="KEGG" id="aho:Ahos_1099"/>
    <dbReference type="eggNOG" id="arCOG05997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976577_0_0_2"/>
    <dbReference type="OrthoDB" id="34256at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49478">
        <property type="entry name" value="Cna protein B-type domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14097" checksum="F7C21CA49102EE29" modified="2011-06-28" version="1">MNKNLLLALGIIILLLTAVGVYMASSYKTTISITNLPSSSSNEETLEVKVLMNYGPLGGVSPLSDAYVEIYNSQGYYVTANFTNSQGVAIFHLPPGNYKVFVTQLHCSVNVSLTSSKEVTISYAYLYAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T6</accession>
    <name>F4B9T6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ahos_1118</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775918.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T6"/>
    <dbReference type="STRING" id="933801.Ahos_1118"/>
    <dbReference type="GeneID" id="10600592"/>
    <dbReference type="KEGG" id="aho:Ahos_1118"/>
    <dbReference type="eggNOG" id="arCOG01136">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065946_3_1_2"/>
    <dbReference type="OrthoDB" id="30609at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000042">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="7"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE94002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="112" mass="12244" checksum="2C6B103EF875A0D0" modified="2011-06-28" version="1">MEISIDDFSKVELKVGLIKSAERIEGTRLLKLIVDLGSEERQIISGIAEYYSPEQLIGKKVIVVANLKPKMIRGFESQGMILAAGCKEDEDKGIKPTLLTVDGDVPPGTRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U4</accession>
    <name>F4B9U4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferritin-like diiron domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777075.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U4"/>
    <dbReference type="STRING" id="933801.Ahos_2289"/>
    <dbReference type="GeneID" id="10601797"/>
    <dbReference type="KEGG" id="aho:Ahos_2289"/>
    <dbReference type="eggNOG" id="arCOG01098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100475_0_0_2"/>
    <dbReference type="OrthoDB" id="43037at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="182" mass="20537" checksum="A1F5CCEC7A468E4E" modified="2011-06-28" version="1">MALGKETEMGLKELFKANAEDYLTLTFLADKLEALGRKEEARLLREKARVEYGHAMGIFEKLLQNADVTKLLGEFVKEEDQEHVAEYDNVAMKAKEEGHLDVEKMLCSYAEQEKDIAESTAKVLAMLSDFAREEDMEHVAEYNDVAMKAKEEGHADIEAMLCAYAEQEKQIAETAKKVAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA28</accession>
    <name>F4BA28_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
            <shortName evidence="7">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">serS</name>
        <name evidence="12" type="ordered locus">Ahos_1141</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>tRNA(Sec) + L-serine + ATP = L-seryl-tRNA(Sec) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>tRNA(Ser) + L-serine + ATP = L-seryl-tRNA(Ser) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775941.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA28"/>
    <dbReference type="STRING" id="933801.Ahos_1141"/>
    <dbReference type="GeneID" id="10600615"/>
    <dbReference type="KEGG" id="aho:Ahos_1141"/>
    <dbReference type="eggNOG" id="arCOG00403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_2"/>
    <dbReference type="OrthoDB" id="35932at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000048">
        <property type="entry name" value="Serine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="205"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="69"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="252"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="283"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="299"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="370"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="455" mass="52833" checksum="6B8D9A5393E91B82" modified="2011-06-28" version="1">MSWSLLELIRNNPDELIDSLKKRNMDTSLALKAVELDKKWRSILQEVEKLRHQHNEINSQIPKLKPEERKAKIEEAKKLLQILEAKEKELKEIEEQRDEILRSLPNIISPDVPVGPDDTYNVPIKFWGKFKVYEKDLEEFKKQLNGAQVEYEIINWKLVGHADMLENVLKLGNTEKASQVAGSRFYYLFDDLVWLDFALLLYAIDTMTSKGYRLVLPPYMLRGEVIHAVIDLDTFKDAIYKIENDDLYLIATAEHPLAALYYKEDIEKEELPIKLVGISPAFRREAGAANKDLKGIFRVHQFHKVEQFIFSLPEDSWKYHQELLSNAEEIFQGLGIPYRIVNIASGDLGACAAKKYDLEAWMPAQAKFREMVSCSNCTDWQAFRMKIRYVDRKNNKKGYVHTLNSTAVASTRTITAILENFQNEDGTVEIPKVLRKYLEPFKAAPKDYIYPRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA31</accession>
    <name>F4BA31_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="10">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="10">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">ImGP synthase subunit HisH</fullName>
            <shortName evidence="10">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hisH</name>
        <name evidence="13" type="ordered locus">Ahos_1144</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="10"/>
    <dbReference type="EC" id="3.5.1.2" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775944.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA31"/>
    <dbReference type="STRING" id="933801.Ahos_1144"/>
    <dbReference type="GeneID" id="10600618"/>
    <dbReference type="KEGG" id="aho:Ahos_1144"/>
    <dbReference type="eggNOG" id="arCOG00089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_2_2"/>
    <dbReference type="OrthoDB" id="33401at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701:SF1">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 10" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="12">
        <location>
            <begin position="8"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000495-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="195" mass="21625" checksum="EF89B8A9445269D5" modified="2011-06-28" version="1">MKALVVNYGVGNLFSISSGLKRAGFEVEISKEIKNADLIVFPGVGAFSAVAKFIQINSDKINDLRKSGVHFLGVCLGLQIMFEKGTEGGESKGLGWFKGTVDKIHANVKLPHIGWDKIYVSGDCELTEGLDGKYAYYVHSYVAYTNDHVVMKSNYGIEYPAMVCKENVVGTQFHPEKSSETGRIFFDNLKRWVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA52</accession>
    <name>F4BA52_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="12">KARI</shortName>
            <ecNumber evidence="12">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="12">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ilvC</name>
        <name evidence="16" type="ordered locus">Ahos_1166</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH or NADH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NAD(+) = (2S)-2-acetolactate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.383"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.383"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 13">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA52"/>
    <dbReference type="STRING" id="933801.Ahos_1166"/>
    <dbReference type="KEGG" id="aho:Ahos_1166"/>
    <dbReference type="eggNOG" id="arCOG04465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000023">
        <property type="entry name" value="Ketol-acid reductoisomerase (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004017">
        <property type="entry name" value="PRK05479.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 12 13" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6 12 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="7 12 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="14">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="15">
        <location>
            <begin position="183"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE94049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="332" mass="36975" checksum="C1F6A8CB6FD9413F" modified="2011-06-28" version="1">MAKIYTDQDASLDPIKDKKIAVLGYGNQGRAWALNLRDSGLNVIVGLEREGNSWKQAVQDGFQPVHTEDAVKQADIIIFLIPDMVQRYVYREKVQPNMKEGADLVFAHGFNIHYKLIDPPSTSDVYMVAPKGPGATVRDFYTKGGGVPALVAVQQNPSGKALEKALAIAKGIGATRAGVIETTFKEETETDLFGEQTTLVGGVMELMRSAFRTLVELGYQPEVAYFETINEMKMIVDIIYEKGFTGMLKAVSDTAKYGGLTVGKFVINEEVRKRMLEAAEKIRSGKFAEEWIEEYNRGSPTIKEGLEEIENSLEEQTRRRLKEIIERGKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA55</accession>
    <name>F4BA55_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="7">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxT</name>
        <name evidence="10" type="ordered locus">Ahos_1169</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EC" id="3.5.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775968.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA55"/>
    <dbReference type="STRING" id="933801.Ahos_1169"/>
    <dbReference type="MEROPS" id="C26.A32"/>
    <dbReference type="GeneID" id="10600643"/>
    <dbReference type="KEGG" id="aho:Ahos_1169"/>
    <dbReference type="eggNOG" id="arCOG00034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_2"/>
    <dbReference type="OrthoDB" id="26717at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000041">
        <property type="entry name" value="Glutamine amidotransferase subunit pdxT, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7 8">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="144"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="199" mass="21710" checksum="34B98660494D70F9" modified="2011-06-28" version="1">MKIGVLAYQGSFEEHALQAKRAMDKLKIDGEIIAVKRVNDLDVDGIIIPGGESTTIGIVAQRMGLLEPLKEKIMEGLPVLGTCAGAIMLAKDVSDAKVGKKSQPLIGVMDISVIRNYYGRQRESFEANVDLRSIGGGIEKVVFIRAPAIVKVFGNAKSLSQFKDVHVMVQENNLLATTFHPELSGTTIVHEYFISMIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA74</accession>
    <name>F4BA74_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775987.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA74"/>
    <dbReference type="STRING" id="933801.Ahos_1188"/>
    <dbReference type="GeneID" id="10600664"/>
    <dbReference type="KEGG" id="aho:Ahos_1188"/>
    <dbReference type="eggNOG" id="arCOG04213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050011_0_0_2"/>
    <dbReference type="OrthoDB" id="80661at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052199">
        <property type="entry name" value="MIPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125:SF1">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="1">
        <location>
            <begin position="180"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="344" mass="38080" checksum="5BB5AA36240D6508" modified="2011-06-28" version="1">MIRVGLVGIGNVASALVQSIEMVKQGIEIPGILNLPIKADEIEIVTAFDIDKRKVGKKLSEAIFSKPNVVQKYVDVKSDVEVLRGPTLDGVDEQLSKVIEESEEKPVNVKEVLKENNVDVVINLLPAGADKASLFYAEESLSANSSFINATPSNVTERVGDKYKEAKIPIFGDDLLSQIGGTIFHSGIIEFLQKRGVKILRSYQIDIAGNTETFATLEDWKKDLKKKIKSSFISQRSDNAEIVAGTSDYVEFLGDRRVSYMVIEGEYAMGAKVRVDISLKTYDAPNAVQPLIDLIRLAKILKDNNIGGVIPEVCGYYFKNSPVRYKSIDEAKARFDDFLKKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R2</accession>
    <name>F4B3R2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776015.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R2"/>
    <dbReference type="STRING" id="933801.Ahos_1216"/>
    <dbReference type="GeneID" id="10600692"/>
    <dbReference type="KEGG" id="aho:Ahos_1216"/>
    <dbReference type="eggNOG" id="arCOG01049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062599_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052899">
        <property type="entry name" value="Class-I_DAHP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006268">
        <property type="entry name" value="DAHP_syn_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01361">
        <property type="entry name" value="DAHP_synth_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006421">
        <property type="entry name" value="PRK08673.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009239">
        <property type="entry name" value="PRK12595.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43018">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43018:SF2">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="2">
        <location>
            <begin position="75"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="329" mass="36134" checksum="0902CD79C23B9340" modified="2011-06-28" version="1">MLFILRNDSSTLMEKIKESSASYKFLDLYGKKLALVWPDEEAEKINDDSIEMKVKTRKPYILASNEWKKEPTKVNVKDVEIGGEKIVVAAGPCAVESEEQVETVAKAVKRAGASLLRGGAFKPRTSPYSFQGLGEGGLKILRKVSDEVGLPIVTEIMDARDLPLFKQYDIDMLQIGARNAQNFTLLKEVGKFGKPVLLKRGMANTVEEWLQSAEYLLSEGNGNVVLCERGIRTFEKATRFTLDIGGMVAAKMMTHLPMCADPSHPAGKRELVHSLALASVAAGADMLIIEVHPNPEKALSDSEQQLTPESFEVLMNRIKALANALGRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R6</accession>
    <name>F4B3R6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBA/THIF-type NAD/FAD binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776019.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R6"/>
    <dbReference type="STRING" id="933801.Ahos_1220"/>
    <dbReference type="GeneID" id="10600696"/>
    <dbReference type="KEGG" id="aho:Ahos_1220"/>
    <dbReference type="eggNOG" id="arCOG01676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_1_2"/>
    <dbReference type="OrthoDB" id="7915at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="289" mass="32523" checksum="1210C8A852CEF60B" modified="2011-06-28" version="1">MDRFSRQLLTLGIDVQEKIMSTKVLVAGCGALGSSIAELLVRLGVKELKIVDADVVELSNLHRTHLFTEKDLMKPKVLACKEFLEKINSEVKIEPIFDIIDETNAEDLVKDVDVVFDGLDNINYRLILNDACVKYEKPLIHAGVSGEYGNAKLVIPGKTSCLACFLQPSDSRNACDIIGTTTVVPNFLASIQVQLFINYLRGYSEDELVIADLKDLRLDKIKMKRNPQCEACSLHEYKYLRHMDINCGLTRAEKAEGDRIFSSNGVELYKYNGGYIICYNQKCFKKRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U3</accession>
    <name>F4B3U3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="4">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pcn</name>
        <name evidence="9" type="ordered locus">Ahos_1247</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776046.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U3"/>
    <dbReference type="STRING" id="933801.Ahos_1247"/>
    <dbReference type="GeneID" id="10600726"/>
    <dbReference type="KEGG" id="aho:Ahos_1247"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043978_1_1_2"/>
    <dbReference type="OrthoDB" id="14749at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002220">
        <property type="entry name" value="PRK01115.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="8">
        <location>
            <begin position="130"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="244" mass="26986" checksum="B8307A23DA3376FC" modified="2011-06-28" version="1">MKFKALDAPAFVYIIKTISEFMDEGAIVSTNDGIRINGIDPSRVVFLDIFLPAGYFENYESKDKEVIGINLEDLSSILARVKKDDALSFETDGSNLKIIIEGSFQRIFSLPLLSMEEQKNPSLNLEFPFKAKMLTVTFSDVMDGLADLGDTLVVSSEGGKLYLSVEGDMGESKVELSTDNGALLEASGNDAKSTYGMEYVLNTTKMRKASDTMELYFGSQIPLKLHYELPQGGYGEFYIAPRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V4</accession>
    <name>F4B3V4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1258</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776057.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V4"/>
    <dbReference type="STRING" id="933801.Ahos_1258"/>
    <dbReference type="GeneID" id="10600739"/>
    <dbReference type="KEGG" id="aho:Ahos_1258"/>
    <dbReference type="eggNOG" id="arCOG01263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_2"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="244" mass="26976" checksum="5C48E1F48A432720" modified="2011-06-28" version="1">MIAVVTGASKGIGKATVIKLKDLGYTVVAISRSLPEIGDEKIQADVSNKEDVFKTVNYLISKYGFIDVLVNNAGFGVYGSFLETDLNEEEYMIRTNLLGPIYFMKAVLPYMVKERRGSIVNIISEAAYVSSPKLLVYSATKAALAHITNGLWAEMRKYGVKVSGIYPGPVKTNFTSHPSFKGAKDSFSRFAVEPEKVANAVVKAIKTGKREIYVPSKLALDPYFLKLSYIMQSITYRFAGKYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B429</accession>
    <name>F4B429_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774910.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B429"/>
    <dbReference type="STRING" id="933801.Ahos_0100"/>
    <dbReference type="GeneID" id="10599540"/>
    <dbReference type="KEGG" id="aho:Ahos_0100"/>
    <dbReference type="eggNOG" id="arCOG03462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034270_0_0_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF7">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE92993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="529" mass="58888" checksum="D819150E70313E30" modified="2011-06-28" version="1">MAQRKDLFIRESSGLVREVSPWASLSATFGLVTGGVPILILSWLFTAPGANWVLSFLLMLPPTLGMAFLFYIAGISMPRAGGDYVFNSRAVHPAIGFINYFGLFVAFALSLGYYSYLAAQWFGYLFSSIGLAYSNQFFLSLGCWFSSTTGSVVIGILVVFLSIILSIIPRVQWRFVFWGGVITLVSSIIMFIFLLQITPTSFSTALSSSTGIPNAYNEVINDAMSNGLKFYPSLYATFLAGPVIWYYYTWYNLPASWSGEMKQVRKNVLYSIIVAILIISAYYILFTYINLRAFGCRFLTSWSYISNCGINDPVYKSLSSIGDFTPYFALIVDHNLLIFMIMFIALWLPNFYSNPPLVTSLVRYLFSWSFDRLMPSWLADVNENLKAPVKATLLVGAMGIIGVLLYAFNTVVALVDVTVIFEIGYAIFALSVALMPYVRKQVYERSVPVKRKILGIPLVSLIGVPVFAFLMFILAITWGNPILLPINIQTLASLGIIYAVGGIIYAIATQLNKKKGIEVSLLFQEIPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B435</accession>
    <name>F4B435_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774916.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B435"/>
    <dbReference type="STRING" id="933801.Ahos_0106"/>
    <dbReference type="GeneID" id="10599546"/>
    <dbReference type="KEGG" id="aho:Ahos_0106"/>
    <dbReference type="eggNOG" id="arCOG01469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096331_0_0_2"/>
    <dbReference type="OrthoDB" id="97972at2157"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC-TYPE MULTIDRUG TRANSPORT SYSTEM, PERMEASE COMPONENT"/>
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    </dbReference>
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        <property type="entry name" value="NODULATION PROTEIN J"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="240" mass="26752" checksum="8A7B3E5B14088D40" modified="2011-06-28" version="1">MTRSNFILAFAWFYGYSNIRRAPIYILAYLSLPLSLLFFIYIISKGELLAYGIVGGLISVIVSNSISLIGDFAFLRLQLRLQDLLVATEIGPMDYILGLTLGNFIFTLPGVLVYIILGLIFHVFSAFQLLAILGISAILLISTTSLSITLASLIKHTRHSWGLSTFLSLIFTILPPLYYPYSILPKDVLYALLLSPSTSASMIVQGLLNLQKMSLLAIVVFILESVVFISLPRYAMKWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B462</accession>
    <name>F4B462_ACIHW</name>
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        </recommendedName>
    </protein>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_1287"/>
    <dbReference type="GeneID" id="10600768"/>
    <dbReference type="KEGG" id="aho:Ahos_1287"/>
    <dbReference type="eggNOG" id="arCOG04177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:1990904">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="51" mass="5839" checksum="BE2A7A6C08811837" modified="2011-06-28" version="1">MSTTKPLAKKLRLAKALKSNSAIPAWVILKTNGKVRINPLRRNWRRNSLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B463</accession>
    <name>F4B463_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL31</fullName>
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    </protein>
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        <name evidence="5" type="primary">rpl31e</name>
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        <lineage>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
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    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10600769"/>
    <dbReference type="KEGG" id="aho:Ahos_1288"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="36822at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal_eL31"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="88" mass="10126" checksum="9E04BE8C2BCE6841" modified="2011-06-28" version="1">MKEKDNFEMVINLRKIATGKRTNRLGRALKEIRSKIIRHFGADRVVIDPLVAKSLSSNGYDKIRSRIRVVVSKLDEKTYLVKLAIKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B489</accession>
    <name>F4B489_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clan AA aspartic protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1314</name>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776113.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B489"/>
    <dbReference type="GeneID" id="10600798"/>
    <dbReference type="KEGG" id="aho:Ahos_1314"/>
    <dbReference type="eggNOG" id="arCOG04029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176719_0_0_2"/>
    <dbReference type="OrthoDB" id="43977at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11499" checksum="32A1C1E28BA27AB9" modified="2011-06-28" version="1">MRVKLTVENVEELFLIDTGFSGDLIVKSDVFDEISAKPSDGGEICVAKDLCFSALQKVVSVNILSKQILCRALWVPEIEENIIGEGVLIKAGLLLDYKNFAISDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B498</accession>
    <name>F4B498_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776121.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B498"/>
    <dbReference type="STRING" id="933801.Ahos_1323"/>
    <dbReference type="GeneID" id="10600809"/>
    <dbReference type="KEGG" id="aho:Ahos_1323"/>
    <dbReference type="eggNOG" id="arCOG01840">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151087_0_0_2"/>
    <dbReference type="OrthoDB" id="37046at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13862" checksum="D7931B813C3A4814" modified="2011-06-28" version="1">MPAFRDLYDLVKKMMEEEEKEFKKMEEEIKKEFNEPLYTYYETDKEIYYIVDVPHLESQTLYIKMENNKLELSCKDKYGKCYHLELPVNNVEKYDVDVVRNKGFIKIILKKKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B4</accession>
    <name>F4B4B4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1339</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776137.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4B4"/>
    <dbReference type="STRING" id="933801.Ahos_1339"/>
    <dbReference type="GeneID" id="10600825"/>
    <dbReference type="KEGG" id="aho:Ahos_1339"/>
    <dbReference type="eggNOG" id="arCOG01754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_2"/>
    <dbReference type="OrthoDB" id="7437at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05303">
        <property type="entry name" value="PGDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="6">
        <location>
            <begin position="18"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="120"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="324" mass="35903" checksum="32070660C6F2E625" modified="2011-06-28" version="1">MEQTTVQTNSLNKENLNILITDPVADVMLNTLKEKGFHVDYQPEISKEELMKVIENYDIIVVRSRTRVTRDIIERGKRLKIIARAGIGLDNIDTDAAQERGIKIVYAPGASTDSAVELTIGLMITGARKMYTSMTLAKSGIFKKIEGLELAGKTLGIIGFGRIGTKVGIIAKAMGMRVLAYDIMDIKDRATQLGIIPVTLEELLKQSDVISLHVTVGKNAKPILDEKAFEIMKDNVIIVNTSRAVAVDGQALLKYIKSGKVYAYAADVLWHEPPKEEWELELLKHERVTITTHIGAQTKEAQYRVAVTTTENLLNAIKELGVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B9</accession>
    <name>F4B4B9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776142.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4B9"/>
    <dbReference type="STRING" id="933801.Ahos_1344"/>
    <dbReference type="GeneID" id="10600830"/>
    <dbReference type="KEGG" id="aho:Ahos_1344"/>
    <dbReference type="eggNOG" id="arCOG04062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152335_0_0_2"/>
    <dbReference type="OrthoDB" id="33530at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="126" mass="14583" checksum="FE06DA201897A79E" modified="2011-06-28" version="1">MVQLYVENSKSKSGKHAIRTLLYKVVDGKLIEVKDEGNKVSPTYKVGEAKVINISDNGIYIYVKLVKNIYNRIIGEILVIDNNSIVLKLKYRKLKIKKIEGDEKYFDKVKELFEKLKIPIKRANLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K3</accession>
    <name>F4B4K3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1350</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776148.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K3"/>
    <dbReference type="STRING" id="933801.Ahos_1350"/>
    <dbReference type="GeneID" id="10600836"/>
    <dbReference type="KEGG" id="aho:Ahos_1350"/>
    <dbReference type="eggNOG" id="arCOG02014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_9_2_2"/>
    <dbReference type="OrthoDB" id="25514at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010089">
        <property type="entry name" value="PRK13574.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="6">
        <location>
            <begin position="156"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="418" mass="47333" checksum="620BE39E64E93473" modified="2011-06-28" version="1">MNAYPITSFAQPYEVFKCVRANEDFAALLESVSGPQNKARYSIIAWGKKDYLSSEYSEDIADKLYSISTYGGFSIPEFNAYIGYVSYDAVRNWETVKDIKPVAESWPNGEFFLPENVIIYDHNSAKVYVNGDISGISSCGEIGDIKFSGYDQSMNKNEFEDAVNEILEYIRNGYAFQVVLSRFYRFTFKGDLMRFYYNLRKINPSPYMFYLKFKERELIGSSPELLFSVQNGVVESYPIAGTRPRGSTPEEDFELEQELLSSEKERAEHLMLVDLARNDIGRVCYPGTVKVPDFMYIEKYSYVQHIVSRVIGTLKKGFTPVDVLKSTFPAGTVSGAPKPMAMNIIETLEPFKRGPYAGAVGFISKDSAEFAISIRSAFVNKDLFRIQAGAGIVYDSIPEMEYFETEHKMRALKVAMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M7</accession>
    <name>F4B4M7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thermosome</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1374</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776172.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M7"/>
    <dbReference type="STRING" id="933801.Ahos_1374"/>
    <dbReference type="GeneID" id="10600861"/>
    <dbReference type="KEGG" id="aho:Ahos_1374"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="539"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="559" mass="60249" checksum="F795608E068D9BC4" modified="2011-06-28" version="1">MASAGGVPVLLFKEGTSRSSGREALKNNILAARTLAEMLKTSLGPKGLDKMLIDSFGDVTITNDGATIVKEMEIQHPAAKLLVEAAKAQDAEVGDGTTSAVVLAGLLLEKADALLDQNIHPTIIIEGYKKAFTKSLELLSQIATKLDVSDLNSSTTRENLRKIVYTTMSSKFLAEGEEFNKIMDMVIDAVATVAEPLPTGGYNVSLDLIKIDKKKGGSIEDSQLIKGIVLDKEVVHPGMPRRVEKAKIAVLDASLEVEKPEISAKISITSPEQIKAFLDEEAKFLKDMVDKLASIGANVVICQKGIDDIAQHFLAKKGIMAVRRVKRSDIEKLEKALGARIISNIKDASPEDLGYAELVEERKVGNDKMVFIEGAKNPRAVNILLRGSNDMALDEAERSINDALHALRNILLEPMIVPGGGAIELELAMRLREYARTVGGKEQLAIEAYAEALEEIPMILAETAGMEPISTLMELRAKHAKGLVNAGVDAINGKIVDDIYTLNVVEPIRVKRQVLKSATEAATAVLKIDDLIAASQLKSSKEGEKGGEKKEGEESSLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P5</accession>
    <name>F4B4P5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776190.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P5"/>
    <dbReference type="STRING" id="933801.Ahos_1392"/>
    <dbReference type="GeneID" id="10600881"/>
    <dbReference type="KEGG" id="aho:Ahos_1392"/>
    <dbReference type="eggNOG" id="arCOG04153">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142666_0_0_2"/>
    <dbReference type="OrthoDB" id="31046at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13808" checksum="83239C4C96FC5380" modified="2011-06-28" version="1">MESNISSYENLIQEKILEKGNEGISQQELAKSVGLSTRELSMIVKRLIEKKVIVKRTVKENGKSVVKLFAAKSSSINNDIYINLDTVKEIPCFSCKLLYKCNNGAHVNPGSCGKLSEWILYLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S7</accession>
    <name>F4B4S7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxiredoxin (PRX) family, AhpE-like subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
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                <person name="Prangishvili D."/>
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                <person name="Prangishvili D."/>
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    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000503">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000021">
        <property type="entry name" value="Drug resistance transporter, EmrB/QacA subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="6"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="469" mass="49865" checksum="2AFB1ADB30FBBE5E" modified="2011-06-28" version="1">MNQKIVLLVLSLGTLMAAVDTTIVLLALPTITDCLHTNLFSSIWVLLAYLIVLAVLSTQAGRIGDLIGRGKIYNFGFILFTLASALAGISPNVCLLISFRVIQAIGGALLTSNSYAIIADVFPPNSRGKAYGITALGWNVGALLGIVLGGVLTTFFGWRFIFYINVPIGIISVILGIREIRDSRVVKNKLDITGSVILGVSLALISVGSMMIAGIGVDIMDVVEIILGVALLPIFVINESKVQSPIINLKLFKIRLLSFSLLASLFQGIGALSITFLLIMYLQGVRGLTPLQSSLLLTPGYVVASVLAPFMGRIADRGRPGLLAGIGLLFIFASLMLYYFILTPSTPYFTILGITAITGIGSAMFWPSNATAVMFSAPKEYYGSVSGVSRTLGSIGTTLSYVMSITVATLVIPRQVAFEIFLGTNVLDGDVSNAFVNGLHFAFLISAIIIIIATLFSVLGGSTNVKRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B542</accession>
    <name>F4B542_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Threonyl-tRNA synthetase editing domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1461</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776259.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B542"/>
    <dbReference type="STRING" id="933801.Ahos_1461"/>
    <dbReference type="GeneID" id="10600957"/>
    <dbReference type="KEGG" id="aho:Ahos_1461"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712936_0_0_2"/>
    <dbReference type="OrthoDB" id="372136at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011500">
        <property type="entry name" value="PRK14938.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Threonyl-tRNA synthetase editing" evidence="4">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon-binding" evidence="3">
        <location>
            <begin position="281"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="385" mass="44252" checksum="EB30433B21BC7E32" modified="2011-06-28" version="1">MIQLFIHASNFSYEVKEKAVEKAEEDYLPSLKKENALVVFTTVEKGDDEEIIRKAVENIKDIFSKVKASCVIIYPYAHLSNNLSSPDVAISSLKEIEKELKDSGIETYRAPFGWYKAFSISCYGHPLSELSRRITKSEEFSKSEELEICNKFGFPSSPKATFMKIATLEYLKKELSPSSIIISNDELKEKEGTMIIRYLKPSGRILPCINEDPKIEVIYFGEKQLDFPKEFKDSKNSLKIWESKDGKTTIWVGNLIYYILLQAKSMSTTPYLPLWISPIHVRLLPVKKDFLEKTEEFANQLLSKGVRVEIDNKDDGLGNKIRRSGMDWIPYVAIVGEREIKTSTLTVRIRKKDEQKSLTVDELYALIKDEDPLMLRQNTPVKMFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B554</accession>
    <name>F4B554_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1473</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776271.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B554"/>
    <dbReference type="STRING" id="933801.Ahos_1473"/>
    <dbReference type="GeneID" id="10600972"/>
    <dbReference type="KEGG" id="aho:Ahos_1473"/>
    <dbReference type="eggNOG" id="arCOG01408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616268_0_0_2"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="238"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="433" mass="48720" checksum="2BB0418798C3CE72" modified="2011-06-28" version="1">MRITINTQTPPIRFRLSYKDIVEKYGELDLPLDISMLSSEDYYISVGGVARMMLGLIDKFGGKAKWVALGPGYPPYVRMNNIDVYFVDLDPKSLAGYTRFKEGIYNESHGIARYNLNGRDYIDYATYNWLSAQKLLELYSDTDVYFINDFQQLLVGGIIGPSAPAVLWYHIPFVPEMLNEKLRNFLIRSFEGFDAVIVSTKRDLEGLVRTGAKVKVKQIYPYIDPSYYNTNVSKSDVENVERKFGINDDDKVVLVVARMDPIKSQDVAIQAIKNLDVKLVLAGDGSFTSKSLGHDKASIWSRKLRELARDLKVENKVIFTGYVSDEELMALYKRANAVALPSRIEGFGLTVCEGWLFKKPVVVSKGAGVSELVIDGGNGYTFRTYEEMAEGISKALKEEDKLGSLGYETVKKCLIDNEFDSIKAVLEEAASEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B594</accession>
    <name>F4B594_ACIHW</name>
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            <fullName evidence="1">Circularly permuted ATP-grasp type 2 domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="2" type="ordered locus">Ahos_0297</name>
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    <organism evidence="2 3">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B594"/>
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    <dbReference type="GeneID" id="10599737"/>
    <dbReference type="KEGG" id="aho:Ahos_0297"/>
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    <dbReference type="InterPro" id="IPR016450">
        <property type="entry name" value="UCP005522"/>
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    <dbReference type="PANTHER" id="PTHR34595">
        <property type="entry name" value="BLR5612 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34595:SF7">
        <property type="entry name" value="SLL1039 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14403">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Circularly permuted ATP-grasp type 2" evidence="1">
        <location>
            <begin position="66"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="436" mass="49838" checksum="50C024A10BD12979" modified="2011-06-28" version="1">MIKIKRTNKASFLEYEEEHYKTLLSDLERIPDFFKYVQLINELAYREGFTFYTGNYYRSIKIDPIPRILKKEEFWKISEGLKSRAEAINKFLYSYYHGDKVVVPEDLIETSPYFRPEMMGFDPPKGIYVYIFGEDLVKVKGIPYILEDNVRIPSGMSYAIKAHELTERVLGDKFSVKGNEGDGLEKLKKTLTFASDTRDPVIAILTDGTLNSAYFEHKFYSDKLDLLLVEPSDINIREGEVTVSTLEGEVHVDVIYRRIEDLDFLTPGLMNAYLRGWVNIVNAPGTGIADDKITFCFMPQIMEELGIKEGVKQPFSFPIGTTREDIEEKIKNMVVKKREGYGGSGTFVLHDMSEEEKKKVLNEVRKSPEEFMAQELLDFDTVISAIGDSFYQAYADLRFFVFVNESSTSILSRVAPVGSRVTNNSSGGLVKPLWIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B596</accession>
    <name>F4B596_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7347 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775106.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B596"/>
    <dbReference type="GeneID" id="71811272"/>
    <dbReference type="KEGG" id="aho:Ahos_0299"/>
    <dbReference type="eggNOG" id="arCOG03859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392712_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055771">
        <property type="entry name" value="DUF7347"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24038">
        <property type="entry name" value="DUF7347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7347" evidence="1">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="11018" checksum="E3D32EE090DD59FF" modified="2011-06-28" version="1">MYENVMKSLVKENGFKEYSRDLLMDLNRIIKDKTRKNILIYLKNKGKAAYSEILRDMKLSTGKLNYHLKILELLIEKEGEYYKLNEKGNNSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B2</accession>
    <name>F4B5B2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Archaeal PaREP1/PaREP8 family</fullName>
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    <gene>
        <name evidence="1" type="ordered locus">Ahos_0315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775122.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B2"/>
    <dbReference type="GeneID" id="10599756"/>
    <dbReference type="KEGG" id="aho:Ahos_0315"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="OrthoDB" id="37218at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="175" mass="20277" checksum="A3FBBF9742AF10F7" modified="2011-06-28" version="1">MEKDLYKVGEDYVEARIIESLSDLLLSLTLWKEGYTRNSAGKAFSAVKALLSALIITNEEKLINLAKDDKEKKWIKKKAHIVPTHAMYGLAQRLKDIGIDVINIVNYALNLHDYQYNGFEPGFSRYSKKEEVFRDLITLVNETRKVIDAYYSKYEVKEILEKIDELIKELIEGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D1</accession>
    <name>F4B5D1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D1"/>
    <dbReference type="STRING" id="933801.Ahos_0334"/>
    <dbReference type="KEGG" id="aho:Ahos_0334"/>
    <dbReference type="eggNOG" id="arCOG08402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207589_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="43" mass="4512" checksum="BF710C6F7ED64429" modified="2011-06-28" version="1">MTGTAHEGGSDKSVDGRVVVFPSTSPKDLRVNVYDPLRGVPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M7</accession>
    <name>F4B5M7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M7"/>
    <dbReference type="STRING" id="933801.Ahos_1568"/>
    <dbReference type="KEGG" id="aho:Ahos_1568"/>
    <dbReference type="eggNOG" id="arCOG01985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163428_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012206">
        <property type="entry name" value="Fd_FixX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082">
        <property type="entry name" value="FERREDOXIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43082:SF3">
        <property type="entry name" value="FERREDOXIN-LIKE PROTEIN YDIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036548">
        <property type="entry name" value="Fdx_FixX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10356" checksum="B2CA9BA6C399075A" modified="2011-06-28" version="1">MESSKIVPLLKRLGLNTYNVDKKSHIEVNTDICINCKDKPCTVSCPAGTYEALPDGRIAVHYERCLECGGALVICPFGAIKFRFPEGGISYRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M8</accession>
    <name>F4B5M8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1569</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776367.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M8"/>
    <dbReference type="STRING" id="933801.Ahos_1569"/>
    <dbReference type="GeneID" id="10601070"/>
    <dbReference type="KEGG" id="aho:Ahos_1569"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050977_0_0_2"/>
    <dbReference type="OrthoDB" id="7950at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039651">
        <property type="entry name" value="FixC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43624:SF2">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-QUINONE OXIDOREDUCTASE YDIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12831">
        <property type="entry name" value="FAD_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="407" mass="45180" checksum="7B255FA37112E9FA" modified="2011-06-28" version="1">MSFDADVIIVGGGLSGLSAGITAVREGLNVILLERGEYSGAKNVSGGRMYVHALKRLIPDALERAPLERPITKETFEIFCDKNRKLTFSFEEKDKKNSFSVLRSKFDRWLASEAENLGLLISYSTLVLNAYREGEGITLETNRGTLKAPLIIDASGVTSIVFRLLGLRRFTPDKWMLGVKEIIKTDVNLPENEGEVRTIVGAIKGVKGGGFVYTNKDTLSVGMAVTFDSLPKSEFPAKDLVEAFRERIGIEGEILEYSAHAIPYYGFKNLPPLYDKNLIAVGDSAGFLINDGFTIRGMDLAIGSGMIAGLAAKKIVESRNFSNTEVYYEMLKDSFVLKHLELAYNRFELINSPSTLSTYPEILCNVLSDMFTVEENRDTLINDVLYRLKEKGIPLSQAVRDLWKAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N2</accession>
    <name>F4B5N2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Molybdopterin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1573</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776371.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N2"/>
    <dbReference type="STRING" id="933801.Ahos_1573"/>
    <dbReference type="GeneID" id="10601074"/>
    <dbReference type="KEGG" id="aho:Ahos_1573"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_2"/>
    <dbReference type="OrthoDB" id="23466at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2070.10">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050612">
        <property type="entry name" value="Prok_Mopterin_Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742:SF9">
        <property type="entry name" value="TETRATHIONATE REDUCTASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43742">
        <property type="entry name" value="TRIMETHYLAMINE-N-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="704" mass="79967" checksum="68E88C63269DEA55" modified="2011-06-28" version="1">MAFLRCYMCKNACGIIATVEGKVVRVAANRNHPQPGICGRGAAGPYLLTHPDRLKSPLTREGDQLVPTSWEKALDEVTKRLKELLDEGHPEYLAITYHDYGKELLERFAALYGTPNLIGHESVCHGPRTVAAELVLGAEGPRSIDPDYPNSKFVVFIGRNPLEGIVPDIVRRIEEGRKNGMKIAVIDPRKSAIAQRYADRWIPIRPGTDTAFLLSVIYYMIKNGMYDEDFLKMYSNASLLVYEDDLSPANEYSDKLIYEGQKDGRRVATAFYLLMKEGEKVYPRLTYITGATYDDVKYIAENLWENRPSAVIDDGWHTSFSTDSTYTWMSAFIINAMIGNLDKKGGLIFSKKPRIKLYEENKAKVKRIDKIRYPLTYAAFQEVYRAILTGSPYPIKALMVVGTNLDGRDPNSDLVRRALSKLDFLVVVDVMPSDVTDYADVVLAESTYLERDELPLPVGWTLEAWVDIHQKAVEPYYDTKPLWWILLELERRLGLANDTFDSLQDMILSKLGINKEELYSKGCVKIPTEIYEVYPYKKELNTPSGKVEIYSTILHEHGYYPLPTYIEKNVMPREDDEFYLTSGHTLYHTQDSITFDIPTLIKLAPENPVTINKKRAEKLGIKDNDEVELISLTTGQRVRCKVRVTDDIREDTAFTYFGFGRHSKGEKFAYGHGFDVNSLISDQITDPISGSIAQSLNIVKIRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N8</accession>
    <name>F4B5N8_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775169.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N8"/>
    <dbReference type="STRING" id="933801.Ahos_0362"/>
    <dbReference type="GeneID" id="10599803"/>
    <dbReference type="KEGG" id="aho:Ahos_0362"/>
    <dbReference type="eggNOG" id="arCOG00729">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_5_0_2"/>
    <dbReference type="OrthoDB" id="168412at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="PIN_Pae0151-like"/>
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    </dbReference>
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        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="130" mass="14934" checksum="85C9047D8176BECD" modified="2011-06-28" version="1">MSYVFDSSSIYALLTKGKIELLGGNYTVSLAKFELGNIVWKEVLLHKRISREEGLRLLSFISKVLNTMNLEDVNCKETEEIALDYGITFYDASYVWLVRKLDLPLVTEDSKLRKAVERELKVLSVDEIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R7</accession>
    <name>F4B5R7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775198.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R7"/>
    <dbReference type="STRING" id="933801.Ahos_0394"/>
    <dbReference type="GeneID" id="10599834"/>
    <dbReference type="KEGG" id="aho:Ahos_0394"/>
    <dbReference type="eggNOG" id="arCOG00818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_2_1_2"/>
    <dbReference type="OrthoDB" id="28233at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16320">
        <property type="entry name" value="MraZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035642">
        <property type="entry name" value="MraZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF7">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, SPOVT_ABRB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="77" mass="8762" checksum="633F21BA3903CE3C" modified="2011-06-28" version="1">MGYTVTIDEKGRIVIPKEIREKLNLKEGSKVEINLEKDGRIIINVKRLTVDDIYGIAGKESVKIEEIEEALSFEDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S0</accession>
    <name>F4B5S0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS607 family transposase TnpA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S0"/>
    <dbReference type="KEGG" id="aho:Ahos_0397"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_4_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="2"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="103" mass="12053" checksum="D150297F0F9AACC6" modified="2011-06-28" version="1">MREWVKKTFGEVSVVKIKDISSGLKEDREGLKKLLAKRRQIDVVVAYKDRLTRFGFEVKLFKAYGVNVVVAFQDDKDYMQELMEGFAEIVKSFAPTVKSMRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S4</accession>
    <name>F4B5S4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase II (DNA polymerase B2) amino-end (Dpo2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775205.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S4"/>
    <dbReference type="STRING" id="933801.Ahos_0401"/>
    <dbReference type="GeneID" id="10599841"/>
    <dbReference type="KEGG" id="aho:Ahos_0401"/>
    <dbReference type="eggNOG" id="arCOG07300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2550237_0_0_2"/>
    <dbReference type="OrthoDB" id="43971at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="9069" checksum="6A5DAF8C3C8C37F3" modified="2011-06-28" version="1">MGRTQPSYTYSLQREIEKLERILSRASPHLLPILERAKGKIRYFQNASYDEDLSPSELLFLALLSELAEGCKKWLKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S7</accession>
    <name>F4B5S7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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                <person name="Prangishvili D."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRM5/TYW2-like methyltransferase" evidence="3">
        <location>
            <begin position="40"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="157" mass="17294" checksum="721A28CE5B7C9B6F" modified="2011-06-28" version="1">MIVNLKPFVNLPAKVILLRRSDFAAFYQVVIENIYSPLLSNIKEGDVVIDAGANIGLFSILASFKVKDKGKVIAIEPEPNNLKILRQNVELNKLNNIIVIPKALYDKPGKMVSIKGEGVGAYVSEDGEGLVETTTIDKIVEETGLKPQNTKNGYRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B606</accession>
    <name>F4B606_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B606"/>
    <dbReference type="STRING" id="933801.Ahos_1626"/>
    <dbReference type="KEGG" id="aho:Ahos_1626"/>
    <dbReference type="eggNOG" id="arCOG03778">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485919_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="19843" checksum="4372FF0F638B7C70" modified="2011-06-28" version="1">MKYMNPRYLRQGNYVSLIASLIIFFGLIYLEIEGISYALSSTFLLLMWIVWVLALPSFTYYHLTRLEKPKIMDYFAILAIIITLAGLIIATFLREFIGIEIIVAGYTFEPIAGISIYLTANKINKIFSSLFFWGAVIFTAGLPLYLVNFGYVSIIGDVIKMLGIVGLLSLSRKVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B654</accession>
    <name>F4B654_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ppx/GppA phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0454</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775258.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B654"/>
    <dbReference type="STRING" id="933801.Ahos_0454"/>
    <dbReference type="GeneID" id="10599894"/>
    <dbReference type="KEGG" id="aho:Ahos_0454"/>
    <dbReference type="eggNOG" id="arCOG05138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025908_4_2_2"/>
    <dbReference type="OrthoDB" id="10802at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24052">
        <property type="entry name" value="ASKHA_NBD_HpPPX-GppA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050273">
        <property type="entry name" value="GppA/Ppx_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005">
        <property type="entry name" value="EXOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30005:SF0">
        <property type="entry name" value="RETROGRADE REGULATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx/GppA phosphatase N-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="420" mass="46400" checksum="B43E5F07EAF0A11C" modified="2011-06-28" version="1">MLSAVIDAGYNSFRLSIYDVFQNNTFRLLGSLKYFVRIGEGIKEGSKISDDKIKTAEQAFIAFKNLINAKGVKNVKAVGTSAFRYALNGKEVSDKLSEILGEDLRIISGEEEGRFSATGVINTLPITEDAIIFEIGGGSLEIAKIEAGNITKVYQLPIGALKLATYSEKEIRKIVSDEISTISITPSKLVVGSGGNMRALAKLDEKISGFPSDSIHGYVVPSSQISKYAKVLFSLDIEERSALPGISKERAYTIHSGAVIIDELLQHFNADKVMVSAFGMREGVLTDGVKLDRNKWLEAIAFYHYLDPPWDVFYDAEKKVKGEMGFYVGSAAFISSVFKMSEYLNPYDACYRMTRTAIIPGFTQKEILTIGLICKAAKGKIKKKNIKSVGLDFKKKELYEYGKIVKEIVETYPLGVRWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B665</accession>
    <name>F4B665_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775269.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B665"/>
    <dbReference type="STRING" id="933801.Ahos_0465"/>
    <dbReference type="GeneID" id="10599905"/>
    <dbReference type="KEGG" id="aho:Ahos_0465"/>
    <dbReference type="eggNOG" id="arCOG02727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754697_0_0_2"/>
    <dbReference type="OrthoDB" id="35872at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd21132">
        <property type="entry name" value="EVE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
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        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002008">
        <property type="entry name" value="PRK00809.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39661">
        <property type="entry name" value="UPF0310 PROTEIN MJECL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39661:SF1">
        <property type="entry name" value="UPF0310 PROTEIN MJECL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="1">
        <location>
            <begin position="2"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="146" mass="17156" checksum="78B641004D2D4168" modified="2011-06-28" version="1">MTYWLIPIQEDMWDTIRYEGVYGYKQDLSQYIAKGDYLIIYVSKYYAKVYGGKIVGIVKVLTEWYHDETPIFPEEKVRNRALFPYRVRVEPVITGVCDFKSILDKIVFIEDKAQLAKYLRNAPANLKRPIPEQDAKLIEECLNGHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B683</accession>
    <name>F4B683_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Citrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0483</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AEE93371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CitMHS"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
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            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
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            <begin position="305"/>
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        </location>
    </feature>
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            <begin position="343"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="8">
        <location>
            <begin position="23"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="408" mass="45036" checksum="583368E821EBD75B" modified="2011-06-28" version="1">MMNYLSLAVMIITYSLIATRGLTKIPPWASMFFGGVLMIILGVLTPEKALSAINLDVILFLITIFTFASALEVSGFLKYVAYAIISKFKRPDKVLFSVLVTSGLLSNLVTNDGLSASWTPVILEMKKEMKIDEKPFLYALAFGVTIGSVMFPTGNPQNLLIALDSGIKNPFLVFIEYLSIPTLLNLVLTYPILRLMFRKYLPNLTFNPDKIEIENKRLAYSSFILLLVTIVLFFTLSYVGIDILLGSLTTSSILLLISPERRDIVRRIDWSTIIFFIGLFIFTEGILCGGVIAEMSKFLPPPTSILLIFISSILLSQILSNVPLVAIYIPIMFEYHSTTIADWLALAAGSTIAGNLTLIGAASNIIISEASENRGGKGFGFFEFIAYSLPVLLLNSSILYLFLLLEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B4</accession>
    <name>F4B6B4_ACIHW</name>
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        <recommendedName>
            <fullName evidence="6">Cytochrome b558/566 subunit A</fullName>
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    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B4"/>
    <dbReference type="STRING" id="933801.Ahos_1656"/>
    <dbReference type="KEGG" id="aho:Ahos_1656"/>
    <dbReference type="eggNOG" id="arCOG06015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_575729_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1190">
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cyt_b558/566_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03154">
        <property type="entry name" value="sulfolob_CbsA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EB_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EB_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="468"/>
            <end position="490"/>
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    </feature>
    <feature type="domain" description="Cytochrome c-552/DMSO reductase-like haem-binding" evidence="8">
        <location>
            <begin position="93"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00887"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE94534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="492" mass="54235" checksum="1956BFA21C2E3FE8" modified="2011-06-28" version="1">MNIFYIVFFIKIIITLNYLIKIISPTFYPYYVEERFSSCEFKMRKKIPLLIALVAMLFSIMLAFENVPLAQSSPTIVAYKVVGSANLANPGSCSFWSDIPWVNLSLTANIPNAPTSGLTHYILVKAAWNGSDIFILVKWPAPNPAFGAWSAAAAGLYPSATGPGLFRIIELMPGTTYKLLTNCTSYVSIVNGKSESGRLAFSYKGVTVLATNDSQIQVLSNGTILFYHSPRPMEYIFYCNGLFYGYYTNSTWYYPDRVAMMWYMGTGTPTMDGMNIGGKYAGQVYDGYNLSVAGGSFKMPGGSANIWMWVSGATWNNATYDPAFKVHLWANESLTGLPYTDPDCGGFAVPLYTNNTNMYEVDCAGIWYNPVQSSGLNGSLFMIRTGAKLCNGYWTVEYVRSLTVPSNIAKYEPQIALNTTYYVAFAVWQGREGETLFDKSIVTPFVTLALSTASHPTPPPPLVISPTLMTVTVVGVVVAVVALAIIWIVYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C1</accession>
    <name>F4B6C1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-like toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C1"/>
    <dbReference type="KEGG" id="aho:Ahos_1663"/>
    <dbReference type="eggNOG" id="arCOG04502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136715_0_1_2"/>
    <dbReference type="OrthoDB" id="198094at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039018">
        <property type="entry name" value="VapC20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188:SF1">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="18837" checksum="6B6DF94B0389BD89" modified="2011-06-28" version="1">MIILDTSFLYSFLNKNDANHNKAVELMNKIISRKFGKPIIFEYVIDELFTLMINKQPFSYIEKVIDLILQDVEKNIFQIITLTDRENSLHNIIRLFKKINEDPNRKRKLSFTDCAILFAMIQNDIFYLASFDSGFDGTLEKIEDGIYALSSDRTLNLKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D3</accession>
    <name>F4B6D3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776468.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D3"/>
    <dbReference type="GeneID" id="10601176"/>
    <dbReference type="KEGG" id="aho:Ahos_1676"/>
    <dbReference type="eggNOG" id="arCOG04029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2475811_0_0_2"/>
    <dbReference type="OrthoDB" id="379670at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="87" mass="9758" checksum="8F627B945D9206CE" modified="2011-06-28" version="1">MAIRKYAPVTCFKGDKPYVKLTSVNGDTLDIDLRVDTGFEGTILLDSSTYSKFCVGELPESLWPRFKTLNGYLVMRASKAMLNVFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E1</accession>
    <name>F4B6E1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcvH</name>
        <name evidence="5" type="ordered locus">Ahos_1684</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="3">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776476.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E1"/>
    <dbReference type="STRING" id="933801.Ahos_1684"/>
    <dbReference type="GeneID" id="10601184"/>
    <dbReference type="KEGG" id="aho:Ahos_1684"/>
    <dbReference type="eggNOG" id="arCOG01303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_2_2"/>
    <dbReference type="OrthoDB" id="9810at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715:SF3">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM H PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="4">
        <location>
            <begin position="29"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="3">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="15940" checksum="75E3F38400E39FCA" modified="2011-06-28" version="1">MNISGFEFPEELLYDEEKHVWIKKEKNNVISIGITSLGQYMAGKIFQVSTKEVGEKVTPKSTLFSIESAKWVGKFRLPITGEIIAVNENVIKDPSIINEKPYDAWIIKVRVESIDERRFKTINEAYEKFKEEAEKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F5</accession>
    <name>F4B6F5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peroxiredoxin family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F5"/>
    <dbReference type="STRING" id="933801.Ahos_1698"/>
    <dbReference type="KEGG" id="aho:Ahos_1698"/>
    <dbReference type="eggNOG" id="arCOG02064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094970_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032836">
        <property type="entry name" value="DsrE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655">
        <property type="entry name" value="CONSERVED WITHIN P. AEROPHILUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34655:SF2">
        <property type="entry name" value="PEROXIREDOXIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13686">
        <property type="entry name" value="DrsE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14411" checksum="9CB0867CA437757E" modified="2011-06-28" version="1">MFSGTIDKLMPVGILTSGAAAAGYEVNLFFTFWGLQALTKKSIGQPQPIDKNYEQFGPVMMQRMQEMKYPTWDQLIMQAKEVGEVKVYACSTTMEFFGLKREDLADFVDDVVGVATFLDRAEGGTTLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G4</accession>
    <name>F4B6G4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776499.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6G4"/>
    <dbReference type="STRING" id="933801.Ahos_1707"/>
    <dbReference type="GeneID" id="32173771"/>
    <dbReference type="KEGG" id="aho:Ahos_1707"/>
    <dbReference type="eggNOG" id="arCOG04507">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968243_0_0_2"/>
    <dbReference type="OrthoDB" id="35110at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007029">
        <property type="entry name" value="YHS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04945">
        <property type="entry name" value="YHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YHS" evidence="1">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="58" mass="6841" checksum="890DB6DCE3E4A261" modified="2011-06-28" version="1">MKVICPVCNRLFEAECSPYKEMYNGIVYYFDTEICQLAFRQNPERFVYNCKNSGSQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H7</accession>
    <name>F4B6H7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776512.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H7"/>
    <dbReference type="STRING" id="933801.Ahos_1720"/>
    <dbReference type="GeneID" id="10601220"/>
    <dbReference type="KEGG" id="aho:Ahos_1720"/>
    <dbReference type="eggNOG" id="arCOG01424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965117_0_0_2"/>
    <dbReference type="OrthoDB" id="28470at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019323">
        <property type="entry name" value="ELKS/CAST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38753:SF1">
        <property type="entry name" value="SLR1441 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10174">
        <property type="entry name" value="Cast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="351" mass="39191" checksum="675FB16B101ECA57" modified="2011-06-28" version="1">MSGKIADEILNNPQLLSALADKIYEKLKDEIVIKKLEETIASVKALQEEIKKQGEAIASLQQEIKSLREASNKHGEAITTLQEAVKKQGEAITALQEAVKKQGEAIASLQETVKKQGEAITALQQTVNKHTEAISSLQEAVKKQGEAIMSLQETVKSLQETVNKHTEAITALQEAVKKQGEAIASLQEAVKSLQETVNKHTEAIEGLQKAVRKLQRAVMKLSIEVGSFTNRAGKGMEKTMLKLYRKALELHGVDPKKVVHGIIKDEEGLIEKGKVFEVDFYETNDYVYVFEIKNFADKGAYDQIIIRKKLFSAKYKDKKIKIFLVANFVDKKIKKKLEEEGVEIITSHVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J3</accession>
    <name>F4B6J3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">P-loop ATPase/GTPase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775319.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J3"/>
    <dbReference type="STRING" id="933801.Ahos_0515"/>
    <dbReference type="GeneID" id="10599956"/>
    <dbReference type="KEGG" id="aho:Ahos_0515"/>
    <dbReference type="eggNOG" id="arCOG04328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084678_0_0_2"/>
    <dbReference type="OrthoDB" id="39107at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="263" mass="30084" checksum="3B67CFF748901B39" modified="2011-06-28" version="1">MKILVNGLLPYDSGKTTFSLMLIRELKAVGIKLFPLKPVAGHNAWYSFNTLLRSYDLGILAGNDALKYYDETNEDIRKINPFAALLSPVDLEILNFSVEYYNSLMTNGFPVMIRISCGNNDKYYATETHLIPKTIKGVLQQIYEKFKPEIVSSNELRETINNSWYIAESCIQHRDDEIIESYNDASAPIPSAINVDHVFLVSPAKAFVIKGEEFRKVISLFSLPPWIIRTSNLIKYLKIEKSFDLEVFTSKNDKIIDYLIHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J7</accession>
    <name>F4B6J7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Replication factor C large subunit</fullName>
            <shortName evidence="7">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfcL</name>
        <name evidence="10" type="ordered locus">Ahos_0519</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775323.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J7"/>
    <dbReference type="STRING" id="933801.Ahos_0519"/>
    <dbReference type="GeneID" id="10599960"/>
    <dbReference type="KEGG" id="aho:Ahos_0519"/>
    <dbReference type="eggNOG" id="arCOG00470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027255_1_1_2"/>
    <dbReference type="OrthoDB" id="8658at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003226">
        <property type="entry name" value="PRK04195.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003229">
        <property type="entry name" value="PRK04195.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="40"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="408"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="450" mass="51254" checksum="1045A24E27863D09" modified="2011-06-28" version="1">MTLPWIIKYRPKSLNDVENQDDVKQELKRWIESWLSGKPENKALLLYGPPGTGKTTLAQALAHDYGLELLEMNASDSRNLEAIKNIAQRAAVSGSLFGTKGKLIFLDEVDGINARQDMGAIPAIAELVQKTRYPVLLAANDPWDPSLRELRNLAKLVEVKKLGKYALRKILGKICTSEKLQCDDDALDEIIEISDGDARYAINLLEATAEGFKKVTVESVKEFARRKESELDPFETVRGVFWAKYAWQAKNAVTSSQVDYDLLMKWFSENIPVQYDNMEDVYRAYDALAKASIFLSRAKTSSWDMLSYTFDMMGPGVAMAEIEKQNPGWKPKWRKYQFPQTIQLLSKSKSNREIRDKIVSKIAGKIHSSEEKTLNDVYPLFLQFYKKYEDKLSKDMDLSPDEINFLNEISGKSQGAEEEKEETTYSKASATSKRYYSSGGRRYTTRKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J8</accession>
    <name>F4B6J8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="4">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">moaC</name>
        <name evidence="6" type="ordered locus">Ahos_0520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775324.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J8"/>
    <dbReference type="STRING" id="933801.Ahos_0520"/>
    <dbReference type="GeneID" id="10599961"/>
    <dbReference type="KEGG" id="aho:Ahos_0520"/>
    <dbReference type="eggNOG" id="arCOG01530">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
    <dbReference type="OrthoDB" id="10067at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01419">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008999">
        <property type="entry name" value="PRK12343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF29">
        <property type="entry name" value="CYCLIC PYRANOPTERIN MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin cofactor biosynthesis C (MoaC)" evidence="5">
        <location>
            <begin position="6"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="66"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="149" mass="16540" checksum="F398955E3CFF2EE4" modified="2011-06-28" version="1">MTEARMVDISEKQPVLRIAVAEGYIKLKKETIDKIKRGEIEKGDVIAVAKVAGIMAAKKTPEILPMCHPIPLESIAVDVELEEDKVKVKTEVKAHYKTGVEMEALTATSVALLTIWDMVKKYEKNEEGQYTTTQIDGIKVVNKLKISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N1</accession>
    <name>F4B6N1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin-guanine dinucleotide biosynthesis protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775357.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6N1"/>
    <dbReference type="STRING" id="933801.Ahos_0553"/>
    <dbReference type="GeneID" id="10599996"/>
    <dbReference type="KEGG" id="aho:Ahos_0553"/>
    <dbReference type="eggNOG" id="arCOG00532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068199_2_2_2"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052539">
        <property type="entry name" value="MGD_biosynthesis_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072:SF1">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin-guanine dinucleotide biosynthesis protein B (MobB)" evidence="1">
        <location>
            <begin position="4"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="164" mass="18734" checksum="1BEEE5E1BF108FDF" modified="2011-06-28" version="1">MTCVFQVVGKKDSGKTTAILEVIRELKSKRKDLIIAVIKHSHHIIDDENKDTFKFKKEGADLVVFHSNDCALFFDCKEFDYLSLFPADVILIEGFKDLNLGYKFEIRSPEEAKEIAEKISKKAEECVSYPNLEINGAKAQKSLLTSFLYTMMKKYGIKEIKIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R2</accession>
    <name>F4B6R2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R2"/>
    <dbReference type="STRING" id="933801.Ahos_0584"/>
    <dbReference type="KEGG" id="aho:Ahos_0584"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_4_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF31">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN YCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="22"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="29481" checksum="F9CFDCD5D3FB14B1" modified="2011-06-28" version="1">MSDMFVILDDAKIYYEIRGEGKPVILIHHLAGSYKSWSDIAYQLSQKFMVISYDLRGHGRSSILPYEYRIEDHSKDLKGLIEYLRLDNPIIIGHSLGTLVAIDYALRNAVDKLILIGALYKAPNPEPYEKYVSVALNFGMEALAEYRKTIGDFADSLVLNPVAWRKLLEVYNENNPLGYKYAVEGLLHARDYSKDLNKIDVKGGTLLIYGSEDKLKQNLNTMLTIPNSSYRIPNGYGHFLNFEAPDELYASIIDFLVGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S4</accession>
    <name>F4B6S4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated protein, Cas6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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            <end position="119"/>
        </location>
    </feature>
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        <location>
            <begin position="137"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="287" mass="32133" checksum="C9E712C359CA8191" modified="2011-06-28" version="1">MLALVKTTYNVTPLTDVVLPSPSSKVLKYLILSGKLFPSLANLVKSRDKQKPFFISNLGYGDVRLISDGSEVIKINANSRLKATLSFPFLDGIQNEITEGVYETPYGKFSFLLDSIEIVDIKSLKNVNNYENANIYVKFLTPTLLSSKILLPPSLASKYKQVNSGFSLLPSIGLIIAYAYRNYYAILGNTNGEEYASRAFKLGVLINAFTKIVGFNLRPKTVIIGRDSKKRLRETRGTIGWIEFDVVHDKFKRLAIEYLLIASYLGLGRGRGIGLGEIKFELKRRKD</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
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        <source>
            <strain evidence="2 3">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="45"/>
            <end position="66"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="109" mass="12713" checksum="FD0E16A89B9C31FB" modified="2011-06-28" version="1">MDRVKVLWFIIILGNLFDYVVTLLFSYLGLLYMDRNVFIGYDTSFLHVLLTLTGEKLLFLSGVYWFSKLFDYLKISKYKWIGLLPFTIITILVVSYLAIGIIVIFLLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W6</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="STRING" id="933801.Ahos_1783"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830598_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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            <begin position="201"/>
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        </location>
    </feature>
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        <location>
            <begin position="322"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="342" mass="38236" checksum="9359EF8E42D25FD1" modified="2011-06-28" version="1">MRFSNVFMLEIKKLLPLFFLVLVSMAVTLGIAQLPSSFDVIGAISYSSYGVEIGGYGFNFCGQGVPLTVNITIDCFHKIIKSENGTFLVYIPLIFPKDSNLIINITSGKENYVVSKVLTRNFTTFVYAPASYFSINYNNISMIYVKGETVIATSLKCIYVNGEKMNVNSTIFVINSDNVKPPFGNYALTDLQVIKDEEYCIISLLGVNMGLILLTAYYYLSVFPRRQLDLIFRLIGVGKIYLSKTISMLVFTLLTSLPSLIIFALLKGIFLSSIVPYILVELVYFLAVLGISPLVSSKGLVYSSITLAGYLLNLVFYNEETLSLAFLILPLIGYIKLRWVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X1</accession>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B6X1"/>
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    <dbReference type="KEGG" id="aho:Ahos_1788"/>
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    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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    </dbReference>
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            <end position="69"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="105" mass="11465" checksum="6C2472A9D8D661BE" modified="2011-06-28" version="1">MSSILCSKLHSAVEYGNLLQVKTLLALHCNPNVKDKSGYAPLHYASESGHTDVVKVVLEHGADPNIKDKFGKSPLLYASKKGNNEIAGKSRYERVRCVIITAILT</sequence>
</entry>
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    <accession>F4B6Y5</accession>
    <name>F4B6Y5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776593.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y5"/>
    <dbReference type="STRING" id="933801.Ahos_1803"/>
    <dbReference type="GeneID" id="10601303"/>
    <dbReference type="KEGG" id="aho:Ahos_1803"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="OrthoDB" id="41609at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="178" mass="20586" checksum="65813D767538BCCD" modified="2011-06-28" version="1">MEEKLSSPKIDRKAYIRARIIETLDDLDVGINMWLAGRTRNSAGKIFNAVKALLSALVTKNLDKLSAKDEWYAKRGYTAPTHALKGISIDLAKLGYRDVENITNLAFLLHDYQYNGFDPDFSKYKNKEEVLHDIITLARVILDNIKKEWFKEEWDEKLDVLYSIVTSDLERMEKNSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y7</accession>
    <name>F4B6Y7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyltransferase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y7"/>
    <dbReference type="STRING" id="933801.Ahos_1805"/>
    <dbReference type="KEGG" id="aho:Ahos_1805"/>
    <dbReference type="eggNOG" id="arCOG01204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136008_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449:SF3">
        <property type="entry name" value="POLYMERASE NUCLEOTIDYL TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="139" mass="16728" checksum="DDC27F1F59720792" modified="2011-06-28" version="1">MRNVSDAWKKYSLFSDLLQIYKEEEEQFKDYVNFLCNKNYTVILFGSRARGDFKIYSDYDLLVIGEDFPKFPPTDAIELHFYKKEKVDKEIAEFNTVIIDAFYEGKLLCDKLNIYEEKRRKVLERIKGLKRVKDGWIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B710</accession>
    <name>F4B710_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_0605</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775409.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B710"/>
    <dbReference type="STRING" id="933801.Ahos_0605"/>
    <dbReference type="GeneID" id="10600052"/>
    <dbReference type="KEGG" id="aho:Ahos_0605"/>
    <dbReference type="eggNOG" id="arCOG01181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_2"/>
    <dbReference type="OrthoDB" id="50101at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="12">
        <location>
            <begin position="68"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="289" mass="33472" checksum="5A123C7ADFF394EE" modified="2011-06-28" version="1">MPSLTLAEKASLVLPEDYLVLKTFVELKDKYEYLTEEELKDRLNFTDTELRISLEKLRNLRAISWDIITGKRAYKITFTGLDILAIKMLYVNKILNRLGLIIGEGKESNVYYGYDFDNNTIIVKFHRVGNSSYKNARKLRGYREKRSWISITLDNAKNEYIALKCLSENLAKVPKPLGYAYNAVAMEYIEGPSLLKAELNNPKEVLDSIIGSLRIAYLYCNKMVHGDLSPYNIIIGDNESPYIIDWPQWKQDDDNLLNRDINNIISFFSKKYNITEDLNYVMDFIKGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F4B745</accession>
    <name>F4B745_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="5">3.13.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="5">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ahcY</name>
        <name evidence="10" type="ordered locus">Ahos_0641</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 7">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="5 7">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 8">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_2"/>
    <dbReference type="OrthoDB" id="8479at2157"/>
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        <property type="reaction ID" value="UER00076"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5 7" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="9">
        <location>
            <begin position="182"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <position position="124"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="147"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
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        <location>
            <begin position="148"/>
            <end position="150"/>
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        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <position position="177"/>
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        <ligand>
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        <ligand>
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        <location>
            <begin position="213"/>
            <end position="218"/>
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        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <position position="234"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
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            <position position="269"/>
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        </ligand>
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        <location>
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            <end position="292"/>
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        <ligand>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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    </evidence>
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</entry>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Shah S.A."/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7153" checksum="5773F193523C4D92" modified="2011-06-28" version="1">MEELIKKAEEKGIDVEDLIISAISRIDPQDSIKLRFEIAKRFLSEAEDYLSKGDIIQSSEKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="263" mass="29884" checksum="54CD0B69AF0F7FB6" modified="2011-06-28" version="1">MKLMYRNEIVINANRSSVLGVLTNPFFISGISGHVGILRVKDKQKGDYVTSEFLNSPENEFEVAYVFGTPENYHVYLGHMIGPEVSLREVKYKGETSDGKFKWEVSFFLTPINDNKTRVSIAVDAKYKTSTLARIFGRSEFDLATHIVEGHFIPFFKFYFKPSEEIEVNKIEIESEEGEANKVIAKFKEIVPRLEAGLIKISGKNLECLVIVINKDIKRVTCLVGNDVRTGSEALSLLLLYNGELKMKAYQLQLEDLIEKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D2</accession>
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        <submittedName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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            <strain evidence="2 3">W1</strain>
        </source>
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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        <source>
            <strain>W1</strain>
        </source>
    </reference>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="515" mass="55286" checksum="C384AA1CF0289031" modified="2011-06-28" version="1">MPLRKELGFMELVILGISGAVGTGVLFSSAGMSALAGPGVILAWVLGSIFYTFVGLTYVELSKVYPEAGGPSRYAIYSHGKVTNLINAMADLIWYLFIPPIEALAVVEGLDYFYPSLISSSGVPTTEGVLIGVAILLLFVPFNYFGVKFFGKSTLIGGGVKLILYLAVALGVIALSFIPNNFTAYGGFLPFGFAGVFSAIPLAMFAFGGIRVIPDYAEEAKTEKLGSAILITILGQSLIYVIFSIAFVASLNWNKLGIKQGCWSSLSSLPGNPFIDIASSLHSSLLLLLTLIIGIIGPFITGYIYLGAGTRVLFAMGRSGYVPSSMKELHERYSIPYWSLIVFAIVGSVVTYISSPLPTIYGLITDSVVAGYIGFSVNPVVMGSLWRRGVIKNKLTPVVSPLAFISSSLIVFWSGWPLVPYAVILLTAGSLIFGAIYKVKEDFVKSLWYIGDIAFLTIMTYIGSDGALNLINFYEASAIVSVISLIFYFLGVQSSNISITTTRNNSDKTNNKEVS</sequence>
</entry>
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                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    <sequence length="180" mass="20300" checksum="F1061FE4F095CE5D" modified="2011-06-28" version="1">MLKALIFLLIVLALSSLVSYSYYSNLSFKVGYSPPNKVILEIYSVVNNPVNISFNNITQEIYIRTTYNLTGVNISLVHTVIPNCPKSKCCYIINNEYLMYYNSTFLILIMNNSKEIGYIKIIVCKPENLTTPSLTNLNDNGTLPSKAKDGSSIYDYIIVLLIPILSVTVSILLRVIKRNK</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="17"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="184" mass="20580" checksum="16F147EA7C2F9942" modified="2011-06-28" version="1">MREFYIVNDVIDGIPLSLVSHPGLFSKKKIDLGTRTFLENLIIPQQGTVVDLGCGYGVIGIFIALKNENLKVYMLDVNPLAIATAKMNVERYNLQNRVVVMKSDVLSALQEKVDAIYSNPPLSKGVDFLQKFAEQSAEKLKKGGFIQMVVYKGENNVLKIFSNYFSDIKVIKRSKGYSIIVVNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M9</accession>
    <name>F4B7M9_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">Polymerase nucleotidyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776691.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M9"/>
    <dbReference type="STRING" id="933801.Ahos_1903"/>
    <dbReference type="GeneID" id="10601402"/>
    <dbReference type="KEGG" id="aho:Ahos_1903"/>
    <dbReference type="eggNOG" id="arCOG01200">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830481_0_0_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="16770" checksum="09EF4DD43B8670CF" modified="2011-06-28" version="1">MVEDLMRRINEEYERKFSDFLKKLISAYLDSTIVLFGSRARGKNRPNSDFDIIVFVSCENPLHKSIEIYQTTRPDFPVDIIVMKPEEVNDRNLKLTHMFYYGYKILYDGLNLKEKIERMMRENVDKLKDIVIYDDPKYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P4</accession>
    <name>F4B7P4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Band 7 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776706.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P4"/>
    <dbReference type="STRING" id="933801.Ahos_1918"/>
    <dbReference type="GeneID" id="10601417"/>
    <dbReference type="KEGG" id="aho:Ahos_1918"/>
    <dbReference type="eggNOG" id="arCOG01918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914065_0_0_2"/>
    <dbReference type="OrthoDB" id="40668at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37826">
        <property type="entry name" value="FLOTILLIN BAND_7_5 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37826:SF2">
        <property type="entry name" value="ZINC-RIBBON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="1">
        <location>
            <begin position="41"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="312" mass="34014" checksum="0EFD06996E8AA072" modified="2011-06-28" version="1">MSISFRGQVVSTELENGASLMTQDTIIFRYPKENITSKSLFIVQPTERCIVIIQGQIAADLPPGTHNIQSPANPVSAFLSKFRYQSLPYDTVVYFVSMTRHEVRVSGISQTDDLVPLEYEVAVYFRVQNPSLLVTNVQFGSQYFKDADLAAYINPIVDQDVSQVLNNVKLVDVFKKFADITTAVTAGLKTFLGEIGIDLISVRITKLIPQDPELRRILQLRDLGIEINDAIRLGLARVLAERSDPSSVNMALGVPYFPQLSTIVIGGGYLPYVGGGVTPTPSHETPGGVVGTPAPQMPTVQSVLKNVFGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7P7</accession>
    <name>F4B7P7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776709.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7P7"/>
    <dbReference type="STRING" id="933801.Ahos_1921"/>
    <dbReference type="GeneID" id="10601420"/>
    <dbReference type="KEGG" id="aho:Ahos_1921"/>
    <dbReference type="HOGENOM" id="CLU_2079287_0_0_2"/>
    <dbReference type="OrthoDB" id="39927at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="13315" checksum="E286193DBD1AD888" modified="2011-06-28" version="1">MKEAFLILGKDLLRVTCNYSECEWFILDQNCSLEIVNIKQKGKVKITGNAENLQVMKVLLSFLLSGKDPNDAVCIQKSGRTECIKDDEGIGIMEGKWSGRIFFNEEELENLLKNLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T3</accession>
    <name>F4B7T3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776745.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T3"/>
    <dbReference type="STRING" id="933801.Ahos_1957"/>
    <dbReference type="GeneID" id="10601456"/>
    <dbReference type="KEGG" id="aho:Ahos_1957"/>
    <dbReference type="eggNOG" id="arCOG03672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_489686_0_0_2"/>
    <dbReference type="OrthoDB" id="34640at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="556" mass="63457" checksum="1D94359D76280B35" modified="2011-06-28" version="1">MHIIKLVIVILIFLSIVNYVSAYLQCSNTYILNITSPSAFIFDIEGENVNITILRIGNSSCQQLIYMDYTKNNLTSGLLLIQGKYEVVVKSIGQFIFHYYIIKLKCIQKALCPSFLFATNKSVLIPIPDLTTPEALRAFIVSNVSLTFEIIQCNKIIKEYENTTIVFSKYSYVYFKNPKNIFIKICVYKDSLFYLSYKVIPCFINPYLKLSTPSSEGIASYGILNETKTTKPYLIKTTSILGKFNISCILAYNYSQNLVPPCSASLQLNVVLKTCSQVYWLQNVLEFLTLKHEFKLADDILNITCIDSTLSNYSITSLNGYVTMVNQSGKIEYYYGNYYCQSELKYCLPLCGYLVTNVSLEKCGILVSFAVIFIKNGSSTCYKEIVFDNVLIHGKFRFAYILVCGKHYTPIGSYYDAELVFGGGGNGEITKFCKLNATLWLFYLKKNTYVTFPNYFTFGEDTAEKAVNITVNFCKYYAKLTTGKENFRCYKAYSYSIDPFHYHNISSLHNSSTKSITVITIGSTHASNPNYYTYEILIPIALILSFILYYLLFRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U1</accession>
    <name>F4B7U1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776753.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U1"/>
    <dbReference type="STRING" id="933801.Ahos_1965"/>
    <dbReference type="GeneID" id="10601464"/>
    <dbReference type="KEGG" id="aho:Ahos_1965"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="OrthoDB" id="32909at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053795">
        <property type="entry name" value="Lrs14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040939">
        <property type="entry name" value="trans_reg_lrs14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="13876" checksum="61E03D582DC8FA77" modified="2011-06-28" version="1">MEVQKFKVRLPSGKEVGLLDALLFCYDISDTDFKVLKELIKNGPKNEDELAAELKLSKASVNRSLNKLLSIGFVQRMKDQSSKGGRPRFIYSAISSDEIVNKISRDFEYCAKIFISITPAELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U4</accession>
    <name>F4B7U4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776756.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U4"/>
    <dbReference type="STRING" id="933801.Ahos_1968"/>
    <dbReference type="GeneID" id="10601467"/>
    <dbReference type="KEGG" id="aho:Ahos_1968"/>
    <dbReference type="eggNOG" id="arCOG00837">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1763764_0_0_2"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072:SF60">
        <property type="entry name" value="L-2,4-DIAMINOBUTYRIC ACID ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43072">
        <property type="entry name" value="N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="147" mass="16526" checksum="C18E1925CF1B3218" modified="2011-06-28" version="1">MKLKEVIKKSLSPFDSYYALLNLSSSKILVEKEGKEIVGFVELKIYEDVGIIFYLGVLPCHRGKGHGKALVKKAEDYFIKKGLSLSLASTRDWNKVAVSLFTSLNYKVFKDDEVSFSVIELLNAYDDNLVLCKELKKGISCESFLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W9</accession>
    <name>F4B7W9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="8">1.1.1.302</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0760</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HTP reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775562.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W9"/>
    <dbReference type="STRING" id="933801.Ahos_0760"/>
    <dbReference type="GeneID" id="10600217"/>
    <dbReference type="KEGG" id="aho:Ahos_0760"/>
    <dbReference type="eggNOG" id="arCOG01484">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_4_1_2"/>
    <dbReference type="OrthoDB" id="10178at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050765">
        <property type="entry name" value="Riboflavin_Biosynth_HTPR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011:SF7">
        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="Bacterial bifunctional deaminase-reductase C-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="216" mass="24695" checksum="0CAFDF105E214415" modified="2011-06-28" version="1">MYVIIFSTITIDGKLASKDYYSELSCKYDKVRQHILRSEVDAIMIGGNTVRIDNPSLTLKYVHGKNPIRVIISNSLNFDINYKIFNTPPQTIIYTSNNSENKDFENKIKEKGVIIRKLDNFNICNVMNDLESNFNVHKVMIEGGGKLIWSVIKENCFDELRVTISPRVFGNGVSLANGEGFLGKNSPKLDLEDVKICECKEEVHLIYKNRNKIRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B876</accession>
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        <submittedName>
            <fullName evidence="3">Oxalate/formate antiporter</fullName>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B876"/>
    <dbReference type="STRING" id="933801.Ahos_2028"/>
    <dbReference type="KEGG" id="aho:Ahos_2028"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_745192_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF304">
        <property type="entry name" value="MFS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
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        </location>
    </feature>
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        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
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        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
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        <location>
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            <end position="291"/>
        </location>
    </feature>
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        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="365" mass="39106" checksum="A665BBD0C2A5EFC6" modified="2011-06-28" version="1">MSFNSLYQYSWNALEPLLREGFNVSIVEISLGFSLFTIFSSGFQPLGGNFADEIGPRKVALLSSILSALGFLGTSFSPSVYIFFIFWSLGSIGEGILYGIASNLAVKWFKKRMAFATGLVSLGFGLGSAFADPFILMSGNFRLVTLIIGLTELIILPILSLLIEYPTLESGKSPKEAVLSRTFWLIYLSFVTATVPLLVISSSLSIIGKALPFQELSILISIFPILSGSGRPIFGHIADKLGVIKTTVIVDTIIVLGGASLLFSQIIPAVVIIGLAGGAVITLYFNVSGIVFGTRYSTVNNGILYTGKAVAGFLGSVIFNYLFLISPFLAYLFVIISSTIGAVTLYGIFHHAQPAEGSSQVRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E3</accession>
    <name>F4B8E3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps4e</name>
        <name evidence="10" type="ordered locus">Ahos_0856</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775658.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E3"/>
    <dbReference type="STRING" id="933801.Ahos_0856"/>
    <dbReference type="GeneID" id="10600324"/>
    <dbReference type="KEGG" id="aho:Ahos_0856"/>
    <dbReference type="eggNOG" id="arCOG04093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="OrthoDB" id="372073at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
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    </dbReference>
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        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
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    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="37"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="238" mass="27125" checksum="AC7F0ED6966919A3" modified="2011-06-28" version="1">MPHITRFEAPWFLKISKKEYKWTVRASPGPHPLSKSIPLSLILRDYLKVAATLSEAKKVISEGKVYVDGIIRKDYRFPVGLMDIISIPSAGLYYVMFPDKSRYISPKQISESEAKYKLVRIMNKTMIKGGKLQLNLEDGRNILVNKEDSSKYPTLSTLKIEIPSQNIISVYPLVENMYGIIIGGKNTGLHGKIVKIQRAQYKTRKYSIVTIQKDNESYETNLLNTMVIGEQNPEIKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G9</accession>
    <name>F4B8G9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775684.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G9"/>
    <dbReference type="STRING" id="933801.Ahos_0882"/>
    <dbReference type="GeneID" id="10600353"/>
    <dbReference type="KEGG" id="aho:Ahos_0882"/>
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    </dbReference>
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    <dbReference type="OrthoDB" id="35722at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="139" mass="16107" checksum="92F83BE51985529F" modified="2011-06-28" version="1">MESNTDELRKFLENKISNLKKELEYYEYLLSLLESGFSPVKGSKGSIDYIKSRKGEIIAEVYYTPPLMKIIVRRKLTLSKAYINALSKILEDSKNNDKIDYNIVFDKDDLKEISINNVNDDLCYNKIKIALQTILERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H4</accession>
    <name>F4B8H4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase, BadF/BadG/BcrA/BcrD type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H4"/>
    <dbReference type="STRING" id="933801.Ahos_2055"/>
    <dbReference type="KEGG" id="aho:Ahos_2055"/>
    <dbReference type="eggNOG" id="arCOG03659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045127">
        <property type="term" value="F:N-acetylglucosamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ASKHA_NBD_KdgK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039758">
        <property type="entry name" value="NAGK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862">
        <property type="entry name" value="BADF TYPE ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12862:SF0">
        <property type="entry name" value="N-ACETYL-D-GLUCOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="317" mass="35017" checksum="1EE81E6142786602" modified="2011-06-28" version="1">MAVDGGATKTVALLFDEKNFKIKGIGLAGSSNFTIIGVSKAEENLRKAIKEAEGDKADKAVFCLAGIGDSAEDTKRGEEMVRRIFPNAQVYNDGVGAYRATNLFEDGGVFAPGTGSVAFYQKNGELHRVGGWSWIFGDEGSASWIAKTAITYATRVVDGIEEKSELPHAVEEYFKLPFREAVIKLSKEQDKRFIASFAVIVDELAQKGDPLALRVMKEASEYITKMINRLKKEGGRVSVIGGVMNSRIIRYEISSLGVSIFYSYQAVIGGLILAGVKMRFEERDILAKELTQMVYNLPEEELKNYLFINIKSKYSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H7</accession>
    <name>F4B8H7_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776844.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H7"/>
    <dbReference type="STRING" id="933801.Ahos_2058"/>
    <dbReference type="GeneID" id="10601558"/>
    <dbReference type="KEGG" id="aho:Ahos_2058"/>
    <dbReference type="eggNOG" id="arCOG03706">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153003_0_0_2"/>
    <dbReference type="OrthoDB" id="42482at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="141" mass="15943" checksum="C83CF4F0EA5A5DD7" modified="2011-06-28" version="1">MDNLFYVDTNVIISLIENDSNYSKAERIMRLNNLVTGEVTLLELISFYSRKLKDEILAKASAKYAIRLANVKVVEIDANKLLKKAIELSPKLQLKTLDVIQIVSAILINSAVFVTFDSDILKKKEIVMRYSGIKVTDFITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I2</accession>
    <name>F4B8I2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl coenzyme A synthetase (ADP forming), alpha domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776849.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I2"/>
    <dbReference type="STRING" id="933801.Ahos_2063"/>
    <dbReference type="GeneID" id="10601563"/>
    <dbReference type="KEGG" id="aho:Ahos_2063"/>
    <dbReference type="eggNOG" id="arCOG01340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_2"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF2">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="469"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="660" mass="72020" checksum="7A785355188DAFAF" modified="2011-06-28" version="1">MSLDPLFRPKSIAVVGASRNREKIGNIILRNLIATYRGKIYPVNNKAEEIEGMKSYKTLKEIPGDVDLAIISVPRQFAVEVMEDAVEKGVKASIVITSGFREVGDEGAKLEEELISTAKKGGIRVLGPNTMGLISPDFNGTFAYANVQRGEIALVVQSGGIGAYMLDWAQRTRTGISFLVSMGNQADVKEYEVIDYLSRDPETRAIFVYLEGVADGEKFLDVVPDAASRKPVVFIKGGATAKGAEAVKTHTGSLAGSYEVFKAAIKTVGGIFVEDLSDFLNLTRFVTSSEPIKGDILVVTNSGGHGVLTSDAISRSGLNMVDIPDRIKDELRKILPVTSLPKNPLDLSGDAGRDRYAEAMKIVQDLDCTKLVIVQSLPVVSCSEVAKVLLNYKGKGVVGVMMGVDEDSALRILESASIPAFNFPEAAVRAIRYLTSKPNPRKKIRVAQPISSAVELTKGKEYLADYEALKLMEIYGIKTPKWGIVQSEEEAQRVADSIGYPVVMKISADKPLHKTEMKGVVMNVEKDMVKSVYKQLSQITKRVMIQEQLTGLEVFVGGLKDPVFGHTVLVGSGGIYVEVLKNVSYGISPVYEDEALEMLKESKVHDMLTARKRGYDEGSLIRTIVTVSRMIVDLNIKEMDINPLIVNENGAFAVDVRILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I4</accession>
    <name>F4B8I4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776851.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I4"/>
    <dbReference type="STRING" id="933801.Ahos_2065"/>
    <dbReference type="GeneID" id="10601565"/>
    <dbReference type="KEGG" id="aho:Ahos_2065"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_16_0_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="450"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="531"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="566"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="631" mass="67418" checksum="047AAD5402E6F015" modified="2011-06-28" version="1">MSSSKGEVGGAKDYGTTSDKLLRRSLNKWELLFLSVGGVIGSGWLFGALYTAAYAGGAGILSWVIAGILVMFVGLAYGELGAAIPKSGGIVRYPHYTHGGVAGYIITWAYFLSAASVPAIEATATITYLSALVPGLIYPNGVLTPLGILSAYIILILFFLLQYAGVGILGKVTHGVGWWKLIVPALTVVFLIAFYFHPANFTMGGGFFPSASMNASGFSGFAPVLYAIPTTGVIFSYLGFRQAVEYGGEGKNPQKDIPFAVIGALVIGLILYTLLQVAFTGAIDWSSVTVQVGNTTKPVTPGNWTALTQSNLVSGPYYTIMTTAKVFGPIASLFAIWSYILLIDAVLSPSGTGWIYIGTAGRTVYGFATNGYLPGLFLKIGRTKVPVIALIAATIIAAIFMLPFPSWESLVGFISSATVFTYIMGGIGLETLRKTAPDLKRNFKLPAAKVIAPIATLASGLIVYWSGFATLFYVVTAIFLGFPIFFGYYAVRLGVPKKIAYPLGIVDLAVILLTAYDFYTATSGLSAGNTIALILYTLIMVGVSLSNVAVLWNFSNDNVKKEIKASYWIFAFIFAIFLLSYFGALGLNPIIPFPTDVVIAAIVVLIFHYIAVKSGFRTEAIEEIIEETREP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K9</accession>
    <name>F4B8K9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8K9"/>
    <dbReference type="STRING" id="933801.Ahos_2090"/>
    <dbReference type="KEGG" id="aho:Ahos_2090"/>
    <dbReference type="eggNOG" id="arCOG01669">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169424_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="119" mass="12918" checksum="A4242146BF40292A" modified="2011-06-28" version="1">MFYLMAKIFVISTAGKDDINRATMAMNFALGARKNAGATVAFMFLGRGVETLLREAANAPQMKKLIEDMINAGIDVSYCGISLKNLGLNKDLIFDGIREVMGGVETAKRIDEGYAVVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M8</accession>
    <name>F4B8M8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776895.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M8"/>
    <dbReference type="STRING" id="933801.Ahos_2109"/>
    <dbReference type="GeneID" id="10601609"/>
    <dbReference type="KEGG" id="aho:Ahos_2109"/>
    <dbReference type="eggNOG" id="arCOG01147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083277_0_0_2"/>
    <dbReference type="OrthoDB" id="15074at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051158">
        <property type="entry name" value="Metallophosphoesterase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016538">
        <property type="entry name" value="UCP008292"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31302">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008292">
        <property type="entry name" value="UCP008292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="214" mass="24720" checksum="DACB26499D86BCAD" modified="2011-06-28" version="1">MLIAATSDIHSPRYLNDFFIALRYLPQHVDLVLLAGDLADKGKFMHFDPVYNALRKFQIVAVFGNEDFREEREKYKEKFPDVIWLDDEKKQLDDVTIIGSEGVIKKPTVWQKMKGIDEKFYTERKKKIEEMLCSSSSFKILLTHYAPTYKTVYGERPSAYPGLGDELIEELQCKPNLAIHGHAHYAKVTFAKLDNTKVYNVALPANKKFVIIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N2</accession>
    <name>F4B8N2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_2113</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776899.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N2"/>
    <dbReference type="STRING" id="933801.Ahos_2113"/>
    <dbReference type="GeneID" id="10601613"/>
    <dbReference type="KEGG" id="aho:Ahos_2113"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_1_2"/>
    <dbReference type="OrthoDB" id="101311at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="321" mass="34627" checksum="9ED684155B4FD929" modified="2011-06-28" version="1">MEILEYSIFLLGLISSFIVGGNNSATSLGILISTNALKRKYSYLLSALTIFVGVSLGSYSMQNSIYGTIYSRNLDMLESAVFSVLFASVIAFYYLNKLGIPSSLSQMLYPSMAVLVLISRGSLGFNWVKFWFTVTSWGFSPLLAIVSSITLYLAIRRLALKRKDLFSQMKIYKFLIVFSSIFTTFVTGANAIGIIVSAGLLSAPFYLVAPAYGIASALGIYLSSKKASIVVGFRVTRLGYISATSALVGSDIISEIFTLLGVPISITQTTMGGIIGLSFRSFGYDVKKQLSQVARGWGISPLVAIISSLAAFGVIRSVLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R4</accession>
    <name>F4B8R4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0898</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775700.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R4"/>
    <dbReference type="STRING" id="933801.Ahos_0898"/>
    <dbReference type="GeneID" id="10600370"/>
    <dbReference type="KEGG" id="aho:Ahos_0898"/>
    <dbReference type="eggNOG" id="arCOG05356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037618_0_1_2"/>
    <dbReference type="OrthoDB" id="39489at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="320" mass="37020" checksum="F714289AAF74FE3C" modified="2011-06-28" version="1">MRLNFITFESEILKDNPLQDPYIRRIGIIEPEDPIGKPIIIYLSGFLGSSISMLNYDPLSEDILSRVERLKKEGKINGSIIVLPDMFTKVGGNQYINSQAVGFYEDFLIKELIPYLEEKYKTRKIGLIGKSSGGYGALVLGMKYDEIKAIASHSGDAYFEYVYLPMFPKVIPYLRKFSSPEEWLSNFWKKQNKKKKEDLNVLNIIGMSAFYSPSSEGKILLPFDLDSGEIIEDIWRKWLEKDPVRLIEKLGENLKNKGVFIDVGIKDEFNIQYGARIIHNKMKKIGIEHYYEEYEDGHMNTSYRLDISISFIEKYLSQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B901</accession>
    <name>F4B901_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5751 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776939.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B901"/>
    <dbReference type="STRING" id="933801.Ahos_2153"/>
    <dbReference type="GeneID" id="10601656"/>
    <dbReference type="KEGG" id="aho:Ahos_2153"/>
    <dbReference type="eggNOG" id="arCOG05958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987615_0_0_2"/>
    <dbReference type="OrthoDB" id="38717at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043963">
        <property type="entry name" value="DUF5751"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19025">
        <property type="entry name" value="DUF5751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5751" evidence="1">
        <location>
            <begin position="11"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14893" checksum="C2C2196913B13DD8" modified="2011-06-28" version="1">MQPDYRPIIALIYVKDEELTETFRKMFKDVRLLGGKKIVANVISNSEYWNFFANAREAILDNLDLGLEIFTWKPNEVDKMIKKIQQYNYKGFITYCSDENKYNMRKILDILPTSMKANMLRDYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B968</accession>
    <name>F4B968_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR repeat-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775777.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase HTH" evidence="1">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="145" mass="17053" checksum="01ADDBC4BC8FC220" modified="2011-06-28" version="1">MQEEELKEKIKKMYEDGKTIREIAKELNMSYSKVRKILILEGVQFRGKLKQELVNKVIELAKQGYSANKISKEMRLNSNTVLRILRKNNLVKAKRKLSKEDIEKIKLMYESGSSIYKIAKELKISTNLVVYHLKKLNIYKPQTYS</sequence>
</entry>
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        <submittedName>
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        </submittedName>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D0"/>
    <dbReference type="STRING" id="933801.Ahos_1037"/>
    <dbReference type="KEGG" id="aho:Ahos_1037"/>
    <dbReference type="eggNOG" id="arCOG03182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_2"/>
    <dbReference type="OrthoDB" id="10712at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ArsR-like_HTH"/>
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        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039422">
        <property type="entry name" value="MarR/SlyA-like"/>
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    <dbReference type="InterPro" id="IPR023187">
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33164:SF43">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REPRESSOR YETL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33164">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH marR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="16132" checksum="D96770ABB3D8836B" modified="2011-06-28" version="1">MEDWEIILKGNRKLRELLQKEAEKRGLSYTEVSTLYFLKGGEKNVSSLAEFVGVNKSTMVEILDTLENEGLITRSRDEKDRRVVLVKITQKGLEELENVRQGYKNLIKDILSRSEGNIIKFFQIVIEEVSVKEKASNVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E3</accession>
    <name>F4B9E3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2218</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
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    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9E3"/>
    <dbReference type="STRING" id="933801.Ahos_2218"/>
    <dbReference type="KEGG" id="aho:Ahos_2218"/>
    <dbReference type="eggNOG" id="arCOG01833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG08402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1067930_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041800">
        <property type="entry name" value="Hsp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="165"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="170"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="29317" checksum="637C6D14F2C56875" modified="2011-06-28" version="1">MGSDKSVDGRVLVFPSTSSKDLRVTAYDPLRGVTMAELEVRISYATGKHINLRALSVKYKHFQISFTQLKSQIYLVSFKSSRGEYILGSKMPSKKKGDIFDYFDEMIKQMEEEFEELEKEFFKAAGKGEVKTFGPYVYGFSMTIGPDGKPIIEEFGNVKRLGSKPILSEEREPLVDVIEKGDEVRVVAEVPGVDKNNIKVRLNGKTLILSAQEGDKKYYKEIELPTEVDENSAKATYKNGVLEIVFKKAKVESGKEIKIE</sequence>
</entry>
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    <accession>F4B9F1</accession>
    <name>F4B9F1_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">TRASH domain protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2226</name>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777012.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F1"/>
    <dbReference type="STRING" id="933801.Ahos_2226"/>
    <dbReference type="GeneID" id="10601731"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRASH" evidence="1">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="46" mass="5454" checksum="033C8F23EF25D498" modified="2011-06-28" version="1">MKDPVCGEDVKNTSYKYVYKGITYYFCSPMCMAEFKKNPEKFVKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N2</accession>
    <name>F4B9N2_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    <dbReference type="GeneID" id="10600538"/>
    <dbReference type="KEGG" id="aho:Ahos_1064"/>
    <dbReference type="eggNOG" id="arCOG00985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_1_1_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21154">
        <property type="entry name" value="PUA_MJ1432-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.120">
        <property type="entry name" value="Pre-PUA domain, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHP03684"/>
    </dbReference>
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        <property type="entry name" value="DUF1947"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
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        <property type="entry name" value="PUA"/>
    </dbReference>
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        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
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    </dbReference>
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        <property type="entry name" value="MCT-1 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="70"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17328" checksum="9B146C2D071327FF" modified="2011-06-28" version="1">MQRHFLSEKETEELLEKIKEKYNISLSPQKVEIGKEKKEVYYFLDGVLSFFSQDLIPTLCAIYKFNISLPSVKVDEGAVRAVLKGADLFVPGIKEYNCNCKPGDIIAVTTMEGKAIAVMKVLISKEDAEKEKRGKFSKNLHYLGDEIWKMCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9P8</accession>
    <name>F4B9P8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma-glutamyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9P8"/>
    <dbReference type="STRING" id="933801.Ahos_1080"/>
    <dbReference type="GeneID" id="10600554"/>
    <dbReference type="KEGG" id="aho:Ahos_1080"/>
    <dbReference type="eggNOG" id="arCOG04053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_3_2_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052896">
        <property type="entry name" value="GGT-like_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43881:SF1">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSPEPTIDASE (AFU_ORTHOLOGUE AFUA_4G13580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="476" mass="53209" checksum="9837774709055A96" modified="2011-06-28" version="1">MISAVGKKVVATQNYIASYVGAKILESGGNAFDASIAISAVLSVVLPHTSGLGGDGFILIKSSEGIKAYNSSGWSPKNLKAEKINERDPNSVVVPGLVDLWRYLQENYTTMDLYEPLKPAISLATNGFYIGRALHHAIITSPKFNPQFEKLFGNKRFGDFIKLPRLGKILKEISKDPREFYEGKISQEIVEGLKTQGVPVEIEDFSQFHGEEVSLLKIRYKDYDVYEFPPNTQGLTTLQILKMVELSRINKLPYNDIKRVNNHVKISILAYDDRDRYIADPKFYQPPSYLLDEGYLIQKMTTEGIPVNLYDGDTTFFVVSDGENEVGFIQSLFYPFGSGIIVDDIVFNNRGYGFKDGVNKPEGRKRPLHTLSILLANKDNEDLIIGCAGGDLRPQIHSEVFEYYADYSMEIDEAVNAPRFMYVNGKVIAEKRLGIPATQTEYLSSEVGIVQAMKRKGNEFIAVADPRSEGVALPVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S3</accession>
    <name>F4B9S3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lysine biosynthesis enzyme LysX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1105</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RimK family. LysX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S3"/>
    <dbReference type="STRING" id="933801.Ahos_1105"/>
    <dbReference type="KEGG" id="aho:Ahos_1105"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009085">
        <property type="term" value="P:lysine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000025">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000058">
        <property type="entry name" value="Alpha-aminoadipate--LysW ligase LysX protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054562">
        <property type="entry name" value="LysX/ArgX_preATP_grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011870">
        <property type="entry name" value="LysX_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02144">
        <property type="entry name" value="LysX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22626">
        <property type="entry name" value="LysX_preATP_grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="76"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="29488" checksum="51EE82218468C860" modified="2011-06-28" version="1">MIVKSLNDKGIQYDIINVAQEPLPFNRALDRYNVAIIRAISMYRSLYSAAVFESVGVHTINSSEVITVAGDKILTYSKLFKAGVPIPDSVIAMSPDATLKAYEQIGFPLIDKPPIGSWGRMVSLIRDIFEGKTIIEHREMLGNSALKVHIVQEYIKEKNRDIRCIVMGKELLGCYARNIPPNEWRANVALGGTPSPITIDEKLRETALKAASVVNGEFISIDILEHASKGYVINELNDTPEFKGFMLATGINVAEKLVDYIISNFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U3</accession>
    <name>F4B9U3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="19">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="19">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="19">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="19">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="17" type="primary">nnrD</name>
        <name evidence="18" type="synonym">nnrE</name>
        <name evidence="22" type="ordered locus">Ahos_2288</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18 19">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18 19">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17 19">
            <text>(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 19">
            <text>(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="18 19">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="19"/>
    <dbReference type="EC" id="5.1.99.6" evidence="19"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777074.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U3"/>
    <dbReference type="STRING" id="933801.Ahos_2288"/>
    <dbReference type="GeneID" id="10601795"/>
    <dbReference type="KEGG" id="aho:Ahos_2288"/>
    <dbReference type="eggNOG" id="arCOG00018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="OrthoDB" id="15148at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0413">Isomerase</keyword>
    <keyword evidence="22" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 17 19" id="KW-0520">NAD</keyword>
    <keyword evidence="8 17 19" id="KW-0521">NADP</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0630">Potassium</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="21">
        <location>
            <begin position="7"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="20">
        <location>
            <begin position="211"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="127"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AEE95159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="500" mass="53487" checksum="2466A02F967807F2" modified="2011-06-28" version="1">MITTKRMRALEINSEALGVPTLLLMENAGRSVKDEIIKKLGNVKGKSVYVFVGHGGKGGDGLVAARHLASEGAKVTVILLGENKHKDALLNLAAVQEMDYSINIITIKDPEELKPVEGDILIDALLGTGFSGKPREPFRTAIKVFNQSKGFKVSIDVPSGVDSDTGETPGDYVVPDLVVTFHDMKTGLSKFNFETKVENIGIPPEAEIYVGPGDLMVNIKKREMKSRKGAGGRVLIIGGSKTFSGAPALAGLAALRTGADLVYIASPGQTAYTIASYSPDLIAIKLNGENINPGNLDELKPWIEKSNAIVIGPGMGLAEETIEASKEIVTYLKEINKPAVIDADALKSIKGFELYPFAVITPHAGEFKIFFGYDVKDNPRERISQVINSAKRANCVVLLKGYFDIISDGERFRLNKTGNPGMTVGGTGDTLTGIVATLMAQGLSPFDAASIGAFINGLAGSLAYAKYGNHITPSDLVNEIPEVINNPLEAFKKKPYIRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V4</accession>
    <name>F4B9V4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferric uptake regulator, Fur family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777085.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V4"/>
    <dbReference type="STRING" id="933801.Ahos_2299"/>
    <dbReference type="GeneID" id="10601807"/>
    <dbReference type="KEGG" id="aho:Ahos_2299"/>
    <dbReference type="eggNOG" id="arCOG01868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_4_2"/>
    <dbReference type="OrthoDB" id="21318at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900376">
        <property type="term" value="P:regulation of secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202:SF7">
        <property type="entry name" value="FERRIC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="ZINC UPTAKE REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="134" mass="15068" checksum="F8D635C3433AB453" modified="2011-06-28" version="1">MEIDPVEILRKKGLKVTPQRLAVLKLLSKGGHYSGEQIFEELKKNEPSISLSTVYNALEALESAGIINSFEANGITWYEMRRNPHVNVICEDKNEIIDVDINIDEIVQQLKNKGINVINLSVVAYADCSKIENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V5</accession>
    <name>F4B9V5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine/uridine-preferring nucleoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2300</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777086.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V5"/>
    <dbReference type="STRING" id="933801.Ahos_2300"/>
    <dbReference type="GeneID" id="10601808"/>
    <dbReference type="KEGG" id="aho:Ahos_2300"/>
    <dbReference type="eggNOG" id="arCOG04558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_2_2_2"/>
    <dbReference type="OrthoDB" id="33780at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02649">
        <property type="entry name" value="nuc_hydro_CeIAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015910">
        <property type="entry name" value="I/U_nuclsd_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01247">
        <property type="entry name" value="IUNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="34415" checksum="840E14E9CC105F6B" modified="2011-06-28" version="1">MPRYAIIDSDTASDDTIAILLASKFFKLLGITIVAGNVKFENEIKNALFSVEYFNLGVPVFIGSSRPIMGKWRTVEEVHGNNGIGDWKIQEPKISPEREHAVDAIIRLSKEYEGELEILAVSPLTNLALAYLKDPTIVKRIKKVWIMGGAFSRGNTTPIAEFNFWVDPEAAEIVLNAGFDITIVPWETTEDAAEITDDEWKKIESINSKASTFFINVNRTLREYSKKYEGRGSIHPDSLTVTIAYDNSLILQQGEFKVDVETCSISRGAMLVDWYNFSKSKNAKIVLKADKQKFINYLFSILEQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V9</accession>
    <name>F4B9V9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777090.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V9"/>
    <dbReference type="STRING" id="933801.Ahos_2304"/>
    <dbReference type="GeneID" id="10601812"/>
    <dbReference type="KEGG" id="aho:Ahos_2304"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="OrthoDB" id="50367at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00838">
        <property type="entry name" value="MPP_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="323" mass="36548" checksum="A5F5CBB148ED8072" modified="2011-06-28" version="1">MGLFNRKKNNNDSKQVRILFTSDLHGSDIVFKKFLNAGKMYKVDALIIGGDLAGKALIPIIELNDGNYEIDGNIFSKDLLAQKISEIRNQGNYYVIVSREEFEDMKHDKRKVDEAFKNAMTEVVRNWIKIAEERYKGLNIPIYVNLGNDDPEYLFNVLDESDIMIKTEGKVVNIDKYEMISYGYVNPTPWHTPREMPEEKLYEALKHEVEKLSNPSESIFNFHAPPYGSNLDNAPLLDENLKPVVKGGEIVFTHVGSQAVRKIIEETQPLLGLHGHIHESRGFDKIGRTIVINPGSEYGEGLLHAALVVLEGGKVKAYQFIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA32</accession>
    <name>F4BA32_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="8">PRA-PH</shortName>
            <ecNumber evidence="8">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisE</name>
        <name evidence="9" type="ordered locus">Ahos_1145</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775945.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA32"/>
    <dbReference type="STRING" id="933801.Ahos_1145"/>
    <dbReference type="GeneID" id="10600619"/>
    <dbReference type="GeneID" id="42778193"/>
    <dbReference type="KEGG" id="aho:Ahos_1145"/>
    <dbReference type="eggNOG" id="arCOG02677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123337_1_1_2"/>
    <dbReference type="OrthoDB" id="39686at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="10812" checksum="B594352FF3D8ACDE" modified="2011-06-28" version="1">MSEVLDKLYEIILQRLEKMPENSYTAELVKKGKGYIARKVGEEAVETIVASLYEGRDRFISEAADLMYHLWVLMAVEGVKPEELYAELKRRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA76</accession>
    <name>F4BA76_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NADH-ubiquinone oxidoreductase subunit 5 (Chain L)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775989.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA76"/>
    <dbReference type="STRING" id="933801.Ahos_1190"/>
    <dbReference type="GeneID" id="10600666"/>
    <dbReference type="KEGG" id="aho:Ahos_1190"/>
    <dbReference type="eggNOG" id="arCOG01539">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_277666_0_0_2"/>
    <dbReference type="OrthoDB" id="19089at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="600"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="764"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="798"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="832"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="908"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="943"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="986"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1022"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1055"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1124"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="118"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-Ubiquinone oxidoreductase (complex I) chain 5 N-terminal" evidence="8">
        <location>
            <begin position="561"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="657"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1145" mass="126416" checksum="02F051709F21DC22" modified="2011-06-28" version="1">MISIFILIISLALSSIVFFIKEKKSAFILSIIAIGINSFFLLRRGLYENFFVGTDIGYFGLTVNDLNYSFLVVILAVTIVTTVYSLRYMEEKIGEGSWGLYYGLYSLFAVSMLYISISTNLLEIYVFLEISLVTSFLLILLYGYGDRRRISLMYFIWTHIGTILLLASIIIIGLEFHTMDVYSSYGVTNVLSSIPFAELVLLLGVIGMLVKSAQAGFNIWLPYAHGEAPTPISVLLSPNLVGLGVYVIILYFYLFPAFSYLAPIFIGWAVITMIYGGINALAQRDFKRFLAYSTVSQMGYMLLGSSAAYMLGLGQNVNQLPIGILSAVLIYASHGFGKAILFMSAGASITELNTRNIEELGGLYLSSPLHTTLSFIGLLNILGLPPTVGFLSEVLLLLAVGQLIHVSSPLLFILLVLGVTIGVGISSGYSAYLFKKVYAGRKEIKSIDKIAEYSIPMLLLALISGVFFFYPSLLSISFNNFIQTLPPSTHACLFLIVILPALGSLISLISGKLNQDVKGAIISILIGLSMILSIYNLISTVSTPHPNFFVPAYTFTAFGYFQFSSSLLQAILAVFVSTLSFFIAIYSIGYMKEDNVLRRYWGFFGFFISSMLSVVLGDNILVFLAGWEGTSLASYGLISYWLDDNEKNVVGDFKRFVLGIEYLSRPTTSGIRALVFTRIADIGTIMILGYLIFLTSQSTLSGITVIYPVLHSSIQGLFSELPSLLILPYSVIFLILLYLGGLSKSAQFPFTQWLVTAMTGPTPVSALIHAATMVNLGAIFTFLTYPFLIQGYSASLEIFLEIIVGINIFTAIFTSFNALVSNEQKVILANSTADQISLMILSSSLGGLLAILLHNFALVYAGISIGLIQMIAHGIYKATLFMNAGSIIHYIESRYIGVYPNLYKKLKLLFVLQLFAALNLANIPLFIGFWAHSFISYLTSYNSYIDIIYIGLEFAGAIYIIRYIARLFMYGSGEKEAEGHISSIMYVSPAILMISSIILAIEIFPLVSFIGKGFSVPPNVFIYNNLDFIVSLVGIAIAFMIYSGEMFNNKFLSPIINFFYYGWYIYPIMDIAGILSLKVSSGIFNYVETRGIDNSLNYKLPNGIIYYGGRLFNKLQNGLLRDYLGYYVGGIILLMVIVIILLLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L8</accession>
    <name>F4B3L8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774824.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L8"/>
    <dbReference type="STRING" id="933801.Ahos_0012"/>
    <dbReference type="GeneID" id="10599452"/>
    <dbReference type="KEGG" id="aho:Ahos_0012"/>
    <dbReference type="eggNOG" id="arCOG07323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985584_0_0_2"/>
    <dbReference type="OrthoDB" id="34339at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31464" checksum="3EA0DECC0E6807C7" modified="2011-06-28" version="1">MIVRIFKDDETYVINNFNGNIYGSNAYEAGRVFYFIQKAIYSMPRLYGKVNDFKDSLNSWKKAFDETLSSMISLMLTEGRIKFYANFEIESEILNIKGKIDGNKVSLSSSLLKIPEGITNDLKGVILLDSFYFNHMERKRPFILPSARDGFLASFYKFVVFQSEGISGIPKSMGIAAEFINSISINPGYKDEILGHQIIVNDEGLFIDDQPAYNSFSEMLSLFALKYFLLNTTSNDVLIIEDIEAHLSDKGKALLNEYLKTAKCNIVFVSNYSKFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M6</accession>
    <name>F4B3M6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774832.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M6"/>
    <dbReference type="STRING" id="933801.Ahos_0020"/>
    <dbReference type="GeneID" id="10599460"/>
    <dbReference type="KEGG" id="aho:Ahos_0020"/>
    <dbReference type="eggNOG" id="arCOG01262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_2"/>
    <dbReference type="OrthoDB" id="24596at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004453">
        <property type="entry name" value="PRK05786.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF1">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE92915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="239" mass="26420" checksum="06DE9B18D91F3DBF" modified="2011-06-28" version="1">MRLKDKAILIVGVSKGLGYALAYFLLKEGAKVIINSGNKEKLQEIVDSLKNLGEINYISERVVDLSSAKDVLSKSYELIKRKLDGIVISIGGYIEDNVENLTGLDEMINNHVKYPLYITSAALNYLNEGSTIVLISAMRGIDKALPNQLSYSISKSATAKAVEVLASELLDKQIRVVGIAPSWIYGDFQPNRDWKKLRKLGDVKAPPEDFARVITWLLTDEAEWINGVVIPVDGGARLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P6</accession>
    <name>F4B3P6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775999.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P6"/>
    <dbReference type="STRING" id="933801.Ahos_1200"/>
    <dbReference type="GeneID" id="10600676"/>
    <dbReference type="KEGG" id="aho:Ahos_1200"/>
    <dbReference type="eggNOG" id="arCOG06038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712935_0_0_2"/>
    <dbReference type="OrthoDB" id="36828at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="44267" checksum="73D2D9EB18B31F86" modified="2011-06-28" version="1">MISEKLISDLRFYDKPLGVFFTADNCKECDELIKKIKELPISKRLMIEEVNGLEYPEYLNRLTRGMIPTLSILSPDLGIMGVIESTDVKEIEAKLREIVEAYYKGYKGERLSEFIPEPLEVGKEIIYDVMDALLASYPADFRMIEFYKFISSINKDYSKAEKVIKPLNEISEFLLGRKKTINANSIYTTEISFYTIYGLRKVEDLLQLIGDDGIVYRSTRKQNKGLLIDEVMAGNALLTQYENTLNDTYLNYAKKIYDFIINNLSHEKGFRDTLIKDELSKITFLEPLANSEAGIFFARYWAITGEEKSADFAKKAINCAFAGSSDPRVLARISIALIKLQDLIRTNEKGPYSDLRIEFSKQAQCKYLKDGKCYEKIEEIKISYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q3</accession>
    <name>F4B3Q3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="14">XPD repair helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_1207</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q3"/>
    <dbReference type="STRING" id="933801.Ahos_1207"/>
    <dbReference type="KEGG" id="aho:Ahos_1207"/>
    <dbReference type="eggNOG" id="arCOG00770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006515_9_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:DNA helicase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
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        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="521" mass="59596" checksum="34F3310B50801760" modified="2011-06-28" version="1">MKRGFLVALNSPTGSGKTLFSLVVGTELKGKVAYIVRTHNEYYPVYRESKRLEKSFSFLVSKALACPFSTADVNPEDIKCSSCDIFSSIPIKVDDYPFSFLSKLKKEGEEEGFCPYYSLLDSLSNSDVIVLTYPYFFIPRLREALGLDFSQYVVVVDEAHNLDRLNELEERKLNITIIDRAISQISNTTVIEILRRLKEEVKKKALSEEKYILVQDYPKLTDNELEIIKEEYEALREKMIKEKRIKQIYLNNIIKFYETEGKVFSYKGSLVKKPITPEKYISILNDPSLSVILMSGTLQSKEFLRNVLGITRQIEYIDTEKEMKKKLSGTIDCILALDVTSAYSLRTKEMWKKYASYILKIFYQAKGYVLAVFPSYSLMNEVMKLVNLPKLVEDEKTSLEDVYKLKEKSIIAAVARGKLSEGIELTKDGKSLISDVVLVGIPYPAVDDYLKLQAEEISKITGQDVKDLLINTTALIAVKQAIGRAIRSVNDRANVWLLDKRYDSIMWKTKINCLNPKKIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W1</accession>
    <name>F4B3W1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1265</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776064.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W1"/>
    <dbReference type="STRING" id="933801.Ahos_1265"/>
    <dbReference type="GeneID" id="10600746"/>
    <dbReference type="KEGG" id="aho:Ahos_1265"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_859484_0_0_2"/>
    <dbReference type="OrthoDB" id="5620at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21109">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF34">
        <property type="entry name" value="TUNGSTEN-CONTAINING ALDEHYDE FERREDOXIN OXIDOREDUCTASE COFACTOR MODIFYING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="20"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="322" mass="37476" checksum="7526DA172EDA5B89" modified="2011-06-28" version="1">MNRDIKAIRWFIKTQLLKDPFNPGYATFKVTSRCNLHCTFCNPEYYNGELGEGSTETIKKIIDNLRDSSIVVLSFEGGEPTIRKDILELLEYAHDGSFYVMLTTNGYRLNDDEFLTKLADRIDFLHYSIDEYHWNVKALDTLCRFRQYGIKVNVQTVVTRYNLNKLEDKIKKVRECNYKILVLPAVDYPDAKVKLAPDPEQLYNVLSELKKKYSSTLNNSWGFIKALKGEYPKRLVSYAITIYPNGDLPYPDDINGEIVGNLSKEKLNDILKSPRVKELQRKMLENQAKFEYLHLQTASFNSLRDLAEYVKDMVKWRFTGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z5</accession>
    <name>F4B3Z5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell wall biosynthesis glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774876.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z5"/>
    <dbReference type="STRING" id="933801.Ahos_0064"/>
    <dbReference type="GeneID" id="10599505"/>
    <dbReference type="KEGG" id="aho:Ahos_0064"/>
    <dbReference type="eggNOG" id="arCOG01397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055387_0_0_2"/>
    <dbReference type="OrthoDB" id="42128at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="305" mass="35624" checksum="EB213991140A0E4A" modified="2011-06-28" version="1">MISVVITAYNRKEFLIDAVKSVKNANEIIVVKNFKDDKIDKLLVNEYKVKEIFCDNDNAGYFMAEGILHSSGDIICFLDDDDMFTENKLNVISKAFSDNDIIFSYNSRFIINEKGDKIGEEVLRDKIVDSKNIKYLFKNRIYFNSSSMCIRRRVLDKNLENLYKIRRLVDNFFLLSAISEKGKIRIISDKLTYYRLHSSSSRHITRNFEDFITYRAKYFDDAIHDNAIMIEAFKGKAKYAAKCSKKMAEIQKCLLEGKRCGKLVLCSIYTLKTLVYVNLASFMSLFLPNLPRKMEFNRYINELKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B459</accession>
    <name>F4B459_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="2">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1284</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776083.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B459"/>
    <dbReference type="STRING" id="933801.Ahos_1284"/>
    <dbReference type="GeneID" id="10600765"/>
    <dbReference type="KEGG" id="aho:Ahos_1284"/>
    <dbReference type="eggNOG" id="arCOG01481">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043773_0_0_2"/>
    <dbReference type="OrthoDB" id="371831at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01571">
        <property type="entry name" value="NAPRTase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053190">
        <property type="entry name" value="NAPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035809">
        <property type="entry name" value="NAPRTase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006415">
        <property type="entry name" value="PRK08662.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43202">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="9">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="8">
        <location>
            <begin position="115"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase C-terminal" evidence="10">
        <location>
            <begin position="349"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="386" mass="43245" checksum="6A033F6640230A51" modified="2011-06-28" version="1">MKFYTATEDEILGGKITDIYFDRTIKTLEHLGINNVEVRMEFHSYGLPKGYSWAVFTGLEEALKLLEGKPVTVYAMPEGTLFKEIEPVMIIEGNYLDFGVYETALLGILRHESSIATKAARIKKLAQDKTVLFFGLRALHPAIAPMADRAAYIGGSDGISGAFDKDIFDIEPSGTMPHALMLSIGDNEKAWKAFDEAMDEKVPRIALVDTFEDERTEALKAAKLLGKRLAGVRLDTPSSRRGNFRKIVQEVRWTLNINGFSHVKIFVSGGIDEEDVVELRDYVDGFGVGTSISMPPSVDLSADIVEKKIDGKWIPYTKRGKWPGAKQVYRCSGFNDIITLLEDKPIEGCKPLLVKYMENGKIIRDLPSLKEIRNYVLEQLREVQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B469</accession>
    <name>F4B469_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spt5</name>
        <name evidence="8" type="ordered locus">Ahos_1294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776093.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B469"/>
    <dbReference type="STRING" id="933801.Ahos_1294"/>
    <dbReference type="GeneID" id="10600775"/>
    <dbReference type="KEGG" id="aho:Ahos_1294"/>
    <dbReference type="eggNOG" id="arCOG01920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113589_0_0_2"/>
    <dbReference type="OrthoDB" id="371863at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09887">
        <property type="entry name" value="NGN_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="N-utilization substance G protein NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="16409" checksum="3B28DBA0844A543D" modified="2011-06-28" version="1">MESKIRNYYAVKVTGGQEVSVGLMLEERAKTNNIPEIYSIIVPPGLKGYVIVEASGPHVVKLLIAGIRHVRGIAQGLVPKDDIIKMVSKKVTGPTIKEGDLVEVVSGPFRGMQAQVVKVTEGKGEVVLNILESAFPLQVTIPVDQVKPVKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B494</accession>
    <name>F4B494_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeS assembly protein SufD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776117.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B494"/>
    <dbReference type="STRING" id="933801.Ahos_1319"/>
    <dbReference type="GeneID" id="10600805"/>
    <dbReference type="KEGG" id="aho:Ahos_1319"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_736928_0_0_2"/>
    <dbReference type="OrthoDB" id="300624at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="144"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="382" mass="42178" checksum="2139E5C3DAD8B495" modified="2011-06-28" version="1">MPLVDIDSAKSFLSSLENRSEREELFSIYERLPYQLINDSPTVKHYTRWENLDKLNLKIESLDGKRGGNYPSIPGYVRVVIHNNLVKETPLEFSSSVGNSLIKPEDHKLVSLTLSLSRKLEIKQGGKYLICHYTDVEGNFSPINIEIKVPEGESADVIYYSENKGKSMNSAVISASVSKNSSLNLTLVNKGNLSYNFVYSKARVEGSINTYIISSGFSEGHVEYHSYLEEGAQAFFSSRALGVENNNIDVITNVYHDGPKSVSNGFMKSVSAGSSFVVTRGDARINEQAIDSSTSIIGKAIMLGESTAVVAPMLEVRTGRVITAKHSASVSKVPEDLIFYLQNRGFDKKSAEGLIIRGFMEEEGDTEIVKKLIDEIITSLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B2</accession>
    <name>F4B4B2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit Rpo6</fullName>
            <ecNumber evidence="3">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpo6</name>
        <name evidence="3" type="synonym">rpoK</name>
        <name evidence="4" type="ordered locus">Ahos_1337</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776135.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4B2"/>
    <dbReference type="STRING" id="933801.Ahos_1337"/>
    <dbReference type="GeneID" id="10600823"/>
    <dbReference type="KEGG" id="aho:Ahos_1337"/>
    <dbReference type="eggNOG" id="arCOG01268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112527_4_0_2"/>
    <dbReference type="OrthoDB" id="10567at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002207">
        <property type="entry name" value="PRK01099.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002209">
        <property type="entry name" value="PRK01099.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="10647" checksum="B3FEC3966571CE1B" modified="2011-06-28" version="1">MSELEIVNKLNVAYIESWKKRLTIYEIARIVSARALQLAMGAVPLIDISGFKPEQLNSISIAEEELKRGVLPITIRRRFPNGKVELISVKEILGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E3</accession>
    <name>F4B4E3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774949.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4E3"/>
    <dbReference type="STRING" id="933801.Ahos_0139"/>
    <dbReference type="GeneID" id="10599579"/>
    <dbReference type="KEGG" id="aho:Ahos_0139"/>
    <dbReference type="eggNOG" id="arCOG10433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923196_0_0_2"/>
    <dbReference type="OrthoDB" id="38571at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="339" mass="40262" checksum="FDC9EE24B261D9CE" modified="2011-06-28" version="1">MQCSDLLKLVVEGKIDKEIGAYYDCFLSLQHFLRFNVALKLKRKVIKIGNYVYFDLDYDRPSSFISGIDDTTGKIFTMPVRMCGIYYETEEEIRKCMGFDYHYYEKFEYATNVKIRIQGDLVMDVIRAYDKKEELFKYINENKENFRQLWESFVRAELGKNKEMQNAEVLIGTYQELMDFALNTRVYKEEDRKDVIKVVKLLRMLENDILTIAKKYGIEVHNLYEKPRSSEPERYKCIRFLDIQEFARKLREKKAEELSENFDNFVLNQENTVKIRIGHYTTPHEISLTGVITDVVEGRRVNALILSPQKITVKHPEHGVNEFYVPKPSYVQFRLMEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I5</accession>
    <name>F4B4I5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I5"/>
    <dbReference type="KEGG" id="aho:Ahos_0182"/>
    <dbReference type="eggNOG" id="arCOG05362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2270887_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="102" mass="11096" checksum="890ABE329B267D7B" modified="2011-06-28" version="1">MTMLMSDQPVGLGGKSQMVPTQYPINLQAVAQQVVMFLRAQGFRAWPMVGQNLAVIQAQHHSLLGTLTGQNKAYTIRLCQGQGFIMVETGIANLMQDCNAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M0</accession>
    <name>F4B4M0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="3">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1367</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carbamoyl phosphate + L-ornithine = L-citrulline + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the aspartate/ornithine carbamoyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776165.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M0"/>
    <dbReference type="STRING" id="933801.Ahos_1367"/>
    <dbReference type="GeneID" id="10600854"/>
    <dbReference type="KEGG" id="aho:Ahos_1367"/>
    <dbReference type="eggNOG" id="arCOG00912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="OrthoDB" id="4696at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000008">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="6">
        <location>
            <begin position="6"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="5">
        <location>
            <begin position="152"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="NCBIfam" id="TIGR00658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="307" mass="34508" checksum="44681D3F3C032225" modified="2011-06-28" version="1">MLKGNSLLCLLDFERQDIERIMEVSFLMKNFVKTNSVPKSLDGKRVALIFEKPSTRTRVSMESAIYLLGGYPIVLNKSDIQLSRGEPVEDTARVLGRFVHGIGARVLSHESLIKLRDYSGLPVINLLSNLSHPLQALADFMTIKEKFGTYEKPIAFVGDGGDNVLVSLMALAAKMGLELRVASPKELRPKPEIWKRIEEESENSGAVIEFYDDPYFAVRGSFVVYTDVWVSMGEEDIANKKKELLKNYRVTTDLMRHASKDAIFMHCLPAMRGEEVDAEVIDGKQSAVWDEAENRLYTAMSVLSLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M6</accession>
    <name>F4B4M6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776171.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M6"/>
    <dbReference type="GeneID" id="10600860"/>
    <dbReference type="KEGG" id="aho:Ahos_1373"/>
    <dbReference type="eggNOG" id="arCOG05984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127132_0_0_2"/>
    <dbReference type="OrthoDB" id="34451at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="249" mass="29137" checksum="C378468CA2435AC2" modified="2011-06-28" version="1">MICTKCGKNQAEVLISGKYLCVHCARSEIVKRFRKELSLSKFLDREDRLVILTTENFMDVSSLIEKLIYNICKSCKLDVLIDKINEEEDINKTLWKIMIKIRETDFKKIILPFTADFFLSYLIYSISTGYMGYLELYSLVVEVYSKKIFVPFYSTPRFELLGFSEITGELKTKDAFLNEIIKWASKQFADNEIYHTFGSSINVIKLENRKRCKICNAIIERGDCCKYCLESFSHLCSEQSQKQELEERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q5</accession>
    <name>F4B4Q5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Riboflavin kinase</fullName>
            <shortName evidence="17">RFK</shortName>
            <ecNumber evidence="4 17">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 17">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 17">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 17">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ribK</name>
        <name evidence="19" type="ordered locus">Ahos_1402</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>riboflavin + CTP = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="4 17"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776200.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q5"/>
    <dbReference type="STRING" id="933801.Ahos_1402"/>
    <dbReference type="GeneID" id="10600891"/>
    <dbReference type="KEGG" id="aho:Ahos_1402"/>
    <dbReference type="eggNOG" id="arCOG01904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088476_0_0_2"/>
    <dbReference type="OrthoDB" id="30955at2157"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706:SF1">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 17" id="KW-0288">FMN</keyword>
    <keyword evidence="11 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="18">
        <location>
            <begin position="94"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="97"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEE94285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="24867" checksum="3F624992E3696060" modified="2011-06-28" version="1">MLTEICTLSRIINLVKEKGEITQQILAKEFNISQQSVSRKLKELEDANLIIRIETKDGEIIRLTEKGENLLLNCLNEISSAIAFQHEIRIKGKVTSGLGEGKLFLSLPYYENSFKKFLGFTPYPGTLNIVIYDRISFENRLLLDTSRYINIPEYRDENRVLGAVKAYPARINDLEPAAIVIPLRTTHPKSVIEIISPYYLREKLSLKDGDEVLIEALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R0</accession>
    <name>F4B4R0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776205.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R0"/>
    <dbReference type="STRING" id="933801.Ahos_1407"/>
    <dbReference type="GeneID" id="10600896"/>
    <dbReference type="KEGG" id="aho:Ahos_1407"/>
    <dbReference type="eggNOG" id="arCOG07217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010189_0_0_2"/>
    <dbReference type="OrthoDB" id="42865at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="13994" checksum="6326EE4E3BFD4371" modified="2011-06-28" version="1">MKIKDIIDLPLPGLKESLEYGISEAEYLGKEFIGITLLNGDGIILHVNPYSDEIIEAYLMTIKQNSEKRLIGVFKYEGGNTYFIYEITDLVDYINRLTNERVVYVEVIKDVLEDFLLNAVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S3</accession>
    <name>F4B4S3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="12">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">top6A</name>
        <name evidence="17" type="ordered locus">Ahos_1420</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 13">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4S3"/>
    <dbReference type="STRING" id="933801.Ahos_1420"/>
    <dbReference type="KEGG" id="aho:Ahos_1420"/>
    <dbReference type="eggNOG" id="arCOG04143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037229_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000655">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000011">
        <property type="entry name" value="Type 2 DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049333">
        <property type="entry name" value="Topo_VI_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003336">
        <property type="entry name" value="PRK04342.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20768">
        <property type="entry name" value="Topo_VI_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="10 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="8 12 13" id="KW-0799">Topoisomerase</keyword>
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        <location>
            <begin position="76"/>
            <end position="152"/>
        </location>
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        <location>
            <begin position="156"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="16">
        <location>
            <begin position="201"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="12 13">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20768"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE94303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="386" mass="44911" checksum="4741118822E50BFA" modified="2011-06-28" version="1">MSEFISKVDIEARKRAAEILKKKFLTLLDQINKGEPLVLEIPKRTLNNTVYDENRKLLLLGEEKLKRSFLDLNEARRFMQTTLMASIIYDALVNNEYPTIRDLYYRGKHSIVLKAPNGKTYEENTWDEQKESDSVIVDIEVFTSLLREDMLILSKEKGKVVGDMRIRSGNDVIDLSKMGHGAYSIEPTPDLIDFVDVKADFVLVVEKDAVFQQLHREGFWKKYNAILVTSAGQPDRATRRFVRRLNEELKLPVYIMTDADPYGWYIYSVFRIGSINLSYESERLATPNARFLGVSMGDIFGTPKKKAYLTERERNAFIIKAKDYDIKRALEIKNYEWFKTKAWQEEINMFLQKKAKLEIEAMASKGLKFLAFQYIPEKIQTNDFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T7</accession>
    <name>F4B4T7_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T7"/>
    <dbReference type="STRING" id="933801.Ahos_0210"/>
    <dbReference type="GeneID" id="10599650"/>
    <dbReference type="KEGG" id="aho:Ahos_0210"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="132" mass="15398" checksum="8397A3FED81D68F5" modified="2011-06-28" version="1">MKILIDTSFILPALGIDVGESVLNLIEEFDNHEIYYTELSLLEAMWMIRRLLKEGNNVDFKIVRTGLKSVFKTYHLLKIPISAYINAVNDMRHSDLIDMIIYYTAKAYNLKFLSLDKKLKELDKENIVIDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T9</accession>
    <name>F4B4T9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Creatininase</fullName>
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    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0212</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T9"/>
    <dbReference type="STRING" id="933801.Ahos_0212"/>
    <dbReference type="GeneID" id="10599652"/>
    <dbReference type="KEGG" id="aho:Ahos_0212"/>
    <dbReference type="eggNOG" id="arCOG04536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055029_3_1_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005:SF1">
        <property type="entry name" value="2-AMINO-5-FORMYLAMINO-6-RIBOSYLAMINOPYRIMIDIN-4(3H)-ONE 5'-MONOPHOSPHATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="3-DEHYDRO-SCYLLO-INOSOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Creatininase"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="234" mass="26586" checksum="90DF44E6C54CA87E" modified="2011-06-28" version="1">MHLLYSTRDEVFNEISLLPVGSIEQHGPHLPMGTDAIIAEEIAKRVESEFKDDVLLFPTIYYSCSIEHSGLPYVGVSYITFYNYLVEVIKKALELSKAVIIVNAHGGNQAILEVIKREINLNREDDKKESYNNKKVYVFYPDYNFFQGEDLHAGTVESSAIKYLYPSFVKESKVGKDFSVKEGVFNTITTAEANSQGIINIGEFKIDINIGELFIRHNVNKLKELVLKILKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V5</accession>
    <name>F4B4V5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF996 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775037.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V5"/>
    <dbReference type="GeneID" id="10599668"/>
    <dbReference type="KEGG" id="aho:Ahos_0228"/>
    <dbReference type="HOGENOM" id="CLU_1551726_0_0_2"/>
    <dbReference type="OrthoDB" id="43171at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19251" checksum="098378E35A8810E9" modified="2011-06-28" version="1">MEKVYSVIGAILISISFFLNVFGIITSAIGIYLVTSYIRSLYVEYKREKELGKFLYLAMSMLFLSFVSFSVGMYTAFGLKEFFVILPPSSVFSLAIIVYTYVLSGIFYIISAVYLIRSVTPLTVFTRIKGVSYSIYLMGISAVLLFSIVAAPGGVILSFVAWIWFAINLYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V8</accession>
    <name>F4B4V8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SirA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775040.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V8"/>
    <dbReference type="STRING" id="933801.Ahos_0231"/>
    <dbReference type="GeneID" id="10599671"/>
    <dbReference type="KEGG" id="aho:Ahos_0231"/>
    <dbReference type="HOGENOM" id="CLU_2613390_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="8948" checksum="006A1F2E7874C014" modified="2011-06-28" version="1">MIKRIYKRLDYSGVNSCDSAVGEILGYLDTLDKDEAIEIVLDADWKLKDLQDFISKTKYKILDVKKDDGKYIVTVGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B508</accession>
    <name>F4B508_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776225.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B508"/>
    <dbReference type="STRING" id="933801.Ahos_1427"/>
    <dbReference type="GeneID" id="10600919"/>
    <dbReference type="KEGG" id="aho:Ahos_1427"/>
    <dbReference type="eggNOG" id="arCOG01260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="OrthoDB" id="35501at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05344">
        <property type="entry name" value="BKR_like_SDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050259">
        <property type="entry name" value="SDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42879:SF5">
        <property type="entry name" value="SHORT-CHAIN ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="257" mass="28712" checksum="1F9F2A040C4DFA20" modified="2011-06-28" version="1">MKRALITASSEGIGKGIAKALAKQSFSLVITSRSEEKVKKAVEELRAINPSIWGKTSDMTNLKSLEELVSFSIDKMGGIDLLVVNTGNPSKEPSLFEETTDEDWEYSVKLYLLSAVKLTKLVLPKMKEQCFGRIIYLSSWTIKEPQDIFVLADVSRAPLIQLAKILSKDYGKYNITFNVILMGSFNTPGARRGIERLAKVRKKSFEEVWKEEVLDRIPLGRIGDPEKDLGKLILFLYDSDYITGTSILIDGGNTRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B555</accession>
    <name>F4B555_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776272.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B555"/>
    <dbReference type="STRING" id="933801.Ahos_1474"/>
    <dbReference type="GeneID" id="10600973"/>
    <dbReference type="KEGG" id="aho:Ahos_1474"/>
    <dbReference type="eggNOG" id="arCOG07209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431703_0_0_2"/>
    <dbReference type="OrthoDB" id="35924at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="20737" checksum="92642CD4A6FF9F3F" modified="2011-06-28" version="1">MSRLKQLLGEAVEEAEKYGSLLSTYLILKKCNAEYDTLVKEKEVNYSFSVDDIILTSLSYKELSKIQFFVMSFLVCDYLSSRYITQDCLFYFRWDKRIFIYSPRMEAHLLYLIRSGYAEGYKYFKLTEKGRVEAEAKKSLLSNVEKIKIDEISSCVLSKNKLSELKRYVRSYIFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B557</accession>
    <name>F4B557_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776274.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B557"/>
    <dbReference type="STRING" id="933801.Ahos_1476"/>
    <dbReference type="GeneID" id="10600975"/>
    <dbReference type="KEGG" id="aho:Ahos_1476"/>
    <dbReference type="eggNOG" id="arCOG07224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067653_0_0_2"/>
    <dbReference type="OrthoDB" id="36021at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="13712" checksum="7AA39C002595B1F6" modified="2011-06-28" version="1">MFEDLLKAVNYLNDGKILEAGEYLVELAKNNDANEDIIKISSEIEKELRELKEESWISEIDSKFRDQIISVLEDNIRCRKELIRVLSLSLLEKLSKGNELILNMIRNPHAESKPHTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A1</accession>
    <name>F4B5A1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Citrate synthase</fullName>
            <ecNumber evidence="6">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0304</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>oxaloacetate + acetyl-CoA + H2O = citrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16845"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 8">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="GeneID" id="10599745"/>
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    <dbReference type="eggNOG" id="arCOG04237">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025068_2_1_2"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:tricarboxylic acid cycle"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="CDD" id="cd06118">
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    </dbReference>
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        <property type="entry name" value="Citrate Synthase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450-Terp, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="2-MeCitrate/Citrate_synth_II"/>
    </dbReference>
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        <property type="entry name" value="Cit_synThplmales"/>
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        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
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        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
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        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
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        <property type="entry name" value="Citrate_synthase_AS"/>
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        <property type="entry name" value="Citrate_synthase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01800">
        <property type="entry name" value="cit_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041157">
        <property type="entry name" value="Cit_synThplmales"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK14037.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="CITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739:SF4">
        <property type="entry name" value="CITRATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001369">
        <property type="entry name" value="Citrate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="Citrate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001369-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="377" mass="43308" checksum="5BADAC3776728E5C" modified="2011-06-28" version="1">MSEVSKGLENVFIKTTSLTYIDGEAGILRYGGYDINDLVENCEYEEIIHLMLFGDLPSASQLQKIKEKINESYNVPYQVFEVLEKLPQDADAVGMLETAFSMLSSFYNYPWKKDENKWRAIEIIAKTSTLVSNIYRIKEGMKPRLPELSDSYARTFLETAFSRRPSDEEVKAMNATLIIYADHEVPASTTAALVTASTLSDMYSCMVSALAALKGPLHGGAAEATFEQLKEIGDESKVEEWFDKKIIKEKNRLMGFGHRVYKTYDPRAKIFKKYAEEIAKTPEAKKYLRIATKLEELGIKHFAEKRIYPNVDFYSGIVFYSLGFPEYMYTTLFALSRVLGWLAHIIEYVEEQHRLIRPRALYVGPIKRDFVPLKYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B3</accession>
    <name>F4B5B3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidoreductase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775123.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B3"/>
    <dbReference type="STRING" id="933801.Ahos_0316"/>
    <dbReference type="GeneID" id="10599757"/>
    <dbReference type="KEGG" id="aho:Ahos_0316"/>
    <dbReference type="eggNOG" id="arCOG01622">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_1_4_2"/>
    <dbReference type="OrthoDB" id="25239at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051317">
        <property type="entry name" value="Gfo/Idh/MocA_oxidoreduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708">
        <property type="entry name" value="CONSERVED EXPRESSED OXIDOREDUCTASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43708:SF8">
        <property type="entry name" value="OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="126"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="313" mass="35665" checksum="D7D6C918E7A58379" modified="2011-06-28" version="1">MKVAVVGCNGWGRVHLHALRKIGTEYYVFSRDEEKAKECMKKYEAKAYFTKYEDVLRSDTDIIDLVVSHDKHFEMGIEAMKAGKHLMLEKPIARTIEEAKGLINFSREKGVKFMVLEQFYFDSTVRKAKELLPNLGKLSLILVRSTHFNQPKEWRAVKEKMGGGALIDGGVHFIDTLLNLGGEYEEIKSFCRKYFSNMEGEDTTVASFKFTGGHLGLLIYSWSIKNPPKVPAFEIYGEKGSLVEDPNSRVPGKPYGDIIFNGERIEVEKVDPIEVEIREFLKAVENNTEVPMKPEIALRDLEAVLKIYDECNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E9</accession>
    <name>F4B5E9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ORF1 in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5E9"/>
    <dbReference type="STRING" id="933801.Ahos_0352"/>
    <dbReference type="KEGG" id="aho:Ahos_0352"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968242_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="58" mass="7206" checksum="66795AE9E9FDBD44" modified="2011-06-28" version="1">MFPFYIFEHWWELLQEENRYAKTKYKAPKEFNDFLHLFLPYRVEGVLSAKHIFTVSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J2</accession>
    <name>F4B5J2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1533</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776331.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J2"/>
    <dbReference type="STRING" id="933801.Ahos_1533"/>
    <dbReference type="GeneID" id="10601033"/>
    <dbReference type="KEGG" id="aho:Ahos_1533"/>
    <dbReference type="eggNOG" id="arCOG01955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460525_0_0_2"/>
    <dbReference type="OrthoDB" id="43520at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE94421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="313" mass="35269" checksum="E5442344CDC1957F" modified="2011-06-28" version="1">MFLFFYGVALPIGLLLTVLGLKGLMEITRYESGKTFLYKLNPLTKVFFGIIVMSVAAVTIWWIGAILTLVIMFLYLTLNDGKKKFAYITLLVFITIIGSTWSIAPYTPNSVLQMVFPCPISSYTVVWVWPSYFVNIMGYEPQLTLQALIYGAQISFRITSVLVSSLLLILTTTTSDIFRMFSKMKVPLAITFSILVGVKTVPKIFEILDTSVKMQFIRGLGYGKPRVLYPIYFFYAGLMAIIPSMVYLLRGAKNLAISVDTRGFRAYESRTMLVDLPFTKYDYIMFAVMAGLIILAVLANIYGFGRSIPYVGY</sequence>
</entry>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776354.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L5"/>
    <dbReference type="STRING" id="933801.Ahos_1556"/>
    <dbReference type="GeneID" id="10601057"/>
    <dbReference type="KEGG" id="aho:Ahos_1556"/>
    <dbReference type="eggNOG" id="arCOG07305">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933350_0_0_2"/>
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    </dbReference>
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        <location>
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            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="14436" checksum="73AC570F7C99BF64" modified="2011-06-28" version="1">MKIINTLFKYSFVDLIALISTFSLGFAKSFINFGILAESLLYLHISLALISFLLAVVLLKLSFNTGLEFPKIASTISFSSIVVAGSSGITFLLTNNNLFSHIMLGAFEVALISTSLLIGYLTCFFRLCKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N0</accession>
    <name>F4B5N0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Electron transfer flavoprotein beta subunit</fullName>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776369.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N0"/>
    <dbReference type="STRING" id="933801.Ahos_1571"/>
    <dbReference type="GeneID" id="10601072"/>
    <dbReference type="KEGG" id="aho:Ahos_1571"/>
    <dbReference type="eggNOG" id="arCOG00446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_2_2_2"/>
    <dbReference type="OrthoDB" id="6635at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF20">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN, SUBUNIT BETA (ETFB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="241" mass="26324" checksum="2B2EACB062CD01DF" modified="2011-06-28" version="1">MNIIALFKIVPDDTLIKISGNSLDLNVPNKISTYDKNAIEEAVRIKEKHGGKAYGITAGITDRKTIREALAMGLDEVISIDMKEMDVMTTAEAIAEEVNQIKPDIILAGESTTDSSGSVLPSYLAAMLNYPIVSYAKSISIEGDKVKIERNLTTLTEIVEAPMPAVISVVGEINTPRIPTVRQILESAKKPVRSLTFNKAPKVKIVSVSPYVIQRKKIIIEGKMEEAVDKLLSYLRGEGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P1</accession>
    <name>F4B5P1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated RAMP-motif protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775172.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P1"/>
    <dbReference type="STRING" id="933801.Ahos_0365"/>
    <dbReference type="GeneID" id="10599806"/>
    <dbReference type="KEGG" id="aho:Ahos_0365"/>
    <dbReference type="eggNOG" id="arCOG06024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867679_0_0_2"/>
    <dbReference type="OrthoDB" id="43325at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="2">
        <location>
            <begin position="12"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="320" mass="36857" checksum="10F334536E10C1C0" modified="2011-06-28" version="1">MMHYLVKVKNETPLIINYSSGNYYRSLDYIPSSSIIGALKAKILADKKIWGGENREELKMDLYVTDAYPSTAQEKQLNPPSLVTLYKKNEIDDYFIDSAKVIIETYQGKKKWGEEIIRLKKGPRHWYMKNGNVYPIQIERISTNILKLDDELKIAYQETKNNEKTGYLAHIESISPGNIFAFEAIGSEDQLNFLIDTLKEGIFVGSFKTKGYGFLKLLNYSEINGDKKSKNSLYILDFYGNVSYKYFQEIKSMLKRVIFESIGIDKRKRLNYNEWITDYTIRSGSVIIAESDRNLYELETESIIKSGGGKIVINHPVHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q1</accession>
    <name>F4B5Q1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775182.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Q1"/>
    <dbReference type="STRING" id="933801.Ahos_0375"/>
    <dbReference type="GeneID" id="10599816"/>
    <dbReference type="KEGG" id="aho:Ahos_0375"/>
    <dbReference type="eggNOG" id="arCOG07248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987722_0_0_2"/>
    <dbReference type="OrthoDB" id="42466at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="19"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14330" checksum="41B02DECCEDD2467" modified="2011-06-28" version="1">MGEDEALIDTSAFISNKRRKGKVSVITLIEFMEWALRKYMELSKKGEKLRALGYLNLIASLPINVGEVIEIKNDEIRDLIYYVVEKNLDPADAYLAVLSKKLKIPLVTDDKDFERVKDEIEVIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5T1</accession>
    <name>F4B5T1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phospholipase D superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0408</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775212.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5T1"/>
    <dbReference type="STRING" id="933801.Ahos_0408"/>
    <dbReference type="GeneID" id="10599848"/>
    <dbReference type="KEGG" id="aho:Ahos_0408"/>
    <dbReference type="eggNOG" id="arCOG02041">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066800_0_0_2"/>
    <dbReference type="OrthoDB" id="42630at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051406">
        <property type="entry name" value="PLD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856">
        <property type="entry name" value="CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43856:SF1">
        <property type="entry name" value="MITOCHONDRIAL CARDIOLIPIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase D-like" evidence="5">
        <location>
            <begin position="57"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase D-like" evidence="5">
        <location>
            <begin position="253"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="342" mass="37543" checksum="8FDE2503E92D43DA" modified="2011-06-28" version="1">MKPIVTLLVLIILSSISFIPTSYSSSSWYVKVQTCQVKIIVSPYNSTDIISCLCRGKYIYAEIYELTCCQIANAIINAVKKGGEVFVVLSSNVYGGIPSDEDQLVSELNSSGVHVKFLSGFQYVHSKVFVINNCTVILGSINPTYYGIHKDHGVDLDIHNSSIAQAFACIILDDYYGKPVNVDYPGIVVSPINSECELSDILSQPGHLYISMEELYPSSGFYPVIEQHERTVVTSTYSEDNCAVTQLGAVEIPDMTAKIIVVGNYVYVGSVNLDYNSIHCNRELGIIICNPAIAHSLESLICYWAQGKSAEGNSVYQLSNPLFLLLIIIILVAVAILLKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5U5</accession>
    <name>F4B5U5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0422</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
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        <property type="protein sequence ID" value="AEE93310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="10599862"/>
    <dbReference type="KEGG" id="aho:Ahos_0422"/>
    <dbReference type="eggNOG" id="arCOG03448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021555_0_1_2"/>
    <dbReference type="OrthoDB" id="43742at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
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        <property type="entry name" value="Uracil/uridine/allantoin_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618">
        <property type="entry name" value="NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30618:SF0">
        <property type="entry name" value="PURINE-URACIL PERMEASE NCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
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        </location>
    </feature>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="280"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="322"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="429"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="482"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="531" mass="59263" checksum="E2E568470EB2C3F0" modified="2011-06-28" version="1">MTEKLLDRVSITKFYPERGQVELNTVFPEEKLLWNADVHPIPVKNRNWGPTTYFLIWVSMVFIIPSWTLASVGLVFGLNILESISMVFLGNLIVLIPMIIQSHGGARYGLAEPQLTRSRWGIYGAIFPSWVRAIIGAGWWGIESYIITEAATAIYAILAGKLSVVAYTVSHYACYPFILSKDFPTIFWSTFIAVILAQIAVFYFSPINKSQPVLKWLARIGGPIILIAFIAVWAYFMSQVGWSANIFTLSPTTSSPSILSILGFLNANIAYWATMALTMPDYTRFAKSQFAQSLGQIPMPFLMLAVAIMGTMTTAAALKLYGQPIWDPIVLVTLHLSAPTSILILLAFILATFMVNVFANAVGPAYDIANTFPKHLSWFKGSLILIAIGLALGAWSYYGNAYSYIDNWLLTYGGLLGSVEGVIIFDYAVIRHFKFDLADIFWSKGRFRYWKGINPAAVITFIIVSAVLYLPYPGESIALDNAWVLSFLLSGAIYIPLMVFWVIPKYQKELKGSILTGYYSKETRKIFGVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5W6</accession>
    <name>F4B5W6_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5W6"/>
    <dbReference type="STRING" id="933801.Ahos_1581"/>
    <dbReference type="KEGG" id="aho:Ahos_1581"/>
    <dbReference type="eggNOG" id="arCOG03167">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773042_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF19">
        <property type="entry name" value="ARCHAEAL ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="9"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="146" mass="16987" checksum="37E15398B5E3FA5D" modified="2011-06-28" version="1">MRNLKSDDLHIVEQAIYELYGNVDYILFDEIQNIQGWEPFVSRLRKTKRIILTGSNSKLLSGELATSLTGRRVDFTLFPFSFKEFLRFKGVNYSEPLTTRERAEIKNYLREYMSIGGFPEALLLNSRQIVNSIYNDILFKDCNAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B641</accession>
    <name>F4B641_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF72 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0441</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B641"/>
    <dbReference type="STRING" id="933801.Ahos_0441"/>
    <dbReference type="GeneID" id="10599881"/>
    <dbReference type="KEGG" id="aho:Ahos_0441"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_0_1_2"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="entry name" value="Protein of unknown function UPF0759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
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    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
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    <dbReference type="PANTHER" id="PTHR30348:SF4">
        <property type="entry name" value="DUF72 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YECE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="TM1631-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="26830" checksum="0A460DB33A50C8A1" modified="2011-06-28" version="1">MEIYVGTSGWMYDWNPKKNLSWYVENSGFNAVELNASFYRFPTKKQVESWKKYKIRWAVKVNRIITHVKRLKDIEIWKKFEEIFHDLNPDFYLFQLPPNFKYSEDNLRRVREFEKEVGKKMAVEFRDVSWFNKDIKLEEATIVSIDSPIGVYIINNSGTVYLRMHGRDNWYFYDYSEEELREDAKRVISLSPEKLYVFFNNDLWMLDNGRKMLKILHELSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D4</accession>
    <name>F4B6D4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferritin-like domain protein, linocin M18-like domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1677</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Encapsulin nanocompartment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D4"/>
    <dbReference type="STRING" id="933801.Ahos_1677"/>
    <dbReference type="KEGG" id="aho:Ahos_1677"/>
    <dbReference type="eggNOG" id="arCOG05908">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067492_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140737">
        <property type="term" value="C:encapsulin nanocompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.10">
        <property type="entry name" value="hypothetical protein PF0899 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007544">
        <property type="entry name" value="ENCAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051429">
        <property type="entry name" value="Encapsulin_nc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37165">
        <property type="entry name" value="PEPTIDASE U56 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37165:SF1">
        <property type="entry name" value="TYPE 1 ENCAPSULIN SHELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04454">
        <property type="entry name" value="Linocin_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1284">Encapsulin nanocompartment</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00033738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="330" mass="38083" checksum="929137BF827C8CA3" modified="2011-06-28" version="1">MDKEEETRAIRGALMAELDAINYYLQQGRLFNKEFIKKVHEDIAKEEITHFGEFLRLLYENSKEDFDYIIKGWNEASKLIGSNVDFPLKVENTIVNKNPEHPENSLESFVYNALQKRKIRKIGKIIKYDEESISVYEIEEKDSEIVQKNDSTTYNLQFLNVQFNIRSDLPLNKVFNVAYKAGLKYSNKEDLTLLSSHPLSLLKSGKRENKSDWEVPGNIANDIIKAKEEIESLGYSDITVILSPIIYSKLFRVYDKSGVYEYELLRHCCEIETSPLITGAVVISKDSFYILEKDEAIVEFLGKEGVYSTYLISGKISPYLIDKNACIIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E6</accession>
    <name>F4B6E6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">OsmC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E6"/>
    <dbReference type="STRING" id="933801.Ahos_1689"/>
    <dbReference type="KEGG" id="aho:Ahos_1689"/>
    <dbReference type="eggNOG" id="arCOG03686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860694_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052924">
        <property type="entry name" value="OsmC/Ohr_hydroprdx_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35368:SF1">
        <property type="entry name" value="HYDROPEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="132" mass="14863" checksum="52CEA9BD2E7E3FDE" modified="2011-06-28" version="1">MEGRLEGDVAKTCVAGKEVDIGLMGSDYPTPEEMLLASALSCMMLTIYYVAKEMNVKLDDVEGYIEGTMDPKGFQGDPNVPPGLLEVNYFITVKSKDERINEVLEQSMRRCPLKDTLIRQIKVNVNWKVEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K3</accession>
    <name>F4B6K3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775329.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K3"/>
    <dbReference type="STRING" id="933801.Ahos_0525"/>
    <dbReference type="GeneID" id="10599966"/>
    <dbReference type="KEGG" id="aho:Ahos_0525"/>
    <dbReference type="eggNOG" id="arCOG04059">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184178_0_0_2"/>
    <dbReference type="OrthoDB" id="14842at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="71" mass="8431" checksum="415A53332A1F2DF8" modified="2011-06-28" version="1">MEPIKIKLSTGREVEINDDTVKILNKYVRTQMTLEELAKELGLNGWEEAYDLIKQVPAWIMWYPETIYKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L1</accession>
    <name>F4B6L1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0147 protein Ahos_0533</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0147 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775337.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L1"/>
    <dbReference type="STRING" id="933801.Ahos_0533"/>
    <dbReference type="GeneID" id="10599974"/>
    <dbReference type="KEGG" id="aho:Ahos_0533"/>
    <dbReference type="eggNOG" id="arCOG04308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165882_0_0_2"/>
    <dbReference type="OrthoDB" id="65304at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003319">
        <property type="entry name" value="PRK04330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158436">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="89" mass="9835" checksum="61D4CB379326A806" modified="2011-06-28" version="1">MATLYDNEAKIKQAVIILQKIVNDTSVPRNIRRAATDAIRNLQDPNLSAGIRAANAIGILEDISQDPNMPTHTRISIWNVVSILETVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L3</accession>
    <name>F4B6L3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glyoxylate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0535</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775339.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L3"/>
    <dbReference type="STRING" id="933801.Ahos_0535"/>
    <dbReference type="GeneID" id="10599977"/>
    <dbReference type="KEGG" id="aho:Ahos_0535"/>
    <dbReference type="eggNOG" id="arCOG01755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_2"/>
    <dbReference type="OrthoDB" id="7437at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000462">
        <property type="entry name" value="Glyoxylate reductase (NADP+)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF283">
        <property type="entry name" value="GLYOXYLATE_HYDROXYPYRUVATE REDUCTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="3">
        <location>
            <begin position="4"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="4">
        <location>
            <begin position="108"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="315" mass="35829" checksum="B1F68B13EA0A277E" modified="2011-06-28" version="1">MCNVLVTKKLPGNWIDYLKRECNVTLWEDIYPPPKEWILNNIEDKDGILITLTEKIDKEIIDRAKNLKVISTYSVGYDHIDIKYAKSRGIIVTYTPEVLTDATADLVFGLILAVARRICEGDSLIRKGEWKTPWYPTFMLGTEVHGKTLGIIGMGRIGKALVKRAKGFDMRIIYNSRRKHEEVDAEYVDLDYLLENSDYVVITVDLNESTYHLINEDRLKKMKRSAFLINASRGQVVDEKALIKALQEGWIKGAGLDVFEIEPLPKDSPLLKLNNVVLTPHLGSATVETREKMAEIAVKNLLLVLKGEKPIYEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L4</accession>
    <name>F4B6L4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA(Met) cytidine acetyltransferase TmcA</fullName>
            <ecNumber evidence="12">2.3.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tmcA</name>
        <name evidence="14" type="ordered locus">Ahos_0536</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a cytidine in RNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in RNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82211"/>
            <dbReference type="Rhea" id="RHEA-COMP:15704"/>
            <dbReference type="Rhea" id="RHEA-COMP:19834"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a cytidine in mRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in mRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15146"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a cytidine in tRNA + acetyl-CoA + ATP + H2O = an N(4)-acetylcytidine in tRNA + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53876"/>
            <dbReference type="Rhea" id="RHEA-COMP:13670"/>
            <dbReference type="Rhea" id="RHEA-COMP:13671"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>cytidine(34) in elongator tRNA(Met) + acetyl-CoA + ATP + H2O = N(4)-acetylcytidine(34) in elongator tRNA(Met) + ADP + phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43788"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TmcA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.193" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775340.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L4"/>
    <dbReference type="STRING" id="933801.Ahos_0536"/>
    <dbReference type="GeneID" id="10599978"/>
    <dbReference type="KEGG" id="aho:Ahos_0536"/>
    <dbReference type="eggNOG" id="arCOG01951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004652_1_0_2"/>
    <dbReference type="OrthoDB" id="312894at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106162">
        <property type="term" value="F:mRNA N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA cytidine N4-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01886">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024914">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 12" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="13">
        <location>
            <begin position="449"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="514"/>
            <end position="516"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="736" mass="84459" checksum="97A48A8355F34E2A" modified="2011-06-28" version="1">MKFNNLLQDFYDAKIGYYRHLAIILGKNYLDNAIELIKTYLQVNNNPKIAYAFHPWVNGSKDRLSTLKEAIMTNEIVDIDYSSSEKYLGESFDLTILDAVDDFRPNYISRLVDLTRGGGLVIIYSDDISDINKLYKFSLTRNGIVKNLFENRFIKMAINSRGIILVNDNDIKFKPFSSSEVSKPHKKSYDKTIPKSLYDLCLTDDQVKLIREFDFVLEKGKRILIVTAPRGRGKSAGVGLALSFYVKRTLSKPTNVIITSPSYYSSQAIIQFLEEGLRALKIRYKKKISKEGKIMKISAGEVNIKWTSPDLAKDENGDLIIVDEAAALGIENLEYIRNSWDKIVFISTIQGYEGSGKAFMKYINSLDNSQIVKLDYPIRYAKGDPVEKFIYNVMLLDAEPEVYRNNTQFMEITQEDLFSNEELLRQIYGILVSAHYRNSPDDLMFLGDMAFQRIFTIGYNAVAEIVEEGELDETTEDHILNGEENEGNLIPHRIIKYMRIRNFGKLKGWRIMRIAVTPELQNQGLGTKLLSNVEKIALKNNIDWIGSSFVADYKVLNFWIKNGFKPVYLASRKNEGLGGYSVIVMKALTERAEKFLVELGKLLKDKILRTSHQVYFNVNPRILAKILTNIEFKKEIEIDDTYIGKIYAYLNGKIPYNSASEAIHAIAEKYFYDLSFKLDEEEVSAIIARTFQGKSWSHSAKMLSVNNQEAENLLRRGIEKIVKKYYGQEEKDYIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Q1</accession>
    <name>F4B6Q1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ParB domain protein nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775377.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Q1"/>
    <dbReference type="STRING" id="933801.Ahos_0573"/>
    <dbReference type="GeneID" id="10600017"/>
    <dbReference type="KEGG" id="aho:Ahos_0573"/>
    <dbReference type="eggNOG" id="arCOG01875">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107444_0_0_2"/>
    <dbReference type="OrthoDB" id="89900at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16400">
        <property type="entry name" value="ParB_Srx_like_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB-like N-terminal" evidence="1">
        <location>
            <begin position="9"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="201" mass="23504" checksum="4D22B8A8B6D9788A" modified="2011-06-28" version="1">MTVANITPELIKINQIITHEDIDIVNLNKVINCIRKNKEIIPIIVDEESFMVIDGHHRFYAMKLLGFSKIPAYLINYRKDYVKVNKWFRKIVFSKGSNVDRILSLIIPNTEGKVCIKFFSRKFCSNSEYTLYWKLNIIEKYLLSIGVNVIKNPKEGIEPPSLDKEYVLSIAKKGLRFPPKTTRHSYEFIIPSYRISLNEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R3</accession>
    <name>F4B6R3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="12">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="12">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pheT</name>
        <name evidence="14" type="ordered locus">Ahos_0585</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775389.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R3"/>
    <dbReference type="STRING" id="933801.Ahos_0585"/>
    <dbReference type="GeneID" id="10600029"/>
    <dbReference type="KEGG" id="aho:Ahos_0585"/>
    <dbReference type="eggNOG" id="arCOG00412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020279_3_0_2"/>
    <dbReference type="OrthoDB" id="10073at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="13">
        <location>
            <begin position="267"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="539" mass="61036" checksum="D8AA8D3351712AE3" modified="2011-06-28" version="1">MVNIVLKESRLLNLLGLEEKDLNDVLFNLKSEVEKRGEDELEIEINSDRLDMLSPEGIKRAVDGILGRKLGEAKYEVVPTNYQLIIDDVESRPYALAAVVYDVKLDDDRIKELIQFQEKLHCTIGRKRKKVAIGIHDLDKIDGKVIRYSKVPLDYKFVPLNSDKEMSVSEVISSTEQGKLYGNISIKDNYSPAILQEDGQVLSIPPIINSNKTKIDSSTKNLFIDVTGTNFDAVAQTLDLLVSNFAEGGSKIGTVKVSRYTSSSPLLIHNKLVVKTSDINKRLGLSLSTEEIAKYVRMMRMDANINSDEIEVIVPQYRIDIMTYVDIAEDVAMAYGYKSFSLPQYTSKGMGELLPITKLERAFRGLAIGGGFQEIFTFVLNKSSYLLYNDFVKIQNPITVEYDAVRNSLIWNMLKFLAKNQHARFPIKVFEVGDVVVKSEESDTGYKNDTRACLAIMNSRVSYEELQSVVHQILYNLKGKEPRYVRQEKDYFIKGRSAKILFDNNVVGEIGEISPEILEKFNIENPVVIAEIYLEKLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R5</accession>
    <name>F4B6R5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0173 metal-dependent hydrolase Ahos_0587</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0587</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
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    </dbReference>
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        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="226" mass="25443" checksum="7785DEEEC61EB296" modified="2011-06-28" version="1">MPSLRWLGHAAVELNLQGKKIVIDPFIKNNPTAPVKLSYFDDFDLIAVTHDHYDHLGDTVEIMNKNKKALLFATWDLENYLNKEYNIPWDRMIPANVGGYVEYQGIKLALTQAIHSSEHSDPTGVIISADGITIYHAGDTGLFEGMKIIGEVFKPDYALLPIGGRFTMDPYQASIAVDMIKPKKGVIPIHYNTWDLIKVDPGDFERYVKDKGYKPIILQPGQSIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6V0</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">Ahos_1766</name>
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    <organism evidence="1 2">
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6V0"/>
    <dbReference type="KEGG" id="aho:Ahos_1766"/>
    <dbReference type="HOGENOM" id="CLU_1567237_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="142" mass="16487" checksum="962634CB49F904E5" modified="2011-06-28" version="1">MECPFCGFKYLIDVNVGQSINRRYINLGFSLLPFSVSTNLLRETSHLLSFKAEYCSNCKRIYTAWELNDLNNIDLDISKLNCRILKCYVIIPILSICPFHRYKKDIAKHFGLNVTDVQDPLIIIIWCRKNNDGILIRVRPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y9</accession>
    <name>F4B6Y9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1807</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776597.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y9"/>
    <dbReference type="STRING" id="933801.Ahos_1807"/>
    <dbReference type="GeneID" id="10601307"/>
    <dbReference type="KEGG" id="aho:Ahos_1807"/>
    <dbReference type="eggNOG" id="arCOG00761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_1_2"/>
    <dbReference type="OrthoDB" id="40408at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="142" mass="16168" checksum="C1A1A93D081AE299" modified="2011-06-28" version="1">MDCCFNYIGFVRREDNSASRKSIVKIVIKEEYVEGLKGVEEFSHLIIIYHLHLASSNKGLKRVRGGVEVGVFATRSQHRPNPIGISVVELVKREGNALYVKGINAFDGTPVLDIKPYDEWDSIPNPRVPEWHKMEMKSRVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B704</accession>
    <name>F4B704_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hexulose-6-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0599</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775403.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B704"/>
    <dbReference type="STRING" id="933801.Ahos_0599"/>
    <dbReference type="GeneID" id="10600046"/>
    <dbReference type="KEGG" id="aho:Ahos_0599"/>
    <dbReference type="eggNOG" id="arCOG00053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081825_1_1_2"/>
    <dbReference type="OrthoDB" id="15246at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033982">
        <property type="term" value="F:3-dehydro-L-gulonate-6-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019854">
        <property type="term" value="P:L-ascorbic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000022">
        <property type="entry name" value="3-keto-L-gulonate-6-phosphate decarboxylase UlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35039">
        <property type="entry name" value="3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35039:SF3">
        <property type="entry name" value="3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="225" mass="24178" checksum="ED412153E861FB25" modified="2011-06-28" version="1">MKDEVLERISSKKNLQVALDFISLDDAIRIAKMAIEGGVDIVEVGTPLVKAEGIRGMKQLREVAKDKILLADTKTADAGDVEVEIAKLGNADIMTVLGIMSDSTIKSAVDKAKELGIVVQADLINVNDPVKRAKRLKELGVDIIGLHVGLDVQKERGITVTSLKNEIKQISEFSLVSVAGGLNEKNIVDLLDLPINIYVVGGAITRSSDPLLVTKRLINIIGGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B772</accession>
    <name>F4B772_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776607.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B772"/>
    <dbReference type="STRING" id="933801.Ahos_1817"/>
    <dbReference type="GeneID" id="10601317"/>
    <dbReference type="KEGG" id="aho:Ahos_1817"/>
    <dbReference type="eggNOG" id="arCOG00321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="OrthoDB" id="98233at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF11">
        <property type="entry name" value="ZINC-DEPENDENT PROTEASE, TLDD_PMBA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="17"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="96"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="217"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="457" mass="51778" checksum="0C0C9E35A4A6E8F8" modified="2011-06-28" version="1">MDLEKVVKKFDSYKYLEARYHKNESSSIFLMNGRLLGVSKEKNEGYSVRAYEQGILYFASSTNAESLDLKKIQGQGWEELEDTDVYSGKYEVKQVKPFDSIPIDEKIKYFKDIYERIKSLDIKSKLVNFNIWYSESLEEKRIMFKDGSTVEGLVPRINFSYSLVLKYMDKTATVYSDEFGASGGLELLDSWKIGDKLEEKIKEVDKVLTKGKTPKEGKTDVVLSSLLAGIMAHESVGHPFEADRVLGREGAQAGLSYLKDLNIRKIGSNAVSVIDDPTLPNSNGFFLIDDEGVKARAKYLIKDGEVNELLQDRFSSPKFKTKSNGSARAMSFDREPIIRMSNTFFQPGDMSFEELIEDVKEGVYFKSYMEWNIDDMRLGERYVGLEAYEIKNGEIGDPLLFPVLEGKTTEFLPAIDAADRELKFYAGICGKGDPDQAIPVWLGGPDLRLRNVKVKVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B783</accession>
    <name>F4B783_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776618.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B783"/>
    <dbReference type="STRING" id="933801.Ahos_1828"/>
    <dbReference type="GeneID" id="10601328"/>
    <dbReference type="KEGG" id="aho:Ahos_1828"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_2_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07721">
        <property type="entry name" value="yflN-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF18">
        <property type="entry name" value="METALLO-HYDROLASE MJ0296-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="208" mass="23203" checksum="1D6F306A90DF948E" modified="2011-06-28" version="1">MKVYEIPLRFVKSFLIDYDKGYILVDAGTPGSGKRIAEFLNSKGVKLDYVVFTHTHPDHIGGAYELRNYTKAKFAIDANGVDYLREGKIREPVLHSAFLKVVFALGRPLFFKKFSGVKEDLILKEGELVEGVEVLRTPGHTNDSISLYLPSINSVIVGDTLQGTNKGLKYPSIYENLEELIKSVEKIKSLKPSFVYVSHGKSGSTFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B792</accession>
    <name>F4B792_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Archaeal Type IV pilin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776627.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B792"/>
    <dbReference type="GeneID" id="10601337"/>
    <dbReference type="KEGG" id="aho:Ahos_1837"/>
    <dbReference type="eggNOG" id="arCOG03871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1987484_0_0_2"/>
    <dbReference type="OrthoDB" id="375129at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13913" checksum="D641592F70008437" modified="2011-06-28" version="1">MGHSPALTAFIILIGIVASTFAVVYVAYSLVSSLFTQQSSIILYSGCVFENGTLKVNLFSPKQLTVSYVKINGEVFYPENQIMIYPGTNTYYINTEDRNLSPQSKVMVEIFFNNGEEISFNTTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A0</accession>
    <name>F4B7A0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776635.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A0"/>
    <dbReference type="STRING" id="933801.Ahos_1847"/>
    <dbReference type="GeneID" id="10601346"/>
    <dbReference type="KEGG" id="aho:Ahos_1847"/>
    <dbReference type="eggNOG" id="arCOG01619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19072">
        <property type="entry name" value="AKR_AKR3F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638:SF3">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43638">
        <property type="entry name" value="OXIDOREDUCTASE, ALDO/KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="30136" checksum="00F855B80F31ED8C" modified="2011-06-28" version="1">MKKFKYFTISDLALGTWKMGGGFWTPSYENDERDVNAIKKAIELGITTIDTAEMYGGGHAEELVGKAIKDFKRDDIFIITKVWPNHAKYEDVIKSAKASSSRLGTFIDLYLLHWPSSSVPICETMSAFEKLVEDGIVKFIGLSNFKPEGIQKANECLKKYEVTAIENHYSLLDRSDEDTLEYAKKNGIAYFSYTPLEKGRISEIKILNEIARKYNKTPIQVALNWYISIDVMIPIVKASNIKHLEENAGAMGWKLSKEDWEAIAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A6</accession>
    <name>F4B7A6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A6"/>
    <dbReference type="STRING" id="933801.Ahos_1853"/>
    <dbReference type="KEGG" id="aho:Ahos_1853"/>
    <dbReference type="eggNOG" id="arCOG05371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090553_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="195" mass="22516" checksum="7F709296A097F942" modified="2011-06-28" version="1">MMIYYQSSGLSYFTRFSKLTPKYYLFSLYFTMLITTTVVSVIMGLGVVSLFSYHFGETIAPKNWGLFFLDAILSRVFYLPLSLFLEELTIVTSRKLSNAISFIPIILAYLFGFSYININLGNLVYYSPFLSIQVLGMQSFFTRSIPLNFNDFKGPTLNVYYAIISLIGWSIILSFASMLLFRRLYYRSLEEARIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7E7</accession>
    <name>F4B7E7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AIR synthase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775468.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7E7"/>
    <dbReference type="STRING" id="933801.Ahos_0665"/>
    <dbReference type="GeneID" id="10600116"/>
    <dbReference type="KEGG" id="aho:Ahos_0665"/>
    <dbReference type="eggNOG" id="arCOG00637">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648312_0_0_2"/>
    <dbReference type="OrthoDB" id="42306at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02691">
        <property type="entry name" value="PurM-like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009186">
        <property type="entry name" value="Ni_metllenz_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF2">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006346">
        <property type="entry name" value="Ni_metllenz_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="1">
        <location>
            <begin position="93"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="2">
        <location>
            <begin position="212"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="420" mass="46354" checksum="F96A1E9867C2189F" modified="2011-06-28" version="1">MVDLEGIARRLPKDEARKEIIRWLEFYKGKRDINERIADSIITEVENSKKASIYEPVLSGIKAGDAGLGSRGIGDHIIHQKILEMAGKKEFEDAGIADDIIASIDGIHSRLSYFPFLAGFHATKATLRDIMVKGAEPLGVILDIHLADDVDVSYLLDFEAGVTTVTDFLGVPILAGSTLRIGGDLVLGERISGGVGAIGKLKSKFFARHNVREGMKIVMTQGNGGGTISATAIYYGFPEVIEETLSLDELVTCKIISNNPSIAESMTDVTNGGLRMDAIEISEANRLSFIIDSEKFLHLINPKVLKMLNELQIDPFGISIDSILIFTNNEDKVLELLNKNKIKADVIGYVTTYKGYPILTEDGKRLEPRFRESPYTPVKKVIGNYSPYTIDDIEKHLSNAVKFSKIRKENVLKNLKSHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7E9</accession>
    <name>F4B7E9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7E9"/>
    <dbReference type="STRING" id="933801.Ahos_0667"/>
    <dbReference type="KEGG" id="aho:Ahos_0667"/>
    <dbReference type="eggNOG" id="arCOG05889">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168027_0_0_2"/>
    <dbReference type="OrthoDB" id="42258at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="11243" checksum="11F7FA6CF727AA4F" modified="2011-06-28" version="1">MCEDRKCVEEVVDDYLKSLLRPQSTNEKFKLFQDNEEEDEEIDEKTVANSYLFTEILIKRILNADLDLDFIFEDLKSKLGNSHPIVIFLKELLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F5</accession>
    <name>F4B7F5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0673</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775476.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F5"/>
    <dbReference type="STRING" id="933801.Ahos_0673"/>
    <dbReference type="GeneID" id="10600124"/>
    <dbReference type="KEGG" id="aho:Ahos_0673"/>
    <dbReference type="eggNOG" id="arCOG00932">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053467_0_0_2"/>
    <dbReference type="OrthoDB" id="372128at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="GLYCYL-RADICAL ENZYME ACTIVATING ENZYME MJ0021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="32"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="340" mass="38898" checksum="321021C5336079FC" modified="2011-06-28" version="1">MLKGNPEISLYNRDLPLGCKYCRLGSKLVVFISGECGDSCYYCPVSEERFGKDVMYANEKKTESFQDFIYEAYKMNALGAGITGGDPILHLDKVVKLIEILKSEFGEDFHIHLYTSGRYVNYDALFALQRAGLDEIRFHPVRKEYLKAVEKALNFSFDVGLEVPAIPGEEEYLNFLIKWAEEHKVKFININELEITERNFYNLNSKGITISHGLAGGKGSFELALKVLEANVDSKLDIHYCSSVYKDVVETRTRFLRTIRYYSKPYEKYSGEGTIIKAFVRTNTDLSDYGEKASNGFYISPEFVDEVISKYNPDEVRIIEELPYGLKISEKLIYSKSKNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G6</accession>
    <name>F4B7G6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0684</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775487.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G6"/>
    <dbReference type="STRING" id="933801.Ahos_0684"/>
    <dbReference type="GeneID" id="10600136"/>
    <dbReference type="KEGG" id="aho:Ahos_0684"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_0_2"/>
    <dbReference type="OrthoDB" id="7186at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="294" mass="32407" checksum="4C9377A8BE3F14AE" modified="2011-06-28" version="1">MSDVRLLYLNDVSRKFAGFNKENSPFVILGIPMDITSSYRPGSRFAPSAIRDAAQYIEFYSLRTGVDMGEVGFNDVGDVALHPSNVEENVKRISDVVSYFSEKGKIVISIGGEHTITVGTASGTSADCIISFDAHLDLRDDYLGYKYDHACVMRRLSEKGIKIMEIGNRAVSKEELEYARKAGVPFITSWDVELLGYKEVARKIINFTEECKRIYITYDMDSIDPSYAPGVATPEPEGLKPTAILDIAKLIADKRIVGFDIVEVSPPFDPSGITSVLAAKIIMETAAIIKSKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H8</accession>
    <name>F4B7H8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_0696</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H8"/>
    <dbReference type="STRING" id="933801.Ahos_0696"/>
    <dbReference type="KEGG" id="aho:Ahos_0696"/>
    <dbReference type="eggNOG" id="arCOG01185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063953_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011460">
        <property type="entry name" value="PRK14879.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011463">
        <property type="entry name" value="PRK14879.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="25485" checksum="498ACA413FFC4503" modified="2011-06-28" version="1">MEELKLLKRGAESQIYETYFLGIHAIVKKRMSKAYRDPKLDRKINLERTIMEAKLMYNALKLGINVPAILYIDKDNFSIIMEFIEGITVKEVLWKNLYDPKKIGEMIGEIALRLHSSQIAHGDLTTNNLILKENKLFLIDFGLSKRTNDVEDFATDVHVFLRSLESVHPDKKDEVFDGFKLTYSKFKLYEKVMETLKDIRMRGRYVEERRSKSSNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L4</accession>
    <name>F4B7L4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0732</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the Lhr helicase family. Lhr-Core subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775535.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L4"/>
    <dbReference type="STRING" id="933801.Ahos_0732"/>
    <dbReference type="GeneID" id="10600186"/>
    <dbReference type="KEGG" id="aho:Ahos_0732"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="OrthoDB" id="33870at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="Lhr-like_DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="WHD_Lhr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="27"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="242"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="907" mass="103368" checksum="D622275A84672E09" modified="2011-06-28" version="1">MQEDLVFVDNLKKLGYEKLTPIQKLAIPIILKGNNTLIIAPTGYGKTEAAIIPIFYRIFASRPQEISTLYITPLKALNRDLESRLKRLGEAFGISIKTRHGDTTPKERKEIVEKPPDVLITTPESLQYIILNEFYRKYFANLRWIIIDELQEMLDEKRGYELSVVLQRLKRVIKGNPQIIGISATIGNIELAKKYLDINGNVEVVKIDVTKEMELKLLIPRPNDEIKDLALKEGLDIILTARLNEISKIIKENKPVLIFTNTRETAEFLANKLQSIFNLNVATHHGSLYRDVRINVEKDFKEGKLDAIVATSSLELGIDIGIISLVIQYMSPKQVIRLLQRIGRSGHSLYKKAKGVIIPSNYLFDILECEALIEAIGNYLEKPSYEENPLDVLAHQIAGMILEGYDDKKEILQIIRNSVYFANLKEEELDSVISLLESERIIKIKDDGKLSPSSRTWKYYYQVNMIPDSIRSYDVIEVSSNTKIGKLDEDFVVMLDEDSIFILGGKLWKVISIEDNKVIVEKASLKEGILPSWFGESIPVEKEIAKKVFKNLEKYMKESSPYEEVNEKLKKYKAKGYPELKSHEIIIEIIGQNKDLAVILSPFGSKGNNTLGAVISFILSKISGIKVTYRNDPYHIVLASMTPISREMMEKAIKIITSLSEREIIEIVNNAIKESPQFKWQLLIEAERFGAIDLNSDIQVSSTLLKAFTDTIVGQEAVKEMTVKYHDLSIFEELKHYSWNILEVPEPSPLSQDFLDRLLAFTTESNSAMLEIFKRRLLSKDVKVLCIMCGWNEIVSIRNVPEKCPKCNSIFLTVTTPDDKNSPEIIRKVLKGEKLKRLESKKLEELRTISSLFSRYGKYAFVALAVPGVGPSNAGRVLSKLSDGEEKFYLALIEEEKKFLRNSKYWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L7</accession>
    <name>F4B7L7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">H/ACA RNA-protein complex component, Cbf5p, C-terminal exon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L7"/>
    <dbReference type="STRING" id="933801.Ahos_0735"/>
    <dbReference type="KEGG" id="aho:Ahos_0735"/>
    <dbReference type="eggNOG" id="arCOG00987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:snRNA pseudouridine synthesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pseudouridine synthase"/>
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        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="2">
        <location>
            <begin position="138"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="221" mass="24891" checksum="E38C8581FA49C7C0" modified="2011-06-28" version="1">METHEKVDLPIVKKIAEEFKGTIYQRPPVRSSVKRRLRTRKVFDIEVLEGKDDKLFLLKISSEPGTYMRKICHDMGIILGTGAHMRELRRIRSGIFTENGLVTLQEVSEALYMWKNCKDETDLRKILLPMEIGLCGIPKIILDDNAVNAIAYGATANAPGIVAYQNFKKGDLVGLITLKGEGVAIGKALVDSNSVQQMKKGEVIKPNRILIKRDVYPRAWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L9</accession>
    <name>F4B7L9_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775540.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L9"/>
    <dbReference type="GeneID" id="10600192"/>
    <dbReference type="KEGG" id="aho:Ahos_0737"/>
    <dbReference type="eggNOG" id="arCOG07720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_511558_0_0_2"/>
    <dbReference type="OrthoDB" id="42451at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="531" mass="60280" checksum="95B8AC91E0AB5E32" modified="2011-06-28" version="1">MSQEELEEYEAEGNVEETEAALQEQNETQGYGISLDILNSLATIAAVLASRLGAFTNLVTDMKEVNEELKCNEKIPKRIKERRDDENYLKMTLPAVATNEDVRCEAENYPVCTLFRQGIMARTNCNGNNIMVYIGGASSFWRAGKRSHVSVYQFGAERRFPYNASMYQRMSELMKSKGVTGGYIVVLFGTERAKQEYVNPVNPIYPNTYESNQPPYSIIIPTYREIIKTIAQKKIMPIIRDSRGHCFCNACSGDAKSVSVLYPSFLLGLGNFFVVSQPPQFLQPRMYLVVGDDIDEYYVGSPIYSTGEECGDARISGFVLQAFSLDEYCKLYAQATGKSFNTSVKNGGVVLRIPPPNSWDCEDILNWSESLGYERSFLKAYINLAIAVGLILPSFPEDYQDEVEDYIYGSEFSNCQDLKECEDEVRIIANDIYNSLQSEDSDKLVSIVHHCHEESISVDKDCYNSCFSQFKDKIKRLSVWDYYHLGEDGKDELASIMAEEECKKVCSKFNVNKYEECMKNLYPSIRRHITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q3</accession>
    <name>F4B7Q3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1927</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776715.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Q3"/>
    <dbReference type="STRING" id="933801.Ahos_1927"/>
    <dbReference type="GeneID" id="10601426"/>
    <dbReference type="KEGG" id="aho:Ahos_1927"/>
    <dbReference type="eggNOG" id="arCOG01242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850511_0_0_2"/>
    <dbReference type="OrthoDB" id="44271at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_eL40_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L40E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="15204" checksum="01FC7048D247D956" modified="2011-06-28" version="1">MKACPRCGHLNPDNAYVCEKCGYYLGFESVPSIMPSTPIIPPEKPLVKVCPVCGYINPGDATRCQRCGYVFQQPQVIVCPRCGFENPINATHCLRCGYPLKQSVPQTIVQGRKFPTIPVLASVIIAYIMIIILVYVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S5</accession>
    <name>F4B7S5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="3">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1949</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776737.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S5"/>
    <dbReference type="STRING" id="933801.Ahos_1949"/>
    <dbReference type="GeneID" id="10601448"/>
    <dbReference type="KEGG" id="aho:Ahos_1949"/>
    <dbReference type="eggNOG" id="arCOG01601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="OrthoDB" id="296931at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03376">
        <property type="entry name" value="TPP_PFOR_porB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008818">
        <property type="entry name" value="PRK11864.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF1">
        <property type="entry name" value="2-OXOACID OXIDOREDUCTASE (FERREDOXIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="6">
        <location>
            <begin position="71"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="302" mass="33380" checksum="42458A0917269529" modified="2011-06-28" version="1">MTEVPSYIKEKKVIRQQYFLRGNAACPGCPIPKELDLLLEVMGKKTVLVVPASCSTVIMGDIHGSPSTVPVVHSAFASAAAIASGLSRQLKMRGEDAKVVVWAGDGATGDIGFATVSGAAERNEDILYVCYDNEAFMNTGIQRSSLTPYGAWTTTTPAGKREFKKPLPFIMMEHKIPYVATASIAYPFDYQAKVKKAKDIQGFRYIHLLSPCPPGWRFDSSLTIEVAKLAVETGIWPLFEVINGEFQLTGISKVLLDKSKRKPITEYLKLQGRFSKMSEKDIKVMQDAVDAMWDYIAELVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S8</accession>
    <name>F4B7S8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">pyruvate synthase</fullName>
            <ecNumber evidence="1">1.2.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776740.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S8"/>
    <dbReference type="STRING" id="933801.Ahos_1952"/>
    <dbReference type="GeneID" id="10601451"/>
    <dbReference type="KEGG" id="aho:Ahos_1952"/>
    <dbReference type="eggNOG" id="arCOG01603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="OrthoDB" id="372091at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051626">
        <property type="entry name" value="Oxidoreductase_gamma_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="4">
        <location>
            <begin position="11"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="181" mass="19991" checksum="ABC588DB14651479" modified="2011-06-28" version="1">MRWEIILRGRGGMGVVTAGETLVKAAVKEGKYGQSIPFFGGERRGAPVVSYVRLSDEPIYIHREIYNSDLVAVFDTSLFNIMDPLEGLKEDGILLLNTKTPKKLWKNTFYVDAVEIADKLNLSIAGWKLVNMPMVGAIAKVTGMIKIDSVVEAIEEEFSGKLGELNAEAAKLGYEMVSKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W5</accession>
    <name>F4B7W5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cytidine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_0756</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine + H2O + H(+) = uridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775558.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W5"/>
    <dbReference type="STRING" id="933801.Ahos_0756"/>
    <dbReference type="GeneID" id="10600213"/>
    <dbReference type="KEGG" id="aho:Ahos_0756"/>
    <dbReference type="eggNOG" id="arCOG04173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097262_0_1_2"/>
    <dbReference type="OrthoDB" id="39143at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055086">
        <property type="term" value="P:nucleobase-containing small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072527">
        <property type="term" value="P:pyrimidine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01283">
        <property type="entry name" value="cytidine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000008">
        <property type="entry name" value="Cytidine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050202">
        <property type="entry name" value="Cyt/Deoxycyt_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004064">
        <property type="entry name" value="PRK05578.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11644:SF2">
        <property type="entry name" value="CYTIDINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="13">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="47"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="14447" checksum="A7B8910F63A42073" modified="2011-06-28" version="1">MQETDLSDEKLLEIAKNATRNSYAPYSNIHVGAAIVTEDGKIFTGTNIENSSYGLSICAERVAVFNAVSNGYRKFKKIAIITSEGKGIMPCGACRQVLAEFSENMEVITLDKEGNVIRFKLNDLLPHAFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X4</accession>
    <name>F4B7X4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">5-formaminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0765</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775567.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X4"/>
    <dbReference type="STRING" id="933801.Ahos_0765"/>
    <dbReference type="GeneID" id="10600224"/>
    <dbReference type="KEGG" id="aho:Ahos_0765"/>
    <dbReference type="eggNOG" id="arCOG04346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065084_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006188">
        <property type="term" value="P:IMP biosynthetic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF1">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP biosynthesis enzyme PurP N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="IMP biosynthesis enzyme PurP C-terminal" evidence="11">
        <location>
            <begin position="160"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="348" mass="40110" checksum="5481CF97D0131C79" modified="2011-06-28" version="1">MIRISALASHSALDVFDGAKDEGFSTIALCKKGRERPYLEFKRIVDECILLNDFKEIPSESVQQKLISENAIIVPNRSLAVYVGYDNLENMKVKFFGNRKMLRWEERKGEKNYYKLLDEAKIRRPRIYTPDNIDSAVIVKLPESKRRVERGFFFAKNKEDFDKKLEELEKDGIIDEESIKEMVIEEFILGAYFNVNYFYSPIFNRTEIISIDRRIQSDWDEFYKLPADIQLSLNRTPRLIEVGHEPATIRESLLEKLFEIGYAFVEATQKLESPGIIGPFTLQLSVTPGLDIVVFDVAPRIGGGTNAYMGIGSQYSKLYFGKPISLGRRIAIEIKEAMNRSLEEKVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z8</accession>
    <name>F4B7Z8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose 5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775591.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z8"/>
    <dbReference type="STRING" id="933801.Ahos_0789"/>
    <dbReference type="GeneID" id="10600249"/>
    <dbReference type="KEGG" id="aho:Ahos_0789"/>
    <dbReference type="eggNOG" id="arCOG01122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_1_2"/>
    <dbReference type="OrthoDB" id="19013at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="227" mass="25235" checksum="43A02E9DF25804E9" modified="2011-06-28" version="1">MDAKEIVAKYSIDYIKDKKIIGIGTGKTIRKLIETISDIDIFKTKQYIASSIDSEIELSKRGFSVISLFSGIIPDVYIDSFDFLIRENNGKIVLIKGGGGALFREKLLTVNSKERIFIGEISKLKVQKILQIPIEIVPVSLSYVLTSLTSQGFRVKIRESQGKIGPLISDNGNIILDMEVDNTQDLCKLNNELSNIVGVIETGIFCDLYDTIILADTDGRLEIIKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B804</accession>
    <name>F4B804_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helicase HerA central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B804"/>
    <dbReference type="STRING" id="933801.Ahos_0795"/>
    <dbReference type="KEGG" id="aho:Ahos_0795"/>
    <dbReference type="eggNOG" id="arCOG00286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504016_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF2">
        <property type="entry name" value="HELICASE HERA CENTRAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA central" evidence="6">
        <location>
            <begin position="212"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="539" mass="62040" checksum="A0B4CEA12CAD514C" modified="2011-06-28" version="1">MNFNKIGPIALFILGFGLILLQYHLIYIYKFNSVYFVLIVPILISFIIIFLRKRNIISNILVKNNIFIIKSNNASIIGIAFRILGKQDINNSDNNLQKELDNLVDVLSRKSDNIKYSIVTYIYNRRKSSALIMFKECKNENYTMCETEVLQEYEILKKMAESIAPHIALEPIKVSNETLPIPNDFGNLTYARVFEKQYEIPKSESIQVDYDIELGYVITSSLAKIPIGIRSNDVFRHIGIFGTTGSGKSNTAAIIASQLASKGFNVIILDWHGEYIDKLKDFKIYNESNPLRLNILKLYNLEETVEIFQDVLELTDPQRYILYIILSELRKYKRFSLSTLYEAVSRIKDEYTWIKDVKLALLRRMNPILYRPVRLLLISNINMEDELANNNKIIIDLSFIPDVSVRRLYALFIIRAITDIYIKRKINNKPTLIVLEEAQNYFNNSNNEMVSRLLNEIRKFRIGLCIITQSPSSVSPDVVKNTNIKIIHAIKSNIDKKIIVDSLALDPNLSNILDKLDIGDAIISAPNIKQPTLIKIKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B807</accession>
    <name>F4B807_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Archaeal intron splicing endonuclease beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775600.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B807"/>
    <dbReference type="STRING" id="933801.Ahos_0798"/>
    <dbReference type="GeneID" id="10600259"/>
    <dbReference type="KEGG" id="aho:Ahos_0798"/>
    <dbReference type="eggNOG" id="arCOG01701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129670_0_0_2"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="14673" checksum="4875EF6601F7EC27" modified="2011-06-28" version="1">MTETEVEELLTKIMSSKISEKDLQELQMIYSNDKNFWNKISVSIDLRLRSKKAKISSKVDNLIYLYDSSGKVIGIVIIYNENEPLKINEIFKFLEIAKSSNVDAYLAIIDKYGDITYYSLSEVSLSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B829</accession>
    <name>F4B829_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1981</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776768.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B829"/>
    <dbReference type="STRING" id="933801.Ahos_1981"/>
    <dbReference type="GeneID" id="10601480"/>
    <dbReference type="KEGG" id="aho:Ahos_1981"/>
    <dbReference type="eggNOG" id="arCOG03696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022113_0_0_2"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051684">
        <property type="entry name" value="Electron_Trans/Redox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30176:SF3">
        <property type="entry name" value="FERREDOXIN-TYPE PROTEIN NAPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="497"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="522"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="556"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="591"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="633" mass="72409" checksum="F4E23F043614DBEC" modified="2011-06-28" version="1">MNNFLLLFGIAFSMLALMIYVIYEVEKWRSERRVLTALYIEGMMLTMNIGAYLYVTYHCLFYFLVINGAYAFLGLYSILSIKNIERKREVIYAIFSLFMVISEFFMGSLFVVLQTGKPATIDSSVENIWFVSVMLAEMSFTFYLSFRKLDKITRNYLLSLLLLMPWFPALFPNFVIPFWASAIIMIGATIMIYETLYNQRLRANQETFTTMELMAIYMLMMLGEFLYFIYSTLVVFDISMMIAMTWFIFRSLAGPNPIKGNYLRNSKLAFGIIFLTFVMEFFMGGTLDFVEGVFSPGISGFISSLSLPWLPLNSVQGAMWDFIDVVGSVLGSVWFLIMMGIEMGFLAFKKMMEMHVREVRVRMMLMIIAYAIYSIYIPTFSPISSISQYVPYLWSMGIGTLAPVSNSVLVGLIGTYVVYAILSFLFGSRNLCAVTCTAPLMYQGNFYDALKVYNRTSRLGKKTLTSKLSLWYKAIALGISIFVLASAIVSYLNSEGILHFTVYGVDITLLIYFIWFDIMWYILFISIPFLGTFACVTTGYCYWGVFNQAVSRIGLFRLKVKDPQQCLNCKTVDCATACPVGLTDMRASFIKNGEFRSMKCIGIGECVNACPYDNIMFYDVRHWLKEKFSKVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B874</accession>
    <name>F4B874_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-1 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B874"/>
    <dbReference type="STRING" id="933801.Ahos_2026"/>
    <dbReference type="GeneID" id="10601525"/>
    <dbReference type="KEGG" id="aho:Ahos_2026"/>
    <dbReference type="eggNOG" id="arCOG01189">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006533_0_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN ACTIVITY OF BC1 COMPLEX KINASE 7, CHLOROPLASTIC"/>
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="451"/>
            <end position="484"/>
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    <feature type="domain" description="ABC1 atypical kinase-like" evidence="3">
        <location>
            <begin position="78"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="486" mass="56133" checksum="56D98C1CFDB4A33C" modified="2011-06-28" version="1">MFRTFYVGFKLLPRVLMLRHYRRLTLENKKVNEKEIEKEAEKMLNALVSLGPAFIKLGQILSVHSDLLPEAYLKVLSRLQDSVPPAPWEEVYEEIKEDLGDELLKRLNINPSPIASASIAQVYLAELDGKKVVLKVNRPNIKKEVEEDIKVMKTLVPFLKYIFDESFYESAKAIIDDFSSRIFDEMDFTKEEFYMRKIKEELNFPDVKIPQPIYATKRVLIMEYVKSYKVTSEEAKKIIPPDTLSYKVFRTFMVMLLEKEYFHADPHPGNIGVDDEGNLVLYDFGMVGKMDKDTRNKLVRAYVALTRLDGIELVKVLDELGAIQPEADRELLAKGIELFLKTFQGVTPETLEVEDFISAANEVFYRFPLRLPQKLVLYIRTTSTLGGTCITIYPEFNFFSNLVKLIEDEDLVIPALIDQVKDTLSSFVRKFRTSLLERPVIERKNNTRGEYYLSLGIAILSILSYIFTKDITLALLLIIFALIIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B887</accession>
    <name>F4B887_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_2040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B887"/>
    <dbReference type="STRING" id="933801.Ahos_2040"/>
    <dbReference type="KEGG" id="aho:Ahos_2040"/>
    <dbReference type="HOGENOM" id="CLU_1943736_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14474" checksum="7C0CC2FF46B4FE5F" modified="2011-06-28" version="1">MLISISALVFYSQDHYKEVKLNPNDGYTMRINSPITFIYYKICGGNVSLKIINGSVINTYGAEGKYIALVKSNGQNSLLVFLNNNNQTISVVKYTIEALCSSKFYEFLFSVLLFIIGAGVVLVEIRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A5</accession>
    <name>F4B8A5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">rRNA adenine methylase transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0818</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775620.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A5"/>
    <dbReference type="STRING" id="933801.Ahos_0818"/>
    <dbReference type="GeneID" id="10600285"/>
    <dbReference type="KEGG" id="aho:Ahos_0818"/>
    <dbReference type="eggNOG" id="arCOG04131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_5_0_2"/>
    <dbReference type="OrthoDB" id="9883at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011489">
        <property type="entry name" value="PRK14896.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="218" mass="25352" checksum="42CAF5A46291A722" modified="2011-06-28" version="1">MKLSQNFLIDKFFIFKFSSYVKNDIKPVVEVGCGKGNISKVINPDLCIEIDDKFIKYLKSYNLIIADARFLPIKRGQIVSSLPYSITEDFFLEVSKLDQVISLVLILQKDFIDKILNYATYISFLLNYIFDIKTHEVIPPSAFTPSPKVYSIIVTFMRKRTYNAKVDEYLKCVSRFRNKKLKNVGEYCGFISNSEKRVREFKPCQVIELLNLMGLNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C3</accession>
    <name>F4B8C3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nucleotide excision repair nuclease, Xpf</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0836</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C3"/>
    <dbReference type="STRING" id="933801.Ahos_0836"/>
    <dbReference type="KEGG" id="aho:Ahos_0836"/>
    <dbReference type="eggNOG" id="arCOG04206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101253_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20075">
        <property type="entry name" value="XPF_nuclease_XPF_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053651">
        <property type="entry name" value="DNA_repair_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040956">
        <property type="entry name" value="Arch_Xpf_endonucase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="7">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="230" mass="26304" checksum="67CB8A1C588A4644" modified="2011-06-28" version="1">MLRIYVDNREKNSGIPEILKDIGISVIIEQLDVADYVLADGVAVERKSVSDLVNSVFDKRFFDQINRLTSAYETSFLLIEGNLNRIREITEKWKAINSALISIIVDYDVKVIYSSDKRDTAEVLVKLAEKFQEHGGKKRIINLHDKPKFESIKDIQLYVVESFPNIGEVNAKKLLEKFSTIRNICNASISDLERVLGSRKRAEELYKILITPYSPQSSNSNNKKSLMDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M6</accession>
    <name>F4B8M6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase DHHA1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776893.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M6"/>
    <dbReference type="STRING" id="933801.Ahos_2107"/>
    <dbReference type="GeneID" id="10601607"/>
    <dbReference type="KEGG" id="aho:Ahos_2107"/>
    <dbReference type="eggNOG" id="arCOG00423">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076809_0_0_2"/>
    <dbReference type="OrthoDB" id="18016at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052968">
        <property type="entry name" value="Nucleotide_metab_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42146:SF1">
        <property type="entry name" value="OLIGORIBONUCLEASE NRNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA1" evidence="1">
        <location>
            <begin position="224"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="325" mass="36842" checksum="8B82DCC288A558B4" modified="2011-06-28" version="1">MDYYAIVHNDFDGTASVAVYARAVKSLPKNVWFTEPTKVHNLLSKLEPRGVYNVMIADLGLNESTFDKIVESCKKLIDQGVKIQWFDHHVWKEEWKSKLSEIGVEVHHDTSTCGAGVVHKVMNPDDEFSSKLASADCSVDIWLHNDPMGEKLRRIVESNKDYGWKKKLIETFYNGILWNEEFQKILEDQVDQELKGYQKLPKYYRVIEINGVKVVVAIRWKGPPDISYAAQYLMTRTGAKVFVSANGKAISFRSSTINVRLFAAKLGGGGHPLAAGASLKIPLIYRILRRIGIISPANKWVCNVVTKVINDVGFKEYQQKDITQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R9</accession>
    <name>F4B8R9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="4 6">N5-CAIR synthase</shortName>
            <ecNumber evidence="4 6">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 6">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">purK</name>
        <name evidence="8" type="ordered locus">Ahos_0903</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + hydrogencarbonate + ATP = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="4 6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775705.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R9"/>
    <dbReference type="STRING" id="933801.Ahos_0903"/>
    <dbReference type="GeneID" id="10600375"/>
    <dbReference type="KEGG" id="aho:Ahos_0903"/>
    <dbReference type="eggNOG" id="arCOG01597">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_0_2"/>
    <dbReference type="OrthoDB" id="9299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="105"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="147"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="176"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="363" mass="41888" checksum="AAD2E6AADC37BC30" modified="2011-06-28" version="1">MSYQPLSKLKIGILGGGQLGWMLILEGRKFPFEFYVLGNKDEPACKIADKCFLEDQYKELVDSSDIVTFEFEHVSEKALDYAEEKGKLYPRINSVELKRERYKEKEFLRDHGFPVPRFYVANDGEEALKIVKDEFNNEGVLKQSRGGYDGKGQYFIHGDVDKYQFIKNMKCKFVVEEYVNFDFEASIIATRSRREFKAFTPTFNYNEKGILVYNYGPYYNDKMVEIADRLTKELDYIGTIGIEFFVRGNEVLINEIAPRVHNTGHYTLDGAETSQFEQHIRAIADLDLGSTRILSPSGMVNILGVGEVPKDVLKFGKVYWYGKSEVRKRRKMGHVNIIGDNLEDVKEKIDNVMKLIYPQGLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T1</accession>
    <name>F4B8T1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DSBA oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0915</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775717.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8T1"/>
    <dbReference type="STRING" id="933801.Ahos_0915"/>
    <dbReference type="GeneID" id="10600387"/>
    <dbReference type="KEGG" id="aho:Ahos_0915"/>
    <dbReference type="eggNOG" id="arCOG02869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102827_0_0_2"/>
    <dbReference type="OrthoDB" id="359198at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887:SF14">
        <property type="entry name" value="DISULFIDE BOND FORMATION PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13887">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13743">
        <property type="entry name" value="Thioredoxin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="223" mass="26076" checksum="D67BFD8B0DFD0D65" modified="2011-06-28" version="1">MIEIKFFHDVLCPYCFIATRRLMNVAKDYKDQVIVRHKSFMMISSIEDLKDIAPTIEEARELFKNEFSILKKYIPDYDPEKVINKGKIGYIWSLPPQMACKAAEFQKGDEGHWEYYKRAQEKLFFEGEDITSDDVLIEIAKEVGLDIERFKEDFKSKKAKLAVIQDEEEAHAMGIHGVPAVLINDKWLIRGVQTEDYYRQVIEDLLKNGGEPKTVNLKAYWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T4</accession>
    <name>F4B8T4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetate--CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0918</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775720.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8T4"/>
    <dbReference type="STRING" id="933801.Ahos_0918"/>
    <dbReference type="GeneID" id="10600390"/>
    <dbReference type="KEGG" id="aho:Ahos_0918"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF232">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
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    </dbReference>
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            <end position="60"/>
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            <end position="434"/>
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            <begin position="494"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="615" mass="69751" checksum="D40CE947ABB924B4" modified="2011-06-28" version="1">MNPIYTKMREIHEDSLRYPEKFWDAIARELFWYKLYEKVVEAHHPYYYWFKGGKINITYNILDIRKNSNKIALYWESENGEKKSLSYKQLYNLVSSFAGALTQLGLKKGDVVTIYMPMIPEAMVSMLACARIGVIHNVVFAGFGERALRERIEIAGSKAIITADVGYRRGKEISYLDVIENATKDLNLTKITVPRSGKVVGHNFYDLLDAKKVDAVLMDSQDPLFILFTSGTTGKPKGVVHSVGPYTVWAYYHVKWLLNFDDTSTFFSTSDIGWINGHSYSTYGTLLNNGTLLWYEGVPDYPDPQVWWRLIEDYKVTDIWTAPTAIRLLMKYGSKIDHDISSLRMIASAGEVLGEEAWKWLVELTSSKVYVIETWGQTENSGFITSPGGFYIGGIYYKKGSVGYPLPGIDIAIYDDNGKELPPNTKGNIVIKSSAPAFMSSLWHDDERYRKYYEKFGVYLTGDYGYMDDEGYLYILGRIDDVIKVAGHRLSPAEIENIVLSNEEIADAAVVGKKDEIKGNVLVVFASLKQGFLPSEELKNKIMSEIKKNYGGIAVIDDIIFVDKLPKTRTGKIMRRVLRALINNEELGDLSTLEEREAIEDLRKRLGEKIGKQGN</sequence>
</entry>
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    <name>F4B940_ACIHW</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B940"/>
    <dbReference type="STRING" id="933801.Ahos_2192"/>
    <dbReference type="KEGG" id="aho:Ahos_2192"/>
    <dbReference type="eggNOG" id="arCOG04263">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081191_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
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        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282:SF1">
        <property type="entry name" value="PANTOATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
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    </dbReference>
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        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
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    </dbReference>
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    <feature type="domain" description="GHMP kinase N-terminal" evidence="1">
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            <begin position="73"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="253" mass="28408" checksum="E18A4E211045649D" modified="2011-06-28" version="1">MLIPKKFNMLEVKVPLSISGVWYPVFSNDLEKSGSIGLTMVLEPYSRVEIKRGDAEIILNGKKIDFPNLDFLKNNLGNLHLEIKSDVPLGFGYGLSGSISLAYALGASEIFGIIEREAIKIAHESEVISKNGLGDVISQYIGGGLVYRKTPGIYGEVETIKIDWRPVCSKPLEKLPTISLLKNSESALFYIQDFLKERTIDKFFEVARKFTEELGFHSPYKKSFRKKGIIVRLDECEEGWTIHKPAMKGAYVI</sequence>
</entry>
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    <accession>F4B965</accession>
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    <protein>
        <submittedName>
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        </submittedName>
    </protein>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B965"/>
    <dbReference type="GeneID" id="10600444"/>
    <dbReference type="KEGG" id="aho:Ahos_0972"/>
    <dbReference type="eggNOG" id="arCOG08343">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2783801_0_0_2"/>
    <dbReference type="OrthoDB" id="40588at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="68" mass="8096" checksum="35BE97882A691B05" modified="2011-06-28" version="1">MDNTKLEEKILEILKNGAKTSEEIRNELIQENIDFTPLQFREVLAKMVREGKVKKIPNYERKKFLFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B976</accession>
    <name>F4B976_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fuselloviral integrase</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0983</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B976"/>
    <dbReference type="STRING" id="933801.Ahos_0983"/>
    <dbReference type="KEGG" id="aho:Ahos_0983"/>
    <dbReference type="HOGENOM" id="CLU_3371257_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="34" mass="3894" checksum="867556A4DFAE3F3A" modified="2011-06-28" version="1">MGDMPPQCSGRDLNPGHRLERPAYLTGLYYRSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>F4B977</accession>
    <name>F4B977_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved pro-fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B977"/>
    <dbReference type="KEGG" id="aho:Ahos_0984"/>
    <dbReference type="HOGENOM" id="CLU_3263578_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="41" mass="4583" checksum="3F70064F999306A2" modified="2011-06-28" version="1">MLSNVGLNTHSNRTISVELKEDGKDSSITQKTEYMILRSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B990</accession>
    <name>F4B990_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="274" mass="31217" checksum="573E091808E4B793" modified="2011-06-28" version="1">MKFLERKEGTLQLINNAPKAYPGFKNYVRKKIEEEEKLLEGVLFAEDIISAMHSGYKNALAGYLRSAEESNRFIIERASLSIFVSATEEKYLELLKERQWHILVDEGYIIRAASEGIGRIKKATGRTLKLNKYSVYLMGAPVCRKHLKFQKYSMSIDELVDNLRVRVSAKCKFCHRQADYFTLAMPKASALIGLAEYITGKSTTNLMRIYANISRVIHPYGFTDLDKDKVFTLWARDFLNIISEVNNLFNLVDSSRGSSNNGKLTGKFVKNGES</sequence>
</entry>
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    <accession>F4B996</accession>
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        <submittedName>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775805.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B996"/>
    <dbReference type="STRING" id="933801.Ahos_1003"/>
    <dbReference type="GeneID" id="10600476"/>
    <dbReference type="KEGG" id="aho:Ahos_1003"/>
    <dbReference type="eggNOG" id="arCOG04217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136010_0_0_2"/>
    <dbReference type="OrthoDB" id="26976at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043998">
        <property type="entry name" value="Put_Metallopep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18894">
        <property type="entry name" value="PhageMetallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative phage metallopeptidase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="14411" checksum="055422838AB8BFA9" modified="2011-06-28" version="1">MQVLKLEKAEDVKKLALRINKEANMGIDLNKVVFLRSRNSKTTAIARTLTLPPQWRYVLGEDILYIVEVVSEKFDNLECNDKIFVITHELMHIPRTMKGLRNHNYKGFKQIRRISKELAKELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D1</accession>
    <name>F4B9D1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D1"/>
    <dbReference type="STRING" id="933801.Ahos_1038"/>
    <dbReference type="KEGG" id="aho:Ahos_1038"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726883_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="377" mass="42516" checksum="D7335B721D8ECB44" modified="2011-06-28" version="1">MIFTLTFAIRASNNMLITTIPLISAYIFHFSSVLIGVLSSILSISSFISSFFVNSRLDNSRRRELFLISSATYAVVFPLFFVANCILIFILSFIAGFSLGILFPNIITLSGSLNDRKMSERMLALYTTALSLSLIISPGLESLILQRFSLREAFIFFSFLAFLVLFISLKVRFPESHERREISAKAREILFSPRFLAPFFNNLMYDIPFAFIEVFGGIYAIEYFHASYSMATFLFTLYFLTSFIGRALFTLRAPSNVFRIILLNVALSVIGLFLAFISFNVILYVISLLILGMPHGLTYPSSLVLIRRNFRDEERSAANSYFTGILMGLGGIVPIIMGGIVSLIGIRLSFLALTIVVIVFFSLFFIKQREIKNKESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F2</accession>
    <name>F4B9F2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2227</name>
    </gene>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777013.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F2"/>
    <dbReference type="STRING" id="933801.Ahos_2227"/>
    <dbReference type="GeneID" id="10601732"/>
    <dbReference type="KEGG" id="aho:Ahos_2227"/>
    <dbReference type="eggNOG" id="arCOG00644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="OrthoDB" id="24444at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="241" mass="26221" checksum="FC885162DCA05C9A" modified="2011-06-28" version="1">MIGKVYLVGAGPGDPDLITVKGLKILRQADVVIYDRLVSKELLKECKPNSELIYLGKGLGEAELQDKINSTLVEKAKEGKIVVRLKGGDPYAFGRGEEECVFVMEQGIPCEVIPGVSSAIGVPAYAGIPVTSRWYSSGFTVITGTRASDKIIDLDYIPKKGTIVILMGINKIDELQESLEKVRSSECPVAIIQNGTLPSQRVVITTLSRLKEAVRKENISSPAIIIVGEVVKLRNKLWKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L4</accession>
    <name>F4B9L4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved archaeal conjugative plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775846.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L4"/>
    <dbReference type="STRING" id="933801.Ahos_1046"/>
    <dbReference type="GeneID" id="41368733"/>
    <dbReference type="KEGG" id="aho:Ahos_1046"/>
    <dbReference type="eggNOG" id="arCOG09991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2985451_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043734">
        <property type="entry name" value="DUF5678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18929">
        <property type="entry name" value="DUF5678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5678" evidence="1">
        <location>
            <begin position="7"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="57" mass="6747" checksum="22C57582EF4B6924" modified="2011-06-28" version="1">MLIINDSRFVGKYVAVDSNDNIIGYADTREELVKNLEQKGYKMHEYAIIYVKEEKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N0</accession>
    <name>F4B9N0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Winged helix-turn-helix transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775862.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N0"/>
    <dbReference type="STRING" id="933801.Ahos_1062"/>
    <dbReference type="GeneID" id="10600536"/>
    <dbReference type="KEGG" id="aho:Ahos_1062"/>
    <dbReference type="eggNOG" id="arCOG05961">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204220_0_0_2"/>
    <dbReference type="OrthoDB" id="41721at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017009">
        <property type="entry name" value="UCP032840"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032840">
        <property type="entry name" value="UCP032840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12727" checksum="2B7F4F6A4C40530B" modified="2011-06-28" version="1">MELTPRLQDVINIVKQKGEINLKDLALELKVSPKTAKGYVRELTRLGFVEIDEKENVKLKVTQEDPVESLKKIIEIHESEINALKKEVEELKTEIIKLRKKNSRFATTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T2</accession>
    <name>F4B9T2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="7">ATase</shortName>
            <ecNumber evidence="7">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="7">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purF</name>
        <name evidence="13" type="ordered locus">Ahos_1114</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7 11">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T2"/>
    <dbReference type="STRING" id="933801.Ahos_1114"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="KEGG" id="aho:Ahos_1114"/>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_3_2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 7 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="7"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7 9">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 10">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 11">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="448" mass="49914" checksum="181984C5FFF95BD5" modified="2011-06-28" version="1">MKIKEHCGIFATIGENSVKYTYDGLKLLQHRGQESAGISYIEDGIKTIKGLGLVEEALNEKEVLQIKSKFSIGHVRYSTTGKTTLQEAQPLSNEKLSIAFNGTIANYFEFGNYSTDTEFILDFLSKDIKEGKDIVHAVKHFMDIADGAYSMVILDDKKRVLAVRDPKGFRPLVMGKIGESIVFSSEDSAIKQLGGKVLKDVLPGEIILVNPDGKILSDKVPSTSFHTCAFEYIYFSRADSKIDGVSVYMSRIRLGEILAKNHPAKADIVVPVPESSRPIAIGYSRESKIPLEEALIRTIVSKRSFIMPTNDKRKSVLEEKFGIVEDAVKGKRIVLIDDSIVRGNTMKRIIMLLRNSGAKEIHVRVGSPMIKYPCYMGIDFPSRSELIASNKDENEIAKILGADSIEYLTVDEMKQAIGRDSLCTACFTGIYPLKRNYSLQVMESVFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T5</accession>
    <name>F4B9T5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ordered locus">Ahos_1117</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775917.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T5"/>
    <dbReference type="STRING" id="933801.Ahos_1117"/>
    <dbReference type="GeneID" id="10600591"/>
    <dbReference type="KEGG" id="aho:Ahos_1117"/>
    <dbReference type="eggNOG" id="arCOG04421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061495_2_0_2"/>
    <dbReference type="OrthoDB" id="10775at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050089">
        <property type="entry name" value="SAICAR_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599">
        <property type="entry name" value="MULTIFUNCTIONAL PROTEIN ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599:SF3">
        <property type="entry name" value="SI:DKEY-6E2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="235" mass="27398" checksum="9E92E68F34DB43F2" modified="2011-06-28" version="1">METLIGEGKTKQVFSYDSDHVLLRFKDTITAGDGAKVDIMENKGVINAQTSALIFRLLERNGIRTHYVGMYDERTMIAKKLKMIPVEVVLRNIATGSIVKRLPIKEGEIFDPPIIEYFLKDDSRHDPMLNYYHLEYFKLMNRKEAEVVESVMMRVNEVLRDIIRKMGFELYDFKLEFGRLGNEIIVGDEITLDSMRVRDPKTGRILDKDLYRKGYDLKTVKEAYEEFLSKLEKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U5</accession>
    <name>F4B9U5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="14">HerA, homologous recombination repair enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_2290</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777076.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U5"/>
    <dbReference type="STRING" id="933801.Ahos_2290"/>
    <dbReference type="GeneID" id="10601798"/>
    <dbReference type="KEGG" id="aho:Ahos_2290"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053460">
        <property type="entry name" value="DSB_Repair_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040937">
        <property type="entry name" value="HerA_Thermprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="HerA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA barrel" evidence="13">
        <location>
            <begin position="3"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA central" evidence="11">
        <location>
            <begin position="119"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA-like C-terminal" evidence="12">
        <location>
            <begin position="348"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE95161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="492" mass="54990" checksum="541C8A04300DB60F" modified="2011-06-28" version="1">MIIGYVIGDATPQQATILAVKPVRLGLYVTMEYDNEKVLGLITNVTRGSPMIDGNTIDVGIVERLNRFDGKIPHYIKATVKLLYNLNSNSQPDLPPIAGTPVSLAEEEDLRKIFSEGDITLGRLIGTNVPVNIRVGALARHLAILAATGSGKSNTVAVLSQRIADIKGSVLIFDYHGEYYRSDIHPLHNIEPKLNPLYLNPREFATLLEIRGNAPIQYRIIRRAFTEYQKSVQEKIKSGDIDFEKLNQTFIKDLEDLIDDQEGKGAKKDSVDEVKNKLEEFADKYSGIIDLTSKDIISNLKTHSVNVVDISPLDEDAMDAIVSHYLRRILDARKEYRRKGTGLRFPVIIVIEEAHVFLSKNDDTLTKFWASRIAREGRKFGVGLIIVSQRPKGLDENILSQMTNKIILKIVEPSDKKYVLEASDNLSEDLVEQLSSLGVGEALIIGNLVKIPAFVKIDLFNGTLGGTDPDMRREWEKAEEEDKLHESLTDFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W6</accession>
    <name>F4B9W6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,4-dihydroxy-6-naphtoate synthase</fullName>
            <ecNumber evidence="3">4.1.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Menaquinone biosynthetic enzyme MqnD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mqnD</name>
        <name evidence="4" type="ordered locus">Ahos_2311</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of cyclic dehypoxanthine futalosine (cyclic DHFL) into 1,4-dihydroxy-6-naphthoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cyclic dehypoxanthinylfutalosinate = 1,4-dihydroxy-6-naphthoate + dihydroxyacetone</text>
            <dbReference type="Rhea" id="RHEA:33087"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:64254"/>
            <dbReference type="ChEBI" id="CHEBI:64270"/>
            <dbReference type="EC" id="4.1.99.29"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MqnA/MqnD family. MqnD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.29" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777097.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W6"/>
    <dbReference type="STRING" id="933801.Ahos_2311"/>
    <dbReference type="GeneID" id="10601819"/>
    <dbReference type="KEGG" id="aho:Ahos_2311"/>
    <dbReference type="eggNOG" id="arCOG00654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_945334_0_0_2"/>
    <dbReference type="OrthoDB" id="56581at2157"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13534">
        <property type="entry name" value="PBP2_MqnD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00996">
        <property type="entry name" value="MqnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003773">
        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030869">
        <property type="entry name" value="MqnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37167:SF1">
        <property type="entry name" value="1,4-DIHYDROXY-6-NAPHTOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="288" mass="32396" checksum="EFB986B42D4EB6D8" modified="2011-06-28" version="1">MVTIKVGALADSGDLYPFIPLIEGKIKPEGFNLEIEVIPTVQDVNEKVLKKEVDVSVPSAAMYPYIQDDYYIMSNAVASAVDGITGMPILASKEMSLDEVKKSRLIVHGPNTSAFTLYKLLIGKYGKLVIIKRVLDEIKGLGTEGDVLVAVHEIKMMYAMQKLGVKYYKIGSMWDMWKNVSNNAPMPMGMVVVSKELGKDLALKFKEVYEKSKKYAEKNLQEIIPKDVEIMREAQGANLDKEIVEKTIWADIQEYNVPENEVIKGLETFYRLTQEKGILPKVKKIDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X3</accession>
    <name>F4B9X3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2318</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777104.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X3"/>
    <dbReference type="STRING" id="933801.Ahos_2318"/>
    <dbReference type="GeneID" id="10601826"/>
    <dbReference type="KEGG" id="aho:Ahos_2318"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="258" mass="29524" checksum="9B50B561E4434A94" modified="2011-06-28" version="1">MKKRTPKLRELVEGVSSYYIIGNIVILSPKRKDIDKEKLAKAIMQINPKVKAVYIKRKVSGELRISELELIGGENISRTIFKENGLSFVVDVKKVYVNPTLGGERNKIKEEVKENEKILDAFCGYGGIAIHASTISRYVVAGDLNIEGLEMLRESLSLNKKKIHLIDIVQYDAHNLPFRDKVFDKVYADNPTMILEFLNELCRATKGELIIYILTTKEGISRIPAYKWLRVNDYSKDLFIFKGYIRCDDKNFSLITLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X9</accession>
    <name>F4B9X9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777110.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X9"/>
    <dbReference type="STRING" id="933801.Ahos_2324"/>
    <dbReference type="GeneID" id="10601832"/>
    <dbReference type="KEGG" id="aho:Ahos_2324"/>
    <dbReference type="eggNOG" id="arCOG09698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_971838_0_0_2"/>
    <dbReference type="OrthoDB" id="34472at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="293" mass="34046" checksum="0058DBC0E283962C" modified="2011-06-28" version="1">MQLADIISAITKVGGNPHAIAKITGISIINVIKSINILKYKYHLALFTGLLAENLQLQKILLLCKNSKIKLSTSKKLFFPLVNLFRADLEEDKFMLVIYTNKDALNSIQTAVKKLRDENIVECEIFEISETKRYYRDISCFNFDQNKWTCDRLLQFNKRGKIVKPDEEDINLIVKLQANPYIPYYLSPHYSHIKHVLQGFLYTLGINDTILDIIADKEVIFHPNTLWSAKIGNKYLMEIHINSKELDKIVQKIKDHCSEIFIVPETPSYAEGYSIPYEIFKEKKWEFPKIVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA02</accession>
    <name>F4BA02_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15 16">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15 16">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="19" type="ordered locus">Ahos_2347</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15 16"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777133.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA02"/>
    <dbReference type="STRING" id="933801.Ahos_2347"/>
    <dbReference type="GeneID" id="10601855"/>
    <dbReference type="KEGG" id="aho:Ahos_2347"/>
    <dbReference type="eggNOG" id="arCOG04045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_2"/>
    <dbReference type="OrthoDB" id="8674at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050165">
        <property type="entry name" value="DHAD_IlvD/Edd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21000:SF5">
        <property type="entry name" value="DIHYDROXY-ACID DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="35"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="366"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="NCBIfam" id="TIGR00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEE95218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="558" mass="59030" checksum="61042E0339F9577B" modified="2011-06-28" version="1">MIQKIRSEKVYGGYEKAPNRAFLRAMGLNDDDISKPLIGIAAAWNEAGPCNIHVLALANVAKEGVREAGGTPRIFTTPVVIDGIAMGSEGMKYSLVSREVIADTVELTVNAHGYDGFVALGGCDKTGPGLMMAIARLNIPSIYMYGGTTLPGNYKGKPIAIGDVYEAVGAYSAGKLSAEDLRIMELNAIPGPGTCGGLYTANTMGMISEALGVALPGSASPPAVDAERVRFAKETGKALLHLMEIGLKPRDIMTFEAFENAITVLMASGGSTNAVLHLLAIAHEAGVNLTLDDFDRISKKVPEIVNMKPGGEYVMADLHKVGGVPLILKKLLDAGLLHGDVITVTGKTMEQNLKEYRIPDVPHSHIVRDINNPYSPTGGIRILKGNLATEGAVIKASASKVKYHRGPARVFNSEEEAFRAVLNGQIKEKEVVIIRYEGPKGGPGMREMLAVTSAIVGQGLGESVALVTDGRFSGATRGIMVGHVAPEAAVGGTIAVVEDGDIITIDVDNGKLDVELSDKEIKERLENWKPPEPKYKTGLLAQYARLVSSSSKGAVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA27</accession>
    <name>F4BA27_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775940.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA27"/>
    <dbReference type="STRING" id="933801.Ahos_1140"/>
    <dbReference type="GeneID" id="10600614"/>
    <dbReference type="KEGG" id="aho:Ahos_1140"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF4">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="213"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="503" mass="57794" checksum="1391E1F177B3FDF0" modified="2011-06-28" version="1">MPNYDFIITTDRCLMTNHHHKEFLGFLGTGPAIGIPEKVWKWLACPKVKVDEYGRPIEAPYGMRKIEAKLIDEGFNAAIIDPDYIGKYTPTAKALLFSHHDYFAFGPPSSTWWGITKKEPVNYKSFQELINRPEITEGKKHGMKILVGGPSTWQWLWREDMIEKLGVNTLVDGEGEKVIVKLAQMILDGEELPKYIYVSGDDVPSIEDIPDIKGGSVNGLIEIMRGCARSCRFCSVTLRPTRYYPLDKIERELQVNVKAGIKHGVIHSDDVLFYGAVGILPKPEPLIKLHQLVKKYYKTIAWSHASLAAIRYSEEKYGLVSKLMEIVYQDGNQNYLGVEVGIETGSVRLAKEIMPAKSAPYKPENYPETVEEAFKIMHENHIIPAGTMIVGLPEETEDDVYRTIELVDNLRPYRSILVPMFFVPMGYFKNKDWFTRVKLNDAHIELYKKVFWHDVYWAEDIINKFYMKGPLYYPVKLTLKLFLSIAKRKMKQVEAMLESKLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA35</accession>
    <name>F4BA35_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="6">IGPD</shortName>
            <ecNumber evidence="6">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hisB</name>
        <name evidence="7" type="ordered locus">Ahos_1148</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775948.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA35"/>
    <dbReference type="STRING" id="933801.Ahos_1148"/>
    <dbReference type="GeneID" id="10600622"/>
    <dbReference type="KEGG" id="aho:Ahos_1148"/>
    <dbReference type="eggNOG" id="arCOG04398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_2"/>
    <dbReference type="OrthoDB" id="103579at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000003">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010121">
        <property type="entry name" value="PRK13598.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="193" mass="21648" checksum="ACBFA74D6A6AFC14" modified="2011-06-28" version="1">MSRYSTVKRDTKETEIEVYLDIDTPGNVEVSTPIPFFNHMLNSMLFHMNSSAKIIAKDKENYDDHHVVEDTAIVLGEAFKEALGNKSGIRRFFSIFTPMDEALVLVAVDISGRGMGIIDLDLKREEIGGLSMENVPHFFRTFAYNAGITLHIKKFSGENEHHIVEAAFKGLGISLYEASRIVNNRLPTTKGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA69</accession>
    <name>F4BA69_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775982.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA69"/>
    <dbReference type="STRING" id="933801.Ahos_1183"/>
    <dbReference type="GeneID" id="10600657"/>
    <dbReference type="KEGG" id="aho:Ahos_1183"/>
    <dbReference type="eggNOG" id="arCOG04283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204008_0_0_2"/>
    <dbReference type="OrthoDB" id="36613at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11624" checksum="983841DDF0653A5C" modified="2011-06-28" version="1">MKAKKLILVTSEAHPMHKAFLNVTDQLSKELGIEKEVKMEDYSFLADYGEKDDLGMSWLPQLFVELEDGKIVPILTKMPLGADLKADSTKGKEEALNKIKSLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA77</accession>
    <name>F4BA77_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH-ubiquinone oxidoreductase chain 4L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1191</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775990.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA77"/>
    <dbReference type="STRING" id="933801.Ahos_1191"/>
    <dbReference type="GeneID" id="10600667"/>
    <dbReference type="KEGG" id="aho:Ahos_1191"/>
    <dbReference type="eggNOG" id="arCOG03073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_1_1_2"/>
    <dbReference type="OrthoDB" id="44109at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004320">
        <property type="entry name" value="PRK05715.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="10211" checksum="34CBCB4A5B4D5ECD" modified="2011-06-28" version="1">MILGEFGVSLSITLIGIGIYGLINSRNIIRILLSSEIILNSTILLVFSISSLLGKVYSPIIFSVFAISMALIEVVVAFASIILYYRNKGSLEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L0</accession>
    <name>F4B3L0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GGCT (Gamma glutamyl cyclotransferase) domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774816.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L0"/>
    <dbReference type="STRING" id="933801.Ahos_0004"/>
    <dbReference type="GeneID" id="10599444"/>
    <dbReference type="KEGG" id="aho:Ahos_0004"/>
    <dbReference type="eggNOG" id="arCOG03271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085350_0_0_2"/>
    <dbReference type="OrthoDB" id="100169at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061929">
        <property type="term" value="F:gamma-glutamylaminecyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039126">
        <property type="entry name" value="GGACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510:SF4">
        <property type="entry name" value="GAMMA-GLUTAMYLAMINECYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510">
        <property type="entry name" value="TROPONIN C-AKIN-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13772">
        <property type="entry name" value="AIG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="2">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE92899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="31597" checksum="57282E10F7834261" modified="2011-06-28" version="1">MYLFAYGTLRYGFELHHLLKDSRFVGLGFIEGYKMYDIGNYPGVVRGDSIVWGEVYEIKDELISLLDEVEDYKGRPDDLYIREKTRVYFDQKRRYYIDNVNFYRYNHGIEFRDEIEPGDYSRWVGMPVIVNYFAYAENTNPEILKERGVKEILKEIEAYLEDYKIIFNIKCKYGLCANLKEFKGGKVYGYIYVMLEDELNSLDKAEEHLIKYVRDVIKVKDKQGKEYFAQAYISDYKEGEGEPTDFYKNSIIEGLKRKWDKISTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M1</accession>
    <name>F4B3M1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0015</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774827.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M1"/>
    <dbReference type="STRING" id="933801.Ahos_0015"/>
    <dbReference type="GeneID" id="10599455"/>
    <dbReference type="KEGG" id="aho:Ahos_0015"/>
    <dbReference type="eggNOG" id="arCOG00938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777602_0_0_2"/>
    <dbReference type="OrthoDB" id="30736at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023819">
        <property type="entry name" value="Pep-mod_rSAM_AF0577"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04084">
        <property type="entry name" value="rSAM_AF0577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273:SF2">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01104">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE92910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="351" mass="40077" checksum="5894CA42D3BE0FD4" modified="2011-06-28" version="1">MLWLVFTSGKCNLKCDYCGGSFPKDVVPWNVKYDINKLKNLIERDPEATVIFYGGEPLSNPKFIMKFLDYIKAKRYGIQTNGTLIKLLPENYWKKINVALLSIDGRKEITEKHRGKHIYDIVVKNAGYLKSLGIETIARMTVTQDSDIFKEVTHLLSLGIFDKIHWQLNVIWSDKWNVEKWAKESYLPGIKKLVDLFLEELKKGKVIKIIPILGVLSAYFFGGYKGSPCGAGYKSVSVTTDGRILSCPIAVWENWAVLGNVGSGFKLLEDPLPEICRRCEFKEYCGGRCLYSMKENYWGEEGFREVDEITKEYLKTVLSIIPTIKELIEQGIIKLSDLKYDPTLDSTEVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S1</accession>
    <name>F4B3S1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 10">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1225</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776024.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S1"/>
    <dbReference type="STRING" id="933801.Ahos_1225"/>
    <dbReference type="GeneID" id="10600701"/>
    <dbReference type="KEGG" id="aho:Ahos_1225"/>
    <dbReference type="eggNOG" id="arCOG04112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="OrthoDB" id="314at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000003">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="331" mass="38122" checksum="1E3E7FBC262BD9F5" modified="2011-06-28" version="1">MNYFFDEDKVIEEIKKRNAKKVLLQFPEGLRYFSTEIVSKLEDKIKDVEFIISGEPSWGACDIAEDEAQILGVDLIIHFGHTPYTWYYPKFPTVFIPTESNLKIEEKVIDEAKELAKKYNPRKISLSATIQHAKLLPELKEHFTEFEVEIGKPSNPFMLDGQILGCDYKAIPLDVDLHINISGGKFHALGIGLYTGKPVINIDPYSQRAEDLTNEVNKILKIRYSKIMQALDAKTWVIIQGIKVGQNRPLMVKYLEKKLKEKGYKVYVVSNKVLNVETLRNIDRSYIDAYIVTSCPRLPTDDLYNFEKPVLTPGEARMVINNKLEPYIFPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S5</accession>
    <name>F4B3S5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PNOB8-type integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776028.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S5"/>
    <dbReference type="GeneID" id="10600706"/>
    <dbReference type="KEGG" id="aho:Ahos_1229"/>
    <dbReference type="eggNOG" id="arCOG01247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299181_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="11721" checksum="96062C34BF299CC1" modified="2011-06-28" version="1">MSKLEEITDRSRKQLYLYLRGFDQRDKEIIIPDNVIEKITQALPIDQIYEVLHGFNPREVTINDAIAVITRAVQDPGFRNIFFMLLQNQFGEYLKETSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z0</accession>
    <name>F4B3Z0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdenum cofactor synthesis domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z0"/>
    <dbReference type="STRING" id="933801.Ahos_0059"/>
    <dbReference type="KEGG" id="aho:Ahos_0059"/>
    <dbReference type="eggNOG" id="arCOG00214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000006">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="1">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="149" mass="16363" checksum="3362439FA42B101D" modified="2011-06-28" version="1">MQKKEPIVDESGDIIKQEIITSGHRLVGYSLVPDDKVKILKAFAEALDNHEVDVIVSTGGTGYSKTDVTVETLRGIFDREVEGFGEVFRFLSYEDPQVRSAAYLSKATAGIIKDKVIYALPGSPDAVKLAMEKLILPEAPHLVFIARSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z8</accession>
    <name>F4B3Z8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FAD-dependent pyridine nucleotide-disulfideoxido reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z8"/>
    <dbReference type="STRING" id="933801.Ahos_0069"/>
    <dbReference type="KEGG" id="aho:Ahos_0069"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="3">
        <location>
            <begin position="22"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE92962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="345" mass="37348" checksum="AACB34A1339C574C" modified="2011-06-28" version="1">MLNSINLFTTGSTLYAMQEEIYDVVIVGAGIAGLSAALYTSRQRMSTLVVSKDLGGQLNLTQLIENYPAISADTGLGLASKVEAQAKKFGAKFIYGEEINGLRKEGDIFILKGLKGEYKARTVILAFGKSPRELKVPGENELKGKGVSYCAICDAAFFKDVPAAVVGEGDPGLDAVELLGKYAKPTYYISRSFQLAGEPDLVNSVLSNKNIQLFLGYRVLEIKGNSKVQGIVIENLKTKEVKELQVEGVIIEMGYVLNTDFLKGLIKLNDKGEIIVDDLARTSMEGVFAAGDITQVPYKQAVIAAADGVKAALSAYNYLRSKQGLPPVNVDWKAEKKKTVVSFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B450</accession>
    <name>F4B450_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase M24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1275</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776074.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B450"/>
    <dbReference type="STRING" id="933801.Ahos_1275"/>
    <dbReference type="GeneID" id="10600756"/>
    <dbReference type="KEGG" id="aho:Ahos_1275"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_0_2"/>
    <dbReference type="OrthoDB" id="1346at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01092">
        <property type="entry name" value="APP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050659">
        <property type="entry name" value="Peptidase_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46112:SF9">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="4">
        <location>
            <begin position="2"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="3">
        <location>
            <begin position="136"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="350" mass="38955" checksum="08FEE5DC99782E51" modified="2011-06-28" version="1">MRIKKLEELSQKYNNSCIIISGDPNVYYFTNYKGAGSIIYCDGVATLLAPLLEENRAREISGLDVKIYYPSNIANGILVGSLSDILPKLITKNTVALDINWSTVNVYRLLSSKYNLIDISKDISEMRSIKDDEELEKIKKAGEITSEAMKVSMEKILEGEITEKQLSGIIDYTMKASGAEDYAFPSIVAAGKNSSFPHHIPTDKKILENDNVVVDIGAKFDGYCFDSTRTFNVKGEVRKIYEIVLEAQLEAIDAVTSGVNASEIDKTARKVIEKYGYGRYFVHSTGHGVGIEVHESPYISFNSNDVLKKNMVITVEPGIYIKDKFGVRIEDTLIVTNGKPEVLETAYKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B455</accession>
    <name>F4B455_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776079.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B455"/>
    <dbReference type="STRING" id="933801.Ahos_1280"/>
    <dbReference type="GeneID" id="10600761"/>
    <dbReference type="KEGG" id="aho:Ahos_1280"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2679025_0_0_2"/>
    <dbReference type="OrthoDB" id="35291at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="74" mass="8640" checksum="3E23FD4982D208AB" modified="2011-06-28" version="1">MKEINSDGICPVVLLEIYREWKSLKEGEEEDVVIRTPWEASVQALEKWCNETGNIFLSYEKQGSKIVIRLKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B464</accession>
    <name>F4B464_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation initiation factor 6</fullName>
            <shortName evidence="3">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eif6</name>
        <name evidence="4" type="ordered locus">Ahos_1289</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776088.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B464"/>
    <dbReference type="STRING" id="933801.Ahos_1289"/>
    <dbReference type="GeneID" id="10600770"/>
    <dbReference type="KEGG" id="aho:Ahos_1289"/>
    <dbReference type="eggNOG" id="arCOG04176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="OrthoDB" id="33582at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000011">
        <property type="entry name" value="Translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003126">
        <property type="entry name" value="PRK04046.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="223" mass="24356" checksum="1171DF3CC4A683D0" modified="2011-06-28" version="1">MNIQRLSIFGTDNIGVYIFSNDKYTFVPKNLDNETKKLIQENLGTEIIETTIADSFLIGIFMTGNNKALLLPKNIRDDELKIIKELAKDIRVEVLNLKTTALGNVILTNDNAALVYPEFSDAEVKQIKEALQVDEIKKGRIAHVSVVGSVGVVTDKGGIVHIDATDDEIKELSKLFKINIDIGTVNFGSAFVRSGLVANDKGILVGSSTTGPEILRIQKALGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B482</accession>
    <name>F4B482_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776106.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B482"/>
    <dbReference type="STRING" id="933801.Ahos_1307"/>
    <dbReference type="GeneID" id="10600789"/>
    <dbReference type="KEGG" id="aho:Ahos_1307"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_5_2"/>
    <dbReference type="OrthoDB" id="14880at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF25">
        <property type="entry name" value="USPA DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="12"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="135" mass="14914" checksum="264E98AD871CA7A5" modified="2011-06-28" version="1">MSSPTYTVSLWFRRILVPVDGSENSMRALELATDFSLRYGSKVTVIHVCNTCEEAEHVKKKVEERIGDKIDYDFKIIKMSNDSSVPNEILKVINEDTFDAVIMGSRGTSVNSDINIGSTVLSVAANASITVIIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B492</accession>
    <name>F4B492_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776115.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B492"/>
    <dbReference type="GeneID" id="10600801"/>
    <dbReference type="KEGG" id="aho:Ahos_1317"/>
    <dbReference type="eggNOG" id="arCOG09962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120799_0_0_2"/>
    <dbReference type="OrthoDB" id="38991at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="134" mass="15458" checksum="03BC17AC570E27C0" modified="2011-06-28" version="1">MTQSLKPKSIKLLHYEEKKDNKQVFRQGVTLIEYEGQPSKIIQWSQLIEGDPFGDHEITYRINYGSESILRSFKVKYIGKEGDKHRILIKEGVSGCGTKTKRIENKEVLIPDKLYQPYPLQQKSEEGKDPGLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B496</accession>
    <name>F4B496_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FeS assembly ATPase SufC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776119.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B496"/>
    <dbReference type="STRING" id="933801.Ahos_1321"/>
    <dbReference type="GeneID" id="10600807"/>
    <dbReference type="KEGG" id="aho:Ahos_1321"/>
    <dbReference type="eggNOG" id="arCOG04236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_2"/>
    <dbReference type="OrthoDB" id="18492at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="4"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="246" mass="27074" checksum="D44FD64D5268A7EC" modified="2011-06-28" version="1">MSTLKIEDLHVEVEGKEIIKGISLEIKSGEVHAIMGPNGSGKTTLSLAIMGHPKYKITKGRILLDGEDITNLETDQKVKKGLFLAFQNPIEISGVKLSTLLVAEYNRVYGSNESVTQVFSKITSLSKSVGIADSLLNRGVFEGFSGGEKKRVEILQLQILKPKFAILDEPDSGVDVDGLKTISDVIKKVKEENNTGYLIITHYRRILDYVNADKIHVLYKGRIVASGSMELAKLIDEKGYEGVIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A5</accession>
    <name>F4B4A5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin protein Cren7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776128.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A5"/>
    <dbReference type="STRING" id="933801.Ahos_1330"/>
    <dbReference type="GeneID" id="10600816"/>
    <dbReference type="KEGG" id="aho:Ahos_1330"/>
    <dbReference type="eggNOG" id="arCOG04323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177997_0_0_2"/>
    <dbReference type="OrthoDB" id="23364at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="11819" checksum="33DF00B52539ED95" modified="2011-06-28" version="1">MPKRKQKEIDICPNCGTKVDKPVKTWNLVSPLPDAEGRITITVMGSYVCPNCGYKWRGVVSKIKAGGSSVEIQGKNGTVKKIGDEKPEKRDEGEIIELDLSDLDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D4</accession>
    <name>F4B4D4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774940.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D4"/>
    <dbReference type="STRING" id="933801.Ahos_0130"/>
    <dbReference type="GeneID" id="10599570"/>
    <dbReference type="KEGG" id="aho:Ahos_0130"/>
    <dbReference type="eggNOG" id="arCOG03852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1212561_0_0_2"/>
    <dbReference type="OrthoDB" id="56549at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="228" mass="26736" checksum="9E8191104D3BF683" modified="2011-06-28" version="1">MVDYLALLSSQNPYDRLDGWFKIDWLIQNNIVTKEKLIEMKDKFLNLLSYNDDTVKLHAWRMVPQLINKGIITVKDVKKYDFLSLLYDSEAWLLVKDLVNSGAIDIESVKKEKEKYIALLKGNELDRIASWSLILDIVNLGIIDKNDVENNKKYLLELFNFPAYDIRFNLLFLVAELISKGVLSPKELEPYEKKIEEIVKDKEFNQFVKIYEKDPRELESIGIHVFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4I8</accession>
    <name>F4B4I8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioesterase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774994.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4I8"/>
    <dbReference type="STRING" id="933801.Ahos_0185"/>
    <dbReference type="GeneID" id="10599625"/>
    <dbReference type="KEGG" id="aho:Ahos_0185"/>
    <dbReference type="eggNOG" id="arCOG00777">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_3_3_2"/>
    <dbReference type="OrthoDB" id="24516at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000113">
        <property type="entry name" value="Thioesterase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660:SF1">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="THIOESTERASE SUPERFAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="3">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="134" mass="14800" checksum="6424B435E807ABA9" modified="2011-06-28" version="1">MDFKVIENEAVASYLGMKIIEVKGGKSKLEIPYNERICRRGGVLHGGMIMTSMDYAGGLAVASINDGIDQVTQELKVNFLEPMYKGPFTVEAKVLRKGRTAVVVEIEFKDSEGKLGAVGLGTWYIIRDRKVSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K4</accession>
    <name>F4B4K4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="8">PRAI</shortName>
            <ecNumber evidence="8">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpF</name>
        <name evidence="10" type="ordered locus">Ahos_1351</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776149.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K4"/>
    <dbReference type="STRING" id="933801.Ahos_1351"/>
    <dbReference type="GeneID" id="10600837"/>
    <dbReference type="KEGG" id="aho:Ahos_1351"/>
    <dbReference type="eggNOG" id="arCOG01983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_3_0_2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="9">
        <location>
            <begin position="5"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="22253" checksum="87C5C4A79BD5AA41" modified="2011-06-28" version="1">MTIKLKICGISTISDILELSKMNIDYLGFVTDTISKRYVKDEFINIAKKISEKPIVSVKVNGKISELIEKAKYADLLQIHRVLSDSELEELNSYDKRIILYVPASEKYLAYLNKAIRYSDMILLDSPKKGVQIDLNFARKIAKEYDVGIGGGININNIDKVIEIDPKWIDISSGVEVYPGKKDTQKVIEIVRRVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L8</accession>
    <name>F4B4L8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776163.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L8"/>
    <dbReference type="STRING" id="933801.Ahos_1365"/>
    <dbReference type="GeneID" id="10600852"/>
    <dbReference type="KEGG" id="aho:Ahos_1365"/>
    <dbReference type="eggNOG" id="arCOG00302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072983_1_0_2"/>
    <dbReference type="OrthoDB" id="50465at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07432">
        <property type="entry name" value="PHP_HisPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF3">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13263">
        <property type="entry name" value="PHP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="205" mass="23187" checksum="5E7AC1AD9C6B1A46" modified="2011-06-28" version="1">MKFDFHTHTNYSDGKEDPKALVEYAKRKGIYVAITDHDTSKGYEKVKEEAVIPGEEVTTQFGHVVILCNFPPNPPKDISSLVDYSKENSCIIFPSHPFDIFRKGIGNRIFEYKFDAIEIFNSKAPKSANDKAYNAAKELKLPGLANSDSHVKEAIGSAYNEIEINEFNIDEILESIRKGKARPIGIGLSITAKFKILQWYIERKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q7</accession>
    <name>F4B4Q7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gatA</name>
        <name evidence="9" type="ordered locus">Ahos_1404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776202.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q7"/>
    <dbReference type="STRING" id="933801.Ahos_1404"/>
    <dbReference type="GeneID" id="10600893"/>
    <dbReference type="KEGG" id="aho:Ahos_1404"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="OrthoDB" id="7931at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="472" mass="51469" checksum="724C607483D2D034" modified="2011-06-28" version="1">MISKLVEDLKNGNLDPEEYVAKTFERINKLDKKINAFITLRDEEEVKKEVKESIKRGGKLAGVLIAVKDVISTKGIRTTCASKMLSDYIPPYDATVIEKLKNEGAVILGKTNMDEFAMGSTTETSYFGPTRNPWDLERTPGGSSGGSGAALAAGYVDLALGSDTGGSIRAPAAFTATFGLKPSYGTVSRYGLIAYANSLEQIGPMAKNAEDLGLLYSIIAGEDDKDATTIKYSVDNPPGEIPIKGIKIGVLSDILEMSDKSVVNVIRTAIDKLSSEGAIIEETKLGYAEYALPAYYIIAMSEASSNLARYDGVRYGYSKHFEGNWIETFSKNRGEGFGMEVKRRILLGSFILSAGYYEEYYIKALKVRRLIKNSLDQLFSKYDVLLSPTMPVLPPKIGEVINDPIKMYAMDVNTVIANLAAIPAISIPAGFYNNLPVGLQMMGRYLSDTMLIGISINIEKILNMHDLTAPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R3</accession>
    <name>F4B4R3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R3"/>
    <dbReference type="KEGG" id="aho:Ahos_1410"/>
    <dbReference type="eggNOG" id="arCOG07216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181225_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="9407" checksum="82ABFFDF1B216687" modified="2011-06-28" version="1">MLYRKFGYNVFNLGEALDVLKPFFSKKVSLSAIKYMQKIGLINKVDFLEYRLSNFDEYIYLMSIDYLKRRATLRHKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Y7</accession>
    <name>F4B4Y7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">gluconate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0261</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="EC" id="4.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the mandelate racemase/muconate lactonizing enzyme family. GaD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.39" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775069.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Y7"/>
    <dbReference type="STRING" id="933801.Ahos_0261"/>
    <dbReference type="GeneID" id="10599701"/>
    <dbReference type="KEGG" id="aho:Ahos_0261"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_2_2"/>
    <dbReference type="OrthoDB" id="42605at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047929">
        <property type="term" value="F:gluconate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000005">
        <property type="entry name" value="Putative L-rhamnonate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034599">
        <property type="entry name" value="Gluconate_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF2">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="D-GALACTONATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00008">
        <property type="entry name" value="gluconate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00179">
        <property type="entry name" value="mandelate_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="7">
        <location>
            <begin position="139"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="393" mass="44428" checksum="B4F5D8B5EFA75324" modified="2011-06-28" version="1">MKIQEIEPIVLTSKEKGGATWASTMILVKVTTSNGAVGYGEAVPTLRVISVYNAIKQVSKAFIGKEVEEVEKNYHEWYKQDFYLARSFESATAVSAIDIASWDILGKELGSPIYKFLGGKFRDKVLVYANGWYSDCVTPEDFANKAKDVVKRGYKGLKFDPFGQYYDWIDEKGLREAEERVKAVREAVGDEVYIMIEHHGRFNANSAIKIAHVLEKYNPLFMEEPVHHEDIEGYKKYRSSTKVTVALGERLISLKEAMFYIDNRLADVLQPDITNIGGVTIARKVVTLAEANDVEIAFHNAFGSIQNAVSIQLSAVIPNLLILENFYDWFPQWKRDLVYNGTPVEDGYVKVPEKPGIGVDVNEKLLEELKAEPIPLEVGEEPVWVVKNTWRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Z3</accession>
    <name>F4B4Z3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.77</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0267</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775074.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Z3"/>
    <dbReference type="STRING" id="933801.Ahos_0267"/>
    <dbReference type="GeneID" id="10599707"/>
    <dbReference type="KEGG" id="aho:Ahos_0267"/>
    <dbReference type="eggNOG" id="arCOG00976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="OrthoDB" id="33618at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="26416" checksum="775ED9A6B658E6EB" modified="2011-06-28" version="1">MTEKEEILRKIKNPELAEAFKKVDRAKFLPENLRSYAYTHVDEALPILPGINTTALSLGIYMLDELDLHKGQKVLEIGTGIGYYTALIAEIVDKVVSIEINDEMYKYSSRLLSEYQNVKLVLGDGTLGHEEEKPFDRVIAWASAPTLLCKPYEQLREGGIMILPIGVGRVQKLYKVVKKGYTPALENLGEVLFSRIRGLFGFYDDYEDDVESRLSRLERQVKSILSRLEKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B504</accession>
    <name>F4B504_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-directed RNA polymerase, subunit M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775085.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B504"/>
    <dbReference type="STRING" id="933801.Ahos_0278"/>
    <dbReference type="GeneID" id="10599718"/>
    <dbReference type="KEGG" id="aho:Ahos_0278"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406498_0_0_2"/>
    <dbReference type="OrthoDB" id="72957at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047723">
        <property type="entry name" value="TransFacS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase II subunit RPB9-like zinc ribbon" evidence="1">
        <location>
            <begin position="2"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="87" mass="9640" checksum="46B32FFB9B61D8D9" modified="2011-06-28" version="1">MKFCPKCGSYMKPKGNSMVCPKCGYKEEGVENITFTEKKSHDRDVTIVADGRRIKGGVALNLCPRCGNAVVIKIGKKYKCRACGYIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B561</accession>
    <name>F4B561_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">6-O-methylguanine-DNA methyltransferase</fullName>
            <shortName evidence="8">MGMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ogt</name>
        <name evidence="11" type="ordered locus">Ahos_1480</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = S-methyl-L-cysteinyl-[protein] + a 2'-deoxyguanosine in DNA</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776278.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B561"/>
    <dbReference type="STRING" id="933801.Ahos_1480"/>
    <dbReference type="GeneID" id="10600980"/>
    <dbReference type="KEGG" id="aho:Ahos_1480"/>
    <dbReference type="eggNOG" id="arCOG02724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_2"/>
    <dbReference type="OrthoDB" id="372118at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.70">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00772">
        <property type="entry name" value="OGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008332">
        <property type="entry name" value="MethylG_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023546">
        <property type="entry name" value="MGMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815:SF13">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02870">
        <property type="entry name" value="Methyltransf_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 8 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methylguanine DNA methyltransferase ribonuclease-like" evidence="10">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="9">
        <location>
            <begin position="70"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor" evidence="8">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="149" mass="16830" checksum="76397BD28B9DFA80" modified="2011-06-28" version="1">MIVYGLYKSPLGDITVAKSEKGFVMLDFCNCVEKNSLDNDAFSDFFEKLDKYFEGKEVDLREPLDINVNPFRLSVFKEVMKIPWGKTKTYREIAKILGTSPRAVGIALSKNPVLLIIPCHRVISEKGLGGYSRGIELKKKLLELEGIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5A4</accession>
    <name>F4B5A4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775114.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5A4"/>
    <dbReference type="STRING" id="933801.Ahos_0307"/>
    <dbReference type="GeneID" id="10599748"/>
    <dbReference type="KEGG" id="aho:Ahos_0307"/>
    <dbReference type="HOGENOM" id="CLU_1387509_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="22730" checksum="E432F68BB0B18C68" modified="2011-06-28" version="1">MMNKVIEDFLKADEIARKIYYHKFKKYVGLIYLLAGAYAPLTEAVILILKVSSLSTMSYILTGMFIAVLIPIFFINDRLLRLHGIISKMNKIIYKRSIYDQIYDIVMKILFVLVILGGILYHFCNFSSLVAFWILELWLLYTFFHLKFKMRVEDWVAMISPIGLILQAFTGSLMIIFVIGWITAGIISLVRAYGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B0</accession>
    <name>F4B5B0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NUMOD4 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775120.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B0"/>
    <dbReference type="STRING" id="933801.Ahos_0313"/>
    <dbReference type="GeneID" id="10599754"/>
    <dbReference type="KEGG" id="aho:Ahos_0313"/>
    <dbReference type="eggNOG" id="arCOG07327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727271_0_0_2"/>
    <dbReference type="OrthoDB" id="43780at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="153" mass="17608" checksum="136973069734FABE" modified="2011-06-28" version="1">MRRVKGYIGHVKIDKEGKVVESNVNNAEEIAKILKFNVEKGNQEAKELGFNKINGFAMFGSTKSLTFMKDTALLVDNKKADWQELFTTYTYIKSWLIGGIALLVLSLILYYLAIFTSYMDYFAPEPRFYTPTILLLISIFMIVLSKSKYSYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C0</accession>
    <name>F4B5C0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C0"/>
    <dbReference type="STRING" id="933801.Ahos_0323"/>
    <dbReference type="KEGG" id="aho:Ahos_0323"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF122">
        <property type="entry name" value="NAD(P)-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="240" mass="26191" checksum="F319265F503B63C0" modified="2011-06-28" version="1">MITGGTHGIGLVTAKLFAELGAQVIALGRNIREKNDKIQFVKVDLTSRSELLSFIDWYKKEIGKIDCLINNASVNSRYSILNVTLEEWDRMIALELTAPMLLSKMAAELMIKNGTKGKIINVAAIQAHYPLPESFPYATVKGGLISMTKSLAVDLGKYGIQVIAVVPGPIYTKGETVPESLDKRAATLLGRMGRPIEAAYLLAFLVSDYNTFITGSEIFIDGGRIISRKPDPEEITSGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C1</accession>
    <name>F4B5C1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldehyde Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0324</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775131.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C1"/>
    <dbReference type="STRING" id="933801.Ahos_0324"/>
    <dbReference type="GeneID" id="10599765"/>
    <dbReference type="KEGG" id="aho:Ahos_0324"/>
    <dbReference type="eggNOG" id="arCOG01252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_3_2"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008911">
        <property type="term" value="F:lactaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07145">
        <property type="entry name" value="ALDH_LactADH_F420-Bios"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000009">
        <property type="entry name" value="Aldehyde dehydrogenase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051020">
        <property type="entry name" value="ALDH-related_metabolic_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991:SF1">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="12"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="478" mass="52609" checksum="9A5D4DF0760C42F1" modified="2011-06-28" version="1">MLPIIGSERPDSENKIAVRNPATGEVIDYVPSLSREEVRKAIEIAYDSFPKLNSITPAKRSRALMEVASLIRENSEELALTMTKETGRPIKSSRAEIERTAQIFELASSEVKRALEGDFIPLDLYEYPAGNEKRIAMIKREPIGVVAAITPFNFPAASFAHKVAPALAVGNTVIFKPSSLTPLTQIKLAEIVLKKFPEGSINVVTGNSEMIGEEFVSNDKVSLITFTGSVSVGLDLASKAVRNGKRVIMELGGSDAMIVLEDADLDKAVKAALIGRFDYAGQFCNATKRVIVRKEIEEEFTKKLKEELRKFKAGDPMDETVDIPPLISEDAVKRMKDFLEDAIKNGGEKVFEGSVPNKGYYFPPVVLRLEPKSKARVLREEVFGPILPIVPVNTDDEAVNVANSTEYGLDASIFTSNFSRAYEIASKLKAGTVIINDTTRLRWDNLPFGGVLKSGIGRESVINTMLEMTETKLIVYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D5</accession>
    <name>F4B5D5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D5"/>
    <dbReference type="STRING" id="933801.Ahos_0338"/>
    <dbReference type="KEGG" id="aho:Ahos_0338"/>
    <dbReference type="HOGENOM" id="CLU_2079284_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="13886" checksum="E4D5877CF2376B62" modified="2011-06-28" version="1">MQKCRNNPNLSFLVNCRLLRNYNCHIKITLLIITKKFMHKAVDNGKIYLNRCECYTMEEYRGLLAIGYIFLFLSIFSLIFDYKVLSLDLVISFTMALSIFAIVYSIYKIRKESKEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I7</accession>
    <name>F4B5I7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta domain protein region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776326.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I7"/>
    <dbReference type="STRING" id="933801.Ahos_1528"/>
    <dbReference type="GeneID" id="10601028"/>
    <dbReference type="KEGG" id="aho:Ahos_1528"/>
    <dbReference type="eggNOG" id="arCOG07292">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754787_0_0_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43449:SF3">
        <property type="entry name" value="POLYMERASE NUCLEOTIDYL TRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="149" mass="17119" checksum="10A8D4EC20CEB706" modified="2011-06-28" version="1">MLNLSLENMELFRIATRKLIRDDVLAIIFFGSRVIGKYRKDSDVDVLIIIKKGGNRDFSKERLSFLKETGIRLDTTVFDEEQFEENLTLGTLMMGVSIAYCVTYDKIHAYEKINAFAEEVRKYNAVLELPYGKFIVGKTIRKCFGDLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K8</accession>
    <name>F4B5K8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved Acidianus plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776347.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K8"/>
    <dbReference type="STRING" id="933801.Ahos_1549"/>
    <dbReference type="GeneID" id="10601049"/>
    <dbReference type="KEGG" id="aho:Ahos_1549"/>
    <dbReference type="HOGENOM" id="CLU_2712578_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="72" mass="8478" checksum="B65476740D69C52C" modified="2011-06-28" version="1">MNKQKLEKFANLELDLLSVVLTLNDEDVESLIRELEAIIKKYKEKAIEYDIIQQISKLSDEEKKKLIEKLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5P9</accession>
    <name>F4B5P9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775180.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5P9"/>
    <dbReference type="STRING" id="933801.Ahos_0373"/>
    <dbReference type="GeneID" id="10599814"/>
    <dbReference type="KEGG" id="aho:Ahos_0373"/>
    <dbReference type="eggNOG" id="arCOG06103">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_634048_0_0_2"/>
    <dbReference type="OrthoDB" id="46262at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="47773" checksum="1AF25925FD4580A1" modified="2011-06-28" version="1">MECAVIPINRSDFTFESAFSAIVRTYELVRPEKFIILYAEFNDVHLRKLEKLFEELNSHHVEVVKKKLDMKSFDDFKKMIEEETKSCDKVFLIPTSGANIVAVYLTLLHAEDKGKYPLINYLFAFGPWIRYYYPFVPRSLEEKLLLGYSNIAGVNLDLNLEDYIERDLEFTAIIQLTAEKLNKFNSEEKDISLRVRGKEILNTSATSYDKAKKSLSMFIQDDELRENILKLAGAYKILVDGEEIEKVAYGRPLLIDTNLIYYGIHTHEIRDLVIPYCVHNEVLMTVNKKGKEEAFSNAVFMVYDTLRAKSRMLPSENTICDVAIPKIEADLIKNYLVVTSDKRAYQRWKRLAISRYADVRLANVEGERTSYAEVTSAIFNLAAILHFMKVEGVEVCIKNNCVKIDDMAKEVELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R9</accession>
    <name>F4B5R9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IS607 transposase protein TnpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775200.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R9"/>
    <dbReference type="GeneID" id="10599836"/>
    <dbReference type="KEGG" id="aho:Ahos_0396"/>
    <dbReference type="eggNOG" id="arCOG08402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058758_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="240"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="441" mass="51777" checksum="E012E7E424F53D5E" modified="2011-06-28" version="1">MLKTLKRTVRVESDFLNEWKYRVLREVEEYQRKIVNEMIEVILSEALPTTRKKLHERFYSDYKEKYPFLPSRVIEGAYVVAGRIVKSFRERKKEGLTRKDKPEYKRVVITIPNMINWRFNRISVSVLTHKGWVEIPLKFTKQFTRYVYEGWRVSQELKLRLIGRKVHLWLTFEKEVEVESKDGNYISIDVNENNVTLAVFEGFKLKELRRYETGLGRVVVNYSLRREEITKGHSTKDELVKKKLRKLRERERKLDILRKTVKRIVELARDLKAKVVVGKFSSRSKEKMEGNKSSKLRHRIHQWSIVKFVDMLKTQAIEVDEVSESYTSSINPFNGEKLKKRKQIVERVIKVFNPHLMTGSAHEGGGVKVLKVNARYLESGEVLLERDSIAPLNIARKVDGRVVVFPSTSPKDLRVTVYDPLRGVPVAELEVIKSKDKLRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5U1</accession>
    <name>F4B5U1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidohydrolase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5U1"/>
    <dbReference type="STRING" id="933801.Ahos_0418"/>
    <dbReference type="KEGG" id="aho:Ahos_0418"/>
    <dbReference type="eggNOG" id="arCOG00697">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736314_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052349">
        <property type="entry name" value="Metallo-hydrolase_Enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32027">
        <property type="entry name" value="CYTOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32027:SF0">
        <property type="entry name" value="CYTOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="14"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="150" mass="17239" checksum="B784794FB229ACCB" modified="2011-06-28" version="1">MYNEWLPVAFSLEGYPKRRGNAPIKEMMKFNVNVALGHDCIMDPWYPLGTGNMLQVLFMAIHMDQLTGVEELLNSINLITFNSAKAWRVKNYGIKEGNDANLLITNADDVIDLFRFMEPPKFVIKKGKIIAKDGKYVLFNGKWEEVKRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5W9</accession>
    <name>F4B5W9_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1585</name>
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    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5W9"/>
    <dbReference type="GeneID" id="10601086"/>
    <dbReference type="KEGG" id="aho:Ahos_1585"/>
    <dbReference type="eggNOG" id="arCOG07349">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1492906_0_0_2"/>
    <dbReference type="OrthoDB" id="38613at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="180" mass="21147" checksum="293135A32B4C6AAC" modified="2011-06-28" version="1">MTLEGREIQIIKANEDYAEKFYEYLQLLKDDPENYTIIRYDDVTLEDVKKIRWNDYPMFLAIEKEKVVGSIQVMRGKYFGALRQPHVAGIAYSVAKEFRGKGLIYALMFYALSNVSIVTAWVDERNIRSQKVLEKLGARKIGKIDDFMYSIREKSFVNMIFYVGNAEEMRRKAKEEAERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5X2</accession>
    <name>F4B5X2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1588</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5X2"/>
    <dbReference type="STRING" id="933801.Ahos_1588"/>
    <dbReference type="KEGG" id="aho:Ahos_1588"/>
    <dbReference type="eggNOG" id="arCOG02666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773043_0_0_2"/>
    <dbReference type="OrthoDB" id="148218at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="146" mass="16582" checksum="714BC97EC61069E4" modified="2011-06-28" version="1">MTTPKAFFLGDLFEGKLTPYLSGIIDSGDYANINEKKDEVCKILNFYLLNAGSGEITEYVRSIITAFLEDNDYTKALRMMSEHIKDHSKCCEQFISSKKYFTEIFQEGRIIVTPAWHVSISAALNRGLLAELNLINKHKGKLIRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B649</accession>
    <name>F4B649_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775253.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B649"/>
    <dbReference type="STRING" id="933801.Ahos_0449"/>
    <dbReference type="GeneID" id="10599889"/>
    <dbReference type="KEGG" id="aho:Ahos_0449"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_711011_0_0_2"/>
    <dbReference type="OrthoDB" id="37037at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="42154" checksum="AA5E10251F0545DA" modified="2011-06-28" version="1">MDRFNKIFIINNGFSAFGLNLIDMTLLWILYLALKNPILYIPVASAKPIGTLLLSSFTGYIADKYDRKKLFAITGTIHRILPIFIVYFVIISFNPILAVILYVVRTGLTIVTTNTITPSFIQNLPEDKMKKVMFYNRAVKESSTILSIVIWPLFYAFYSGWTVIPGIILATIGFALIFTLPLGGKGGKKVKFREALKEYRRKEIFFISMTTAVSQGALGMVYLYSAAIIQILHGNTIQYTLAQLGFYLSWLIGPWMMTKFNNVTKLVVTSFAMYFLVFIVLALKNPWLIGLSTGSVGIGDSLTEIIWIKAMRMARQELMASVLGIDEFITAGFRLGYGSIAGTLYATDFFAVPIIGVITIAGVALAYTKNDVWKMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>F4B667</accession>
    <name>F4B667_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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        </source>
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        <source>
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    </evidence>
    <sequence length="65" mass="7490" checksum="1984924EC5FECE4E" modified="2011-06-28" version="1">MLISLLELLIILKLYFSQITTKDIENVFKNISLFVYYLTRNNSCNNDNIGSSNHSCDYVLLQTAL</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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        </source>
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    </evidence>
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    </evidence>
    <sequence length="307" mass="34315" checksum="3F7E79F0BA7B305E" modified="2011-06-28" version="1">MLENYLKWDSLFDQAIKEAKEINFEIKTDKVIFTGMGGSYIPGKIAEILDYNVDYRAITGIPRKIDENTTLIAMSYSGTTSETIQAVKEGLEKGSKIVVISSDGNLEKIAEDHKEKIYFIKVKGLSQTRYSFPVLITPLLKILSTVSINKINLDELKEGVIENKEKYKDLGKELSTKIIGKIPVFYASTYFPIAIRFKQEINENAKSPAFYGEIPEVNHNEVESYVRGKDLLLPIVIGNESIDDVTAGVLNAERIIPPSSSKLKNISSLVLLAGLTSIYLADTLKENPEKLSIIPRCRERTSVIFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="11340" checksum="C33D9F566FEC31B6" modified="2011-06-28" version="1">MFEIWYIHITLAIISVIVSILIFTEFYKLRREFNRSRIFSVLLVVSIFLTLDSVSDLFAFSMWSSNRNPVIIYPSLAISIFTTLSIILLYYYITRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H8</accession>
    <name>F4B6H8_ACIHW</name>
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        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
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        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
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        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
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        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="17"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="453" mass="49168" checksum="11DFA9B3A339B690" modified="2011-06-28" version="1">MTDLEKGAVGFRELLGQSIALIAPLGAVAATLTGAAQFALGSLPLSYLIGIFGVLFWINVPYQFSRKIGSAGGFYDFAEKGIGKRFGIFTGYLYLFSYFNVITNSIIFVGGVFIPSMLSQFFNITLPSLIWIPIMLIFLALITTLAYLGIRPSLRYSLITSIIEIALLVIFSIIIIVKAGPRNTLEPFTPTPAGGWAPVFEGMILAIFSMSGSSGAVYIAEETKNPLKDVKKAVLISFLITGVVFVLTSYAMVIGWGINNMSTFAPSGVPGLILADKYVGPIFVAILLAFILNSFFAGSLAPLNSASRLLYALGRDNVAPTYVSKVHEKFKSPSNAILLLGLLSGVVSLITGLIMGPFYGFLFLINMSAISLFIGHMLGDVALPFFYKKIREFNVVYHLIAPIVSFIILIFGIYYSFYPPTYPINYSAIATAIYSLGVLAFIFSRKVKVSQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M1</accession>
    <name>F4B6M1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="12">TC-AMP synthase</shortName>
            <ecNumber evidence="3 12">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_0543</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775347.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M1"/>
    <dbReference type="STRING" id="933801.Ahos_0543"/>
    <dbReference type="GeneID" id="10599985"/>
    <dbReference type="KEGG" id="aho:Ahos_0543"/>
    <dbReference type="eggNOG" id="arCOG01952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_0_0_2"/>
    <dbReference type="OrthoDB" id="39992at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11030:FF:000001">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000008">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="14">
        <location>
            <begin position="14"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE93431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="351" mass="38833" checksum="BED8D8D8CBB3C625" modified="2011-06-28" version="1">MTLILKVDPLNPEIDKIREAAKYIKLGGIVAFPTETVYGLGGDAYNPEAAIKVFKAKNRPMDNPLIVHIADLNQLFDVAKDIPDIVLDMAQKVWPGPLTFILKKTDKVPKETTGGLDTVAVRMPAHPIALQLIRESGVPIAAPSANLATKPSPTKAEHVIADLNGKVDVIIDGGDTFFGVESTIINMTVNPPVLLRPGPFTLEELHQFLPEIVIPEQLIKGGEFTVALAPGMKYRHYAPSKKLLLVENYSIFKDVVKLLRSKYKVAVLCTTEDFKDFEEPRIILGSRENLYEIAKNLFDSFRKLDRLDVDMGVMEGVPEKGIGFAIMNRARKASGFSIIKTLDDVKKYVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N3</accession>
    <name>F4B6N3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Molybdopterin-guanine dinucleotide biosynthesis protein A (MobA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0555</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775359.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6N3"/>
    <dbReference type="STRING" id="933801.Ahos_0555"/>
    <dbReference type="GeneID" id="10599998"/>
    <dbReference type="KEGG" id="aho:Ahos_0555"/>
    <dbReference type="eggNOG" id="arCOG01872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_2_1_2"/>
    <dbReference type="OrthoDB" id="28434at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF81">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="8">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="195" mass="22559" checksum="A752C71FC062F82C" modified="2011-06-28" version="1">MKEEKKSSYKDFFDAIILAGGESKRFGTDKCSFEIDGKTMLQRVAENFENPIIVTRTPRNCRIEIIDDGKGPIKALEIAIKKIEKDKVFVTGCDFPFIKRKVVEYICSKDYEITMPLLEYPQPLLGCYDTRFLKSNISRIVSLQDLIYLANNVYLIGTEEIKMIDSSLDSLRNINRLTDFYTKIKIFTKSKILLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P6</accession>
    <name>F4B6P6_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775372.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6P6"/>
    <dbReference type="STRING" id="933801.Ahos_0568"/>
    <dbReference type="GeneID" id="10600012"/>
    <dbReference type="KEGG" id="aho:Ahos_0568"/>
    <dbReference type="eggNOG" id="arCOG05986">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424309_0_0_2"/>
    <dbReference type="OrthoDB" id="42571at2157"/>
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    </dbReference>
    <dbReference type="NCBIfam" id="NF046072">
        <property type="entry name" value="UpsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="645" mass="75021" checksum="41F48A2A708D15DB" modified="2011-06-28" version="1">MKIPNYKYISRIFIPEGVNYTLENIENTIYLKTDSIRQPEIILFPEVNIGKEPWVVQKGNKLIQIDGKEEVLEESHGDFLGVTYISVEGVIEKVLQIREDEFIFKGNKYNKFLKFFNTYILENNRKITFIYDNMEIDREKPKYYIVNKNGISLVYDGYTEIISKSGSLTINEEKILLGRYGNRSIFQGIDGKILLDEDVIGFCENKAELLGVINNGIVISCGDKVKYFSDGIWQEIEINANPFLSFVNSNFIILTADSYTLVYDKNLFLIYKFLSSGASATTRYIVLFRNPFVGVVDSLDDEDVIRTEKNVLDTSSPIKILIRKNYDIEYQNVIEVNRRDINSKYYEVEVEPKLLGDYEAKFKLISPFFSLNANIPIISEKPRIYAKGSIIRTNGEVLGTKMNSYLNLTINTKIITNIPYVIRIRFRDKLFDFTLNEKEVNKELKIPINIFDKKDSNEIIKIEIIRNNVIQSSMEFLVPIIFVEPPKEWKSKIVDLGDYIEKISYARKGDISWTKICHYPKHKKAILIKENSKEGIKISGDKVIVNLENPIENLYYTINYPYLVLIPKMKYYYPIEVFYGTHSYRGLPEKIIFPLDPAYDEVFIRIYIGDKTVKRTYKIPKEVYFKVAVNSKNKLEELLKSFGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P7</accession>
    <name>F4B6P7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775373.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6P7"/>
    <dbReference type="STRING" id="933801.Ahos_0569"/>
    <dbReference type="GeneID" id="10600013"/>
    <dbReference type="KEGG" id="aho:Ahos_0569"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_724819_0_0_2"/>
    <dbReference type="OrthoDB" id="377022at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="381" mass="43904" checksum="CCB0841095CB932C" modified="2011-06-28" version="1">MGCKVSISQVDNILNYTVEEPTLTAEEEKALKSQVQLPQNSIEKINYYKEKMEKFGKLTCLMLDKNVEEIECSDVSKPLAVKIKGLGRAITNIKISRDETLNIFSKFIDSKNYKRLGKYIVIENDKFKAIIKVKDDINFNIIKSDSLYSESITELISEGYLNKYSTAYLWHIIENSGIIIINGNKRIARCLAYSLINLVYNKFNKIIVVGKSKTRKLDDLIVLNDVKNLDIIDYYNPDIVLFDYIDNDVIVKTLDLNTQNKGIIILSEFPDNITFLSSLSKSQLLSLSKLPTILVEIVRNYINIHELYSIRTKVRIRKVCKKGNMNKKEFSYSITLKNKINTNNLDLKVSIIENLTNNNILDKYEIREKVMKIGEKIEPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S0</accession>
    <name>F4B6S0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Allantoin permease family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1736</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776528.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S0"/>
    <dbReference type="STRING" id="933801.Ahos_1736"/>
    <dbReference type="GeneID" id="10601236"/>
    <dbReference type="KEGG" id="aho:Ahos_1736"/>
    <dbReference type="eggNOG" id="arCOG03447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026016_3_0_2"/>
    <dbReference type="OrthoDB" id="27121at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF1">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="391" mass="42888" checksum="4D664292FF9C6C6D" modified="2011-06-28" version="1">MSVKPRSILTLWFSSNTAVAKYAIGLIPATLGLSPTLTIISLTLGNFLASIILGIATSMGPRFNKEQIRISQDVFNNGWKAFSFLNFLNTLGWFIVNVTLGGFALSVLTNNVALGGLIIVVIDVSVVIFGSSFIHKFESYVSLLLVALGIFISAEILTVPMKIPEGGSFLSFWVVFLTSFGAILSWSPYASDYSKGLNLDFKKSLFYTSLGVTVPSVWLSYLGYLSALAIGKSSPIADVLGVLGKYFLAGVVIMVLGIVSADALNLYTNTVALTSIVNIKREIATIIAGVLGFIAFYFIYKNFLDFLINFLSSLGYWISPWIGVLIAYLIKRKDWKRAWVSFSLSVIIALPFMNLRQYGIPYEGFISSMMGGIDVSQIVMFFVSILFYLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6V7</accession>
    <name>F4B6V7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved pro-fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776565.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6V7"/>
    <dbReference type="STRING" id="933801.Ahos_1774"/>
    <dbReference type="GeneID" id="32173778"/>
    <dbReference type="KEGG" id="aho:Ahos_1774"/>
    <dbReference type="eggNOG" id="arCOG08408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346348_0_0_2"/>
    <dbReference type="OrthoDB" id="382485at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="203" mass="22544" checksum="A7AAAAB32C60DF73" modified="2011-06-28" version="1">MRKSLSLLGLGLLILFLLEIPISAIHAGATVLPLGTDPNTWVIEFYPCSYPSGVSPSNYSVTVNFTIPGFTHTSGCYLAFVLTTWVEASEYTGIVYSGTVYLALQLGVVFVYSSNTYVINEQVWTCNGNEIYHNYTLVTTTIHPNVETLITLLYRYDNGYVATGILQECTSPITLIDVHYPTSYRYGCTIFYVINTYGFTDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X0</accession>
    <name>F4B6X0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mannonate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1787</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the dehydration of D-mannonate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:20097"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17767"/>
            <dbReference type="ChEBI" id="CHEBI:57990"/>
            <dbReference type="EC" id="4.2.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; pentose and glucuronate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mannonate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.8" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776578.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X0"/>
    <dbReference type="STRING" id="933801.Ahos_1787"/>
    <dbReference type="GeneID" id="25395614"/>
    <dbReference type="KEGG" id="aho:Ahos_1787"/>
    <dbReference type="eggNOG" id="arCOG05324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091165_0_0_2"/>
    <dbReference type="OrthoDB" id="39900at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008927">
        <property type="term" value="F:mannonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042840">
        <property type="term" value="P:D-glucuronate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004628">
        <property type="entry name" value="Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30387">
        <property type="entry name" value="MANNONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30387:SF2">
        <property type="entry name" value="MANNONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03786">
        <property type="entry name" value="UxuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="13471" checksum="F2F786BD3AEE956A" modified="2011-06-28" version="1">MNEIPRIRIAEILLDNSPTLWKNLEEFLKFILPIAEDAGVKLAIHPDDPPIDEVMRVARIMNNVEAFERLINEFPSEYNGITFDHSLFSLMTDDLVSVVRHFLEKKRIFSFTLGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B700</accession>
    <name>F4B700_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphoglucomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0595</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775399.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B700"/>
    <dbReference type="STRING" id="933801.Ahos_0595"/>
    <dbReference type="GeneID" id="10600042"/>
    <dbReference type="KEGG" id="aho:Ahos_0595"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000001">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
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    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="154"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="259"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="384"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="454" mass="50069" checksum="DD7D32B840A9F7BA" modified="2011-06-28" version="1">MGKLFGTDGVRGTINKELTIELAQKLGQAIGTFFGKGSTILIGRDARAGGDMYTRAVESGLLSAGVKVYEGGFAPTPALQYAVKTLGYDAGVIITASHNPAEYNGIKVIDKDGVEISRDKENEIEDIYFSGKFYSEDWKALTYDVKKEDRVIDNYVKGILSHVDVEKIRKKNFKVLIDGANSVGSITSPIVARELGCKIYGINTNLDPLFPARTPEPTFDSLKDTAEIAKGLKVDLGVAHDGDADRAIFIDSQGRVQWGDRSGALLSYWASTKESSFPRRIFTAVSSSVLVQDYLKKYNIEVVWTKVGSVDIAHKLMEEKGIAGFEENGGFIFPLHQYVRDGAMSFALMLEMLASVNISSAELFDTLPKYYTVKTKVYINKNTDVEKIYETVKSTYSHGNIITIDGVKIITPDYWFLVRKSGTEPIIRVMVEAKDKEKAENLANELVKLVGSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B707</accession>
    <name>F4B707_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Archaeal Sm1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B707"/>
    <dbReference type="STRING" id="933801.Ahos_0602"/>
    <dbReference type="GeneID" id="70682497"/>
    <dbReference type="KEGG" id="aho:Ahos_0602"/>
    <dbReference type="eggNOG" id="arCOG00998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076902_11_0_2"/>
    <dbReference type="OrthoDB" id="371816at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="87" mass="9770" checksum="5F4A0931ED11B537" modified="2011-06-28" version="1">MQAKVENPLKNIKSAVNKIVLVKLKDGSEYIGKLEQTDGTMNLVLRDCTELREGTAEPVAKYGRVLIRGSNVLFISIDYETIMNKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B747</accession>
    <name>F4B747_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetate kinase superfamily-DUF1464</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775446.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B747"/>
    <dbReference type="STRING" id="933801.Ahos_0643"/>
    <dbReference type="GeneID" id="10600092"/>
    <dbReference type="KEGG" id="aho:Ahos_0643"/>
    <dbReference type="eggNOG" id="arCOG04329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075234_0_0_2"/>
    <dbReference type="OrthoDB" id="146520at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:0016301">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009927">
        <property type="entry name" value="DUF1464"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07318">
        <property type="entry name" value="DUF1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009433">
        <property type="entry name" value="DUF1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="299" mass="33307" checksum="824524D33AE02AD5" modified="2011-06-28" version="1">MLFAGVDPGSESYAVTFVDEIGRIVEYYEIPTGLIIHDSMRLVKLIRDKRPNTIALPSGHGLPFVRASRIGEKEIFLLTLADPQKEGPLRSFLRAIIPEENAFTIPSVIELESVPEYRKINKIDMGTADKVASAFFYRTIFDSFVLIEMGRHFSSVIVVKDGKIIDGFGGTEIPGLVSPGCIDGEIAYLLCKYSRITKDTIYTQGEEKRSLEILRIVSEWYSEKLDLPIIASGKAKDKIDFGIKFEIKYKEASVGAAFIANAIGGGIFRKYIDMLNSSGTALDYVRLKGWEEITSLIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B749</accession>
    <name>F4B749_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Triphosphoribosyl-dephospho-CoA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775448.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B749"/>
    <dbReference type="STRING" id="933801.Ahos_0645"/>
    <dbReference type="GeneID" id="10600094"/>
    <dbReference type="KEGG" id="aho:Ahos_0645"/>
    <dbReference type="eggNOG" id="arCOG04238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063627_0_0_2"/>
    <dbReference type="OrthoDB" id="85890at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4200.10">
        <property type="entry name" value="Triphosphoribosyl-dephospho-CoA protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280:SF1">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="300" mass="34483" checksum="674ABF4F898995A0" modified="2011-06-28" version="1">MEDILDQLCNEVAYILSSSSIIEASVFKPGNASRIQDIKSVKYEDVLLSANLANAYYRKACIRGYNSSRPLYDLLYLTIKESKKMEVNYSILGTQLLLLPISYSSLLADNVDNLRRKLSELIISLDVEDTKWFFEALKQVELSYLGKIDVMDYRETANKTMYEVLVFSSNMDTIARNMIRNYEYSYIAYKIIKDSESLEKGIQKAFIEILSEQPDGLIYRKYGGKIALLISHLAREVKGDFTEKKLLDFNNFLVKNNYNPGSTADIIASGVALYLLDKWYEKTRNDYPLPLPRGCSRIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B759</accession>
    <name>F4B759_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MEMO1 family protein Ahos_0655</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775458.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B759"/>
    <dbReference type="STRING" id="933801.Ahos_0655"/>
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    <dbReference type="KEGG" id="aho:Ahos_0655"/>
    <dbReference type="eggNOG" id="arCOG01728">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="OrthoDB" id="372162at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="284" mass="32136" checksum="BE259B91E4BFE970" modified="2011-06-28" version="1">MKRLPAVAGAFYEADPQELKDRIVWSFTHPVGPGKLPTVPKEKGKRDNLFFIVPHAGYIYSGPVAAHSYYYLASEGKPDLVIILGPNHTGYGSYVSLWNKGCWETPLGDVKIDEEMAMELVKYSEVIDIDEQAHLYEHSVEVQIPFLQFFFDSEIKIIPIVILYQTPEIAEYIAEGIYRLMQKHPEKDIVVLASSDMNHYDPYDITYKKDEMAIEKIEQLDYKGLYDVVENKDVTMCGYAPVMVAMILAKKFNKKPYILKHATSGDTSGDKSSVVGYLAARFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B764</accession>
    <name>F4B764_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl18e</name>
        <name evidence="6" type="ordered locus">Ahos_0660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775463.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B764"/>
    <dbReference type="STRING" id="933801.Ahos_0660"/>
    <dbReference type="GeneID" id="10600111"/>
    <dbReference type="KEGG" id="aho:Ahos_0660"/>
    <dbReference type="eggNOG" id="arCOG00780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="OrthoDB" id="11309at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_eL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_eL18_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003079">
        <property type="entry name" value="PRK04005.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="115" mass="13147" checksum="0C13A79D1B6FFED8" modified="2011-06-28" version="1">MTGSTNIMIRKLIRSLEKQKKPLWKTVAEKLQTPSRKRWYINLYKIEKYTKPGDIVIVPGKVLGIGNLTHEVTVVALDFSKSAKEKILKAGGKVMSLYDFDKLDLKGKNVRLMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B767</accession>
    <name>F4B767_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps4</name>
        <name evidence="12" type="ordered locus">Ahos_0663</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="6">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775466.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B767"/>
    <dbReference type="STRING" id="933801.Ahos_0663"/>
    <dbReference type="GeneID" id="10600114"/>
    <dbReference type="KEGG" id="aho:Ahos_0663"/>
    <dbReference type="eggNOG" id="arCOG04239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="OrthoDB" id="10429at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_uS4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_uS4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="10">
        <location>
            <begin position="104"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="187" mass="21395" checksum="140D8B2FC144B026" modified="2011-06-28" version="1">MGDPRKSRKKWEGPGLPWIRELLLHEQELLGKYGLRNKKEIWLARTIVRKFRHMARGLLALPPAERMVREKQLISKLYKMGLLSSQNATVDDILGLTEEQLLERRLQTIVFKKGLAKTIYQARQLIVHGHIAIGGRRVTSPGYIVPKDEEDLIDYYPTSVFKTRPPTAQVTQSSQVTQGGEQNVQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B771</accession>
    <name>F4B771_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776606.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B771"/>
    <dbReference type="STRING" id="933801.Ahos_1816"/>
    <dbReference type="GeneID" id="10601316"/>
    <dbReference type="KEGG" id="aho:Ahos_1816"/>
    <dbReference type="eggNOG" id="arCOG00322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_1_2"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666:SF1">
        <property type="entry name" value="CONSERVED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43666">
        <property type="entry name" value="TLDD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="1">
        <location>
            <begin position="189"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="408" mass="46049" checksum="7EC70E0633C3134F" modified="2011-06-28" version="1">MQGFDEHAIFKTKKEVTVIKFVNGKVSTIQRLDNEVSYFLLKKGNKYIIMEGKELDPNFANVIDVMEEPMLSPKISENTGEYKFVKLDDKIEKIRENPEDVISLVLNSRYPISGIVNITRSTISLLTSKGFSGSDVRNSIDGYFRAFNGEFTGQWAFASSSYSESTIKESVNIACELASITKKAKVDDGVYNVVLSPLVFGNLMNYLAHMSSGLSIMMENSIFSRYKPEDRIASEKFTFSDVPKTDMPNAVEFDMEATFTKNKKIIDHGVFKTPLLNNEVAEAMNLKSTGNAGWINPIPWTLEVDEGDVGKDSLLSGNVIFFNNNWYTRFQNYVEGNFSTVGRDAVIVYKNGNVEGVAGRLRIADSLINIIRNIEEISKERYLVKWWDSRIPVLSPYVLVRDVKITRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B2</accession>
    <name>F4B7B2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776647.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7B2"/>
    <dbReference type="STRING" id="933801.Ahos_1859"/>
    <dbReference type="GeneID" id="10601358"/>
    <dbReference type="KEGG" id="aho:Ahos_1859"/>
    <dbReference type="eggNOG" id="arCOG03854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_333067_0_0_2"/>
    <dbReference type="OrthoDB" id="37011at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="858" mass="95841" checksum="ABDE440A5ED963B4" modified="2011-06-28" version="1">MNNALVTFLFLIITISMALGILVYYSTSSSIIEAKTNPLYVAEDYSKGLEVSSTQPVINYIIPQHKCQPINFTVNFILTVNIPGYQGGKLYLIPFLMPSNYNPYDYVPTSIPNQVSNPNYVGYVSIKIGDTQLKPIIFNNDVYFLNQQQGKIEAYAFCVCNGQTLNVTFVTSLNETPVIWIIANICGQMYRLAYPYFISLSKGINTANGLITSPPLTYYELYQKELGCLAILFSSSSCSHVVFYGNDKIKTNHGNIGYFAKKGYLCLDSDIKFCCVCELYLGKCIKVCPPSAHKYLPKCCRLEYVPPCPCVFINPIYQYYNPSNCQEELQSKINNVQFCKCTTTVSCMINNHFIKYCNGVLYVCKPIRFCEPLSIERYIWINKQTQCPVKCIIFKCPVIFDNGFIDKSDVNLKFCKCIVSEGLTCVGGIACLTFEKGALFICTTNKNSGELILSGTNTEQECGIHIISCGPFIAYFECAPGSIVKLNGVHLQDKNNIISFTAKNICIFGVDKICSCYPLFIQAPAHNTINFCHVTICGGLVLESDCHSTLTFEANNELCIKKGPLIIDTGSCSYLTMDGRSEIHVCGYALIKANTITLHENAKIVTSKYDLFYGCRISFCGDTIVVCNNQPPQGKLSQIVIYPTITSSFGVAGWFSIFPNDTEVNYLNIKLSNNCCTFCMFLNISPLNNGKYNITPIIHPSSILSSSSGAKPLRFTKPTCLIVEQGAKIFYNIYVSFSGCHVGISIYLYTQCSKEYHCSTCTWCCGTSRGYIGVPKDSHLSVSLYGEPTYQVLLYEETQPQAVYLMEYCGPYVKLNLNCETPVEYFYLISSCINDNILTPTYSNINNGYCIYHVTYIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C7</accession>
    <name>F4B7C7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sulfocyanin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1874</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776662.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C7"/>
    <dbReference type="STRING" id="933801.Ahos_1874"/>
    <dbReference type="GeneID" id="10601373"/>
    <dbReference type="KEGG" id="aho:Ahos_1874"/>
    <dbReference type="eggNOG" id="arCOG03700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105306_1_0_2"/>
    <dbReference type="OrthoDB" id="56344at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04230">
        <property type="entry name" value="Sulfocyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049544">
        <property type="entry name" value="SoxE-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034246">
        <property type="entry name" value="Sulfocyanin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06525">
        <property type="entry name" value="SoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfocyanin-like C-terminal" evidence="3">
        <location>
            <begin position="61"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="215" mass="23611" checksum="4D87913F2C6FB0EC" modified="2011-06-28" version="1">MRALSKIGAVILILIFGGTAIMAGFLAYNFAKVTYPVHCYQPTVTTTTPVTTTVPPTQITHNLLPYNPSTKTVYLYISTWDNTTLYNFNGTSDGELHIFIPAGWKVCVIYTNFEPIDHNFIIVKNTTAVPSCSIIPSSNIILYVGVSSSNYFDSGLSSGASATGSIVLSPGYYWFACGYEDHAESGMWGVIECSSSITQPYYEVTNEEIEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G2</accession>
    <name>F4B7G2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Selenocysteine-specific elongation factor SelB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775483.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G2"/>
    <dbReference type="STRING" id="933801.Ahos_0680"/>
    <dbReference type="GeneID" id="10600131"/>
    <dbReference type="KEGG" id="aho:Ahos_0680"/>
    <dbReference type="eggNOG" id="arCOG01564">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_913990_0_0_2"/>
    <dbReference type="OrthoDB" id="30874at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="298" mass="33328" checksum="28E7618982D664DA" modified="2011-06-28" version="1">MYYGNIISVISSDKEKAGKIAESLGKLHENEKLRIYYRKKGDYIRSILVPTEYPEKILQAAEAVSLSSYCVLYLPIVPSWTEGELALLITSAGCKGEIITEMPESDVRKLFKGLPLEEFPIHQTPEEFEGKEEDKGVVYIDKAFVVKGVGVVITGFSYTQVKVHDKLKAIPQNKEVEIKSIQVLDEDQEAVDTGIRIGFALRNVREDEMKDSLLLVKNEVKTVKSFSGEIFLFPWTKIESGNYHLVANGVSVMANLKINGSNVDVELSNEMPLSKRFIVVNVNAKQGKPRIAGYVIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G4</accession>
    <name>F4B7G4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775485.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G4"/>
    <dbReference type="STRING" id="933801.Ahos_0682"/>
    <dbReference type="GeneID" id="10600134"/>
    <dbReference type="KEGG" id="aho:Ahos_0682"/>
    <dbReference type="eggNOG" id="arCOG04223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_6_1_2"/>
    <dbReference type="OrthoDB" id="11182at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002096">
        <property type="entry name" value="PRK00939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01158">
        <property type="entry name" value="SUI1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="11079" checksum="6EFF35D8207E5675" modified="2011-06-28" version="1">MADNLCGGLPPDICEQLNKEEQFIKIKLEKRRYGKEVTVIEGISSDDADLKKIASELKSKLAAGGTVKNGRIEIQGDHREKVKDILVKLGFPAENIMVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H9</accession>
    <name>F4B7H9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="10">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0697</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10 11">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H9"/>
    <dbReference type="STRING" id="933801.Ahos_0697"/>
    <dbReference type="KEGG" id="aho:Ahos_0697"/>
    <dbReference type="eggNOG" id="arCOG04184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_2"/>
    <dbReference type="OrthoDB" id="372108at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000001">
        <property type="entry name" value="dITP/XTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011396">
        <property type="entry name" value="PRK14821.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ham1p_like"/>
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    </dbReference>
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        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <feature type="binding site" evidence="10">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
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        <location>
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        </location>
        <ligand>
            <name>substrate</name>
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    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="171"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="186" mass="21477" checksum="54B29AB5AAAA72D0" modified="2011-06-28" version="1">MKKEEVKVVTGNKRKFEEMNQIALQYGIKLSMINLPKFEIQADRLEDVVRHAASVFYSILNEPIILEDSGLFIEALNGFPGPYTKFVKKTLDINGILKLMKGEKNRNAYFKTALAYVNENEIRIFTGEVYGKIAEEARGNKGFGFDPIFIPEGSEKTFAEMEIEEKNKYSHRSKAFKKFLDYYIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J4</accession>
    <name>F4B7J4_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">PIN domain protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_0712</name>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107892_0_0_2"/>
    <dbReference type="OrthoDB" id="35744at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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        <property type="entry name" value="PIN_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="16409" checksum="ABABA654729C6B3D" modified="2011-06-28" version="1">MGANKNLGILVDTNILLYVYDRVDPFNLVIEYLDYKPVFYIHKAVFHELEILRSRYSRSSSYMSRINIALIYLEKYKNYWKLIGDEIDKPTDDILIDCAKRNKLLIFTNDKELKQKALKNNVGIIFLASKGKIIKSLFPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q5</accession>
    <name>F4B7Q5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pseudomonapepsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1929</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="STRING" id="933801.Ahos_1929"/>
    <dbReference type="GeneID" id="10601428"/>
    <dbReference type="KEGG" id="aho:Ahos_1929"/>
    <dbReference type="eggNOG" id="arCOG03665">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006754_0_0_2"/>
    <dbReference type="OrthoDB" id="56693at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017001">
        <property type="entry name" value="Pept_S53_physarolisin-II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17957">
        <property type="entry name" value="Big_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032623">
        <property type="entry name" value="Peptidase_SSO2181_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1260"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="9">
        <location>
            <begin position="197"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1286" mass="138990" checksum="23B55B48BD27FB1B" modified="2011-06-28" version="1">MRGKSIIILSLLIFATIQFLPIIHSQPTAPPGFKQAGNLQPNKEVIFTVYIPLKCTCKIFYYAEETSNPSSPLYHHFLTKSQIQQFLPTEKYEEVLSQLKADGFKIILSTLDSVIVAEGTVSQIHHYLGLNFAVYTNGTLTFYESYGTPTIPGVAIVSQNLTADLFAHPQFLITQKDIQSLASKLTDQANITEPIEAYWPTALQKVYNATALYAMNDYGQGYNIGILDFCGDPYIFQQLASFDKVTGLPSTNFTVIPIGPYDPSKGIITGWAGEISLDVEVAHSMAPKANITLYIANGALPLSTVIACIVNQDIVDDLSQSFGISESIFSLFNAQEFYTCVYETDVYYALGSAEGITFLASSGDGGGSGYSNGPIGSVIYPSSSPFVTSVGGTTTYIQFPGNSTQTAWSNYGFVPDDVNFGGSTGGVSVIEPKPYYEWGIPTPSTYPAGKTTPAISANANVYPGVYIIFPGNVTGITGGTSEASPLTAGLLALIMNYAHTKLGNLNPVIYMFGENSTIYPKVFNPITFGYNIPWTATYGYNLVTGFGTLNVGEFALYYNMTMKEKKELNIQVQVVNSSGESQPQYFPGEVIEIIANVTYNDTPVSSGTFTAIISNVMGNISRVIMAYNPLTREWFGIVKLPSNANGILSVTVIGESNGIIGYGMDEIFAGYYAQFLLPETFVPILANECIIVANVTNALGQLYNFSTVNITIYKYCILNNSYTFVSNVTLSITPSGVAWVGNYPSLSPGDYLIIADNVFGFVAFTNGVSLQGEYPYGLFILPPVIEEPGSVYPGENIIIQGIIQPPSILFEYISSSLGISEYSAVMDGSNVTAELVNPEGKVVSTAKIFLNCQGIYFGELHVPSDSTPGLYTILLHSSYFSLSLGQYINGSFYGQIYVQEPSTVNVKVQSYSFEGQTLTVYANITYPNGTEVKYGMYSATVYPQSLCFEYSQISIIVELPLWYNAKLGLWVGNVTLPSTLSPGNLTYLAGLNYYGAPFRILITGESASGYSTSPSPSLAGITYVLPYTAIEGKTIDGFQTYDSALINDTIITNGTLTNDVLINDTIIGHVTIVGSNLTNVKFLNSSIALISSSGSNLYLDDSNITLVDSSVDGITLVNSMITIESSKISNISPALPEIKIISPTANQNLTGVYTISFNISGEDISKVLVLLNGQEITEFSGNGTFSYALNTTKYPDGSYTIEVEAIQADGLYTRSSVTVNFENQLKNLYTNVKANETSLSSSINNLDNSLTSVKTSLSTLIYITLGLAVIAIIIGIVAIIISRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S2</accession>
    <name>F4B7S2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA+ ATPase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776734.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S2"/>
    <dbReference type="GeneID" id="10601445"/>
    <dbReference type="KEGG" id="aho:Ahos_1946"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_1_1_2"/>
    <dbReference type="OrthoDB" id="371918at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF19">
        <property type="entry name" value="ARCHAEAL ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="44"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="230"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="432" mass="50229" checksum="64EE4F97BA7DE1C7" modified="2011-06-28" version="1">MDREKILKALIDWNFWYKEQFTGYPRNYSKQVLDIMKSGFVADVIGVKRSGKSTIFNQVISHLIKEEKISPFLTLLINFEDSRFAEIDNADKLFSLLHIYREETGINGKPYIFLDEVQKVKGWEGFVRSLIDRKEAYVAVSGSTSIINKGKVREVLAGRHISVEVLPLSFSEYLEFKGVQIGREMDLLAREEEIKKLFLEFLKFGGFPAVVNSKIKDKILLNLYDDIITKDVITQCKIRQEVKLRELSLFYVSNVGNRISFRKISRSLNIPLSTVERFTSCLLDSMLIYFVPPLSPSLKEMVKGEKKVYSVDQGLSNVVGYKLNESLGSLLENAVYLELRRRYGGDSIFYYHGKNEVDFIIKINNEVRYAYQVTFTLNDEREIKGLKELEKKAGKKRVEKFIITFDDEEKEIDEIRVMKAWKWMIHFISESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T6</accession>
    <name>F4B7T6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T6"/>
    <dbReference type="KEGG" id="aho:Ahos_1960"/>
    <dbReference type="eggNOG" id="arCOG03849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048388_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="398" mass="45054" checksum="10B9AC5CFF84BDEE" modified="2011-06-28" version="1">MMIAVSPFNTRFGLASKSDSDFLALLPFDDDKIIASLILGSFIVNVIFLSIFMYWFIEALGIAGATIIPAFALLSSSLPAVLYNLNNRRKILIDLVIVTWFISAIFNFQLSPISMFYGYFEGYVISYAFAFVTFSIALKKFNFSSYSNFTVSSEKGEVKEEISFKSSNPLVLTLTRNLRILEVGGRVNWMGVSTFVSRRVELWKVMLGSSISAIILYFILSRINDGSFLVFYVLFGGWFFMIMIASAAFITEPIWLDMNVMTPEEFSRYYLTSKALSIAIILSPFAILSLILGYLGDALGFIVFIPLSSIFIMSLYARYYQQRYNMPINVSPIRLLVSLLTSIPLIPLIILSFLPAYLILCLPIMFSFIVFYLTISLPFIFSKSYWESTIEKIVTSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U0</accession>
    <name>F4B7U0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoF synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U0"/>
    <dbReference type="STRING" id="933801.Ahos_1964"/>
    <dbReference type="KEGG" id="aho:Ahos_1964"/>
    <dbReference type="eggNOG" id="arCOG01870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128032_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052209">
        <property type="entry name" value="CbiZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336:SF5">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15607" checksum="183DD81D16CD2C25" modified="2011-06-28" version="1">MDKNYCKNAFEDVEKLREGNTIIFITAAKNYEFKQTSWGELFISAGVGESGENAGCTINISAFVNYPLNLNGLVDLVRSLTEAKSGALKDLNFPFTGTASDAIAVGTIGGNEYFAGPSSEIGKKVTKDVREVLRKLLIRDLSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y1</accession>
    <name>F4B7Y1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">radA</name>
        <name evidence="14" type="ordered locus">Ahos_0772</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9 11">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 11">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y1"/>
    <dbReference type="STRING" id="933801.Ahos_0772"/>
    <dbReference type="KEGG" id="aho:Ahos_0772"/>
    <dbReference type="eggNOG" id="arCOG00415">
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
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    </dbReference>
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        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF14520">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
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        <property type="entry name" value="AAA"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rad51 N-terminal domain-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="12">
        <location>
            <begin position="66"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="13">
        <location>
            <begin position="243"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE93658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="305" mass="33540" checksum="CF3B0D798BB94961" modified="2011-06-28" version="1">MGQAVLNKLIDAGYSSLEAIAVASPQDLSVAAGIPLTTAQRIIKEARDALDIRFKTALEVKKERMNVRKITTSSQALDGLLGGGIETRTMTEFFGEFGSGKTQICHQISVNVQLPPEKGGLSGKAVYIDTEGTFRWERIEAMAKAAGLDPDTAMDNIYYMRAINSDHQIAIGDDLQEFIAKNPSVKVVIVDSVTSHFRAEYTGRENLAARQQKLNKHLHQLTRLAEIYDLAVIITNQVMARPDMFYGDPTVAVGGHTLYHVPGIRVQLKKSRGNKRIARIVDAPHLPEGEIVFAITEEGIRDAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B814</accession>
    <name>F4B814_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PUA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="10600266"/>
    <dbReference type="KEGG" id="aho:Ahos_0805"/>
    <dbReference type="eggNOG" id="arCOG00991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116577_1_0_2"/>
    <dbReference type="OrthoDB" id="7576at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PUA_archaeosine_TGT"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PUA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TGT_C2"/>
    </dbReference>
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        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
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        <property type="entry name" value="unchar_dom_2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="92"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="19713" checksum="932CDE1C259C02A8" modified="2011-06-28" version="1">MKEFVTYPEKVSEFDFFYLKNIFAYQFGYDIADCVFDNSNSFFIQRSINTNRIRNILIDNNLFLVLRAQDNLFSLTLDSAKKIKMCSKPPRFRVIIPYDIAKVVRYSGNVFAKHVIEVDKSLRAGDQVIVVDSDDNIVAVGRLKLSAEEIIEYKRGVAVIVKERNKNEGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B834</accession>
    <name>F4B834_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776773.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B834"/>
    <dbReference type="STRING" id="933801.Ahos_1986"/>
    <dbReference type="GeneID" id="10601485"/>
    <dbReference type="KEGG" id="aho:Ahos_1986"/>
    <dbReference type="HOGENOM" id="CLU_1187727_0_0_2"/>
    <dbReference type="OrthoDB" id="373159at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="233" mass="26641" checksum="192257BBE0D88BAC" modified="2011-06-28" version="1">MIGRILVPSVSIILIGLGVIYFTLSSLYSSLCYAKIIQFNEFTYRYVKYNATEFLFNGSVFLCYSINADKSVYVEGILNCSFKEIVKICKLVYGCLRCFKRIPEIANITYLKILIGNYSEFKYAKPRIENATIKLKGEILNCNSILKYIVTNCGNVPIYLLKLYVHIVCKKCVKEGNKTVTKYVCKNYSINVYNWLNPGKIYKYCTLICKLACAAITLCYKALCNTMYCTVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B880</accession>
    <name>F4B880_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776819.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B880"/>
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    <dbReference type="eggNOG" id="arCOG07184">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299183_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="11294" checksum="9173FFFC8D4FD52E" modified="2011-06-28" version="1">MFANYTPLKIDPENKIYVAKVNTTGQIIEIRDVDLSRMMGHVRLDHPGATVEEVVNMDLAEVVKQTGDFKDDDERGDYAMTLYLGLAYAIKYGLLILVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B885</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776824.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B885"/>
    <dbReference type="STRING" id="933801.Ahos_2038"/>
    <dbReference type="GeneID" id="10601538"/>
    <dbReference type="KEGG" id="aho:Ahos_2038"/>
    <dbReference type="eggNOG" id="arCOG10266">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192573_0_0_2"/>
    <dbReference type="OrthoDB" id="372183at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="27022" checksum="15ADC14ED86F14E2" modified="2011-06-28" version="1">MLKKKIIRAIDDFFQYKKSGDDEILDEVLDKIEYKDKYVIIDGEEKIKVDKKKDVVGVFLANIPYIILGPGELHDDLPEKVRKIQSGAIKLLEIGGFNEIATLEIYLVMEMSLRALYSEWLKKAEYVKYGKKKVKVKNLDYRKLKLLLKRKGWSKYKVKVNGEAFPYSQGSLLAWAERFMDDKTSLAFRLAINVRNLLAHGEFEWNLYPTMRSLESASQASWLLFEKLRRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A6</accession>
    <name>F4B8A6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0819</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775621.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A6"/>
    <dbReference type="STRING" id="933801.Ahos_0819"/>
    <dbReference type="GeneID" id="10600286"/>
    <dbReference type="KEGG" id="aho:Ahos_0819"/>
    <dbReference type="eggNOG" id="arCOG00109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="OrthoDB" id="27149at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011528">
        <property type="entry name" value="PRK14968.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="4">
        <location>
            <begin position="27"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="197" mass="22132" checksum="0E3F79F0D5F53A01" modified="2011-06-28" version="1">MSSYRTIEFNGIKLCINDEVYEPAEDTELLLSIVKIKKGEKVVEIGSGSGILSIISAKMGGKVYSIDINPFASLATLCSSKLNNLYIDVINCDMLTCLRNITFDVGIFNPPYLPFEEYDDWIKYSWSGGKSGSEVLVNFINIISAKRLYILYSSLSNEDEILEAIRKRKMVINRKIEKTFGFERLIALELNAEGSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B0</accession>
    <name>F4B8B0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0823</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775625.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B0"/>
    <dbReference type="STRING" id="933801.Ahos_0823"/>
    <dbReference type="GeneID" id="10600290"/>
    <dbReference type="KEGG" id="aho:Ahos_0823"/>
    <dbReference type="eggNOG" id="arCOG00357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="OrthoDB" id="5875at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01669">
        <property type="entry name" value="TGS_MJ1332_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000002">
        <property type="entry name" value="GTP pyrophosphokinase (RelA/SpoT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
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        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="2"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="401" mass="44822" checksum="AFAFF49EBDA5DC26" modified="2011-06-28" version="1">MITVGLIGKTNVGKSTFFSAATLVDVEIANRPFVTIEPNVGMAYVKTLCVHNEFKVKCNPRNSICIGDYRFIPIKLVDVAGLIPGAHEGRGLGNKFLDDLRKADVLIHVIDASGSTNEEGVPVEPGSRDPEEDIKFVEDEINEWFISIIKKDWEKFARITDLGNKDPIDALLSKLSGLSINREQIIQSLKESKLENVKFMQWSEEDIRRFGITLRQISKPIVIAANKDDIPIARKNIDRLKEKYKFLIPTSAEAELALRKAAKNGLIDYIPGEKEFKIRSNNLPEKQRKALDYIKTNVLDVYGNTGVQQAINEAVFGALNMITVFPVEDERKLTDRNGNVLPDAILIKRGSTPKDLAAVIHSDLAKGFLYAIDARKKIRVGENYQLQDRDVIKIVSSLAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E5</accession>
    <name>F4B8E5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps14</name>
        <name evidence="8" type="ordered locus">Ahos_0858</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775660.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E5"/>
    <dbReference type="STRING" id="933801.Ahos_0858"/>
    <dbReference type="GeneID" id="10600326"/>
    <dbReference type="KEGG" id="aho:Ahos_0858"/>
    <dbReference type="eggNOG" id="arCOG00782">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177289_2_2_2"/>
    <dbReference type="OrthoDB" id="5615at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_uS14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_uS14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="54" mass="6255" checksum="05ED521848198F5D" modified="2011-06-28" version="1">MGKYKPPAERKHGKGVQACRRCGSTDSVIQKYGIYLCRQCFREVAYELGFKKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F1</accession>
    <name>F4B8F1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps5</name>
        <name evidence="12" type="ordered locus">Ahos_0864</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775666.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F1"/>
    <dbReference type="STRING" id="933801.Ahos_0864"/>
    <dbReference type="GeneID" id="10600332"/>
    <dbReference type="KEGG" id="aho:Ahos_0864"/>
    <dbReference type="eggNOG" id="arCOG04087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="OrthoDB" id="38155at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_uS5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047866">
        <property type="entry name" value="Ribosomal_uS5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003125">
        <property type="entry name" value="PRK04044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="54"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="214" mass="23621" checksum="973A7CF5F0F5A066" modified="2011-06-28" version="1">MAEGVPTTNIEEWKPRTKLGQLVKEGKITSIKEIFEKNLVITEPEIIDALLPNLKYEVIDIKMVQKQTDAGELSRYKVLVIMGNFDGYVGIGMGKAKQLRVAIQKAIRDAKMNIIPVRRGCGSWECTCGEPHSLPFTVTGKAGSVEVTLKPAPKGTGLVVGSVLKILLSYAGIKDVWSSSKGETRTTENFIKAGYNALYNTYKFVTPADWARKR</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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            <strain>W1</strain>
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            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="100"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="17945" checksum="6278A2F9E5D35598" modified="2011-06-28" version="1">MEIYIFYIILLSFFSNFVVYLFYKYYISKKLNYIIIKLKEYDERLGKIVSDKRKEKVQKKIQNEIKSYNSTMYFYSFMQTALLIFIYFIDLSLVVFRMNFNLCLPYYIPLISIQYNGKYIIPYSSFIIFILSFVLFTPISLRRPKII</sequence>
</entry>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="entry name" value="CedA_arc"/>
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        <property type="match status" value="1"/>
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    </feature>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000313" key="2">
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            <dbReference type="EMBL" id="AEE93759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="262" mass="28124" checksum="7B1FDCAE6C46BA0E" modified="2011-06-28" version="1">MFNIGEMLFLASSLGALTYFIGTLIMGLPIPIYGIKKWGPKLILDGLYVTALVNAYGSILTLSSMIQSYLGATWCNFFKWVCCLIIQEIALNAFLRTIYGIIAVASGPTAGVLLSEVGVLFSLFGSLITTSETLLIIARIVYEYSQVFIALGILLISIPFRVGRGVGGSLIGFSIVFYAALPYLPNFLTSLGANVLQDLNNIPNNNACCIVVYLMTKVVPLLIEGTIVLPIAYMIILSGISIGVGAAISGYSSRLPIPIEIF</sequence>
</entry>
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            </authorList>
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            <strain evidence="1 2">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
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    <dbReference type="STRING" id="933801.Ahos_2062"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AEE94933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="169" mass="19193" checksum="7E6C7288A1A9CAB2" modified="2011-06-28" version="1">MYSLNFTREWDSALFEFTKSLKERLGNNLVMIIGLDENEKVYDSNVLIVVRSKTDDVIMSIADVALDVNSKYNCSINFYVCTEKDVEIIDAFSHSGKYDDCEKSFNEFKNRVLKISGVIDVQRTEGYDSNVLIVVRSKTDDVIMSIADVALDVNSKYNCSINFHVVQNG</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166563_0_0_2"/>
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        <location>
            <begin position="81"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="111" mass="12723" checksum="9AB2179870332B76" modified="2011-06-28" version="1">MKRILRIPRFAKDGKPKTLELLIDSASINDKGFAQEAKFLLVIDDGNNRVGFQLTTAEASLLYHRLGYALNEASKEYIEMEEKSKKNYEEKKGSKKSGEEEDFSDLMDEEE</sequence>
</entry>
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    <accession>F4B8Q8</accession>
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        <submittedName>
            <fullName evidence="1">SEC65, signal recognition particle 19 kDa protein</fullName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F4B8Q8"/>
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    <dbReference type="eggNOG" id="arCOG01217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
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        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="91" mass="10470" checksum="A94773341CE119B9" modified="2011-06-28" version="1">MSLRDYEGNKIAIWLAYFTADSRRKGRKTKKVKITLDDLFSAAKALGLEPEVLDKVHPGSRVKGLIMVKKVKGKYKLIKDIYSYLQQQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R0</accession>
    <name>F4B8R0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775696.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R0"/>
    <dbReference type="STRING" id="933801.Ahos_0894"/>
    <dbReference type="GeneID" id="10600366"/>
    <dbReference type="KEGG" id="aho:Ahos_0894"/>
    <dbReference type="eggNOG" id="arCOG00498">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061754_0_0_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF14">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="265" mass="30086" checksum="92CE559F7CC9E035" modified="2011-06-28" version="1">MKLSKNVEVIPGSPNTLIYDDRVVIDQGGKNSDLNINAEVQLATHGHMDHIAGLFKNAKIKFLPKEDYWSLNIIGRRAITYGFSAKNSSIFTYDLIKEELKDEFHDSEVEKIKLPGHTPGHVAYKIGNVLYCGDAFFGQKVLESFVFPFYTDFWAAQDSLDKLKELTKSADFIIISHGPIYKKEKMIEILNFNINYNKKLVEEIKDMIRGNPMTAEEVVIRLMIKRGKNEINPTSVILNEITAKSILSEVAKNIKVENKGVTFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S3</accession>
    <name>F4B8S3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S3"/>
    <dbReference type="STRING" id="933801.Ahos_0907"/>
    <dbReference type="KEGG" id="aho:Ahos_0907"/>
    <dbReference type="eggNOG" id="arCOG01905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186533_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9838" checksum="40373FC9CA135D2B" modified="2011-06-28" version="1">MTNLAEGELSIKELAEYTGIPKKNIIKAMKSLENRGYVEKRAIIGRDIIFSITEEGLEELYKYYLFMKKLIEDMEFTLCSRFDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V2</accession>
    <name>F4B8V2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V2"/>
    <dbReference type="STRING" id="933801.Ahos_0936"/>
    <dbReference type="KEGG" id="aho:Ahos_0936"/>
    <dbReference type="eggNOG" id="arCOG02200">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387511_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00322">
        <property type="entry name" value="FNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050415">
        <property type="entry name" value="MRET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47354">
        <property type="entry name" value="NADH OXIDOREDUCTASE HCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47354:SF5">
        <property type="entry name" value="PROTEIN RFBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="22271" checksum="BB1E4E57E8E809E8" modified="2011-06-28" version="1">MAMTKILSKSKIGNKLFVKFDTKLQFNPGNTIDVIIDNDRRTFSIASAPWENFTMIATNIRNSPFKQKLLMLKEGDEVKIEGPYQDEFVIKDSPKHVFVTKDIGITPVRSMALYLLKNSQNVTIYYQPDEDGVILFKEDLGNIMKIEQISNKIFKENQDAMFYISGEPEFTKIATKIAVDNGIKAGKIVVEPFSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W4</accession>
    <name>F4B8W4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Primase X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775750.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W4"/>
    <dbReference type="STRING" id="933801.Ahos_0948"/>
    <dbReference type="GeneID" id="10600420"/>
    <dbReference type="KEGG" id="aho:Ahos_0948"/>
    <dbReference type="eggNOG" id="arCOG05924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118423_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040865">
        <property type="entry name" value="PriX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033412">
        <property type="entry name" value="primase_PriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18689">
        <property type="entry name" value="PriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Primase X" evidence="1">
        <location>
            <begin position="45"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="16208" checksum="FB6F8D5CB54A0A29" modified="2011-06-28" version="1">MHYPDDTPAGYVIYQDGTSRVYDEDGNLLFEVEGVFPPIPSKVNYSWIDTILEKGLPDGRKRFILYVASRYLVNVKNLNEDEALEKLKEFYYKTGSGKIYDAWIRSVIKGVKTKGLRPPSLKKLQEKDPELYEEIRRVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B916</accession>
    <name>F4B916_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine gamma-synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776954.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B916"/>
    <dbReference type="STRING" id="933801.Ahos_2168"/>
    <dbReference type="GeneID" id="10601671"/>
    <dbReference type="KEGG" id="aho:Ahos_2168"/>
    <dbReference type="eggNOG" id="arCOG00060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_2"/>
    <dbReference type="OrthoDB" id="43458at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000033">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005047">
        <property type="entry name" value="PRK06460.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="375" mass="41363" checksum="7C3066C8FCAD199D" modified="2011-06-28" version="1">MKDSTKAVKEDIEDSTKAITTPIYQTVAFMFPEGEKFRYSREANPTTLELGKKIAELEGAEMGIAFSSGMGAITTTLFTLLTPGKSLLVTMDMFGRSLRFCKDFLANWGVKVTISKPGNENLLEAAKRSHFDVIFVESITNPLLRVINLPELSKIAKENNSILVVDSTFATPINQKPIEFGADIVIHSASKFIAGHNDVIAGLAAGKEVLMKNIDLMRRTLGTSLEPFPAYLVIRGMKTLKVRMDVINRNAEQIAEFLEDHPKVTKVYYPGLKSHPDYEIARKVLKGFGGVVSFEVRGGRDEALKVMRSTKVIIPAQSLGGVNSLISHPPTMSHRTLSLEERKIIGINDSLLRLSVGIEDVEDLIEDLDQALSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B951</accession>
    <name>F4B951_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776989.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B951"/>
    <dbReference type="STRING" id="933801.Ahos_2203"/>
    <dbReference type="GeneID" id="10601708"/>
    <dbReference type="KEGG" id="aho:Ahos_2203"/>
    <dbReference type="eggNOG" id="arCOG07195">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2712923_0_0_2"/>
    <dbReference type="OrthoDB" id="39466at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="65" mass="7796" checksum="64556DD3EC203490" modified="2011-06-28" version="1">MNSIVDLDSDQCSFDPLEAIEYLKDKKEYVIFKISMNNPFLQDIKRKYFLQIIKVDGEIIYFKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B969</accession>
    <name>F4B969_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="9">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trpS</name>
        <name evidence="12" type="ordered locus">Ahos_0976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775778.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B969"/>
    <dbReference type="STRING" id="933801.Ahos_0976"/>
    <dbReference type="GeneID" id="10600448"/>
    <dbReference type="KEGG" id="aho:Ahos_0976"/>
    <dbReference type="eggNOG" id="arCOG01887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032621_0_1_2"/>
    <dbReference type="OrthoDB" id="371821at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000007">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000138">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008924">
        <property type="entry name" value="PRK12285.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008927">
        <property type="entry name" value="PRK12285.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="81"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="380" mass="44485" checksum="DB8CA7C2E8F6AA9E" modified="2011-06-28" version="1">MSSNFSVTPWEVKGKVDYDKLIVQFGTQKITPELKERIKKITGDLHVMLRRDVFFSHRDLDLVLKDYEEGKGFFLYTGRAPSLGMHIGHLVPFIFTRWLQEKFKVNLYIEITDDEKFMRNPELTLEQTRQFAYDNILDIIAVGFDPDRTFIFQDTEYIRNMYPLATKIAKKLTFSEVRATFGLENSSNIGIIFYPALQIAPTMFEKKRCLIPAGIDQDPYWRLQRDIAESLGYYKAAQIHSKFLPPLTGPEGKMSSSIPESAIYLTDDPKTVERKIMKYAFSGGQPTIELHRKYGGNPEIDVSFQWLYMFFEEDDNKIKKIEEDYRSGKMLTGELKQILIDKLNKFLEEHRAKREEAKELVNTFKYEGKLAKEMWNKIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B975</accession>
    <name>F4B975_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bacterioopsin activator HTH domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775784.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B975"/>
    <dbReference type="STRING" id="933801.Ahos_0982"/>
    <dbReference type="GeneID" id="10600454"/>
    <dbReference type="KEGG" id="aho:Ahos_0982"/>
    <dbReference type="eggNOG" id="arCOG02271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101251_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007050">
        <property type="entry name" value="HTH_bacterioopsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34236:SF1">
        <property type="entry name" value="DIMETHYL SULFOXIDE REDUCTASE TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04967">
        <property type="entry name" value="HTH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH bat-type" evidence="1">
        <location>
            <begin position="133"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="192" mass="22464" checksum="68744241185E853D" modified="2011-06-28" version="1">MLYVSFKVNHEDWSKGINYSRNSLSVLDIKPSTNGARLLIEFKGKEEDAKKLKWNLSRVSKFKYLGTIIVNEPIERILSNYIIMNGIATNDGIYWTVILSDYTELKKMLKDFVEHHIEVKVVKVIKVKSEDVLTARQEQILKIAFEAGYFDYPKKIRIKELAEKLNMSVSNLSEILRRAERNVIETFFRERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B2</accession>
    <name>F4B9B2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-2 type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1019</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775821.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9B2"/>
    <dbReference type="STRING" id="933801.Ahos_1019"/>
    <dbReference type="GeneID" id="10600494"/>
    <dbReference type="KEGG" id="aho:Ahos_1019"/>
    <dbReference type="eggNOG" id="arCOG01467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_2_3_2"/>
    <dbReference type="OrthoDB" id="147058at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051328">
        <property type="entry name" value="T7SS_ABC-Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077:SF10">
        <property type="entry name" value="TRANSPORT PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43077">
        <property type="entry name" value="TRANSPORT PERMEASE YVFS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="6">
        <location>
            <begin position="21"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="28683" checksum="A27D7D7A6424DD63" modified="2011-06-28" version="1">MIEGLEALYMREVKRIYRSIYMWIMIISQPVMWLVFFGSSLSGLPKEVLISFFHTTNYIAFILPGELSVSMLFVGMFSSMSLIQDKRFGYMKRIFITPVPKYYVFLAKVFGGITRGLLQVPILIGTAVVMGVSLNINLISILTIVLSLTFLGMGFSSLYAIFTIRTSDWQAPGVIANLLNLPLMFSSTALFPRAFFPSWLSDISDVNPITYSAELGREVLLSGDPNWSYLGFLAIFGLIMLILGSYLTNKYMQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C1</accession>
    <name>F4B9C1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycogen debranching enzyme GlgX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1028</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775830.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C1"/>
    <dbReference type="STRING" id="933801.Ahos_1028"/>
    <dbReference type="GeneID" id="10600503"/>
    <dbReference type="KEGG" id="aho:Ahos_1028"/>
    <dbReference type="eggNOG" id="arCOG02949">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011725_1_1_2"/>
    <dbReference type="OrthoDB" id="18347at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11326">
        <property type="entry name" value="AmyAc_Glg_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02856">
        <property type="entry name" value="E_set_GDE_Isoamylase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000054">
        <property type="entry name" value="Glycogen debranching enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044505">
        <property type="entry name" value="GlgX_Isoamylase_N_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011837">
        <property type="entry name" value="Glycogen_debranch_GlgX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02100">
        <property type="entry name" value="glgX_debranch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43002">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="171"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="710" mass="81440" checksum="E1991AC5B76D4436" modified="2011-06-28" version="1">MAFVQIPTRPGYPYPLGVSLTKDGANFVLFSENATAVQLLLFSHPDDKEPKEVIEVKEKTGDLWHVCVPGIMPGQLYAYKVDGPYKPEQGLRFNKNKTLIDPYAKAVAGFIKWDDSLFGYKIGDPNQDLSFDERDSTPFIPKSVVVSDEFDWEDDKPLSLRIPWSQTVIYETHVKGFSIKRDDIDEKIRGKFLGLASKSAISYFKDLGITAVELMPVQQFVRDRFLVEKGLTNYWGYNPIAYFAPDCGYATDCLGKQVVEFKKMVKELHAAGIEVIIDVVYNHTGEGNQLGPTLSFRGIDNSSYYMLNPQNPRYYMDFTGAGNTLNLRHPRVLQMVLDSLRYWVLEMHVDGFRFDLASALARELYSVNMLSTFFVAIQQDPILSRVKLIAEPWDVGEGGYQVGNFPYQWAEWNGKYRDTIRRFWRGEPMLYSELANRLMGSPDLYQGSGRTPFASINYVTSHDGFTLQDLVSYNQKHNEANGFDNKDGMDENYSWNCGFEGETQDPNIIACREKQKRNFMITLFISQGVPMLLGGDELSRTQKGNNNAFCQDNEISWYDWNLDERKKAFRDFVKSMIYFRKAHPIFRRRKFFQGRKLFGSPYKDLTWISPAGTEIDDKTWNSPTQTIAFVLSGDAMDEVNERGERVADDTFLVILNGSPSSIKFKIPKLGEKWELVAWSCLREPKENEKIVKAEQEIQVEGRTAIIYKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I2</accession>
    <name>F4B9I2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF790 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777043.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I2"/>
    <dbReference type="STRING" id="933801.Ahos_2257"/>
    <dbReference type="GeneID" id="10601763"/>
    <dbReference type="KEGG" id="aho:Ahos_2257"/>
    <dbReference type="eggNOG" id="arCOG04356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038336_0_0_2"/>
    <dbReference type="OrthoDB" id="57367at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008508">
        <property type="entry name" value="Bax1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640:SF1">
        <property type="entry name" value="DUF790 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39640">
        <property type="entry name" value="VNG6129C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05626">
        <property type="entry name" value="DUF790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019435">
        <property type="entry name" value="UCP019435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="57214" checksum="A0EC798C3D4A3307" modified="2011-06-28" version="1">MLPSELARYKIQGNKVIPLFATEEDEALARNIIEMFKEGRKLGDILDEIKILEKAYDTPCKVKLIRGFYKEMLRLCTFSEDSPIDPRIIRREVFSYGPITSKEKREKILREVEEKLKVDVEKYMFSDMEEEKTVSSVNKISPISLIKMYNLSLLQTLLFKSYKMTVQIEGNWKEIIKRIKWLGLMYYAYPNPIRIDIIGPATLVKMTEKYGRNMAVILPYIVSSKHWKINAELVLGKKIKRTFLLTVEKFDLIDSKVREEDEKRFDSSVEERFFNDFQKVIKDWKIYREPEALVINGRLFIPDFLVEKNPFKIYVEIVGFWTKEYIREKLEKLRNFKGGEILVLLNQELSKEDFSDFNVIKYKNRVDIGLVYRWLKDYERKNAESVNISYSLEGDVISLKDLSRKLNVSEEIIKKNIHNDKYILTGNYFVRKDFLEKLKEENFENKKLSELIKIYGDYITEVLEYLGYKFKWLGITDAVVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J9</accession>
    <name>F4B9J9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycogen debranching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777060.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J9"/>
    <dbReference type="STRING" id="933801.Ahos_2274"/>
    <dbReference type="GeneID" id="10601780"/>
    <dbReference type="KEGG" id="aho:Ahos_2274"/>
    <dbReference type="eggNOG" id="arCOG03287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026835_0_0_2"/>
    <dbReference type="OrthoDB" id="8543at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004135">
        <property type="term" value="F:amylo-alpha-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010401">
        <property type="entry name" value="AGL/Gdb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032790">
        <property type="entry name" value="GDE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006451">
        <property type="entry name" value="Glycogen_debranch_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024742">
        <property type="entry name" value="Glycogen_debranch_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01561">
        <property type="entry name" value="gde_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10569:SF2">
        <property type="entry name" value="GLYCOGEN DEBRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06202">
        <property type="entry name" value="GDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12439">
        <property type="entry name" value="GDE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycogen debranching enzyme bacterial and archaeal type N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Glycogen debranching enzyme C-terminal" evidence="1">
        <location>
            <begin position="250"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="611" mass="71092" checksum="B8EA2A2933CEF58A" modified="2011-06-28" version="1">MINPEECEDREWIIPTGTGGYSSSTICGINSRTYHGYLIVPKNPPHFRHLVLSKFEDFFIQNGIEYPISTNRYNFQVYYPEGYKFLNRFILGSNFVVWEYNFENSLVRKTLVVNKGTNSVTITYESDKGLFKICPLITYRSHHIALKERPGFFDFQTKGDTIVVTLNDNKILNFKIEGEFNIENTGYWYYNFFYKLDYERGSNYLEDLYNPFCIITKNNEITITAYVDSFVKSTKIHLRKDVLRMLSSAGRDFVVKGKDGWAIIAGYHWFDEWGRDSFISMEGLVLLNGEYSIAKDIISRYLEYEERGFLPNGFLHNGEPIYKGVDVSLWAINAIYKYYVYSRDLDFIKKIFPKLIEIIDWYWKGNGIVETINGLLFHTGSPRTWMDAEFDTTIVTPREGAAVEINALWYNALKIMDFLSKELKINSDEFEIKAEKVKAQFIEKFVSPWGLYDYLNPDLTPDSSIRPNQLFAFSLPFNVVDENIGKRIVNTVEKELLRPYGLSTLSKSDKKYIPFYRGDRRSRDLAYHNGPIWPWLIGSYIDAKIKLERGNIIGIKKLINYLQPLINVAMENNGYIPELFEDIPPYKWGGCIAQAWSVAEVFRSLNNIISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V1</accession>
    <name>F4B9V1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777082.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V1"/>
    <dbReference type="STRING" id="933801.Ahos_2296"/>
    <dbReference type="GeneID" id="10601804"/>
    <dbReference type="KEGG" id="aho:Ahos_2296"/>
    <dbReference type="eggNOG" id="arCOG05887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709216_0_0_2"/>
    <dbReference type="OrthoDB" id="35349at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="18021" checksum="881EF12DCC4BFCD7" modified="2011-06-28" version="1">MENFRELIIDAVLSKRIKNFKDIESEGTRKRNICSYFNGKICRIKFEESVVSSWLSQEGITPHPVLCYICPYFSLKEEEESIKSSLFDIYAYYAKLKESVEKELQFIESRVNNFVYSSVSLKRRREELIYLLDDINEKIKITTELIKLSEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W8</accession>
    <name>F4B9W8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation diffusion facilitator family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2313</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777099.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W8"/>
    <dbReference type="STRING" id="933801.Ahos_2313"/>
    <dbReference type="GeneID" id="10601821"/>
    <dbReference type="KEGG" id="aho:Ahos_2313"/>
    <dbReference type="eggNOG" id="arCOG01478">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076277_0_0_2"/>
    <dbReference type="OrthoDB" id="8907at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF30">
        <property type="entry name" value="TRANSPORT PROTEIN, HYPOTHETICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="7">
        <location>
            <begin position="20"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="326" mass="37498" checksum="8135B66D2ABC4751" modified="2011-06-28" version="1">MIPLEKLREVSRIFLFTGALLLPISIIEIYFGEIYHSAILIADSFHGFIDATSAILFSLLLNIIYRRSNKFPWGLYNLESIAILFVTIFIVILSINYINLIIKDVNYEVPAWLSLIIYSSSVLTLVVFILEKRYSWISLVKTDLTHSKLDLFMEIVSGFAIIINEYYLTLAVVFTIIGFILADAIRQFKEAIYSLIGINCDAPFKDRIKIILESLGINLKNIYVRKLGSFYMVYVVISLPPDCKLSNVYKIRKTVKRIVNSFENVAMVEVRVVPEKIKNHKLLITNDATKVSNSNKVNEANWAKYSSNFKDIENRRELIKRTVTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X5</accession>
    <name>F4B9X5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X5"/>
    <dbReference type="STRING" id="933801.Ahos_2320"/>
    <dbReference type="KEGG" id="aho:Ahos_2320"/>
    <dbReference type="eggNOG" id="arCOG05885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168020_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="108" mass="12239" checksum="3140C05C76D8E44B" modified="2011-06-28" version="1">MLLVIPYFILWGLWGKYFRKIVTEGLKYVGDEYVDENYKIPPLSSVKIAINGKCAIVINGGTGWATIRINGGPREKVFKIRLLNAVGILEIINESRFFPIIVSIRRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA06</accession>
    <name>F4BA06_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase S53 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2351</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA06"/>
    <dbReference type="STRING" id="933801.Ahos_2351"/>
    <dbReference type="KEGG" id="aho:Ahos_2351"/>
    <dbReference type="eggNOG" id="arCOG03665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006754_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S53" evidence="8">
        <location>
            <begin position="162"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE95222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1052" mass="115560" checksum="A22610CD0B451379" modified="2011-06-28" version="1">MAILITLLIPFYSPSNTSNYHNLQELNPNCIVTVAIVEQPKNLAKLQLDIENHIILNKTCVYKLFIPNNEIIKAIAYLTQFGIQTSVYMNVILATGKVCSFEKALNGKFYVINFHDIKFYEFFGDPPLSIGNALVFSTNITSQILQKPNTLYNYSQAIAYNVIYPCILREAYNVTLLYSRGIMGNGTNIGIIDFCGDPYIYQQLICFDKKFNIQNPPRFIVCPIGPYNPNQGIESGWALEISLDVEYSHSIAPAAGMILYVANPNVPLPAIIAFVDQQDKVSVVSQSFGIPEIYFDLGLIPLSYLQSLTYEYWLGEVEGITFVAASGDYGGNGYNFFLSPQGGLLIPASDPYVLAVGGSTLYYSCNKTIQEAWSGESIYGASTGGFSSVFPSPWYQGVKGFREVPDVIADANPYTGVPVIYYYGEEYLIGGTSVASPIVAGIIDLATQVHGKFGFINPLIYSLKGTKALEQVTFGYNTPYYIKNNSYNPVSGLGYINAGCFVSLIRNESTISVAVSNITYNDGQLVKVIVKASPLVPVTGYVYNGSNILEKFPLVYNGTYWIGYFTAKGSGIQEVVVTQGNDKAGTYILVGLQAMFLCPQVALYPSPIGIKVIAELLYANGSAAKANGVFTAFLYQYNITTCTFEIINKTNLSAENLIQGYLYIIVNNGSYIYGCFAPTTPFGGIYIIRISCVFGFSEFVEGIYLIPYIIPAAFTEPVSIADGCNFTIGIALLSLVAPNVTVKLYNADNQLVYSFSINAISYANSIFYVGEISLPHNISRGYYKIVSVANYNSTDHMVYGEGFTQVYISSCTLRVIVTTIPSGNIYENETLSIIASITYENGTNVNFGTFNAVIIPSYMKDNFDSLDLEFSVPLYYAKGEWFGKVTTPCEKSNPLGLSAEGLSGEWCIYVYGTSYNGIPISFPTTLSYSTLNIIPSIPNDQFILLPYTFIKDFNGTLMKDAYICNAVIINHNATLVNSIIKNLTIINGSVYLINSRICSYKTINGKVLNENATTKICNNEVNNSLSNLSESLALLAGLLAIDVVIIKKFRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA33</accession>
    <name>F4BA33_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Histidinol dehydrogenase</fullName>
            <shortName evidence="10">HDH</shortName>
            <ecNumber evidence="4 10">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_1146</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 15">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="4 10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775946.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA33"/>
    <dbReference type="STRING" id="933801.Ahos_1146"/>
    <dbReference type="GeneID" id="10600620"/>
    <dbReference type="KEGG" id="aho:Ahos_1146"/>
    <dbReference type="eggNOG" id="arCOG04352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_3_2"/>
    <dbReference type="OrthoDB" id="36308at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000026">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE94030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="396" mass="42861" checksum="D3F5C89ACA5D4223" modified="2011-06-28" version="1">MIIKELPKSRPISFDQVLDKVSEIVGKVKREGDKALIEFTEKFDGIKLDNIKVTKEEIHAAASLLSEDIKKAIDVVSEQIKEFHTSIKPPQIGGGKSGVDFGIIWTPLEKVGIYVPGGKKLYPSTLLMAGIPAIVAGVEEIYVATPTKGKIDPAIAYIAEKLNVKEIYKIGGAQAIAALAYGTESVKKVDKIVGPGNVYVQAAKYIVSKDVGIDGIEGPTELVIVADDSANSEEIYLDLLAQGEHGNSTLLVLISTSDRILREVEEKVSGSDLTFYLVKARDLEEAITYANSIAPEHLSLQVSSPREALKMVRNAGAVTLGRTPPAIIDYSAGPDHILPTNSWAKFKGGLTVYDFLKPISFAQATNPDKELINAAITLARYEGFEIHSKSIGERYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA36</accession>
    <name>F4BA36_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775949.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA36"/>
    <dbReference type="STRING" id="933801.Ahos_1149"/>
    <dbReference type="GeneID" id="10600623"/>
    <dbReference type="KEGG" id="aho:Ahos_1149"/>
    <dbReference type="eggNOG" id="arCOG00618">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010113">
        <property type="entry name" value="PRK13586.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="230" mass="25341" checksum="BE1DB4766DEFEA02" modified="2011-06-28" version="1">MMYVVPSIDISQGKAVKRVRGVKGTGIIIGDPIKVAENIYSLGYDTVHIVDLDAAENLGNNEEIIAGIARIGFKRIEVGGGIRSIEKANRMLSLGITEIVVSSILFTNKEEFDKMSELLGDRLFFSIDYCEGKTLIKGWKEEAKSVDETISLLKNYDIKGVIFTYVCNEGTKKGIDENISMYSSRVNKLKGYAGGINNINDLLKLKESKIDFAIVGMSFYSGSLKGVKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA48</accession>
    <name>F4BA48_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP-binding protein HSR1-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775961.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA48"/>
    <dbReference type="STRING" id="933801.Ahos_1162"/>
    <dbReference type="GeneID" id="10600636"/>
    <dbReference type="KEGG" id="aho:Ahos_1162"/>
    <dbReference type="eggNOG" id="arCOG00350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_1_2_2"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01859">
        <property type="entry name" value="MJ1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016478">
        <property type="entry name" value="GTPase_MTG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006230">
        <property type="entry name" value="MG442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="30234" checksum="63BDF73A89889C6F" modified="2011-06-28" version="1">MLGDVLKVIKKSDAVLEIVDSREPDLTRSKKIENIVKKEGKGLLIVINKGDLVPREVLEKWKRYIEKEDKIKTVYISATSHLGTKILRNAIKEVLKGNEGTICLVGYPKTGKSSIINALKGRHSASTSAHPMAYGYTKTIQQFKVDSKIYAWDTPGIIPPDGSPLERAIRGQAVEKIEDPVKVALLLIDRIEKFDKNALKSAYKLDYSDGLDLLQKIAIKRGWFYKKDKEPLIEEAARQLIRDYHEGKIIYFTLPPSESNDENKSSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA53</accession>
    <name>F4BA53_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Crossover junction endodeoxyribonuclease Hjc</fullName>
            <shortName evidence="11">Hjc</shortName>
            <ecNumber evidence="11">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Holliday junction resolvase Hjc</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hjc</name>
        <name evidence="12" type="ordered locus">Ahos_1167</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775966.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA53"/>
    <dbReference type="STRING" id="933801.Ahos_1167"/>
    <dbReference type="GeneID" id="10600641"/>
    <dbReference type="KEGG" id="aho:Ahos_1167"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139546_1_0_2"/>
    <dbReference type="OrthoDB" id="34330at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="Holliday_junction_resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040854">
        <property type="entry name" value="Hol_resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651:SF1">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="144" mass="16186" checksum="E676175F31781EC7" modified="2011-06-28" version="1">MNERKRKGTSVEHYILSSLRDKGFAVVRAPASGSKRKDPIPDIIAMKNGVILLIEVKSRKEKNKVYVPRSQAEGIINFAKKSGGELLLAVKFPKFLKFIPFSKLRITESGNYVADEEVIEEGLSLEDLVRAVEAKFSKTLDFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA54</accession>
    <name>F4BA54_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleotide binding protein PINc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775967.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA54"/>
    <dbReference type="STRING" id="933801.Ahos_1168"/>
    <dbReference type="GeneID" id="10600642"/>
    <dbReference type="KEGG" id="aho:Ahos_1168"/>
    <dbReference type="eggNOG" id="arCOG04116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052041">
        <property type="entry name" value="Nucleic_acid_metab_PIN/TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010335">
        <property type="entry name" value="PRK13764.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603:SF147">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="3">
        <location>
            <begin position="2"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="245"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00046345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="508" mass="57292" checksum="7FC22C219E5F72B2" modified="2011-06-28" version="1">MSELLVDKSALLLGLSKYIEKGLINGNILIHRALLSELEKETRDGLISGEIALDEIEKVRKLSERYLFSVEIVGEPSSNVDNALREYCSQRDCSIVTADEIQKQICSYLGIKVVYLQPFEGSLSIEKFFDESTMSVHLKEDTIPKAKKGKPGNWQFVNLSNIPMSADEIKSLVGELINEIKYVKNSFIEIERKGSTIVQLGNYRVVITRPPLSDGWEVTITRPVTKKSLEDYNLPEKLIERLKERAEGILIAGSPGMGKTTFAQALAEYYMKLGKVVKTIESPRDMHLPPDITQYSKNYAEIGELHDILLLSRPDYTVYDEMRNDDDFRLYIDLRLAGIGMIGVVHATTPIDAIHRFLNRVDLGTIPGILDTVIFISEGTVKKVYSLDMTVKVPLGLREADLARPVVEIKDFLTDQVEYEIYVFGEQTMIVPVSKTKGGNSVENKIAKTVMDSIPGAEVKYENGEYVITIPKTEIGKFNRRLNSKLRKLEKKHGVRIRIRLGDSDEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P1</accession>
    <name>F4B3P1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylglutaconyl-CoA hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774847.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P1"/>
    <dbReference type="STRING" id="933801.Ahos_0035"/>
    <dbReference type="GeneID" id="10599475"/>
    <dbReference type="KEGG" id="aho:Ahos_0035"/>
    <dbReference type="eggNOG" id="arCOG00242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051683">
        <property type="entry name" value="Enoyl-CoA_Hydratase/Isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42964">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42964:SF1">
        <property type="entry name" value="POLYKETIDE BIOSYNTHESIS ENOYL-COA HYDRATASE PKSH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="256" mass="28834" checksum="08B50E4726D7CB55" modified="2011-06-28" version="1">MFVNKEYKDNFILLTFNTGTKYNVFNVKFMTELLDTLSEIEKEKKYRFIVFKGENGNFGSGADIRELNRASIDREFASSFFNYMKDLYLKLINIDKITIAQVEGLAYGASLELLLVMDFVIASKSAKFAAPGGKIGVFPPVLLTLGPYIIGLNNVKRLAMLGEEITAEEAEKIGLITKATDEIDKETANLIYKMSFMAPSSLMLMKRHIMKYLDRDLSEVFKSLIIQVGGDESKEGINAFLAKTKPSWLVNLSKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3S8</accession>
    <name>F4B3S8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3S8"/>
    <dbReference type="KEGG" id="aho:Ahos_1232"/>
    <dbReference type="HOGENOM" id="CLU_2839165_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="67" mass="7624" checksum="E2FCDA3155D3BD3A" modified="2011-06-28" version="1">MNSFLSISPLTKNLKIHLSQTTLKDIENVFKNISLFVYYPTKNNSCNNDNSGSSNHSWDYVLLQTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V7</accession>
    <name>F4B3V7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase/mu-crystallin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776060.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V7"/>
    <dbReference type="STRING" id="933801.Ahos_1261"/>
    <dbReference type="GeneID" id="10600742"/>
    <dbReference type="KEGG" id="aho:Ahos_1261"/>
    <dbReference type="eggNOG" id="arCOG01035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_1_2_2"/>
    <dbReference type="OrthoDB" id="21421at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="300" mass="32887" checksum="F0187147BF1AF0FD" modified="2011-06-28" version="1">MLYLDGKKLEEILSAKDAVNAVKEAFSLYYQGKVTQPQRQVLTIKGNWWGIMPSYTDFSVVVKIVNVINENKNRGLPSVQGIVILMSPDTGEPLAVIDGTILTAIRTASASVLSTELSYGNRIPTLGIIGAGLEAYYHAKIASEYLSISRILITARKSHIELAKKIGADAVDLQTLLKESDVIFSTTSSKEPVVLGKFLKEDFHVSSIGAHTPDAREIDDETIAKAKTYIVDSLQAVSSESGDYIQPQRKGLIKNVIEIGKIITENIKIERPSIFKTVGISAQDNLTAYYAYKLSQTFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X2</accession>
    <name>F4B3X2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774853.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X2"/>
    <dbReference type="GeneID" id="10599481"/>
    <dbReference type="KEGG" id="aho:Ahos_0041"/>
    <dbReference type="eggNOG" id="arCOG11952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194240_0_0_2"/>
    <dbReference type="OrthoDB" id="38293at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="86" mass="10262" checksum="99717C60836EC962" modified="2011-06-28" version="1">MKKIYVFYTPKRIVNSEDYEVEILEKVSKKFKLGRLLRYDSVSYDEGGITYLKGIFERGKAIVKFKEGEEAIALVKKYKRTFRIWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B415</accession>
    <name>F4B415_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AAA ATPase central domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0086</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774896.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B415"/>
    <dbReference type="STRING" id="933801.Ahos_0086"/>
    <dbReference type="GeneID" id="10599526"/>
    <dbReference type="KEGG" id="aho:Ahos_0086"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_1_2"/>
    <dbReference type="OrthoDB" id="36037at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF199">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="48"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="315"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE92979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="540" mass="61790" checksum="0CB941E14FD00C9F" modified="2011-06-28" version="1">MMIRANRIANKHKKVISYIPSTRLEDLYDLKDVKKRLEEIAEEAKKGKTYGVILFGPPGTGKTSLSKAIANKLGWNFFQLNASDILSKWYGESEILLTSFLDKVESNQPAVLFIDEIDSFTMSREDDVHEVTHRLINILLNRIQEFHDKGDKILIIGTTNLPQEIDEAFLRPGRFDEIIYVPLPDEEGREEIWKGYIKEPNIDYKLLAKRSERFSPADIKLIVDEVKSKIQNPTTEDYLKFIENFKPSVKISTLIKFENLAKKYSREKMIDKPFGVPDITWDDLGDLEKVKEIIRESVELPIKNREFAEKLGIKPVKGILLYGPPGTGKTSIAKAMANELKASFIILSGEEIASAQIRAPEVIAEKFNIARDNSPAVIFIDEIDMIAKNRMFNEWRNALTELLTQIDGIRETDDIILVGATNRPWDLDPAILRPGRIDKLVYVPPPDYEGRIKVLKVLTKGLEVDEKTIEEVAKITENYTPADLKLVVDEIRRNLLKEASITNVLRIKVNIDDFKKVLDKVKPSVTKEEIKMYESFKARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B478</accession>
    <name>F4B478_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA C-acetyltransferase (Acetoacetyl-CoAthiolase) (AcaB-1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776102.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B478"/>
    <dbReference type="STRING" id="933801.Ahos_1303"/>
    <dbReference type="GeneID" id="10600785"/>
    <dbReference type="KEGG" id="aho:Ahos_1303"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="244"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="355" mass="39207" checksum="9FFDF106451FCE4F" modified="2011-06-28" version="1">MTDVLVYDSSMIKIDRYYEKNLVDLVAEVMSKFNSQIKPDAIILANAYSETTVNQVLLASKVSSMLGFSGFTTRIENGDASGGAAIETAYYMIKSGLVKSVLLIGVEKMGDFPTSYLNEITAENLDEKFGYREGLTLQSFAALQMKQYMKTYGVDYDYFTQWPLAMHKYGSENPFAYLKFQVDKKTIEESQIISEPLRLFDTSPRADGAAAVLLTNEEFKPEIKVKITGVSSAVSEFTTLSMPAVRQAYSQFSEYKADFMEIHDSYSINAALILEEIGFEKGKSLNMLDSIQVNYSGGLKSRGYPGGATGIYQLAESFMQLAGSFPGKRAQGEKGVIISTDDLGTSAYVIGLKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B480</accession>
    <name>F4B480_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="5">HMG-CoA reductase</shortName>
            <ecNumber evidence="5">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776104.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B480"/>
    <dbReference type="STRING" id="933801.Ahos_1305"/>
    <dbReference type="GeneID" id="10600787"/>
    <dbReference type="KEGG" id="aho:Ahos_1305"/>
    <dbReference type="eggNOG" id="arCOG04260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="OrthoDB" id="10981at2157"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="409" mass="43760" checksum="489985D56D5BC505" modified="2011-06-28" version="1">MNIDEIVEKAVKGEIELHEIDNFLEANAAMVARRLALEKILGISLPSIGSTILDYSEIKNRNAENVIGGIQIPVGIVGPLKVNGDYAKGNFYVPLATTEGALIASVNRGCKAITKAGGAKVKIIFDGMARAPIFKLPNIDSVVNFLQWINKNLDKIKEVAESTTSHGKLKEIQPFVLGNNVWLRFVFETGDAMGMNMATIASEAICNFIESEFTEAKCIAVSGNMCSDKKQSMVNSIFGRGKTVIAETLIPSTILKEVLHTTAEAIHEVNLRKNLLGSARAGNVYQFNAHFANIIAAIFLATGQDMAQVVESSSGYTWTEVRGEDLYISVTLPSLEVGTVGGGTRLPTQREALSIMGVAGGGNPPGSNVKKFAEIIASTVLAGELNLLSALSNKELGRAHKMLGRGIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B481</accession>
    <name>F4B481_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="9">SHMT</shortName>
            <shortName evidence="9">Serine methylase</shortName>
            <ecNumber evidence="9">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glyA</name>
        <name evidence="12" type="ordered locus">Ahos_1306</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reversible interconversion of serine and glycine with a modified folate serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776105.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B481"/>
    <dbReference type="STRING" id="933801.Ahos_1306"/>
    <dbReference type="GeneID" id="10600788"/>
    <dbReference type="KEGG" id="aho:Ahos_1306"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="OrthoDB" id="5821at2157"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000101">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000136">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="10"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="9">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9 10">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="431" mass="47967" checksum="B4C04F41631C27A5" modified="2011-06-28" version="1">MELPEELTKVIQLTKEQNTWRRTETINLIASENVMSPLAEAVYMSDFMSRYAEGKPYKRFYQGTKYVDEIESLAIELMNEVTPAKNSDLRPISGTIANAAVFRILANPGDKALIAPVQAGSHVSHTKFGTLGALGIQHIEMPFDKENINVDVDKAIKMIEEVKPKFVVLGGSLYLFPHPTKELAPHVHAVGAKLVYDAAHVYGLIVGKAWSNPLDEGADVMTTSTHKTFPGPQGGSILSNDDELFKEISRTIFPWFVSNHHLHRLPATAVTLIEMKYFGKDYASQITKNAKKLAEALAERGFKVVGEHLGYTKSHQVAVNVRELGGGAKIAKLLEEANIIVNKNLLPCDTPETVSNPSGLRIGVQEMTRYGMREDEMDEIAELMKKVAIDGKDPKEVRKEVIELRKRFLEVKYTFNVDLSSYSSKSIPMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B485</accession>
    <name>F4B485_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776109.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B485"/>
    <dbReference type="STRING" id="933801.Ahos_1310"/>
    <dbReference type="GeneID" id="10600792"/>
    <dbReference type="KEGG" id="aho:Ahos_1310"/>
    <dbReference type="eggNOG" id="arCOG05981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064043_0_0_2"/>
    <dbReference type="OrthoDB" id="42110at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="28330" checksum="0C709359B7694208" modified="2011-06-28" version="1">MPFFGIFKRNKEKEHYAYDELGEWIIISGNSKLGFLYSIISKTVSKLAKYYDLYILQFLEDSEIRNFYTIKAMVSTRSPIKDSLLSSKLSQSLSKHGTLGQIDVVKLRYCGMNYLFFKFNILLKKSKNVKEDVKVLLPPLGVSASGIPYSTKDLFKSIFEYNSNAVCQSILEFKDDNTARILANCSDYVDLEGIKYSLSYFSKDFKTSVRSSIRSVEVEIEAKDFNKHALIPLLWNNFLDIYSSSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B488</accession>
    <name>F4B488_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776112.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B488"/>
    <dbReference type="GeneID" id="10600797"/>
    <dbReference type="KEGG" id="aho:Ahos_1313"/>
    <dbReference type="eggNOG" id="arCOG01935">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1922650_0_0_2"/>
    <dbReference type="OrthoDB" id="39977at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="15039" checksum="CFA3E60C64478522" modified="2011-06-28" version="1">MRILVVDDVGNILGVFSSEDEAYNFAKEKMKEGKKIHLKVQRERLLALDKKGKEIGEFKNVEEAYEFLKDKVKEGEKVRIVIGNKDFYVTDDTGNILGVFSSEDEAYNFAKEKMKEGKKIRIIAPAELYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B495</accession>
    <name>F4B495_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FeS assembly protein SufB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B495"/>
    <dbReference type="STRING" id="933801.Ahos_1320"/>
    <dbReference type="KEGG" id="aho:Ahos_1320"/>
    <dbReference type="eggNOG" id="arCOG01715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="3">
        <location>
            <begin position="165"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="2">
        <location>
            <begin position="240"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="505" mass="57407" checksum="267ECCE77699C1C3" modified="2011-06-28" version="1">MHFLKYFTLTWKGLNVFFNRVKDGRKMTEEKVSVDINEILDAAIEAKYNRVDQIEFHRRVVESGLSRSTIEAISKAKKEPEWMLRLRLKSLELFEKLPTPNWLPDVLSSLNVSNLELYIKPDVERTDNWEQIPKEIREYYEKLGIPQSEQKVLGGLVAVLESEPIYANVKQELQKKGVIMMPPEEAMVKYPDIFKQYFMKIFPASDHKFAALHGALWSGGVFVYVPKGVKITTPVEGFFVIGTEMEGQFEHTLVIADEDSYIHFIEGCSAPQFKNFSFHDGMVELFANDNAHIKFTTIQNWSKNVINFNNKRAWANKNSVIEWVEGSLGSKYSFVYPSTILRGEGASSTSLVVTLASAEGDWKDSGSKMIHAAPHTRSRVINKNIGFNGGVNVYRGLVKVNKGAVGSKAFVKCDSLMLDEKTKAYTFPHNQVFEEDADVAHEAHTFRMSEDQLFYLMSRGISEKEATSMLVLGFMDEIMRELPFEYAAMLNKVIKIELDKLGAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E4</accession>
    <name>F4B4E4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AAA+ family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774950.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4E4"/>
    <dbReference type="STRING" id="933801.Ahos_0140"/>
    <dbReference type="GeneID" id="10599580"/>
    <dbReference type="KEGG" id="aho:Ahos_0140"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_2_0_2"/>
    <dbReference type="OrthoDB" id="371918at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF18">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="45"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="242"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="434" mass="50015" checksum="201BE85AA58E8C07" modified="2011-06-28" version="1">MSLISQMNFQNPWWIDRNKIYDDEHVKRALSSNPRFIVQPLDENLLIMGPRQVGKTTYMKTTIMSLLEREEPTKILFFSCDSLKDKEDLISLMSQYRSFINPEGGFIFLDEITYVKDWNVGLLHLFNAGYFKNTRIYVSGSSSVSLLKETLPGRPLNKIIYYPLNFRVFFNTFFTKLKATTVQIYDVKSVYNYAIKLLPYISELNRALLVYVRKGGFLATNYVGDDPINSLYEVYKDAVLSDLAKLGRDERIFKEILGKIVESYGSRISENTIAKETSIGSHNTVASYLDLSEKLFILRVFRKIEGNKVNIKSLKKVYFIDPFIFRVIKRYTKGGDIQESEIPHVIEGMVGEHLAREYEGVGYTFFKNGKEVDFVVNDIGIEVKWGGGNFRDLKMQKGYILTLDDIGIEGSKAKIPVSIFLYLISSEKVFYPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M1</accession>
    <name>F4B4M1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1368</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776166.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M1"/>
    <dbReference type="STRING" id="933801.Ahos_1368"/>
    <dbReference type="GeneID" id="10600855"/>
    <dbReference type="KEGG" id="aho:Ahos_1368"/>
    <dbReference type="eggNOG" id="arCOG02103">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112304_0_0_2"/>
    <dbReference type="OrthoDB" id="35967at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048715">
        <property type="entry name" value="CggR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029349">
        <property type="entry name" value="DUF4443"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21715">
        <property type="entry name" value="CggR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14544">
        <property type="entry name" value="DUF4443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CggR N-terminal DNA binding" evidence="6">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4443" evidence="5">
        <location>
            <begin position="103"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="205" mass="23216" checksum="EC5B37F9396885F7" modified="2011-06-28" version="1">MDILTIITEATKPRQGNKPKYDESHVLHALLVIQKEQPIGRPTLEKRLQLGDATVRTLLRRLRELGVIKVDKVGGAEITEEGKKALQEWDSVIKIGEAELKSINWDCSMIIVKGGSKILEKQKVIELRDKIIKLGADSVLISVILNNKVELPPKTEEFGMKELLEDIKNSCRLCENNDLITYILPKDLHLAYKVGLLLFENWINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4P8</accession>
    <name>F4B4P8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776193.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4P8"/>
    <dbReference type="STRING" id="933801.Ahos_1395"/>
    <dbReference type="GeneID" id="10600884"/>
    <dbReference type="KEGG" id="aho:Ahos_1395"/>
    <dbReference type="eggNOG" id="arCOG01159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080079_0_0_2"/>
    <dbReference type="OrthoDB" id="147108at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055545">
        <property type="entry name" value="DUF7121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23435">
        <property type="entry name" value="DUF7121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="35428" checksum="3CA1B5FCA4E8BBDC" modified="2011-06-28" version="1">MSNSATNSAEEAIYKKIAEIKDEISKLKQEKAQALEDLNKLKQKKLEKIEKLKGIRSQLDTVRNEFSLRLEELKKLKERKQQLLQEIQGMKKQFDEIKAILQKTQGLDIDAIERRIQSLEWRLQTSSLPLDEEKRIVQKIAELEKKLGEARKMIEIKEKGTVERAEYLAKRIELSTVRQQIAGIVTELSQKRNILNSLKEDREKTKKEIDEINKQIEEIRNKIGNINNQIKEKNNEIENYIKQLKGEKVETTSRPTREEIIEKKKKIAEEKLKSGQRLTFEELLILYGENDKEDGKDSDNIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R5</accession>
    <name>F4B4R5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">albA</name>
        <name evidence="9" type="ordered locus">Ahos_1412</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds double-stranded DNA tightly but without sequence specificity. Involved in DNA compaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Acetylated. Acetylation at Lys-26 decreases DNA-binding affinity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R5"/>
    <dbReference type="STRING" id="933801.Ahos_1412"/>
    <dbReference type="KEGG" id="aho:Ahos_1412"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000008">
        <property type="entry name" value="DNA/RNA-binding protein Alba 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00285">
        <property type="entry name" value="DNA-binding protein Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003088">
        <property type="entry name" value="PRK04015.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="8">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="7">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="108" mass="11779" checksum="0B48CAF34FB87024" modified="2011-06-28" version="1">MFNDRKSGDKMSTTTPTPSNVVLVGKKPVMNYVLAALTLLNQGVPEIVIKARGRAISKAVDTVEIVRNRFLPDKIEIKSIGIGSQVVTSQDGRQSRVSTIEVTIRKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T3</accession>
    <name>F4B4T3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775015.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T3"/>
    <dbReference type="STRING" id="933801.Ahos_0206"/>
    <dbReference type="GeneID" id="10599646"/>
    <dbReference type="KEGG" id="aho:Ahos_0206"/>
    <dbReference type="eggNOG" id="arCOG08401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199452_0_0_2"/>
    <dbReference type="OrthoDB" id="43094at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="75" mass="9243" checksum="9935F681EE68381F" modified="2011-06-28" version="1">MKSTITVNKEVKELLERKKKELEIKLNKPLSWDDFFKYIFTEREEKVPKLSNEEAEILKRIVEEDRKNWRTREFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T8</accession>
    <name>F4B4T8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T8"/>
    <dbReference type="STRING" id="933801.Ahos_0211"/>
    <dbReference type="KEGG" id="aho:Ahos_0211"/>
    <dbReference type="eggNOG" id="arCOG03671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_332793_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="836"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="793"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="859" mass="96540" checksum="18CB4852EDC2E35D" modified="2011-06-28" version="1">MVKKLIIPIILILISIILVELIQSFSFTTVENLNVNMTHRTHRLSGCNFIQLVPTTQYYNYVGYNKTRLIYCNLWYFNIRGLEVNVTTTPKCMIIISNQSILAIGYIVPGYYKEISVDYSYNLQGAFGVFLKIFCYTGGCLQENMLNSIVIAFYKPPTAHPPDAVFCYNVTFPVIINGTETSVEFGVYKSPVHLFYIPYTSLFGNITLYVNYFTENAGYDKPYFIGIFNHAAISTEKGAVAKAIVYNWFIIQCRKVPTPPMPIILKNGILHVKNETYVAQNSRIMKPQSINCIKAVSNCSNSLAYVESNFSIVRMEFDIINGENFFGAKISSSPTLFMFLYSSVEKNSSCVVYIPLIVNGKTEIVKFYEIKCGDIIYLYPQNNIEGCVTINFKVLETYFHLTEVKEISIGDVYPGFSINFVMSYQEVSPIKAKIRLNSEVPFYYINGTKYYTNETYQVCVPAIICIPNNLTVDLTRYLLKCVKICNLTLPDKLILGCGCYNITANYNIQYYLSVPFNLTGSVNGEKTIISSGWYNAGEVIQIENTTYYPCEYERYIITSIKPSSTFVINSPINVSVSFIVQFYICVKSNIILHALINGTNNTLTSGWYNAGDVIHVENITYYSCPFTRFILISVNPATSFTLDSHMVITANWLEQFYVSVNCETIAIINCRNSTFKSGYYNDGTTIKINQYQYINSTERLLILMVSPKNYCIVVNSPINVTLSKILQYYVCIVLPNGTIKGWFNVSSTITLPQKIEVNGVTYVLKGSNQIIISHSGVIKPCYLVQQCTSTTTRTSTTTSTTSTESTTSTSTTSSSTIPSTTTQSTPSQITHVSTSVYLPIVISVMIAIVIGAAILIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W9</accession>
    <name>F4B4W9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sec-independent translocation protein mttA/Hcf106</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0242</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775051.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W9"/>
    <dbReference type="STRING" id="933801.Ahos_0242"/>
    <dbReference type="GeneID" id="10599682"/>
    <dbReference type="KEGG" id="aho:Ahos_0242"/>
    <dbReference type="eggNOG" id="arCOG05911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169419_0_0_2"/>
    <dbReference type="OrthoDB" id="44155at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.3310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="112" mass="12983" checksum="60AA178C2A95CC06" modified="2011-06-28" version="1">MMGNISDTLILVIIAILLLAGEKDVVSTARNIGKTIEEFKRRQNEFKNELLRELDETTDVHRSIVKDMTYDDNYEYVRSPPQTSNEKIKELEEQINKLKSEVERLKKGDGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B509</accession>
    <name>F4B509_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromatin protein Cren7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">creN7</name>
        <name evidence="6" type="ordered locus">Ahos_1428</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">A chromatin protein, binds double-stranded DNA without sequence specificity. Constrains negative DNA supercoils.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Methylated at multiple sites, to varying extents.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Cren7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776226.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B509"/>
    <dbReference type="STRING" id="933801.Ahos_1428"/>
    <dbReference type="GeneID" id="10600920"/>
    <dbReference type="KEGG" id="aho:Ahos_1428"/>
    <dbReference type="eggNOG" id="arCOG04114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911298_0_0_2"/>
    <dbReference type="OrthoDB" id="38142at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.610">
        <property type="entry name" value="Chromatin protein Cren7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01387">
        <property type="entry name" value="Chromatin_Cren7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038647">
        <property type="entry name" value="Cren7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020906">
        <property type="entry name" value="dsDNA-bd_Cren7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11520">
        <property type="entry name" value="Cren7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="59" mass="6648" checksum="022144DB7B78AEA4" modified="2011-06-28" version="1">MSSKKAIKVKTPSGKEVELAPEKAWSLAPKGRKGVKIGLFKDPETGKYFRRKLPDDYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B520</accession>
    <name>F4B520_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plasmid stabilization system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B520"/>
    <dbReference type="KEGG" id="aho:Ahos_1439"/>
    <dbReference type="eggNOG" id="arCOG01663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157019_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="RelE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="16233" checksum="CC980B0B3403D5C0" modified="2011-06-28" version="1">MITTIIIITTIIPNEIIYEKERFLNMFSISFVVSCEKWELRFSMRKARDYREFMQYTADNFSDKLVMLILEKLELLRKDPLKYAREKLGEDKYNNTMFSIEVTRDIRILYSVNSKNCIVFIWEIGSHKKVYGHDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B575</accession>
    <name>F4B575_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776292.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B575"/>
    <dbReference type="STRING" id="933801.Ahos_1494"/>
    <dbReference type="GeneID" id="10600994"/>
    <dbReference type="KEGG" id="aho:Ahos_1494"/>
    <dbReference type="eggNOG" id="arCOG11007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1471996_0_0_2"/>
    <dbReference type="OrthoDB" id="377097at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="183" mass="20993" checksum="1F12C76DB775A2EB" modified="2011-06-28" version="1">MLESYTLIVNLLYVSLLLETSLLFYFVSRKLKNLPYLWKDARSLYLLRIFSEVLDLLSSTDLLDDGMIGANFNIKSEALQKFLEKEVKGVGSKIKLINTYISSMEKIDAYISGISSNIKEIFYLILASIISFALYLIPGFSLDGLFLGFSLGLNIISMYYTIYSYLVYRDVMKKIMEIRNSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B584</accession>
    <name>F4B584_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Maltose ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0287</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775094.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B584"/>
    <dbReference type="STRING" id="933801.Ahos_0287"/>
    <dbReference type="GeneID" id="10599727"/>
    <dbReference type="KEGG" id="aho:Ahos_0287"/>
    <dbReference type="eggNOG" id="arCOG00154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568182_0_0_2"/>
    <dbReference type="OrthoDB" id="42146at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055052">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901982">
        <property type="term" value="F:maltose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042956">
        <property type="term" value="P:maltodextrin transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015768">
        <property type="term" value="P:maltose transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061">
        <property type="entry name" value="MALTOSE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30061:SF50">
        <property type="entry name" value="MALTOSE_MALTODEXTRIN-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="515" mass="56046" checksum="F2F9BC18E591F59F" modified="2011-06-28" version="1">MKDGKNSKLALSKITAAVIVVIIIIAAVAVFYLLIKPPSSVTPTTTPTKPSVSGPLNITNATDLMKLVGLNSTPSSPVTITVWDSYSTSEDQAFNQTLAQFEKEFPWIHVDVTYGVGVGCSQFEEAAKGHDAPIVYRDTSNSGGALFCAGLLLNLSEYLPPQVFSLYLNCSISDWSIGKAVYGLPDDINYIVMFYNKQYVPYAPNTTCEMVKIAEEVNTTDHVWGIAYGASDEYGYRFAAWFAGFGGQIFTTQNGQVVPALNSTAMVDALNFWYNLTYDLHINYLAPSTGAGGAEGELFEAGKAAIIFDGPWDLNAYLQALGSNLGAAPLPVVSQTGIRAEPFLGATGWMISNPSVSGATPLQIKAALIFILFVTNCQSDYRLWCVAHDIPANKIAYNEAMAELKAGKLTPSYLNQIMEGILEQAQYTQKFPNIPQMAYYWNSFHTAASEFFAQQINAQTAAQTMESNFINCLKKNGLLSSSFEILPLPPALIDAILLLLSVFMIPEIKYKVKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5F0</accession>
    <name>F4B5F0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AEE93244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="STRING" id="933801.Ahos_0353"/>
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    <dbReference type="KEGG" id="aho:Ahos_0353"/>
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    </dbReference>
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            <begin position="3"/>
            <end position="122"/>
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    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="16184" checksum="587415DCB2770A44" modified="2011-06-28" version="1">MTIIDTSALFALYVPEKLSNFVREELEKTDECYFLDLIFYEFPNVIRKRIVRNELSEDKGKEILERGLSYINLCKLVEGRELTRKTYELSLKYNLTTYDAALIALAEKVNDVILTTDEKLLRGVRNYPEISKYFLLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G3</accession>
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    <organism evidence="3 4">
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        <lineage>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G3"/>
    <dbReference type="STRING" id="933801.Ahos_1504"/>
    <dbReference type="GeneID" id="10601004"/>
    <dbReference type="KEGG" id="aho:Ahos_1504"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107894_2_0_2"/>
    <dbReference type="OrthoDB" id="97174at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR010163">
        <property type="entry name" value="Csa3"/>
    </dbReference>
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        <property type="entry name" value="Csa3_N"/>
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    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="NCBIfam" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="208" mass="23300" checksum="EAC7185C7B7D92B1" modified="2011-06-28" version="1">MILVTTFGFDEKFQIRAVLNYGKNIEKVIIVTSITDSDKVKKALSSFTTFLDNSSISYEIVKVDPINFINSVAVIAYYLPTDKKIIANLSGGMRAIIMETLTALALKGVDADVEIETEDFQGKISFKVSDLTRGDINTDHLLILREIWRGNRSIYKLAKATGMSTSAVSRKLSKLLKYGYIRREDTSYILNNKGMIMALMMEMADENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H2</accession>
    <name>F4B5H2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776311.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H2"/>
    <dbReference type="GeneID" id="10601013"/>
    <dbReference type="KEGG" id="aho:Ahos_1513"/>
    <dbReference type="eggNOG" id="arCOG11043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1965424_0_0_2"/>
    <dbReference type="OrthoDB" id="43903at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14172" checksum="AD61F4A9F1BFED74" modified="2011-06-28" version="1">MIKKILSGFIGGLLEIIFFINNLSVFSTISYHILRIDSVILGIILHLIAAIIIALVGISIIEVTKVRYENRNFLSALIMGILFGSAVLGLFSLPVHLLFFPIKITLAYVLAHIFYGIITYLVYSFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I0</accession>
    <name>F4B5I0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1521</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I0"/>
    <dbReference type="STRING" id="933801.Ahos_1521"/>
    <dbReference type="KEGG" id="aho:Ahos_1521"/>
    <dbReference type="eggNOG" id="arCOG02730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153004_0_0_2"/>
    <dbReference type="OrthoDB" id="90145at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39664:SF2">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN, CONTAINING PIN DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="14267" checksum="7408C1B4C09C1C15" modified="2011-06-28" version="1">MEAVVDTNFIIAVMFKDHVNHDEAIKEWEKLDKVYLPLISITEIAYFLIKHNIDLNVLNEILSDPKIEIIENSVEDISYALGKKDEIKSYDDFNDFLILSVAIRQNLPLLTFDKKLKIKMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I6</accession>
    <name>F4B5I6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </evidence>
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        </source>
    </evidence>
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</entry>
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    <accession>F4B5L6</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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        <property type="term" value="C:cytosol"/>
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    </dbReference>
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        <property type="entry name" value="Nucleoside phosphorylase domain"/>
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        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
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        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="15"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="230" mass="25193" checksum="A71653871DDD492C" modified="2011-06-28" version="1">MALITAKPGDLGEKAIIVGNLQRMKTVSSLLEDSKLVSEFAGYYTYVGKYKGEKVSVVFHGIGIPSLTLVTHDLYNLGVKEIIRFGSATGLKDVKPGEVVVPIGYSYNLGGTFYQYLNGEFTAYALTPDYELLNKVVNNLKAKQLKVRVGNVFTSDALFTHSKEFLDKLSSRNHIAVELEGAGLYFLANLLGFKAVSVHLIYRNANGESMSAEDINKTEKVIAEALLESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5T4</accession>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5T4"/>
    <dbReference type="STRING" id="933801.Ahos_0411"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="7"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="393" mass="42701" checksum="F025B1C03B42EE60" modified="2011-06-28" version="1">MQKYVHAVTSSTLAWAGNIYDLVLITYAYTFLNKIVGISYLDLTIIFSLGLIGRVIGASIFGKIADKRGRKIVALTGTAGYSIFQTFFALTVSFPLMAFLRTIEGIFMGAQWTSGTVLAIEQAPKQKLQFVNSIVQAGYALGYALTGVAYMLMSSDLTSISGYRIFMLTGSLPLILVPYIYFKVTEEFKPSVVAQKVRVRDYFPYFIRASLAMSGMFIAYLAVFSIYPDFASFSCFPAYYVGLLMAIANAIQGSSYVIFGRLSYMFGVFKLIYVGIAGLLVASFLSMPIFTSLKALPIMSAGVYLYAFAVGFWPLISGIAASSVPPEVRAFLTGTAYNIGAVAGGIVSALIGGIIEAFGMASLPYFVDGIEFASLAVVFVSMFTWPMKIENRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5T7</accession>
    <name>F4B5T7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase class-I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775218.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5T7"/>
    <dbReference type="STRING" id="933801.Ahos_0414"/>
    <dbReference type="GeneID" id="10599854"/>
    <dbReference type="KEGG" id="aho:Ahos_0414"/>
    <dbReference type="eggNOG" id="arCOG00090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054974_3_2_2"/>
    <dbReference type="OrthoDB" id="7388at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="23541" checksum="070F46A9AEA12B51" modified="2011-06-28" version="1">MKALAIYHHPVERLGNLRIKADEKFASEIEGNEDFDLLILMGGPMGVYEADKYPFIRKEIELVRRAYSESKKILGVCLGSQIIAEALGGKVIKGPYGQEVGVQEVSLIDEFKELFGTEKIKVFQLHGDTFSLPRGSRLLAYSEKYFQAFRLGKAVGVQFHVEVDSSIVKEWVETYNLDSSLIEEVKKIEQELKLYSSKLLEFLLEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A7</accession>
    <name>F4B6A7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein, ArsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775311.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6A7"/>
    <dbReference type="GeneID" id="10599948"/>
    <dbReference type="KEGG" id="aho:Ahos_0507"/>
    <dbReference type="eggNOG" id="arCOG05900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155018_0_0_2"/>
    <dbReference type="OrthoDB" id="9395at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12840">
        <property type="entry name" value="HTH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="136" mass="15325" checksum="AB14D3C71B15EDE1" modified="2011-06-28" version="1">MESINSNETRKKIYYYLLKQGKPTGLKKIQRDLGISSPSLVHYHLKRLEEQGLVKETPEGYVINKVILSEFVKVANHLIPISAFWSSFFLTSLVLEIALLLTGKIIDSAVFGSIIVGISSTLSVKELIKKYKETKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C2</accession>
    <name>F4B6C2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VapB-like antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776457.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C2"/>
    <dbReference type="GeneID" id="10601164"/>
    <dbReference type="KEGG" id="aho:Ahos_1664"/>
    <dbReference type="eggNOG" id="arCOG07289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850725_0_0_2"/>
    <dbReference type="OrthoDB" id="37380at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="15716" checksum="CF13C3424055328E" modified="2011-06-28" version="1">MKLAINYIVKGTSYRLVINAEKLKVYEELDNVLKEICEKFNGEVEEYSVSKIVEELPLGKIIMPLATNVTLHSSDIKFSDDDNLNFIIKTLLGTTIGLVAGHLIDSFISREEKVIDIKNPCERKEPSIFQAITEWKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C3</accession>
    <name>F4B6C3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger C2H2-type domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C3"/>
    <dbReference type="STRING" id="933801.Ahos_1665"/>
    <dbReference type="KEGG" id="aho:Ahos_1665"/>
    <dbReference type="eggNOG" id="arCOG11402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2646084_0_0_2"/>
    <dbReference type="OrthoDB" id="36578at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="77" mass="9077" checksum="51BDCAF9714A5DA3" modified="2011-06-28" version="1">MAKRVLLKCELCGQVFASNSLYYQHKVLQHSDYKPIVKEDGYECPICHEKRKRLEPMLTHMGLQHLINNPIRIEIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C7</accession>
    <name>F4B6C7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CBS domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1669</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776462.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C7"/>
    <dbReference type="STRING" id="933801.Ahos_1669"/>
    <dbReference type="GeneID" id="10601169"/>
    <dbReference type="KEGG" id="aho:Ahos_1669"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867680_0_0_2"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="125"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="320" mass="33954" checksum="8C5A71157C1B559A" modified="2011-06-28" version="1">MLKAIMSSNLKTLDVNATLKDAAELMVSSGIRRVPVTVAGNIIGVVSARTVVKEAINNQNWATEKLSDIAKPAITVDPDTPFRTAAKLMLKYGVGSLIVKGQGIVTERDLAKVIPRVTIPAISVGTTNVLTLPSDSTVLDAAKSMISLGISHIPITSGSDVIGVVSLRDVLKAIHDGNTTGKLSDIASKSLVYEDIDASVEDIADLIVSKYVGAVPLFEGEPKAANLRGIITEWDLVRLYATMVRAHVLIKAEPSKIRGLVAALMATPRVYDVAIVYGPYDLLVTIDVEDPDLIGTTVINSIASMTGVKETTTLIEAEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F1</accession>
    <name>F4B6F1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776486.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F1"/>
    <dbReference type="STRING" id="933801.Ahos_1694"/>
    <dbReference type="GeneID" id="10601194"/>
    <dbReference type="KEGG" id="aho:Ahos_1694"/>
    <dbReference type="eggNOG" id="arCOG02235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020302_1_0_2"/>
    <dbReference type="OrthoDB" id="32867at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051691">
        <property type="entry name" value="Metab_Enz_Cyan_OpOx_G3PDH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42949:SF3">
        <property type="entry name" value="ANAEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="359" mass="39498" checksum="B6A9B1DF6CA3F99C" modified="2011-06-28" version="1">MAGEKVLVIGSGPAGLSATKELLNMGVDVVIVEKESYLGGTPKKLKYSLLFPELRPASEVIDPLIKSADGAKKYMESIVESAKPEGKGFVVTIKDKTGKTTTEKVSAIIAASGFEHFDSRRKYEYGYGIIPNIYQISDIERMLSENKLVTTKGTPPKRVAILLCVGSRDATVGNTYCSRVCCAVSIKQAMEIKQRIPDAVVHIYYMDIRTYGLMEDKLYWRSQLEYRVGFIRGRISEFMRGPNDTVIIKGEDTMNLNRALVVPYDMVILANGMELGLGSKQVAKALGLEFEEHGFVKPLDPDRLPVQSTRKGIFLAGAITGPKTIADSITEGQAAAMKAYEYITKGVWEEPVKVEVVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J1</accession>
    <name>F4B6J1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776526.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J1"/>
    <dbReference type="GeneID" id="71811310"/>
    <dbReference type="KEGG" id="aho:Ahos_1734"/>
    <dbReference type="eggNOG" id="arCOG03737">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438169_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="175" mass="20054" checksum="3F5C754CEB279FDF" modified="2011-06-28" version="1">MPSKDEIVKAILKVTGGDENKEFTKTAIAHELGMTKSTSSIDKIIKELESEGFIKFKEKRGPGIYYVLAKKQVVEEKNAEELHDMKNLKVALREVLEEYFGKPKSFADLDSTYDFMKDSLGYVRIDNLRRQLGMSLEQFMAKFGDYILQHYELIPGGEEGFIKGGVMYGIIRRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K8</accession>
    <name>F4B6K8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide 5'-hydroxyl-kinase</fullName>
            <ecNumber evidence="2">2.7.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0530</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-phospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.1.78" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775334.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K8"/>
    <dbReference type="STRING" id="933801.Ahos_0530"/>
    <dbReference type="GeneID" id="10599971"/>
    <dbReference type="KEGG" id="aho:Ahos_0530"/>
    <dbReference type="eggNOG" id="arCOG04127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051301_1_0_2"/>
    <dbReference type="OrthoDB" id="359472at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051734">
        <property type="term" value="F:ATP-dependent polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="10">
        <location>
            <begin position="85"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="343" mass="38899" checksum="E65090C1E9B5738A" modified="2011-06-28" version="1">MLVKKGIDYITKGPCKLTVVKGRILIKGIEINEKEYKQINEESFSIVPKEESEIDTDCQILAEIPHLGWEELASQMSGGKVLLLGSTDSGKSYFSDIIHNMNKDSVIIDADVGQSRYLPTFISSTSGILEFFGDVSPSRNYRLHIELIGKILDNEKHSLTIIDTDGWIRGYKAFLHKLYMIYELDPDFIVSFDDKIVDYFPSNIRKKVIIVKKIPPFLERSRAKRISYRISKYKKYFEKASIITIEYEQVLGKKLAEGLFVGFDEPLQLFHEEPCSGYFIEELLGGLVGIVNEGKIAGAGIIKEINQDGVKILTPVNKVQGAILGNISLNEEFKERRIRFSKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M3</accession>
    <name>F4B6M3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775349.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M3"/>
    <dbReference type="STRING" id="933801.Ahos_0545"/>
    <dbReference type="GeneID" id="10599988"/>
    <dbReference type="KEGG" id="aho:Ahos_0545"/>
    <dbReference type="eggNOG" id="arCOG04170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032279_1_1_2"/>
    <dbReference type="OrthoDB" id="10741at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="entry name" value="Glycerate kinase, MOFRL-like N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="entry name" value="MOFRL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053656">
        <property type="entry name" value="Glycerate_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041176">
        <property type="entry name" value="GlyK_Thmprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227:SF0">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82544">
        <property type="entry name" value="GckA/TtuD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MOFRL-associated" evidence="2">
        <location>
            <begin position="5"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="290"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="395" mass="43657" checksum="36766925C954B76C" modified="2011-06-28" version="1">MNFSIVDKILEYADPYNALMEKVKVENGKIIVQGKEYKFSKPLMIAVGKASYKMAKFFLDRIEPVDYVVITPHGSNLPLKNVIEAGHPQVDEFSLKAGKRVKEMLTTEDYDLLIFLLSGGASALLEDPVIPLEEYRKINDRLIKSGLEITKINIVRKHLSRLKGGSLARLSKAKVLTLIVSDVPGNDLGSIGSGYTVADKSTVNDAKEILEDIGLPEYSRYLIETPKEVNADNHIILDNMDVLKKISSHLYNPLILTSQVVGEAKDLGKFIASIYKSISQYSIPFKKGSIIIGGEPDVTITGKFGKGGRNGEVALSLLEEARGNYEFYAIATDGIDGNSEYAGCVIRGDMNISYEEIEESLKNHSSYELLEKYSAVIKTGLTYTNVNNVYILIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N7</accession>
    <name>F4B6N7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775363.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6N7"/>
    <dbReference type="STRING" id="933801.Ahos_0559"/>
    <dbReference type="GeneID" id="10600002"/>
    <dbReference type="KEGG" id="aho:Ahos_0559"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150444_0_0_2"/>
    <dbReference type="OrthoDB" id="32909at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053795">
        <property type="entry name" value="Lrs14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040939">
        <property type="entry name" value="trans_reg_lrs14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14160" checksum="9DA61433185CA76A" modified="2011-06-28" version="1">MEQSQISGSRIKLPSGKDAGLVDILSFCYGLSETDVQVLMALMKGDARGTEELESDLKLSKASINRSLNKLLEMALVMRIKEPGNKAGRPRYLYKAKDYNELKSKILQDIKDCSDKMASLVESEFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Q8</accession>
    <name>F4B6Q8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775384.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Q8"/>
    <dbReference type="GeneID" id="10600024"/>
    <dbReference type="KEGG" id="aho:Ahos_0580"/>
    <dbReference type="eggNOG" id="arCOG05989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166553_0_0_2"/>
    <dbReference type="OrthoDB" id="45877at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="13255" checksum="371B02106922EA1E" modified="2011-06-28" version="1">MKAIHNLSKEARQEIINILLEKRSRKELAEELGITPAAIVKFTKGLTHPSDDTILKALEIASDDEKEKIVNIILNDLVNSVIEVFSEYPNVTTEKIDELKKILDEIEGRKLLASSGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R0</accession>
    <name>F4B6R0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0582</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R0"/>
    <dbReference type="STRING" id="933801.Ahos_0582"/>
    <dbReference type="KEGG" id="aho:Ahos_0582"/>
    <dbReference type="eggNOG" id="arCOG00492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000053">
        <property type="entry name" value="Aminotransferase, class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF19">
        <property type="entry name" value="KYNURENINE_ALPHA-AMINOADIPATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="61"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="404" mass="45683" checksum="055FC5D58348006A" modified="2011-06-28" version="1">MLMAKWSKFLSKDTTLLKTSEIRDLLKLTEGKNVISLAGGLPDPSTFPAEDIKNITDYVLQNYASRALQYSATAGIPELRKELVNLSSQRGITGINENNIFVTVGSQEALFMLFNIFVDPGDHVFVEMPSYLAALNILRARNPQFHGIPLTEKGPDLDKFEEELRRLKSEGKSPKLLYIIPTAQNPGGTTLDMEGRKRVLELASQYDFLIVEDDAYGFLVFEGESPAPIKALDKEGRVIYTGTFSKILAPGFRLGWIVADEEIIQNVELYKQNVDLHTPSFTQFIAAEAIKRNVIQNNIPRVRQLYREKRDVMLQAIEEYFPKDARWSKPVGGMFVFAWLPEKIDALNMLPKALERGVAYVPGSSFYYDYSGRNTMRLNFSFPPKEKLVEGIRILGQVIKEELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T0</accession>
    <name>F4B6T0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein, Csa5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776538.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T0"/>
    <dbReference type="STRING" id="933801.Ahos_1746"/>
    <dbReference type="GeneID" id="10601246"/>
    <dbReference type="KEGG" id="aho:Ahos_1746"/>
    <dbReference type="eggNOG" id="arCOG03823">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811533_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010157">
        <property type="entry name" value="CRISPR-assoc_Cas5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01878">
        <property type="entry name" value="cas_Csa5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09702">
        <property type="entry name" value="Cas_Csa5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="15012" checksum="04B5B7CCC2DE81AF" modified="2011-06-28" version="1">MDEEKIEGIVRRIANLFATTYLYSESSMLVDRFANALSKEAVAKVLYDAQRIVQMGLDRDEIKSDIVKIKEKDYPAIVITKEKEKSVQIIGYLPTDQDVEDFLSLIEKDVYYARKAGALAMSIANRTKLGGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U0</accession>
    <name>F4B6U0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776548.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U0"/>
    <dbReference type="GeneID" id="10601256"/>
    <dbReference type="KEGG" id="aho:Ahos_1756"/>
    <dbReference type="eggNOG" id="arCOG05713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_671964_0_0_2"/>
    <dbReference type="OrthoDB" id="26009at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="403" mass="47937" checksum="DEF15971C787E382" modified="2011-06-28" version="1">MTKRDIELLNRVGKYYLQYKGVVDIEKLLSLFEGTTELLVNDYTLSYIYFYIIYLVTKDAVRSAREALKDRLTYKEFSVEYSQELFGELDVTRTIAVYPMNMLAYYNFAEGLNAPEYSILGYLLRRIYHLVKEKHESLKYESSNDIPKYFNFREDFKKKYEELSDLKEKFPEGYYREPSYTDPEWLIRAYKAYFLIKGLESIKVGTKEKGVVTDKKIIKFLFWKLYELYVFYLVMKYLKRKGYEIIRKDGEFFAKKGDKLIRLVFNTSLTTSSLRKVDNTENIDKYKGRPDISLDKLPKPIIFECKYSPSVSYITMGRFKVMAYTYEYDPEVAVLVYPGLKESQVDYDAEDSATRELDQMVKEKGFLLDFYYNSHLIYMAIIDPLREDRENLRIIDKILGKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B717</accession>
    <name>F4B717_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dCTP deaminase</fullName>
            <ecNumber evidence="3">3.5.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Deoxycytidine triphosphate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dcd</name>
        <name evidence="4" type="ordered locus">Ahos_0612</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of dCTP to dUTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dCTP + H2O + H(+) = dUTP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="EC" id="3.5.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dCTP deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775416.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B717"/>
    <dbReference type="STRING" id="933801.Ahos_0612"/>
    <dbReference type="GeneID" id="10600059"/>
    <dbReference type="GeneID" id="42780225"/>
    <dbReference type="KEGG" id="aho:Ahos_0612"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_3_0_2"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="97"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="178" mass="20359" checksum="00406C97B9660922" modified="2011-06-28" version="1">MILGDRDLKYYLEKGWIVISPLTQDTIRENGVDLRVGGEIARFKKTDEIYEDGKDPRSFYEIEKGDEFIIYPNEHVLLVTEEYVKLPNDVMAFVNLRSSFARLGLFVPPTIVDAGFEGQLTIEVLGSAFPVKIKRGTRFLHLIFARTLTPVENPYHGKYQGQQGVTLPKFKFQITSMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B726</accession>
    <name>F4B726_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="8">GSA</shortName>
            <ecNumber evidence="8">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="8">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hemL</name>
        <name evidence="10" type="ordered locus">Ahos_0621</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775425.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B726"/>
    <dbReference type="STRING" id="933801.Ahos_0621"/>
    <dbReference type="GeneID" id="10600068"/>
    <dbReference type="KEGG" id="aho:Ahos_0621"/>
    <dbReference type="eggNOG" id="arCOG00918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="OrthoDB" id="6524at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000021">
        <property type="entry name" value="Glutamate-1-semialdehyde 2,1-aminomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000818">
        <property type="entry name" value="PRK00062.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43713:SF3">
        <property type="entry name" value="GLUTAMATE-1-SEMIALDEHYDE 2,1-AMINOMUTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="416" mass="46210" checksum="54B5E35DA0423F44" modified="2011-06-28" version="1">MSSELWKEALRYFPGGVNSPVRAAVKPYPFYVSQGKGAYIYTEDGNSLIDYVLGYGPLILGHANEYVKEKVIEQIEKGWLFGIPSRVEIELARKISFHMPSIEKIRFVNSGTEATMNAIRLARGYTKRDKILKFNGNYHGAHDYALVDAGSAASEFGVPNSEGIPQEVLKTVIVCEYNDLQCVEKKLKNEDIAAVIVEPVMGNMGVIPPEKGFLKGLREFTSTYNTLLIFDEVITGFRLSMGGAQEYFGVSADLTTLGKIIGGGFPIGAFGGKREIMDMLTPSGKVFNAGTFNANPISMTAGLATLEVMERENVIEKTTYVAEKVTEELERIKYPHVINRVKNMFQIFFGVEKVSNATEARRAKKEVYLKFQVNLMKNGVYFPPSQFESVFTSLAHYDDNVISDTVLAIKKSSELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B757</accession>
    <name>F4B757_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isopentenyl phosphate kinase</fullName>
            <ecNumber evidence="2">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_0653</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775456.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B757"/>
    <dbReference type="STRING" id="933801.Ahos_0653"/>
    <dbReference type="GeneID" id="10600104"/>
    <dbReference type="KEGG" id="aho:Ahos_0653"/>
    <dbReference type="eggNOG" id="arCOG00860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070213_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="10">
        <location>
            <begin position="10"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="226" mass="25281" checksum="774D5BC6B4AFE0B0" modified="2011-06-28" version="1">MGLKLGFAYRIIKLGGSLITNKNIPYSFNEEIVRRIAREVKKFYPLIIIHGGGSFGHYEASKGDKIKTVIAMQELNLKIMKIFYDEGINVFPLPGRFFSIDIVKRYLQERLVPVIYGDITESGKIISGDDIAILLGKEFNSRVFFATDVDGVFINGRVAKEVNSPFIDDLSSSSFDVTGGMKAKIQKILENCIEALIFNGRIEGNIYKALEGKDIGTLVRCKNDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B761</accession>
    <name>F4B761_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="10">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpo10</name>
        <name evidence="10" type="synonym">rpoN</name>
        <name evidence="11" type="ordered locus">Ahos_0657</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the 13-subunit RNA polymerase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775460.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B761"/>
    <dbReference type="STRING" id="933801.Ahos_0657"/>
    <dbReference type="GeneID" id="10600108"/>
    <dbReference type="KEGG" id="aho:Ahos_0657"/>
    <dbReference type="eggNOG" id="arCOG04244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_1_1_2"/>
    <dbReference type="OrthoDB" id="371754at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000335">
        <property type="entry name" value="DNA-directed RNA polymerase subunit N, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="66" mass="7665" checksum="18F1925197D8B480" modified="2011-06-28" version="1">MIIPVRCFTCGSLIADKWEPFITRVNAGEDPQKVLDDLGVRRYCCRRMLLSHVDIIKEVVNYTRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B768</accession>
    <name>F4B768_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps13</name>
        <name evidence="10" type="ordered locus">Ahos_0664</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <text evidence="7">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
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        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
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    </dbReference>
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        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00416">
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="168" mass="18756" checksum="3D15E08EE6240D51" modified="2011-06-28" version="1">MSESFKYIVRLFGQDVDGTMKVPYALAMVKGIGYNTARAIVRQLGIDKDRRLGELSDSELKKIEEALSDKTIKGLPTWMLNRRKDYESGLDLHLVTSELIFYARNDIEREKKIKSWRGVRHSLGLKVRGQRTRTTGRTGTTVGVKRAKAAQPAGGGQAQSSQSSQAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7E2</accession>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7E2"/>
    <dbReference type="STRING" id="933801.Ahos_1889"/>
    <dbReference type="GeneID" id="10601388"/>
    <dbReference type="KEGG" id="aho:Ahos_1889"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_762112_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:transmembrane transporter activity"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR010645">
        <property type="entry name" value="MFS_4"/>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SUGAR TRANSPORTER"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
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        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
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    </feature>
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            <begin position="6"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="361" mass="40201" checksum="BED02EF87709D6DD" modified="2011-06-28" version="1">MQRIKIFAYSWIITFLQLLFRSAWGVISVPVAMQFHLTSVQIGLVLTSFYVGYVISSTPWGLFIDLHGVKYAVLISSLVSSIVILLIFLFPSYDIILFSYLLSGLIVSALFPSSVKIVSVKLSPLYFYLGMLESSAPFAVLLLGLISGLLYNYWRIFYLLLSVSLLSVFFITLSMKDDYVSGKRRETKVLFTRFILLSILVRSGEMWVSWGTTAWLFPFLVLYFHFCNSSLIFLLFSLGLVSSTVVSSKLPGLIGEKKVVQISLILYVLFLFLLFLTRVSWLSFFLGIFSFSFRSPTDTLIVKFAGKENSSSSIGLANTISQIGSLLAPISIGFAVKFSPLLGIFTLSAGALLSLAVSFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7E6</accession>
    <name>F4B7E6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7E6"/>
    <dbReference type="KEGG" id="aho:Ahos_1893"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075237_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="30428" checksum="1AAF333876B72611" modified="2011-06-28" version="1">MPMSFQTDVIALQKLKDASLWFIIIGVLALIGVVASVAAVVMAIVDLVLLFMMAIPKLRDAFQTFASTGKDVSRGFTGINLILPGIIIELIGGILAIAGIFSASLGLAAAGGIIVLLAAIVLFIAYLLIGLVIYNLGSFYNNDMLKIGGILIIIPFISFVGWILSYVSVDDIIRKLSGGTMMPQPYTAPSMYQPQVYQVGQGIIRLDGTVTLTLYSSITVNIVSASIDNTLYTTTDVNPNMLNPGNNSVTMRFLGVTGLVPGNNYYLTIYLSNGTSIKAVVTCTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F1</accession>
    <name>F4B7F1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase DnaG</fullName>
            <ecNumber evidence="10">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dnaG</name>
        <name evidence="12" type="ordered locus">Ahos_0669</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteropolymeric, adenine-rich transcripts and the exosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a ternary complex with MCM helicase and DNA. Component of the archaeal exosome complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775472.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F1"/>
    <dbReference type="STRING" id="933801.Ahos_0669"/>
    <dbReference type="GeneID" id="10600120"/>
    <dbReference type="KEGG" id="aho:Ahos_0669"/>
    <dbReference type="eggNOG" id="arCOG04281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="OrthoDB" id="8643at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050219">
        <property type="entry name" value="DnaG_primase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003108">
        <property type="entry name" value="PRK04031.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313:SF2">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110455">
        <property type="entry name" value="Toprim domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7 10" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 10" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="165"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="398" mass="44235" checksum="F31453C73ACE9317" modified="2011-06-28" version="1">MKYIIKLYFETEGIVDKPDVIGAIFGQTENLFGEEFDLRELQDKGRLGRIVVEMETKGGKTKGTIEIPSNLDRIETALIASMVESVEKIGPYPAKFELKEIQDIRAEKLKKIIDRAKEILTNWSKEKTLDIKEVLNEISSAVKTGEITEFGPEKLPAGPDVLTDPNLIIVEGRADIINLLRYGYRNTVAVEGASGRIPQSVIDLSKQKNTVIAFLDGDHGGDLILKELLNANVKIDYVARAPQGREVEELTGKEIAKALSNMVPISQYLKKQQETPQVIVKTPEETVTVIQPPKEVEIQIPPSALEEIKKLPGTLEGIMFDENWNPVEKVQVRDIIPKLEAMSENKVAYIVFDGVITQRLLELAASKKVKLLVGVRIGGINKSLDNVKILTMSDILTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F3</accession>
    <name>F4B7F3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein-alanine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775474.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F3"/>
    <dbReference type="STRING" id="933801.Ahos_0671"/>
    <dbReference type="GeneID" id="10600122"/>
    <dbReference type="KEGG" id="aho:Ahos_0671"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17787" checksum="74AAC1DF52BE96A5" modified="2011-06-28" version="1">MVIITDATEDDLPQIYEIEVESFDNPYPYSLLKAYLYIADGLYLVAKEDGKILGYVIGIIQFKIRGHIVSIAVRKNCRNKGIGKLLINEIERRFKLGHCKYSYLEVMTTNKDAFFFYVRNGYFPLFVRKNYYGRGKHAFIMIKDLYSRKGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F4</accession>
    <name>F4B7F4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pelA</name>
        <name evidence="11" type="ordered locus">Ahos_0672</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775475.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F4"/>
    <dbReference type="STRING" id="933801.Ahos_0672"/>
    <dbReference type="GeneID" id="10600123"/>
    <dbReference type="KEGG" id="aho:Ahos_0672"/>
    <dbReference type="eggNOG" id="arCOG01741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="OrthoDB" id="31300at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="338" mass="38681" checksum="2612D82E11905EF7" modified="2011-06-28" version="1">MKVLEFDEKKGTMKVHVEDEDDLWTLHMILNKGDKVIARTSRDVSMGNEGKRVSMIIELQVEYTEFQAFTTRLRIHGIILDAPERYSIKGAHHTINLDIGDEIIIIKEKWNKSVLDRIYKQAEKKNRVLIALVDFDEYLIAIPMIQGIKILTEKSLSTPTKEEGIIEDNAREVAKEIENYLNSYNNIDAILIAGPGPFKEIVRKYLNTKVKIYMDSVSSATEAGLNEVLKRDIIDQIMRDYEISQSEKDLDKALMLLNKDSGLIAYGIDETKKASEYGAVDSLLVIEDMVTENEEVQNIMEEVEKRGGKVHIIPRDSPIYFQVKNFSGILAILRFRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q6</accession>
    <name>F4B7Q6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1930"/>
    <dbReference type="eggNOG" id="arCOG11331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2911279_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="61" mass="6653" checksum="772F0C7B559724FC" modified="2011-06-28" version="1">MSGIMETFYINTELKNGNIAIAAMLFGAFYATSAVISQAVVLSQPMLLQSLLLLDLLRMTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X0</accession>
    <name>F4B7X0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0761</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775563.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X0"/>
    <dbReference type="STRING" id="933801.Ahos_0761"/>
    <dbReference type="GeneID" id="10600218"/>
    <dbReference type="KEGG" id="aho:Ahos_0761"/>
    <dbReference type="eggNOG" id="arCOG00933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023791_0_0_2"/>
    <dbReference type="OrthoDB" id="49555at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="GDGT/MA_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056488">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045702">
        <property type="entry name" value="rSAM_GDGT_ether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306:SF1">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23545">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01100">
        <property type="entry name" value="methyltransferase_(Class_D)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="133"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="582" mass="67886" checksum="5CBD0F7C5422387A" modified="2011-06-28" version="1">MAQVETQKKEGGFRLLPAPSKYENGVVKFGDREIKIGGPLPKLAENEKLIRVTHSLCPVCYRLLPAAIFEKDEKMYIRKVCPEHGEFEDLYYGDVGMYYKFDYWEYEGKGPKVPYVDLKSPCPFNCGLCPMHHQHSALVNLVITNRCDQSCWYCFFFAEKAGYVFEPTLEQIKFMVDQLKRQDITLVIQITGGEPTLREDLVQIVKLLRESGVKHIQLNTWGGTFARLYMEDPDKAIKYAMQLREAGVNTVYMSFDGTNRRTNPKNHWEIPYTLEVFRRAGMTSVVLVPTVIKTTNDNDLGNIVRFAAENIDVVRAINFQPVSLTGMMKRNMRSRFRITIPEIIKDIEDQTDGEITRDSWYPIGTSVVFSRLIEALTGKEQFEMANHPSCGAGTYVFVEWREGKPHFIPLSKFIDLEGLLEYFKEKAEELKEGGNKYWVGLKILYNIRKFIDKEKGPKDFDVYKMLYNIIVNHNYEALGEWHYRTLFLGTMHFMDLYNYDIQRVMRCDIHYVTPDGRVIPFCTYNVLNDLYRDKILKEYQISLDEWVKLHGENSIGDQVKYKRVVSDLEKGEIYKNTYKHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y5</accession>
    <name>F4B7Y5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775578.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y5"/>
    <dbReference type="STRING" id="933801.Ahos_0776"/>
    <dbReference type="GeneID" id="10600235"/>
    <dbReference type="KEGG" id="aho:Ahos_0776"/>
    <dbReference type="eggNOG" id="arCOG04309">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059734_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="entry name" value="Bacterial fluorinating enzyme like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="entry name" value="S-adenosyl-l-methionine hydroxide adenosyltransferase, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046470">
        <property type="entry name" value="SAM_HAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046469">
        <property type="entry name" value="SAM_HAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_OH_AdoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092:SF1">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20257">
        <property type="entry name" value="SAM_HAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_HAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="Bacterial fluorinating enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="Bacterial fluorinating enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase C-terminal" evidence="4">
        <location>
            <begin position="157"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="252" mass="28200" checksum="ADE26CD27CC9A414" modified="2011-06-28" version="1">MEAVIRRINNEVDITYITPNSKNFNLYAASYLLYTAYKYFRKGSIFLVVVDPGVGTSRKALLVKSKNYFFIGPDNGVLYPAISEDGIDEVIEISNPRLYLSKNISRTFHGRDIFATTAGFASAGVDIHTFGPKISKDELVKLSYNYNIIKDKNLVCGKIIYIDHFGNIATSIRNISFVDENVKIIFNNNRLIARKVNTFGESKGNEFLVYKNGYGFIEIGINKENASIKLNASEGEDICLEGSIQEDSSHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z0</accession>
    <name>F4B7Z0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="6">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="6">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tiaS</name>
        <name evidence="10" type="ordered locus">Ahos_0781</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + agmatine + ATP + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775583.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z0"/>
    <dbReference type="SMR" id="F4B7Z0"/>
    <dbReference type="STRING" id="933801.Ahos_0781"/>
    <dbReference type="GeneID" id="10600241"/>
    <dbReference type="KEGG" id="aho:Ahos_0781"/>
    <dbReference type="eggNOG" id="arCOG01115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="OrthoDB" id="39189at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="TiaS_FLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055394">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF1">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="TiaS_FLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23783">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TiaS-like TCKD" evidence="8">
        <location>
            <begin position="4"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS FLD" evidence="7">
        <location>
            <begin position="142"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS C-terminal zinc ribbon" evidence="9">
        <location>
            <begin position="349"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="432" mass="49415" checksum="2C4E5E30C1EFC743" modified="2011-06-28" version="1">MKYIIGIDDHDSPNRGCTTHFATNLIKIFHKDGIKLLDFPYLIRLNPNIPWKTRGNASIKLIVESDKDIEEIADIVWNASLDYVNNVSQGLKYGRKPGMAIVNYESSIILENFYTKAVSDVITQGFMEKYIQKTNAIVRGSRGIIGSLAAIGFEGNYTFELLTYRKEENWEKKREIDLDSLIAFDEKFFPKVFANVDYTKKRPLILSHGGDPVFYGIRGIDPEILLKGIDMIQIKDEEIENFMIFKSNQGTDAHIIKPGDKVYQTYYGKVTIKDVEIIPGGDVILRTENGLTIFVYKETGELNSMAKLLLSGDEIVVLGAIKPSSKYGRIIDAERIEILSLSNKVEYKNPKCPICGHSSESIGKNKGYRCKKCGYKFNSEKEIIEFPRDICLGTYQTRYYRHLTKPIFLEFYNDYKKETEVLNKILNILLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B809</accession>
    <name>F4B809_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="10" type="ordered locus">Ahos_0800</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775602.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B809"/>
    <dbReference type="STRING" id="933801.Ahos_0800"/>
    <dbReference type="GeneID" id="10600261"/>
    <dbReference type="KEGG" id="aho:Ahos_0800"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5005051038" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023462587" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="6"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="196" mass="21363" checksum="23DF267C95E0078A" modified="2011-06-28" version="1">MEELPSTAVGIKLNDGVILAAERRLSYGGYVLSKSAKKVHIIGRFGIAGAGLFGDLQALTRIMNAEIKYYELYNEKPISTKAAAKLLSIILYQYKYMPFISEILFAGVDNGIPQLYVLDPLGSLLDDVYAAVGSGARVAIGVLEAEYDQNMPLDKGKEIAIKSIKASIERDVTSGDGIDILILDKTGKTYTEYIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B823</accession>
    <name>F4B823_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776762.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B823"/>
    <dbReference type="STRING" id="933801.Ahos_1975"/>
    <dbReference type="GeneID" id="10601474"/>
    <dbReference type="KEGG" id="aho:Ahos_1975"/>
    <dbReference type="eggNOG" id="arCOG04032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053469_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="433" mass="50006" checksum="9012CE9D3EFFB09D" modified="2011-06-28" version="1">MDKEMMPSSLLAHYNKEIVKKEIFEFSKNRWIALYGKTMIRYNGEEPLSLSSPEEVPSLVKRFNARTIYATAGKYEKVNKENVSGDKIVAYTPFFDIDTKIDKWEYAIKAADVIISALDKEGVSMSVYLLWSGEGIHVRINENAIPKDYDPLTAAHAIVQYILRKVKDEIKKLSDESEGVLKIDELIDAKRIFTAPLSFHKELDYVAVCFSPDKLHEFNLDWAKPDNFRHEEGVFSRYVEDETEELLSKAILEYKPTHEGIKPERRTTPQGKIGRFQVMGIVQAARYYVLYGDLPKAESFGLNRAIFYAWAKYYGKGYTPKVTGQKLPAEFRKDRILVTVAGEQVYQNPENGLFVIGDKEQTPEDYNREIKEKINAVIPYDKAWEATVKYVSSFPKEVLLNQREFYQKVYLPIRDAFIEKVVNRKSGLDAFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B833</accession>
    <name>F4B833_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Radical SAM protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B833"/>
    <dbReference type="STRING" id="933801.Ahos_1985"/>
    <dbReference type="KEGG" id="aho:Ahos_1985"/>
    <dbReference type="eggNOG" id="arCOG01357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_743149_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="42520" checksum="5544DFB2B2D7E5F2" modified="2011-06-28" version="1">MILTADEATFYSLPPLPERILPIVRKNRYPPYGLRKIEAMTNSIILPPSKIRKPDILGIYVNDPLALTQPSLAIKEAFGENPRFYYSFQKFSAKIRELKSRFKFKVIVGGPGAWELVEKKEEDWIDTIVVGEAENVIHKAITEEGIIKGTPDEKFYPIKAPSAMAEVEVMRGNRKIPREVILSEMKIQSIQGKVNLISPDLFSYGDEEEIISLLKMSSEFGKVFFNQISLKGISKIDLRRVSRILNLNEKNYRTPVLSKEPGLCNVEVEGDEIKELNNNFIYPMIFVPTDSISSLEGYKALVIPLPSDGDNYYEALYKSWNISRKIIKNRIMISLIDKIILKNKETKGEFLRKLNVRSPIFLVQLFSLLLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B840</accession>
    <name>F4B840_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotidyltransferase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776779.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B840"/>
    <dbReference type="GeneID" id="10601491"/>
    <dbReference type="KEGG" id="aho:Ahos_1992"/>
    <dbReference type="eggNOG" id="arCOG01205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159724_0_0_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37030:SF1">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="113" mass="13548" checksum="5BD01DF79A7CAABC" modified="2011-06-28" version="1">MSFIDLLIGNAQNREKFLRNIDFYLNKIKEVVRNEDPNGRVILFGSYVRGNFRPDSDIDILVIMNVKGEEDRLRIYHEINEVVGRPNPFEIHVINDEEYRDWYSHFIDVYKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B858</accession>
    <name>F4B858_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalamin (Vitamin B12) biosynthesis CbiG protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2010</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B858"/>
    <dbReference type="STRING" id="933801.Ahos_2010"/>
    <dbReference type="KEGG" id="aho:Ahos_2010"/>
    <dbReference type="eggNOG" id="arCOG00651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028397_0_2_2"/>
    <dbReference type="OrthoDB" id="4722at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="entry name" value="CobE/GbiG C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048775">
        <property type="entry name" value="CbiG-like_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004467">
        <property type="entry name" value="PRK05788.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21437">
        <property type="entry name" value="CbiG-like_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="CobE/GbiG C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin synthesis G N-terminal" evidence="2">
        <location>
            <begin position="43"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="CbiG-like linker" evidence="3">
        <location>
            <begin position="127"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CobE/GbiG C-terminal" evidence="1">
        <location>
            <begin position="203"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="323" mass="35791" checksum="59E6784579B88BCD" modified="2011-06-28" version="1">MERAWRGFAIVYASKNNIAERLCEEMKKREVPCSLFSYKEADFENIWKCYDGIIFTMALSGAVRTVCKYAQSKDKDPAVIAIDDEGKFVIPILGAHWGANEYAEEIARLLDSTPVITTASELSNITSVEEFAKLINCKILNVENIVKITSALLRGEEVCVKGLKALPKVKGKYKIGDDCKYFVIIGENQGNNDNAVYLRPLKLSIGIGSKIETDERTIEEAILFALNKINADITQVEVISSVREKVRKVAEKLGVKFRLLSIDEVNSFKDECLSPQSEKLKELGIRSVAEACALISAGKNAKLILRKIPYKGEVTVSIASIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B860</accession>
    <name>F4B860_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Formate dehydrogenase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B860"/>
    <dbReference type="STRING" id="933801.Ahos_2012"/>
    <dbReference type="KEGG" id="aho:Ahos_2012"/>
    <dbReference type="eggNOG" id="arCOG01491">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_4_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.90">
        <property type="entry name" value="ADC-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.228.10">
        <property type="entry name" value="Dimethylsulfoxide Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF14">
        <property type="entry name" value="FORMATE DEHYDROGENASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="649" mass="72129" checksum="ABF7239EFC722293" modified="2011-06-28" version="1">MMIKSICPFCGVGCGIDLEVEEGKIVRVLPDRTHVVSKGHLCGKGSIAFKSLYAEDRVLYPLKKEGEKFVRISWKEAIDEIYFKLNKIIKEYGPFSVGFYGGCQNTLEEVYSFMKLARALGTNNVDSCARVCHDPSAIALKEMLGVGASSTSVVQIPKAKALVIAGESTTESHPVISQYIVELKRKGGKLIVIDPRLTGIAKIADLYLQISPSTDIYLFNAVANYLISNGLIDENFIEQRTEGFEDYKRVISKYSVDDAEKITGIPKEKIIEFAKLISQKPVIFSWGLGLTQSSGVNGVRALVNLALLTGNIGIEGGGVIVYRGQANVQGSGDLIKPNVFPNGKMDELHALQLKEVWGFKPPIQQGKTVTEALYGSNLKAVFLMNFNPAKSFPNRKVVEKFLSSLDLLVVIDSFMTETAKFAHYILPAAMWAEKEGSVTSLDRLVKWRFKAIDPLGEAKPELEIIADIARKFGFNFSSDPKEVFNELKEVSSYSNLNFGEITDYSFPSRYPQNDEVLYREKFLTEDGKAHFHPVEQPKLSKGMILITGREVTHYNTDELIVRSGFPEIPLEIFINPEDATSLGIKEGDEVEISSECGSAKARVKLSNEIMKGTVFAYMHNAEINYVVCNEMDEEAKTPKFKYTVVSIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B871</accession>
    <name>F4B871_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_2023"/>
    <dbReference type="KEGG" id="aho:Ahos_2023"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="216" mass="24496" checksum="CDEAD1F690C16D4E" modified="2011-06-28" version="1">MLSSNVFINAFYERGNFTIYGIILAFIPLISVSETIAFALALRNIIFVTGDHIYRGSIISFLTFPIKRTGLFLMMYFSDVITPYIIWLPTTEAYILMSGIPVPQFLILIYTIGYFFTENIILSIALTFKSAGIATLLSSFIIGLLFIFGGMLNYYELIQGYHVLGITSSLNPYVLLLYEAVNRKTLPCIYEGITIELIIATVTFFFSLNKFKRLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B892</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2045</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776831.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B892"/>
    <dbReference type="STRING" id="933801.Ahos_2045"/>
    <dbReference type="GeneID" id="10601545"/>
    <dbReference type="KEGG" id="aho:Ahos_2045"/>
    <dbReference type="eggNOG" id="arCOG05999">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551727_0_0_2"/>
    <dbReference type="OrthoDB" id="41808at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="172" mass="20309" checksum="7691804366059ACE" modified="2011-06-28" version="1">MIKYPLSTLALILNVVVALLPYCWWTYSVGGIIKIDDSLFLLNICFEGQYLYISNFIDMILFAFRFYVIAVSLYFLYLILRRGEKRNYLLIAWASLFYILDPVIIYIIFNYILSHFIQGVTYPFFIIGQESVEFTDSGYQITMLIQSYPTFTYWFSLAVGIINLISRFLKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8A8</accession>
    <name>F4B8A8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps3ae</name>
        <name evidence="4" type="ordered locus">Ahos_0821</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775623.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8A8"/>
    <dbReference type="STRING" id="933801.Ahos_0821"/>
    <dbReference type="GeneID" id="10600288"/>
    <dbReference type="KEGG" id="aho:Ahos_0821"/>
    <dbReference type="eggNOG" id="arCOG04186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="OrthoDB" id="30639at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_eS1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_eS1_arc"/>
    </dbReference>
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        <property type="entry name" value="PRK04057.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="208" mass="23766" checksum="6A29428410938BB4" modified="2011-06-28" version="1">MSSKSTSAIRDKWKLKKWYTILAPKVFGEVVLGSTPAFDVSYTIGRKVETTLYDLTGDFSMVYIHLYFKVVSHDGDKLITSFYGHELSRDYVRSLVRRKSSKINEITDVRTKDGYLLRVKGLALTTYRVHREQRTAIRKIMEDLIKKDAEGRTFDEFIQDMVFGKLANDIFNEAKKIAPLRKVEIEKSKLLEAPSQEVTVASSNPSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C8</accession>
    <name>F4B8C8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Isopropylmalate/isohomocitrate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0841</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C8"/>
    <dbReference type="STRING" id="933801.Ahos_0841"/>
    <dbReference type="KEGG" id="aho:Ahos_0841"/>
    <dbReference type="eggNOG" id="arCOG01163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000019">
        <property type="entry name" value="Homoisocitrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="345" mass="37797" checksum="3C459062FFE853D4" modified="2011-06-28" version="1">MNNVSLCMEYRIALLPGDGIGPEIVANSKKILSKLVENYALNLQITEVEAGDSALAKYGDPLPKQTLDIINKSHIILKGPVGESAMDVVVKLRQMYDMYANIRPAKSLPNVPNKYGNVDILIVRENTEDLYKGFEFMLSDDTSIGIKVITKKASERIVKVALKYALERNKKITCVHKANVMRITDGLFSRVCKEILKGSEVKFEEMYVDAAAANLVRDPSRFDVIVTTNVYGDILSDEAAQIAGSLGLAPSANIGDSKALFEPVHGAAFDIAGKNIANPTAFLLSVSMMLDYIYSLSKEIKYKHASNALQNAIYEVYKEGKTLTPDIGGSAKLTDFIDAIYSKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E0</accession>
    <name>F4B8E0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps17</name>
        <name evidence="7" type="ordered locus">Ahos_0853</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775655.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E0"/>
    <dbReference type="STRING" id="933801.Ahos_0853"/>
    <dbReference type="GeneID" id="10600321"/>
    <dbReference type="KEGG" id="aho:Ahos_0853"/>
    <dbReference type="eggNOG" id="arCOG04096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="OrthoDB" id="10698at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_uS17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_uS17_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="111" mass="12647" checksum="D4CD739604B36D2B" modified="2011-06-28" version="1">MSQAKNVGIVGVKAPEKACNDKNCPFHGNLRVRGMIFEGKLIKFRANKTGVFERVYLYYNSKYKRYERRRSRIRVHIPPCLDVKEGDNVIIGECRPIAKSVSFVVLGKVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F8</accession>
    <name>F4B8F8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytidylate kinase</fullName>
            <shortName evidence="10">CK</shortName>
            <ecNumber evidence="10">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytidine monophosphate kinase</fullName>
            <shortName evidence="10">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cmk</name>
        <name evidence="11" type="ordered locus">Ahos_0871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775673.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F8"/>
    <dbReference type="STRING" id="933801.Ahos_0871"/>
    <dbReference type="GeneID" id="10600339"/>
    <dbReference type="GeneID" id="42780513"/>
    <dbReference type="KEGG" id="aho:Ahos_0871"/>
    <dbReference type="eggNOG" id="arCOG01037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="OrthoDB" id="31096at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13189">
        <property type="entry name" value="Cytidylate_kin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="20105" checksum="5F43636DCF20A93F" modified="2011-06-28" version="1">MIIVISGPSGSGKTTVAKILSERLKLRFVSAGQLFREMAASLGKDIIEMNKSAEKKFDIDKLIDEKILDEAKKGNVIIESHIAGWLLSGIADYTIYLWANIEERAKRISIRDKISYEEALIKIMEREQSHYFRFWKYYGIDLLDLTPFDMVINTSNIDINRIVNIILAYIGKNTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H1</accession>
    <name>F4B8H1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="GeneID" id="10601552"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="195" mass="22649" checksum="C64E9C0D8C2036B1" modified="2011-06-28" version="1">MLDFEDIKIDIEDEKTREEILNFFSIFVYLSNFYEKNCIIHVHCNGVQIEGKPGFIIVSNFPKIYSKGADKLRALAYLSLFREETPIVENDIQAISSISLVPLYFSVKMKEKGIIKFLSSLTQYYSTIYNQLKNEKIREDFWKVLNSFKVIDTTFLENIKNSYLTHLELYAKLLAFASGSSLSYNDLLKKYGIIS</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13129" checksum="0C0957A981CBDF4B" modified="2011-06-28" version="1">MDKVDILLKEAEIDIKNGCYNKAVSASYFAVRMEIEKLAKKLRTPIPKRDDKLINILKHMGKDELAKESLYLYERRKDADYSYESLKEDLAIKCLELSKYLVQEIRKLTQSL</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AEE94953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="483" mass="52988" checksum="DB3EAD5DF282AB3C" modified="2011-06-28" version="1">MTKRAIILLAVIVAGIIIATQLFPIANVIFTSLFPGHDITLIIAGKRGLIRGFKVCAYMFYPTPQGTKFIKVFNSTSSSCTISIPLKNLLTPAKAWYSLYSYHIMPSLLGAVIHVKQCGNKVKIITQPFAIPINVSNVINCKGFTYTIMFNNPIVKTIEVKPECTTTVTYTPEGCGAIIVTPHILGWYPNSLSCKPIAIATFVGPLEIRCYVAGMCAIIASESSIGFYISQPNLKYNLIRVTIPGPSITLDNVNFQIKSSASYGIGKADYCNAFQIYIKGQVAWVNWTVCYENPRDYSHPFQVICYYYQVMLTRIVACKNGCAVAPKLYVYATHINICCSSSGGGLCHPKLESNLASIFNLSKYFPAPVNFTKLVKLACLSYCKCYSNNSLDLGHYLFGIGINVGALVAIASTNDSDLAPLVAPLSTVNIAVECSASLIVWEILNQYPEANLAFYYSNLTTEYCINGVLYQLPTGVFIANYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L4</accession>
    <name>F4B8L4_ACIHW</name>
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        <submittedName>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L4"/>
    <dbReference type="STRING" id="933801.Ahos_2095"/>
    <dbReference type="GeneID" id="10601595"/>
    <dbReference type="KEGG" id="aho:Ahos_2095"/>
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    <dbReference type="GO" id="GO:0016779">
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        <location>
            <begin position="23"/>
            <end position="102"/>
        </location>
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    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE94966.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
    <sequence length="104" mass="12310" checksum="347CBD93E7983D4C" modified="2011-06-28" version="1">MEQPIELLRNWRKLISNLSLDEYSEVYVFGSVVEGKITGYSDIDVLVIIPNKKDKLQYLLKFYDEVEKKLGEKISYLFDVKVIYPEEKSVPPYSWFLKKSIKIK</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="AlphaFoldDB" id="F4B8M0"/>
    <dbReference type="STRING" id="933801.Ahos_2101"/>
    <dbReference type="GeneID" id="10601601"/>
    <dbReference type="KEGG" id="aho:Ahos_2101"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173010_0_0_2"/>
    <dbReference type="OrthoDB" id="42978at2157"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9417" checksum="E5888D91EE52F0D4" modified="2011-06-28" version="1">MIGEILVKKADDKGRILISSFKGKDVYIIDLGSGYFITDNKEVAEAVSRHASKAFSDEFLKLVEELSPEEVEEIVEKEVVNKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8N3</accession>
    <name>F4B8N3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, CopG family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776900.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8N3"/>
    <dbReference type="STRING" id="933801.Ahos_2114"/>
    <dbReference type="GeneID" id="10601614"/>
    <dbReference type="KEGG" id="aho:Ahos_2114"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2679103_0_0_2"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="69" mass="7857" checksum="71D101D5D7409A96" modified="2011-06-28" version="1">MEQVKKVGDGVYEVEMNETLTISFKLEEELLKQVDEAVKSLGYANRSELIRDAILEYISYLEGKKNGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8P4</accession>
    <name>F4B8P4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cation transport ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Ahos_2125</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776911.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8P4"/>
    <dbReference type="STRING" id="933801.Ahos_2125"/>
    <dbReference type="GeneID" id="10601626"/>
    <dbReference type="KEGG" id="aho:Ahos_2125"/>
    <dbReference type="eggNOG" id="arCOG01578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_6_4_2"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000042">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="639"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="704"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="735"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="764"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="13">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="794" mass="88825" checksum="41CDC03E3A4D689B" modified="2011-06-28" version="1">MASNFEKMSIEETLTQLNTSLKGLTEKEAEERIKKYGYNEVKEKKESPIVKFLKKFWAPVPWMLEVTIVITYILGKYLDMYIIIFLLVFNSIVSFIQERRAENAVELLKQKLNVKARVLRDGQWKVIPARLLVPGDVVHIRLGDIVPADIKLFEGEVLVDQSALTGESVPVEKGKGNVVYSGSIIRRGEASGIVIATGAKTYFGKTTELVQTAKAESHLEKLIMNIVKYLIIVDVALVIALFVFSLAVGVKLSDVLPFSLIVLIASVPVALPATFTIAMALGSQELARKGILVTRLTASEDAASMDVLNLDKTGTITENRMRVGDPIPAEGFTKEEVVKYAYMASDEASQDPIDTAVIACLRDNNIAPGNYERLEFKPFDPSTKRTEALVKTENNVFRVVKGAPQVIAELAEVPDLKNYYSTLEELSKRGYRTISVAIGDKEGKLKLVGILPLYDRPRKDSREFIEEIKRLNVKPKMVTGDNELIAREIARQVDIGDVICNINEIKKLEGKERIKKVEECDVFAEVFPEDKYFIVKTLQEGGHYVGMTGDGVNDAPALKQAEVGIAVANATDVAKASASMVLTHEGLTDIVEAIKTGRRIYQRMLTYTINKIIKTLQVVLFLTLSFFIVRFFVTTPFDVILLLFANDFVTMSIATDNVRYSQKPERLDAGKIVKASLILAFLVVAESFFSLWLALYLRMSINEIHTFIFDMLVFTGQFTVYMVRERRSMWSSRPSNFLLISSIFDIIFISTISVLGILVYPIPLQYVLLILGVSFGFTVIFDRIKNISFKTVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8P7</accession>
    <name>F4B8P7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776914.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8P7"/>
    <dbReference type="STRING" id="933801.Ahos_2128"/>
    <dbReference type="GeneID" id="10601629"/>
    <dbReference type="KEGG" id="aho:Ahos_2128"/>
    <dbReference type="HOGENOM" id="CLU_1444602_0_0_2"/>
    <dbReference type="OrthoDB" id="376668at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="187" mass="21159" checksum="D6B07A4F5EA0BADD" modified="2011-06-28" version="1">MKIEDAYKEFITRLQLILAVIVITIVGYVISLFVDTTPLSLLSNFIVGLTLSYSLVASLAGYLYSPRFIDQIDKIREYFPQSTALGIILGFFFLLFSYLSTYIGFLSFFLDGLALAFDVLLTPLIFRGISFPKFMKEIKVGIKSDFTSFLILYVLALLSLLPLIDIIAIPLNAILSYLLLKEFYPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X0</accession>
    <name>F4B8X0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3311 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8X0"/>
    <dbReference type="STRING" id="933801.Ahos_0954"/>
    <dbReference type="KEGG" id="aho:Ahos_0954"/>
    <dbReference type="eggNOG" id="arCOG03694">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183045_5_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="8228" checksum="15429CB8DED2D41D" modified="2011-06-28" version="1">MHLFAMKKGFYLSLGIVLLVDIIIYSLYPLFNNVQPTLFGLTEFYWIQIVLLIVTSLLYFAIGYAFRGEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Y5</accession>
    <name>F4B8Y5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fuselloviral-related integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Y5"/>
    <dbReference type="KEGG" id="aho:Ahos_2137"/>
    <dbReference type="eggNOG" id="arCOG01244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083804_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031857">
        <property type="entry name" value="Integrase_SSV1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16795">
        <property type="entry name" value="Phage_integr_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase SSV1 C-terminal" evidence="2">
        <location>
            <begin position="116"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="271" mass="31848" checksum="33B3715981245798" modified="2011-06-28" version="1">MRGAGFEPARAYASGASVRPMGDTPIPPDLKNKGVITKDMEITRDKLNEFFEWCVNKRKNSIDTCKDYISYLKRPLNKNKKWSIFAYRLYYEFLGKEDKAKELKVEKKMSIPVYRIPSIEEIKKVLNHEDERIRILYRLLLESGIRLKEALFILNNYDPALDQMEDGFYVYTVNLIRKSKKSFYAFHITPLQKTYITESIIDHTDLPVKPKFIRKFVATKMLELGIPSEVVDFFQGRTPSSILSKHYLDLLTLAKKEYKKYAEWLSKMEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B994</accession>
    <name>F4B994_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SirA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B994"/>
    <dbReference type="STRING" id="933801.Ahos_1001"/>
    <dbReference type="KEGG" id="aho:Ahos_1001"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662099_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF6">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="75" mass="8286" checksum="13087A771F9341F7" modified="2011-06-28" version="1">MVKIYKKVDLTGTSCAGPIGELMGLMDEINRGEAVEALLKDEETKNMVVNWAKTVGLKIFEERKEGNNFVVVVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A4</accession>
    <name>F4B9A4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775813.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A4"/>
    <dbReference type="STRING" id="933801.Ahos_1011"/>
    <dbReference type="GeneID" id="10600485"/>
    <dbReference type="KEGG" id="aho:Ahos_1011"/>
    <dbReference type="eggNOG" id="arCOG03855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590775_0_0_2"/>
    <dbReference type="OrthoDB" id="41600at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="167" mass="18411" checksum="188D0E161DB75167" modified="2011-06-28" version="1">MESLSSFILVGATIIIGVIIFSLFASYGAIYSTNTLEVQEAQYYSSGLRISSGSPAGNQIPVIINDFNYPVGCLYLIAFLIKPCYSKSASTVTPLCYALINSTVPTKMTLTVLGLSGNIIYEGEIETYKVYVGNIEFVCTKPGYYTELWVVMKIDCQFFRIGYYVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C8</accession>
    <name>F4B9C8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cobalt transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1035</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C8"/>
    <dbReference type="STRING" id="933801.Ahos_1035"/>
    <dbReference type="GeneID" id="10600510"/>
    <dbReference type="KEGG" id="aho:Ahos_1035"/>
    <dbReference type="eggNOG" id="arCOG02250">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_2_0_2"/>
    <dbReference type="OrthoDB" id="43252at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34139" checksum="D6955750A0A35FF1" modified="2011-06-28" version="1">MIYIQYLLLPVVYFVTIFLMIKLGYGGLAEIVKYERRKIVVNPLSKILFEIGLFVFLTRGFYTILQLHILILAILFPFLLLSTIFLILRNKIKYLAYYVGAYLLITAWVEINSFYYYGNIIYTWPREIARIFGYSTVVTCSSLFHGILFSLYSPTLYITLLGFVFIATTNTSEVIRAFSELKIPLAITLIFSVFVKVIPQALQNMETSYKMTILRGLGYGKPLPIRLFYQLYALIAVILPVFVYIIKGSRNLAIALETRGYMSKKNRTSLVKIGFHLIDYLLLFISIIFFYISFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C9</accession>
    <name>F4B9C9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775838.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C9"/>
    <dbReference type="STRING" id="933801.Ahos_1036"/>
    <dbReference type="GeneID" id="10600511"/>
    <dbReference type="KEGG" id="aho:Ahos_1036"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17321">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF46">
        <property type="entry name" value="BLR6921 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="8"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="558" mass="59757" checksum="30442432E2D2DACB" modified="2011-06-28" version="1">MNRKKLLALTNTSIGIFMAFANYNMIIIALPAIFKGLNFNPTSPDSLGYLIWVILGYMVVTASLVVTFGRISDLKGRARLYTIGFLIFAIASTLLASITSTGNLGVMEMIIFRLLQGVGGGLLMVNSTAVLTDYFDKRELGKALGLNQVAGLVGGVAGLIIGGILSIYNWRFIFIFSAVVGIIGTIWSALTLKDIQKPIKRSIDVIGNTLFALGITILLISVTYGLLPYNSQPLGWSNPWVIAGILVSVGLISSFVFIERKVKDPMFDLSLFKNKDFSTSNTANMIASLARQGILLMLLVLLQGIWLPLHGVPYCDTPFWAGIYLIPNVLGFAVFGPISGILSDRYGGKILTSIGLTISGIGFLLLTLLPYNFNLYEFFAITFIQGAGMGLFTAPNTADMMASVPINKRGSASGMRAALQNTASAFSVVFYFSILISAMTGSLDSSISSALSSYGIHVNASIPASVVIFSALLGYDPLSAFSSSLPHSIAVKIDNPQFFVATIAPSFMSSLRLTLYISAGLLFMSALISAFRESGRVGRLGNNTQGEQKAERAIAKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F7</accession>
    <name>F4B9F7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAM-dependent methyltransferase TRM5/TYW2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777018.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F7"/>
    <dbReference type="STRING" id="933801.Ahos_2232"/>
    <dbReference type="GeneID" id="10601737"/>
    <dbReference type="KEGG" id="aho:Ahos_2232"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_0_2"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047729">
        <property type="entry name" value="tRNAMtaseTaw21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="6">
        <location>
            <begin position="7"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="248" mass="29010" checksum="A824CE5A4D8280EC" modified="2011-06-28" version="1">MEKWKRIEIVGDIAIIGIPFGKKPEDLKDYAKEIMSKHPYIKSVWGRYRDTKGDFRLPTYYHVLGEKRSETIYKEHGCKYYLDFTKVFFSSKLSFEHLRIAKEVKKGEIIINMFAGYGPFSILSAKLGKPKLVYSIDINPYAYYYMMANIDLNKTYNVIPIYGDTFKKIYYLENADRIISPLPEKAKEAYEIALQKIKPGGIIHLFVEIESKEDPVKEAMKLYPKAFFGRIVRSVKPFRYHVILDIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G7</accession>
    <name>F4B9G7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Sulfate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777028.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G7"/>
    <dbReference type="STRING" id="933801.Ahos_2242"/>
    <dbReference type="GeneID" id="10601748"/>
    <dbReference type="KEGG" id="aho:Ahos_2242"/>
    <dbReference type="eggNOG" id="arCOG09701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065753_0_0_2"/>
    <dbReference type="OrthoDB" id="41324at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="244" mass="27813" checksum="6DD083E25FC5B57A" modified="2011-06-28" version="1">MDLTLQLFDVLEDIWGDTSGIKMSFAGNQWFAAKDVLDFLKSKGYKVYLETIPPGMVRKRAEGESLKVGNLYISFTPEIVSLPPSLLEGLKIKKKVEYAENDIVIVFRGAEINNLCDLKGRRVAIPNPSIEGIGQLFKELYEEECGDYEDLINYGGVYITKVHHREIPHMLKLGDIDAGIMWRTEAIYWTFKYSVPQHNKKGKLAFALLDNASEKAEEVFNLLTSTEVKQIYEKYGFKWIADNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H9</accession>
    <name>F4B9H9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Conserved transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2254</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777040.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H9"/>
    <dbReference type="STRING" id="933801.Ahos_2254"/>
    <dbReference type="GeneID" id="10601760"/>
    <dbReference type="KEGG" id="aho:Ahos_2254"/>
    <dbReference type="eggNOG" id="arCOG01096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038957_2_1_2"/>
    <dbReference type="OrthoDB" id="42847at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="248" mass="27514" checksum="B5A9071019036C9E" modified="2011-06-28" version="1">MEKEIKEPVKHYTNEADTFRTKVFGIQDGLIGIGSIAIGAAGFSQEPLLVVITGLIATIGQAFSMGIGEYISTRVRMQVIQNEIKKEKYEIDNFPEKERDELIGFYIKKGLSREESEKIADIFMKNKETVLREMMLNELKITPEEFENPVKLGFLMSLYLIIGGLLPLIPFIISLFIRIPFYTSLLSSMTIMMASLGIFGSMATKYTGLSKLRGAIEQIGTGLIALIGSYVAGLLLAHFITIPPGLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I0</accession>
    <name>F4B9I0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0282 protein Ahos_2255</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0282 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777041.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I0"/>
    <dbReference type="STRING" id="933801.Ahos_2255"/>
    <dbReference type="GeneID" id="10601761"/>
    <dbReference type="KEGG" id="aho:Ahos_2255"/>
    <dbReference type="eggNOG" id="arCOG00969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079268_0_0_2"/>
    <dbReference type="OrthoDB" id="21331at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01406">
        <property type="entry name" value="UPF0282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014426">
        <property type="entry name" value="UPF0282_hydrls"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003287">
        <property type="entry name" value="PRK04286.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF4">
        <property type="entry name" value="UPF0282 PROTEIN MJ1629"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004944">
        <property type="entry name" value="UCP004944_hydrls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="308" mass="34799" checksum="2081E96ADD6A836A" modified="2011-06-28" version="1">MEIKPLAFESLGVRSQATFIETKDVRILVDPAVSLAPRRYGLPPHQREVDRLTELAKVITEKAREADIIIVTHYHYDHHDPGFIIPKDIYKNKKVFIKDPKENINMSQKRVRAPRFLNSIHGLPESIDTAEGKEIQIGSTKIIFSQAVPHGADERLGYVVQVAIKDGDQTVLITSDIEGAPKDQHLEFTLKVKPNVIIIDGPLSYLLGYALKQEDLDNSIKNLERVVREGAETMIVDHHVLRDLNYKTVLSNVYEIARSVNAKVITAAEFLNVEPLILEARRRELFKEENKPARIPRNLAQLLTAGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I3</accession>
    <name>F4B9I3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD-superfamily hydrolase, subfamily IA, variant 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I3"/>
    <dbReference type="STRING" id="933801.Ahos_2258"/>
    <dbReference type="KEGG" id="aho:Ahos_2258"/>
    <dbReference type="eggNOG" id="arCOG02293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050155">
        <property type="entry name" value="HAD-like_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43434:SF1">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="185" mass="20967" checksum="CE819F0DA6A830C9" modified="2011-06-28" version="1">MAWELALKDLGIKIPVDLDKLMGRKTEEIAKLLAKDRWKELYERKNAYFDNLVKIYAKPTPCAIDLVNKLKKEKVKTAVVTSSLRRSAIESLKILNFEPDILVAGDDVKKGKPDPEPVLKALSLLGEDPRNVFGVGDTLQDVIAYYNSGIREIYLVKGDLKIDYDEAMRKYNAKVIETLCCIINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L2</accession>
    <name>F4B9L2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775844.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L2"/>
    <dbReference type="STRING" id="933801.Ahos_1042"/>
    <dbReference type="GeneID" id="10600517"/>
    <dbReference type="KEGG" id="aho:Ahos_1042"/>
    <dbReference type="eggNOG" id="arCOG01097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095256_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052753">
        <property type="entry name" value="Rbr2/Nigerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33746">
        <property type="entry name" value="RUBRERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33746:SF4">
        <property type="entry name" value="RUBRERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="10"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="151" mass="17132" checksum="F5016C7193905772" modified="2011-06-28" version="1">MLRYFSDKMDLKGTKTAENLKTGFIGESMANRRYLYFAKKADEEGYPDIAAVFRSIAEGETAHAFGHLDFIRQGGLTDPATDKPIKTLEDMIQSAIAGETYEFTQMYPGFAKQAREEGFEEVAEWFETLARAEKSHAERYKQLLALLQGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M2</accession>
    <name>F4B9M2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Ribokinase</fullName>
            <shortName evidence="12">RK</shortName>
            <ecNumber evidence="2 12">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rbsK</name>
        <name evidence="15" type="ordered locus">Ahos_1054</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose + ATP = D-ribose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="2 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B9M2"/>
    <dbReference type="STRING" id="933801.Ahos_1054"/>
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    <dbReference type="KEGG" id="aho:Ahos_1054"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_2"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ribokinase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
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        <property type="entry name" value="PfkB_dom"/>
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        <property type="entry name" value="Ribo/fructo_kinase"/>
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        <property type="entry name" value="Ribokinase"/>
    </dbReference>
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        <property type="entry name" value="Ribokinase-like"/>
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        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="14">
        <location>
            <begin position="2"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    <feature type="binding site" evidence="12">
        <location>
            <begin position="203"/>
            <end position="208"/>
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        <ligand>
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    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
        <ligand>
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    <feature type="binding site" evidence="12">
        <location>
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        </location>
        <ligand>
            <name>substrate</name>
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        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
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        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
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        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE93938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="301" mass="33372" checksum="78DBA5B3443F5517" modified="2011-06-28" version="1">MIYVVGSYNTDYIIRVEEFPAMGETIFAKEIIVSHGGKGSNQAVSAARLGSKVRLIAAVGNDERGREALKFWKEEGVDTSGVKIKNTYTGSAYILVNRRGETMIVVNRGANYELNEDDVELDDGILLTQMEIRENVVKKALQRFEGIKILNPAPANISDYSILNYVDILTPNEIEFKELTSADDIEYGLNILLKKVKMAVIVTLGERGALIATKDKRVLISTPKVKAIDTTGAGDVFNAALAHSLEKGEDLESAVEFANIIASYSVTKIGAIGPKWEEVREFVEKEKRKEKGRRGEEERRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M4</accession>
    <name>F4B9M4_ACIHW</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="3" type="ordered locus">Ahos_1056</name>
    </gene>
    <organism evidence="3 4">
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        <lineage>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="aho:Ahos_1056"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1017881_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="204"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="273" mass="30861" checksum="C429D13903B53134" modified="2011-06-28" version="1">MPEELVEAQIVDFGKLKEIYSDILYYENYKSILKKTGGIDPPPPQSLRALPFSGRRIGPLSLIITGNMIKIKEIGAIVKPTKEGNPVYAIVDFSQGIKVLLAYEEESPIVGIDLGIRHLFTIVAIINHGKLYKVKYVGDKKMLDIFSKYLGESQGLVYLNDIRERVRKPVIETVKFLEELKPKVIAIEDLRLYEAKVGKGLRAIQEILEEELFKRGMKVRRLDPRGTSKICSRCGYKKGEVLGSIFVCPSCGYKADRDFNAAYNLALKCYYTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N3</accession>
    <name>F4B9N3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbon-monoxide dehydrogenase (Acceptor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1065</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N3"/>
    <dbReference type="STRING" id="933801.Ahos_1065"/>
    <dbReference type="GeneID" id="10600539"/>
    <dbReference type="KEGG" id="aho:Ahos_1065"/>
    <dbReference type="eggNOG" id="arCOG01167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053554">
        <property type="entry name" value="Glyceraldehyde_dh-related"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041018">
        <property type="entry name" value="glyceraldDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908:SF132">
        <property type="entry name" value="ALDEHYDE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20256">
        <property type="entry name" value="MoCoBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde oxidase/xanthine dehydrogenase a/b hammerhead" evidence="3">
        <location>
            <begin position="18"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="708" mass="77928" checksum="635470379B593BC1" modified="2011-06-28" version="1">MQYIGKPIRRIEDPRLISGKGSYVDDIKLPGEMFVAFLRSTKPHAKIHVKKTDNIFTGDDINPGKDFPIASRETTYVGQPIAAVIAKDRYEAYDLLESIEVEYEDLPYVLDPAEAIKDEVKVYSGLKSNIYFQKTFSSGNPEKILSESLTLEGELINQRVIASPIEPRGILAYFDGQRLNVWASTQSAHFMRRNLVEFLGFTNIRVIQPDVGGAFGSKIITHPEQYAVAKLAMKLGVPLKWVPTRTEEMISAGHGRDKRFKFKVGFTRDGKITALVGTIIGDLGAPYADANDDESGNVLSTSRMILGPYKIRDALITSYAVHTNKVPTTSYRGAGRPEATYFIERIVNMIANELNMDPIDIRLKNVIRPEEMPYESAFGITYDSGNYVEILNKAREYYNELKSEAGKDECVGLAMYVEITAFGPWEVARVFAKSDGKIIAITGSGPHGQGDATGFAQIVAETLQIPIENVEVRWGDTDIIEDGIGTWGSRTITVGGSAMLKASEELKRRLIEAAAKMLQADVEEIDYSEGKFTNKKSGKSATISEVISFAYKNGISLDVTYVYPVSKPTTPYGVHMALVSVDKELGKIKVKKYIAIDDIGKVINPLTAEGQIHGGVMQGIAQALYEEAIIDPQGNLVNSNLDDYAFPTAVESPKYTWTYIERGLSNHPTGSKGVGEAGAVVSTPVIVNAVENCLGKRIYKIPIKPDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N9</accession>
    <name>F4B9N9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GMP synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="3">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_1071</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775871.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N9"/>
    <dbReference type="STRING" id="933801.Ahos_1071"/>
    <dbReference type="GeneID" id="10600545"/>
    <dbReference type="KEGG" id="aho:Ahos_1071"/>
    <dbReference type="eggNOG" id="arCOG00085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="OrthoDB" id="33844at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="12">
        <location>
            <begin position="2"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="371" mass="42118" checksum="99E41EECC6A2DF5C" modified="2011-06-28" version="1">MFNPESFIEEVKPQILKEVREEKILAAISGGVDSTTAAVLMYKILGKQLLPIMIDTGFLRKDEARKVKDMLKDILPLQIVDKSEEFISALEGISDAEEKRKKFRDLFYSTLSAIAKEHGIRYLVQGTIAADWVETQGGIKTQHNVLVQLGIDTEKTWGFKVIEPLADLYKDEVRALARYLGLPKEISERQPFPGPGLLIRVVGKLTREKLEIEREVNDIIESELKNMGYSQYFSVIIDSQKSLDDLISSEVNVPVYVYNTRATGVKGDVRSYGKIASIPLINDYEKVREIVQKITKYDVTHVGMIINDRKDGKYSIGIRAVLTQDFMTADFAKIELSKLQTIAEKIMRINKEIKEVFYDVTSKPPATIEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S8</accession>
    <name>F4B9S8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S8"/>
    <dbReference type="STRING" id="933801.Ahos_1110"/>
    <dbReference type="KEGG" id="aho:Ahos_1110"/>
    <dbReference type="eggNOG" id="arCOG05996">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727316_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="157" mass="17680" checksum="B7F03C5C6299A9D6" modified="2011-06-28" version="1">MLCLVTIHMKPIDMKSLINYVALKILGGSDYLLNALEEYLVNGEGPAIVAHRYNISKHQLRGYAQRIIEKSGSECRAKKIIPILKQISVDVKPIITRDENGVYTCTICNTIVAREDAEEHVRKYHKDQLTLAIKSMMEKLDEIRAKKAKAVILTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U0</accession>
    <name>F4B9U0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartyl/glutamyl-tRNA amidotransferase subunit A, GatA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777071.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U0"/>
    <dbReference type="STRING" id="933801.Ahos_2285"/>
    <dbReference type="GeneID" id="10601792"/>
    <dbReference type="KEGG" id="aho:Ahos_2285"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="OrthoDB" id="7931at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF67">
        <property type="entry name" value="AMIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="287"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="393" mass="42918" checksum="24D4468F72130730" modified="2011-06-28" version="1">MRLEELNSKYDAFITIDEIKGRDKGKLKGLTFGIKDIILTKGIKTTAGSKILKDYIPNRNAWIVDKILEEGGTIVGKTNTHEFAIGATNTSSIAGPARNPYDPERISGGSSGGSAVAVALKMVDVGVGTDTGGSIRIPASLCGVIGFKPTTGIIPIDGVIPFSWTLDTIGFITRDIPTLRRVLDAVIPIENKHVLVSKVRTRPRLGVFLFKDDPASNSLKSILNKLSSYFDLIDLRLNFLEGFGSNVRGTIALAEGSSYHRDWIESTPGMYFPDVKELLMQGLQIRAIDYIDALRARRVIFEEYVRAFDSVDAIISPTTKIPAPKISEVVGREKEYRKLLVSNTELFNLVNAPSISLPVSKVNDLPIGLMVSGLPYEDGIILDIAEKILELAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9U7</accession>
    <name>F4B9U7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rad50</name>
        <name evidence="12" type="ordered locus">Ahos_2292</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777078.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9U7"/>
    <dbReference type="STRING" id="933801.Ahos_2292"/>
    <dbReference type="GeneID" id="10601800"/>
    <dbReference type="KEGG" id="aho:Ahos_2292"/>
    <dbReference type="eggNOG" id="arCOG00368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_2"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054990">
        <property type="entry name" value="Rad50_ATPase_DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041034">
        <property type="entry name" value="Rad50_Sulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="11">
        <location>
            <begin position="379"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="168"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="441"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE95163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="863" mass="100530" checksum="B99A90FF3957C83E" modified="2011-06-28" version="1">MKIEKIFLQNFLSHESSEINFKGSINAIVGQNGAGKTSIIDGIVFSLFSESSRGNIKNLVKKGKSTGIVQTEIRDGNNLYLIKRDIVNSSNDFIAKNNIGIARGRKEVDRKIQEILKLDKDILLSTVIIRQGEIENIFKELPDVLKKILKIENLEKLTSSTGPLYSVQKEIESELKYLENRKIEYENKKIEKEKLEKEIEESKKKLEDLEIKKIEKEKEIKDNSAKFDELKRKRDRYLELTGRLYSLIERKKSVEKDVDNYDKIKSEKEKLEKEIEENSYLEGGESLLSELHELRKSEKSLKDQLNTLEKEIEEYEQNLAKKNSLEDDAKRYEELKERKEKIEEAYNEYNSLKSLLNDKLKRKEKIENEIKSLGSIPSLDNVNKEIDNIEKEIDNLQNSKGAIKGRKEQLIQIIKNLENIKENRCPVCGRELTEEHRKKIKIEAETEVKDIEKKLSDIEKKVEELRARKGELSNLRLEIISKLTKLRKLNEDLTRISQEVQDIQTKLKELEKSYNEYSEIREKLTLLEPKYKEYLKVSNYDERALEEKKNRLGLIKAELDDLQKRIKGLLEKIGDEREFEGKLKLYKENKNKLEKLKEEFIKIESEKNELEKISKEVTELEEEIRQLNFNEEEFENLNKKLEDLNNERIAIEKEISTIEGKINASRDLLINLNSELNKIMEDLNKIPKLQNAINKVEKLRKILSGSGLQNYIISIFKSRIENNLNDILNMFNLSFSRVTINFEIGGKTQKGKVVIKAYNTAGNDLDIESLSGGERISIALALRIAIAKSLMDEIGFMIMDEPTIHLDEERKKELLNVIKYSMNIIPQIIIVTHDDEIKEISDYIISVTKKGDKSIVKQGEYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA13</accession>
    <name>F4BA13_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775926.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA13"/>
    <dbReference type="STRING" id="933801.Ahos_1126"/>
    <dbReference type="GeneID" id="71811289"/>
    <dbReference type="KEGG" id="aho:Ahos_1126"/>
    <dbReference type="eggNOG" id="arCOG03882">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1269949_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="222" mass="25765" checksum="BC6D22052906184E" modified="2011-06-28" version="1">MSVKRQVLSKNEISLRMSKLYELLTIAEDAHDILGYPPTTDFNFVYVKKKTEELSEYDLIKEGNAPYDYRQLYEKIKELYMEFLIKVMSNYADETMRTQIEYINFVLKSGEYVIFEGDIDKVTMPMPSGIASVHTHPGICIFSAPDIETADSLFVKGYVIIAVMNNECISYFLRKGPYTPEDQQELRKLQKKVKKAKTFEELKDGYTSFNSENVVFRTPLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA47</accession>
    <name>F4BA47_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA47"/>
    <dbReference type="STRING" id="933801.Ahos_1161"/>
    <dbReference type="GeneID" id="10600635"/>
    <dbReference type="KEGG" id="aho:Ahos_1161"/>
    <dbReference type="eggNOG" id="arCOG05992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_854237_0_0_2"/>
    <dbReference type="OrthoDB" id="34219at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="317" mass="35991" checksum="68865BC71FA7184A" modified="2011-06-28" version="1">MPIGKIATFDEGSINGSIEYSTLLKSTKVSSENRELLYTFYAEKKPVKAYIVIITETRDLTTKWRLWLNNFSLTKEFKPIDTVRVGGSYYNLNIYDVTHLTREGKNEFVVSHSSIEGIWIHIINSVLFYEVPEFKTNYKLRAGIYLIQPSEEIELDGEGKNYVVVRNPNKSILRVVNSTGTVTTIGGSLDSDEIEVKGHLSIIHESSQRSPAFLFLHYSENSITPSISINVNGKIGKDNKAVITLYNDGEVDLDRVLLNVMLNGITIHFKSFNNMKIGQKINYEFFVPKKGNVHLRIVGVKSGFRKILDKDLEWETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA83</accession>
    <name>F4BA83_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH-ubiquinone/plastoquinone oxidoreductase chain 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1197</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775996.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA83"/>
    <dbReference type="STRING" id="933801.Ahos_1197"/>
    <dbReference type="GeneID" id="10600673"/>
    <dbReference type="KEGG" id="aho:Ahos_1197"/>
    <dbReference type="eggNOG" id="arCOG01557">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147805_0_0_2"/>
    <dbReference type="OrthoDB" id="51573at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="entry name" value="NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004726">
        <property type="entry name" value="PRK06073.1-1"/>
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    </dbReference>
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        <property type="entry name" value="PRK06073.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="129" mass="14648" checksum="2585EDE6B894DA74" modified="2011-06-28" version="1">MSLVQSLVAFGLPSILTLALGYGGYKIISLMVPHNPTPLKISRFEAGNVPYGEGRLWFPLQYYGYLLMYVTIEPILILLFAIASAPLLGNFILFRNLMIIIGSFIVLIYPVMYYSITQINIIQNWELRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L5</accession>
    <name>F4B3L5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L5"/>
    <dbReference type="STRING" id="933801.Ahos_0009"/>
    <dbReference type="GeneID" id="10599449"/>
    <dbReference type="KEGG" id="aho:Ahos_0009"/>
    <dbReference type="eggNOG" id="arCOG02021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1830677_0_0_2"/>
    <dbReference type="OrthoDB" id="135517at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
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        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050229">
        <property type="entry name" value="GlpE_sulfurtransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43031:SF1">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="28"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="133" mass="15403" checksum="1D56A75D2FA7BD9C" modified="2011-06-28" version="1">MQVVEQYTTPYYKHILSLPPSSVRKLWKQNKITLIDVRTPEEYEDHHIPGAILIPLDYLEVLSKFLPDNDVAVICEHGNRALYATYGMPHIYKKKAIHMQYGMAGWMAMGYEVESGMNENGKKWMKLLDSIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N8</accession>
    <name>F4B3N8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dinitrogenase iron-molybdenum cofactor biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N8"/>
    <dbReference type="STRING" id="933801.Ahos_0032"/>
    <dbReference type="KEGG" id="aho:Ahos_0032"/>
    <dbReference type="eggNOG" id="arCOG02738">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167327_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="entry name" value="Dinitrogenase iron-molybdenum cofactor biosynthesis domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="Nitrogenase accessory factor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13646" checksum="FF90D90DC44C1471" modified="2011-06-28" version="1">MSMKIAVTYDKEQNLKPLDEAEIIGVIDEEKKVVEQYENPAYNVSKEATMGIILDLGVDAIIVKNKFLCPGSYMMSYGRLKYIPTEYNTLQEVLNNLEEVKKKIAEELPEEMYAEAYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X3</accession>
    <name>F4B3X3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Prangishvili D."/>
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    <dbReference type="HOGENOM" id="CLU_1987481_0_0_2"/>
    <dbReference type="OrthoDB" id="373204at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="13562" checksum="315A0596F4CB0359" modified="2011-06-28" version="1">MKGMWSSFTTLIILVATVFASLVVVFYAYSMLGAQNQSVIVTQASPGIIKDGQLYVSLQTNSQIKIVKLQVEGYCEAENVCLKPGLNNVSLTLPSGFSPSKCEVYSFTLVLCNGKSVIVNGKYYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M8</accession>
    <name>F4B4M8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M8"/>
    <dbReference type="KEGG" id="aho:Ahos_1375"/>
    <dbReference type="eggNOG" id="arCOG00569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143950_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="13607" checksum="FC354E40D2B3664B" modified="2011-06-28" version="1">MSPPDFLRHIANKVLTPNTLDPKRLDEVRKLLGEAENKYNFSAYGGNPKKLADYLLSPDFTELVFIIGIDLTKKLLEEIINDYDIEEVKNTAKKLLDEIDGYKEIENSDAILYNKNRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W1</accession>
    <name>F4B4W1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DSMO reductase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775043.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W1"/>
    <dbReference type="STRING" id="933801.Ahos_0234"/>
    <dbReference type="GeneID" id="10599674"/>
    <dbReference type="KEGG" id="aho:Ahos_0234"/>
    <dbReference type="eggNOG" id="arCOG01500">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_1_1_2"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10551">
        <property type="entry name" value="PsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF3">
        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="269" mass="30111" checksum="F783019C5A9E8F28" modified="2011-06-28" version="1">MSTPKPLHKLVIITDLNKCFGCNACQLNCKSWHTSSIFGPLTDLDPWGAEPFSLYLMRVIRIEQGTYPNVRTIPIPISCFHCEDAPCVKVCPTGAIYKRRVDGVVVIDYNVCQGFKYCITACPYGNIDFDPIEGVSKKCILCVDRLYDENLPEYERIPACVRGCPAGARIFGDLSDPNSEVSRIVAEYGGFVLGEQYGTKPEEEYLPIRGPSKGPFRNEPELPLEQRFAERTGAIDQAPDKVLQDAQQLGLTPEDLRTYKRNNKNVLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X0</accession>
    <name>F4B4X0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tatC</name>
        <name evidence="6" type="ordered locus">Ahos_0243</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775052.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4X0"/>
    <dbReference type="STRING" id="933801.Ahos_0243"/>
    <dbReference type="GeneID" id="10599683"/>
    <dbReference type="KEGG" id="aho:Ahos_0243"/>
    <dbReference type="eggNOG" id="arCOG01919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_3_2_2"/>
    <dbReference type="OrthoDB" id="15305at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="277" mass="31549" checksum="CB546C31549B0418" modified="2011-06-28" version="1">MTERTELRKDEERPLLAHIAELAMRLRRGLISLFLAFIIFFAFGYTTINIDGYVIPILYPSLFHSIADNLMVFFIHHELPKGMELLNLNPFDPLYASAYVSFYLALFISLPIIFRELWGFVSPGLYEHEKRLVKYTVLPATLLFLAGSLFAYFIIVPLMMRFVEIYTTALGVEPTLSLRAFVSTVVSLMMVTGIAFEYPLAMSILTYIGMVKADSWKKNWRWGVLGAFIIAWIISPGTTGGVIETTIGTILSVLYFVGVTSAKIIEKKRMKKEMQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X3</accession>
    <name>F4B4X3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0246</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
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    <sequence length="103" mass="11329" checksum="996891A24622B261" modified="2011-06-28" version="1">MKIVLSVDSEEKIPMSITAASHLSEMPETEEVEVVYLNGGIAAVTQRNRIAPLLKNEKVNVVACGTSMEARTKEELAPGVIYVPASLKEIIKRIQEGYIYLVL</sequence>
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            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="22"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="465" mass="49780" checksum="9847C1DD02D0F851" modified="2011-06-28" version="1">MKLTDAFKPLDDKKFDMFHIKSLLTTGMGVFTDGYLLSSISLVILDVMSTFGITKSNPSYTFWLGVLDGTVFIGAALGAIIFGILANKGRKTFYGVDVALMTIGALLQAFVTSPTELAIVRFLVGIGTGADYVLSPLIMAEHSNAKDRGKLIALGFGLMWSLGAIIASLLYLGISPFVTPSTLWRIILAAGAIPAASVIYLRRKVPETTRYLLRIKGDVKHFETVVKQIAGKTVNVNEDLKDKNPLLMYFKTSGRIFAVAAILWFLYDLTGYANGLFGPTLIARSIGIYNPAIFSLIVSAVFGFPGKFTGISLIDKVGRKPLQALGSLGEGIFLVAFALLLPKEAFLPAGLLLVIYGLHDFTGSVGPGIISTAGMLGVELAPTKVRSIVQAITVASGRTGAAIASFLFPVLFISVSKEVAMMFFAILMLIAAALTWFGVPETKGKPLELASQEEGIKENITIKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B525</accession>
    <name>F4B525_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Archaeal ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776242.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B525"/>
    <dbReference type="STRING" id="933801.Ahos_1444"/>
    <dbReference type="GeneID" id="10600940"/>
    <dbReference type="KEGG" id="aho:Ahos_1444"/>
    <dbReference type="eggNOG" id="arCOG03169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061108_0_0_2"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048907">
        <property type="entry name" value="WHD_MCM_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21100">
        <property type="entry name" value="WHD_MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="MCM C-terminal" evidence="2">
        <location>
            <begin position="285"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="351" mass="40525" checksum="5E13CE80375871B8" modified="2011-06-28" version="1">MLFDERPKDRKEDLFDREREVEEIINNIKRPLLLISGVRRIGKTSVLLVSLNEAKENYILIDCRKLKENYGRKELYSLFSEALSSKISYLKDVLEKVKGISIAGNYVEIKWGGKNYISLSSLFDELNKKRLIIAIDEAQRLRGPLSKEIKDAIAHAYDYDRNLTFILTGSEVGLLHELIDVENENSPVYGRYYLEITLDRFNKERSKEFLQRGFQEISIKVEDKIIEEIVNYFDGIPGWLTFAGNEYATKGKIEEVKKTAVKVAKGELENLIEEKTKKVSYITGKRYRNALKCIAKGENSWSKLLSCMEKEEGSTISTSVLDNIIQNLEKMSIIKDYEFLDPVYKEASKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B7</accession>
    <name>F4B5B7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mandelate racemase/muconate lactonizing family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B7"/>
    <dbReference type="STRING" id="933801.Ahos_0320"/>
    <dbReference type="KEGG" id="aho:Ahos_0320"/>
    <dbReference type="eggNOG" id="arCOG01168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03316">
        <property type="entry name" value="MR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046945">
        <property type="entry name" value="RHMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794">
        <property type="entry name" value="ENOLASE SUPERFAMILY, MANDELATE RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13794:SF58">
        <property type="entry name" value="MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="4">
        <location>
            <begin position="115"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="349" mass="39645" checksum="7C3604884BE92406" modified="2011-06-28" version="1">MYVKLSDKESYGWGESLIAGSGIIGAYSSIIRELIKPLIEEKYNIRSPFEFEEVFEKIMFSAGNCGIVTGAISAVEMAMWGLKARKMKIPLYELFGGKIKDYVKVYGSFPRFSKIDDLIKAVEFTVNKGFDFIKLHQPPSTTLEAVKAVRERFKEIKIAVDLNSPFDLSEAKNFADKIARYEVEWIEEPLWPPNDYFALEKLTKVSPVPIAAGENEYTLYGFRKLLESGVSYIQPDVAKIGGISKFLKVLDLASSYGVKVAPHDRPDSSIVSLIYTLNLALTRNEIKIVEYPISDLPKDLFNEPTFEKGYVRPPENIEINNSILNKYSYINRIRVLHFSDLNDKLIKYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D8</accession>
    <name>F4B5D8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide/dipeptide ABC transporter, ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0341</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775148.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D8"/>
    <dbReference type="STRING" id="933801.Ahos_0341"/>
    <dbReference type="GeneID" id="10599782"/>
    <dbReference type="KEGG" id="aho:Ahos_0341"/>
    <dbReference type="eggNOG" id="arCOG00181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_2"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000016">
        <property type="entry name" value="Oligopeptide ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067:SF3">
        <property type="entry name" value="MALTOSE ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43067">
        <property type="entry name" value="OLIGOPEPTIDE/DIPEPTIDE ABC TRANSPORTER, ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="318" mass="35769" checksum="BDC6D29DBD69FD95" modified="2011-06-28" version="1">MILRVENLSVSYYMEYGKIEAVKNVTFDLEKGEILSIIGESGSGKSTLAKAILRAIRYPGKIEEGSKIFFNGTDILSMKESDFKREILWKRISYVPQSSQNSLNNTMRVIDHFYDTAVSHGVNDKEEVKRTAKELLKMVRLDPDRVGKAYPFQLSGGMKQRVIIALSLLLNPELIIMDEAVSSLDVATQRYILEIIKDINKKTGVSIIFITHDISIAAFISSRIIVMYAGEIMEEGKTEEVIKNPLHPYTAGLINSVPSINSNVSQIKPIKEGYASIEGCPFNNRCEKAMEICKLSEPPIYNVNNRKVRCFLYGDNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D9</accession>
    <name>F4B5D9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type dipeptide/oligopeptide/nickel transport system, permease, DppB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0342</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775149.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D9"/>
    <dbReference type="STRING" id="933801.Ahos_0342"/>
    <dbReference type="GeneID" id="10599783"/>
    <dbReference type="KEGG" id="aho:Ahos_0342"/>
    <dbReference type="eggNOG" id="arCOG00751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_2"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43376">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43376:SF1">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="107"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="331" mass="36832" checksum="74466F477CB3B763" modified="2011-06-28" version="1">MNKVWLIRRIAMSFITILVTIIISWILIEYSPESPANAILSNIRANLAANPKFVKIYESEVQYYESLIPHGNPVIEALTYIANVLRGNLGLDVITEIPVTKLIAQALPWTIFVVSTSLIISFFLGIRIGQKMGYKRGTKIDSALMSTFTVMKSIPIYISGALLLYILGYNLHWFPTGGAYCPCVPMGLTFIGCVLYHSALPILTLTLANLATWALHMRANTIYTLGEDYVTFAEIRGVKDKTIETKYVGRNALLPLYTSLIIGIGFSFGGSVFIEEIFSYPGVGHLLYNAITNNDYTLEMGIFIIIITAVVFGVLIADLTYSLIDPRVRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E5</accession>
    <name>F4B5E5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CcmE superfamily domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775155.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5E5"/>
    <dbReference type="GeneID" id="10599789"/>
    <dbReference type="KEGG" id="aho:Ahos_0348"/>
    <dbReference type="HOGENOM" id="CLU_1559378_0_0_2"/>
    <dbReference type="OrthoDB" id="386335at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="18686" checksum="0DA978FDFBAB9B41" modified="2011-06-28" version="1">MRTLLKIGLIFIILSFVVGGVGAFFTLRTISSFFVIPTKVTVITIPSDSNFTIHYVNNGSAICLYYTNTSCVKILGIPSSATKIENNINGKTWSRSFIFLPTVKEGNITIVNPNPFPVKVTYKLSYIPPSYANPFRPFLDLLLSSIITLIVAGTLLIVGLILIILGVVLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G4</accession>
    <name>F4B5G4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, PadR-like family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776303.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G4"/>
    <dbReference type="GeneID" id="10601005"/>
    <dbReference type="KEGG" id="aho:Ahos_1505"/>
    <dbReference type="eggNOG" id="arCOG00002">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_3_2"/>
    <dbReference type="OrthoDB" id="56053at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR YQJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43252:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, PADR-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator PadR N-terminal" evidence="1">
        <location>
            <begin position="26"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="144" mass="16665" checksum="732820D21421A022" modified="2011-06-28" version="1">MQNNIKFELRTYHVHFHRGRGLRHIILYLLYTNGPLTGAEIMEKVEESSMGMWRPSPGSVYPMIRELEAEGLIEVVKTEGTKKYYGLTNKGKELLGGIPKERRIDNAITELESLVDYILDNWDSLNSEEKDRIRKIVEKLKSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I4</accession>
    <name>F4B5I4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776323.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I4"/>
    <dbReference type="STRING" id="933801.Ahos_1525"/>
    <dbReference type="GeneID" id="10601025"/>
    <dbReference type="KEGG" id="aho:Ahos_1525"/>
    <dbReference type="eggNOG" id="arCOG04502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136715_0_1_2"/>
    <dbReference type="OrthoDB" id="42032at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039018">
        <property type="entry name" value="VapC20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42188:SF1">
        <property type="entry name" value="23S RRNA-SPECIFIC ENDONUCLEASE VAPC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="147" mass="17000" checksum="A35DE849182A665A" modified="2011-06-28" version="1">MIFLDTSFLIAYLNKLDDNHEKACKIIKGIKSYGTVVISDYILDELATFLSYHVSKEFAIKVITRLVQLYMRKKLDIKIVDKKVFALAYLYFEKLKDREKLSFTDASTIAILDYFKIPYLASFDGDFDGLSTFIINKQSIINVTKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q7</accession>
    <name>F4B5Q7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">OrfB transposable element, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Q7"/>
    <dbReference type="KEGG" id="aho:Ahos_0383"/>
    <dbReference type="eggNOG" id="arCOG08540">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180511_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="66" mass="7409" checksum="36F9085A59A0A55A" modified="2011-06-28" version="1">MSVYNKFLLINNDLSRRCGQKGGSRDVSPVPLGSKESHDPPTVSGWLRAKSLHSIMNEHKMIEMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R4</accession>
    <name>F4B5R4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775195.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R4"/>
    <dbReference type="GeneID" id="10599830"/>
    <dbReference type="KEGG" id="aho:Ahos_0390"/>
    <dbReference type="eggNOG" id="arCOG07211">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1387510_0_0_2"/>
    <dbReference type="OrthoDB" id="379324at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21809" checksum="6B893D75B3CC3BF0" modified="2011-06-28" version="1">MNKWKTISIILIFIVVVESIIIFNQYRHVNLINNNSAVTEPEYRLNPVIGNYSFIINSTTQFVKVCNYTLIVAVVNINLTKVKVGDSFLLYPPINIGSTVCEALYNNPILNITIICNTLSEENGSQYLTFKIAINSSIIKAHGGATFLLCSHKIVATSLTTIDKNTFLFTVFKPDCSSEITLEFYIAPLSIGSKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R8</accession>
    <name>F4B5R8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R8"/>
    <dbReference type="STRING" id="933801.Ahos_0395"/>
    <dbReference type="KEGG" id="aho:Ahos_0395"/>
    <dbReference type="eggNOG" id="arCOG00713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850709_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09854">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052106">
        <property type="entry name" value="PINc/VapC_TA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38826:SF5">
        <property type="entry name" value="RIBONUCLEASE VAPC13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="131" mass="15388" checksum="8ADD35425D4B76E7" modified="2011-06-28" version="1">MKIMIDSNVFIYVLFSDPTYGERAKELLKTAEEEDAYSSTLVISQVLAHLERRKKVEIIPIFMDYLKQSGIKIIETTWEDFIGATRILQSSKLSYKLWDDAIIFNQMNRFGIDVVYSNDKDFDLFPVKREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5T2</accession>
    <name>F4B5T2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OrfB transposable element protein, IS605-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <lineage>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5T2"/>
    <dbReference type="KEGG" id="aho:Ahos_0409"/>
    <dbReference type="eggNOG" id="arCOG00679">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AEE93297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="100" mass="11462" checksum="386231B320F4CDFE" modified="2011-06-28" version="1">MYLNTSFSKFLLMTRRVKAIRATVSMKIAVSDSLLALVNNYVKAIRFTLFWLKENVPNLEEKGALGKVHEELCTRLRGEYNLPSKVAEDCYRDAISIYKG</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        </source>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="10222" checksum="4C9386DAAE8E54DF" modified="2011-06-28" version="1">MKDFRSKKVAIVANCILNQNSKVIGFAKYKGMVKDIVDLLYEYNYGILQLPCPETLFAGARRWWQVRDQYDTEGYREHCRMLGFGLH</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="GeneID" id="10599867"/>
    <dbReference type="KEGG" id="aho:Ahos_0427"/>
    <dbReference type="eggNOG" id="arCOG07307">
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    </dbReference>
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    <dbReference type="OrthoDB" id="42557at2157"/>
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            <dbReference type="EMBL" id="AEE93315.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="11606" checksum="0BC92E39DCEF522B" modified="2011-06-28" version="1">MLVLSPHLRKHVEDINNYMRKFNTVIDPLGQEVLSFLEGVKGIEQVPNVRLGEKERWRVIIHFKPCAKIRYIIARKDNELILVTAHPDPDAENFIEFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5W3</accession>
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        <submittedName>
            <fullName evidence="1">Conserved conjugative plasmid protein</fullName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AEE94461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5W3"/>
    <dbReference type="KEGG" id="aho:Ahos_1578"/>
    <dbReference type="HOGENOM" id="CLU_2115344_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="114" mass="12907" checksum="63B58D593D5DBD42" modified="2011-06-28" version="1">MENIGLPYTSFPNFSLEYLTGFSFNNSSLSTINMRYSKLENSLATNLAKSLASLTFFIVNFNSHFSQITTKDIENVFKNISLFVYYLTRNNSCNNDNIGSSNHSCDYVLLQTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5X8</accession>
    <name>F4B5X8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide biosynthesis protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1595</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5X8"/>
    <dbReference type="STRING" id="933801.Ahos_1595"/>
    <dbReference type="KEGG" id="aho:Ahos_1595"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046380_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052556">
        <property type="entry name" value="PolySynth_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43424:SF1">
        <property type="entry name" value="LOCUS PUTATIVE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
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        </location>
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        <location>
            <begin position="200"/>
            <end position="222"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94476.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="325" mass="35744" checksum="8894FEEB8A39D709" modified="2011-06-28" version="1">MGISIIAVLSHNIYFFVEIWTLGGIISVSMNCAFLSRKVGIILASFNFAFLFKHFKEGLPLYLSSSANFLSSQGDRVITAYLLGSYYLGLYQFSALIAGVPLMVLGALGGVLLPTASFYKALGKDEKKMSSISFRVLSLITFLAVIISIPTAVLVVEKFFPAYTSGIKVLILLLLATALPFPIISLTNFIIAARKSLRPFLMLSLLNGGLVLLTSFLLIPRIGIMGGAISQVIVSTISSSFVLVYALRTSTFILGRRETTLLTVMPLIGIYEVFIDPLYLDLLLLLSILFLFKFFKIITVDYMKLIQSFLPSKLKFVALILKIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B628</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name evidence="1" type="ordered locus">Ahos_1648</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B628"/>
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    <dbReference type="GeneID" id="10601148"/>
    <dbReference type="KEGG" id="aho:Ahos_1648"/>
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    <dbReference type="HOGENOM" id="CLU_2821080_0_0_2"/>
    <dbReference type="OrthoDB" id="39177at2157"/>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="67" mass="7851" checksum="BFAD156AF7AFE677" modified="2011-06-28" version="1">MIDPKLIIKLLEDRQRPRGSKIKDDDLKRLARLGEENIRLLEELGCWKIKGGVIYYKTGCLGNYFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="Gene3D" id="3.10.590.10">
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        <property type="match status" value="1"/>
    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AEE93325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="24559" checksum="7D5430B8B2A05EAF" modified="2011-06-28" version="1">MLYLIKVPRKKSSFTLSFLGLSMIIEEMQKKGFKKLTGPPEDWLKTFTSMEWGFREKERLKREWDKIQPGDIFIFHSMKPEYISADFLPTGIIGVGVVGEKRIGIDEEAYFEDKNLRPLRIVFKEIWWFGEYEKISTEKFPIKAKKGTDFLIRELYYLLKNCVTFQEMREHDCVISTQGAIQNVSIEKQLRLIELIKPRLREPIIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B648</accession>
    <name>F4B648_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">Ahos_0448</name>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B648"/>
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    <dbReference type="GeneID" id="10599888"/>
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    <dbReference type="eggNOG" id="arCOG04216">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052138_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="390" mass="46079" checksum="91EE91F2E1B2D8C7" modified="2011-06-28" version="1">MIEEKLKEVYGIDKYGKNVPEWAEKIKYEFVDEVMQDKLREAKIWINELYKVFEELQERLKVKGMLFSREMKSFLDDPRRHLVKKLFIYYHDLIRGKLSVEDFIIKGKQAIVSSFNSNMRSIYQSWGLASIVLLLAEKGNYELIYPEHGYLDFDRSGKQKSGTIPPNIVVGNVVTSFSFFLEAPRPITWEDGGDLEKVWKFYSLVRPDMLIYEGTEINIVDPQGDIPIKRPDYIVEYKELPNWWKRWRYLKDYKPLDGNEWRARWLKGLYEGFAEILGVEAKDLPSFSEGKSKRIKEYKVIELYEAIYKPRKGGVVISRVPVEDEIKREIINDNVTLIDDVGFNQGRLEILIEKMTKGIKSTYDLKELAYKFAMSKKEEFIEWMRSQGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B653</accession>
    <name>F4B653_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable thymidylate kinase</fullName>
            <ecNumber evidence="7">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tmk</name>
        <name evidence="9" type="ordered locus">Ahos_0453</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B653"/>
    <dbReference type="STRING" id="933801.Ahos_0453"/>
    <dbReference type="KEGG" id="aho:Ahos_0453"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="8">
        <location>
            <begin position="11"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="215" mass="25039" checksum="9218F6BDD33D0E0D" modified="2011-06-28" version="1">MEWLKGILIAFEGIDGSGKSSQASLLKDWIEMKRDTYLTEWNSSEWIHDIIKEAKKKNLLTPITFSLIHATDFTDRYERFILPMLKTGFVVISDRYIYTAYARDSVRGVDIEWVKKLYSFAVKPDVTFYIRVSAEVALERIKKARRNIKPQEAGYDVFPNLPPEEGFLKYQTMILNAYDKIAKEENFVIIDGNKTPREIQMEIRKVLGEILDNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B663</accession>
    <name>F4B663_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIGR00266 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0463"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24230" checksum="107D7F03FD0F8C89" modified="2011-06-28" version="1">MQYQIIGDDIQYVKVFLNPGEKIYADAGHIMSKSSSVTIQAKMRGGILSAIKRELTGATFFVTELMGPGEVDIAGVFPGKIVPIQLNGKGILAESHSFLFAEDSINYDARLAPLAPAILGGEGLFLATFSGVGQVFLHAYGGLYEINLMPGQSIDIEASHLLALEEGMQFTVTRVGGFKTMLFGGEGLYFVRVVGPGKVWIHSITAQQMASALMPFLPGGQKGGLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B1</accession>
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        <recommendedName>
            <fullName evidence="3">DUF2250 domain-containing protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B1"/>
    <dbReference type="STRING" id="933801.Ahos_0511"/>
    <dbReference type="GeneID" id="10599952"/>
    <dbReference type="KEGG" id="aho:Ahos_0511"/>
    <dbReference type="eggNOG" id="arCOG03698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1324070_0_0_2"/>
    <dbReference type="OrthoDB" id="45652at2157"/>
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        <property type="entry name" value="DUF2250"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017139">
        <property type="entry name" value="UCP037213"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10007">
        <property type="entry name" value="DUF2250"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037213">
        <property type="entry name" value="UCP037213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="24667" checksum="2B5C6291EBFEC6C6" modified="2011-06-28" version="1">MEEELKDKLKEVLKDKRYLQVLQHLKKANVDYGKSIMLNTKIPIQEVVDILDKLEALGLIERVHGATLKNTEAKFKLSSEVHKHHTYYRLTREGDHLLRNLDEKELIKAYIDLVKNDDLAKDILRLAEELNADHALTYAKLTHKTLEEVTPKLEELERMGLLEEAKAKIIKFGDRKSKPKKETRTHHKYYGLSRIGELVVREMKRRGIIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G5</accession>
    <name>F4B6G5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6G5"/>
    <dbReference type="GeneID" id="10601208"/>
    <dbReference type="KEGG" id="aho:Ahos_1708"/>
    <dbReference type="eggNOG" id="arCOG00761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_1_2"/>
    <dbReference type="OrthoDB" id="40408at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
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        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
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        <property type="entry name" value="tRNA_TsaA"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="147" mass="16934" checksum="201C39E497BA05E1" modified="2011-06-28" version="1">MDCCFNYIGFVRREDNSASRNSVVRIVIKEEYVEGLKGVEEFSHLIIIYHLHLANLNKGLKRVRGGVEVGVFATRSQHRPNPIGISVVELIKREGNTLYVKGINAFDGTPVLDIKPYDEWDSILNPKVPEWHKIEMRSRITDSFYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6H6</accession>
    <name>F4B6H6_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776511.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6H6"/>
    <dbReference type="GeneID" id="10601219"/>
    <dbReference type="KEGG" id="aho:Ahos_1719"/>
    <dbReference type="eggNOG" id="arCOG01841">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1615218_0_0_2"/>
    <dbReference type="OrthoDB" id="376096at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="164" mass="18699" checksum="E5D1A605ED8BA4C5" modified="2011-06-28" version="1">MDELERKIINALEKQDMNSLSGLVKSLSMMANFDGIVIYYVTFDNKDGKIVVPKESEDYQRLMNAVAPTSMTSSIEKMLRAMMGIEEEPEKETQYEVQEEEDFEIIPKEDELFVVGRVEEEPEVRIESGVLDVGKKAITLPPGNWKIVEKKFKNGILTVHLQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J5</accession>
    <name>F4B6J5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PEBP family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AEE93405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="YbhB/YbcL family Raf kinase inhibitor-like protein"/>
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    </dbReference>
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        <property type="entry name" value="UNCHARACTERIZED PROTEIN YBCL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="148" mass="16841" checksum="BF3988B826FE7044" modified="2011-06-28" version="1">MQVKSSFKNEEFIPVKYTCDGEDISPPLEWDKVDNAKTYAIIVEDPDAPAGTFIHWVIYNIKGNSLPENVPKKEKSDYGIQGINDFELVGYNGPCPPRTHGAHRYYFNVYALDTELDYKSKITADELKEMMEGHILATGSIMGKYKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K0</accession>
    <name>F4B6K0_ACIHW</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0522</name>
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    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K0"/>
    <dbReference type="STRING" id="933801.Ahos_0522"/>
    <dbReference type="KEGG" id="aho:Ahos_0522"/>
    <dbReference type="eggNOG" id="arCOG00084">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582937_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="32"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="162" mass="18431" checksum="1A84853862F8414E" modified="2011-06-28" version="1">MEPKLIITTLPGKDKKCKAEILDRLLIKDENVQIDEIKQNVFLIYSKLSPLEAYGLIISAPPSCIGRIFTIDKISDLSNIFEDAEKLLSSKKFKKFYVECINRGNKQADCRSIEIGIGLRMKGKLQVDYKNPDYILFVNLIGNKVYLSIMRKGQEKVSVNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M2</accession>
    <name>F4B6M2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH-quinone oxidoreductase, B subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0544</name>
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    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775348.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M2"/>
    <dbReference type="STRING" id="933801.Ahos_0544"/>
    <dbReference type="GeneID" id="10599987"/>
    <dbReference type="KEGG" id="aho:Ahos_0544"/>
    <dbReference type="eggNOG" id="arCOG01554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NADH-quinone oxidoreductase subunit B"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
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        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
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        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="6">
        <location>
            <begin position="49"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="170" mass="18982" checksum="ED520F3A8C4119DE" modified="2011-06-28" version="1">MTDQILLTGNLKEAARKAAQWLANRKPIRDLRDWGTAFSLWPPHFTTSCCGAEFGAFAAARFDAERFGMLPFSSSRQSNILVIEGTMTRKMARAARIVYEQMPEPKYVMAIGACSLEGGIFWNSYNTVLPSEVGIPVDIYVPGCPIRPEAIARGLLMLQKKIRHQSTIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P4</accession>
    <name>F4B6P4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0566</name>
    </gene>
    <organism evidence="1 2">
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6P4"/>
    <dbReference type="STRING" id="933801.Ahos_0566"/>
    <dbReference type="KEGG" id="aho:Ahos_0566"/>
    <dbReference type="eggNOG" id="arCOG05985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253637_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="24489" checksum="97898B7CC4B8A55F" modified="2011-06-28" version="1">MCNLSLIGLKLTLAYSKEEELLPILPILDKEIDKGIEIEAIKIKEEEIKFKYTNFDLYYLPLPDLNYIETKILSNGAYAVNSLGLDSLKNEVCINSNSTEFYLLKVLMNYRGIPNGRDNCNGSKFITKDFKISLSDKWKEVCGDLPIVLNVIGSNLPEDVISKVKIIIRESASLQEAKGVITPISKELGLKGRKAIDCFFDLCRKKNLCNVNNYVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6P8</accession>
    <name>F4B6P8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6P8"/>
    <dbReference type="STRING" id="933801.Ahos_0570"/>
    <dbReference type="KEGG" id="aho:Ahos_0570"/>
    <dbReference type="HOGENOM" id="CLU_1159001_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="239" mass="28026" checksum="EDA89DD8E8DF0393" modified="2011-06-28" version="1">MKKLSQKFKFIKLFNIKGFGGSNEEKITDLENLSIKILIFNYLTFISSLIYLRFYIQTLAIALLVSTLSLSYLTIKNKINFTQKVNLLSLTSIIPLLLPITIEARISASLAIFSVLNWNFNKEDVYHQFLKRKEEKIKEMKRELLQGSKIYKATIKRLNKLERKLYEFYQKLQVFYFILYFLPLLTLPIIKSSGIIAYEEGVLNILIMSSMLSLLLRNSLFDYKNILYISLISALIWFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T6</accession>
    <name>F4B6T6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CRISPR-associated DNA-binding protein, Csa3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T6"/>
    <dbReference type="KEGG" id="aho:Ahos_1752"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107894_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010163">
        <property type="entry name" value="Csa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054588">
        <property type="entry name" value="Csa3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22662">
        <property type="entry name" value="Csa3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Csa3 N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="223" mass="25465" checksum="750EA1E6BD057FE6" modified="2011-06-28" version="1">MKSYFVTLGFNESYLLRLLNETSAQKEDKLLIVVPSPIVEGTKAAIENLRIQTLRLRYPNPEVIEIKISTFDDILSQLLDNLLPLQEPIISDLTMGMRLMGALILIAILVSRKKFTIYLRDENGGSRVVSFNSSDINALFKEYSSEEFKMLYNRFKNNTISTFELSKILGKSEKTIQNKISELKKLGILTQKGKDRTVELTSLGLNVIKLIKNSRLYIENKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6V8</accession>
    <name>F4B6V8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved pro-fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776566.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6V8"/>
    <dbReference type="GeneID" id="10601275"/>
    <dbReference type="KEGG" id="aho:Ahos_1775"/>
    <dbReference type="eggNOG" id="arCOG07733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152732_0_0_2"/>
    <dbReference type="OrthoDB" id="44117at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="119" mass="13013" checksum="74F2AC106CBD2FCC" modified="2011-06-28" version="1">MEFLSLLIFVLGVTVAILTIYLSPDFAKAKPNKRNLSIIFLSIILVGIGLWLFHIDHYTYICSSAPQNVNCSVPSLGTVYAPSKVIPPPSYASLWPVFLASGIGLMGFSVLIQIVRSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6W4</accession>
    <name>F4B6W4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1781</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776572.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6W4"/>
    <dbReference type="STRING" id="933801.Ahos_1781"/>
    <dbReference type="GeneID" id="10601281"/>
    <dbReference type="KEGG" id="aho:Ahos_1781"/>
    <dbReference type="eggNOG" id="arCOG00196">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="97750at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="291" mass="32476" checksum="B5C88252FC5AC900" modified="2011-06-28" version="1">MSVIKANNLTKRFGDFEALHGLSFSIEEGSITAVVGPNGAGKSTLLKIISGLIKRTSGEIKVLGEDPWKSQKLPRMLSVILDKPYLPQFLTVEEVIKEATSEFDADYSYALSLVEELNLTNFLKSKIKDLSTGTKQKVQIVFSLIKNPKIIVADEPTANLDVASRFEVYNIFLKLREKMHTTILISSHIASELIAISTHILGINNGVLRYVGEISKMIRKDLLEEFYITVDNVQRAVSLLKSYTVEVYGNQLKVRGNLVNIVSELVNNGVRIFYIRNSILDKSVQGEVGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y4</accession>
    <name>F4B6Y4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mercuric reductase</fullName>
            <ecNumber evidence="4">1.16.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Hg(II) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">Ahos_1802</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Hg + NADP(+) + H(+) = Hg(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23856"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16170"/>
            <dbReference type="ChEBI" id="CHEBI:16793"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776592.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y4"/>
    <dbReference type="SMR" id="F4B6Y4"/>
    <dbReference type="STRING" id="933801.Ahos_1802"/>
    <dbReference type="GeneID" id="10601302"/>
    <dbReference type="KEGG" id="aho:Ahos_1802"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_1_1_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016152">
        <property type="term" value="F:mercury (II) reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050787">
        <property type="term" value="P:detoxification of mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021179">
        <property type="entry name" value="Mercury_reductase_MerA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02053">
        <property type="entry name" value="MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014">
        <property type="entry name" value="MERCURIC REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43014:SF4">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE RCLA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 17" id="KW-0274">FAD</keyword>
    <keyword evidence="7 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="11" id="KW-0476">Mercury</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14 17" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="19">
        <location>
            <begin position="2"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="18">
        <location>
            <begin position="317"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AEE94677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="435" mass="47702" checksum="841CD50FFF46930C" modified="2011-06-28" version="1">MKDLVIIGYGAAGFASLIEANELGIKPVLIGYGPIGGTCVNVGCVPSKRLLHLGEKGGDFFSSFEDTRQFVRKARKEKYEDVLSYYDVELIEGKAHFISPHEVKVGDKVIEGKKFIIATGSSPFIPEIPGLKDLGYWTNVEALNPDRKISSLVIIGGRAEALEFSQMYRNFGVEVAILQRSKTLIPDWEPEISLEIQRVLEEEGIYVITDVKVKEVKKGESGKLVITNMGDIEADEILMATGRKPNVDLNLSSAGVELKEKGGIKVNDELQTTNPNIYAAGDVIGDKMLEALAGYEGTIAVRNAIKGEHKKIDFLSVPQVIFTRPNLARVGLNSFDGDFDSRTVKMKDVVKAQIIDAEDGLIKMVVEKGSKRIMGVHVMAENAAEFIGEAALAIKHRMTIDDIIDTVHVFPTVAESLRIVALAFYKDVEKLSCCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z3</accession>
    <name>F4B6Z3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IS607 transposase TnpA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z3"/>
    <dbReference type="STRING" id="933801.Ahos_1811"/>
    <dbReference type="KEGG" id="aho:Ahos_1811"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_4_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050639">
        <property type="entry name" value="SSR_resolvase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461">
        <property type="entry name" value="DNA-INVERTASE FROM LAMBDOID PROPHAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30461:SF2">
        <property type="entry name" value="SERINE RECOMBINASE PINE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="3">
        <location>
            <begin position="4"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14851" checksum="D1F74FA6A3298DB6" modified="2011-06-28" version="1">MTRQIAIYTRVSPTQKDYLERQLDALREWVKKTFGEVSVVEIKDISSGLKEDREGLKKLLAKRRQIDAVVVAYKDRLTRFGFEVKLFKAYGANVVVAFQDDYVRELLEDFVEIVKSFAPTAKSMRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B713</accession>
    <name>F4B713_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mut7-C RNAse domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0608</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B713"/>
    <dbReference type="STRING" id="933801.Ahos_0608"/>
    <dbReference type="KEGG" id="aho:Ahos_0608"/>
    <dbReference type="eggNOG" id="arCOG04290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112469_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081">
        <property type="entry name" value="MUT7-C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39081:SF1">
        <property type="entry name" value="MUT7-C RNASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mut7-C RNAse" evidence="2">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="184" mass="21444" checksum="F43A0E7FE7D8F9E7" modified="2011-06-28" version="1">MCLDNMQSKFIADAMLGKLARWLRILGYDTYYSNDIEDWKVIKIAEKDKRVILTRDRGLCIRAKKKGLECFLIPPDYDIISILAKLSIKYGIDLEARIEASRCSECNGILEKVDENRWRCTRCKKEYWKGKHWKTIEEILIKAKSKKEKDELSYNSRTRNRGGQNTSQDSKTSNNEKIGVAKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B718</accession>
    <name>F4B718_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lig</name>
        <name evidence="17" type="ordered locus">Ahos_0613</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 14 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775417.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B718"/>
    <dbReference type="STRING" id="933801.Ahos_0613"/>
    <dbReference type="GeneID" id="10600060"/>
    <dbReference type="KEGG" id="aho:Ahos_0613"/>
    <dbReference type="eggNOG" id="arCOG01347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005138_6_0_2"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07901">
        <property type="entry name" value="Adenylation_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000007">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000012">
        <property type="entry name" value="Probable DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00407">
        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 14" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="16">
        <location>
            <begin position="339"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="14">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE93501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="600" mass="67661" checksum="349B811022F48B30" modified="2011-06-28" version="1">MEFKIIAEYFDRLEKISSRLQLTSLLADLFKKTDKNVIDKVVYLIQGKLWPDFLGMPELGIGEKFLIRALSIATSVSDDEIEKMYKSVGDLGQVAFDIKQKQQSASILAFLGAQKASKPLTVEKVYDDLTKVATSTGEGSRDIKIRLLAGLLKDASPLEAKYLVRFVDGRLRVGIGDATILDALAITFGGGQNFRPIVERAYNLRADLGNIAKILANGGIEQLKNIKPQPGIPIRPMLAERLSDPAEMLSKVGNLALVDYKYDGERGQIHKAGDKIFIFSRRLENITNQYPDVAEYISKYVKGNEFIVEGEIIPVDPETGEMRPFQELMHRKRKSDIHEAIKEYPVNVFLFDLMYYEGEDYTVKPLSERRKKLESIVEDNDYVHIATHIITDNVEKLKEFFYQAISEGAEGVMVKSLAPDAIYQAGSRGWLWIKFKRDYQSEMADTVDLVMVGAFHGKGRKGGKYSSFLMAAYNPDKDVFETVCKVASGFTDAELDDLQKKIAELKRDSPHPRVVSTMVPDVWLTPALVAEIIGAEITISPLHTCCKDQYAEGGLSIRFPRFIRWRPDKSPEDATTNREILEMYKSQLKKIEEKPSDQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B720</accession>
    <name>F4B720_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775419.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B720"/>
    <dbReference type="STRING" id="933801.Ahos_0615"/>
    <dbReference type="GeneID" id="10600062"/>
    <dbReference type="KEGG" id="aho:Ahos_0615"/>
    <dbReference type="eggNOG" id="arCOG00755">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_4_1_2"/>
    <dbReference type="OrthoDB" id="168391at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF287">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="367" mass="42306" checksum="4E47D09ADC46C785" modified="2011-06-28" version="1">MIIIGAGGHGLSLAYHLVKKGVKGIILVEQSRIGYGSSSRNASRFRYHFYSKENIEFALEGIKYLISQKKELKYNPLIYKTGYLWLLHDENLIESFKKLHSLWSSYSIGGKFLECREFEFLKVNRTCYFAPQDGAFHHDYILYSYYEEIKDKVDIKYAKVSKILHNNGKITGIKLESGEEIKDNEVIVTAGAWSGELLKTANINLPIYPDRKEIFITEDVKFEIKPLVIDFKREIYFSHTLKGEIIGGIEKEDIGFKEFSISFLDSIEYLKRLREVVKGIEGIGILRGWSGYYEMTPDHSHVMGYGNDWPEGLFIDAGYSGHGMMFAPYAGKIMADLIADNKKNRFIQIFSPDRFKEEKLIEEKMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A5</accession>
    <name>F4B7A5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1852</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A5"/>
    <dbReference type="KEGG" id="aho:Ahos_1852"/>
    <dbReference type="eggNOG" id="arCOG10435">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313825_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="11019" checksum="F4B1278FB042E777" modified="2011-06-28" version="1">MYRKTIQLKKVNEVEWTLCIRDNIMLRGKLKVIIYLLEKIEITFRNPDGYGVIELNQGKILNISYKGILESKIKEKLTQCSPILVDTIKEKTCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D5</accession>
    <name>F4B7D5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rieske (2Fe-2S) domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1882</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7D5"/>
    <dbReference type="STRING" id="933801.Ahos_1882"/>
    <dbReference type="KEGG" id="aho:Ahos_1882"/>
    <dbReference type="eggNOG" id="arCOG01720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_1_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014067">
        <property type="entry name" value="AioB/IdrB_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02694">
        <property type="entry name" value="arsenite_ox_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="98"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="208" mass="22160" checksum="B8C9A2C6BD3E64B6" modified="2011-06-28" version="1">MMSNEKKNEGKKPDPDRRAIIIGGAAAVAGVAAGIVIGSQAFPKLLTKTEVQPEVTKEVVPQYITKTVTQVEKYVKQLVANVSDFPTAGTTKMTTYMGYSVILIKTGVPSIGGVGPDNDIVGFSGYCAHMGYPLSYDPNTNCLLCTMHFSQYDVTKGGMQVIGHPNQYLAQLVLEYDSSTGNIYALGFNKLVYGAYNNVLQGSGGATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H3</accession>
    <name>F4B7H3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="5">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ubiX</name>
        <name evidence="7" type="ordered locus">Ahos_0691</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl phosphate + FMNH2 = prenylated FMNH2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052362">
        <property type="entry name" value="HTH-GbsR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH marR-type" evidence="4">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14470" checksum="57F41681A83AFCDA" modified="2011-06-28" version="1">MEFKIQFPDGRQVDFHKLVEFLYGITDSEMNILHLLLSTDEKLSVEDISERLKIAKTSISKPVNILLSKGLIMRDKTEGEGKRRPKYLYYTDKNAVYNKIVNDLEAIAKIFCEKYKSHVESVVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R6</accession>
    <name>F4B7R6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776728.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R6"/>
    <dbReference type="STRING" id="933801.Ahos_1940"/>
    <dbReference type="GeneID" id="10601439"/>
    <dbReference type="KEGG" id="aho:Ahos_1940"/>
    <dbReference type="eggNOG" id="arCOG01192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153285_0_0_2"/>
    <dbReference type="OrthoDB" id="40849at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="143" mass="16756" checksum="936511053FD11A2D" modified="2011-06-28" version="1">MNFDDIALSYIRQAEERIELAKTELNKKNYHIVVRFCQEAVELLLKASLKLVGVEVPKFHDVGPTLKTNSSKFPDWFGHKIDLFASYSRSLRKERELSMYGEEETGTPPELLYSEYDANEALRMAQEVLEYVKKLYEEKKKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U2</accession>
    <name>F4B7U2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DZANK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776754.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U2"/>
    <dbReference type="STRING" id="933801.Ahos_1966"/>
    <dbReference type="GeneID" id="10601465"/>
    <dbReference type="KEGG" id="aho:Ahos_1966"/>
    <dbReference type="eggNOG" id="arCOG07910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2313620_0_0_2"/>
    <dbReference type="OrthoDB" id="44188at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DZANK-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="99" mass="11183" checksum="BAF7DBEDE7A77BBC" modified="2011-06-28" version="1">MPQTCPMCGATLRPDLNYCEVCGADVSIYDQVLQLIMNAQPFQGPIFNQPVPQQPMMQQPVQQPMQQGIVCPNCGQLNPPDAKRCMYCGAELHKHHHFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7V4</accession>
    <name>F4B7V4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl30e</name>
        <name evidence="7" type="ordered locus">Ahos_0745</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775547.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7V4"/>
    <dbReference type="STRING" id="933801.Ahos_0745"/>
    <dbReference type="GeneID" id="10600202"/>
    <dbReference type="KEGG" id="aho:Ahos_0745"/>
    <dbReference type="eggNOG" id="arCOG01752">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130502_1_0_2"/>
    <dbReference type="OrthoDB" id="10759at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="8"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="105" mass="11466" checksum="4093762B929FD300" modified="2011-06-28" version="1">MSQQISFESELKTLLKTGKVIFGARRTIKNLKLGKIKAVIIASTLRTDLKADILYYSKMSGVPVYEYPGSGWELGTLAGKPFMVSTIGVENEGDSKILQLAKPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X5</accession>
    <name>F4B7X5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylosuccinate synthetase</fullName>
            <shortName evidence="7">AMPSase</shortName>
            <shortName evidence="7">AdSS</shortName>
            <ecNumber evidence="7">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purA</name>
        <name evidence="8" type="ordered locus">Ahos_0766</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775568.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X5"/>
    <dbReference type="STRING" id="933801.Ahos_0766"/>
    <dbReference type="GeneID" id="10600225"/>
    <dbReference type="KEGG" id="aho:Ahos_0766"/>
    <dbReference type="eggNOG" id="arCOG04387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_2"/>
    <dbReference type="OrthoDB" id="372247at2157"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003295">
        <property type="entry name" value="PRK04293.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="11"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="247"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="279"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="319"/>
            <end position="321"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="337" mass="37514" checksum="1FDCAABBC9AD0D44" modified="2011-06-28" version="1">MLNILVGGFYGDEGKGKIASYLSLKDSPHIAIRTGSINAGHTVCYNNNKWKIRIIPSAFVNKSTKLMLGPGALTSIAELIKELKETNSEDRFFMDYHVGIITEKEIYEERNDENLMKSVGSTGQGVGYAEAKRILRILKLAKDYPELSKYTIDVPNTLIEEIEKGSEILAEGTQGTYLSLYHGEYPYVTSRNTTSSGILSEIGIGPKYVKDIIIVFKSFVTRVGNGPLEGELNEEEAEKLGLIEIATVTGRKRRVAPFNIKLAKKAIQLNTATQVAITKLDALFKDAYKVREYEKLPSEAKKWIEDLEDQLKVPITLIGTGEDAMDTIDLRKEKLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y4</accession>
    <name>F4B7Y4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylyl cyclase CyaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y4"/>
    <dbReference type="STRING" id="933801.Ahos_0775"/>
    <dbReference type="KEGG" id="aho:Ahos_0775"/>
    <dbReference type="eggNOG" id="arCOG01723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105244_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028:SF2">
        <property type="entry name" value="CYTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="SI:CH211-156B7.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="6"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="21983" checksum="1639548494A043BE" modified="2011-06-28" version="1">MQMADIIEREVKLKLESPTLTELYDKLLNDGMQLIKKETEEDIYFNSEFRDFRKTDEALRIRKTNSGIEFTYKGPKIGKISKSREEITVNVNNADNLIKILEKLGFKPVYTVVKNRIFLKDGEFIICLDSVKDLGEFIEIEIPNSTEEKLIEYVNLFLKKYKIRATQIKKSYLELLVSKNEENNSNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z6</accession>
    <name>F4B7Z6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-ribbon containing, Elf1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z6"/>
    <dbReference type="STRING" id="933801.Ahos_0787"/>
    <dbReference type="GeneID" id="10600247"/>
    <dbReference type="KEGG" id="aho:Ahos_0787"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165872_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK14892.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
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    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="101" mass="11217" checksum="CC535AB7A4231DE0" modified="2011-06-28" version="1">MGGKRKKRTKIIKPKPKLPKTFECPRCGRIAISVEIKNGIAHIKCGNCGLSADIEVPPVFDEANAYGKFIDLYLDGKLEIKESNKEESTNEGEDKQVHTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B816</accession>
    <name>F4B816_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0807</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B816"/>
    <dbReference type="STRING" id="933801.Ahos_0807"/>
    <dbReference type="KEGG" id="aho:Ahos_0807"/>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
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        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="MJ0586"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00270">
        <property type="entry name" value="multiprotein bridging factor aMBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
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    </dbReference>
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        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="73"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="154" mass="17702" checksum="C4F93BDCA7CA741A" modified="2011-06-28" version="1">MCGMPIRGKGFTVVYEGSIITVCEVCYSKIKKSAKIYEEKPKKAQIIKQQSQNINNKKPEIEVEVVDDYYKVIKSARERLGLTTKQLADKMKVSENIIKRFEQGKLKPTIEQAKELEKILSVKLLYTVSNEETQRGEKSFELTLGDIVNIREKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B835</accession>
    <name>F4B835_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776774.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B835"/>
    <dbReference type="STRING" id="933801.Ahos_1987"/>
    <dbReference type="GeneID" id="10601486"/>
    <dbReference type="KEGG" id="aho:Ahos_1987"/>
    <dbReference type="eggNOG" id="arCOG02287">
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.70">
        <property type="entry name" value="Hypothetical protein apc22750. Chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145_YbjQ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068:SF2">
        <property type="entry name" value="UPF0145 PROTEIN SCO3412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="UPF0145 PROTEIN YBJQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YbjQ-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="106" mass="12165" checksum="2D8436012299779D" modified="2011-06-28" version="1">MSQQQEIFVTTADEIPGYMVIQILGIVVGTSLLYKKFDLNKEEEAKKFLELSEKARLTAMDEMIKRAKEINSNAIISIRFDSHRINDEYDEIIAYGTAVKVIKNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B844</accession>
    <name>F4B844_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VapC-type toxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1996</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B844"/>
    <dbReference type="KEGG" id="aho:Ahos_1996"/>
    <dbReference type="eggNOG" id="arCOG00726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121774_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09873">
        <property type="entry name" value="PIN_Pae0151-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044153">
        <property type="entry name" value="PIN_Pae0151-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051619">
        <property type="entry name" value="TypeII_TA_RNase_PINc/VapC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901:SF1">
        <property type="entry name" value="EXONUCLEASE VAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35901">
        <property type="entry name" value="RIBONUCLEASE VAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="17"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="10740" checksum="69612E6B7C85B4AE" modified="2011-06-28" version="1">METLNAISNAKKKKVIDEEVANKLFEALNEFANAMKVYESKYYLEKAFKLAIKYGINTYSALYLALAEDLNLSLATLDAKQAKVASKMGIEVINIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B894</accession>
    <name>F4B894_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B894"/>
    <dbReference type="STRING" id="933801.Ahos_2047"/>
    <dbReference type="KEGG" id="aho:Ahos_2047"/>
    <dbReference type="HOGENOM" id="CLU_764198_0_0_2"/>
    <dbReference type="OrthoDB" id="42889at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="353" mass="42218" checksum="455603A7A102FF1E" modified="2011-06-28" version="1">MNTEKEKKIWEIIEKYPLILYLIDFNYLREITFKSTKIYNLLKEMENYIYPTREDDYVRCYFYLFLPVKVKNKLKFVPTGFCYLKESDEYEFFVHTKGGIKIGKGDEELPACYNSLIMRVYEFMKLQHQDPIFISTDDIYKHYLVGEVKLKYVTKPKMSNEEAKQILKQYKDNSKNKLPSDDITLRDYLEVVKIVYEANGLKVDKDLKELYKSYADGRDCGMIDLPLDDKEAFKIWRNSKAHCGGHPFEIIRGGFITYGVYLYPPREGRYTIIANDFIEEYINAVKEFLRRKIPFRAPDLIDVLKYLTGELIVKVNDYSDSPKHLFILYSEVENKRKIKWEEIREVNYRRRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D2</accession>
    <name>F4B8D2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl4</name>
        <name evidence="7" type="ordered locus">Ahos_0845</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775647.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D2"/>
    <dbReference type="STRING" id="933801.Ahos_0845"/>
    <dbReference type="GeneID" id="10600313"/>
    <dbReference type="KEGG" id="aho:Ahos_0845"/>
    <dbReference type="eggNOG" id="arCOG04071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="OrthoDB" id="10737at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_uL4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_uL4-arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="265" mass="29466" checksum="7ABE0AA93B901BFF" modified="2011-06-28" version="1">MYIQLQQKKADVIDLEGKKLKEIDLPLIFSYPVRKDLINRAFRSSFTKSLQPKGRDPMAGKRTTARSFGINLGLARVPRIRGSGEAALAPNTVGGRLAFPPSPMEKIVEEINKKEMKLAIISAISATAIKDLVKRRGHRLPDGLSLPIIVTDDLEKLSKSKDIIEFIEKLGLEDELERCKTKKIRAGKGKMRGRRYKIPKGPLFVIKDEKSPVIKAIRNIQGFDVITAKELSVIHLAPGGHPGRLTIFTEGAIQVLNERFGGELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D4</accession>
    <name>F4B8D4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl2</name>
        <name evidence="10" type="ordered locus">Ahos_0847</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775649.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D4"/>
    <dbReference type="STRING" id="933801.Ahos_0847"/>
    <dbReference type="GeneID" id="10600315"/>
    <dbReference type="KEGG" id="aho:Ahos_0847"/>
    <dbReference type="eggNOG" id="arCOG04067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_0_1_2"/>
    <dbReference type="OrthoDB" id="5987at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="9">
        <location>
            <begin position="11"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="8">
        <location>
            <begin position="87"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="238" mass="25477" checksum="956D96210945DA10" modified="2011-06-28" version="1">MGKSLLQQRAGRGNINFRNPGWLRVGKVRYPKIEGNHVGKVIDILHNPGMLAPVAKVKLDTGDVFYMQAVQGMTVGQKIEIGSNVNPATGNIVEVGNLPEGAIICNVEEHYGDGGRYARAAGSYATVIGKSGDRVLIRLPSGKIKEVLSNARATVGMVAGGGVYDKPMLKAGNVYWKYKVKATKWPIVKGVAMNAVDHPHGGGLHTSVSRPSTVSRNAPPGRKVGHIAARRTGRKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8D9</accession>
    <name>F4B8D9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 1</fullName>
            <shortName evidence="6">RNase P component 1</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnp1</name>
        <name evidence="8" type="ordered locus">Ahos_0852</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775654.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8D9"/>
    <dbReference type="STRING" id="933801.Ahos_0852"/>
    <dbReference type="GeneID" id="10600320"/>
    <dbReference type="KEGG" id="aho:Ahos_0852"/>
    <dbReference type="eggNOG" id="arCOG00784">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107020_3_0_2"/>
    <dbReference type="OrthoDB" id="39019at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="86" mass="9887" checksum="701569DCF96ADAC1" modified="2011-06-28" version="1">MKRPLDLIGLKVKVLSHSNPFFIGLSGVIVFESSKFLYIKSSKGKILMVYKADGIFEIDFKGSHQIMHGYKLMGNIVKRLKKRWTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E7</accession>
    <name>F4B8E7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl6</name>
        <name evidence="7" type="ordered locus">Ahos_0860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775662.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E7"/>
    <dbReference type="STRING" id="933801.Ahos_0860"/>
    <dbReference type="GeneID" id="10600328"/>
    <dbReference type="KEGG" id="aho:Ahos_0860"/>
    <dbReference type="eggNOG" id="arCOG04090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_2"/>
    <dbReference type="OrthoDB" id="7144at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_uL6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_uL6_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004037">
        <property type="entry name" value="PRK05518.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="181" mass="20426" checksum="C7B8E8096B20FD06" modified="2011-06-28" version="1">MLQVLVKESLKIPENVSLTLENSKIKVKGPKGEVEKDISHMRGIEVRLENGELIVEATFANKRTKSLVYTLLRHVQNMITGVTKGYRYYLKIIFTHFPMSVKVVGNEVQITNLIGEKNIRRAKILPGVKVTVKGEDIIVEGIDLEKVAQTAANIERASKISGFDRRVFGDGIYIYKKEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H0</accession>
    <name>F4B8H0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">[LysW]-L-2-aminoadipate/[LysW]-L-glutamate phosphate reductase</fullName>
            <ecNumber evidence="7">1.2.1.103</ecNumber>
            <ecNumber evidence="7">1.2.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lysY</name>
        <name evidence="10" type="ordered locus">Ahos_0883</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in both the arginine and lysine biosynthetic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="Rhea" id="RHEA-COMP:9714"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78501"/>
            <dbReference type="EC" id="1.2.1.103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl-5-semialdehyde)-L-glutamate + phosphate + NADP(+) = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52668"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="Rhea" id="RHEA-COMP:13327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:136761"/>
            <dbReference type="EC" id="1.2.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily. LysY sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.103" evidence="7"/>
    <dbReference type="EC" id="1.2.1.106" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775685.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H0"/>
    <dbReference type="STRING" id="933801.Ahos_0883"/>
    <dbReference type="GeneID" id="10600354"/>
    <dbReference type="KEGG" id="aho:Ahos_0883"/>
    <dbReference type="eggNOG" id="arCOG00495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_2"/>
    <dbReference type="OrthoDB" id="372053at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00037"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043870">
        <property type="term" value="F:N-acetyl-gamma-aminoadipyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23939">
        <property type="entry name" value="AGPR_1_C_LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17895">
        <property type="entry name" value="AGPR_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02083">
        <property type="entry name" value="LysY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050085">
        <property type="entry name" value="AGPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037535">
        <property type="entry name" value="LysY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338:SF11">
        <property type="entry name" value="[LYSW]-L-2-AMINOADIPATE_[LYSW]-L-GLUTAMATE PHOSPHATE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="9">
        <location>
            <begin position="3"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 8">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="349" mass="38525" checksum="83B64F0306721921" modified="2011-06-28" version="1">MVKVAVIGGSGYTGGELLRILSMHSKVEITLVTSREYAGKPISLVHPNLKGILNMNFSQLSLDKIGDKADTVFLALPHRVSLNYVPKLLEMGLQVIDLSADFRLKDPEEYKKWYGIEHPYPDLLKKSVYGLPELHYGELKGAKLIASPGCNATATILALAPVVGNGISTDLRFISDVKVSSSEGGMKPKEGSHHPERENAIRPYEPEGHRHAAEAEQELSLLAKKPVKVSIVPHAVSSIRGALASAHVWLNDNYDDIEIWKKIAEFYHGRKFIRIIRGGFHPYPDPKYVIGSNFADIGFAQEKRIMRLTMFAAIDNLVKGAAGQAVQNFNISRGFNEDEGLRTPPLRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8H2</accession>
    <name>F4B8H2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tetratricopeptide TPR_2 repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776839.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8H2"/>
    <dbReference type="STRING" id="933801.Ahos_2053"/>
    <dbReference type="GeneID" id="10601553"/>
    <dbReference type="KEGG" id="aho:Ahos_2053"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010547_0_0_2"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046813">
        <property type="term" value="P:receptor-mediated virion attachment to host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="190"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="258" mass="30242" checksum="550ECDD3B93C461B" modified="2011-06-28" version="1">MSQIDEIIKQYNSGNLNLALRKLEELVSKNPTKEAYELMAKILLEMGKDDEAMEYFEKAGNKEEKARILLSRGMYSEALEELKDVNSTQARIIRATIYLRKEDYRKVIEELNEINDELSSNPLYYKIKGIAEYYLGNYYDALRDLTRGIELYPLDSELYYYRALVKISLNEDKEAENDINTAINLNPYYAEAYFSKGLLKEKKGKYEEAIAYYSKSIDINPEYVEAYVRRAKAYMKSGMEKEAFEDIKVVRELKENKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I5</accession>
    <name>F4B8I5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetate--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_2066</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the autotrophic CO(2) fixation pathway. Activates 3-hydroxypropionate to its CoA ester. Can also activate propionate, and to a lesser extent acrylate, acetate and butyrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxypropanoate + ATP + CoA = 3-hydroxypropanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26534"/>
            <dbReference type="ChEBI" id="CHEBI:16510"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.36"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776852.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I5"/>
    <dbReference type="STRING" id="933801.Ahos_2066"/>
    <dbReference type="GeneID" id="10601566"/>
    <dbReference type="KEGG" id="aho:Ahos_2066"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043955">
        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043427">
        <property type="term" value="P:carbon fixation by 3-hydroxypropionate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF232">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="23"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="89"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="533"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="654" mass="73881" checksum="C70636B49CE0E54E" modified="2011-06-28" version="1">MVETQEISEQKLEEKADYNMRYYSYLFKLSKENPAKFWGDLAQELITWFEPWKETMKQEDPLTRWFIGGKLNASYNAVDRHLNSPRKFKAAIIWESERGERRIVTYQDLFYEVNRWANALRELGVKKGDRVTIYMPLTPEGIFAKLACARLGAIHSVVFAGFGSQALADRIDDAKAKVVITADAYYRTGKLVELKKIVDEALDRLGDKSPVSKVLVYRRTGVEIPFKEGRDVYFDEVGKYKYIEPERMDSNDPLFILYTSGTTGKPKGIVHSTGGYLVGTATMLLWSYGLSQENDVLFNTSDIGWIVGHSYITYAPLVMGRTVVIYESAPYYPYPDKWAEIIERYKATTFGTSATALRAFMKYGDDYVKAHDLSSLRVIVTNGEVLNYSPWKWGLEVVGGGKVFMSHQWWQTETGAPNLGYLPGLVYMPMKSGPASGYPLPGNFIEVLDDQGNPTKPRERGYLVMKPPFPPYMMMGMWNDPGNERLKKTYFSKFPGIYYPGDYAMIDEDGYVWVLGRADETIKVAAHRIGAGEVESAITSHPAVAEAAVVGLPDPVKGEEVHAFIVLKEGYTPSDELARNIQNHVREVMGPIVTPKIHFVDKLPKTRSGKVMRRVIKAVMMGSSIGDISTMEDEASMEEIKKAAEEFKKEVSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K0</accession>
    <name>F4B8K0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776867.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8K0"/>
    <dbReference type="STRING" id="933801.Ahos_2081"/>
    <dbReference type="GeneID" id="10601581"/>
    <dbReference type="KEGG" id="aho:Ahos_2081"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="13267" checksum="485813D34785815A" modified="2011-06-28" version="1">MLKPKTSADVYLEEAEDYLSKGEVVQACEKYYKAAEEAILILANKYKISNINEKDKASLERIVSLLSKILGEAIVKYWATAVLLLTAKDYMDQELVKIYKNDVERLVFIADNEGVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L2</accession>
    <name>F4B8L2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF973 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776879.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L2"/>
    <dbReference type="STRING" id="933801.Ahos_2093"/>
    <dbReference type="GeneID" id="10601593"/>
    <dbReference type="KEGG" id="aho:Ahos_2093"/>
    <dbReference type="eggNOG" id="arCOG03768">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1092385_0_0_2"/>
    <dbReference type="OrthoDB" id="374717at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009321">
        <property type="entry name" value="DUF973"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06157">
        <property type="entry name" value="DUF973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28878" checksum="B5E32C2E6F855AAC" modified="2011-06-28" version="1">MEKSPESRLKIVAILLAISTGAFYISYALVFYFIYVLKDNFFVEMIFPGTIGASLVTLLPIGFLTRGLRNTNFNIGYLMYVAGIILFEFIPIPFLIFLGNFFLGIAYRDIGRLEKNKKLENAGWVASVPFVSFIGFSLCYAYYKSPEEVEKEEEKRKEMEEIKPSPVPYQIGIGKMSSNGDFEFSFYSPREDKILNIKFEDKTINTEIPVKVGKNVVKSRVQIDPLKMVAGNLYNITITFSNGETFTAVVEYIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8P1</accession>
    <name>F4B8P1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioesterase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="GeneID" id="10601623"/>
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    <dbReference type="eggNOG" id="arCOG04331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119426_0_1_2"/>
    <dbReference type="OrthoDB" id="359228at2157"/>
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        <property type="component" value="Chromosome"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FlK"/>
    </dbReference>
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        <property type="entry name" value="FlK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
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        <property type="entry name" value="BLR0278 PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FlK"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fluoroacetyl-CoA-specific thioesterase-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="120" mass="13434" checksum="F8D78A08CA4C39E5" modified="2011-06-28" version="1">MEFLEKKFTVKPEHSAVKVSSGAVDVLSTPCLIAFMEDVSFTLLQEKLGKGLTSVGYHVDVKHLAPAPIGAEILVRSTIVKEEGKRVTFKVEAFYKDKKIAEGIHERVIVNEDEFMKRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8P8</accession>
    <name>F4B8P8_ACIHW</name>
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        <submittedName>
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        <name evidence="1" type="ordered locus">Ahos_2129</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776915.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8P8"/>
    <dbReference type="STRING" id="933801.Ahos_2129"/>
    <dbReference type="GeneID" id="10601630"/>
    <dbReference type="KEGG" id="aho:Ahos_2129"/>
    <dbReference type="eggNOG" id="arCOG05356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037618_0_1_2"/>
    <dbReference type="OrthoDB" id="39489at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050583">
        <property type="entry name" value="Mycobacterial_A85_antigen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098">
        <property type="entry name" value="ENTEROCHELIN ESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48098:SF3">
        <property type="entry name" value="IRON(III) ENTEROBACTIN ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="310" mass="36010" checksum="F3AD00813ED30155" modified="2011-06-28" version="1">MKYEIIKIESQELKDNYLKDPYVREVLVYTPDEIEEGLPLLIELAGINWSPNVNNRFHQIMTHLLKKMKAVVVNPNFRTKYYLNQYINSPAVGNYENFIIKELIPELKERYNVGNVALFGKSSGGFGAYTLAVRHPDVIQGFADHFGDSCFYYLYANDFVYTIKALEGKKPKDLLKELITKTPSEDDMKILNVFGSSAFYSPNLNSETSFDLPFDETGEIIDEVWKKWLSFDPVKNVENYVDSLRKLKAIYLDVGKCDEYNLFIGMRSLHKKLQKLGIRHYYEEFKGGHFGNSSRYCASLPYLYERLKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q1</accession>
    <name>F4B8Q1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0885</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775687.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q1"/>
    <dbReference type="STRING" id="933801.Ahos_0885"/>
    <dbReference type="GeneID" id="10600356"/>
    <dbReference type="KEGG" id="aho:Ahos_0885"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_3_2"/>
    <dbReference type="OrthoDB" id="14434at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053483">
        <property type="entry name" value="HTH-Lysine_Regulator"/>
    </dbReference>
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        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040947">
        <property type="entry name" value="trans_reg_LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF4">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="141" mass="16064" checksum="CD80B1A9B64F92EF" modified="2011-06-28" version="1">MSSKIDNNDLKILETLKKNARTPYTLIAKDLKISEAAVRKRIEKLIRLGIIKRFTIEYELENEIKAIVMVKSTPQIPTPEISKKIIKISGVETVYETTGDYDIITIVRGINIAEINKTIDEIRSIQGVISTTSTIILRTWY</sequence>
</entry>
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    <accession>F4B8S6</accession>
    <name>F4B8S6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/threonine protein kinase with TPR repeats</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0910</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775712.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S6"/>
    <dbReference type="STRING" id="933801.Ahos_0910"/>
    <dbReference type="GeneID" id="10600382"/>
    <dbReference type="KEGG" id="aho:Ahos_0910"/>
    <dbReference type="eggNOG" id="arCOG03682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_418989_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14014">
        <property type="entry name" value="STKc_PknB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="360"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="654" mass="74965" checksum="85AC25B71C74113B" modified="2011-06-28" version="1">MQFVFTDGNKKYIFNDGKVEEYFGKLKTKDNIIGYLATIDGDKIKLSKFPIYDCSKIIFEGRLTIKVGNLGYLFESNESLCLFLGNIDTPVINGNYLIIKGIVIVSGNKRKLLDSMDNYDVIQYALSKYPSDDEVIRQAIIGLSKLGKCSEAISLYTKLDKKYPEESLAVAECYEKIGEELEALKIYSFFSDERYKILEEKLRKKVDKIIEEYDATGNAKILYNALSILPTYDAPALKLGWHFIRKNPEESIKFFEEAVKRSRSYHNLLMLANAYIKSGKYMEALKIIEEAEKMRRTAGSAFLRGLALENLNAKSEAEKDFLYACKEGIVEACEKVKPGVLYSPRDFYPEQWIGYVIYGYEVKQVLGTGGMGYVLLVERLGKKYAMKIMKKEYNFDEMLNEVAKMQEISKNSKYLVRILANFIDENWVDYYSSPPAIVMEYMGGGDLRDVLAKDEYSTLRHSVLWPQVVSVIFSKLANAIITIHKEGYIHCDIKPSNILFTSPLPNYGEEALEALEKEHVIPKLSDLGSAVKIGTPVIHYTPYYAHPLQRFGQRASTEMDIYSFTVSLYASLTNNFPFPEWLERELEEAVTNPSKREQALKDFYSVDPRMDYVPEEFKDIIMRGLRGEITMEEIARDLKDIAITEYNIPEEVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U5</accession>
    <name>F4B8U5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF402 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0929</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775731.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U5"/>
    <dbReference type="STRING" id="933801.Ahos_0929"/>
    <dbReference type="GeneID" id="10600401"/>
    <dbReference type="KEGG" id="aho:Ahos_0929"/>
    <dbReference type="eggNOG" id="arCOG04307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044303_0_0_2"/>
    <dbReference type="OrthoDB" id="84798at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050212">
        <property type="entry name" value="Ntdp-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39159:SF1">
        <property type="entry name" value="UPF0374 PROTEIN YGAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="FomD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF402" evidence="2">
        <location>
            <begin position="277"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="412" mass="48525" checksum="CC24222EAE8FA6A8" modified="2011-06-28" version="1">MKIRIRGIYATALTYMLSKNFEIVQQSPQIAERFMQEIIREPADITFKDGEDKGSLIVIGSADNFENYLKEIFPYSIVWKSPVKLYSLIETQNCKYQNYDVEPCIEKGIVIKPPTDGRKIILSPPKAVSKFAMVWRGEGKTFFSEHIIPEERKRLLNISIKYNKKGYNVKWRSNAQFEKNYLLEKDLENLIFKYENDDFSKQGCDFYLINLSLEDKLELDKIRSLVVPTINFHHMLKLSRSREVDIAESGKDNVTQILESLIKDYMEIEHIKINGRRIFLKPGKVIEKRISDNGYFLLLRREISGNGYYDGLGIRKEEGDYDIMEIDSSKWHEVHNYYDKNGKLKGKYINISTPPELLDGKIRYIDLEIDIIIKNEDKFVVDEEKLEENKKYLSYKMLERIKKEIEYVMTAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V6</accession>
    <name>F4B8V6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycoside hydrolase 15-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775742.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V6"/>
    <dbReference type="STRING" id="933801.Ahos_0940"/>
    <dbReference type="GeneID" id="10600412"/>
    <dbReference type="KEGG" id="aho:Ahos_0940"/>
    <dbReference type="eggNOG" id="arCOG03285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028187_0_0_2"/>
    <dbReference type="OrthoDB" id="36362at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF13">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="1">
        <location>
            <begin position="247"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="601" mass="69862" checksum="8A776CB91D55DFF9" modified="2011-06-28" version="1">MTRSIILGNGRLTILADQNYLLRELYYPLSTDNHLHMGRIGIWVNNRFYWLHDLHPEIEYEEDTLSSKVEASVEGIKIKIKDAVDMAYEILSREVTIEGNKEVKVFFAWDYHIYGTEYGDTALYDPATDGIIHYKRDRWFLFTCDIPTYQYATGYKEVMGYQGTWKDCEDGELSNNPIAQGAVDSAISFKINTGTTFYCWLIAGYNYGDVRKKNEYVQSKSPRELIDRTEKFWRAWLIKAKDYDKVVRRSLLTIVANWQENGAIPASLDTDIMRFNKDTYNYVWHRDAAFASIALTLLGYQDFSRKFFTFSKNLLFNGFLFQKYTVDGFWGSTWHPWTTGYVPIQEDETALLIYALWIHFRKFWDVDFVKDLYRPMVKSAADFLSTYIDEKTGLPLPSYDLWEERRGVHFFTSAAVYAGLVSASKFAEFFGDEEYKDKYFSAAQLVRKGLDTFYQGDHFARMLGDKGFDKTVDASTVLGAMLVYDPMDPRVISNRKVVEEKLTVNGGIIRYEGDWYLKESDKPNPWFITTLWVAQQHIAEGNIEKATEYLNWVLKNSLPTGIIPEQITPSGNYPSVSPLVWSHAELVRTYYYLKNGFTKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W1</accession>
    <name>F4B8W1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0945</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775747.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W1"/>
    <dbReference type="STRING" id="933801.Ahos_0945"/>
    <dbReference type="GeneID" id="10600417"/>
    <dbReference type="KEGG" id="aho:Ahos_0945"/>
    <dbReference type="eggNOG" id="arCOG00323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_757837_0_0_2"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="2">
        <location>
            <begin position="141"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="340" mass="38709" checksum="8CB5681E8E7ED5EA" modified="2011-06-28" version="1">MQTYELKEEIVHFISGSGIYKETREIRVNRYLTRSGWVLNKIEGIEEEIKSHEECVSYISPAVKEWENLDEILSKLTDVNVTVKKVHRKINDCIEEKILNYVEYNGLKFAYSGKPSDLKAVADFLKMQSITMNERIWSLERMNVILDPEATAHLFHQFMNFLRGDNPRIKLGERISSEITVYDDPLNENLIGFSVFDDEGVRTQKKEIIGDGIVLEYLGTLTAKSGSPGNARGVLPLPDYFNLIVKPKDWGFQELIQDTKNGLIVLGVIRSEIVKNSIRLFPRNSMLIGSGGVIVREIAIPLQELTTIDAISKEVKSVYIDDYHGGIAPFIRLKARPIVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-03-15" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X5</accession>
    <name>DN7C_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-binding protein 7c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">7 kDa DNA-binding protein c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Aho7c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0959</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="37274" last="37274">
            <title>The archaeal '7 kDa DNA-binding' proteins: extended characterization of an old gifted family.</title>
            <authorList>
                <person name="Kalichuk V."/>
                <person name="Behar G."/>
                <person name="Renodon-Corniere A."/>
                <person name="Danovski G."/>
                <person name="Obal G."/>
                <person name="Barbet J."/>
                <person name="Mouratou B."/>
                <person name="Pecorari F."/>
            </authorList>
            <dbReference type="PubMed" id="27853299"/>
            <dbReference type="DOI" id="10.1038/srep37274"/>
        </citation>
        <scope>DNA-BINDING</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="3">Highly stable from pH 0 to pH 12.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Hyperthermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 7 kDa DNA-binding/endoribonuclease P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775761.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8X5"/>
    <dbReference type="SMR" id="F4B8X5"/>
    <dbReference type="STRING" id="933801.Ahos_0959"/>
    <dbReference type="GeneID" id="10600431"/>
    <dbReference type="KEGG" id="aho:Ahos_0959"/>
    <dbReference type="eggNOG" id="arCOG05888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929990_0_0_2"/>
    <dbReference type="OrthoDB" id="33867at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003212">
        <property type="entry name" value="DNA-bd_7a-e_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045555">
        <property type="entry name" value="Sul7d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02294">
        <property type="entry name" value="7kD_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000439047" description="DNA-binding protein 7c">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P61990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="27853299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="27853299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="60" mass="6878" checksum="89659271BB3E8857" modified="2011-06-28" version="1">MATKVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDDNGKTGRGAVSEKDAPKELLEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B904</accession>
    <name>F4B904_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776942.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B904"/>
    <dbReference type="STRING" id="933801.Ahos_2156"/>
    <dbReference type="GeneID" id="10601659"/>
    <dbReference type="KEGG" id="aho:Ahos_2156"/>
    <dbReference type="eggNOG" id="arCOG05957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2433664_0_0_2"/>
    <dbReference type="OrthoDB" id="33450at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="90" mass="10533" checksum="612128B8E1257F67" modified="2011-06-28" version="1">MIDKEKLGVNKLVHNTLSDCDLYVIEDKEGKTYLLFVFNNYFKIMPAYPGKWDCEESLYRPFGLFGFVFEGEDINEKIKKKLEELKSVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B932</accession>
    <name>F4B932_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776970.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B932"/>
    <dbReference type="STRING" id="933801.Ahos_2184"/>
    <dbReference type="GeneID" id="10601687"/>
    <dbReference type="KEGG" id="aho:Ahos_2184"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF6">
        <property type="entry name" value="TYPE IV PILUS RETRACTATION ATPASE PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="229"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="573" mass="64902" checksum="532D3BE5E59347E8" modified="2011-06-28" version="1">MKFSFPKLTFKNNQNKITVTKKINLPAPLMPISAQTEELTEIVSDYEINLLNIIPNDIKKTINDANIELSIANPHVFITYDQEKGIYKYVLLEPPMDYPAFDIYTFLISEIERRLVEESSTIDLGGILLASAAKRPDLKIIQGERAGLKLLSTRAKVALYYLLRNMFGYNVLTPLLGDFRIEDISCSGLDLPVFVYHREFEYIPTNITFQEKMKVLDMEVDGKELLDEMVLRLISLSGKTISIADPISDGILPKGDRIAATFRSEVSARGSSFVIRRFSERPITILDLINSGVISPDVAAYLWYAIDMRMSFMVIGVTGAGKTTVMSSILNLAKDSMKIVSIEDIPEIRLAQDNWVQLYARAAYGESGKEISLMDLLKLSLRYRPDIIVVGEIRGAEAYVLFQALSTGHGGATTFHAYDTDSAIKRLMNAPLNIPQEWIPMMNIVINVRRLPVYVGDKIILKRRVVGVDEIVSWNDFRRVVNWDAKSDTHILDIENAKVMRSRLEESGRTLQELKEELERRALYLKMLASARDVVQDPESYKLVKKYIIKYSIRPEEALAEVSRISSVKPTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B938</accession>
    <name>F4B938_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776976.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B938"/>
    <dbReference type="STRING" id="933801.Ahos_2190"/>
    <dbReference type="GeneID" id="25395620"/>
    <dbReference type="KEGG" id="aho:Ahos_2190"/>
    <dbReference type="eggNOG" id="arCOG05953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656773_0_0_2"/>
    <dbReference type="OrthoDB" id="38858at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="159" mass="19029" checksum="A82CBDF7A1DCE641" modified="2011-06-28" version="1">MSLRELQEDKWPGKFEITDDDIKEVEDFMLKIINGDSYYEDHKNNNENLHTLTYNVKEQFKKSLTDEILDKGYVIMNNQNISINAHPNLVKIPFDDERSIVTFKDTIELLLSLFSIYKEENIEDKIPKRLVPLFILLKRSGLIYFDWKDKKYKLTTNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B955</accession>
    <name>F4B955_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-pyruvoyl tetrahydropterin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776993.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B955"/>
    <dbReference type="STRING" id="933801.Ahos_2207"/>
    <dbReference type="GeneID" id="10601712"/>
    <dbReference type="KEGG" id="aho:Ahos_2207"/>
    <dbReference type="eggNOG" id="arCOG02172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_6_0_2"/>
    <dbReference type="OrthoDB" id="6529at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="136" mass="15571" checksum="482B9981B649AACE" modified="2011-06-28" version="1">MKVKIGIEGFTIDSAHYTLSSPGDSQLHGHTYIINVEVEGNINPANGFVIDFNKLKDEIGKIIKEWDHKLIVPKKDLEKISISGPFRNEIKIIEEDYPTAEYIGFELAKEIYNRINMPIKIKIYEGKDSYAIIEYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B957</accession>
    <name>F4B957_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Integral membrane protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0964</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775766.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B957"/>
    <dbReference type="STRING" id="933801.Ahos_0964"/>
    <dbReference type="GeneID" id="10600436"/>
    <dbReference type="KEGG" id="aho:Ahos_0964"/>
    <dbReference type="eggNOG" id="arCOG02175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_345038_0_0_2"/>
    <dbReference type="OrthoDB" id="42357at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050545">
        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF6">
        <property type="entry name" value="MEMBRANE PROTEIN YDGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="648"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="675"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="699"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="742"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="776"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane transport protein MMPL" evidence="8">
        <location>
            <begin position="280"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane transport protein MMPL" evidence="8">
        <location>
            <begin position="650"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="811" mass="92244" checksum="0A81AA3AC143FC3F" modified="2011-06-28" version="1">MKPSVFIIAVWILIALLLAPIAFNIQSHFVYSDSPFLTTQYQSVKVENILKEYFNYTKESELFVIINGSYNESLEKINASLHYLYDAKLITPYEYISQVNSSYMSFVNPIIKEYENNLSYAIELYHNLTVEREELLSNLSWFYYQLNVTFKEGHNVTPSSYVNIYEEYLEETHNPILAGVKTFNDPFILLFSINNYTNESLIKETLCTFSNYSYLIYKLTGKYFPLCALQNTTVYVLHEVESKIPPPPIRLCNFHRGNTWIFIVRVPDNESLTNVEEFMQNTPAIVTGHFAIYAQSAYYTQQDLKIIDLVTIILVGSLLVILLRALFPIIFLIFSAVIGIEIAYSFLYLATFAGYKIYYISGLVIPPIVFGITIDYSILFMYRYFEEVKKNPNNALRTAFRTAGKGAIFSGLSIAIGFSSFLLSPSPLLKNIGEALIIASLSALIPAILFNYTALKIVSLRLLSFPRKEVPNPIDVRQKYLRDVSEFSIKHKFYVMGIMVILALASFAVFFTHTTTVNFTEIVPSNSETVIGENILSNYFNYSVDYIIIKGNPNLTYDKIYNLSKEIIDCRGLAYGPASFGKFITRNLTYLENIYSSHNYSLIEAYIPFPVFSKGAICFTQKLINQGYMVGGSNANRIDIVNNTVSTYYSFTLPLTIILITAYIALVLRSIVVPIRLSLTLLVSSLVGVAVMFEVFKEVYWLSPLIVFALLFSLGIDYDMFIILRIKEEKGKDEDERIIKGITYTGLVVTAAGLILAGAFFSLISADMRFLEEIGFSVGFSVIFDTFIVRPIFVPAIMSILKKYNWWPFAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-03-15" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B991</accession>
    <name>DN7A_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-binding protein 7a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">7 kDa DNA-binding protein a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Aho7a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0998</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2016" name="Sci. Rep." volume="6" first="37274" last="37274">
            <title>The archaeal '7 kDa DNA-binding' proteins: extended characterization of an old gifted family.</title>
            <authorList>
                <person name="Kalichuk V."/>
                <person name="Behar G."/>
                <person name="Renodon-Corniere A."/>
                <person name="Danovski G."/>
                <person name="Obal G."/>
                <person name="Barbet J."/>
                <person name="Mouratou B."/>
                <person name="Pecorari F."/>
            </authorList>
            <dbReference type="PubMed" id="27853299"/>
            <dbReference type="DOI" id="10.1038/srep37274"/>
        </citation>
        <scope>DNA-BINDING</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="3">Highly stable from pH 0 to pH 12.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Hyperthermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 7 kDa DNA-binding/endoribonuclease P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775800.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B991"/>
    <dbReference type="SMR" id="F4B991"/>
    <dbReference type="GeneID" id="10600471"/>
    <dbReference type="KEGG" id="aho:Ahos_0998"/>
    <dbReference type="eggNOG" id="arCOG05888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2929990_0_0_2"/>
    <dbReference type="OrthoDB" id="33867at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003212">
        <property type="entry name" value="DNA-bd_7a-e_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045555">
        <property type="entry name" value="Sul7d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02294">
        <property type="entry name" value="7kD_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000439045" description="DNA-binding protein 7a">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P61990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="27853299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="27853299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="61" mass="7037" checksum="DB162E5A9E2FE932" modified="2011-06-28" version="1">MTTVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDDNGKTGRGAVSEKDAPKELLEKLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9D9</accession>
    <name>F4B9D9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type I phosphodiesterase/nucleotide pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9D9"/>
    <dbReference type="STRING" id="933801.Ahos_2214"/>
    <dbReference type="KEGG" id="aho:Ahos_2214"/>
    <dbReference type="eggNOG" id="arCOG01378">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039939_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="397" mass="45021" checksum="C5BF4D107B454144" modified="2011-06-28" version="1">MPANIIAIIAPYNRISSIYQLFYKDIIKIMHMSLELPNYEKNIYSLACGIADFLGVKRNCLSKLNISGKRLALVLLDGFGWNIMNRAGVVKKEAEKFQTVFPSTTSTVLTTLFTALTPGEHGILGYNTFVKRLGGIINTLKYTHPSIDERDSIQEAVPFEKAFPEVKSYLAEVKERKTIEIVPKGISNTQFSNATHGKTSETKEYTDIWDAIYAFSQVLQQNSYDFIYLYIPDVDTLAHKYGPYAEPTLNAARDIFESVYNVSTKLANVGNYTIVITADHGHVEVANNVNVKDDKEFLSMLEIPPYGDSRALFLKSRYDMKTYLYHRFNLRVFTKDEFPTLFGRIGNVELPDYIAVPLDNTAYIFDYKENGEYGKLKGHHGGLLQEEFEIPLVMING</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I6</accession>
    <name>F4B9I6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-cone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2261</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777047.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I6"/>
    <dbReference type="GeneID" id="10601767"/>
    <dbReference type="KEGG" id="aho:Ahos_2261"/>
    <dbReference type="eggNOG" id="arCOG03714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2406518_0_0_2"/>
    <dbReference type="OrthoDB" id="39386at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="4">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="10884" checksum="3A6DA8097D415496" modified="2011-06-28" version="1">MVKVIKRDGSEEELIYEKIVVSILKTGAPIDIARKIAFMTIGKIYMDNKESVTAKELTSLILNYLKAENETWYRNWIAYDRVAKKRETEKELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9W2</accession>
    <name>F4B9W2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777093.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9W2"/>
    <dbReference type="STRING" id="933801.Ahos_2307"/>
    <dbReference type="GeneID" id="10601815"/>
    <dbReference type="KEGG" id="aho:Ahos_2307"/>
    <dbReference type="eggNOG" id="arCOG00759">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_904936_0_0_2"/>
    <dbReference type="OrthoDB" id="34620at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="297" mass="33447" checksum="525B1AF4DAC54CE1" modified="2011-06-28" version="1">MVNIIIGGGITGLFTAVKISDAMIIDEKENLRNSNASLWSIIPPLCGKYFNECLKGEDDYVKIANEYGIYFRKTFLLRKSEKPLGGGKMMDKKEIKEIEPSIDLEYAEYFDNSLFIDGDELLQKIGKEFPFLHAKVTKIDVKDNYVESIETSAGVLKGENYIITASYLLSDLIGNYVKLIPFKGHLIIAEPYKKLNGIVVIGDRIAVQGKKMYLNGDSKQDSSMDIDFEEVKKTISTFSELINKSNLEIRVGFRSVSENNEPVIIKPFKNLMIVGGYRFGYAIAPYLAEKVKEMLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA00</accession>
    <name>F4BA00_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777131.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA00"/>
    <dbReference type="STRING" id="933801.Ahos_2345"/>
    <dbReference type="GeneID" id="10601853"/>
    <dbReference type="KEGG" id="aho:Ahos_2345"/>
    <dbReference type="eggNOG" id="arCOG01791">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069129_1_1_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.110">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="2">
        <location>
            <begin position="45"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="243" mass="28724" checksum="48ECDF1F976517DE" modified="2011-06-28" version="1">MSEIFPGIFESDFYINEMIKLWDEGEKWAKWLDELAKRYNLERKVLDVPCGIGRVSYFLNKLGYEIVGVDISEKMIKMAKNNVPNGRFYRADMRYLKDVLNGEKFDLVINIFNSLGYYSEEDDLEILKSIKEATNKIAVINLDNRDYIIYNKPEVYYTYVHPYLVEDHNYFDPYTSRLKIRRTYMNQKGEIIGSIEYTQRFYSLHEIVNLVKKAGFKILDVVTAYSWKKFEVTDPQITIIAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA20</accession>
    <name>F4BA20_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dihydroorotate dehydrogenase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775933.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA20"/>
    <dbReference type="STRING" id="933801.Ahos_1133"/>
    <dbReference type="GeneID" id="10600607"/>
    <dbReference type="KEGG" id="aho:Ahos_1133"/>
    <dbReference type="eggNOG" id="arCOG00603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_0_1_2"/>
    <dbReference type="OrthoDB" id="36608at2157"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053488">
        <property type="entry name" value="DHODH_Type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041011">
        <property type="entry name" value="dihydoor_dh_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="2"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="290" mass="31478" checksum="4D2C4CB4E6ACE6C0" modified="2011-06-28" version="1">MRIANLDFKDPLIIASGIIPDIPEVMMRICENYEPSAITTKTLTLTPLEPHKPPTVIKFHDGCYMNAIGLGNPGIGIVEKINLKKCRLFVSIGGNDAKQIVNSAEIAENKADIIEINASSPNRKGYGESISSIVHEIVKEVKSHVKKPVFVKIGPWDNALEIAGKALEAGADGITAVNTLKGLMIDTEEFKPVLSYGTGGISGKCIYPLALRIIKDLYREYEPDIIGIGGVFSFREVLGMLSVGAKLVGLGSVILDKGFDVIGEIRKDLMNYLNEKGLKLEDIFAIAVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA25</accession>
    <name>F4BA25_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-directed RNA polymerase subunit M, TFIIS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775938.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA25"/>
    <dbReference type="STRING" id="933801.Ahos_1138"/>
    <dbReference type="GeneID" id="10600612"/>
    <dbReference type="KEGG" id="aho:Ahos_1138"/>
    <dbReference type="eggNOG" id="arCOG00580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166550_0_0_2"/>
    <dbReference type="OrthoDB" id="37175at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase II subunit RPB9-like zinc ribbon" evidence="1">
        <location>
            <begin position="21"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="119" mass="14051" checksum="5CC7D74D82033A29" modified="2011-06-28" version="1">MQKPYILYDVLIYLYGVLNMQFCPKCGGVMVPVKKDGKEVLKCKKCGYEKEMSTKDKKAYEIKETAKNNKVLTTSIVSEKEGRKRDEEEWEQEREEYYKEIGLELLRDEFEGSEENDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA50</accession>
    <name>F4BA50_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acetolactate synthase</fullName>
            <ecNumber evidence="13">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ahos_1164</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="13">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="13"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775963.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA50"/>
    <dbReference type="STRING" id="933801.Ahos_1164"/>
    <dbReference type="GeneID" id="10600638"/>
    <dbReference type="KEGG" id="aho:Ahos_1164"/>
    <dbReference type="eggNOG" id="arCOG01998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_2"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005039">
        <property type="entry name" value="PRK06456.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="197"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="394"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AEE94047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="572" mass="63452" checksum="7CB081BBEC3E3C17" modified="2011-06-28" version="1">MPSGARILVEALKREGVKVIFGIPGLYNMPFYDELYYEIQNQEIRHVLMRHEQGATHAADGFARVSGFPGVVTATSGPGATNLVTGMITAYWDSSPVIAITGQVNRSSIGKMAFQESDQPGIFKDITKYVVQLKTVSEIPIWIKNAFYIATTGRPGPVLVDIPRDVQLEKMDEVEWPEKPMVRGYKPFRTVIEPEKLKKAAEILVNAEKPIILVGTGVTWSNATTEVLNLAETLICPIISTLPGKSAIPHDHPLYVGPMGYYGRAEASLAALESDVMLIIGARLSDRTFTSYDEMIETKKKFIMINLDPTDTERAFKVDVAMYGDAKILTRELLNAVIKVGMKKDRSAWMKRVKELKEYYAQFYYQDEPGKIKPWKALKTIRNAIPRDAIVTTGVGQHQMWAEVFWEVLEPRTFLSSTGMGTMGFGLPAAMGAKLARPNKVVVDLDGDGSFMMTGNNLATAVDEHIPIISVIFDNRSLGLVRQVQDLFQNKRIVGVDYGPSPDFVKYAEAFGALGFNADSYEELEKAIKTAIREDMPTVIRLPIDKNELALPTLPPGGKLKQVIVSDPRKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA61</accession>
    <name>F4BA61_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">A-type ATP synthase subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpB</name>
        <name evidence="12" type="ordered locus">Ahos_1175</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The B chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775974.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA61"/>
    <dbReference type="STRING" id="933801.Ahos_1175"/>
    <dbReference type="GeneID" id="10600649"/>
    <dbReference type="KEGG" id="aho:Ahos_1175"/>
    <dbReference type="eggNOG" id="arCOG00865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_2"/>
    <dbReference type="OrthoDB" id="32941at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="10">
        <location>
            <begin position="13"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="9">
        <location>
            <begin position="136"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="11">
        <location>
            <begin position="359"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="466" mass="51450" checksum="DAC13C9A8044F55E" modified="2011-06-28" version="1">METTLNVREFSNISMIKGPLMVVKGVTDAGYNELVEIEMENGEKRRGIVVDSQLGMAVVQVFEGTTGISPTGTNVKFLGRGLEVKISDEMLGRVFNPLGEPLDNGPPVISGEKRDINGSAINPAVRDYPEEFIQTGISAIDGLNSLLRGQKLPIFSGSGLPANILAAQIAKQATVRGEESNFAVVFAAIGIRYDDALFFRKFFEETGAINRVAMFMTLANEPPVVKILTPRTALTLAEYLAFEKDMHVLAILIDMTNYCESLREISAAKEEVPGRGGYPGYMYTDLATIYERAGRVKGKKGSITQMPILTMPNDDMTHPIPDLTGYITEGQIVLDRSLYNKGIYPPINVLMSLSRLMKDGIGEGKTREDHKDLANQLFAAYAKAVDTRGLAAIIGEDSLSDTDRKYLLFGDAFERKFVNQGVNENRDIETTLDIGWEILSILPERELTNIKEAYIKKYHPAYRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA73</accession>
    <name>F4BA73_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775986.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA73"/>
    <dbReference type="STRING" id="933801.Ahos_1187"/>
    <dbReference type="GeneID" id="10600662"/>
    <dbReference type="KEGG" id="aho:Ahos_1187"/>
    <dbReference type="eggNOG" id="arCOG03704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06041">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_363570_0_0_2"/>
    <dbReference type="OrthoDB" id="28630at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053108">
        <property type="entry name" value="Chlamydial_TARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36975">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36975:SF5">
        <property type="entry name" value="TRANSLOCATED ACTIN-RECRUITING PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="47"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="743" mass="81196" checksum="548D066A9481F4DB" modified="2011-06-28" version="1">MEGKIVGFIGVIIILLIAAAVGFYEYSSVSSSYSSLSSSYSSLSSSYSSLNSSYSSLSAQLQKAEQNESSLATALKYAEQNETKYMNEAENYSQLYMMYKSLYKQNAEGAALDTVLEAYDGISIENPNDVLPYLAPNFTATIEGVPFPGTYNLESFNNTWLADFFSNYETVYFYTTALPTITQISHNTYEVTDVVQFFVAPTNDPIYLQVFNASNTIIVQDINGKMEITSLTWMGNELPPSAVIAGYPSQHTLESNVVLSEVLSEINGLGAEFNPATIAQNFAPNAVLEVTGQLPSVFMTGNYTGISNIENFFSTWDRYFIFVAEYSQNLLPNGTAVPPSVSVQLSTSGTMAVVVANVTPFIGFVNQGEPGFPNIYDIHVNLVTYLQYNSTTASWQIEKQIFNATMIPILQDTLYYNLNMPIFKVIGEDTVTVNASQGAILQEGNIVTVIKPGTYAMLPNGSMVSIYNFSLITFSLQAVMPPAQDFFNLTPTYAFAFAINGQISPKYALVNASGSPDPAITIVYAPDTWTSWTWFGGTFNGTAYIGGSYKFADHWIYGDGVIVNNQFFKPVLWIFESSCEPVGMPPTPVSVTPKEVFGLSPIADYTYEVNGITGGVVMAGNMLVVIQPGTTITTPSGTTLTTFNFSVVFYAPQNVTAPEPGQVPFLVFAYAINNCVSFHYVASKPFITVIMTPSCGANMWTWGLTKSGMYGYVLHDPILIGKGIVINLTFVRPVPWVLTLPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA81</accession>
    <name>F4BA81_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA81"/>
    <dbReference type="STRING" id="933801.Ahos_1195"/>
    <dbReference type="KEGG" id="aho:Ahos_1195"/>
    <dbReference type="eggNOG" id="arCOG01548">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="6">
        <location>
            <begin position="131"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="400" mass="45471" checksum="BA21E00F87B1B54A" modified="2011-06-28" version="1">MTSLDNYGMEVEVVPVQGELNVGPQHPGSGHMRILVKLNGDIVEDCELDVGYVHRAVEKLGENRNYMHLIPLVERPAILDSIHMNMGYVMAVEKILNVDVPERALYLRSFAAEVNRIASHLYGLGILGIFIGHSTAFMWGFGDREVWLQILEALTGARVTNSYIIPGGVRRDLTPSIIEMTKKAIVYMRKKLKDWEKIFLKNPTIMDRLQNVGVMTREQAIEWGAVGPNLRASGVNFDVRKAEPYAAYSKLDFEIPVYKEGDGYARTLVRFEEMEQSMRILEQIIKDIPEGPILADRFLKQIPPIRMKKWVQGQGRIVLPGYYASFRPPRGEAATRVEASRGELFYYVVSDGSPKPYRLRMITPSYRAIYVFKNLCKGARYADIVSIYGSLDYFPPEADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3L6</accession>
    <name>F4B3L6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2203 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774822.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3L6"/>
    <dbReference type="STRING" id="933801.Ahos_0010"/>
    <dbReference type="GeneID" id="10599450"/>
    <dbReference type="KEGG" id="aho:Ahos_0010"/>
    <dbReference type="eggNOG" id="arCOG05495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137908_1_0_2"/>
    <dbReference type="OrthoDB" id="7857at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018699">
        <property type="entry name" value="DUF2203"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09969">
        <property type="entry name" value="DUF2203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016498">
        <property type="entry name" value="UCP016498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13712" checksum="75481694C5765472" modified="2011-06-28" version="1">MIYFDLNTANSLLPWIKEKIKELRQTRLQIEEALVKGEKEALSEGARKIDKIIKEITSQGIIIRDPDLGILDFPAVINGRPAYLCWKDGEEEISFWHYIEEGFAGRKKITGKEDILSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M0</accession>
    <name>F4B3M0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774826.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M0"/>
    <dbReference type="GeneID" id="10599454"/>
    <dbReference type="KEGG" id="aho:Ahos_0014"/>
    <dbReference type="eggNOG" id="arCOG07315">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1178149_0_0_2"/>
    <dbReference type="OrthoDB" id="34642at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26693" checksum="862B6520E1D2EB85" modified="2011-06-28" version="1">MKIEYAKNIFPKIIELLNRATDEITIATGGFSEDFIEAIINRAASGVQTTIITSDKNWARWLENQKISYKKDEENKIRKSYLRLRDKIIIYSRLRYIILIISISIVIVLLIRNVIHSLVTLISFIIIIIINIIQFIYIRNMIKAEESKLNMLNIEVQKTLDELTSTRNILAKKLNIIESSALSFSIVIIDNKSLLLSCNLNSKNSSDIVMYEEIQKNDAIKLISYITNLGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N2</accession>
    <name>F4B3N2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">KAP NTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774838.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N2"/>
    <dbReference type="STRING" id="933801.Ahos_0026"/>
    <dbReference type="GeneID" id="10599466"/>
    <dbReference type="KEGG" id="aho:Ahos_0026"/>
    <dbReference type="eggNOG" id="arCOG07313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_297497_0_0_2"/>
    <dbReference type="OrthoDB" id="36367at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="958"/>
            <end position="1002"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1011" mass="118014" checksum="9249E57225A67409" modified="2011-06-28" version="1">MTCNYRISETAADSKSLYDLYLCISSKTRYIDRLKFFRQIGWEIKRLESPRESPEIIAVLADWGQGKTSFFNIIEEALKSRKINIYKDSFVNILKKEKGDISFIKDYPVVLIDEIESGVDFAVYRQYQDYIRDFWISLKELANSKGNNIVYLSTTPSAYSKVFGVGGQINSLFPETYYSFIQRIKTIQIMPPSKLEFLAMIDCLLDYNEKDKEILQYLDLPYWVISQERRKYARFFNDILCKSFEFKDPIEQLFKEISIATDLNDEGETLRNLVIELENKMDKGEIRKFHKVLLSRIFTDKNLIIKELEGHTVKGFLVDFPSWVEVTKEYKIPQDIEDFLIFYSSSSEFDKSINVFVSSDVNKVIYEGINVDLSDVYKKLKVKSKGEAYALEWGYYESLVNTNVGGLIVDFKSREIKEKALKFVNDSLLDIEKEVDSFIIFLNEIGLKFNQKYVAKNIRIIECDIGNSINVNLVIYKPTGREESIIKEILNEYDGIIHGIIFLGKSNEDLSKYSIVQAEIELFTPIKRQLLYLLFSSLNPDLTRVRKDVLDIKLGELKREINDIMSKILQKVTIQQLPLTKGNKRPIQSLNWVLFSPSIYPDNYQNVFVKVNQIVNEKFRIFGSKQFHLEDIETSAALKDDIITYLSENGIINSKDGGEIIYYDDLAGQKIKDFSSTLVGYLRQKELNIENLIAEYIYYLSEIKNVKPKLIQITGKIFGKSPTVEFLLYSSVTTGEIINHVDDKDKLIKDLIEQVDRRIDEIKSEDLNEGYFITIKKRDAGIRSLKDMKDSIMEYYNQLKLAIKNGDYRNQLRLVFLILTLYSLFKEFINQTINAQNEITKIKSYMLAKLDVISKAKRMLGINEELEEEKEINEILDYLESGKILNEFYELIERTTKTKRNEELMNFIDTIKKISNNEDNDNLHLIIWEIYKASIDGTPLPFFDELKNTTIYRYSERLREIGSNLMRLQAEIESIEKINPEVNKLRQEINNQEKQIYDLIKTIKGELNEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T3</accession>
    <name>F4B3T3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber evidence="3">1.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1237</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776036.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3T3"/>
    <dbReference type="STRING" id="933801.Ahos_1237"/>
    <dbReference type="GeneID" id="10600714"/>
    <dbReference type="KEGG" id="aho:Ahos_1237"/>
    <dbReference type="eggNOG" id="arCOG00619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_398833_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43819">
        <property type="entry name" value="ARCHAEAL-TYPE GLUTAMATE SYNTHASE [NADPH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43819:SF1">
        <property type="entry name" value="ARCHAEAL-TYPE GLUTAMATE SYNTHASE [NADPH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate synthase" evidence="4">
        <location>
            <begin position="44"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamate synthase central-N" evidence="5">
        <location>
            <begin position="424"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamate synthase central-N" evidence="5">
        <location>
            <begin position="587"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="711" mass="76790" checksum="083BB66CFA8B80A7" modified="2011-06-28" version="1">MIIKAYLPLPKQTDSEFWTIDKIEHIRHLATTGKPYKILDKRRNSLRILDRIEFKNVEGKIVEKPLASTYLSFSGIEMTTPLYLGDMSYGALSGNPNIAIATAADLTGTLAGTGEGGLHPEVAKHKRIFVQWASARFGVDIDVLNAGLGVVIKIGQGAKPGIGGHLPGSKVTKAISMTRRIPEGIDAISPAPHHDIYSIEDLGQRIEALKEATGKPVFVKVAATNYIPYITSGVARMGADGIIIDGHGAGTGATPEVIRDNLGIPIELAVASADSVLKKEGLRDKFTIIAAGRISDATDAAKLIALGADIVSVGTAALIAMGCVMVHKCHIGSCPTALTSKIDGTRIFDIEFGVKTLVNFVNGFSLELANILDNLGLSSIQELKGRRDLLYGFGLSRDVLSILGIEGEAEELNPKLGELWNKRRKFHLHELINKGEPTITSMGSTAPPDVEKPARILDWLRSDGAQVTRPPIDPYREDIDTSFLLNKGKAYLSLPVIFNVIGAPKEIAESLSWASYALGSMVFVESTVPKYSEISFSNDGKGKINWSYEIEEGYYLALKSEDEIDDTLVNKGIPGFIIDEDVLNEDLELVVSDVDTKLKEAGIRNKFDIIAKSSKLRDSADVFKLVALGADAVIMPYQILEIAIGEGSKGNLKERAFNLISGMKKEIALMAGAAGVYSVQSSLTGNRELLRAVNLNSYIARRIRVKQAGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V0</accession>
    <name>F4B3V0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Ahos_1254</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V0"/>
    <dbReference type="STRING" id="933801.Ahos_1254"/>
    <dbReference type="KEGG" id="aho:Ahos_1254"/>
    <dbReference type="eggNOG" id="arCOG02044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_376262_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="454"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="18">
        <location>
            <begin position="64"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEE94137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="779" mass="85511" checksum="FA9043EF0B6731D0" modified="2011-06-28" version="1">MNIFIVSTSWIKFILIFIRKGLMQSTKTLTRILERTNIIDTLVVSGIALISILIRSLSTQYPLGINGFDSWYLFYNALLIAQAHGNWYAVPPDVHAWFPWGFFIELGDTIGLPFIVALLSLPFYSTYGVNAVYTVTLFLYVALAGLGVAAAYVAVSGLTKSRIGGFVAAAIIAVSPALTVKNIVGGLPKTSWGGVFVLFSIYLLNYAIEKKKPIYGAFAGIPLFLAEVSWGGSTYIDLSLLAGAFLLILLNRNNEETAKAFTLMAIVTAFLTSWAPNSIGFLSGLAHGLSLLIIGALLFLDLYLKNILPKEISDSRALLITASAVFIFVVAIVGLSFLGVTPIPSRYYAIVNPFYQVTVPIDKTVAEYIPQPITSMITDFGIALFLSIIGMYYLIRESNLAGLWLLVLGVISIYGTSEQPYLFNYTAYMIAPLAGAGVFYIIDKTKEYKAKIIPIFVLSLVGVSLVADAGAAITMSNVPNEIVTSASPYPIINYAWVSALDWINQKTCPHAFILSWWDYGYWEEVIGNRSVIAENNTLNGTQIKLMAEMFLNNETFAATVLEKDFHLYPYGNPNYTIPVYIIAYDAVTQVNTSSGEEWYIGYPPDLGGEFLGYTTSLGDIGKAMGAMTVIAGYPINEYLNTSLITSEISYLNSTLGSIAQYDPSVATELNELSSIIGQAYPLAWTPKAYNSLIVNMFVEALEGTGYPVIAPFSSKLEFISGLQTTPGTPFPEIHMVYFQPVYVALFSWVKGPNYETYIMVMIYQFVQPGTIIPECVYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B422</accession>
    <name>F4B422_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcription initiation factor IIB</fullName>
            <shortName evidence="7">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tfb</name>
        <name evidence="9" type="ordered locus">Ahos_0093</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774903.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B422"/>
    <dbReference type="STRING" id="933801.Ahos_0093"/>
    <dbReference type="GeneID" id="10599533"/>
    <dbReference type="KEGG" id="aho:Ahos_0093"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="106"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="106"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="200"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="8">
        <location>
            <begin position="200"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE92986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="291" mass="32363" checksum="2CA01DB34BA11ED3" modified="2011-06-28" version="1">MSSEVICPKDKIVFDANRGEYICTETGEVLEDKVVDQGPEWRAYTEEENRERSRAGTINLAVHDMGLSTYMDTRAKDRIKAMRLRKLQIRSRVGTSEQRNLVKAMTILERIIDNLGLPKAVKEEAAIIYRKALEKKLIKGRSIEEVVAASVYAACRKMNIPTTLDEISKATSANKKEIGKAYRLLLREDVTEVPASDPKYYVMKIASLLGLSGKVMTAATEIVERAKKAGITSGKDPASIAAAAVYIAANINGERRSQREISEVSGVTQVTIRNRYREIARELGIDIEGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B437</accession>
    <name>F4B437_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase molybdopterin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774918.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B437"/>
    <dbReference type="STRING" id="933801.Ahos_0108"/>
    <dbReference type="GeneID" id="10599548"/>
    <dbReference type="KEGG" id="aho:Ahos_0108"/>
    <dbReference type="eggNOG" id="arCOG00264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094953_0_0_2"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02109">
        <property type="entry name" value="arch_bact_SO_family_Moco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032:SF4">
        <property type="entry name" value="OXIDOREDUCTASE MOLYBDOPTERIN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43032">
        <property type="entry name" value="PROTEIN-METHIONINE-SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase molybdopterin-binding" evidence="1">
        <location>
            <begin position="21"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="200" mass="23448" checksum="DDF714E73C70B9F7" modified="2011-06-28" version="1">MQTQTPPGQKYIKRFIFYAALGVPEVDIQQYRLKISGLVENPIEYTYDQLLTMIDTRYTRDFHCVTGWSVKDVYWEGVKIKTLVEKAKVKDEAKWVIFYSLDGYTSVVPKEDALAEDSIIALRINGKPLSIEQGFPARPFMPHLYGWKSAKWLTEIEFTSKYVDGYWEERGYNERGNVWEEERFKGQGGRHHLPRRPILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B453</accession>
    <name>F4B453_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mn2+-dependent serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776077.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B453"/>
    <dbReference type="STRING" id="933801.Ahos_1278"/>
    <dbReference type="GeneID" id="10600759"/>
    <dbReference type="KEGG" id="aho:Ahos_1278"/>
    <dbReference type="eggNOG" id="arCOG01186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090895_0_0_2"/>
    <dbReference type="OrthoDB" id="35902at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="220" mass="26276" checksum="6A1E4D5250C89DC0" modified="2011-06-28" version="1">MKFEDVIKSLYLQSKLIKLEEKEYVIKCYSTSAGIKWYFISSFFKSYPYASSPRERMNREIDFFTRKWREIGTPKIIDMDYDTNCIMRDYIKGRPIETEEDFLMLGKAFKIIHENGLSLGDTKIENFIVNEKIYVIDAEQAIQTKEERYFAWDILVFLLFLSYKYINDLKNFEKMSRAFLNSYKPTQKEISYILDIRNINVLTLFPPMSLNLIKKIIAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B472</accession>
    <name>F4B472_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl10</name>
        <name evidence="6" type="synonym">rplP0</name>
        <name evidence="9" type="ordered locus">Ahos_1297</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B472"/>
    <dbReference type="STRING" id="933801.Ahos_1297"/>
    <dbReference type="KEGG" id="aho:Ahos_1297"/>
    <dbReference type="eggNOG" id="arCOG04288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_uL10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="Ribosomal_uL10_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003095">
        <property type="entry name" value="PRK04019.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="114"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="294"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="333" mass="36622" checksum="BDCAB6E54F4014C3" modified="2011-06-28" version="1">MSLTVQQHKIPQWKIEEVKELKEKIKQHKTVIIASLDGFPTDKLHEIRKKFRDTMEIKVSKNKLFERAAKESGIDVSKIEQYLTGTNAFIFSNKNPFEISLMLSKFKLKRYALPGDKADEEVVIPAGDTGIPAGPMISVFGKLKIPTRVQDGKIAITKDTVVAEPGQPIPPEIVPVLQKLGIMPVYVKLRLKVAYHDNLIIPGEQLELKLDEYKSQILESYKNALALGVEIAYPEAEILKITLGKAYRNAMALAGESGYITPESAQVVFSSALAKAYALAGAISGKVDLGIQVPTAQQAPAPAKEEKKEEKKEEEKKGPSEEEIAGGLSSLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B491</accession>
    <name>F4B491_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved archaeal conjugative plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776114.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B491"/>
    <dbReference type="GeneID" id="10600800"/>
    <dbReference type="KEGG" id="aho:Ahos_1316"/>
    <dbReference type="eggNOG" id="arCOG07284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150445_0_0_2"/>
    <dbReference type="OrthoDB" id="38300at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="16161" checksum="C5DCBD3490AA9512" modified="2011-06-28" version="1">MGKEIEIPFINAEGYSVPLIKVRMECPKSGEDVLIYALPDSGSRFSLINRRTLNECFDNLEDHYFDTIILTNLNLHTKRYKLKFHFVELNENFEIPVAIADFGVYEGVFPSLILGREDFFSRTMICFDKNAKLIIKADNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E5</accession>
    <name>F4B4E5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD-dependent pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774951.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4E5"/>
    <dbReference type="STRING" id="933801.Ahos_0141"/>
    <dbReference type="GeneID" id="10599581"/>
    <dbReference type="KEGG" id="aho:Ahos_0141"/>
    <dbReference type="eggNOG" id="arCOG01064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_5_1_2"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="378" mass="41955" checksum="1272507733968725" modified="2011-06-28" version="1">MNVLILGSGYAGLTVAHRIRGYSKTIDITIISRSAIVRENTIFPLLLTDEIKVEDTEFNAKEVMERKGVNFVEAEVEAVEDGEVKTSKGTFDYDYLFIALGGAYEENFEKIKGHEHAYMHHTLEGFLGLKKELKEAEDGVKIFVGNAKNSPIEGPSYQVALISEFLLRKKGIKGEVYLATQSPRGVFGPIPDPRISEIANKYFEKRGIRLLKGKYVTEIRKDKVVLSDGEEIEADIKSVLPALSAPKAVKEFTDDSGFIPVSFPSFSYNERIFALGDSAKGMVGPKTARSAMVSAENATYAFLKKIGIDVDKPYYSQGILCIMEGGDDGGIVRFDKNSKESVSFILFGKHYVTIKKIYSRLLVNNAFNVPYHASLPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K1</accession>
    <name>F4B4K1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl13e</name>
        <name evidence="4" type="ordered locus">Ahos_0198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775007.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K1"/>
    <dbReference type="STRING" id="933801.Ahos_0198"/>
    <dbReference type="GeneID" id="10599638"/>
    <dbReference type="KEGG" id="aho:Ahos_0198"/>
    <dbReference type="eggNOG" id="arCOG01013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179008_0_0_2"/>
    <dbReference type="OrthoDB" id="17872at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008914">
        <property type="entry name" value="PRK12277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9770" checksum="207B845BD6812255" modified="2011-06-28" version="1">MNPPTPMIKRPNYSFEYPHERKSTREGRGFSLGELKAANLTPEKARKLGLRVDKRRKSVHQENVDALKKYLDELNKEKSTQSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K7</accession>
    <name>F4B4K7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ordered locus">Ahos_1354</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K7"/>
    <dbReference type="STRING" id="933801.Ahos_1354"/>
    <dbReference type="KEGG" id="aho:Ahos_1354"/>
    <dbReference type="eggNOG" id="arCOG01432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042858_1_0_2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052684">
        <property type="term" value="F:L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006316">
        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009057">
        <property type="entry name" value="PRK12391.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01415">
        <property type="entry name" value="trpB_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF6">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500824">
        <property type="entry name" value="TrpB_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="30"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AEE94237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="380" mass="42037" checksum="E0FDCB9C419E6902" modified="2011-06-28" version="1">MPKEVLRQQFTVERFIKIPEEVREAYLNIGRPTPLMRAKRLEEMLNTPAKIYFKFEGATPTGSHKINTALPQAYFAKAEGVNHVVTETGAGQWGTAVALAARMYNMESTIFMVKVSYEQKPQRRTIMELYGGKVYASPTNLTEYGRKVLAENPSHPGSLGVAMSEAIEYALTNNYRYLVGSVLDVAILHQSVIGQEAMKQMDILGEEPDILIGCVGGGSNFGGFAFPFLGAKRGEKFIAVGSYEIPKFSQGDYMYDFPDSAGLLPMVKMITLGKDYVPPPIYAGGLRYHGAAPSLSLLIKEGIVEWREYSEPEIFEAAKLFAEAQGIVPAPESAHAIKAVIDEALKVKKNHERKVIVFNLSGHGLLDLPNYESMMKRLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4L5</accession>
    <name>F4B4L5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">Threonine synthase</fullName>
            <ecNumber evidence="4 11 12">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_1362</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4 11 12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776160.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4L5"/>
    <dbReference type="STRING" id="933801.Ahos_1362"/>
    <dbReference type="GeneID" id="10600849"/>
    <dbReference type="KEGG" id="aho:Ahos_1362"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_0_0_2"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000014">
        <property type="entry name" value="Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="15">
        <location>
            <begin position="61"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="218"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE94245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="393" mass="42431" checksum="9E9502C44AFA8430" modified="2011-06-28" version="1">MKCLDCGYETDINQKIITCPRCGGLLEIKVKLKGFSFSNLKGRGVWRYKDAIPGNYSKIVSINEGNTPLIPSRIYKQTFYKFEGANPTGSFKDRGMTVAVSSAVNTGYKVVAAASTGNTAASAAAYASRAGLRIYLVLPKGKVALGKLAQSILYGATILEVNGSFDIAMASVMRLYKDLGIVYPLNSFNPWRLEGQKTIAYEIAEEIGEPDNVIVPVGNAGNIYAIWKGFTELQEAGVISKIPRMIGIQAEGAAPIAKAIEKGLNEPEFFENPETIATAIRIGKPVNWKKAIKAIRSSKGTALSVSDQEIIDAQKKLAREEGIGAEPASAAALAGYEKAINEGIVDKDQKNVLILTGHALKDPDAMLRLDARRILINPEHIENIVLGEINDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M2</accession>
    <name>F4B4M2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-NAD/AcoX kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776167.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M2"/>
    <dbReference type="STRING" id="933801.Ahos_1369"/>
    <dbReference type="GeneID" id="10600856"/>
    <dbReference type="KEGG" id="aho:Ahos_1369"/>
    <dbReference type="eggNOG" id="arCOG01350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064691_0_0_2"/>
    <dbReference type="OrthoDB" id="56451at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039065">
        <property type="entry name" value="AcoX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011386">
        <property type="entry name" value="Put_ATP-NAD_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697">
        <property type="entry name" value="ACETOIN CATABOLISM PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40697:SF2">
        <property type="entry name" value="ATP-NAD KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016907">
        <property type="entry name" value="Kin_ATP-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="353" mass="38985" checksum="4CD2CDBC8FD0BED1" modified="2011-06-28" version="1">MKIGLIVNPYAGSGGRLGYKGSDGLYIENPEIENRVKRFLDVVNNNIEFVSPKFKMGEYFLKKFGKKYETINAGKEKSTREDTIISARLLSDITNLIVFVGGDGTARDVVEGSQSKIPILGVPAGVKMHSGVFALNPETAGILVNAYVEGMANIIEAEVLDIDEDAYRKGIYKVKLYYIAKTINFKGLLVNSKEEISSPSYELDEIAEYIIDNMEDSTFYIMGPGSTVKAIEEKLGYTTNFLSTDLFLGKRLIKSGVNYMELLQLTGELKIILTPIGGQGFLIGRGNQEIGPEILKKAGKDNIIVVSSKEKISRIDCLRIDSGDPNVDKLLQGIYKVIVGYDEFYTIKTCSMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4M4</accession>
    <name>F4B4M4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4M4"/>
    <dbReference type="STRING" id="933801.Ahos_1371"/>
    <dbReference type="KEGG" id="aho:Ahos_1371"/>
    <dbReference type="eggNOG" id="arCOG04266">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162442_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="13672" checksum="2677F42581E196B2" modified="2011-06-28" version="1">MEYLSSNKSVKMTEDEINKALAKAEKEAEKRDHKKIWIDKMLKSAKTYYKLCPYYDKKTSNCFLMLSSNDSNKKCNREGRYDNCPVFLAFLDNKYQEYTSKKKILPLDFLDLAQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N9</accession>
    <name>F4B4N9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein S30a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776184.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N9"/>
    <dbReference type="STRING" id="933801.Ahos_1386"/>
    <dbReference type="GeneID" id="10600875"/>
    <dbReference type="KEGG" id="aho:Ahos_1386"/>
    <dbReference type="eggNOG" id="arCOG04293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_209471_0_0_2"/>
    <dbReference type="OrthoDB" id="28200at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006817">
        <property type="entry name" value="PRK09336.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="51" mass="5798" checksum="39A8C10CEA265376" modified="2011-06-28" version="1">MPSHGSLTKAGKVRNQTPKIQPKPKSKEVPRVRNKKEFEKRVLKASKEKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R1</accession>
    <name>F4B4R1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1408</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776206.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R1"/>
    <dbReference type="STRING" id="933801.Ahos_1408"/>
    <dbReference type="GeneID" id="10600897"/>
    <dbReference type="KEGG" id="aho:Ahos_1408"/>
    <dbReference type="eggNOG" id="arCOG00874">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008213_6_0_2"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17926">
        <property type="entry name" value="DEXHc_RE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="168"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="401"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="544" mass="63276" checksum="95F384EF5BE8A297" modified="2011-06-28" version="1">MFSKTFYLTQWLDEDTFSKLLTFARFLGRENGVSEFVIDIERARKNRVRAEEIKETLKDVGVSLTEEEYRELQKLLPTYDIEFSINEGKLIITPHVYVMDIIKKSEGNIPIKYNRQEKVFETYPYYYYDLKELFIENGLTVKELNLEFKKFDFSLSVTLRDYQIEAIQKWKDNNYRGVIALPTGAGKTIIGIKAIEETKVPTLIVTFTREQMLQWKETIMKFSSIRPEVGLYYSNEKEIRQITLSTYQTAFRHVSELSDKFDLLIIDEAHHLPADKFREVALGILATKRLGLSATPFREDGRHEELFKLMGGIVYYKPVDELIKKGYLAPYDIVQVKVFLTTEERKKYLTLLNKFRVLSKNKKLKELIVLAKNGDNNAIEALKVYNEIKKVVNFAQNKMLKIEEILNKEKGKKILIFTQYVDQAEEIARRFNTLLISGKMSKNERKKILDTFKFMKSGILTLTTVGDEGLDIPDASVGIIVTGTSSRRQFIQRLGRILRPVNGKRAILYEIIVSGTQEEYQSKKRKEDSILDEVQISSEYSDDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T0</accession>
    <name>F4B4T0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PaREP1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775012.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T0"/>
    <dbReference type="GeneID" id="10599643"/>
    <dbReference type="KEGG" id="aho:Ahos_0203"/>
    <dbReference type="eggNOG" id="arCOG06952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850706_0_0_2"/>
    <dbReference type="OrthoDB" id="41636at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16960" checksum="11517494E8F67CD8" modified="2011-06-28" version="1">MPIRDEQTYAKMSYNDFKVAFERLKRGELKLSAWRAYKSLRRMLQSLAMKYDLHRKDTPCAVPKNCMIRVAEELEKYYNGITEMTREALRLFDEWKENYARREDIIKLLKELRFEWISSHLTKEEMNEIEEIKKELENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B507</accession>
    <name>F4B507_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B507"/>
    <dbReference type="STRING" id="933801.Ahos_1426"/>
    <dbReference type="KEGG" id="aho:Ahos_1426"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017896_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="188"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="355" mass="40610" checksum="56C1F518A91B70C2" modified="2011-06-28" version="1">MFVVEFTLYHHSFEVLGGSERVAIAVLNTLKSIGFKVKLVTLHIDVEKVKKWDKNFVMPDEIVVKKFPIKFGLYKALFTSMLTRPGNTFSTIGDITNADYSYIHFPWSLTDNLKIINAEDNEPYIKNRKMRTYFLPYKYVHRIFFSKSKSKLLVNSTWTGKILELSGYSYEVLYPPVEVEEYLKIKGNRDPKLVLSISRIAPEKNLENLFSVAKALKDFTFVLLGSSGRSKKYLEEVKSISGKLGNVKIVEDFTHDDIVNYLSKAKVYFHPKVNEHFGISIVEAMSAGLIPVVHKSGGAWLDIVKEGKYGFGYSNVEEAVNAIIEASKYENDFRDVSLNFSFDKFNERLSKILAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B511</accession>
    <name>F4B511_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcium binding protein SSO6904 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776228.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10600922"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcium binding protein SSO6904" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="91" mass="10630" checksum="B8DC9B1F6E30959E" modified="2011-06-28" version="1">MSVLDQEEFIQLRKFKGKADKEELQKILEEIEEQVNKGVSLRSSIIFTYANYVEEVKKNRDFYNLISTILEKYSPKLGVENVTELIINTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B538</accession>
    <name>F4B538_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776255.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B538"/>
    <dbReference type="STRING" id="933801.Ahos_1457"/>
    <dbReference type="GeneID" id="10600953"/>
    <dbReference type="KEGG" id="aho:Ahos_1457"/>
    <dbReference type="eggNOG" id="arCOG12521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182833_0_0_2"/>
    <dbReference type="OrthoDB" id="34673at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="234" mass="26792" checksum="0B30F3EAB5BF1ABE" modified="2011-06-28" version="1">MELIDTKKLLEGYKLKDGDSVIIDSDSLSIIKYFHGLKKINLIADDNFIFEALEIAGFLRERQVEISVNNFPPSYEPKLVRHKKLEFPITRSKGKGLTWKVSGVDFLPGDFVLGKDFPVSDERTGILGYLVNKKAVVIFDKSNGDYIEGKIVGKLDGDEEYLVRPNKWLTDLVVFKVTFKKDKAIVDKKLVFCRPLGSVFLPLNRRDIYNVLLKLKIRSSGYPVECYDYKNSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B547</accession>
    <name>F4B547_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyridoxal-5'-phosphate-dependent protein beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1466</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776264.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B547"/>
    <dbReference type="STRING" id="933801.Ahos_1466"/>
    <dbReference type="GeneID" id="10600964"/>
    <dbReference type="KEGG" id="aho:Ahos_1466"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_0_2"/>
    <dbReference type="OrthoDB" id="10138at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005035">
        <property type="entry name" value="PRK06450.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="50"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="337" mass="37177" checksum="F865664EC5D364B9" modified="2011-06-28" version="1">MKQVCMRCGKEREGLELRCKRCGGPFKVEVEDLPFSRSLRDNFPYIKSWISLGEWNTPMIKNGEIYFKLDFLNPTGSYKDRGSVTLISYLAENGINAISEDSSGNAGASIAAYGSAAGMLVKIFVPSTARGNKLKQIESYGAEVVKVEGSREEVAKAAENSPYYYASHVLQPQFRDGIRSLAYEIVRDLGWRAPDNIFLPTSAGTLLLGVFEGFRHMFNQGIIAKIPNIIAVQTEQVMPLCSKVKGIKYTPPEKVTSIADALVSTDPFLLPEMVEVIRKYGDCIVVNDEEILDSWKELARKGLLVEYSSATVYSAYKKSKPEGLSVLALTGSGLKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C8</accession>
    <name>F4B5C8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">N-acetylglucosamine-6-phosphate deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0331</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775138.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5C8"/>
    <dbReference type="STRING" id="933801.Ahos_0331"/>
    <dbReference type="GeneID" id="10599772"/>
    <dbReference type="KEGG" id="aho:Ahos_0331"/>
    <dbReference type="eggNOG" id="arCOG00699">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_2_2_2"/>
    <dbReference type="OrthoDB" id="24954at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="44"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="370" mass="41161" checksum="80860D1364C6184B" modified="2011-06-28" version="1">MKLTNVKIVTPYKTFLGTIEIEGGKIKRVYEGKEEGGENLQGMMAVPGFIDIHTHGIGGFDFTSWDSKEEFIKNLLEMKKIYLKHGVTTFLPTTVTLPRENLIEACKAIGEIQDDSMPGIHLEGPYISEKHAGAQDIRYIRKPDVEELKECFNASKGKIKTITFAPEKDDINFINGLLNLGIYPSVGHTDANYETASKAFLAGANRTTHIFNAMRPFHHRDPGVILASIRFSKYIEIISDFIHVDRDVVEFLIDVTGVNRIVAVTDSIIATDLRDGEYTLGKTKITVKEGKALTKEGRLAGSTLTMDKAFRNLASLRSLNEVVMMTSENPAMAVGLNDRGSLEPGKKADIVILDDELKVKKVYLNGEEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5D4</accession>
    <name>F4B5D4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type dipeptide/oligopeptide/nickel transport systems, permease, DppC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0337</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775144.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5D4"/>
    <dbReference type="STRING" id="933801.Ahos_0337"/>
    <dbReference type="GeneID" id="10599778"/>
    <dbReference type="KEGG" id="aho:Ahos_0337"/>
    <dbReference type="eggNOG" id="arCOG00748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_8_0_2"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729">
        <property type="entry name" value="OLIGO/DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729:SF1">
        <property type="entry name" value="OLIGO_DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="99"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="306" mass="33828" checksum="B4F174D3B7BC45FF" modified="2011-06-28" version="1">MAIVVSKQSTINKKRKIRVISTLLHYIKPLWKNRKARVGLLITIFYILLATVGPLIVPKPVIVNVNPNMIFLPPMLNPYYILGTGPIGCSILGDIVWGSTFILEVSFLAGLFNTLIGIAVGMIAGYKGGILDNILMSINDIMMTMPSLILILILATMIKTTNPFILAGILSISGWTGLARAIRSQVLLMKNLPYVEVAKVLDLGSFHIVFREIMPTIASYIAIHFILNVESALYASVGLYFLGVLPVNQYNWGVMISEALNSAAIYGTSAFWYLLWPSVFVVLFMVGLIFLSYGIDEISNPRLRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H0</accession>
    <name>F4B5H0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776309.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H0"/>
    <dbReference type="STRING" id="933801.Ahos_1511"/>
    <dbReference type="GeneID" id="10601011"/>
    <dbReference type="KEGG" id="aho:Ahos_1511"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705185_0_0_2"/>
    <dbReference type="OrthoDB" id="29061at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43129:SF1">
        <property type="entry name" value="FOSMIDOMYCIN RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="393" mass="42579" checksum="E194A20B762D80F3" modified="2011-06-28" version="1">MDVGRKLRILIITSLAHFNNDGTFLIFPLLIVYYSTVDRISLVILGAMSIIYTLLSGLLSPLIGDFADKHNIDSALLSLGIFLEGAAIGLFGISFMFPSIALIALGSTLLGIGQAFYHPIGGAILSRTFSKEAGRALGINGSLGSLGRSLMPSIVTLLILGLGEITGLLTVSAYMVLSAMIIFIGLKFYKRESIDVRKSKEKLEKVFYKFLLILGSIVFIRSMFITGTTTFVGEFVYDVYHSKELAGIFLTIGFLGSVLGQPAFGWLTERKGGRYAFIVSSLLTIVTFIGFLAFAENLIVSLSFYTIFTFSAFSSFPVLLGYVSQTFPKNFYTVANSYIWGIGVTVGGAAGNALITVLLGLRYTILSSFYVMLGLAIFSAILMPLIPKRVSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I3</accession>
    <name>F4B5I3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776322.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I3"/>
    <dbReference type="STRING" id="933801.Ahos_1524"/>
    <dbReference type="GeneID" id="10601024"/>
    <dbReference type="KEGG" id="aho:Ahos_1524"/>
    <dbReference type="eggNOG" id="arCOG07723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2613391_0_0_2"/>
    <dbReference type="OrthoDB" id="384654at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="8875" checksum="65A4B9F96762BE36" modified="2011-06-28" version="1">MAVVIKVSEDVRERLVKLAGELQAKTGKRVTLNDVIKYLLDTHEEINSEGIDKELEDLQIKMDKNSSMKVDEVVYGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J9</accession>
    <name>F4B5J9_ACIHW</name>
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            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="3" type="ordered locus">Ahos_1540</name>
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    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J9"/>
    <dbReference type="STRING" id="933801.Ahos_1540"/>
    <dbReference type="GeneID" id="10601040"/>
    <dbReference type="KEGG" id="aho:Ahos_1540"/>
    <dbReference type="eggNOG" id="arCOG06009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163847_0_0_2"/>
    <dbReference type="OrthoDB" id="43560at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="269" mass="29475" checksum="C4B91FC2EB3BAB6C" modified="2011-06-28" version="1">MSFNGIAGAVNEPGEIPWELVLLYFLVALAIAFLLGKRSGGLKAFNTLDLIYIGIGSAFAVAWEFYIAGFLDRFIPSNPFVSVGFWGRIIIVFIIAALVRKVGVGMLTLFIFDVLGDIFHYGFGGEPMYFIYETFTYGLFVDLMIAATGGRIFGLPNFAPSVTTKPPTQNIQKTTMQSAEEEEEDTPTRSTSITKSTFIVGAIEGAIIGILWAFPDPVFYDGFFRPFIEAKIVNWGYIYYHIYAYMPGDAIAGIIGAIIAIIVARAVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
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    <name>F4B5L1_ACIHW</name>
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    <organism evidence="1 2">
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        <lineage>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L1"/>
    <dbReference type="STRING" id="933801.Ahos_1552"/>
    <dbReference type="KEGG" id="aho:Ahos_1552"/>
    <dbReference type="HOGENOM" id="CLU_1269848_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="25485" checksum="75E1106AF38AF6D6" modified="2011-06-28" version="1">MNDKETVKLLQYHHLLYKNLYSNNFSETARLVIDEHLGSFKPNKVQIILQFFRNLKNGIIPAIARDLQGLTAILLIKKDEGYMTKLKKTKFADYLIAKLQDSQAFTITRSDENKKVTIFDDEKEFLDEFKEKHGLDNDQAFYELLSTTFAFSIWRTLINLSKERVTMTELNECFGVLQQFALLLGILKVSQCKAKVYVEFFPYFFKNEFMEMIEDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5M0</accession>
    <name>F4B5M0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1561</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776359.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5M0"/>
    <dbReference type="STRING" id="933801.Ahos_1561"/>
    <dbReference type="GeneID" id="10601062"/>
    <dbReference type="KEGG" id="aho:Ahos_1561"/>
    <dbReference type="eggNOG" id="arCOG10174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1072083_0_0_2"/>
    <dbReference type="OrthoDB" id="43854at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="28855" checksum="D82FCA3E3097C13C" modified="2011-06-28" version="1">MDQEKIFFTLTFLLILSSLVLIIYSAHVIRISHEMLTPPSARVVSVYLCNGKIIISLLLNNTTPYPITINGGKISIVQTNQTILVRPSTLAKEEIINVSAPLLPSYAYYSTIGIKGLICGNISQNKVYITFYCVAAIHIVSYVKVVHLTYFNGTANVTFFVSTPVNYTVNFIRYIQLANDNLSRFVAENLGEIYIGRSFPAGVHNFTVSIEFPIVFCSNLEKSYTYTFFTYLNLTCHYLDENVTKCLQIYYILRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5N7</accession>
    <name>F4B5N7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
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    <gene>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775168.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5N7"/>
    <dbReference type="STRING" id="933801.Ahos_0361"/>
    <dbReference type="GeneID" id="10599802"/>
    <dbReference type="KEGG" id="aho:Ahos_0361"/>
    <dbReference type="eggNOG" id="arCOG02218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175270_1_1_2"/>
    <dbReference type="OrthoDB" id="43128at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="76" mass="8944" checksum="7D050FBF4D09302F" modified="2011-06-28" version="1">MGKSVVISVRIPEELKEELEKYNIDEAEVIRRALINEVKKAKAKELEKEAKEIKEVLSKIPIEDVIKEIREDREER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S6</accession>
    <name>F4B5S6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0403</name>
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    <organism evidence="1 2">
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775207.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S6"/>
    <dbReference type="STRING" id="933801.Ahos_0403"/>
    <dbReference type="GeneID" id="10599843"/>
    <dbReference type="KEGG" id="aho:Ahos_0403"/>
    <dbReference type="eggNOG" id="arCOG04294">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122250_0_0_2"/>
    <dbReference type="OrthoDB" id="33816at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="188" mass="21943" checksum="09589A5148DB51F0" modified="2011-06-28" version="1">MITGFDEIDRIISPDRIVEFYSTDREILWLFYHRVIVLSSPIKVVIVGERGGIDPELIRRFREIFNVKGEIYIRRAFKAQDVKPTIEAMNEDMILVDPYHHRKLYGEIVSAIRNAGRVFIFSFMDREKEGSIFGLHSAHSLIRLERRSNKSFSFKIIKSVNTDEVEIPYSIWELFGKSKGEGLLTFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S9</accession>
    <name>F4B5S9_ACIHW</name>
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        <submittedName>
            <fullName evidence="3">IS607 transposase TnpA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775210.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S9"/>
    <dbReference type="STRING" id="933801.Ahos_0406"/>
    <dbReference type="GeneID" id="10599846"/>
    <dbReference type="KEGG" id="aho:Ahos_0406"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="OrthoDB" id="28414at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04762">
        <property type="entry name" value="HTH_MerR-trunc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1390:FF:000002">
        <property type="entry name" value="ORF1 in transposon ISC1904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="entry name" value="Resolvase, N-terminal catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051491">
        <property type="entry name" value="Recombinase/Transposase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048046">
        <property type="entry name" value="Transpos_IS607"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033518">
        <property type="entry name" value="transpos_IS607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36172:SF1">
        <property type="entry name" value="RESOLVASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="Resolvase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="2">
        <location>
            <begin position="55"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="189" mass="22086" checksum="D2049BC5B5D8DDFC" modified="2011-06-28" version="1">MLKPKEVCQKLGISYRTLQSYVKKGYIKPVILQSGKWRFREEDVEKLMGIVRKRKVILYARVSSQKNDLINQVKYLEENVKEYDQVITDIGSSLDMKRKGFIKLLRMILNNEVSKVVVAYPDILVRFGFEIIEEVCKAHNCKIMVLNKEDKTPEKELIEDLISILVSFSEKLQGMRKEKVKKCVEELKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5U3</accession>
    <name>F4B5U3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775224.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5U3"/>
    <dbReference type="STRING" id="933801.Ahos_0420"/>
    <dbReference type="GeneID" id="10599860"/>
    <dbReference type="KEGG" id="aho:Ahos_0420"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697573_0_0_2"/>
    <dbReference type="OrthoDB" id="57531at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17370">
        <property type="entry name" value="MFS_MJ1317_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="163"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="369" mass="40208" checksum="81205EABB9A435B8" modified="2011-06-28" version="1">MLTNRREVLKISFSAFFADLGYQAAVASFPIILVFYFNAPILIYGIAESLNYGGGSLMSLLGGYLADKYGRKKIGVIGNSLIVILSFTGLAVNYIQALILFMLGWWFRNFRTPARRAMVSEVTRENERSEAYGILHALDIGGATLAILYLTIALYLGIRATVVVLFTAIPIIISTILLAMVKAGGKGKPKIMVRKGWILVISTMFFAFSQYSFGFPIITTAEFTHKLYLATITYGIFLVSSALFGYVFGKLRLSDYKALAFLGYLLASLASLGFAFLSPLGLIGIYPLSALMGIAVASTETFEPTIISKLSKGEVGTSMGALSLGRSIGLLIGNTAMGFLYQISFSYAYAFAFIMSFIAFMIVITSLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V7</accession>
    <name>F4B5V7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sugar proton symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0434</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775238.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5V7"/>
    <dbReference type="STRING" id="933801.Ahos_0434"/>
    <dbReference type="GeneID" id="10599874"/>
    <dbReference type="KEGG" id="aho:Ahos_0434"/>
    <dbReference type="eggNOG" id="arCOG02685">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_5_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
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    </dbReference>
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    </dbReference>
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            <begin position="21"/>
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        <location>
            <begin position="26"/>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="487" mass="53239" checksum="C07FE06B779C8E73" modified="2011-06-28" version="1">MASQSAVQQILDQIDTRKLNKFYWIITILAAIGGFLFGYDTSVIAIASVFVPYHFTGLVYGYEIASASLGAAIGAIIAYFYTDRFGRKSLLIVDAAIYTAAAILSALTINGIMLLVMRTIIGIAIGADSAVATAYITEYAPKDKRGSLAIIQQWMITIGILGSYFVGSTVLFIAPSLAYTVDWRLILGLAAIPAIIGLVFRFMMPESPRWLLLSGQVDKLKASLRRFGVIVSDDLINRAISEVRYEESQKFDTATKRAFLVVALWIIFQQITGINVPFYYGPAIILQLHLFGSTSNPVYSEIYSVLAASILAVINTTATYIAFKYVDRIGRRTLAISAYIGMFASDLIGGILVMNGILVGALFAFAGFIIFFAYGVGGTGWLIQAEYFKTAVRGRMAAIIALLDWLANFVIIEVFPVMLSSVGLAGSMFIFSALDAIALAIVYFLLPETKGLSLEQVVKMFGETPVSQLRKGRELVLQKEEKEIARE</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
    <sequence length="117" mass="13643" checksum="FA8E22B85105ABF2" modified="2011-06-28" version="1">MSSGNNVKIISMTRKIQVLDRFAGYCPVCGKQTSQILAYVYRKEKMPIPLIFVTIRRYLPEFYGVICEECNTVLRATRHSKEIAKKYGKSRFTSKHKNALEQKTRIIEIKIIKNDQK</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1597"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AEE94478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="248" mass="29255" checksum="309A711DA724B2D3" modified="2011-06-28" version="1">MSYTNKFRKLEELPKNSYIEIHSKTLPKFRKDLIYLYHGIPSHYGMDFSRISWYYVPHIAYEIEFSESERIFDYCSFGFWSPFKRFDQIRKLKGSKKLVMSFNYLISDDKFIKDQCKKRLSYPLNIICNTPLVSSKNLKGLIKLKCAMLRLFNVKVIIKDYIPYEELVRELSECKAFVFFQETDAPSSGSMRLAAAFGVPVYAKDNLRAKDSQVIRYKRLNEINQLPKDKINIDDGLDYIISLIQYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Y1</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="AlphaFoldDB" id="F4B5Y1"/>
    <dbReference type="GeneID" id="10601099"/>
    <dbReference type="KEGG" id="aho:Ahos_1598"/>
    <dbReference type="HOGENOM" id="CLU_1709010_0_0_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="153" mass="18152" checksum="7415775CB12544EF" modified="2011-06-28" version="1">MLGKFDSKIVGLGESDEVYDLPFYWRIKYVKGKLSEIRFPHSLFDGILFFIDSRTRSFNHVSIELARISKPKTKLYLILLDKMALEEKEAYVKLLSDKFELIGDEKNILVLYNKKQKNKRFVNTIYVYHPNLAKDGITEYAKHLIYRLKKKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B630</accession>
    <name>F4B630_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="1" type="ordered locus">Ahos_1650</name>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776443.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B630"/>
    <dbReference type="GeneID" id="10601150"/>
    <dbReference type="KEGG" id="aho:Ahos_1650"/>
    <dbReference type="eggNOG" id="arCOG06017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121284_0_0_2"/>
    <dbReference type="OrthoDB" id="33325at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="178" mass="20354" checksum="2E9A2E42BCF7172B" modified="2011-06-28" version="1">MCDYRIITTDRPVKDSGKAIIVSRETFNKLTSDIYLKVMASDDREKLGLSKSYYYYILDSMKKLGLIEDNALAFKLILPFVKGEKELKFDDGIIYLNGKQIISIDMSSSKYACPTCPVFAECVYGIKRIAMSMKIKTQSIDSEIEARNERLPSKLWYSLIRGIVAKVLPKLDSINVYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B632</accession>
    <name>F4B632_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enzyme for biosynthesis of extracellular polysaccharides</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776445.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B632"/>
    <dbReference type="STRING" id="933801.Ahos_1652"/>
    <dbReference type="GeneID" id="10601152"/>
    <dbReference type="KEGG" id="aho:Ahos_1652"/>
    <dbReference type="eggNOG" id="arCOG05403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182726_0_0_2"/>
    <dbReference type="OrthoDB" id="8690at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050404">
        <property type="entry name" value="Heme-degrading_MO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34474">
        <property type="entry name" value="SIGNAL TRANSDUCTION PROTEIN TRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34474:SF2">
        <property type="entry name" value="SIGNAL TRANSDUCTION PROTEIN TRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="97" mass="11684" checksum="CA746057757EC625" modified="2011-06-28" version="1">MINVGLYYRVKEGHEKEFEETFSKVVSILKNSDIGFIDAKLYKRVDDPREYLIYSEWKDLESFKKFILSRDYKETTTYGKTILEGRPYHKIFQEINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B640</accession>
    <name>F4B640_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Urease accessory protein UreH-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B640"/>
    <dbReference type="STRING" id="933801.Ahos_0440"/>
    <dbReference type="KEGG" id="aho:Ahos_0440"/>
    <dbReference type="eggNOG" id="arCOG06008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_942587_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="31733" checksum="BAC7D46882ED4E9A" modified="2011-06-28" version="1">MLNPPQGLTLSEILIFSYILGLLHGITPDEHTWPITFSYAVGKYSTKGGMKAGFLFSLGFTIQRAFLTTLGFLGLAAIYKEYNLDGPVYFVVGIVMAIAGSYILKGKYVHLPFDELIHKRREGENLRDVPLKMTIVHGLIAGFGFGAYATIITFFLAPQVPGLIFAPLPGTMFGLGTMTMQIILGAIFGNIIKFKGLSEEDIRYIGIKTAGRVLYYGGIVFSIVGLLIIAIPIIDCWAIPTGNPIPNLDAINIGFLLVVGTVGTIGIASIIQAYREVKKKVEKNKAAQVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B655</accession>
    <name>F4B655_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphohistidine phosphatase SixA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B655"/>
    <dbReference type="STRING" id="933801.Ahos_0455"/>
    <dbReference type="KEGG" id="aho:Ahos_0455"/>
    <dbReference type="eggNOG" id="arCOG01992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084603_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101006">
        <property type="term" value="F:protein histidine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051021">
        <property type="entry name" value="Mito_Ser/Thr_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004449">
        <property type="entry name" value="SixA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00249">
        <property type="entry name" value="sixA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20935">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="152" mass="17109" checksum="0AD6D637AEF5D1B3" modified="2011-06-28" version="1">MRHGEAEPQIDGKDDKDRKLVKKGLKQMKRVANFLDDLDYTFDRVLTSPYIRAYQSAEAILDELGVDDKKIETFNELSPEKEPSAFIEKLKEMDNTTLLVVGHEPYLSSLIRAMTGGNVELKKGGVAIVDYNPVEGKGVLKMLLTQKVLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B691</accession>
    <name>F4B691_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="12">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cysS</name>
        <name evidence="15" type="ordered locus">Ahos_0491</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775295.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B691"/>
    <dbReference type="STRING" id="933801.Ahos_0491"/>
    <dbReference type="GeneID" id="10599931"/>
    <dbReference type="KEGG" id="aho:Ahos_0491"/>
    <dbReference type="eggNOG" id="arCOG00486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
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        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotidylyl transferase"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cysteinyl-tRNA synthetase class Ia DALR" evidence="14">
        <location>
            <begin position="352"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="12">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
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    <feature type="short sequence motif" description="'KMSKS' region" evidence="12">
        <location>
            <begin position="263"/>
            <end position="267"/>
        </location>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="206"/>
        </location>
        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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            <dbReference type="SMART" id="SM00840"/>
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        <source>
            <dbReference type="EMBL" id="AEE93379.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="467" mass="54519" checksum="D33FB8EC832E3894" modified="2011-06-28" version="1">MIRVFNTLGRKFEDFSTVENGIVKMYVCGPTVYDYVHIGHGRTFVAYDAIVRYLRLKGYNVIRIQNITDIDDKIINRAKETGKSWEEIVDYYSKDYLENLSLLKVKIDLHPRVTQHIKEIINFVQGLIDKGHAYVAPSGSVYFDVDTYPHYGELSNTKKDQWNQGEEFVKEKRHPYDFALWKAWKPGEPYWESPWGKGRPGWHIECSTMSTRYLGEQFDIHGGGMDLIFPHHENERAQTESLTGKPWVRYWMHVAFLNIRGEKMSKSLGNIITLKDALKKYGADVLRYWYLSSHYRSPIDFSEENLEQSKVSLQRLKDALAVLKDILKEGPKSYSKDEDIKVQREIIQKIKEFDEAMSNDFDTATALKKIHEIANIVFSKLQNTRDFLAASIALDGFRIFNEVFGVMDEEMGLAYERIYEVIDGIIEVRNLLRQKKMYDLSDQIRSILESHGIKVLDSKDKSTWRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E7</accession>
    <name>F4B6E7_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172999_0_0_2"/>
    <dbReference type="OrthoDB" id="42895at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="108" mass="12627" checksum="089B5F82928074D7" modified="2011-06-28" version="1">MSEELLKPGEREMIQSRSYLYDLLDKLNDILENKKEILEQKGIAPKLSVTLELITLNRLYLDVIYKTYWNQLLEVINELNAIPELKDDMVDVNADVEEIKKLKQEGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6J9</accession>
    <name>F4B6J9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ORC1-type DNA replication protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0521</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in regulation of DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775325.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6J9"/>
    <dbReference type="STRING" id="933801.Ahos_0521"/>
    <dbReference type="GeneID" id="10599962"/>
    <dbReference type="KEGG" id="aho:Ahos_0521"/>
    <dbReference type="eggNOG" id="arCOG00467">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025112_3_2_2"/>
    <dbReference type="OrthoDB" id="195574at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01407">
        <property type="entry name" value="ORC1_type_DNA_replic_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055237">
        <property type="entry name" value="Cdc6_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014277">
        <property type="entry name" value="Orc1/Cdc6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02928">
        <property type="entry name" value="orc1/cdc6 family replication initiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001623">
        <property type="entry name" value="PRK00411.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF31">
        <property type="entry name" value="ORC1-TYPE DNA REPLICATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22703">
        <property type="entry name" value="Cdc6_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="57"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="7">
        <location>
            <begin position="315"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="412" mass="46867" checksum="85B6593B80A42CED" modified="2011-06-28" version="1">MALPSDIINSSLNSPSVLKDGSKLNPDYIPERLPHREDKLKELTIAFRDIVNEPGKSSVRVVITGRTGTGKTVTARIFGKAFAEQVKNRGIKVEYVHVNCHRERTLYLITLKIAEQLKLSIPPRGLSSQEVFKIIHEYLERRNMYVIITLDEFDYFLNSSPPEDIYFIVRLYDELAVNVKRVSYIFIIRDLTTLSSLDKTVRDHIARDIIEFPPYKSNELYDILQDRVKIAFYDGTVSDDAVKFISDINGFDKGGSGNARLSIETLELAGRIADAEGSPIVTVEHAKKANSRLNEEASIILDELNYLELHPMLLVKALINLSKREKKESFPIGEVEDEYSRLCELLGVEPRRHTQVFEYMRRLKLMGIINTQQSGKGMRGRTTLLSLTFPASQELDDYVTKLIGALKNSEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T1</accession>
    <name>F4B6T1_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">CRISPR associated DNA-binding protein, Csa3</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T1"/>
    <dbReference type="STRING" id="933801.Ahos_1747"/>
    <dbReference type="GeneID" id="10601247"/>
    <dbReference type="KEGG" id="aho:Ahos_1747"/>
    <dbReference type="eggNOG" id="arCOG01446">
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    </dbReference>
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    <dbReference type="OrthoDB" id="97174at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010163">
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    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22662">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Csa3 N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="23823" checksum="DDE939604EA9A21E" modified="2011-06-28" version="1">MNILLVTIGFDEKFQIRALMRRFKEIDKVVIVGYFNEERAKKALESFINVINTAGVSYELLEVNPYSFDDLIVRISKFINKNKGNRLIVNLSGGMRILILGVILSFIINYVDAEIEVETEDFKTVVSIRLKDLMPISLSNDHINILKAIKEGYVSINSLHSYLKIPLSTVWRRVKELISEGLLTEDLQLTYRGKLLLKIYESNFSMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B723</accession>
    <name>F4B723_ACIHW</name>
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        <submittedName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0618</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B723"/>
    <dbReference type="STRING" id="933801.Ahos_0618"/>
    <dbReference type="GeneID" id="10600065"/>
    <dbReference type="KEGG" id="aho:Ahos_0618"/>
    <dbReference type="eggNOG" id="arCOG08308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2505107_0_0_2"/>
    <dbReference type="OrthoDB" id="40712at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="10216" checksum="1ADED60FFB5D8DB4" modified="2011-06-28" version="1">MNDIRDELLKILKKMDPNIVDDSLDIKFLQQYKNRYDVFGQFKDDKGVYEFALSFDTKGKIYRQHINMIQTLKLREELEKKLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B725</accession>
    <name>F4B725_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxymethylbilane synthase</fullName>
            <ecNumber evidence="5">2.5.1.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0620</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775424.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B725"/>
    <dbReference type="STRING" id="933801.Ahos_0620"/>
    <dbReference type="GeneID" id="10600067"/>
    <dbReference type="KEGG" id="aho:Ahos_0620"/>
    <dbReference type="eggNOG" id="arCOG04299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="OrthoDB" id="8042at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="7">
        <location>
            <begin position="217"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="292" mass="32729" checksum="2EB40E0A6B557485" modified="2011-06-28" version="1">MIRIAARNSQLSQVQVKVVSDYLNKLGYETEFIGVKTKADLFVNEPLYKLGKGVFEKEVNEYVIRGLADLAVHSMKDLTSNLSEELDILATVKRDSPYDVLVSKKDVFDLDSGSVIGTSSIRRKNFMTFLRPDILVKDLRGNIDTRIKKYTSGEYVGIIIAEASILRLNLNINYFKLNPIDFTPEANQGIIAVVGKKNDERTRKLLSELHDQETFNEAIAERSTMEIVGGGCHSPFGVYFEQIDDRLLGIASFSNGKKKITVTIERKGNPKDVGHELGKLLLGEMKNENLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B758</accession>
    <name>F4B758_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B758"/>
    <dbReference type="STRING" id="933801.Ahos_0654"/>
    <dbReference type="KEGG" id="aho:Ahos_0654"/>
    <dbReference type="eggNOG" id="arCOG05890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237967_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="186" mass="21780" checksum="25D9203F0C51CA54" modified="2011-06-28" version="1">MREVKRKPKIDKFLEYFREATGFSCNIEYDIEGNLPFSSYYIYVTRKLVELAINKCEIPMNENDVNETLEMIDDAMFDSRLIRALRKAQELNLPILYRDGEDPVPLSEKEVRINLLTSFPLPKPRFLESSLIHLAGILPVEISKDSSLIQFENGLWSSLYGLPYPMNNSWKWIWDLNWASLIELLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B766</accession>
    <name>F4B766_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps11</name>
        <name evidence="9" type="ordered locus">Ahos_0662</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775465.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B766"/>
    <dbReference type="STRING" id="933801.Ahos_0662"/>
    <dbReference type="GeneID" id="10600113"/>
    <dbReference type="KEGG" id="aho:Ahos_0662"/>
    <dbReference type="eggNOG" id="arCOG04240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
    <dbReference type="OrthoDB" id="12054at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000007">
        <property type="entry name" value="30S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_uS11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="132" mass="13947" checksum="6D5BC40A3AC7D438" modified="2011-06-28" version="1">MSSRREIRWGIAHIYASQNNTIISITDITGAEMIAKASGGMVVKADREKPSPYAAMLAANKAAGEALDKGIMAIHIKVRAPGGSGPKTPGPGAQPAIRSLARSGFIIGRIEDVTPIPHDSIRRPGGRRGRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B793</accession>
    <name>F4B793_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1838</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776628.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B793"/>
    <dbReference type="STRING" id="933801.Ahos_1838"/>
    <dbReference type="GeneID" id="10601338"/>
    <dbReference type="KEGG" id="aho:Ahos_1838"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="298"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="382" mass="44877" checksum="BABDBD0557BAC626" modified="2011-06-28" version="1">MARRGNKAIRATVSMKIAVSDSLLALVNNYVKALRFTLFWLKENVKNPEEKGVLSKVHEELYTRLKGEYNLPSKVAEDCYRDALSIYKSWFNNPKKGRFPRVYKPTVWLTPKASYNVDFNKMSVRITSVGELSILGYPRNLREYMSWRMREARLVIKGGKAFLKVVFEKEEENVEPKESVAVDINMAEVVVGKDDEHYVRIPTRLEEVHHWKSLAESLQRKYPRRWKENKKIIHRIHSFHLKARRIAEDFARKVGKWIVEIAEDFGANVIKLESLKNLIKNVDKLPKEFHDKFYLMQYRRLQYWISWQARKHGMVIEFVNPSHSSVSCPKCGKRMEEVGYRWFRCSCGYENDRDVIAITNLNGRGSLTLSTAPQMRDVRANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B794</accession>
    <name>F4B794_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1839</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="175" mass="20587" checksum="0C23BA6991EF39C6" modified="2011-06-28" version="1">MESKIPTIEKHRNAYVKIRIIESLDELALGLKLLKEGFSRNSANKVFLSWKAIISALTVMNLEKMPRNEKEKEWYYKSGFLAPTTVLKGISQRLEELGYNVNSLTSVALELHRYAYNGLYKGASEYYEREEAINDIIYLSKEIMKVIKEFFKEYWDEEIEKHYNLAEKELMSKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7A4</accession>
    <name>F4B7A4_ACIHW</name>
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    </protein>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7A4"/>
    <dbReference type="KEGG" id="aho:Ahos_1851"/>
    <dbReference type="HOGENOM" id="CLU_2091166_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="12765" checksum="B3C426FFDB41F693" modified="2011-06-28" version="1">MLSNNRIDMVWKELKDEVKKSVKELTDVGKEVGKSSLSAAAGITKDGIEIGKELTKSSIELAKSTEREIKDTTKVAVDSTKELIEDLTDGVKDAMKGPIREMNKLGDKDQGKQENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C5</accession>
    <name>F4B7C5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Quinol oxidase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1872</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776660.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C5"/>
    <dbReference type="STRING" id="933801.Ahos_1872"/>
    <dbReference type="GeneID" id="10601371"/>
    <dbReference type="KEGG" id="aho:Ahos_1872"/>
    <dbReference type="eggNOG" id="arCOG01238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_534881_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00919">
        <property type="entry name" value="Heme_Cu_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053628">
        <property type="entry name" value="Heme-copper_oxidase_sub1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041075">
        <property type="entry name" value="quin_ox_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="506" mass="55968" checksum="DFBE7B95842A1447" modified="2011-06-28" version="1">MKLYPKSTVGVALMYTGGALAWLAVLGIAALWFRTILLSPHICCPGIGEKVISPLYYFLVTMHGQVAMFIVVPDLTLAAAAYGLYKSKMSILHFKIMTIAFWLINLPLIFEFAGGPTTGWYEYPPLALQKGTWDMYGGLPDANLMIGLAYFMQVLNTLGAIIGGLTLFFDGYKTRQREGKIPIFAAYGMAFGGMFMPITIVALTGAALWYSLYFWFGVPVNPLTWVVLFWFYGHPVVYYVPFTVFGGLYTLIPQYAGRPLYSERWARWNIALLFTFGMLAWVHHLQTWPLPIAIRAFITPTTLILAAGSGLTVLNLGLTIFTSKGYNWKDPIGMASLIALIGFILAGLQALMLPENPLNVLVHNTYYIVGHFHMMIWTIIIVGFTAILLDMLKTKMGNADFSNLGRGLLTAGLTMWTAAALALGYVMSYAGYNGLIRRWDAYPVKFLPYMDAMTYLAVTMAASFVMFAIPILGTLLPIKTSAFWVGAETSTLPSTSLPGTMAKHDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D9</accession>
    <name>F4B7D9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aldose 1-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776674.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7D9"/>
    <dbReference type="STRING" id="933801.Ahos_1886"/>
    <dbReference type="GeneID" id="10601385"/>
    <dbReference type="KEGG" id="aho:Ahos_1886"/>
    <dbReference type="eggNOG" id="arCOG05361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052486_1_1_2"/>
    <dbReference type="OrthoDB" id="39584at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01081">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="258" mass="29035" checksum="9719C31D5200649C" modified="2011-06-28" version="1">MHIQKGDTEAEILDKGAYLYSFKVKSKDILLEGRERQTRGGMALLIPFANRIKGGEYYWRGKKYELPKNSEGNAIHGLVRDKMWDVESLKSDEVTLSLYLKDPGYPSPLFIKVKYSLDTSSLTTTISVKNEGEEAPLVVGAHPYFIVKGNWRIFPEKAKRLIMKDKIPTGEMEDFTITQGEYDDCFLLKGNVTLISDYSKVTIEKENMDFIQIYTGQPSAVAVEPMSGAPDAFHNGIGLITLKEGETATFTFKISVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7G8</accession>
    <name>F4B7G8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775489.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7G8"/>
    <dbReference type="STRING" id="933801.Ahos_0686"/>
    <dbReference type="GeneID" id="10600138"/>
    <dbReference type="KEGG" id="aho:Ahos_0686"/>
    <dbReference type="eggNOG" id="arCOG04224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157709_0_0_2"/>
    <dbReference type="OrthoDB" id="43304at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="116" mass="12988" checksum="7C2079D0D508C2E4" modified="2011-06-28" version="1">MSQDNSQNEKKVDIKSLVSVIPPASALKSREREPPKEKRVKVRKKPEVDEGTIIISNKLVSQMNIKNEVEISVKGKRLRLKVISQDGLQDIEVWANPSDMLKLGLGDNSTVTLRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H4</accession>
    <name>F4B7H4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="6">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thi4</name>
        <name evidence="7" type="ordered locus">Ahos_0692</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogen sulfide + glycine + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775495.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H4"/>
    <dbReference type="STRING" id="933801.Ahos_0692"/>
    <dbReference type="GeneID" id="10600144"/>
    <dbReference type="KEGG" id="aho:Ahos_0692"/>
    <dbReference type="eggNOG" id="arCOG00574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="OrthoDB" id="4240at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="61"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="264" mass="28395" checksum="21388987B58A8BF8" modified="2011-06-28" version="1">MQSIRIKQVNEVKISKYILKYTFEDWNNLVESDVVIVGAGPSGMTAAYYLAKAGLKTVIFERRLSFGGGIGGGAMNFHKIVIETPADEIIKELKIRYIEPEEGIFIIDSAEFMAKLATAAIDAGAKIIHGVTVDDVIFRENPLRVAGVAVEWTSTQMSGLHVDPLFISAKAVVDATGHDAEIISVASRKVPELGIAVPGEKSAYSEIAEELVVENTGKVAPGLYATGMAVCEVKSLPRMGPIFGAMILSGKKVAEEIIKDLRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I3</accession>
    <name>F4B7I3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775504.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7I3"/>
    <dbReference type="STRING" id="933801.Ahos_0701"/>
    <dbReference type="GeneID" id="10600153"/>
    <dbReference type="KEGG" id="aho:Ahos_0701"/>
    <dbReference type="eggNOG" id="arCOG05939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172986_0_0_2"/>
    <dbReference type="OrthoDB" id="42845at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058494">
        <property type="entry name" value="DUF8181"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26552">
        <property type="entry name" value="DUF8181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="110" mass="12707" checksum="BE3B323ED78B0DBB" modified="2011-06-28" version="1">MSDSNFSIYPKIIVEFENDLDKIRATTSDDAKKLIDLAYSLSLEMDKEVKKIFDEISSNISKETQEEIDRLRKNYADEREKKISEIKEKANKNLEKAVSEVLNAIKGAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I6</accession>
    <name>F4B7I6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775507.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7I6"/>
    <dbReference type="STRING" id="933801.Ahos_0704"/>
    <dbReference type="GeneID" id="10600157"/>
    <dbReference type="KEGG" id="aho:Ahos_0704"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096706_0_0_2"/>
    <dbReference type="OrthoDB" id="30795at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="242" mass="27322" checksum="5A3096D323869A5D" modified="2011-06-28" version="1">MDSNPLEDTLARVSRFASILGISRAELRIYSTLLLEGRMTARQLAEQLGISYTKIYSLLTKLEERGWIKRLGKKPAVYEAIPLRDLWASIKKMIEIKVDEFEKEFIEPLSSLLSPSSTYSITMIPSDKIKSTFYEILDEGNKVSIAISYPELLSEDIIQAIKAKSYTSPDLRVIIQSDINIPEISGLQIRKLSNMFGSGLITSTAVLLIIKNADKLSGLFSNHKYIVDIATVYFNHLWIQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L6</accession>
    <name>F4B7L6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">H/ACA RNA-protein complex component, Cbf5p N-terminal exon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775537.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L6"/>
    <dbReference type="STRING" id="933801.Ahos_0734"/>
    <dbReference type="GeneID" id="10600188"/>
    <dbReference type="KEGG" id="aho:Ahos_0734"/>
    <dbReference type="eggNOG" id="arCOG06042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174504_0_0_2"/>
    <dbReference type="OrthoDB" id="35866at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9786" checksum="366ECB04F1F8F54F" modified="2011-06-28" version="1">MIFYDFIYKIDNFCGYNNPWNIKKEVQTSDKYGFFADKRPIEELIKNSIINLDKPPGPTSHEVAFWIKKMFNLSKVGHGGTLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7M7</accession>
    <name>F4B7M7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NAD-dependent protein deacetylase</fullName>
            <ecNumber evidence="8">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cobB</name>
        <name evidence="11" type="ordered locus">Ahos_1901</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-acetyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-acetyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sirtuin family. Class U subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776689.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7M7"/>
    <dbReference type="STRING" id="933801.Ahos_1901"/>
    <dbReference type="GeneID" id="10601400"/>
    <dbReference type="KEGG" id="aho:Ahos_1901"/>
    <dbReference type="eggNOG" id="arCOG04248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_0_2"/>
    <dbReference type="OrthoDB" id="728at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01413">
        <property type="entry name" value="SIR2_Af2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01968">
        <property type="entry name" value="Sirtuin_ClassU"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
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    </dbReference>
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            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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            <position position="24"/>
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        </ligand>
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            <name>NAD(+)</name>
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        <location>
            <position position="31"/>
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        <ligand>
            <name>nicotinamide</name>
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        </ligand>
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        </ligand>
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        <ligand>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01968"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="27659" checksum="30363E63C32BD865" modified="2011-06-28" version="1">MEKVVELLLSSTYAIAFTGAGISTASGIPDFRGPNGLWKKYSPEIASIDYLKRDPKGFWEFYSQRMRGLFEARPNEAHYALAKLEKMGIIKYIITQNIDGLHQKAGSQNVIELHGTMQRSYCSSCFKQYDSREVLKMIDEGNLPPKCSCGGIIRPDVVLFGEPVKDFDLALRIAYEADLVLVIGSSLTVYPANLIPQIVKEERGGSLIIINADETPLDHEADVIIREPIEVALPRIVNEIEEELRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N3</accession>
    <name>F4B7N3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1907</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7N3"/>
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    <dbReference type="GeneID" id="10601406"/>
    <dbReference type="KEGG" id="aho:Ahos_1907"/>
    <dbReference type="eggNOG" id="arCOG03654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031607_0_0_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
    </feature>
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            <end position="257"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="24"/>
            <end position="418"/>
        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="439" mass="47171" checksum="9FC4F4FDBA6A6AC3" modified="2011-06-28" version="1">MNNQKPAEINKSKTKSKLSTSDLFFLSFGGQAPFISLLTFGTVMISLVGTQGAFAMLIATAVVALNGIVVNRLSSRFKRGGGYYTYAYYALTERLGTSTGWVYLAYALSYGGTLLAGGAYVLYSILRMLPFTPAMLLNQWLLALVVSGLASALVIAGVKASAKYAMVMSTAEMIALIALSVFFLRDSGWHFYNPFTVNLSPSLFEAVVFGLGIPTGYGSIAPLGYDASSKSIGKVAVAVVFYGGLLASFFFYSLGALNFTGNLVQYLLSDFGIVGAIILGFIALNDGTLGGMSYILANSRTLKAMSEDKVFPSILAKDVRGKPLYAEILIAGIFTSAVVALTNFMGLYGVFVILGGLAGIFNLFIHSSADLSLVRITVKRAKKHWWELSIGILATLVSTAVLIYSFTGFSPIMMNIFLGWIVLGFFYLEIKEMLSSTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W0</accession>
    <name>F4B7W0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">infB</name>
        <name evidence="11" type="ordered locus">Ahos_0751</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8 9">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775553.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W0"/>
    <dbReference type="STRING" id="933801.Ahos_0751"/>
    <dbReference type="GeneID" id="10600208"/>
    <dbReference type="KEGG" id="aho:Ahos_0751"/>
    <dbReference type="eggNOG" id="arCOG01560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_2"/>
    <dbReference type="OrthoDB" id="30957at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000152">
        <property type="entry name" value="Probable translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="7"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="80"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="597" mass="66243" checksum="AF7560B57039AC4D" modified="2011-06-28" version="1">MIYLPKLRQPIVVVLGHVDHGKTTLLDKIRGTAMVKKEPGEMTQEVGASFVPTSVISKIAEPLKNIIPIKLEIPGLLFIDTPGHELFSNLRRRGGSVADIAILVVDIMEGFQKQTYESIEILKERKVPFLVAANKIDRIPGWKPNENEPFLFSLQKQSPAVQKKLDELIYNLVIQLASENFNADRFDRIKDFTKYVAIVPVSGKTGEGIPELLAILAGLTQRYMQGKLKFVEGPAKGVVLEVKEDPGIGHTIDVIIYDGILHKNDTIILGGLNGIIVTKVRGILLPAPLQDMRLAKTGYRSVDEVSAAAGVKISAPNLEEALAGSPLYVAENESKIDEYKKLIEEELSKIRFAKDVKGIVVKADSLGVLESIVTALEKRGIPVRLADIGPITKRDIVEAELAGKEEEEYGVIAAFRVKPIGNLDTSKVKIIYNDIIYQLIDEVEKYINDVRERKKRRTYDSLILPGKIKILPGFVFRRSDPAIVGVEVEGGIIKPKFPLIKDDGRKVGEILQIQDNKKSLDKATKGMEVAISIKGSVMVGRQINEGDILYTDVPKEDLEILVNNYKDTITDDMKEVIQEIIKIKRVEDPLYGLGIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X1</accession>
    <name>F4B7X1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine monophosphate kinase, putative (ThiL)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775564.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X1"/>
    <dbReference type="STRING" id="933801.Ahos_0762"/>
    <dbReference type="GeneID" id="10600219"/>
    <dbReference type="KEGG" id="aho:Ahos_0762"/>
    <dbReference type="eggNOG" id="arCOG00638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046964_2_1_2"/>
    <dbReference type="OrthoDB" id="45909at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF0">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="1">
        <location>
            <begin position="29"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="314" mass="36366" checksum="831E08A9AC7D96A4" modified="2011-06-28" version="1">MKLKDIGEHEFIRRSLSKYLGHDIFYDVYTEDGFTYKIDGFQLSYFFSFMDYYDIGWKAVTATVSDIITSGSKPRIILSSIGVNSEMEDKQLEDLFYGIIDATEYYEAKYVGGDLNNSEKNGWIDIAGIGTKLCDIKSNPEEGDIVIISDEIGFTSEVFISYLNNFNIPMHEISKLRVKHPIVNKNILNFYKEFCNYIVSSTDISDGLLISLQNLSDRLNYSILIEEFNINPSVYENLRRYGYGVNDILKYSGEEFSSLFVVKQEKAKEMIEWLYHYNFSPKILGKLGKERENEIKYKGNKLAISGWDNFKGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X7</accession>
    <name>F4B7X7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">siroheme decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.111</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0768</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>siroheme + 2 H(+) = 12,18-didecarboxysiroheme + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="ChEBI" id="CHEBI:140497"/>
            <dbReference type="EC" id="4.1.1.111"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ahb/Nir family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.111" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775570.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X7"/>
    <dbReference type="STRING" id="933801.Ahos_0768"/>
    <dbReference type="GeneID" id="10600227"/>
    <dbReference type="KEGG" id="aho:Ahos_0768"/>
    <dbReference type="eggNOG" id="arCOG01628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049427_1_0_2"/>
    <dbReference type="OrthoDB" id="145939at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3460">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040523">
        <property type="entry name" value="AsnC_trans_reg2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053953">
        <property type="entry name" value="NirdL-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF1">
        <property type="entry name" value="SIROHEME DECARBOXYLASE NIRL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17805">
        <property type="entry name" value="AsnC_trans_reg2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22451">
        <property type="entry name" value="NirdL-like_HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="65"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase NirL-like HTH" evidence="7">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme decarboxylase AsnC-like ligand binding" evidence="6">
        <location>
            <begin position="234"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="323" mass="37494" checksum="188AD5ED0B9D9716" modified="2011-06-28" version="1">MQLDEVDKKLLMELEYHFPYSPTPFYEISRRLGISEDETIDRIKKLVDVEIIKRIGMYINFRAKGMDGALVAAQIPLENLEKYRRITLGIRELTHNFIRNHPKYNVWFVIKAENREKLDYNIKNLMEEVNAKDYIVLYSKKSLKLSVKYDIIRGISWSENNETTLPEKIPTAEELGISKELLKALSVPLPITKRPFKELAEKFNMTEDQLIDLIKELKDKHVVKDYGATLNGEKVGIKENAMLLIDTEDIEKACENIALHINEATHVVLRESNKPWDYLCYCMLHGKDKQVIFNAVKKVIGETDAKSYMLLFSLENLKPGIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z4</accession>
    <name>F4B7Z4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">secG</name>
        <name evidence="16" type="ordered locus">Ahos_0785</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 14">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775587.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z4"/>
    <dbReference type="STRING" id="933801.Ahos_0785"/>
    <dbReference type="GeneID" id="10600245"/>
    <dbReference type="KEGG" id="aho:Ahos_0785"/>
    <dbReference type="eggNOG" id="arCOG02957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208205_2_1_2"/>
    <dbReference type="OrthoDB" id="43651at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002318">
        <property type="entry name" value="PRK01253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE93671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="58" mass="6429" checksum="29C030CD07D96F94" modified="2011-06-28" version="1">MPSTKKKKENIPLVSMAGLIRYYDEENEKVKIDPKVALIGSIVLIAIILILSKLVPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B843</accession>
    <name>F4B843_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776782.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B843"/>
    <dbReference type="GeneID" id="10601494"/>
    <dbReference type="KEGG" id="aho:Ahos_1995"/>
    <dbReference type="eggNOG" id="arCOG03722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_4_0_2"/>
    <dbReference type="OrthoDB" id="30877at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF1">
        <property type="entry name" value="PAREP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="155" mass="18122" checksum="B0FEF17F178597F7" modified="2011-06-28" version="1">MQVPKQIEDILKKEALQKGIDEEVLLLDKLTRDLDPEVKVNLYKEKSTELLEEAKKYLEKKDFIQASEKAWGACASIVKAYAEKRGLEHYRHRQLEEIMSKIISAERSKELLSGWSICLRLHSNFYEGFMTEEDVKISLEEVEKFVKDMIKLLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B4</accession>
    <name>F4B8B4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribonuclease P protein component 2</fullName>
            <shortName evidence="2">RNase P component 2</shortName>
            <ecNumber evidence="2">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Pop5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rnp2</name>
        <name evidence="4" type="ordered locus">Ahos_0827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775629.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B4"/>
    <dbReference type="STRING" id="933801.Ahos_0827"/>
    <dbReference type="GeneID" id="10600294"/>
    <dbReference type="KEGG" id="aho:Ahos_0827"/>
    <dbReference type="eggNOG" id="arCOG01365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801579_0_0_2"/>
    <dbReference type="OrthoDB" id="34695at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 2" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="143" mass="16257" checksum="AF1B9A67C3229EC7" modified="2011-06-28" version="1">MLAQITIDIVLVIWLSILTYLILAKNNLYIRKIKNKKDIKSKRYIIFYIISEDQKISPKNVEDAIRSAVKEFLGSMWLEISNPRVIIYLNDSNEGIISTNRAGYKAVIASLPLVKTVDGKKVLIVPKRTTGSLKKAKRLIGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B8</accession>
    <name>F4B8B8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exosome complex component Rrp41</fullName>
            <ecNumber evidence="8">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rrp41</name>
        <name evidence="11" type="ordered locus">Ahos_0831</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteropolymeric RNA-tails.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase PH family. Rrp41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775633.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B8"/>
    <dbReference type="STRING" id="933801.Ahos_0831"/>
    <dbReference type="GeneID" id="10600299"/>
    <dbReference type="GeneID" id="42780473"/>
    <dbReference type="KEGG" id="aho:Ahos_0831"/>
    <dbReference type="eggNOG" id="arCOG01575">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063514_0_0_2"/>
    <dbReference type="OrthoDB" id="24266at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11366">
        <property type="entry name" value="RNase_PH_archRRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00591">
        <property type="entry name" value="Exosome_Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011807">
        <property type="entry name" value="Rrp41"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02065">
        <property type="entry name" value="ECX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 8" id="KW-0271">Exosome</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="24"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="243" mass="27033" checksum="52AEBF0ACC2A67A4" modified="2011-06-28" version="1">MLQIQKPKLILDNGLRLDGRRPDELRPMKMEVGVLKNADGSAIVEVGNTKILAAVYGPREMHPRHLALPNRAVLRVRYHMTPFSTDERKNPAPSRREIELSKVIREALESQILVEQFPRTSIDVFMEVLQADAGTRLASLMAASLAVVDAGIPVRDLIAAVAVGKADGVVVLDLNEPEDMWGEADMPVAMMPSIGQINLLQLNGNMTPEEFRQGMELAMKGINIIYNLEKETLKNKYAELKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C4</accession>
    <name>F4B8C4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Elongation factor 2</fullName>
            <shortName evidence="10">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fusA</name>
        <name evidence="12" type="ordered locus">Ahos_0837</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C4"/>
    <dbReference type="STRING" id="933801.Ahos_0837"/>
    <dbReference type="KEGG" id="aho:Ahos_0837"/>
    <dbReference type="eggNOG" id="arCOG01559">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_11_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01514">
        <property type="entry name" value="Elongation_Factor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000010">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000684">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="5"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="80"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="10">
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="723" mass="80465" checksum="9841C99D1853CF0F" modified="2011-06-28" version="1">MKDIERVRNIGIIAHVDHGKTTTSDTLLAAAGIISPKVAGEALALDYLSVEQQRGITVKAANVSLYHEVDGKGYVINLIDTPGHVDFSGRVTRSLRVLDGSIVVVDAVEGVMTQTETVLRQSLEERVRPILFINKIDRLVKELKLGPQEMMQKLLDIIKSVNDLIDMYAEPEFKDKWMVNPTQGTVVFGSAKDKWGFTLPIAQKKGINMKNVIDAYTSSDKNKINELASQVPIHEALLDAVIKFVPNPRDAQKYRIPKIWKGDMDNDLVKSMLNADPNGPIVMMINDMKVDPHAGLVATGRVFSGTLRSGEEVWLVNAKSPQKILQVSIYMGQLRELADEIPAGNIAAVLGLDKARAGETLVDINFKNLQGSFESLHYVSEPVVTIAVEPKNPKDLTKMIDALRKLTIEDPNLVVKINEETGEYLLSGMGFLHLEVSLQLLKDNYGIEVNTSPPIVVYRESIRAKSQVFEGKSPNKHNKFYISVEPLNEQTIELITNGTIREDMDSKEMAKILRDQADWDYDEAKRIIAVDENVNLFVDMTSGIQHLREIMDTLLQGFRLAMREGPLAHEPIRGLKVVLHDAVVHEDPAHRGPAQIYPAVRNAIFAGFLTSRPTLLEPLQKLDIRVPMDLVGNVTAVLTRKRGKILNMTQVGNVARILAEIPVADSYELASDLRGATGGRAFWGTEFSRWAPVPDSLLMDVIMKIRERKGLPKQIPKPEDFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G6</accession>
    <name>F4B8G6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775681.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G6"/>
    <dbReference type="STRING" id="933801.Ahos_0879"/>
    <dbReference type="GeneID" id="10600350"/>
    <dbReference type="KEGG" id="aho:Ahos_0879"/>
    <dbReference type="eggNOG" id="arCOG05934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167325_0_0_2"/>
    <dbReference type="OrthoDB" id="33957at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="94" mass="11161" checksum="EFFF25DD87B02CED" modified="2011-06-28" version="1">MSTRWLPKWKVVKINVKGKEVEVCYDDDLKLYACPFCNPICKKGGIPDYSTYFYHVEDLVSHIIAHKNALWLKKRPQEIREEEEEGEEEDNEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M4</accession>
    <name>F4B8M4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease V</fullName>
            <ecNumber evidence="8">3.1.21.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deoxyinosine 3'endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Deoxyribonuclease V</fullName>
            <shortName evidence="8">DNase V</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nfi</name>
        <name evidence="9" type="ordered locus">Ahos_2105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.</text>
            <dbReference type="EC" id="3.1.21.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.7" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776891.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M4"/>
    <dbReference type="STRING" id="933801.Ahos_2105"/>
    <dbReference type="GeneID" id="10601605"/>
    <dbReference type="KEGG" id="aho:Ahos_2105"/>
    <dbReference type="eggNOG" id="arCOG00929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047631_1_1_2"/>
    <dbReference type="OrthoDB" id="7885at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043737">
        <property type="term" value="F:deoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00801">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511:SF1">
        <property type="entry name" value="ENDONUCLEASE V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with target DNA" evidence="8">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="208" mass="23311" checksum="8A611BAD81E10037" modified="2011-06-28" version="1">MWGKEDYLLNFLILFQRLIAKNVKLTHLGLENVKTLCAVDVAYDREEGYAVAVKQEGDKIEYKITKGKVTFPYIPGFLFMREAPIMIKAIEGYQCDLLLVDGHGLAHPRKSGIATVIGVLLDIPTMGIAKSKLAGDIVEENGIYYVVINGEKVGVKAGKYFYSPGNKTDLQDVIEMSKRGYPEILKIADKLSKQAKKDKNMSSDYKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8P6</accession>
    <name>F4B8P6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxycytidine triphosphate deaminase (DcD-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8P6"/>
    <dbReference type="STRING" id="933801.Ahos_2127"/>
    <dbReference type="KEGG" id="aho:Ahos_2127"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="19819" checksum="8281D7A3D9D3D4B8" modified="2011-06-28" version="1">MSNMLLIFLTEVLFVILSHQSIKEIITKGVIRNYSEESIRENGYDLRICGEKYYEIEGNAELPNKKSEIKEIEFKDIAIFYPGKTYLFETCEELNMPNDLAALITLRSTLARNGFILPPTVIDAGYKGKITLALSSFYHNRIVKGTRTAHIIFLKLDKPTEKSYNGKYQGGVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S0</accession>
    <name>F4B8S0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ordered locus">Ahos_0904</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775706.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S0"/>
    <dbReference type="STRING" id="933801.Ahos_0904"/>
    <dbReference type="GeneID" id="10600376"/>
    <dbReference type="GeneID" id="42780553"/>
    <dbReference type="KEGG" id="aho:Ahos_0904"/>
    <dbReference type="eggNOG" id="arCOG02464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_0_2"/>
    <dbReference type="OrthoDB" id="9473at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="6">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="158" mass="16864" checksum="46AD3B8174023443" modified="2011-06-28" version="1">MPLVGVIMGSKSDWEYMKEAVDVLKSFGVSYEVKVVSAHRTPEFMVEYAKTARSRGIEVIIAGAGGAAHLPGMTASLTTLPVIGVPIPSKNLNGLDSLLSIVQMPYGVPVATVAIGGAKNAALLAIRILSIKYKDLEEKLNKFMEDMKNDVLSTSLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8S2</accession>
    <name>F4B8S2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inosine/uridine-preferring nucleoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8S2"/>
    <dbReference type="STRING" id="933801.Ahos_0906"/>
    <dbReference type="KEGG" id="aho:Ahos_0906"/>
    <dbReference type="eggNOG" id="arCOG04558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036838_11_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02649">
        <property type="entry name" value="nuc_hydro_CeIAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052775">
        <property type="entry name" value="IUN_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190:SF1">
        <property type="entry name" value="SI:CH211-201H21.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46190">
        <property type="entry name" value="SI:CH211-201H21.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="312" mass="35422" checksum="45153FEB06E3C303" modified="2011-06-28" version="1">MLRMTRHFIIDCDTAEDDIMSLFMLLRYGISVEGITIVEGNISFDQEVNNALWALEFIGKEIPVYPGSQRPLVKNYRTVENVHGKGGIGDKVVKPSKLKASNKHAVDAIIELADRYAGELEFLAISPLTNLALAYLKDKSLADKIKKVWIMGGTIYGRGNITPVAEYNIWVDPDAAKVIFSSGMDLTMVAWDLITNYTINDEEWDKIKSMNTKLSSFYIDIYAHYRNYAMTKQKMKGNPHPDLITTAIAIDSSVATKVEKQYVDIENCECLTRGMTVIDYLGIWNKEPNVNVVYEINKEKFISMLYNLLSWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8T8</accession>
    <name>F4B8T8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA (Guanine-N1)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0922</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775724.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8T8"/>
    <dbReference type="STRING" id="933801.Ahos_0922"/>
    <dbReference type="GeneID" id="10600394"/>
    <dbReference type="KEGG" id="aho:Ahos_0922"/>
    <dbReference type="eggNOG" id="arCOG00967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061952_0_0_2"/>
    <dbReference type="OrthoDB" id="14987at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053623">
        <property type="entry name" value="TRM10_methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016742">
        <property type="entry name" value="tRNA_m1G_mtfrase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041071">
        <property type="entry name" value="Trm10_mtase_Thprot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018978">
        <property type="entry name" value="tRNA_m1G_mtfrase_arc_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="4">
        <location>
            <begin position="79"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="292" mass="33685" checksum="3F244CBDBAB74D6D" modified="2011-06-28" version="1">MTLAKSLSYFLKKNGINEIYVNKVKRRGNSYLQDIALNILIKDFKLKETEILGKLKAEEDEIKIFSGKGSEKVNYEIAKNGKRDIIIDFPKFPVFIIDLSLWNRHSEEERNRLIVQILCSINSIRKYLWDYNLSINNFPQGFSLSILNKVRFNVAPEGNAIILNPYGEIEATEELIRNTQTFIIGGIIDRSGWKYATYEMAKIAKYDFPHVKIALRGSTVGVPDRINKIIEIILRVYYGEKLENVILDLQSNADKFNRLLVEKQKGNLKEAIQWLKPSEKVLRRLGIQSNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U6</accession>
    <name>F4B8U6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aconitate hydratase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0930</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775732.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U6"/>
    <dbReference type="STRING" id="933801.Ahos_0930"/>
    <dbReference type="GeneID" id="10600402"/>
    <dbReference type="KEGG" id="aho:Ahos_0930"/>
    <dbReference type="eggNOG" id="arCOG01697">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009520">
        <property type="entry name" value="PRK12881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="7">
        <location>
            <begin position="39"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="8">
        <location>
            <begin position="627"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="813" mass="90503" checksum="7F0B54900973BC61" modified="2011-06-28" version="1">MDLKNLPYSLRVLAINVSKHIDGEKITQDDLDAIINWKVGNEISFMPTRVIMQDYTGVPLLVDLAEMRSELARRGLNPEKVNPIIQSDLVIDHSIQVDYYGTSYSLTLNMKKEFERNEERYKFLKWAQRSFKNLRIIPPGKGIIHQINLEYLSKVINDNNEPEIVIGTDSHTTMIGGLGILGWGVGGLEAEAVMLGEPYHSIVPEVIGVKLEGEIREGVTPTDIVLYITELLRKKGVVGKYVEFFGSSLSKLTIQDRATIANMAPEYGATVGYFPIDEQTVKYLNLTGRDGDKVKKIAMELGIFYNEREEIKYSDVVEVNLSEIEPSMAGPKNPDERIKFGSLKIERKNKGNIIQDGDVVLAAITSCTNTSNPTVMLGAGILAKKAVELGLRSKPYVKTSLAPGSPVVVKYLKDAGLLPYLEALGFHVVGFGCTTCIGNAGPLIKEVENDIKTYGVEAYAVISGNRNFEGRINPLLKGTFLASPILVVAFAIAGRIDINYEEPIAFDPNGKPVYLRDIWPTLEEINSYMNLAMNPEYYISQYSTIFKGDENWEKLSVTPSPIYDWPKSTYIVEPPWFKEEDGKLEIKKARILLLLGDKITTDHISPAGPISPDSEAGKYLLSLGVKELNTFGARRGNHEVMIRGGFSNPKIKNYLVNKEGGYTIHFPDKREGTVYEVAMEYKKEGIPLVIVAGKQYGSGSSRDWAAKVTALLGIKAVLAESFERIHRSNLVAMGVLPIEIEDWRKLGIKGDELVSIEFTDLNPGSKVKIIFENNERKVETSGILRANTKTEIEYLKSGGILKYVLEKMIKDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U8</accession>
    <name>F4B8U8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2258 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775734.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U8"/>
    <dbReference type="STRING" id="933801.Ahos_0932"/>
    <dbReference type="GeneID" id="10600404"/>
    <dbReference type="KEGG" id="aho:Ahos_0932"/>
    <dbReference type="eggNOG" id="arCOG03772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691618_0_0_2"/>
    <dbReference type="OrthoDB" id="15362at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017140">
        <property type="entry name" value="ThermoDBP-RPs_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10015">
        <property type="entry name" value="ThermoDBP-RP_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17995" checksum="071A5E5EF5B5A034" modified="2011-06-28" version="1">MSEAQRDIERAEEYEETTPRTSVLGENKFELSTGLIIAARYADKLRRVALVSLSKMVPRDIIIRDVSEFNKNLYDKIVNQMKIDKLDVIKLVVSVRYDKSQNKLIFEDTKIIRYYTEEECKKQYESVIQENEKLKKEIDEIRKKLSDLLGSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Y7</accession>
    <name>F4B8Y7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved pro-fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776925.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Y7"/>
    <dbReference type="GeneID" id="10601641"/>
    <dbReference type="KEGG" id="aho:Ahos_2139"/>
    <dbReference type="eggNOG" id="arCOG09713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_390633_0_0_2"/>
    <dbReference type="OrthoDB" id="37211at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="706" mass="81176" checksum="9F9ED654CAED1916" modified="2011-06-28" version="1">MDRETLRRIISEKLKKNQNGDNNFVPQENPNNSVVPEKFSNQVNRDNPENVVNVVQSNFSNVQKFKCTTNTTNSGISTQSPVENIAEESKEIGTTNTTNSGISPNTSVEFRPEETEEIEDEDIEIKDHAIIIKTKKRYKFEIQYGFAGTIEDTTRIIGLKIPQLPQFELTTKKKDEVLSVLVTVDFYQMEGKQIYYIRNRKEVIEKLYTFRGDRHISKRLISDALDVWLDRVNPSKYIKWVDVREAVKDYQEWEEINQDPLGFFLSRSAEVIGNEKLKIAVLLSVVSSQLKRLYGIYRIHLILTGSSGVGKSSTVKSILKMFYDNADGVEDFIVLKLTRMTKESLGRLNINTLDGKVLFVEQLDNIEGVDYVREAMSEDRITTLTTVKDPEGNLISKTIIIPGQPSFISTNVTSNIDHQILNRSIQLYLSPAEDESIKEKIIRSILMRKSMDEGDLMRLKLVTYVWLKTRPNDPVFTEAVGSNVTKLLKKFINFRNIYRATEITRNLIRAVSSMFGHEKVEESDVDFVMEYFKKDIILTTLELSERDLQILMWLRDHGFVEGEKEEETKDVTTSEIAQVIKMSTQETKKLLDSLYDKGLLWKAYDGKRFSWAINRYGVKVLAELEKEVEEQQQEGEEDEDIIIEDQLLAYISSKLMGKEKVTEEELRAILEKTAQATDIEVQDMLIKALEKKGIIKDEGNGVWKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B924</accession>
    <name>F4B924_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2176</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776962.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B924"/>
    <dbReference type="STRING" id="933801.Ahos_2176"/>
    <dbReference type="GeneID" id="10601679"/>
    <dbReference type="KEGG" id="aho:Ahos_2176"/>
    <dbReference type="eggNOG" id="arCOG01282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_2"/>
    <dbReference type="OrthoDB" id="25212at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF107">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="6">
        <location>
            <begin position="270"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE95047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="395" mass="42486" checksum="6CEC681D53BA9C55" modified="2011-06-28" version="1">MTEVYIASAVRTPIGKFGGVFKDISPVDLGAIAIKEALKRANVDPKRVDIVIMGNILRAAFGQDLARQAAVKAGIPYEIDGYSVDMVCSSGMMSITNAVELIKTGDADIVVAGGMESMSQAALAVRSHVRWGVKMLMGKQLEFVDTMLMDGLTDPFNMKLMGEEADMVAKAHNFTRQELDEVAYQSHKRAAEATEKGLFASEIVPVEVNGTLITKDEGIRADTSIEKLSKLKPAFSKDGFHTAGNSSQLSDGASALVLMSEKAVKEFGVDPLARVLGFSWAGIESWRFTEAPIFAVKKLLQKLNMDISKFDYFENNEAFAVNSVLANRYLGIPYDRLNVFGGAIALGHPIGASGARIVTTLINVLSKMKGERGIASICHGTGGSTAMAIELLRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B942</accession>
    <name>F4B942_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776980.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B942"/>
    <dbReference type="STRING" id="933801.Ahos_2194"/>
    <dbReference type="GeneID" id="10601697"/>
    <dbReference type="KEGG" id="aho:Ahos_2194"/>
    <dbReference type="eggNOG" id="arCOG04261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253581_0_0_2"/>
    <dbReference type="OrthoDB" id="33752at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="212" mass="24596" checksum="E57493579EFAE20C" modified="2011-06-28" version="1">MIGGVRDRTTEALLRFGDRCKLILKAAISIAESNDKKELGDFDYKTLVEKLQEAGLDKDPKMILRALERDYGIIETTYKSANQHWWRFINIEEVKEAIGDEIEDPEIQLIKIQANSLNLTELERKLRFMLNKPVLSEVDRALFKKIAFEELNYVMEVYRKASMYEETYDIAEKVKSILTLATRVSMKIGKNYAQNKANDLLDPQKEPQAFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B958</accession>
    <name>F4B958_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0965</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775767.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B958"/>
    <dbReference type="STRING" id="933801.Ahos_0965"/>
    <dbReference type="GeneID" id="58788750"/>
    <dbReference type="KEGG" id="aho:Ahos_0965"/>
    <dbReference type="eggNOG" id="arCOG08476">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_212441_0_0_2"/>
    <dbReference type="OrthoDB" id="33285at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="52" mass="6107" checksum="AD568B65452088B5" modified="2011-06-28" version="1">MHSLDEALNLLEEVEKILKEYNPSKDDKELLGKLLSKNMRIEAEAYAKYIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B964</accession>
    <name>F4B964_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0971</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775773.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B964"/>
    <dbReference type="STRING" id="933801.Ahos_0971"/>
    <dbReference type="GeneID" id="10600443"/>
    <dbReference type="KEGG" id="aho:Ahos_0971"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_2"/>
    <dbReference type="OrthoDB" id="8297at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="293" mass="31800" checksum="FA8AF3562A05D525" modified="2011-06-28" version="1">MSSNPFRLQQPQRQPRDLRKVQPQIQGADLKIQMRMKNVKYKIAVLSGKGGVGKSFVSSNLSMALAAAGKKVGIVDVDFHGPSVPKMLGVRGQVLTADDEGIIPVNGPFGIKVVSIDFLLPKDDTPVVWRGAIKHTAIKQFLGDVKWGELDYLIIDMPPGTGDEALSVAQLVPNLTGFIIVTIPSEVSTLAVSKSVNFAKTVNAKILGVIENMSYFICPADNKPYYIFGEGKGKRMAEDMGVELLGQVPLDPKIAEANDLGEPFFLKYPDSPASKEFLSIADKVMKIVESNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B980</accession>
    <name>F4B980_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B980"/>
    <dbReference type="KEGG" id="aho:Ahos_0987"/>
    <dbReference type="HOGENOM" id="CLU_1987482_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="14783" checksum="7147E2D668CEA124" modified="2011-06-28" version="1">MRLIIAAMSRKRRLGIEGKNYHIFHYCFIFFIIFLFHFTFFKDISYCLVVCSYISLGKFGSVALQLPIRSCFTSTLFLQLLRGSLFPFSLLYNTWTVRLQYWGSTRTLYFHPLPIVVRGNRLGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B981</accession>
    <name>F4B981_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775790.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B981"/>
    <dbReference type="GeneID" id="71811288"/>
    <dbReference type="KEGG" id="aho:Ahos_0988"/>
    <dbReference type="eggNOG" id="arCOG07202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327113_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="98" mass="11056" checksum="0A9420CB81897DD2" modified="2011-06-28" version="1">MSNSVILHYPLILGITLFYSGGEIWRRKFQRSGKYRSILALVMIIGVAFLLYGIVISIHALTFSEYSLKLGVTSLISTILGFVLAYIHVIKRHKLAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A5</accession>
    <name>F4B9A5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775814.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A5"/>
    <dbReference type="STRING" id="933801.Ahos_1012"/>
    <dbReference type="GeneID" id="10600486"/>
    <dbReference type="KEGG" id="aho:Ahos_1012"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521785_0_0_2"/>
    <dbReference type="OrthoDB" id="43665at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="164" mass="18643" checksum="C5EB26D7124AA329" modified="2011-06-28" version="1">MQKLYTGRYVNLQALAQEINSILLQEGWESTVQPMMIGNPQYQDYMIRGLKKGHFHHAEELIVRVTGSPSEFRIIIEEEHIGPLGRELINRRLLKQIEKEINDGLFDLPMQQPTMQPINQQITPTVPSQQPIAPQAPQQIRCPQCGYLNPPGAKFCINCGARLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A9</accession>
    <name>F4B9A9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S-layer glycosylated protein, SlaA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775818.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9A9"/>
    <dbReference type="STRING" id="933801.Ahos_1016"/>
    <dbReference type="GeneID" id="10600490"/>
    <dbReference type="KEGG" id="aho:Ahos_1016"/>
    <dbReference type="eggNOG" id="arCOG06039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_294864_0_0_2"/>
    <dbReference type="OrthoDB" id="41909at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1027" mass="113790" checksum="B19AB0057ECD92B9" modified="2011-06-28" version="1">MEYDNTFNRSSMDLSTKKVISAGLVFIYALSLAMLVPMFLASNQGVITAVINPATGGNCLPGRYSGIVQASGVTLTAVNLTYVPPFNTTLPLKVCTQSIFNGTLYYYNSTGFNELVKKYGISMTPVDQVVNVTSKFFARTTEPVFLNKLPPYNSGNCYLFNTTPPTPFYYSYRTLAMVNSYFPNTQYINTSSEISDLYFNASYLPYNTKLTLSIYATPALPGIEGNIIFNFSVKLCANSTTYPDTEYTLHASFYFNNSFICELENIKTSVPGVASATSPTTIQFTSTAANYILLVDLGLWSNVSTVYVTGVEGTTYPSDTVATTINSGSFHPVIIPPAICIQYKTFYSNQINPELPNTSGPYSIPYMIANVTIYAPDDMLNWTYAQKYVGCVAVEYANPMWNPYCKINEAWCNNYGHGGNGLTVTLIVGNNKVVSTNFPYLALCHVSYTDGFHNFVYKFSIEFLLSCVDNITYMPITVKPNSTGLGGDMLYVLIKPCQLKDATILLTYNDSDYAVQHYFGPVDREIAIVCNNIKVYTPLLNMPKCVSLSTPFINGQVIDYDYGYNYALAGYVNTYYYNTTSHQYYKVGQYVYIQHVSGDSELLLGVCPGYKCASLSIYYPNVPPYYPASHEAKVGKIMGIYIEFANGTMERIYLSPSNITALLTTNVMKQMAPMPPFWNYSFEISITGLESILHITPNQALTVLNNSYIIVCYYDMASNSIVHNMTKLVSTPVTVAISPPSQAFYYAPATPFNDIVHPGCQIFYFVNVNATYPITVTLTDKSLGELSPTSIVTTTVTCIYVILYNGTILHYNSTVFPITLTETAPSSGVFTATLYVEVTNSKGTPVTAYPLNYSYIAIYNPATKMQIIVGKLSNIMPLEPSKYFKVGVVVDGLSANSTFTLTEIPNVIYNVTPIIYNYIDGELSVTVAANIPGYYYSGYLVLTIYNCTAKPCHPIYTYQIYFNFSTTSPYFVAAYDLLFLEPILNGHTYYITITAIVVPLTYEPFTTIGFVGKVFSGEYYFSAPAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B0</accession>
    <name>F4B9B0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-layer glycosylated protein, SlaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775819.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9B0"/>
    <dbReference type="STRING" id="933801.Ahos_1017"/>
    <dbReference type="GeneID" id="10600491"/>
    <dbReference type="KEGG" id="aho:Ahos_1017"/>
    <dbReference type="eggNOG" id="arCOG07272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525436_0_0_2"/>
    <dbReference type="OrthoDB" id="43990at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58100">
        <property type="entry name" value="Bacterial hemolysins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49478">
        <property type="entry name" value="Cna protein B-type domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="457"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="518" mass="55811" checksum="061EE9A4874F3205" modified="2011-06-28" version="1">MVKYLNLIVLGMLMFGIFVTLSLGSSTALTVYSPFPGEHVALICSKELILNISVYDPVAPEDSTVAVTISGPYDIKMTSAGVPIGQLNASHMLTVTDYEFPVTLFEANGTQISAFIPGQYNVTISVAGCSETIPIYVISPNEIEFLVYVYSNGLPLSGVTVSIYNVTNGGLLLTNTTNSQGLAELTVPYVYTMTNEYNITAVKPGYQLVYDVVTVPADHITPVTVKLTTKPITFVLTPVYFQDMGVTEPAEPAQLPGTPELVYIASAYMGTTFSIIINATESGMPVQGATISASYMINGKQMSSTATYIGNGQYNLSIILPNSTVPYDLELVVTGSYQTNTYTFVTLLSVQPNVVGEIQYLQSEVNNLETEVTTLESEILSLNETIMQQSSEIASLKMYLEGNVSYFSMQISNLESEIKYLNSTLMTLSSELTTVNSTLSNEVNSLTAEVHSLSTQVSTLSTELNNLNTTVNNLNANINNDNSKISSLTTLVYGGIILGVIALIIAIVAVVLVFRKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C5</accession>
    <name>F4B9C5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Peptidase M48 Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_1032</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775834.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C5"/>
    <dbReference type="STRING" id="933801.Ahos_1032"/>
    <dbReference type="GeneID" id="10600507"/>
    <dbReference type="KEGG" id="aho:Ahos_1032"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091224_0_0_2"/>
    <dbReference type="OrthoDB" id="28389at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07329">
        <property type="entry name" value="M56_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
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    </dbReference>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01435"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="30752" checksum="20A07B29716A700B" modified="2011-06-28" version="1">MVTRYWLRYYIISFASIAVIDVIVSSIVVSIPFFYIFQIIMIFGLWYFISPFTMIALLKMRKGGEGLVEVVDKVSKKFGVKTPRVYISYVDFPNALAFGNIFFRGMAVTSPLLDLLNDQELEAVIAHEISHLKNHDPEVLILSIIGINSIYSFLIYFFSCYLSIIVIFYFLGLFPLFFAIHRRVEKRADITAVKKDRSYAIPLETALIKIAYLSDKLPSYVLKNFPELQILLMKYDIISSNYGNGGLFRTHPSLSERLRYLSNYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F5</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F5"/>
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    <dbReference type="GeneID" id="10601735"/>
    <dbReference type="KEGG" id="aho:Ahos_2230"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077866_0_0_2"/>
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    </dbReference>
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        <property type="term" value="F:carbohydrate derivative binding"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="270" mass="30587" checksum="44C140416C86FBD5" modified="2011-06-28" version="1">MAIIEDIEEEISQNYKINSGVKLDEAYVTGAGDSFAASLVIEGKTNGRFRAVDPYDALSMNIDKPLVIVSISGKPKSNINLARKFKGKTKIYAITANPESELAKLADEVIYLPYRPKRILPGTLSFMMSLSALYDIANVEEDKGYGKEITIEDRAFFVGKGENYGIAYFAYLKLAEIFGWSSNFERLEQFFHSPIFSSRSRQVIIFSSGDEREKEARKLMNVNLTECGGAFCNLRTFLKSLIFTMRIKNWNKIYFLEDKEILNISSAMIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G9</accession>
    <name>F4B9G9_ACIHW</name>
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            <fullName evidence="3">Major facilitator superfamily MFS_1</fullName>
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    </protein>
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        <name evidence="3" type="ordered locus">Ahos_2244</name>
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    <organism evidence="3 4">
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_2244"/>
    <dbReference type="KEGG" id="aho:Ahos_2244"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0022857">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="117"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="147"/>
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    </feature>
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        <location>
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            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="248"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="349"/>
            <end position="367"/>
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        <location>
            <begin position="168"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="395" mass="43726" checksum="34347C21DB077BFB" modified="2011-06-28" version="1">MFIQMLTVIVSMTFAIRASNNMIATTLPLFSKYYFHFTQSEVGILSALLSLGTFITSGVINSRLQSNTRRRFFIISSVAYAIILPLFYFTNSLTIWIVSLLAGLSLGSIMPNIITYAGLLDDRKARERLLSIYTLALSASLVAGPAIETAVLTHFTIPYVFLFFEPFAILSAILSFFIKFPEENSKSARVSVFNNPGFKTAVINILAYNIPFSVILTFGGIYAVDYLHVSFAEVTALFSLFFTTSLLARIYLSIRPPQSVRLHAIAAISMTTIGLLAILFSNNLIIFSIALLILGFPHGLTYPLSVISISRTFKPYERNAANSIFFAIMMIIGIITPTISGFVAELIGIKYLFGTLIPVILILLILLNRYVGYVDKVVLEETRKNSLSISKDSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9H7</accession>
    <name>F4B9H7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777038.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9H7"/>
    <dbReference type="STRING" id="933801.Ahos_2252"/>
    <dbReference type="GeneID" id="10601758"/>
    <dbReference type="KEGG" id="aho:Ahos_2252"/>
    <dbReference type="eggNOG" id="arCOG05975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153618_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="138" mass="15438" checksum="705DE3DC54E4A547" modified="2011-06-28" version="1">MRVQVLIKEMNKDIIMKNLEFRIPLIGSAISLFIGGLLLIGKVPSILTLGTMIIVVILVSLSFLITRYKNLIHVGGILGILAIISSATAPAHNEALLNFGKSLYITTLDLLMILGFYVFPIIYIYFWVFTIIRRKTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R9</accession>
    <name>F4B9R9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775901.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R9"/>
    <dbReference type="GeneID" id="10600575"/>
    <dbReference type="KEGG" id="aho:Ahos_1101"/>
    <dbReference type="eggNOG" id="arCOG11954">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2893009_0_0_2"/>
    <dbReference type="OrthoDB" id="32873at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="62" mass="7147" checksum="125BB816E10C358D" modified="2011-06-28" version="1">MSDCYIKDGDKTCVIICGKLICDKDTVNDYGRLCEKCKRGDRKACIEMLERYGCWSASGWWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T1</accession>
    <name>F4B9T1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="8">ATase</shortName>
            <ecNumber evidence="3 8">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_1113</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775913.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T1"/>
    <dbReference type="STRING" id="933801.Ahos_1113"/>
    <dbReference type="GeneID" id="10600587"/>
    <dbReference type="KEGG" id="aho:Ahos_1113"/>
    <dbReference type="eggNOG" id="arCOG00094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="OrthoDB" id="5976at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="401" mass="43966" checksum="BA6D25F4B26C48CA" modified="2011-06-28" version="1">MAGIVGILAFDKIWNISKFMKYSLMGLQHRGYSSSGYAILNDKISSGHFSSPPEDIEINEHGWAGIGYTGSKEGYPIVFDDVALVIDGVIKEDPKIIAEKVLSSPEDLKDVRGAFSLIALTSEGKIIAYRDESGLKPLAIGGFGFDMAIIASEMTAMSVIGGEFRREINPGEMIILDKYNIESKKLKEPKKSYCSIEYIYQARIDSFVNEKSIYELRIRIGEELAREKPIDADTVIGVPDTAIPFAIGYSRVTGIPLDLGFTRTGSPIRTMLASNDFMKIVGVQLKLNVIKSAVFGKRVILIDDSMVTGTTLKNTIFSLRKMGAKEVHVLIGSPKLISYCPYGVEVPQSSELIAANLSEEEIAKVLGADSIYWLSIDGLYKVIGSKQLCLGCMLNSYPKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V2</accession>
    <name>F4B9V2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Paired CBS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2297</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777083.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V2"/>
    <dbReference type="STRING" id="933801.Ahos_2297"/>
    <dbReference type="GeneID" id="10601805"/>
    <dbReference type="KEGG" id="aho:Ahos_2297"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076812_3_0_2"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="71"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="135"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="200"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="29960" checksum="0C33B20BA4ECE0B6" modified="2011-06-28" version="1">MGIIRSSIILRPYDTLLYATKIMIMEYVPKAIVTDEKGFPLGALTQKDIIKFVYEKGEEISFDKVYVSEVMRKDIVCVNESIDPLEAAEIMIDKKQPLLVVCSDDGKALGMIIKSDLTQYYASQIRGLQKTEEYMTSPAITISKSDSLINAVKTLVEKDVSRLIVVNEDKIEGQLTTTDLLYMTPAIKYKECKINVSEVMSPNIIVVDAGEDLASAAKLMASRKIKGIPVVKGDKLSGVITTTDVTRALLDDRVRKYLYEIKMYTSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9X4</accession>
    <name>F4B9X4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferredoxin--NADP reductase</fullName>
            <shortName evidence="5">FNR</shortName>
            <shortName evidence="5">Fd-NADP(+) reductase</shortName>
            <ecNumber evidence="5">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 reduced [2Fe-2S]-[ferredoxin] + NADP(+) + H(+) = 2 oxidized [2Fe-2S]-[ferredoxin] + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ferredoxin--NADP reductase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777105.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9X4"/>
    <dbReference type="STRING" id="933801.Ahos_2319"/>
    <dbReference type="GeneID" id="10601827"/>
    <dbReference type="KEGG" id="aho:Ahos_2319"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_5_2"/>
    <dbReference type="OrthoDB" id="27340at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01685">
        <property type="entry name" value="FENR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022890">
        <property type="entry name" value="Fd--NADP_Rdtase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0274">FAD</keyword>
    <keyword evidence="1 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="7">
        <location>
            <begin position="4"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="333" mass="36847" checksum="2D83028026640D92" modified="2011-06-28" version="1">MSEYDMIIIGGGPVGLFGAFYATLRDMKTMIIDSQDELGGQLVSLYPEKMVYDVGGYPGILGYELAQKLVEQAKMFSPDIRLKEWVDWIQKTDDNMWLVKTDKGNQFKTKTVLIAMGIGKIMPSRLGAKGELEYENKGVYYTVRRKKDFEGKRVLIVGGGDAAVDWALTLAPVAKSVTLIHRRDQFRAHERSVKELFQVATVYTWHELKEVKGDGNKVTQAIIFDNRTKEEKTLDVDAVIISIGFKGDLGNLPKWGVAMKGRDVIVNARMETNLPGVYAAGDIALQEGAPKLALIAVGFGQVAIATSVAKKYIDPNASLFAGHSSEMDKFKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y0</accession>
    <name>F4B9Y0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminotransferase class V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2325</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777111.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y0"/>
    <dbReference type="STRING" id="933801.Ahos_2325"/>
    <dbReference type="GeneID" id="10601833"/>
    <dbReference type="KEGG" id="aho:Ahos_2325"/>
    <dbReference type="eggNOG" id="arCOG00065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_1_2"/>
    <dbReference type="OrthoDB" id="5817at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF8">
        <property type="entry name" value="CYSTEINE DESULFURASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="17"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="368" mass="41567" checksum="043EC929D391FB71" modified="2011-06-28" version="1">MFWEDFRELVPATKNYIYLNHAALSPTPLPILYETFRYLYEVSKSGTIADNEEQKDELFHIRKNISNLIKADPLEISLIPNTSFGINEVAHGIEYDDKSNVVTDSLEFPATVYPFLKIKKLEKRIVNASPYDIEDKIIENIDENTKIVSISHVSFNTGVRIDVQKIVTKAKKVGALVLLDIIQSAGAIKINVKDLGIDFAVAGGYKWLMSPQGSGFMYIRKGLLEDPPFYGWKSAKNYLDFDPTKFELEKGPRRIEIGTIDIAANLGLSKSAEIISSHEDEIEKRVLQLSAYAIDLAEDKKFEVITPKEKRAGIVVIKVKKPKEITSRLAQRNIIVSPRGEGIRISTHFYNTEEEIEKTINSISQEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z6</accession>
    <name>F4B9Z6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8 10">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nadE</name>
        <name evidence="12" type="ordered locus">Ahos_2341</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="8 10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777127.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z6"/>
    <dbReference type="STRING" id="933801.Ahos_2341"/>
    <dbReference type="GeneID" id="10601849"/>
    <dbReference type="KEGG" id="aho:Ahos_2341"/>
    <dbReference type="eggNOG" id="arCOG00069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000106">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010587">
        <property type="entry name" value="PRK13980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD/GMP synthase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE95212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="280" mass="31701" checksum="ACEC03AB409AD213" modified="2011-06-28" version="1">MRVQSVLQELKDVNYSVISEYIIRRISEYITESGKNGGIIGLSGGIDSSVTTVLLSKATQNYFILLMPSSSTPQKDMEDAKKIINMIGANNKYKIINIDPILESFKNEIKTNDKLVLGNIKARIRMILLYAYAQIMNYLVIGTGDKSELLLGYFTKYGDGGVDILPIGDLYKTQVRELGRYLGLPEDIVTKPSSPALWEGQTAEGELGVSYDIIDAILYLRVEKMMNIESIAKDLQIDTEIVHKIDRMIKTSQHKRLPPEIFRLSGRAINSDWRYPREWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA14</accession>
    <name>F4BA14_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="2">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">OMP decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1127</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA14"/>
    <dbReference type="STRING" id="933801.Ahos_1127"/>
    <dbReference type="KEGG" id="aho:Ahos_1127"/>
    <dbReference type="eggNOG" id="arCOG00081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_2_0_2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="8">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="23576" checksum="23305095E21E561E" modified="2011-06-28" version="1">MDEEIPQHVLLKISEYFYGIKIGYPLLLRKGIEGVKEILRKLEVDEIIMDLKLADIDNTMINIVKQLDFANSFIAHSFIGIDGALGELKNFLDNEGKKLYLLASMSHKGWNDEFYPYIKTIIKSLNPYGIVAPATKPNVLRIVRDDFPDKILISPGVGAQGGKIGDALCNGADYEIIGRTIYTSSDPENKVKEIIKIQEEKINECKKTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA44</accession>
    <name>F4BA44_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="9">AdoMetDC</shortName>
            <shortName evidence="9">SAMDC</shortName>
            <ecNumber evidence="9">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="9">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="9">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="9" type="primary">speH</name>
        <name evidence="10" type="ordered locus">Ahos_1157</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="9">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA44"/>
    <dbReference type="STRING" id="933801.Ahos_1157"/>
    <dbReference type="KEGG" id="aho:Ahos_1157"/>
    <dbReference type="eggNOG" id="arCOG00279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_1_2"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000005">
        <property type="entry name" value="Arginine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866:SF2">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="1 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 9" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 9" id="KW-0704">Schiff base</keyword>
    <keyword evidence="9" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023344060" description="S-adenosylmethionine decarboxylase beta chain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023344061" description="S-adenosylmethionine decarboxylase alpha chain" evidence="9">
        <location>
            <begin position="69"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="9">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="9">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="9">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="9">
        <location>
            <begin position="68"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="9">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="13545" checksum="407496EF01747A63" modified="2011-06-28" version="1">MGINQEVPKVIGKQVYGSLYDCDEEVLKDVEKLKNIVINAAKIGNMTLLDVKAWKIGEGASVVAIVLESHITIHTWPEYKFATVDVYSCGAKSDPKKAFAYIVKELGAKRYSMNEADRSSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3M9</accession>
    <name>F4B3M9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved proviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774835.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3M9"/>
    <dbReference type="STRING" id="933801.Ahos_0023"/>
    <dbReference type="GeneID" id="10599463"/>
    <dbReference type="KEGG" id="aho:Ahos_0023"/>
    <dbReference type="eggNOG" id="arCOG00996">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873238_0_0_2"/>
    <dbReference type="OrthoDB" id="6871at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="295" mass="33867" checksum="E7FF35D37E15D7EE" modified="2011-06-28" version="1">MKVLLGIPDERIPIWELGTPLMINQLVWDSIPWDNETWVDSGGYQIMVKGFSLQVDAVLKKYLSLHADTFMSLDVPSFCTDVNEINFKNFEYLYEKINVMPIIHAYTPDGIEKAVEFYSQYTDKIAYGGIVPPSLNGSKKIVIMIYHYLRRKVKYLHVLGAGSPYMRELFFNADSVDTATYRIKASRGLVIIPGKGERYVGDRKIVWKAKRATDEDIALLLNFLDKTRFPYKVDLKNWISRALINAWVLLNSKYEAHNYLIDFSRKVDKMGSDYLKGEIEEMCNAVPTSFGSANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N5</accession>
    <name>F4B3N5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fmu (Sun) domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0029</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N5"/>
    <dbReference type="STRING" id="933801.Ahos_0029"/>
    <dbReference type="KEGG" id="aho:Ahos_0029"/>
    <dbReference type="eggNOG" id="arCOG00975">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_7_6_2"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF70">
        <property type="entry name" value="TRNA_RRNA CYTOSINE-C5-METHYLASE, NOL1_NOP2_SUN FAMILY, FUSED TO N-TERMINAL NUSB REGULATOR DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="5">
        <location>
            <begin position="79"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE92924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="350" mass="40525" checksum="CBD68EDF94D2B247" modified="2011-06-28" version="1">MKKNLSPERAFDIAFKKYMQGNRKELYREFIEYIKKYLYERNVYPGISPREVDMTINPDPMLSFPKWIFERLYALLGEEGIKGIYNHKTWARVNELKANVNDVIRLLESEGYKVKRTEINFLLEIESADKRISDSTAFKEGLLIPQDKSSVLAVMTLDPKPYEKILEIGSAPGVKSSLIQQLTRNKSFLISIDISEKRIMQQKKLMEKWGVSNVELIVADALHLPIRKADKVFIDAPCSNSGTINVDPSVVLRLNKRKLHELSSIQIGILREASKLKTQVVYITCSLFPEEGEKVVEKFGRNLVRIPNEGHEGYKKSRVWLRVFRTYPHKDFSEGFFIAKLDFSSPHFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P7</accession>
    <name>F4B3P7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heavy-metal chelation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776000.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P7"/>
    <dbReference type="STRING" id="933801.Ahos_1201"/>
    <dbReference type="GeneID" id="10600677"/>
    <dbReference type="KEGG" id="aho:Ahos_1201"/>
    <dbReference type="eggNOG" id="arCOG03216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217596_0_0_2"/>
    <dbReference type="OrthoDB" id="36516at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159713">
        <property type="entry name" value="Dhaf3308-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="7"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Putative heavy-metal chelation" evidence="1">
        <location>
            <begin position="95"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="24668" checksum="9EB718E3EE86C619" modified="2011-06-28" version="1">MILDEIIDELSYDLKQRKIINICVGTSYTAVILDDQSMGVSHTIAEGEVDYAGEIIGKNAYDVAVDVDNPLKRSISVAILNSISTGKLTSGDPLTLYSGGKVCAFGYYPYISAGNFSSVVLYDFSTQPQNNAKPFSQFNGETCDVAVIFGSALINNTIDKIIKNVKADHLILTGISSVEAISTLKKYGFEAIGKIVPVDQYRAFRTICEGGSAKQLSKYVTKMYLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3R5</accession>
    <name>F4B3R5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoacid oxidoreductase (ferredoxin)</fullName>
            <ecNumber evidence="5">1.2.7.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1219</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the coenzyme A-dependent oxidative decarboxylation of different 2-oxoacids such as 2-oxoglutarate, pyruvate and 2-oxobutyrate to form their CoA derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 2-oxocarboxylate + 2 oxidized [2Fe-2S]-[ferredoxin] + CoA = an acyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="1.2.7.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.11" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3R5"/>
    <dbReference type="STRING" id="933801.Ahos_1219"/>
    <dbReference type="KEGG" id="aho:Ahos_1219"/>
    <dbReference type="eggNOG" id="arCOG01053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_4_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018491">
        <property type="term" value="F:2-oxobutyrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514:SF1">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase N-terminal" evidence="8">
        <location>
            <begin position="58"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="293" mass="32423" checksum="E4E9311E4051411A" modified="2011-06-28" version="1">MHNNENLLNKNLGINRGLDVTPMGERDGIPISIDELNKLKAIAERARRNVIKMQFYDHSIHVGSSLSSIEILTTLIFRYIRDGQEKINKDWLILSKGHAAPALYAVLHEKGLIKEEELWKIQAIDGLLQGHPEIYIPGVDMSTGSLGQGLSFGIGVATGIKMAKGNGRVFVIMGDGEQDEGEVWEAATHAVARNLDNLIAFIEVNGYQLDAKTSEVKPKDFLPDVYKAIGWRTFSCDGHDILSIINAVEEALKARKPAVIFAKTLRGKGFTPIENTKIQRANPDVARQFLLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3T0</accession>
    <name>F4B3T0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="AEE94117.1"/>
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    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Hydratase-Decarb/MhpD_sf"/>
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        <property type="entry name" value="2-KETO-4-PENTENOATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30143">
        <property type="entry name" value="ACID HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="AEE94138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25703" checksum="CBD85044FCB8E1A4" modified="2011-06-28" version="1">MDKADLLFNAYKSRKEIEPFEVNEKEAEEIFKKFSSRLVEEEGLGGYKISLVTREHLKRFHGKEPMYGILTKPMITSDKEIELWFNHNFAEVEVVFFADSCRNDNFPQCIKETRLGVELPATRFNTWNLNALQLKADDSAAGRLYIGEQVSLPIKGVEMLINDKLVGRGVPNFIYGGPMDMVRWLISKIGEVNGYVSSGVFIGPFEVKKGDKITILGDVNFEIKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W8</accession>
    <name>F4B3W8_ACIHW</name>
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    </protein>
    <gene>
        <name evidence="21" type="ordered locus">Ahos_1272</name>
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    <organism evidence="21 22">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 18">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15 16">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 18"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776071.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W8"/>
    <dbReference type="STRING" id="933801.Ahos_1272"/>
    <dbReference type="GeneID" id="10600753"/>
    <dbReference type="KEGG" id="aho:Ahos_1272"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_2_2"/>
    <dbReference type="OrthoDB" id="36124at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006576">
        <property type="term" value="P:biogenic amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Probable glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
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        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407:SF1">
        <property type="entry name" value="LENGSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="19">
        <location>
            <begin position="15"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="20">
        <location>
            <begin position="107"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="261"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="268"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="14">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AEE94155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="471" mass="53289" checksum="DCC0CCD1E025E47C" modified="2011-06-28" version="1">MPSSPEDMLKFLKDNKIEWVDLQFTDVPGRLHHITIPASEFDENSVKTGFGKLDGSSIKGFTMIYESDMVLLPVPETMTLIPWTPGVARVITKVFWGGGKGRFERDPRFIAEEAEKYQLSQGYTSFFGPEMEFFIFDKIDLDVATPQRGTGYKIIAREAPWQNNGGFVIRYKEGYYPAPPVDQLMDVRLEAVETLVKYFGFTIEATHHEVATGGQGEIDFRFSTLADTADKVQTLKYVVKNVAAKHGMVATFMPKPMFGDNGTGMHTHFSLWTADGKKNLMYDPNDEYAEISQTGRYVIGGILHHARALSAIVSASVNSYRRLVPGFEAPVYIAWSKSNRSAIIRVPSYYKGIEKAKRIEYRAPDPSCNPYLAFAAILMAALDGVNKKIDPGDPVDENIYHLTPEKRKQLGIKELPRSLDEALDELESDKEFLKPVFNSTILDTYIDLKREEAKTLQMYPHPMELYYYLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Z3</accession>
    <name>F4B3Z3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0062</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774874.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Z3"/>
    <dbReference type="STRING" id="933801.Ahos_0062"/>
    <dbReference type="GeneID" id="10599503"/>
    <dbReference type="KEGG" id="aho:Ahos_0062"/>
    <dbReference type="eggNOG" id="arCOG00555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000809_3_2_2"/>
    <dbReference type="OrthoDB" id="36796at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="64"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="264"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE92957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="740" mass="82949" checksum="75392CE0E3303FC6" modified="2011-06-28" version="1">MEVLDKLTEGLKLFSADVAHVHTETALEPELGSKVDDLQIDERLKNSLKNIGITRLYKFQEKALEEIESRKNVLIISGTGTGKTEAFLIPILDLALKGEKSVIVYPTKALARDQLSRILRLIKNLPINVGIIDGDTPKIERDRLYASPPDILITNPDMIHVGLPLSPNFRRIIRSAEHFVFDEIHVYEGVLGSHLRMIIDRLKEFTNDIHIIASSATIGASPYMFQELFDGPGEIIEGTKRRRGMAFHILVNSKGMSRWTLAAYLAALLIKNGLRTLVFTDSQQMAEVTAKIADRFGVNMQVHRAGILPSERIKIEEDLKNGKIMGVVATPTLELGIDIGDLDVVIMAEDPPSYSKYIQRAGRAGRRNRIGYIITILGDDPIDSYFLRKPEEFFNRKLTPLPFDTTNLEVIKVHAAAYLLEKGMIKLDSLPPLWVKALKELDKEGKVKIVNDVAYATKQTKIFVINSSLRSTGPIIKIYNGEKKIGERELPVALYDIYPNAVYLVSKKNYIVKDVDLNTLVARVERVDKDLPYYTKPLYDVDMVNFTEIESRKVYGLPVKYGEIEVRISVHGYIIHDFYSKKENRGKEIEYDIPLTYTYKTKGLIIKHPILDDFSLIDGMEAYHATEHVLISAGRVVAGASLTDLSGISYPSGHVVIYDSVVGGSGVAKLLYDRLEDAYEVALDIVGKCDCEDGCPKCVYSPYCGNNNKMLSRKKSFRLISEVIKGKATSCDNMWGDSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B412</accession>
    <name>F4B412_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger SWIM domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774893.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B412"/>
    <dbReference type="STRING" id="933801.Ahos_0083"/>
    <dbReference type="GeneID" id="10599523"/>
    <dbReference type="KEGG" id="aho:Ahos_0083"/>
    <dbReference type="eggNOG" id="arCOG08479">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519556_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE92976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9505" checksum="BEA046DE6875CB9A" modified="2011-06-28" version="1">MYSIITYSVMTRILSIKDTPEGRIIEGLVPAKCIVGFHKVRIKVTNSKMIESVCSCGSTLCPHAVKLYLFYMAHVKRNENSVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B421</accession>
    <name>F4B421_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774902.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B421"/>
    <dbReference type="STRING" id="933801.Ahos_0092"/>
    <dbReference type="GeneID" id="10599532"/>
    <dbReference type="KEGG" id="aho:Ahos_0092"/>
    <dbReference type="eggNOG" id="arCOG07265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154341_0_0_2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="STK_08120-like"/>
    </dbReference>
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        <property type="entry name" value="STK_08120-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15570" checksum="39EE87F0F390ACE6" modified="2011-06-28" version="1">MRISVEIQTKHESNALLVVLSDPSFVLPNLLPPIKEVKISEGKYTCKGKFLGMPFEIVGNYYLSTEGRIKYVFTINKGGTGNLEFLIEQGKVTLIFDYDGWFEKISRLFISRWINDFKKNFEEKVRLKRIESKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B444</accession>
    <name>F4B444_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Transport protein, putative</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_0115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B444"/>
    <dbReference type="STRING" id="933801.Ahos_0115"/>
    <dbReference type="GeneID" id="10599555"/>
    <dbReference type="KEGG" id="aho:Ahos_0115"/>
    <dbReference type="eggNOG" id="arCOG05344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800770_0_0_2"/>
    <dbReference type="OrthoDB" id="37025at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="346" mass="36542" checksum="4AD893967AB7D381" modified="2011-06-28" version="1">MSISKTFSGVTSFIAYTLATYVMVAPAFTVKQFELPAWLAFLIVSIPFGGRVIGSLLYQRIVAILGSRLTFLSSMVVLGILSIGSSTLNVPILIPLRLLVGVAFGIATSLAVEQAVRSGNRLIIALTMSGWAFGWIGGALSYLALGEWQLIALSGAITLPFSMSYKRVSVFSVEVGKLGIPSISSILIFFFSFEPAFALQLAPAIVENQGGIVWLIIGYIVAIPMYIFVPVISSLLGETRTALLYTMTSAISGVTFFLTGSPYVLVVFTAFGLGINSIAPRLAASYGASARNMGIALNTAALGGVAVPVISSLDIKFLASLFTALSMVILLVMSVKKPNALLVRSA</sequence>
</entry>
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    <accession>F4B4B6</accession>
    <name>F4B4B6_ACIHW</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776139.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4B6"/>
    <dbReference type="STRING" id="933801.Ahos_1341"/>
    <dbReference type="GeneID" id="10600827"/>
    <dbReference type="KEGG" id="aho:Ahos_1341"/>
    <dbReference type="eggNOG" id="arCOG07219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559379_0_0_2"/>
    <dbReference type="OrthoDB" id="43038at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="19652" checksum="9015D23E1BFE7D80" modified="2011-06-28" version="1">MKATVEYYKPEIINANNFSLVSLKKYKEEEVDYSENLESKTINGEVIIVKYKEGEIINANVDCKYKFPFPVKIFRIGIPKEFNNENVKIDYINVAVDSFIIGLNFDLKDYPFIILDTSDGTKIISSSDINEVKPTIVKNKKKKKSKKAKKKKNAKKSSKKRKVKSKSARKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B8</accession>
    <name>F4B4B8_ACIHW</name>
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        <recommendedName>
            <fullName evidence="3">DUF190 domain-containing protein</fullName>
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    </protein>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776141.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4B8"/>
    <dbReference type="STRING" id="933801.Ahos_1343"/>
    <dbReference type="GeneID" id="10600829"/>
    <dbReference type="KEGG" id="aho:Ahos_1343"/>
    <dbReference type="eggNOG" id="arCOG07222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176007_0_0_2"/>
    <dbReference type="OrthoDB" id="36088at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11366" checksum="A672ACDBF8DD5518" modified="2011-06-28" version="1">MFSVKLLVLEDPGRLRDVFYSIEGILANICKPIRLGASYICSVSKNTLISAYLSGNLKNFQLIIEIESEDAEELTTTLDRIINELKSKGIHITLFNTSTTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D9</accession>
    <name>F4B4D9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774945.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D9"/>
    <dbReference type="STRING" id="933801.Ahos_0135"/>
    <dbReference type="GeneID" id="10599575"/>
    <dbReference type="KEGG" id="aho:Ahos_0135"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_3_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="4"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="392" mass="42602" checksum="9BC1AC830CC6F10B" modified="2011-06-28" version="1">MEANIRYIVLSRIFRSLPLGYMSFLVPLYLHRIGIPTFLIGIYFLIATISMSFLLVLSGFLGDAYGKRNILLLLSILFSLNLFIFAFIKIPLIVFLTSVLGVGTASGGGIGGGAGGGPFNPLQTALIADYSRPEERTKLYSINFAAATISAFAGGLISDLITTVYPPSISYSLLFTLGFIISLFSIFFVYLIDKDRGTGKISIPKASFSSISKISIAGGLGSIGLGMVMPLIPLWFKLNFNATESEISYLYSISYGLVGISFLFADRIEKIIGRVKSISTLRGLSSLLLLLIPFSPTFLIASILFLARGVTYTVTIPMRQSLAMDVFSDKERASGTSITGLARRIPYGLGSLISGALFSAGLFALSIFLGGVISLFDPLLYYIFFRNYEKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E1</accession>
    <name>F4B4E1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774947.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4E1"/>
    <dbReference type="STRING" id="933801.Ahos_0137"/>
    <dbReference type="GeneID" id="10599577"/>
    <dbReference type="KEGG" id="aho:Ahos_0137"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_2"/>
    <dbReference type="OrthoDB" id="10157at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005056">
        <property type="entry name" value="PRK06463.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760:SF133">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42760">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASES/REDUCTASES FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="251" mass="27915" checksum="0FFB504C6D5466DC" modified="2011-06-28" version="1">MVSGKTVVITGGTRGIGRAIAEKFFSAGNKVIVLYNSSDQEAEKMKERGFYVLKCDVSKRDQVIDTAKKVSEITKTVDILVNNAGIWYLIPFEEFDDEKYRRMLEINLNGAIYVTHAFLPLMKEKGGAIINIASNAGIGTSALGTTFYSITKAGIIILTRRLAYELGKYNIRVNAVAPGWVETDMTIGGKSEEEIEKLRQWFRERTMLHTTGKPEDIANIVYFLASDEARYITGQVIVADGGRIDYLTHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4E8</accession>
    <name>F4B4E8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thermopsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774954.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4E8"/>
    <dbReference type="STRING" id="933801.Ahos_0144"/>
    <dbReference type="GeneID" id="10599584"/>
    <dbReference type="KEGG" id="aho:Ahos_0144"/>
    <dbReference type="eggNOG" id="arCOG03670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_307901_0_0_2"/>
    <dbReference type="OrthoDB" id="36243at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007981">
        <property type="entry name" value="Peptidase_A5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05317">
        <property type="entry name" value="Thermopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1092"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1117" mass="125597" checksum="75061142F96C5829" modified="2011-06-28" version="1">MEVKLVKAFLIILLLLSPFNYFSNFGHSLTTNRFTHVYPPRIDPKLSHVQGGVNVYSLYNSEPAPMGIADYGIGPNGPYIRNTSQVLAKAHICSLNVDSSINTDCVSAQLNVVLSYCYNGNQYSIWLQDVALIMLFNNTIKFIDNIWNLSSVNANVIGVKGKGTLCTYHGVTFYYYCANGYPGSPCVFSYPLNFCMLINVSENSLGEPVAYFWYNDGHGWVNYDTVTFLFPHSRNVNITINGYQYTGSGDFYDIEFDIVGPGGGTCAYFNNACVYLYLYYWNGHNFQEIKNAYNFGSDTGETSNNVIASGYYIPSSGELTSGLKAGQGSLAQIWSSNQVSVVKVRTSIRSGYVSFSQSKWNSTLPMYKFCGYCAIITLYPGNYNVIILNNQGKISGESYVKLLGGKEIQTYCYHFKLISPQSICIFYGENNVEYIEICGMGYANLSLKLPNSISYVDNFTPFINGKEVVELILIPHVLYCSAEGILTVTLSSGLTCTSTFKIVATTRIIEVCIKVESIGNTLPEEPKIKVQFPNGSIEEITQGKYLLPAGSTYCLPKCIYENNQIRWILNSSNEGKFTLQGEYTFYYYEEYLVNFSFKVIGSSYNFTPEVYYYSAGKLNESLAGLIWADYNSTYYYQQCVNYGKVRYISFNYTGEISSFKAIAIYYQQYYVNVNSMAPLYAEINGVNQSFSSGWFNYSTRIIIENITFYPCAYERYIISSVTPSIAFTVDSPVNVKVSYIIQYYVEVNAKIPVYAFVNGSNVSFSSGWYNKGDHIKVENVTWYKCPFTRFIITSINPSNFTVGDHIIVNVSCLEQFYVHVYAKIPLYAEVNGINGSFTSGWYNDGICIYVENVTFYPCAYERYIISSIKPSSIRLCKPLNVTISCFIQFFICVKSKIPVYAFVNGSNVSFSSGWYNKGDHIKVENVPFYISPKERCVISSPLPSFTVENHTVVTLKTIIQFLVCVISVIPIKAYVNGEETTIGTSWINEGTKIEVINYTYYPCKDERYVITSINPSSTIFVNSSVKLAIYSIKQFLVCINGIKSWYNAGSKIVLNATIPIYEVGEFIGTYNVSPGSVIVVNSPVVERLVENLNYVFIILVAVIVLAISIIIFIKIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4F4</accession>
    <name>F4B4F4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Archaeal Type IV pilin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774960.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4F4"/>
    <dbReference type="STRING" id="933801.Ahos_0150"/>
    <dbReference type="GeneID" id="10599590"/>
    <dbReference type="KEGG" id="aho:Ahos_0150"/>
    <dbReference type="eggNOG" id="arCOG03871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1782570_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="13239" checksum="28EE726A5349D7EF" modified="2011-06-28" version="1">MIIQKAKKSKKGLSGAITALILVIASVIIALVVVFFAFGYLGAFSSTPTVTEAGYGTVSSSTLTVTLVSSGNVNIIGVEYGASSIAASASLTAGTNVVTITISGLGITPVPGQSYSFTLVLSDGGTVTVTATAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G0</accession>
    <name>F4B4G0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        </citation>
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            <strain>W1</strain>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="505" mass="55479" checksum="1C1038DD8969FCCB" modified="2011-06-28" version="1">MQFVRNSSGLVKNASLTDAMMLNIANMGAGLAIFTGISPYIVKGAVLWLASLITFLITLPLVFLYTFFIIEIHRTGGDYVWLSRKLNGKIGSIMGIALAFNMPPYFALSAFFSVAAINTVLEVIGTLNHEKALLNLANTVFVNPYCSSITLTQEILIYILAAIAFAIIIGINILKPKWGFSLVTALGTLAILGTIVSMIVVGINVSDFHTKISTFLADFNLTPSTYTGPTFSLPATIYMIPYFASFAYIWLYAGPAVSAEIKSERGIKYNIILGSLLTLFLITVPFYLLCIAGGYGFNFSLFPTATYNFWSVAIALAGNQALQWFIGISLISWEFFVMAFGVIVFARYIFAFSFDRLFPEIFSRLNRSSSPVYAHLLDFAVTLVFLAVPIISPEGVQALYSYTPLAIAYLFLVSLAGAKFSLEGKKRIGMLISSIISAAFMIFMGYEAFTNPYFGVISNGQPFWPGIAYVLSLIGLGIVIYVASKEYNLRKGINIDLNYKEIPPE</sequence>
</entry>
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    <accession>F4B4J5</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <dbReference type="PubMed" id="21607549"/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        </source>
    </evidence>
    <sequence length="498" mass="53848" checksum="BEEF79A9E107FFEF" modified="2011-06-28" version="1">MSKSFPSPFRPIDSLKLTFNHIKIWYTAGMGFFTDAYDLLIIGYILATIEDSYKYAGIVMPGFTSYLVGPDKDFWNGLLASIALWAAIVGQLIFGFLGDYWGRKKVYGVEATLLTIGAFLSALSPNLIWLIIFRFIMGVGIGGDYPISATIMSEYANVKDRGKLIALVFSNQALGSLAAAAVGIISAVVLPPCIAWRVMAGIGAFPAATVIYLRRKVPETPRYSLLANGNVEEAKKAASFLGGDIETRQPVLAKRMSLSEILSKYGTLLIGTAIPWFILDIAFYGTGLYSSPIIAPIFGSPFPKSATVLPLSEFQGDLAYALFLGAVPYLVGFPGYFTAVALLDKLGRKVIQIQGFTMMAIIYLLVSAVMIVSKGHKVSGFLIPAEAAFLLYALSFFFIDFGPNTTTFVIPAEVYPVRFRTTGHGISAASGKLGAAITTYLFPTLLTSMGIKNLLVMLAVVSIIGALLTFFFVPETKNKSLEEVAKEELVTPTTTAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4U2</accession>
    <name>F4B4U2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775024.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4U2"/>
    <dbReference type="STRING" id="933801.Ahos_0215"/>
    <dbReference type="GeneID" id="10599655"/>
    <dbReference type="KEGG" id="aho:Ahos_0215"/>
    <dbReference type="eggNOG" id="arCOG07335">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567184_0_0_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="46"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="190" mass="22217" checksum="A1BCC9184DA6CF87" modified="2011-06-28" version="1">MSSIFDSINSWNYRPFRRMGVTICEELRIGKLISSIIKRINPKKVLEIGCGNCLVTLSVEKETKLPSLVSIEVWNEEITDKEVKDYLKGEDYNLVENLFPLPFKEKSFDLAYSVLYFYNKIRKERSDLANEVSKVIKDNGYFILIEPEIVRNMRKDFFNAGFSEVEYHVDQGIFFSLMKKVDTNIQRSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W6</accession>
    <name>F4B4W6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thiosulphate-quinone oxidoreductase (TQO) small subunit DoxA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4W6"/>
    <dbReference type="STRING" id="933801.Ahos_0239"/>
    <dbReference type="KEGG" id="aho:Ahos_0239"/>
    <dbReference type="eggNOG" id="arCOG05342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1544265_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011636">
        <property type="entry name" value="DoxA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07680">
        <property type="entry name" value="DoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Thiosulphate:quinone oxidoreductase small subunit DoxA" evidence="2">
        <location>
            <begin position="31"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="170" mass="18673" checksum="98A6AEF2C1FC1A24" modified="2011-06-28" version="1">MVIEMEKEVALALLGFLIATGMTLWLYQIDFQGLGHLTNWSKTVGYCLEGTKLFTNGTLFLQISRVDGPDTYGGFIVLVQVLYPNGTVVYQWNANQLGHIPKSDIHNEFSLHPAHSTGFALCVPLGQNATVTLNMPFNISPGTYIVKVYDANGASAAYGQKWEIEVQAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4X1</accession>
    <name>F4B4X1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">succinate dehydrogenase</fullName>
            <ecNumber evidence="5">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Ahos_0244</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4X1"/>
    <dbReference type="STRING" id="933801.Ahos_0244"/>
    <dbReference type="KEGG" id="aho:Ahos_0244"/>
    <dbReference type="eggNOG" id="arCOG00962">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_3_2"/>
    <dbReference type="OrthoDB" id="144910at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000003">
        <property type="entry name" value="Succinate dehydrogenase iron-sulfur subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050573">
        <property type="entry name" value="SDH/FRD_Iron-Sulfur"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004616">
        <property type="entry name" value="PRK05950.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009226">
        <property type="entry name" value="PRK12576.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921:SF29">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] IRON-SULFUR SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11921">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="13" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="15">
        <location>
            <begin position="3"/>
            <end position="94"/>
        </location>
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            <end position="165"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93136.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="233" mass="26451" checksum="83DC8722CE10FF58" modified="2011-06-28" version="1">MSFEITLKVRRYSKEKGSRWQEYKLKVDRFTTVVEALRRIKTEQDPTLSFRASCHMGVCGSCGMKINGKPKLACKTLIVNEGKKEITIEPMDNFPVIKDLIVDLDSVYRKMNKIIPRVTPPEEVIQGKAETRLKPEDQKELWSFAQCIICGLCYSACPVVETNKDFLGPAVLAMVYRFSADPRDTLGKKRLEIVDDPIIGIWNCRVAGSCSFVCPRNVDPSLAIQRLKEMSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    </dbReference>
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    <evidence type="ECO:0000259" key="1">
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    </evidence>
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</entry>
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            <dbReference type="PubMed" id="21607549"/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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            <begin position="9"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18765"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93160.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="133" mass="15791" checksum="C21B54BD19F81A6A" modified="2011-06-28" version="1">MDLQDYLDKLKAFNWKDYNIYYAVLFGSLAKKGKGNDIDIAVEFKELSFDNYMKLWKDLSDYLDTEKIDLVSIDDNTSCYLIHEVFNYGLILYKEDWSRAHRRAVICEDFLIDAKKLDLVGREVRAVIRKWQS</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B535"/>
    <dbReference type="STRING" id="933801.Ahos_1454"/>
    <dbReference type="GeneID" id="10600950"/>
    <dbReference type="KEGG" id="aho:Ahos_1454"/>
    <dbReference type="eggNOG" id="arCOG00271">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088838_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF76">
        <property type="entry name" value="EAMA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="5"/>
            <end position="24"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="213"/>
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            <begin position="220"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="263"/>
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    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="133"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="29064" checksum="B870C832084BB98B" modified="2011-06-28" version="1">MNKSYLILIIGGISFGTAAIFIKFSDLTPGAIAFFRFLVAGLILSLGNIDLKKIIKYSPFGLLLSLHMITFILGVYNTTIIDATVLVSTSPFFAILLSPLSKFKAERIDIIITTIGFSGIIIMNYPLQIGYIYGNIMSLISAFLISLYTTLLSRNEENPLVLTSSIYISSSIFSLPFMIYQGIGKIDSTSVLSLLGLIFIPTLIGHTSVIYASNKVKPQYIETIGLLEPVVASIIAIFTFNQIPTLFEVLGSILIVSSIILVINKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B537</accession>
    <name>F4B537_ACIHW</name>
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        <recommendedName>
            <fullName evidence="3">CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein</fullName>
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            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776254.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B537"/>
    <dbReference type="STRING" id="933801.Ahos_1456"/>
    <dbReference type="GeneID" id="10600952"/>
    <dbReference type="KEGG" id="aho:Ahos_1456"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1673882_0_0_2"/>
    <dbReference type="OrthoDB" id="34360at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18369" checksum="C122351854CD9225" modified="2011-06-28" version="1">MIIKIHGVKGGSGKTTISKYLFYYFRKKERKRVSLHSVEEIVKCDNPEIIILDNVNLTIKNGNIEWKLFVTDPQSLELSLNYVKKDDDFIIVNKVSPFPCEQNEIIKKVYKFRSVLVPFNGKLFYDEYDELAEPTLNRLAENLLGLRKDRLIVPFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B562</accession>
    <name>F4B562_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="11">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrS</name>
        <name evidence="13" type="ordered locus">Ahos_1481</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776279.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B562"/>
    <dbReference type="STRING" id="933801.Ahos_1481"/>
    <dbReference type="GeneID" id="10600981"/>
    <dbReference type="KEGG" id="aho:Ahos_1481"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_2_2_2"/>
    <dbReference type="OrthoDB" id="372136at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000002">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000001">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="135"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="542" mass="62491" checksum="FD00D268E65457D1" modified="2011-06-28" version="1">MEEYKGVWLKGGIILAFNLLADGKKAVAVGLGERDFYVDVEMDSSMSLAEAKKYAKEGNYEIKIEGKKARAGNYEVSLESDVTPSGNPKYFEILNISVHHPTPDKQYVRIRGVAFETEEQLKDYMNWLEKASETDHRILGEKMDLFSFPEEAGPGLVLYHPKGQIIRNELIDYMKEINASMGYQEVYTTHVYRTILWKISGHYEMYKDKMLIFTHDDEELGIKPMNCPAHILIYKSRTRSYKDLPIRFSEFGNVYRWEQKGELYGLLRVRGFTQDDGHVFLREDQLENEVKMLISKTLEVLAKFGFKGDDVRINLSTRPDESIGTDEQWEKATNALVNALRSLNLKYQVKEKEGAFYGPKIDFDIRDSLGRWWQLSTIQVDFNLPERFKLEYVDKDGSKKRPVMVHRAIYGSIDRFLAILLEHFKGKLPTWLSPIQVRILPINPEVREYAEKVLSKLVSVKIRAEIDSEEETLSKRIKKAYDDGVPYILIIGKKEAESNSVTIRARGNIEVKGVPLDKCVDAISKEIQNRDLTNTAIEKVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B576</accession>
    <name>F4B576_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF302 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775086.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B576"/>
    <dbReference type="STRING" id="933801.Ahos_0279"/>
    <dbReference type="GeneID" id="10599719"/>
    <dbReference type="KEGG" id="aho:Ahos_0279"/>
    <dbReference type="eggNOG" id="arCOG02761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116237_3_2_2"/>
    <dbReference type="OrthoDB" id="157520at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14797">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="entry name" value="TT1751-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342:SF2">
        <property type="entry name" value="INNER MEMBRANE OR EXPORTED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38342">
        <property type="entry name" value="SLR5037 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="TT1751-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="121" mass="13785" checksum="B872745D6834A13D" modified="2011-06-28" version="1">MIIKECKFSFEECEKIIKDKIKEVGEIFAEMDHSKNAEKVGLSLPKDKVIIFGNPRAGTLLMQEKIEVSLDLPLRIAIWEKDGKTFLGIKMPSEIAREYGITHEIIKKMDEAVNYVISSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B4</accession>
    <name>F4B5B4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphate transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775124.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B4"/>
    <dbReference type="STRING" id="933801.Ahos_0317"/>
    <dbReference type="GeneID" id="10599758"/>
    <dbReference type="KEGG" id="aho:Ahos_0317"/>
    <dbReference type="eggNOG" id="arCOG02682">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF2">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="26"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="472" mass="52345" checksum="44D66A112EC1220E" modified="2011-06-28" version="1">MNWEDIKEKVFSEMNFMKSRGFHYRMLLLTGGGIFVDGYNIIIISYGLAGIQKAFHPSAYLLGLIGLSVIIGNLIGALIAGYVVDRLGRKTIFILDLIFFVGFAILSGLVTNAYELLLARILVGLGIGLDYPIATSYLSEFSPVRSRGKYLIMNITFFNIAGVVATPIAYWLLSYGELVAWRYMLMSAAVPAIIVLLARLGTPESPRWLLIKGRKEEAKKVIERVIGKPLNDEAAKLLDSVQITPEHSTYYKELLTKYTRDAIFIGVFYFLFAIAFLGTSIFGPEFESGLHLPGEISSMVFWSLFVVGDIIAILLVDRIGRRPITLIGWGGMTAMMLALIILPKSFTIGLELAFTLFAMFQGIGPASTHMVYSPELFPTRIRATAEGWKQGVGRLGGVLTGVFFPAISLDEKLYIILIASALGFIWSFLLAKETKGKSLEEISERTHVNISNTQLLNKDTTNNFPFNTEHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5B8</accession>
    <name>F4B5B8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0321</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5B8"/>
    <dbReference type="STRING" id="933801.Ahos_0321"/>
    <dbReference type="KEGG" id="aho:Ahos_0321"/>
    <dbReference type="eggNOG" id="arCOG06044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="entry name" value="GntR ligand-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43537">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, GNTR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43537:SF5">
        <property type="entry name" value="UXU OPERON TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="GntR ligand-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="9"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="222" mass="26033" checksum="D2387E1C22594ECB" modified="2011-06-28" version="1">MVKIIYSMSNLAEKAYHEILNAIVTGQYKPGQRLTEDQLCKDLNMSRTPIREALRMLLSDGVVKKDTKSYSVVYISAEEARMLYELRIPLEGHAAKLATMRANEEDIKKLGDILQRIKEEMEKENPDPLTLAELNGSFHEEVALLSKNKYLYNCLHDIRVKLKIVRTSLFTSYERRKDEYNEHYSVFLAIKEKDPEKAEKIMIEHEKKVLEFLEKRVFLFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5C9</accession>
    <name>F4B5C9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyl-hydrolase family 116 catalytic region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_027051_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
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        <property type="entry name" value="GH116_catalytic"/>
    </dbReference>
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        <property type="entry name" value="GH116_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052566">
        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654">
        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12654:SF0">
        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04685">
        <property type="entry name" value="DUF608"/>
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    </dbReference>
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        <property type="entry name" value="Glyco_hydr_116N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl-hydrolase family 116 N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl-hydrolase family 116 catalytic region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="74963" checksum="C18A08179125DA2B" modified="2011-06-28" version="1">MKLANNFGIPIGSLGTGKIDFFNDLTIGNATIMNNWSNPLRVIRGFHIVDLSTSMFLQGNPARNSEIKFEVKIPKSIEANALFPEVEYEIKNPDFTIKVYSPFVPNDLKDSSLPLIVFNIKGKGIIAISFPNLTGSRRWGRVNYKIEGKVNGVLMTNLKALQSDPAYGEIFLGCQGCHSYVGYRYWIPALKEGMTEDVSIFNLDKLKEGEGINRYYIKPYAREEIGGIVWKEIDGEENFYLTWFFNGRPGYYPYGHYYENWFQSAVDVAEYALKRKPKVELDNTQDWINDAVRNSMYVLTYSWLTKDGRLAVYEDPQISLLMNTIGGMTWDSLSFALLEYFPELVKKMDEHFGNFIIDGEVPHDLGEESIENPIYGASYPYSWNDLGPTWILMIYRDYKFTNDLSFLKRNYNKMKEVIDWLIKKDEDNDGIPDSKGGFDNSYDGTYMYGTSSYIGSLFLCALKAFIESSKILSYDYSKYEEILNKAKSSLESLWNGRYFINWKYKDQKNESCLNSQLLGEFWCNLLGLGNVIDEDKIKTALKYIYEHNGKASKYCLVNSVNPDGSIDESTDQMKSCWPRISFAIASHMIMKGMINEGIEIARKEWETISSRYPYNQPSKINAFNGEHFGLPYYIGSLSIYLVKYAIKNTPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H3</accession>
    <name>F4B5H3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776312.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H3"/>
    <dbReference type="GeneID" id="10601014"/>
    <dbReference type="KEGG" id="aho:Ahos_1514"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_34_0_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42718:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="7"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="482" mass="52376" checksum="257AA9F354949832" modified="2011-06-28" version="1">MDIKSRTLLILSLMLLVVNYVETMVIPALPTIEQDFSISPTLAGWVTSAYMIVAAATSPLMGKLADTYGKKKMYVTAIGFYIIAVAMAGFSPNIWVLLGARAIQGVGFSMFPIAIAYITDLYPKEKVAFAQAVLSAMIGIGPALGLLIGSYVVEDLGWPYAFHTAAILSLILFIISIKYLPHTGIRYKEKVDYLGSTLIGLATVLVLVYITEGPTLGWASVCNLSLLISGLVTYGIFIAVERKIKEPLLRLDLFKIRNFAVANLVGIISGVGMFTVFIFLVYYSQLPSPYGLGLSIIQSGLLMSPVAFGMVIFGPMFGRALPKIGPKPILILGSLLSSLAYIMLVYYRSNPTEVLIDGFISSIGLVAVIIPLVNMVALSLPEQYRTTGMGMNALLRTIGGAAGPVVATSFMQTYQVPVIMVYDNQPLVLGFMPSSTAFNYIAFFGLAMMILTLIVSLWTKNYTFRGVAVQENKTHLTNEYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5J0</accession>
    <name>F4B5J0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein, DUF340</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776329.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5J0"/>
    <dbReference type="STRING" id="933801.Ahos_1531"/>
    <dbReference type="GeneID" id="10601031"/>
    <dbReference type="KEGG" id="aho:Ahos_1531"/>
    <dbReference type="eggNOG" id="arCOG01615">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078428_0_0_2"/>
    <dbReference type="OrthoDB" id="21422at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015661">
        <property type="term" value="F:L-lysine efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005642">
        <property type="entry name" value="LysO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35804">
        <property type="entry name" value="LYSINE EXPORTER LYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35804:SF1">
        <property type="entry name" value="LYSINE EXPORTER LYSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03956">
        <property type="entry name" value="Lys_export"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="294" mass="32367" checksum="AA590F216273B946" modified="2011-06-28" version="1">MFFTLTFISLYAVSLILGRKINIPQIFSDGTVLILIFTISLWGGNQISAEYVGSIILVSFISSLVIVTITYFLGSFINERTEEGKIKGVDIKTQLKYLLPLIIGFLVGLITRFYSIIISDSVFDEVIDWELYVLAVVIGISIGKELKKELMKRISRFAIFSVIIAIIGGIISSAILFLFLSIRPFNLALAISLGSGWYSYTGPIVAKYYGPIYGVIAFLVNFLREQFTFSLLPIFLRIRSTPLGAVAVGGATSMDTTLGFYVDVLGYEYGVGAMINGVVLTLIVPLILPIILSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5R0</accession>
    <name>F4B5R0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775191.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5R0"/>
    <dbReference type="STRING" id="933801.Ahos_0386"/>
    <dbReference type="GeneID" id="10599826"/>
    <dbReference type="KEGG" id="aho:Ahos_0386"/>
    <dbReference type="HOGENOM" id="CLU_2021374_0_0_2"/>
    <dbReference type="OrthoDB" id="42872at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="122" mass="14481" checksum="91B767C979AE76B9" modified="2011-06-28" version="1">MHLIKLKTKIWHMLNEIEITYENVWNFLLNKKEVYILRASYIQPGKYICRHNTQEVECVIDQVIEANPMIMWQYLDKSGFSSLPEWMMSAAKVHMSHVRGGPFRKIMSLADKKYLLHVVLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5V9</accession>
    <name>F4B5V9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776372.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5V9"/>
    <dbReference type="STRING" id="933801.Ahos_1574"/>
    <dbReference type="GeneID" id="10601075"/>
    <dbReference type="KEGG" id="aho:Ahos_1574"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_9_0_2"/>
    <dbReference type="OrthoDB" id="351177at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="242" mass="28179" checksum="2F40227913E99D69" modified="2011-06-28" version="1">MLSIVIPAFNEEKRIGNTLNKLTSWFSSSEIIIVFDGNDNTPKVVKEYNAKLYVSKERLGKGASLKKGIEFSSYKKILLIDADLPVTRDDLNKIITTDADLVITTRRIIGMPWKRRFLHHSFIILTKIFFPSLRKFKDFQSGVKLVNREKALSVLDNLVINDFLFDINLIYEFKRRGYSIKEVEISYIHDETDSKISRKLFKIIILMFLSLLKLRVYYSPFKGILKTRAFLKAQGFILKTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5W4</accession>
    <name>F4B5W4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5W4"/>
    <dbReference type="KEGG" id="aho:Ahos_1579"/>
    <dbReference type="eggNOG" id="arCOG07934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_210681_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="58" mass="7010" checksum="A47201B63953F2A2" modified="2011-06-28" version="1">MPSSKYKHMRIEDKDEKGEGYLVIESKEDLEEFRKMLIEAYYELNPDRKRPCETQSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B614</accession>
    <name>F4B614_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776427.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B614"/>
    <dbReference type="STRING" id="933801.Ahos_1634"/>
    <dbReference type="GeneID" id="10601134"/>
    <dbReference type="KEGG" id="aho:Ahos_1634"/>
    <dbReference type="eggNOG" id="arCOG08437">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_671970_0_0_2"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704:SF1">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="18"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="386" mass="44045" checksum="3579EDCC8C6D6DA2" modified="2011-06-28" version="1">MRLYIRRREEEKIRKIKNWTLIYGRRKTGKSTLVKKNLHMDYYVLIADSNSAITADDKVMKIDDAIKEVKETLYKGGIAVIDEFQRLPDIYWSMISNWDKNGILVIVGSSYGIVNKVFDRNSPLLGLFTPLEIDIISYEDVLTQVKDPVLSTIYRDPWIIPFIDSYEEFVDRLKEFSLISKGLIGEVFKEEERQLTDTYYKILLLLGEGIWKTSEISGIIQPKGGEGTISSMVNKLVKMGLVQKIPTLSRENYYKVKSPPLSLSLYAESKYAISETDAKINYLPIGKEVQFSVGEMLSKYFGGIQYYSPKEDIDVVIVKKKKPIWAFEIKMGEISTSEAKEAIKKMSKVAEKVGLISLKEKPPDYGDLSLGPKELLEIANEVVKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B623</accession>
    <name>F4B623_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5658 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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    <sequence length="92" mass="10641" checksum="CD6B5FECD580CE1D" modified="2011-06-28" version="1">MKITLIISLLAILSFFDVYTTLIGITNGFVEENILLSSLENNIYLLLSIMIFLKIIAIVAIYYMMKRKLCLPAYVLLALYIFVDLHNIFLLY</sequence>
</entry>
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                <person name="Shah S.A."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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        <property type="entry name" value="AMIDOHYDROLASE 2 FAMILY PROTEIN"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="304" mass="35585" checksum="86AEC6A9A9CC3AB0" modified="2011-06-28" version="1">MLRNYMKKLLGENFIDERNRRIKDDPANYVKFLMEDAKIRAFVIDEGFGKKEQEIPVKYRLLFRIESIINENLFSLPFEKAVEVFEETLRSKIKEENYAGFKSIIAYRTGLKIICDENLARNDFLDEEREWFGRKAKGFRDYLFCKTMEIAKELNVPFQVHTGAGDRDIKLELSRPSYLTELVRKYEGKIVLVHAGFPFHRESAWMSYLFPSVYLDVSQIFPFATTAGKDVLREVLQIAPANKVMYGSDAFEIPEIAWVSAKLFRKYIISIIEEMENEEIIDEKDSLTIIKMIAEDNATKIYDV</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="protein sequence ID" value="AEE93352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    </evidence>
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        <source>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Wang S.Y."/>
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                <person name="Shah S.A."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="STRING" id="933801.Ahos_0474"/>
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    <dbReference type="KEGG" id="aho:Ahos_0474"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_0_2"/>
    <dbReference type="OrthoDB" id="359241at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="124" mass="14658" checksum="D68A27652CD653BC" modified="2011-06-28" version="1">MVEKFWFNKSDEFYSIAKEMAERGHYWFSCFASQQSVEFLLKGIEMKYTGSHTFTHDLSELLQKVEKILGVKATEEVYQACDFLTPHYTISRYSMVTSYDKRKALECIKMSEIVRNWVNSQIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B697</accession>
    <name>F4B697_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B697"/>
    <dbReference type="STRING" id="933801.Ahos_0497"/>
    <dbReference type="KEGG" id="aho:Ahos_0497"/>
    <dbReference type="eggNOG" id="arCOG01259">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05344">
        <property type="entry name" value="BKR_like_SDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_5G02870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43639:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="267" mass="29318" checksum="41976829FAA7DA6A" modified="2011-06-28" version="1">MITSWRKMDLGIKNKRVIVTASSKGIGFATAKRFLEEGAKVVISSHDERNLVRSYEKLKEISDEVYYVKADLTKPDEVKNLIDKGSSILGGLDVLAYVTGSPKPGNIFELSDEDWYQGFNLLLMSAVVAVRESSRKMTKGGRIILSTSITLRQPLDNLDLSNVIRLSLAGLIKSAARQLAPKGILVNGVMPGWTLTERVDQLVKDKARREGKTEEEALKEIIKDVPLGRPANPEEIANVIIFLASSLSTYVTGAIIPVDGGYLKAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B699</accession>
    <name>F4B699_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0499</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775303.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B699"/>
    <dbReference type="STRING" id="933801.Ahos_0499"/>
    <dbReference type="GeneID" id="10599939"/>
    <dbReference type="KEGG" id="aho:Ahos_0499"/>
    <dbReference type="eggNOG" id="arCOG00144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_34_0_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17504">
        <property type="entry name" value="MFS_MMR_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF192">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="449"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="11"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="484" mass="52496" checksum="43F221F0A3A86458" modified="2011-06-28" version="1">MDYDLNYAKKALYIMAPLAIMVMYTEGMLIPSLPSIEREFNVTASQVSWVISIYLLSGIIMNPIAGKLGDIYGKKKILSIVIWIYALGVTLTGFSPNFIALIIFRAIQGLGLAMFPLAFSLIREEFPPNLVPKAQGTISAMFGIGSAISLPIGAYISQTLGWQYTYHTVIPLVIILAVLIIKEIRESRIRLQGYKIDYIGAGILGISLVGIIVGTTEAPSWGWTSPSTISLLTLSIVLFSVFIFFESKVKYPLISVNLLRRRNVLISNLAAVVAGFAIFMAYQSLTYLFEEPTPIGYNLDIFMTGLSLLPLALIQIIGAMSAGRSISLKGPKPILVLGSGILVPTYFITSLLVYNGSSTPIELIILISSISMLGAAFLNVALINLLTFSVEREVMGISTSINTVFRLIGGSLGPAIAGSIMDTFTTAIEVPVNYNNHLTYVVTNIPSDYSFSVIYIISGIVAVIMTILASLSSNITYKKLSTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6A0</accession>
    <name>F4B6A0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775304.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6A0"/>
    <dbReference type="STRING" id="933801.Ahos_0500"/>
    <dbReference type="GeneID" id="10599941"/>
    <dbReference type="KEGG" id="aho:Ahos_0500"/>
    <dbReference type="eggNOG" id="arCOG05912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1665578_0_0_2"/>
    <dbReference type="OrthoDB" id="43595at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="18184" checksum="97BF9ECFDA8D8737" modified="2011-06-28" version="1">MSFSPTTKGEYYMTVGIVAVVLILISLGLGPLAPLDEPKVYAYDGKIYNLGDAVGVSFSAFISLMVFIISAVILWGSKNPWYNIIIDSSAISFIFLNYLNYAVIYLTWHPQMYILPFFVEIIYNGAKALQFDLGQVVLIILLYRVYKIMKMPRPLFGSEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D6</accession>
    <name>F4B6D6_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">DUF8155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D6"/>
    <dbReference type="STRING" id="933801.Ahos_1679"/>
    <dbReference type="GeneID" id="10601179"/>
    <dbReference type="KEGG" id="aho:Ahos_1679"/>
    <dbReference type="eggNOG" id="arCOG02979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010535_0_0_2"/>
    <dbReference type="OrthoDB" id="36515at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="entry name" value="Glucose Permease (Domain IIA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058468">
        <property type="entry name" value="DUF8155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26482">
        <property type="entry name" value="DUF8155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8155" evidence="1">
        <location>
            <begin position="3"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="273" mass="31176" checksum="BBEEE5BE6C2B6B73" modified="2011-06-28" version="1">MLLSYFSSGFPSHVKGKALDLSSDDMEYFYSPFYGRIERIEKFVVGRPNRFAEVNYDYLILLRRENGKLIKILHVEPFITVGEEIKKGDKLGKFLINPYTGGDFLHAHIEGLRIKFPKLTKYDERGIGKVVNKCKNYFDVEIITYSTAGNLRGLGCCGGLLNASLPYAGYGGIIGIENVSEVKIGNMKFFIHRIKKRNVVMFEVRKGLIRNWEYSPSFKVLEGKPIYGLPLFEAVLSINSLPRVRFFIKNSKFNEGDEVDVWELINSSSYLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E0</accession>
    <name>F4B6E0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD family hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776475.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E0"/>
    <dbReference type="STRING" id="933801.Ahos_1683"/>
    <dbReference type="GeneID" id="10601183"/>
    <dbReference type="KEGG" id="aho:Ahos_1683"/>
    <dbReference type="eggNOG" id="arCOG05919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623454_0_0_2"/>
    <dbReference type="OrthoDB" id="34377at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19007" checksum="069CE8FC3DBFF32B" modified="2011-06-28" version="1">MKYAIWLDGVVLDVDLTELLYKKFKGDKDVNLPYTFNVHKDWEDFYSKIKGKDIVFLSPYDERTTREIIEKVFKNDVIKFIPSMSKPEKKCLGRLFSEFPEFQPLETAIIGSSPLDLLSARFYDSRIKVLCVNRYTSCAKYSPYLMANSLEELYDLMVKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E9</accession>
    <name>F4B6E9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heterodisulfide reductase subunit C (HdrC-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776484.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E9"/>
    <dbReference type="STRING" id="933801.Ahos_1692"/>
    <dbReference type="GeneID" id="10601192"/>
    <dbReference type="KEGG" id="aho:Ahos_1692"/>
    <dbReference type="eggNOG" id="arCOG00966">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093432_0_0_2"/>
    <dbReference type="OrthoDB" id="144910at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF2">
        <property type="entry name" value="HETERODISULFIDE REDUCTASE RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="98"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="230" mass="25692" checksum="36B536E0DE45CBCD" modified="2011-06-28" version="1">MPIPELEKPIIKGVIEKEKVVVDGIELDGTWNAFLVERTQTGYDPSVWDEIANTLEGVTISACWQCGTCTSGCTMREYDPNFGPRKFIDLARKGDKQALAELQDSLWRCVSCQKCTHRCPKGVMVEEVVHAIHNYALKHGLVKKDPGTVFDELFLDTVMKNGGRISELLLGSASAKAGFVTMSLRDLITMSVPLIKSGLIKDLLKPNKVKNWDRIKTVLEEAMKEEVRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6F9</accession>
    <name>F4B6F9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutaredoxin related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1702</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776494.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6F9"/>
    <dbReference type="STRING" id="933801.Ahos_1702"/>
    <dbReference type="GeneID" id="10601202"/>
    <dbReference type="KEGG" id="aho:Ahos_1702"/>
    <dbReference type="eggNOG" id="arCOG01218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507297_0_0_2"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054067">
        <property type="entry name" value="SSO1120-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170">
        <property type="entry name" value="GLUTAREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21873">
        <property type="entry name" value="Thioredoxin_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SSO1120-like N-terminal thioredoxin-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="102"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="178" mass="20618" checksum="DC91B35A77646979" modified="2011-06-28" version="1">MSYDPIIKQYSNVIKGLKIEECLADELFEEMKVNNEIVKIEGCNRPVIKVEKEGRRFFTYYGVPEINELWPFLNALVRISSNTVHLQADEMELAKKLKGNLKLFVTATCTHCPAVAELFYQISIVNESVNLEIYDVDVYEEYIDKYHVLSTPKIVYNEKEFPSFPPLILLKMLAKSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G1</accession>
    <name>F4B6G1_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">Histone deacetylase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776496.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6G1"/>
    <dbReference type="STRING" id="933801.Ahos_1704"/>
    <dbReference type="GeneID" id="10601204"/>
    <dbReference type="KEGG" id="aho:Ahos_1704"/>
    <dbReference type="eggNOG" id="arCOG00326">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_0_2"/>
    <dbReference type="OrthoDB" id="147549at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="339" mass="38045" checksum="E3916CBD1AD5E2F4" modified="2011-06-28" version="1">MIGVIWDDRFLNISFSHPMIRDIAKARIRKFRELLEGIKDEILFIKPEPAKEEDLLVVHSKEYLRKLKEASNSPYIGFLDEGDTVHYPGMFDDVLLILGGSITAIKFSSFLDFIYVPLGGFHHALPCRAMGFCPINDIAIAAKILSKTRRVAILDVDAHHGNGLQRILYSDEVLKINIFGYDGHFFPGDGKMDEIGEGKGKGLNLNIPLPRGSGDDAFAEALKVTQVLEDFRPDYLLVNAGVDGHKDDSLHFLNLSANSFNYLGQKVRRLQRELNFKVISYGGGGYGESSALCMFEYILGYLGKKDNPEEETMGNAEIVRQEVDEILKTFYSSFVKNHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L5</accession>
    <name>F4B6L5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L5"/>
    <dbReference type="STRING" id="933801.Ahos_0537"/>
    <dbReference type="KEGG" id="aho:Ahos_0537"/>
    <dbReference type="eggNOG" id="arCOG03660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726883_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF1">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="377" mass="41664" checksum="3620B9BCA967A182" modified="2011-06-28" version="1">MAITLALITITSRATNNMVTTTLAPLGKYVFGFTNVVAGLLEALIYMSTFISTSYLNPRMNARVRRNAFIASNLAIAIILVLYYFSNAELIWLITPLAGISFGLILPNLITSASLVEDKKTAERLLGLYSTSLSISLIIGPSLETYILHLTGSYKMVFLAFTPLALIGAGIAWTIKFPNVKREKYGLSALKNKGFITSVLSITTYNVPFAAFTAFLTIYAIEKFHVSSAIGYSSYIPFFALSFLTRSFMTIRPFNSLKYPLMISIIITAFGLGFMIFAPSFTSFLIMMALLGIPHGSIFPMSTVMIARATTQEERNAVNSYFLAYNNILFTSVPAIIGFLSEFIGLGYSMLLLEIPVIVSAVIFFVKYWSDPIINRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6N0</accession>
    <name>F4B6N0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0552</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775356.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6N0"/>
    <dbReference type="STRING" id="933801.Ahos_0552"/>
    <dbReference type="GeneID" id="10599995"/>
    <dbReference type="KEGG" id="aho:Ahos_0552"/>
    <dbReference type="eggNOG" id="arCOG00600">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243045_0_0_2"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="118"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="26624" checksum="8D9D170A5573F1CF" modified="2011-06-28" version="1">MQINGSKIISISCESTIDEAIFFMARNNIRRIVVYCNNKIYGIFTVDEALRNLLFKSEAKLRDVELKKAVKISSTDVVHVVKSMVENSVDSVIYDDKIITEKDVVFSYDFPKVNVNNIAKEALQITPYTNVISAIEIMVKNGIRHLPVIEKEPVGMLSARDILYYYAQKYTVDVPVMDVMNPHLICVNENYPMSEAVKVMKEYNIGSLCINKNKIVTLRDFIRYIFTTFQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6R6</accession>
    <name>F4B6R6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Methionine aminopeptidase</fullName>
            <shortName evidence="8">MAP</shortName>
            <shortName evidence="8">MetAP</shortName>
            <ecNumber evidence="8 9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">map</name>
        <name evidence="11" type="ordered locus">Ahos_0588</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8 9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775392.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6R6"/>
    <dbReference type="STRING" id="933801.Ahos_0588"/>
    <dbReference type="GeneID" id="10600032"/>
    <dbReference type="KEGG" id="aho:Ahos_0588"/>
    <dbReference type="eggNOG" id="arCOG01001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="OrthoDB" id="372008at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="10">
        <location>
            <begin position="9"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="297" mass="33005" checksum="8A02F4D12C0615E2" modified="2011-06-28" version="1">MTDEELKIIMKVGKIAAEARDAGAKMIKPGVKVLDVCEAVEKMIIERGAFPAFPCNLSINYEAAHYSPVIGDEKVIPEGAVVKLDIGAQIDGYISDTAVTVVLDDKYQRLADASKEALNAAISNFKPGVSLGDIGKVIERVIRMNGYSPIRNLGGHLIRRYELHAGVFVPNVYERNMGRIMEGNTYAIEPFATDGFGEVIEGKMETIYSAKSFVPKGLTDEEKQFLDVINKRFKTLPFSERWLSDLGDKEKVEKTLKNLVRKKALNSYPVLIEIRKGMVSQFEHTVYVGKDETIVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y6</accession>
    <name>F4B6Y6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776594.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y6"/>
    <dbReference type="STRING" id="933801.Ahos_1804"/>
    <dbReference type="GeneID" id="10601304"/>
    <dbReference type="KEGG" id="aho:Ahos_1804"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_0_2"/>
    <dbReference type="OrthoDB" id="38470at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="114" mass="13238" checksum="D9E051E01E9AC85A" modified="2011-06-28" version="1">MAKWFEKSDKEVAYLLLEKGYYPEACFHSEMAVELKLKGILVEATRAIIYTHSIKRLLKEVEKVKKINLSDKILDCADYLSYLGSRYPEEEIIELGRSEGEKCVRCMEEILSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z7</accession>
    <name>F4B6Z7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="5">PGP</shortName>
            <shortName evidence="5">PGPase</shortName>
            <ecNumber evidence="5 6">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0592</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="5 6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775396.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z7"/>
    <dbReference type="STRING" id="933801.Ahos_0592"/>
    <dbReference type="GeneID" id="10600037"/>
    <dbReference type="KEGG" id="aho:Ahos_0592"/>
    <dbReference type="eggNOG" id="arCOG01213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_2_0_2"/>
    <dbReference type="OrthoDB" id="120822at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07514">
        <property type="entry name" value="HAD_Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01487">
        <property type="entry name" value="Pglycolate_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000:SF8">
        <property type="entry name" value="HAD SUPERFAMILY HYDROLASE-LIKE, TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10000">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01144">
        <property type="entry name" value="C2.B.4:_PGP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00446">
        <property type="entry name" value="phosphoglycolate_phosphatase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="229" mass="25802" checksum="91F5FF112E029089" modified="2011-06-28" version="1">MIKLVLTDLDGTLTEDRGIYKVSLDAIKYLRLLEERGIKVALVSGNSYPVLRGLHNYLGFSGGVVAENGCVVFYKQKIRVCKPMDRQILEEFREKFKLKDSWQNDYRECDFGFTPPDITEEMIKWAEEKGLYINSSGYALHIAFKPAGKMVGVRKLIELHGVKKEEVIGIGDSLTDLDMFKEVGIKVAVGNAEEELKKEADIILNLKSGEGVKELVTMILEGKLNGRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B773</accession>
    <name>F4B773_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1818</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776608.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B773"/>
    <dbReference type="STRING" id="933801.Ahos_1818"/>
    <dbReference type="GeneID" id="10601318"/>
    <dbReference type="KEGG" id="aho:Ahos_1818"/>
    <dbReference type="eggNOG" id="arCOG11458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1880934_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="135" mass="15453" checksum="252C66CB99DBD251" modified="2011-06-28" version="1">MRYDIPSFWHTSLGFSIIVKPTYKVYKLHGLSETVDYGEMLAEYYNAELAYVPQGDIDVVLLKKGKPFIAYEVKNKFTEKDARKAIERIKDFGISRAGLIGVLEDPPNSEDSIGPERLIEIAKEIEKRNKNIREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7B5</accession>
    <name>F4B7B5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved archaeal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1862</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776650.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7B5"/>
    <dbReference type="STRING" id="933801.Ahos_1862"/>
    <dbReference type="GeneID" id="10601361"/>
    <dbReference type="KEGG" id="aho:Ahos_1862"/>
    <dbReference type="eggNOG" id="arCOG05401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130233_0_0_2"/>
    <dbReference type="OrthoDB" id="56816at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="17766" checksum="0B8194D66BBE6183" modified="2011-06-28" version="1">MSSNENEKDKKTVTIRGVDKSLYERAVMTARETGRTVGEIVNQALSSFLGLTSKATNAVTQVVQGVKETGKSFVQGFEDAKKDVVVISDVEELSVTRDEILQVGKPISFRNIKKLVLNVDQETLNYVDSIIGVDELVIPSTINKIMLLQKCKFVKKISTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C8</accession>
    <name>F4B7C8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Extradiol ring-cleavage dioxygenase III subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776663.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C8"/>
    <dbReference type="STRING" id="933801.Ahos_1875"/>
    <dbReference type="GeneID" id="10601374"/>
    <dbReference type="KEGG" id="aho:Ahos_1875"/>
    <dbReference type="eggNOG" id="arCOG01730">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976397_0_0_2"/>
    <dbReference type="OrthoDB" id="57333at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="6">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="126" mass="14647" checksum="EB71F083F5C50385" modified="2011-06-28" version="1">MKGYYISHGSPLLLIEENEWKETLRNLGKKIRKEIDPETAIIISPHFFSWNGKFLIETQEKLECIQDYYGFPEETYKYCYSAENDTDTVNNLLSTGLFTPDNNLGLDHGAWIPLYYNRLKASSRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7C9</accession>
    <name>F4B7C9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">OrfB transposable element protein, IS605</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7C9"/>
    <dbReference type="STRING" id="933801.Ahos_1876"/>
    <dbReference type="GeneID" id="10601375"/>
    <dbReference type="KEGG" id="aho:Ahos_1876"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726877_0_0_2"/>
    <dbReference type="OrthoDB" id="42545at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Cas12f1-like_TNB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051399">
        <property type="entry name" value="RNA-guided_DNA_endo/Transpos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040570">
        <property type="entry name" value="guided_TnpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01766">
        <property type="entry name" value="IS200/IS605 family accessory protein TnpB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30405:SF21">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="Cas12f1-like_TNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas12f1-like TNB" evidence="2">
        <location>
            <begin position="297"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="381" mass="44917" checksum="91E6E97B5626DE70" modified="2011-06-28" version="1">MARRVKAIRATVSMKIALSDSLLAIVNNYVKALRFTLFWLKENVPNPEEKGVLSKVHEELYTRLREEYNLPSKVAEDCYREALATYKGWYNNPKKGRFPRVYKPTVWLTPKASYNVDFNKMIVRIASVGELPILGYPRNLKDYLSWRMREARLVIKSGKAFLKVVFEKEEENVEPKESVAVDINMAEVVVGKDDKNYVRIPTRLEEVHHWKSLAENLQKKYSRRWKENKRILHRIHSFHLKAKRIAEDFARKVGKWVVEIAEDFGANVIKLENLRNLIKNVNRLPREFRDKLYLMQYRRLQYWIEWQAKKRGMTVEFVNPSYSSVSCPKCGQKMREVSHRWFKCSCGYENNRDVIAIMNLNGRGSLTLSTAPQMRDVNPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F8</accession>
    <name>F4B7F8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0676</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775479.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F8"/>
    <dbReference type="STRING" id="933801.Ahos_0676"/>
    <dbReference type="GeneID" id="10600127"/>
    <dbReference type="KEGG" id="aho:Ahos_0676"/>
    <dbReference type="eggNOG" id="arCOG04064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042134_1_0_2"/>
    <dbReference type="OrthoDB" id="15212at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06159">
        <property type="entry name" value="S2P-M50_PDZ_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="204"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="357" mass="39156" checksum="D125061518BD1ACC" modified="2011-06-28" version="1">MNISLYIFAIGFVIFWGLILAFRKKLEPKGFTVYPLFLMWKKSTRSLWFPRLASSKPFKIYEKIAMILGILAMIGGITMIYYVIFGLIFHPQSTTVRLEPIIPGVTISLSCLPYILLALGISVTLHELSHAVSATSNKINVKSGGFILLGIFPGAFVEPADEEFMTSSLPAKIKILAAGIAVNLILAGIFFPLAMFLPGYFSQGLVIEGVIPNSSAYNASIQAGDVILSVNGIRTNTFNSLTTALNQSTNYTIVLKAPNGSTIVKHAESTKHFLGVYVTYYFPPSVRPFLLFVTWMFIINFSLALFNAAPLIITDGGKIFTELLKKISSQNGEKMSMAIQAFLLMSLVYAIMLSISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H5</accession>
    <name>F4B7H5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein eS24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rps24e</name>
        <name evidence="5" type="ordered locus">Ahos_0693</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775496.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H5"/>
    <dbReference type="STRING" id="933801.Ahos_0693"/>
    <dbReference type="GeneID" id="10600145"/>
    <dbReference type="KEGG" id="aho:Ahos_0693"/>
    <dbReference type="eggNOG" id="arCOG04182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107248_3_2_2"/>
    <dbReference type="OrthoDB" id="27533at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="118" mass="13331" checksum="69916EC6D6E87A90" modified="2011-06-28" version="1">MSQAQQIKISDKIQGVIEKDFDNKVIRRRELTLKLYHIGTGTPSRNEIKKAISELLNTKEDLIVVRKVFTSYGAGISTARVHVYTNKETLEKYEPKHLLTRGTSTKKEGEQGGEKGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7L1</accession>
    <name>F4B7L1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="3">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pcn</name>
        <name evidence="5" type="ordered locus">Ahos_0729</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775532.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7L1"/>
    <dbReference type="STRING" id="933801.Ahos_0729"/>
    <dbReference type="GeneID" id="10600183"/>
    <dbReference type="KEGG" id="aho:Ahos_0729"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043978_1_0_2"/>
    <dbReference type="OrthoDB" id="14749at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002218">
        <property type="entry name" value="PRK01115.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="4">
        <location>
            <begin position="12"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="248" mass="27246" checksum="CF6D102303C7BE91" modified="2011-06-28" version="1">MFKVVYGSSLDFYRLITAMSKLVDSPILNLTEDGVVAKHLTDDKVLMGVINIPKDVLDDYEIEKPLSLKITISDLKKVLSKAKSKNSSIEISETDYGVKITIRDEKSGTRSTMNLKAEKGEPQVLKEPNVKLPVSIVFEGDILKTIISDASEIGEEVEFSAEGEEVTISTEESGKSYKAILKKDQPLKEVSIESSAKAVYSLEVLKTVATASSFTDTLKLSFGNNLPMKVEASTDKGAMLIFWVAPRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N8</accession>
    <name>F4B7N8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved TM helix repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776700.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7N8"/>
    <dbReference type="GeneID" id="10601411"/>
    <dbReference type="KEGG" id="aho:Ahos_1912"/>
    <dbReference type="eggNOG" id="arCOG01572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_996122_0_0_2"/>
    <dbReference type="OrthoDB" id="37156at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008910">
        <property type="entry name" value="MSC_TM_helix"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05552">
        <property type="entry name" value="MS_channel_1st_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="269" mass="29050" checksum="C6BCB61EA58302FA" modified="2011-06-28" version="1">MLPLVIYSISAAISNLETDFINAIPSIILFIITVIIGYIIAKLVSDVVARVLHNFSASYPELRIGTGLIAGTVEALIILIALAIAFSLLNLGPASVYVDYIARYLPYLAGAILLLTLGTSLVNLFTDFANRQIGQTDPFANVIIQVLRLGLYAVIITVAAELAIFYWIPSINSYLFYGIIIGSIILLFSFTITGKAIDEISKAHPETASVLGYARLVLYAVFILIAIGIIVQPFGNVTAIIQTFAWGLAIAFAIIVIPLVYALAKKIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Q1</accession>
    <name>F4B7Q1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776713.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Q1"/>
    <dbReference type="STRING" id="933801.Ahos_1925"/>
    <dbReference type="GeneID" id="10601424"/>
    <dbReference type="KEGG" id="aho:Ahos_1925"/>
    <dbReference type="eggNOG" id="arCOG06020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140060_0_0_2"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="8"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="113" mass="13472" checksum="BD3B15193BAE5D4F" modified="2011-06-28" version="1">MRKFEVLLKEANDDLERGNLNKAVSALYFAVRKRLECILLILRYEVPRRDDKLANVLKYLGYSEESKIFIELYELRKKADYSEELISKEEAKKALEYATRILKILEKLEERVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R5</accession>
    <name>F4B7R5_ACIHW</name>
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        <submittedName>
            <fullName evidence="2">DNA polymerase beta domain protein region</fullName>
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        <name evidence="2" type="ordered locus">Ahos_1939</name>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R5"/>
    <dbReference type="STRING" id="933801.Ahos_1939"/>
    <dbReference type="GeneID" id="10601438"/>
    <dbReference type="KEGG" id="aho:Ahos_1939"/>
    <dbReference type="eggNOG" id="arCOG01203">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131320_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05403">
        <property type="entry name" value="NT_KNTase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052548">
        <property type="entry name" value="Type_VII_TA_antitoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33933:SF1">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE MNTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="163" mass="19215" checksum="C2302DD305A47783" modified="2011-06-28" version="1">MVSEPYASLLNKMVSIMKEEFKDSLISVVLYGSVARGDNRKDSDVDLIVVIKDLPKDSMLKRIRLFETKVEDKLNLEEYWKKGYYITLSPVLKTPEEAVRFSPLYLDLVYDGIILYDKDNFFLNILEKLKEKLKELGAERVRQGKMWYWVLKKDYKFGDVIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7S9</accession>
    <name>F4B7S9_ACIHW</name>
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            <fullName evidence="7">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776741.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7S9"/>
    <dbReference type="STRING" id="933801.Ahos_1953"/>
    <dbReference type="GeneID" id="10601452"/>
    <dbReference type="KEGG" id="aho:Ahos_1953"/>
    <dbReference type="eggNOG" id="arCOG03654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031607_0_0_2"/>
    <dbReference type="OrthoDB" id="43026at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="22"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="445" mass="48865" checksum="2B30BE5E1FD2946A" modified="2011-06-28" version="1">MSKEKEESHYQEPKRVIGLKDLVFLSVGGQSPFLSILTYGVAAFLYGSYFAPIAIIIGTILVLLNGMVIYKLSIRYTKSGGYYTYAYYSLTKRLGFETGWIYLVYSVLYGSAYILGATFVLHHVLQINPWIIALTIFGVSSIFALTGIKPSAKYAIVASVIEIGIMATLAVLFLKSTSFTFYNPFNYHISFGMLALAILFGAGIPTGYGSITPLSGEVKDPKRTVPLAIVIVILLGGLLAAFDVYAIGDHLIYYHISPTSTDLLSLIESRFGIITFAFVIFAAANDGILATLSFMLATSRTAYAMSTHGFLPSIFSKFESERGPIFASLLAIVLYLITIVSSLYLAGLRPYLAFEEVAEIAVLSNLLVHIAADSSLFKISLKRFNKRLVEISLALAAIGITAWNMIETISSTTPVLVYIFMTFIIMGFLAAEVIEMEREEQQQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7U7</accession>
    <name>F4B7U7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AAA family ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1971</name>
    </gene>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7U7"/>
    <dbReference type="STRING" id="933801.Ahos_1971"/>
    <dbReference type="KEGG" id="aho:Ahos_1971"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1820911_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF19">
        <property type="entry name" value="ARCHAEAL ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4143" evidence="1">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="141" mass="16508" checksum="18E9AE360BDDC173" modified="2011-06-28" version="1">MVGRYKLRDVIKFKEFANSIVKYYSSEISIRKISYSLGFSTATVEEWLSYLQESYLVYPIKRYSRSPKQINGQRKIYVVDPGIVNYLAQFSYGVLMENVVAIHLLRKNQLEGVYYLRGEDYEVDFFDEKEGGVNSSIFCFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y2</accession>
    <name>F4B7Y2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0113 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775575.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y2"/>
    <dbReference type="STRING" id="933801.Ahos_0773"/>
    <dbReference type="GeneID" id="10600232"/>
    <dbReference type="KEGG" id="aho:Ahos_0773"/>
    <dbReference type="eggNOG" id="arCOG00993">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127467_0_0_2"/>
    <dbReference type="OrthoDB" id="36461at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0113" evidence="1">
        <location>
            <begin position="125"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="20227" checksum="3166B0D815F87A69" modified="2011-06-28" version="1">MSSQKMLVKTISAHELMKILEKIIMEFDCSYDEIYEHFSKCYIYEIIGEKFSQISLSTSDFSNIINVMSKIGLHPYMIGTPILRVTKSKKIFPLLPLGNILTKYCKKIIRLNENLINKIIYGKTIEINDKIGFNRAIIVNKYGEFIAYAKIKREKNYTLIIPELDIGWYLRKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B818</accession>
    <name>F4B818_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription factor E</fullName>
            <shortName evidence="4">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tfe</name>
        <name evidence="7" type="ordered locus">Ahos_0809</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B818"/>
    <dbReference type="STRING" id="933801.Ahos_0809"/>
    <dbReference type="KEGG" id="aho:Ahos_0809"/>
    <dbReference type="eggNOG" id="arCOG04270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00373">
        <property type="entry name" value="transcription factor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="178" mass="21056" checksum="DE58C48264A5FEEA" modified="2011-06-28" version="1">MTSNVDELILNLAKSLLGEEYTELLEFLLNKKTEMTDDEIANELGIKVNEIRKKLYALADQGLVSYRKSRDKDTGWYIYYWKVNTEQINEILLNRKREILEKLKARLEYESNNEFYMCPEDKSKYTFDEAFENEFKCPKCGSQLIYYDSSKVRQVLEKKINELQEEIDKETKLGAKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B868</accession>
    <name>F4B868_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776807.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B868"/>
    <dbReference type="STRING" id="933801.Ahos_2020"/>
    <dbReference type="GeneID" id="10601519"/>
    <dbReference type="KEGG" id="aho:Ahos_2020"/>
    <dbReference type="eggNOG" id="arCOG00132">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025894_3_0_2"/>
    <dbReference type="OrthoDB" id="56622at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23520:SF5">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="403" mass="43946" checksum="2E0185AC67DF7834" modified="2011-06-28" version="1">MSEDFGFKYLLISRIFRSIGLIFVTISSPLYLKALGYSPLLIGLVFSAVVGFTAILSLSLGFLGDRKGYKKSLIIAEGISTLGVLLLVITPTSSFALLSTSLILAGITGSATGMRGLFSPGLTALVATNWKEDFERVRKMGLLSSVASLSSIGGSLMLTLMDYLPFGKIGNYKFLFFISSIFLILSFISLLFVKENREKRRIKKEKIMKKSSLKYVSKVILSNSIGAVGIGMSIPLLSLWFSLFYHATDSEIGEIFTLNYIFTSLGSFLATRVKGNILRLASITRIMNGVFLITMALSPYLFLAGIMYILRGLNAGFGAPNRTAVNIKGISPEDYGTASSLQGLATRFSQMSSSIGGYLMEIDLPLPLEIGGLLQAISGIMYLRLFRKDVEERSINAETKTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B879</accession>
    <name>F4B879_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Receptor for ABC transport, TroA superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776818.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B879"/>
    <dbReference type="STRING" id="933801.Ahos_2031"/>
    <dbReference type="GeneID" id="10601530"/>
    <dbReference type="KEGG" id="aho:Ahos_2031"/>
    <dbReference type="eggNOG" id="arCOG04233">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_9_1_2"/>
    <dbReference type="OrthoDB" id="9784at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051030">
        <property type="entry name" value="Vitamin_B12-ABC_binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860:SF2">
        <property type="entry name" value="BLL4160 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42860">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="299" mass="33972" checksum="CEC47BFCED8B2504" modified="2011-06-28" version="1">MKRIVTLDPATTEIVAFLDGTSRIVGVPEDADYPEEVKQKVKVTRKLVNVDNSLPSEVIDEIVRQHIKERKPLHEALWNKIYELEPDLIVGQGICEVCALPSISSLGNEKIITTPKKYRISRMEVYNPSTFMDIPKETMKIAKILGRERKAEELKEQFEKSIEETKGIAKGTKTVVIEWIKPIHLIGKWVSDMVNLMGSKPLARPGGQGGVFDWEVIREFNPDYLIISPCSFSVNRSLMEIEKITTLPGYSDLNAVKQGHVYVLEPLYARASQRTLEFLNALKEIYTTGEVERKYGIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B882</accession>
    <name>F4B882_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Archaeal PaREP1/PaREP8 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776821.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B882"/>
    <dbReference type="GeneID" id="10601535"/>
    <dbReference type="KEGG" id="aho:Ahos_2035"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="OrthoDB" id="37218at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="20028" checksum="5806AD7DC2D1CED4" modified="2011-06-28" version="1">MEKDLYKVGEDYVEARIIESLSDLLLSLTLWKEGYTRNSAGKAFSAVKALMSAFIVTNEDKLINLAKDDQERKWIKKKAHVVPTHDMYGLAQLLRKIGIDIVNLVNYALDLHDYQYNGFEPGFSKYSKTEQVLEDLITVVKGTKKVIELYFAKYEIKEVSEKIDELLKELEEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8C5</accession>
    <name>F4B8C5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">HAD-superfamily hydrolase, subfamily IA, variant1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0838</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8C5"/>
    <dbReference type="STRING" id="933801.Ahos_0838"/>
    <dbReference type="KEGG" id="aho:Ahos_0838"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF2">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="201" mass="23116" checksum="897A328ECFFBEEBF" modified="2011-06-28" version="1">MSDYFNDISFSPGYEKILSILISISEKLDSEGVYDRNKWWEEALKELNLKVEKDQLYDWTSLYWSIASNNPVYDDAREIIEYLKSKGYKLGILTNDDGEGGNKLQRIKKVDISSFFDKIVIAGTNGVKPKPNIQPFVYICEQLNEKVENCTMIGDDPVKDCLAAKKAGLKSILIDRESKVKFAELYADYVIRSLRELEEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F3</accession>
    <name>F4B8F3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15</name>
        <name evidence="9" type="ordered locus">Ahos_0866</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775668.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F3"/>
    <dbReference type="STRING" id="933801.Ahos_0866"/>
    <dbReference type="GeneID" id="10600334"/>
    <dbReference type="KEGG" id="aho:Ahos_0866"/>
    <dbReference type="eggNOG" id="arCOG00779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="OrthoDB" id="9418at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="8">
        <location>
            <begin position="71"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="145" mass="16319" checksum="2D1A7B9BD977797E" modified="2011-06-28" version="1">MVVRKEKKSRKMRGYRTMGWGSKGQHRNRGARGGRQVGMHKHKWSWLVKYGKDWYGKHGFVNPTSVKISAISLRDLQTSIENGSIKIDEKDGKKIVDLTKYGFDKLLGSGNLNISGLSIIVRHATEKAKQKLEKIGGEVILTPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M2</accession>
    <name>F4B8M2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RecA-like, putative circadian clock protein, KaiC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776889.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M2"/>
    <dbReference type="STRING" id="933801.Ahos_2103"/>
    <dbReference type="GeneID" id="10601603"/>
    <dbReference type="KEGG" id="aho:Ahos_2103"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01124">
        <property type="entry name" value="KaiC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022443">
        <property type="entry name" value="KaiC-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03881">
        <property type="entry name" value="KaiC_arch_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF2">
        <property type="entry name" value="PROTEIN GVPD 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="3">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="259" mass="29638" checksum="9A5C7807556B581F" modified="2011-06-28" version="1">MARLSTGIPDFDKLIEGGIPQGFFVAATGEPGTGKTIFSLSFIHEGLKEGDIGIYVTTEESRDSILRQAKQFNWDLESYLDKKLIIIDALMKEKEDEWSLEEVTPEEMVKKVIAAKQKLGYGRARLVIDSVSAFFLDKPAMARKISYYLKRVLYKWKFTIIATSQYAITTSQAFGFGVEHVADGIIRFRRVVKDGMLHRYVLIEKMRQTNHDKYVWEIDIVPGKGIVLLGKVQERKEDYALPKKVMDKIKEANKDEFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q2</accession>
    <name>F4B8Q2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lysine biosynthesis protein LysW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q2"/>
    <dbReference type="STRING" id="933801.Ahos_0886"/>
    <dbReference type="KEGG" id="aho:Ahos_0886"/>
    <dbReference type="eggNOG" id="arCOG01588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195720_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd13946">
        <property type="entry name" value="LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005906">
        <property type="entry name" value="LysW"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041070">
        <property type="entry name" value="carrier_LysW_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393:SF2">
        <property type="entry name" value="ALPHA-AMINOADIPATE_GLUTAMATE CARRIER PROTEIN LYSW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40393">
        <property type="entry name" value="LYSINE BIOSYNTHESIS PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21344">
        <property type="entry name" value="Zn_ribbon_LysW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="64" mass="7056" checksum="B30B2B677879DB2C" modified="2011-06-28" version="1">MLLLFRPNMVTLKCPVCGGQVNVEDDALPGEIIEHDCGAQLEVYKENGKLALRLAEQVGEDWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8V3</accession>
    <name>F4B8V3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775739.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8V3"/>
    <dbReference type="GeneID" id="10600409"/>
    <dbReference type="KEGG" id="aho:Ahos_0937"/>
    <dbReference type="eggNOG" id="arCOG05946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654919_0_0_2"/>
    <dbReference type="OrthoDB" id="36791at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="404" mass="46129" checksum="32BD09B8B3AF641B" modified="2011-06-28" version="1">MKAYDLISYLLEHAENGSIAALTTEDNIPILLTKNGEYSFTAYICTQDGEVKTVKKTFDKTTFHRAVLDFIDEVEEYIGKEINDVKISDVALFTNCIPKREERKPREKRDNLLDMISELRKVSEPFYIVPLLSNQGKLIAYVPEIGATSYFDFMVNNVSIVNGKIEPASPDLKLLYLVLFTNKLDPHNGNPLTTLDNITFFTAVFIDNGDKGKGEFEGKSVNKRIGRFFLSTYKGGLRTEELEFFDLSSLNKGRLYAGLFVKKDEKILRIGGISLVDFHNSGKLEINEYLFASFSQSARNGILDFSNYDKLFSNFLNLAISKSDARSLLKDVIEIHSMMTDMPFALQNVNNQISIVDPISFWYYSIKGEDIKECNDCPLKDKVNLRKEIFNTLRRRGWLNAFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B933</accession>
    <name>F4B933_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776971.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B933"/>
    <dbReference type="STRING" id="933801.Ahos_2185"/>
    <dbReference type="GeneID" id="10601688"/>
    <dbReference type="KEGG" id="aho:Ahos_2185"/>
    <dbReference type="eggNOG" id="arCOG01391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_644993_0_0_2"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050321">
        <property type="entry name" value="Glycosyltr_2/OpgH_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43867:SF2">
        <property type="entry name" value="CELLULOSE SYNTHASE CATALYTIC SUBUNIT A [UDP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="8">
        <location>
            <begin position="52"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE95056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="432" mass="49867" checksum="C810E611947A103C" modified="2011-06-28" version="1">MQVSIFLGFLLVFSSLLSSSWLVLQVYYIKSIKGEEIENRIDLQSQNNHKISIIIAIRNEDTDTINELIQNLRNINYDNYEVIIVSDDPPEKFREILSNISKIPENVKIIRRSENKGRKAGALNYGVNISSGEYLVFLDAEARVENDFLIKISKYLTQFDAIAMRLRVRNKENCVENVYYQMTEHSMNALFLARDRKNFIIFPNGSAFAIKRRIFERIGEWKEGAITEDLEMGIRLALSNVKVKFINNIVIYTLSPFTLSDLYFQIKRWAYGSGELFFEGLLLLRKGLRGLEGFLYVIQWGIYSAFIFFLVLTSVLGFIVNISPYFYTISILIYTISILVYSINFKVNSYNLHKISSVVLWSSLIGFIQGFLHIGTKWRVTPKTNKSKDELPISIKIFGILLFIISFLELLKGLIFESLILFSLFLSIAIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B934</accession>
    <name>F4B934_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="7">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="7">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ppa</name>
        <name evidence="8" type="ordered locus">Ahos_2186</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776972.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B934"/>
    <dbReference type="STRING" id="933801.Ahos_2186"/>
    <dbReference type="GeneID" id="10601689"/>
    <dbReference type="KEGG" id="aho:Ahos_2186"/>
    <dbReference type="eggNOG" id="arCOG01711">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_0_2"/>
    <dbReference type="OrthoDB" id="134160at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000003">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002317">
        <property type="entry name" value="PRK01250.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="19413" checksum="30E57AEA572D9C7D" modified="2011-06-28" version="1">MKVTPGSKAPELVNAFIEIPMGSNIKYEYDEEEEVLKVDRILYTSMFYPFNYGFIPGTMSEDGDPLDILVISNYPFNPGTVVEARPVGLIYMTDEEGVDKKIIAVPKDKVDPTFSNIKDVTDIPDAIKNKLVHFFEHYKELEPGKWVRISGWGTAQEAKAEIKKAIERNHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B979</accession>
    <name>F4B979_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_0986</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B979"/>
    <dbReference type="STRING" id="933801.Ahos_0986"/>
    <dbReference type="KEGG" id="aho:Ahos_0986"/>
    <dbReference type="eggNOG" id="arCOG00009">
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            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="555" mass="60438" checksum="44BDFBA103C1A17B" modified="2011-06-28" version="1">MFVLNKQFISTQLLFKLHKTYIKATRIFLPMSGGRGLKKEIGLKSLIIIGISSAVATAIFFSPLEMSAVAGPGSMFSWLLGIFFYIMISITYIELSQNYPEAGGPSRFSIYSHGAVTNLINAMADLVWYIFIPPIEAFATIEGLSFIFPSLLFNGVPTIEGAIVGVLLMLGYIPFNYYGVKTFAKVTSAFGIIKYIIYILPAFLLLFVYYNPANLTAYHGILPFGVSGVFAAMPYAMFAFGGARVIPDLAEEVKNKAYLIYALIITVVTEGIIYLFFNFVFLTNLDWSKIGVTAGNWGGLSHVQGNPFIDLAYDHDAQIALILLLIGGIIGPFLTGYVYMGTGSRVLFASARSGFVGNKMLELHEKYAIPYWALIVFAVVGSAIAFLFAPVPSIYGLISDATVAGYIGFATNPVALLVLRKQGATNYKVTGGSIIAPIAFMASGLIVFWSGWPAVPYSVVILAAVSGVLGVIGKVKEGLAESLWYMGYIAFLTFMTYIGSDGALNLINYDMSTIIVALVSLLVFYPLGIVQGLRQRNFEVHKEATSNQESEEEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B987</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_0994</name>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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            <begin position="6"/>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="175" mass="19508" checksum="A2DF66B29B48CE22" modified="2011-06-28" version="1">MRSIKISIPVSLVTWWLPLVGGAIIGFISGTIERKIASAIISVGITSAIASTFYILLGFKVLYVPLLGNLLPVILVISSVVNSALAVFVAYFISTKMIRSTFEGNNAKIEFYARDQDEINEKLNEIATQCSEPTYSFHSENEVEVTRRCEGFTMRYIIRKEGKLFKVTLFINGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A7</accession>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="10600488"/>
    <dbReference type="KEGG" id="aho:Ahos_1014"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
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        <property type="entry name" value="SQRD"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="211"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="409" mass="45369" checksum="BEC1F9CCCE432557" modified="2011-06-28" version="1">MTKVLVLGGRFGALTPAYTLKRLVGSKAEIKLINESRFSWLRLDLPHVSIGVKDVDEFKLDLSQALPEKGIEFQEGKVIKIDAKNNEVIYKKPDGSIEEEEYDYVIVGIGAHLGTELIKGWDKYGYSVCEPEFALKLRDRLATFQGGNITIGSGYFYQGHNPRPKVPENLAPFSDAACEGPVFEMSLMLHGYFKKNGMLDKVKMTVYSPGEYLSDLSSDARKAVGEIYKEFGITLIQNFRVREITEHEIIDEKGNRIPSDLSIILPPYTGNPALKNSTPDLVDDAGFIPTDLNMVSIKYDNVYASGDANAITVPKLAYLAVKTGRIAAQHLANRLGVPTKVDKYDPAVVCIADNPLEGYGVAVSDNTMYKGTHSTAVPSPVNSLKKELFVKYFMWSKGDLALEKYFGEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B3</accession>
    <name>F4B9B3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Daunorubicin resistance ABC transporter ATP-binding subunit, DrrA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_1020</name>
    </gene>
    <organism evidence="6 7">
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        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Drug exporter-1 (DrugE1) (TC 3.A.1.105) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775822.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9B3"/>
    <dbReference type="STRING" id="933801.Ahos_1020"/>
    <dbReference type="GeneID" id="10600495"/>
    <dbReference type="KEGG" id="aho:Ahos_1020"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043215">
        <property type="term" value="P:daunorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900753">
        <property type="term" value="P:doxorubicin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005894">
        <property type="entry name" value="DrrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DrrA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01188">
        <property type="entry name" value="drrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43582:SF2">
        <property type="entry name" value="LINEARMYCIN RESISTANCE ATP-BINDING PROTEIN LNRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DrrA1-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="323" mass="36275" checksum="D60D4E72E225F260" modified="2011-06-28" version="1">MSIVIAKNLTKKYGDFVAVDRISFEIEKGEIYGLLGPNGAGKTTTIKMLTGLTPITEGDAVIAGISVKKKPREVKKRIGWISSEVILDDELTAWENLEIQAKLLGVKDWQERADQLLEYFGIKEFKNRKAGKFSTGMRKKLEVSMALLHSPEIIFMDEPTIGLDVNTRASLWNLIRQINKDYQVTILLTTHYMEEADMLCNRISIINRGKIIAQGTPEELKEKYGGDVIEIELKEAKDGVLSDLESVGKIIKSEDKKIAIKVGNAEEVLLKVIGIVGQNNIKSLKINKSSLDTVFLNLTGGSLEENEEFDARRFYAMLRRARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9C3</accession>
    <name>F4B9C3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9C3"/>
    <dbReference type="KEGG" id="aho:Ahos_1030"/>
    <dbReference type="eggNOG" id="arCOG07280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1329395_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="206" mass="23895" checksum="C9EB0686A4B00C6C" modified="2011-06-28" version="1">MNLSLQFYIQGILFKLLQKYSSSNCFVYSVSTNSPFSFSGTSGRIKIRGEFRRKIKLHFSCGSVTSHVEYDLPRSMDELAKTVQEIEKEAEEKLHEVINNCELISLCETISGKTKVHVIKGKTLNLNDELKEEISFALFKHYGGRKVFFNLSEEEGIHVVNVIKNDKNKVYIQLFSPNQWKFWYLSEDYVVDEDLYAIMKKIHNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E1</accession>
    <name>F4B9E1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methylmalonyl-CoA mutase, large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777002.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9E1"/>
    <dbReference type="STRING" id="933801.Ahos_2216"/>
    <dbReference type="GeneID" id="10601721"/>
    <dbReference type="KEGG" id="aho:Ahos_2216"/>
    <dbReference type="eggNOG" id="arCOG04232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_2"/>
    <dbReference type="OrthoDB" id="38408at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03680">
        <property type="entry name" value="MM_CoA_mutase_ICM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.240">
        <property type="entry name" value="Methylmalonyl-CoA mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101:SF1">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48101">
        <property type="entry name" value="METHYLMALONYL-COA MUTASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="Cobalamin (vitamin B12)-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylmalonyl-CoA mutase alpha/beta chain catalytic" evidence="2">
        <location>
            <begin position="30"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="551" mass="62828" checksum="4CE0F2BBDF2DD87D" modified="2011-06-28" version="1">MDIDEKIKEWNNLYTSWIAKRKERKQKFITPSGIEVKPLYTPLDLKGNYVDKIGFPGMYPFTRGIYPNMYRGRIWTIRQYAGFGSAEDTNLRFRKLLEAGQTGLSMAFDLPTQLGLDPDQELAFTEVGVVGVSMFHWKEMDLVMNQIPMDKVTTSMTINATAMELMSMYIATAESRGIDKKVLDGTVQNDILKEYIARKNFIYPPEPSLRYAIDLIEYSYKNIPKWHPISISGYHIREAGADAVLEVAFTLADGIEYVRKTLERGINVDDFAPTLSFFFAGYTNIFEEVAKFRAARRMWAKIMKEWFNAKKPDSMTLKFHTQTGGAELTAQQPEINIIRTTLQALAAVLGGTQSLHVNSYDEALALPSEKAAKIAIRVQQIIAYESGATETVDPLAGSYYIEWLTDEIEERAWKVIDNIEKMGGMLKAIERGYPQAEIAESSYRLQKRIEEGDIIKVGVNFAYESDWIGTTEIFRVNPAVRERVISRLKQYKENRDNIKWKDSLEKLRKAAEGNDNLFPYVLDAIKAGATIGEISSVLREVWGEYKEPIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F8</accession>
    <name>F4B9F8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine synthetase catalytic region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_2233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F8"/>
    <dbReference type="STRING" id="933801.Ahos_2233"/>
    <dbReference type="KEGG" id="aho:Ahos_2233"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_6_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="4">
        <location>
            <begin position="104"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE95104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="423" mass="47604" checksum="44F70F8350B2C72B" modified="2011-06-28" version="1">MLEILKSGRVDYVRVEFIDLLGNVRGRSLRRAEFENLILKDSGLPYPESLLMLDYADSPIKSRYGDVLAIPDPSTFIILPYLERTARVLSYITLPDLSPSPFCSRGLLKRAIDKLEEKGLSIQVSFEPTFYLVKENGNGISPADEAKAFSPEGLMEEQSFLRDVIKYLESVGVQVETINKHYGPAQYEIRFAEKDALSAADSLVTAREVIRDSARIYKLFSTFMPKPFSNYPSSSMDVYIKLASNDGKDIMSNINDPKGLGLSEIAYNFLAGIIEHIGSIMAFASPTINSYKRFRETVTPSLLGLGTERHFIIRMPSNFRDSKSVEFRLADPLANSYLLLSAIIYAGLDGIERKLDVEPNVELGSLPSNLEDALHKLDSDNYIKYSIGAELISSYIELKNREIESYNNYITDWEVKAYLKAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G4</accession>
    <name>F4B9G4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777025.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G4"/>
    <dbReference type="STRING" id="933801.Ahos_2239"/>
    <dbReference type="GeneID" id="10601745"/>
    <dbReference type="KEGG" id="aho:Ahos_2239"/>
    <dbReference type="eggNOG" id="arCOG04081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028311_1_1_2"/>
    <dbReference type="OrthoDB" id="45425at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="63"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="449" mass="49827" checksum="3B70AE9AF28CA3F2" modified="2011-06-28" version="1">MVEIKEIKKVPEIEKASIHSHITGLGLDEHGKAIFKADGMVGQEEAREAAGLVVQLVKQGKMAGKGILLVGPSGTGKTALAVAIAKELGEDTPFTAINASELYSTELKKTEVLMQAIRKSIGVRVREKRLVYEGEVKELRIKVAKSRLNPYVQVPREAEITLKTKDDEMKLTAGESIAEQLVRLGVRKGDVIWIDAETGQVVKTGRSKEVAKYDIGGGGVEVPSGPVKKEKELTSTFTLHDLDLNLAARQISFTAIFSLFSEREISDDIRKEVDRLVKDMINKGNAELIPGVLFIDDAHMLDIEAFSFLTKALESELSPILILATNRGMTKIRGTDIESPHGIPLDLLDRLLIIPTKPYNEKEIREIISIRAEELNIELDPQALDELAKIGSQESLRYAVQLLEPANLIANRNGRNVIKPEDIKEVYNYFADIKRSVNYVKEYENLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G8</accession>
    <name>F4B9G8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3834 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777029.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G8"/>
    <dbReference type="GeneID" id="10601749"/>
    <dbReference type="KEGG" id="aho:Ahos_2243"/>
    <dbReference type="eggNOG" id="arCOG03692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243094_0_0_2"/>
    <dbReference type="OrthoDB" id="56755at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024533">
        <property type="entry name" value="DUF3834"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12916">
        <property type="entry name" value="DUF3834"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="23172" checksum="DC627E7363EAED4B" modified="2011-06-28" version="1">MQVIAAPGPVSYPIIAASTKNKDIKIVFSKEGKADVILDSTVSLVKRGLRINYVTIKELLSVYPNVGKKIGVWRKGSAADVLIRALLTTKGVNAELMYADDMRKILEMFKNKEIDSAVVASAFGKGITFEESLGIPGNCGAHVISNEDEFVSAYTEGIDMFKSDPEGFANAVSSTLKVDKEFVINTMKNSVLKVEKLNDDKDFAELVKKFIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J0</accession>
    <name>F4B9J0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1955 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J0"/>
    <dbReference type="STRING" id="933801.Ahos_2265"/>
    <dbReference type="KEGG" id="aho:Ahos_2265"/>
    <dbReference type="eggNOG" id="arCOG05998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574956_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015288">
        <property type="entry name" value="DUF1955"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036321">
        <property type="entry name" value="ST1625"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09205">
        <property type="entry name" value="DUF1955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101424">
        <property type="entry name" value="Hypothetical protein ST1625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17502" checksum="DD6F762225515A3D" modified="2011-06-28" version="1">MTEAKERIIDGYVKQGIELIEKTVSSNNISQANWVICNIIDAAKCEYLVEVLDSIGKIFDISVCGNVKRVISCYAKVGKYSEFVDIAINSIVNRGKKDQLDKVLNDVGNNGEFLYKLSLAYEKLHDLKKAQELRKKACDSGIPEACENINQVSTSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L3</accession>
    <name>F4B9L3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fructose-6-phosphate aldolase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_013775845.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L3"/>
    <dbReference type="STRING" id="933801.Ahos_1045"/>
    <dbReference type="GeneID" id="10600519"/>
    <dbReference type="KEGG" id="aho:Ahos_1045"/>
    <dbReference type="eggNOG" id="arCOG06034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097886_1_0_2"/>
    <dbReference type="OrthoDB" id="42145at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021890">
        <property type="entry name" value="DUF3501"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12007">
        <property type="entry name" value="DUF3501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="187" mass="22072" checksum="EE96664FC7B3482D" modified="2011-06-28" version="1">MVSITIKDVLPWREYEKIRMNEIRRISEIKKSRRIELGDRLSLLFENKDTVLHQIQEMIYLDRLEKEKDIEEIIRIYSTLLPGNGKIKATLYIYAYSMDDLVNVYKTLNKVYNSVFLKVVSKFIQGEPEAGREQGESFSTVQYLTFDLQGERSTNMEIHVLHENYKVTAKIPEELAKKLLKEVYDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Q4</accession>
    <name>F4B9Q4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1086</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775886.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Q4"/>
    <dbReference type="STRING" id="933801.Ahos_1086"/>
    <dbReference type="GeneID" id="10600560"/>
    <dbReference type="KEGG" id="aho:Ahos_1086"/>
    <dbReference type="eggNOG" id="arCOG03652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_586107_0_0_2"/>
    <dbReference type="OrthoDB" id="36693at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050367">
        <property type="entry name" value="APC_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770">
        <property type="entry name" value="AMINO ACID TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42770:SF11">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="17"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="467" mass="49745" checksum="D0748A11045BA995" modified="2011-06-28" version="1">MRLSKGSISLKEAYGQAMAVTAPLGSVVSTSTAAILYAGYSVVFTTLLALLGSALWIFTLTRYTSKLASAGGFYTYGYSAWRSKKISFFEALIEAFAYSFLNAVNAITAYLIISISLGIFGISLTPIEEGIIIFLAILYPTIISLSHIKFVLGKIVSISATAEAVLLIVMFAFSLTKGFHFNYMTPGNVPAHDLATAFVLSMVSISGAGAATYLGEEVKHPTKTVTLGMWLSLLIGGTAMFLGTYALVALWNGPLSALACSPQPLIYEMYSFGVLPLLIALVMSINSLFASNIGTTLGAARIIFNLSREKSAPAIFSKVNKSHEPVIATIFVGSITGLVTALSVIFLGINIAFADISAITGILWLSGRIIDGFGVPVFYYRIGQLGLVSTIIPLIATGLNLWGVTESISVPDIASIVILTSTMAVLIGWYLIKGRKGNPGSLVVDDNNEVIPIDEYLKKLKEKSVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9T4</accession>
    <name>F4B9T4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="8">FGAR amidotransferase I</shortName>
            <shortName evidence="8">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutaminase PurQ</fullName>
            <ecNumber evidence="8">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purQ</name>
        <name evidence="9" type="ordered locus">Ahos_1116</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EC" id="3.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9T4"/>
    <dbReference type="STRING" id="933801.Ahos_1116"/>
    <dbReference type="KEGG" id="aho:Ahos_1116"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002957">
        <property type="entry name" value="PRK03619.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="229" mass="25162" checksum="105F77A4B6E01322" modified="2011-06-28" version="1">MLGQRKLKTAVIKFPGTTCEIDVLKALKEAGVESNIVGYKDFDPDKFDAVVIPGGFSFGDYLRAGSIAANTETMKKIKEMANEGKIVLGICNGFQILVESGLLKGALLPNLKMRFISKWVYVKVVRFDTAITKGLNKKVLRMPIAHAEGRYYLDNPEEAEKLAVFKYSDEEGDVKEEVNPNGSILNIAGIANEEGNVIGLMPHPERASFKLTSPDSQTDGLLLLRGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9V6</accession>
    <name>F4B9V6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BPS2 like protein (Bps2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2301</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777087.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9V6"/>
    <dbReference type="STRING" id="933801.Ahos_2301"/>
    <dbReference type="GeneID" id="10601809"/>
    <dbReference type="KEGG" id="aho:Ahos_2301"/>
    <dbReference type="eggNOG" id="arCOG00373">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_468999_0_0_2"/>
    <dbReference type="OrthoDB" id="36891at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045487">
        <property type="entry name" value="ASRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc-hook" evidence="8">
        <location>
            <begin position="243"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="123"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="322"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE95172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="582" mass="68194" checksum="F4B6F675BBB4A6DF" modified="2011-06-28" version="1">MKVRVYNIGGIVSPLEVELTKGVNIYKAPNAYGKTSLAKALVSLLTSAIKPDDLLNVFANSGYVELDLDGRTYYRRIKRVKNKIMESSKLLLDDDRALLLSYFSPENKLLNQIMSGEENVEWFISATSKINELKSKKEEMETKLHNLQAEIDDLNRKHKEAIELQTKIKQIEDEIARLEKEKESDKVLNKTTQTISITNENKLRDIREKIEVKKRELEDLQNRIARLDQEIKNKESLASPEIKQSYERQLQEINTQLQKITSQRNEAEIEIRLLEKVLDQIRESEKQHLTTCYVCGSHVDPSIWKVRIDVISKELQEKNSLYSGIKKEADELLSKKSEIEKKLKELDQISSEISKLKMSRSELENRIESVKSTIDDLERQRREMEERFNRNAELYRVYDINDSINKRIEELKKKKDEYEYELAINGVPSTILNKISEKQKELQEVQKIVDDLEKEYMRRLTIAREEFVKIANYLLKELEFDLRAEIDENDRLVVKRNDATLELRKLSSSERTTLALILVLTALKSYFKTPFFIVDESFMTFDQRRFDKIVKYLNSITDYVIITKSDENIELLKETMEPLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y3</accession>
    <name>F4B9Y3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein Ahos_2328</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777114.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y3"/>
    <dbReference type="STRING" id="933801.Ahos_2328"/>
    <dbReference type="GeneID" id="10601836"/>
    <dbReference type="KEGG" id="aho:Ahos_2328"/>
    <dbReference type="eggNOG" id="arCOG04272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053134_0_0_2"/>
    <dbReference type="OrthoDB" id="9136at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00303">
        <property type="entry name" value="nicotinate mononucleotide-dependent phosphoribosyltransferase CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003368">
        <property type="entry name" value="PRK04447.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003372">
        <property type="entry name" value="PRK04447.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="346" mass="36882" checksum="004E82DF185B8C00" modified="2011-06-28" version="1">MIKEVLGNFIENLKTAKNYPFILVIATTDVSLIPGITVAGASPELTHFTPAADAEFLVLGKCKVINTVPITPDGIPTPALVTRASLSFTRVTKLVVNAGSRILPKIPFIDVGGKPGGDIRKYSLDLETTERIIENSTLLGEELSNTFDLLVIGESIPAGTTTAMAVLQGLGYDAIDKVSSASPNNPKELKRKVVLDALKNLPSNTLEKISKLADPMLISVAGISLGFKGKILLAGGTQMTAAAAIIKEFNKEKLKDIAIGTTKWIVKDKCSDIISLAKQVGVALIYADLDFSTSKHKGIRVYEEGYVKEGVGAGGASILAMINGAKNEDIVRRVDEMYSELLISKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z3</accession>
    <name>F4B9Z3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chlorite dismutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777124.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z3"/>
    <dbReference type="STRING" id="933801.Ahos_2338"/>
    <dbReference type="GeneID" id="10601846"/>
    <dbReference type="KEGG" id="aho:Ahos_2338"/>
    <dbReference type="eggNOG" id="arCOG03031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076582_0_0_2"/>
    <dbReference type="OrthoDB" id="8690at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1030">
        <property type="entry name" value="Apc35880, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010644">
        <property type="entry name" value="ChdC/CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843:SF1">
        <property type="entry name" value="COPROHEME DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36843">
        <property type="entry name" value="HEME-DEPENDENT PEROXIDASE YWFI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06778">
        <property type="entry name" value="Chlor_dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="220" mass="25868" checksum="E23FDE3D05E222E1" modified="2011-06-28" version="1">MANEVYMMVYSIKFDKDWWRLNHEARKTILSKAEEVANEFKKDVISIKKFSSLSQESNIIYWLTSFSTSPFLEFRSAILSAFEGYALESNLFLSVFKPSPYMKSNFDPKTILGGEQLKYFVAYPMKKSPEWYLLPFEEREKIMKEHIDMAIKNPKNNGIKSYTTYSFGIGDYEFVVIYEIPDLFNWVDVVEKLREAKARKWIVKEEPLIVGETKAFDFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA09</accession>
    <name>F4BA09_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dihydrolipoamide dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1122</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775922.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA09"/>
    <dbReference type="STRING" id="933801.Ahos_1122"/>
    <dbReference type="GeneID" id="10600596"/>
    <dbReference type="KEGG" id="aho:Ahos_1122"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="3"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="5">
        <location>
            <begin position="334"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="449" mass="48506" checksum="6816AF8787C184D7" modified="2011-06-28" version="1">MDFDVIVIGGGVGGYSAALRAAELGKKVAIIEKDEIGGECINRACIPSKTLIDAVKILNKIKKASWIKASATLDYEELSRFKDKIIESVKGKMIKNLENRSVTIIKGKGEIKREGEVDVNGKIYTTDKIVIATGSSPISLSAFPLNGKNVLDPWTAMNLPSLPQKIVIVGGGVAGVELATLFKAMGKDVTILELMPQLLPGFDKDIANETKKRLEERGVKIYLQANSKIIESEDKVVFDVTLPSSNEKVSGDLAVITIGRKPNTEGIDLQAVHVNVDKRGYIIVNDRAETSNPKYYAVGDVAGMPLSATKAWKQGIVAGDNIGGISSTMPKYSPISIFADLEIGSVGKTLDDLKKDNVEGKEITVKMEDIPRAWTLNETEGFLKIVVSGNKIVGAHMVGEGATEVINTLSLALEAGLTVDDLYKVLFSHPTVTEIISEVMQRLKYGEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA51</accession>
    <name>F4BA51_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775964.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA51"/>
    <dbReference type="STRING" id="933801.Ahos_1165"/>
    <dbReference type="GeneID" id="10600639"/>
    <dbReference type="KEGG" id="aho:Ahos_1165"/>
    <dbReference type="eggNOG" id="arCOG05991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177744_0_0_2"/>
    <dbReference type="OrthoDB" id="33090at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13710">
        <property type="entry name" value="ACT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="109" mass="12637" checksum="FD1C9EAB07B2C931" modified="2011-06-28" version="1">MTQGRIVKVTGYYRDPGFLERVISTFRKLWVDIDWVTARRISDDGLYEVYLLVRETKNTHLAILNLSKTVDVEKVEVLEDGKLIPCQNDSFFIPSYTKVTTYSWGEKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA66</accession>
    <name>F4BA66_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="9">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">metG</name>
        <name evidence="13" type="ordered locus">Ahos_1180</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775979.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA66"/>
    <dbReference type="STRING" id="933801.Ahos_1180"/>
    <dbReference type="GeneID" id="10600654"/>
    <dbReference type="KEGG" id="aho:Ahos_1180"/>
    <dbReference type="eggNOG" id="arCOG00810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_2"/>
    <dbReference type="OrthoDB" id="371856at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="11">
        <location>
            <begin position="3"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="12">
        <location>
            <begin position="433"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="333"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="572" mass="66641" checksum="51F30F925100BCAB" modified="2011-06-28" version="1">MKIYVASAWPYVEAVPHLGNLIGSVLSADVFARYARLKYGKDNVIFVSGSDEHGTPIEVEAIKRKINPRDLTNQAHEYDKKLFLDVWKISYDNYTRTESETHKRFVREFLLSIQDYIKVQEEEMPYCEHDKIFLPDRFIKGTCPYCGYEDARGDQCDRCGRLLSPTLLINPRCAICGSKPVIKKTKHWFFDLSQFSDKLKEWLENNKDMPDNVKSVALSWIQEGLKPRSLTRDTSWGIPAPFEGAEGKTVYVWFEALLGYISATIEYFEKTGNPDEWKEFWFGDDVKSYYFIGKDNIPFHAVIFPAMLMASKKGYKLPSVISSTEYLMYEGQKFSKSRRIGIWIDEAPRLMDVDYWRYVLIRLRPEEKDTNFLWREAIRIINTELNDDIGNYVNRVITMVNRYFKGYVPNFNEDKLDDIDRKFMELVKTTPVVVSQLFEKGKLKAGSDEIIKIAREGNAYLNQKAPWDKVKNDIKVAENTLYIASNSVRTLSILLYPIIPTSAEKIYNQLGLRGIENEKWDSAGEFILDHNHKVEQASPIFKKIDQNFEKELSDKLEKIRKELDNIRPPLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA68</accession>
    <name>F4BA68_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaetidylinositol phosphate synthase</fullName>
            <shortName evidence="2">AIP synthase</shortName>
            <ecNumber evidence="2">2.7.8.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of archaetidylinositol phosphate (AIP) from CDP-archaeol (CDP-ArOH or CDP-2,3-bis-(O-phytanyl)-sn-glycerol) and 1L-myo-inositol 1-phosphate (IP or 1D-myo-inositol 3-phosphate). AIP is a precursor of archaetidyl-myo-inositol (AI), an ether-type inositol phospholipid ubiquitously distributed in archaea membranes and essential for glycolipid biosynthesis in archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1D-myo-inositol 3-phosphate = saturated 1-archaetidyl-1D-myo-inositol 3-phosphate + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36823"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74004"/>
            <dbReference type="ChEBI" id="CHEBI:74006"/>
            <dbReference type="EC" id="2.7.8.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.39" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775981.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA68"/>
    <dbReference type="STRING" id="933801.Ahos_1182"/>
    <dbReference type="GeneID" id="10600656"/>
    <dbReference type="KEGG" id="aho:Ahos_1182"/>
    <dbReference type="eggNOG" id="arCOG00670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080384_1_2_2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02242">
        <property type="entry name" value="AIP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044270">
        <property type="entry name" value="AIP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054868">
        <property type="entry name" value="archin_ph_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040950">
        <property type="entry name" value="archin_ph_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="191" mass="21296" checksum="4BD227BB9B2FD7E2" modified="2011-06-28" version="1">MITRLRRESKRILRPIANALAKIGVKANEITIIGLVLSIIYFIVLYISKNPLYGVILLALSSFSDALDGEVARISGTAGNKGAFLDSSLDRVEDTLFLSPLVLYFQPVLVSLLVGISLIIPYLRARAEALGIKAEGRGIIERGERIIFTMIILILLFFNFTFTIYVFYLFIALSIVTVIQRFQLVLSNLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA70</accession>
    <name>F4BA70_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1184</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94067.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="18124" checksum="0A1EC0B03CBA725C" modified="2011-06-28" version="1">MTYTPHVPYVPTPEPVVRKMLEIAKVGPEDIVYDLGCGDGRIVITAAKEFNAKKAVGIDINDERIKEALENVKKNGVEGKVIIEKGNFFDVDLSEATVVTMFLLTNVNEMLKPKLEKELKPGTRVVSHEFEMRGWTPKEVVKVEDGNMNHIVYLYVIGEHK</sequence>
</entry>
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    <name>F4B3Q2_ACIHW</name>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q2"/>
    <dbReference type="STRING" id="933801.Ahos_1206"/>
    <dbReference type="GeneID" id="10600682"/>
    <dbReference type="KEGG" id="aho:Ahos_1206"/>
    <dbReference type="eggNOG" id="arCOG04235">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095574_0_0_2"/>
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        <property type="component" value="Chromosome"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="74"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="260" mass="30411" checksum="A6756FF9A48BE569" modified="2011-06-28" version="1">MRVEIFTHKNCTECNLLLEYLDQKGLLGKVQIIDTELYPFLALERGVISTPSVFIDGKLVYAGTVDFQEFEKILQGEKIVKKINKDELVDKLMYGIVDSFAATAWLYVNLDFDSFMAQKDFVMAVTGLVFSEDAEEQYNYLRNLMIKNGLEYVKKWEDKMLRNISSNFVREIYWLYGSKLSLEQIKAKYPLEVFAHWLMVRGGSTGRVGLRIHPLTEKDTMERISKAYMYMINNYDQLWEKVEKEQKSLKSIEVERKAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V6</accession>
    <name>F4B3V6_ACIHW</name>
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        <submittedName>
            <fullName evidence="5">ATP-cobalamin adenosyltransferase</fullName>
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    <gene>
        <name evidence="5" type="ordered locus">Ahos_1260</name>
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    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V6"/>
    <dbReference type="STRING" id="933801.Ahos_1260"/>
    <dbReference type="KEGG" id="aho:Ahos_1260"/>
    <dbReference type="eggNOG" id="arCOG00489">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
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        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
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        <location>
            <begin position="8"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="177" mass="19985" checksum="607EACC35FE95EA2" modified="2011-06-28" version="1">MVFNHMWYTGSGDSGKTKVPSKGEVWKDDDVVEALGNLDELNASLGIISSLYPQLFDLIYKIQDDVFVISSEIAGFNLNFSEEKIKELEDLIAKFGGEIKPLRNFVLPGGHLASSFLHLSRAICRRAERSVVKIMREGKARDVHVKYLNRLSSLLFVLALWINEKSGNPNVIWKRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V8</accession>
    <name>F4B3V8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Ahos_1262</name>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776061.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V8"/>
    <dbReference type="STRING" id="933801.Ahos_1262"/>
    <dbReference type="GeneID" id="10600743"/>
    <dbReference type="KEGG" id="aho:Ahos_1262"/>
    <dbReference type="eggNOG" id="arCOG07265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136101_0_0_2"/>
    <dbReference type="OrthoDB" id="43181at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="255" mass="28487" checksum="EA3C20A5E6B3D9F6" modified="2011-06-28" version="1">MKIETMDIEDKRSNTIIAWAKDPSNIISVLPNVVGIENNVVKLKFTRFLLFSFEGEFVVEPSFIGSGIIEYKLTDKKRNEIKIIISTEGKSGVKLSVSYSGEKEWIVGKGLHKILKEISNGIKSELSKLPEVEQQTVGSKNFSLSLSKISSISKLIMKSKLAKTEDVTLKEGEVLSYVEGIISDFANYPLIYISGSGSSTFRLLFLNGELKGIYILKDGKDSFNEDDLNHISGEFKIHVYVGISPRIVEVLEQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W4</accession>
    <name>F4B3W4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="2">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="2">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1268</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W4"/>
    <dbReference type="STRING" id="933801.Ahos_1268"/>
    <dbReference type="KEGG" id="aho:Ahos_1268"/>
    <dbReference type="eggNOG" id="arCOG01026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061764_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043793">
        <property type="term" value="F:beta-ribofuranosylaminobenzene 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="BETA-RIBOFURANOSYLPHENOL 5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="3">
        <location>
            <begin position="193"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="285" mass="31814" checksum="F3CA91BF76BFB378" modified="2011-06-28" version="1">MKLIGISRIHITLFDLEGKYGRLDGGMGVALKYPRIVIKTGECEEINIDGVPKEKFCIEEDYPAHVGLGHTTQYLLSITKYAFEKNFQRKTSVELAKLVKRGSTSGIGVYAFEYGGFIVDGGHSLKVKKEALPSDFSTAPPPPLLLRVDFPWYIYVNIPEGRRIFGKEELAAFKNAKLEGLDTLARVVLMEFIPSVIEKDLENVLDALDRIQNLGFKKIEVSLQTDKVRELMKKLKSKGFPSGISSFGPAVYTFVNSRREGEELVSTFGGFLTEPNNKGAKVVWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3X8</accession>
    <name>F4B3X8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ferritin, CCC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0047</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774859.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3X8"/>
    <dbReference type="STRING" id="933801.Ahos_0047"/>
    <dbReference type="GeneID" id="10599487"/>
    <dbReference type="KEGG" id="aho:Ahos_0047"/>
    <dbReference type="eggNOG" id="arCOG01096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065373_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01044">
        <property type="entry name" value="Ferritin_CCC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039376">
        <property type="entry name" value="Ferritin_CCC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Rubrerythrin diiron-binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE92942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="320" mass="34920" checksum="46468CF69C99D27B" modified="2011-06-28" version="1">MISELIRKNYKEELFGATVYEELAKTEKNVKVKEVLEELANGERSHASFWKEVAESRGVELEELGFFDRLKIKILKLFRRVFGLPLTLKLVERGEISDAEKYYQLSRAAEFNDTERQKLSSIMMQELVHEDLLVQTQINADKIRDAIYAVSDGLIEVLAGVSGLSSILASPFLVALGGIIIGVSGTISMSIGAYLSSSSENDIRKSKLKKEKLRTLLGQAYSSEGNNEINKNSESVKITAISYILGALVPILPFLLGLGGLLGLITSYAFTGVVTFIVGGIIGILSDVNPFKKGAVMAGLAIVAALVTHLIGIAFHFIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B401</accession>
    <name>F4B401_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B401"/>
    <dbReference type="STRING" id="933801.Ahos_0072"/>
    <dbReference type="KEGG" id="aho:Ahos_0072"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00838">
        <property type="entry name" value="MPP_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="300" mass="34549" checksum="F3BC26161C582B89" modified="2011-06-28" version="1">MFVTDLHGSEIAYRKALNAAKMHNVDYLIIGGDITSKNIVFIERSGDKYYFNGNEVDIKTVYEDARKYGYYIYVASKDEIQKILENKEFFESVKRTLIEKQIDNWIKIAEEKLTGTNIKVFWSYGNDDPLYLDEVFSKYNIRDEGIFELEEGKTNTLYLISYGYVNPTPFKSYREEPDSTIYIKGEDYLRKIDEPERAILNFHAPPYNTKLDIAIIDGKKREHVGSRGVRDLIEKYRPLLGLHGHIIESPATDKIKNTIVVNPGSLAYEGLLKYSIIVIERKLEGLLVKYKIKGAAILSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B407</accession>
    <name>F4B407_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0078</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B407"/>
    <dbReference type="STRING" id="933801.Ahos_0078"/>
    <dbReference type="GeneID" id="10599518"/>
    <dbReference type="KEGG" id="aho:Ahos_0078"/>
    <dbReference type="eggNOG" id="arCOG02683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_14_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="7"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE92971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="455" mass="49834" checksum="A010052D2C010548" modified="2011-06-28" version="1">MRLEWRNVLVSGMGVFTDGYNLYSISLAYYFISSSFHFVNLTLGLIIASSYYGAALSALLFGIIADKQGRKIMYGIDTALMTIGALLQAFSKNIPELFVSRLILGMGIGADYVLSPIIVAENARAENRGKLMVITFAFMWGLGAVAAAFVEQMLILLHLPSYIIWRIVLGFGAVPAFSVIFLRRRIYETILFVSRVKPLEKDIKGIEKELGKKLTIVKDTTPFIKRLKSSTLLIIASSILWMLYDMYSSTFSVYGPIVIAGNLGISAIEFTYIAQFAAGIPGQLICMSTIDKVGRKPLIVIGYAGVSFWLMMYFLLLYDPSLFGLHILVPPNPLYAAKSLVGEAALLGFTFYLLNYLFSAIGPASIIGSAMVTPEIVPTKIRGTGQAISVSADRLSAALVSTAFPLLLAKYGLATMIGIYSLIALISSIITLIFIPETKKKELEVFEKPISSQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B423</accession>
    <name>F4B423_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane protein 6-pyruvoyl-tetrahydropterin synthase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774904.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B423"/>
    <dbReference type="STRING" id="933801.Ahos_0094"/>
    <dbReference type="GeneID" id="10599534"/>
    <dbReference type="KEGG" id="aho:Ahos_0094"/>
    <dbReference type="eggNOG" id="arCOG08510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491445_0_0_2"/>
    <dbReference type="OrthoDB" id="42055at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="521"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="64450" checksum="43818B7175F2D8CA" modified="2011-06-28" version="1">MRRFYLFFVVTLAFYLIVFRTLFEVNAFSLLDTEPSYLYYRQYLFSIPTPFSYFYNFLNSIWWNSPEKIFTVLPFILGTVLFYVMSSYFNLIEWKKWILSFLFMLNPGTALILYVGDAPSILMSYAFGPLVFLLGYRLAKNINLKNFLILSLVFIVTNWIYFESFILDFIFLLPILFFASNKIKFLLASLASSALSFLSLVNYEVLVYEIVVPSLNITSAHYIPLYVKEIYYISFYLGIFSIYLFIKKYKIPSAIVSVSLILLLSWLVVFVKIPNIPLINAIFLIFQSFQQKFILFISGLVVISFAFLKSWKYIFFILFLMSLANMPCINLYTQEHVTAYAILTFNANNYAVCEEFFKLQKFIYEHPGFYYIGINKTFLLECNLYSIYKYENLIPNLLPVSFSIFGVNAKQLAYEGIKYYLVVSNETPNYSNFKLVFSCGKFHLYENECFKSIAFSANGEPLNVTVTPCKVIVIGNSTFAKVLVPYYGLWKGAKDCNGYLGIPMNNGYGEAINEAYYINQYLIIVDFIVLIIISGLLAKLCWSKLRSVIWLKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B432</accession>
    <name>F4B432_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Archaeal hydrophobe/amphiphile efflux-3 (HAE3) family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0103</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774913.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B432"/>
    <dbReference type="STRING" id="933801.Ahos_0103"/>
    <dbReference type="GeneID" id="10599543"/>
    <dbReference type="KEGG" id="aho:Ahos_0103"/>
    <dbReference type="eggNOG" id="arCOG02175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_276309_0_0_2"/>
    <dbReference type="OrthoDB" id="42357at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
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        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF6">
        <property type="entry name" value="MEMBRANE PROTEIN YDGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="616"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="648"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="721"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="754"/>
            <end position="775"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1005"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1011"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1072"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1099"/>
            <end position="1125"/>
        </location>
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        <location>
            <begin position="646"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="994"/>
            <end position="1123"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE92996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="1136" mass="128034" checksum="13218B5BBCE69CF4" modified="2011-06-28" version="1">MNSRFSEFISKKWYVVLIIWIIVLAISAPFSTLFFKSVSYQINISTPGSTSAQAENIVSKYFHLQGASGANAILIIEGNVTPYSLFLANLTSYGNITVISYYSLEKNLLNTTLSKLQNSTNSLYVKILEIKSGEIKAENNLSCKYIELNNTIVKLEELNYVTKKVEVAFINVSNEINETTIRLYQLHQGMLNNLSTFCKIKEGEMEINSTAHNASLFLYGYTSLFLKNFLEIYNETHNFTYSSEKAFEEVSKLINNVNESKFFYTFYKFWLESDCTNFVQRAQISIENASMLLPNNSFILYLENYINISNFEEGLPYYKFTVNYINETKHVPLELAEELYYNQPKDVLLNIYSEKTGLNETFLQEVLNQTNFKELAYDLILSKANVTEKPFISAVYYNLNKSPYEFAVDYVSSQYNVSPTLVNEISNFTEYSQYVNFVSLQASNESKLPQWFFQQLLYYHNISNLTAYLISPKLSKLQPILNASNITPKEFLIYLQTANNESVYNLTAKLISNYVNFTPIICVNRSLLQEVLSRNESVSSLVSELIRDNEFPVTPITNITDALYYKNYYLIIMKGNFTINEAKDFQNYVKSIIPLTSYLTGPKPVDQQLRGLANSAFAIAIPVGIALAIIITGIYFRSFVAAFVPLGIYLSAFLVSSVLIWLIVIKLLGITVDFLTPSQVLLLALGLGTDYVVFISSRYIEERRNGLSKDEAVKEAVRWGGRAVTLTALVVMLSFLFLYIYNVPLVSDTSISEMLAVFVVWLSATTLFTSILKAAGDKLFFPAKFNRDNNTKSYSVSRPATKVGIFITLVVIFSIIALSMPLSTNILGLMPPSQASEGVNLLSQEFTTSNVFPIYVVVPISTFNQTTYNYAVHIYKELSSIPGVTAVESPVSPYGGLIPYCKLSEYNYTEYISHGYMLFVVNQKYPPFSTQAFGVVENIESLHIGYVGGGPVDLYNILHFIETDFLEIFLLITVTMYVLLVIMTRSFSVSGVVIFTIMSAVGITLGLERLIFVNILGYGIFAVVPLFLVAIIIGIGMDYNIFLIARIHEEIEKEENMETAVGKSVKAIGKTIIFLGLIFAGTMGSLMLVKAAILQEIGFALSVAAILETSLLWYYLAPSLLVLLYRKFKIRPKMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B439</accession>
    <name>F4B439_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774920.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B439"/>
    <dbReference type="GeneID" id="10599550"/>
    <dbReference type="KEGG" id="aho:Ahos_0110"/>
    <dbReference type="HOGENOM" id="CLU_1313061_0_0_2"/>
    <dbReference type="OrthoDB" id="44009at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="209" mass="23727" checksum="EA9272F2687413B2" modified="2011-06-28" version="1">MKSRTVKLRMIISLTLVVIGFTLAFIFLPYLNMTNNSSTSGIKMVNSQIVDKYFGGKWNENDSLSGYVKPQKSYYEIHFYNGSTENVSSENLTIVNPQINKYLGFIEVDLRSINFTTFYSGNQTLSIIILNYTSTMEPKNIYNTIYTTFKDNSKAVALSFHNISNCAFIASIDCYQFSIGYSSSEIIIVFYKGCIDESLAITDLINYML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>F4B451</accession>
    <name>F4B451_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="11">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">moaA</name>
        <name evidence="13" type="ordered locus">Ahos_1276</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="11"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776075.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B451"/>
    <dbReference type="STRING" id="933801.Ahos_1276"/>
    <dbReference type="GeneID" id="10600757"/>
    <dbReference type="KEGG" id="aho:Ahos_1276"/>
    <dbReference type="eggNOG" id="arCOG00930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="OrthoDB" id="6925at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="4"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="250"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE94159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="308" mass="34867" checksum="967C848483F85C27" modified="2011-06-28" version="1">MKDRFGRDLEDLRITLTHACNFSCFFCHMEGEGNENLSGLPLRDILLVAKASKDFGIKYVKLTGGEPTLRRDLIEIVKGLKSLGFEVSMTTNGVLLPRLATKLKEAGLSRVNISLHALDRETFKKITGVDALDKVLEGIKSAINANLRPVKLNFVVNKMNINEIKEIIDFSEKLGIDELHLIEMHPVGLGKSSFSYHENLNIIEDYLKKNSTKVTIRNKHFRPRYYLNSGLVVEVVKPYANPIFCAGCNRIRLTVDGKLKTCLYREDKVIDISGILKTNLPEDDKLELIKESFKLAIAIREPNFKYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A3</accession>
    <name>F4B4A3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable glycine dehydrogenase (decarboxylating) subunit 2</fullName>
            <ecNumber evidence="5">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glycine cleavage system P-protein subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycine decarboxylase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycine dehydrogenase (aminomethyl-transferring) subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gcvPB</name>
        <name evidence="8" type="ordered locus">Ahos_1328</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GcvP family. C-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776126.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A3"/>
    <dbReference type="STRING" id="933801.Ahos_1328"/>
    <dbReference type="GeneID" id="10600814"/>
    <dbReference type="KEGG" id="aho:Ahos_1328"/>
    <dbReference type="eggNOG" id="arCOG00076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_5_0_2"/>
    <dbReference type="OrthoDB" id="371967at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000224">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000014">
        <property type="entry name" value="Probable glycine dehydrogenase (decarboxylating) subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00713">
        <property type="entry name" value="GcvPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023012">
        <property type="entry name" value="GcvPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003346">
        <property type="entry name" value="PRK04366.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF1">
        <property type="entry name" value="GLYCINE DEHYDROGENASE (DECARBOXYLATING), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="6">
        <location>
            <begin position="35"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="352"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="502" mass="56014" checksum="6A4B27D4F3150CBD" modified="2011-06-28" version="1">MWRQANWDEPLIYELSSDKKRKQGIIIPEENIDVKINIPEKIKRKNDLEIPELSELEVVRHFVRLSQQNFSVDLGMMPLGSCTMKYNPKVEELTSRVVENYHPLQDEDTVQGILEMMYEMQKWLAEMTGMDECSLQIPAGAAGELAGVLMIKKYHETKNRKRDEMLVADTAHGTNPASAAMAGYKVIYIKSNQEGLVDIDILKEVVNSKTAGFMLTNPNTLGLFEENILEISKIIHSVDGVLYYDGANLNGILGIARPGDMGFDIVHINLHKTFAVPHGGGGPGAGAICAKGELKDYLPYPIVDKEGDKYVLRTPVKTIGKIASFYGNIGNVIRAYVYILGLGPQGISQVGKMSTLATNYLISKLKDVKGLELLAPHRPRKHEVVFSAKPLLNDKGVTALDIAKALLDRGFYAPTIYFPGIVEEALMIEPTETEPKETLDKFIAALKEILEESYKDPEKIRQTPQNTSVKRLDQVKANHPTSVTPSYRVLRLREKGIIKILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A6</accession>
    <name>F4B4A6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enolase</fullName>
            <ecNumber evidence="6">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">eno</name>
        <name evidence="12" type="ordered locus">Ahos_1331</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds a second Mg(2+) ion via substrate during catalysis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell surface</location>
        </subcellularLocation>
        <text evidence="6">Fractions of enolase are present in both the cytoplasm and on the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776129.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A6"/>
    <dbReference type="STRING" id="933801.Ahos_1331"/>
    <dbReference type="GeneID" id="10600817"/>
    <dbReference type="KEGG" id="aho:Ahos_1331"/>
    <dbReference type="eggNOG" id="arCOG01169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_2"/>
    <dbReference type="OrthoDB" id="8680at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="11">
        <location>
            <begin position="7"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="10">
        <location>
            <begin position="136"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6 7">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="360"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE94214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="417" mass="46301" checksum="D05D9BE708DBE0CB" modified="2011-06-28" version="1">MSDQFIIKKIKGIEILDSRGNRTIRVFVETEGGIKSFGDAPAGASKGSREAIELRDSKGGVKPAIDSVNYFINDALRGFDVRRQEDIDRTLIQLDGTDNKSKLGANAIIATSIAVAKTAAKALGLEIFQYIGGGRKHRIPVPLLNILNGGLHAGNNLKIQEFIIIPLKFNKFSEALFASTEVYKQLKNLVTEKFGKIYTALGDEGGVSPPLNKTEEALELLSDAVKKAGYEGEFFFGMDAASSDFYNEKEGVYEIDGAKKSAEEMIDYYSELAEKYPVLYLEDPFNENDFEHFAELQSKLKNTIITGDDLYTTNVKYLKKGIENKSTRGVIVKPNQIGTLTETYEFFDTARENSIKTVISHRSGETEDNFIADLAVGLNSDFIKTGAPARGERTAKYNRILEIENEYEIEYGNKNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4A9</accession>
    <name>F4B4A9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cell division protein CdvB, Vps2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776132.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4A9"/>
    <dbReference type="STRING" id="933801.Ahos_1334"/>
    <dbReference type="GeneID" id="10600820"/>
    <dbReference type="KEGG" id="aho:Ahos_1334"/>
    <dbReference type="eggNOG" id="arCOG00453">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1072074_0_0_2"/>
    <dbReference type="OrthoDB" id="36371at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053654">
        <property type="entry name" value="Cell_Div_Complex_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041008">
        <property type="entry name" value="cell_div_CdvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="258" mass="29124" checksum="D0886FEC949D2A3F" modified="2011-06-28" version="1">MLEKLPIFSRFNGDKKRRAQLGKLLTEISIKLKDQQSKLEEGMHRLKERDKELFEKLVRAQIEGDTARATIYAQEISDIRKMIKIIYTAFLAIEKVRLKLDTVQELQGVSLVLFPVVKILGELKDQIKGIAPEVALALDSITSSVNSIAVETGALSDKTVLPSTIDEQAKKIMEEAQKTAEIKIKEMLPDLPHPPSTTQVKLQYEKRKLDEKELLNYINSNGGFLDVEYVVKKYGVEKDEVMGLLKEMANKGLISIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G2</accession>
    <name>F4B4G2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="6">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="6">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">panB</name>
        <name evidence="10" type="ordered locus">Ahos_0158</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6 9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774968.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G2"/>
    <dbReference type="STRING" id="933801.Ahos_0158"/>
    <dbReference type="GeneID" id="10599598"/>
    <dbReference type="KEGG" id="aho:Ahos_0158"/>
    <dbReference type="eggNOG" id="arCOG00584">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036645_1_0_2"/>
    <dbReference type="OrthoDB" id="8414at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <begin position="46"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000388-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="264" mass="29315" checksum="386DE9E151AF692E" modified="2011-06-28" version="1">MLEKITVKSFLKKKERKEKITMLTAYDYPTAKIISLTELDGILVGDSLAMVVLGLENTLKVGMKEMLHHLDAVVRAKPRQLIVADMPFLSYETGDALKNAGLFAKHGADAVKLEGGEEFSDVVKKIVRAGIPVMGHIGLTPQRFLRLGGYRTIGKTESEEEQLLRDAKALEEAGVFSVVIENVYSSIAKRITESLSIPTICIGAGPYCDGQILVIHDLLGLSDFKPYFAKAYIDLKEIISKAISEYVREVKEGKFPSKEYYKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4G3</accession>
    <name>F4B4G3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774969.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4G3"/>
    <dbReference type="GeneID" id="10599599"/>
    <dbReference type="KEGG" id="aho:Ahos_0159"/>
    <dbReference type="eggNOG" id="arCOG14120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154301_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="240" mass="26963" checksum="14106F09D45A977E" modified="2011-06-28" version="1">MKSKATLLALLILLLMPGIFYSDALYYYHGESQFCTSKLIKIYTFSYHLPNANVRSSVNSGNNVSIKICEYNPPPPPHSQKLINVTKICLPKSSKVFLIPIIYNISKNGPKLSYCCISLTFTSLPNTYKACLVTKAKPQNKTFYFVTECNYYFVNVCWTFCGPPPPTAWANILLMFIVQEGGANYVYFWSINITLSPAHPPPPPGPPPPPPPPSPYCYIVEYPNLCPLKVAQIIFSLYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H0</accession>
    <name>F4B4H0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Succinyl-diaminopimelate desuccinylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0166</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774976.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H0"/>
    <dbReference type="STRING" id="933801.Ahos_0166"/>
    <dbReference type="GeneID" id="10599606"/>
    <dbReference type="KEGG" id="aho:Ahos_0166"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1275247_0_0_2"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008777">
        <property type="term" value="F:acetylornithine deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF31">
        <property type="entry name" value="N-ACETYL-L-CITRULLINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="216" mass="23923" checksum="08F7D3387B69BB5E" modified="2011-06-28" version="1">MLIELTKKLISFKTYNDNELHECALFIKDYLENQGFKAEIKEYVKGYPVVISSSGKGKPLMLNGHFDVVPPGEGWSSDPFVPKVVDDKIYGRGATDMKAGLAVLMETYVLMADKLDYPLLFTAVSDEETGGKRGSKFLAEEFSPFFVIIGEPTGSERINIGEKGLLQIKIKERGKSAHGSTPSLGDNSILKLIDDILILEKEINDFSVSIPRKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H6</accession>
    <name>F4B4H6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774982.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H6"/>
    <dbReference type="STRING" id="933801.Ahos_0173"/>
    <dbReference type="GeneID" id="10599613"/>
    <dbReference type="KEGG" id="aho:Ahos_0173"/>
    <dbReference type="eggNOG" id="arCOG00749">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_10_0_2"/>
    <dbReference type="OrthoDB" id="312811at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729">
        <property type="entry name" value="OLIGO/DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42729:SF1">
        <property type="entry name" value="OLIGO_DIPEPTIDE TRANSPORT, PERMEASE PROTEIN (DPPC-2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="265"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="474" mass="52575" checksum="2800E828C6F22A2A" modified="2011-06-28" version="1">MNRTLKVLISQKSFIVSVVLIGSIVGLALYSLTFGNYNPYNPSSVVAGRFAVPAWATIFPQYKDLPPDISKYLTPSMPIKQYNSTSYDVIVAPNSEKVINFPIDWKYTTPKCGFEISFNLKPIVECSKQFFMINTTWTLPNGTSYDLATLMPSSYSIYYFSTYYCFIQVNKTFDMYYGTLRLICHSVYLCSLPIEQRTVYSVYLPLLMFPKEGLYNLSLIIINNSSKPLTIIFTRPHFSVIGETYGLLGTDDNGAPVFKEFAIGARLDLEIALFASVLIVLIGAVFGMISGYYGGKIDLSIVGLTDFFLLIPGLPLLIALETALIKSGIHMSKLLLIIILISILSWPGTSRIIRSQTLSIRNRTFIEASRALGLNNLRIIARHVVPNLIPIIAAQVAYDVPTVILIESGLDFLGLGILCYPTWGNMLGFASHTVSSANGFAWWWVLPPGIGIIVLSAAFFYLGNAFIKAFSPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H9</accession>
    <name>F4B4H9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774985.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H9"/>
    <dbReference type="STRING" id="933801.Ahos_0176"/>
    <dbReference type="GeneID" id="10599616"/>
    <dbReference type="KEGG" id="aho:Ahos_0176"/>
    <dbReference type="eggNOG" id="arCOG07232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567043_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="170" mass="19945" checksum="AEFCE7B7C3ED59D1" modified="2011-06-28" version="1">MSEEVSEIVSKSEKILSFFISVISIIISFYLGIFSYYLLILLFLSVSVFIFRYNLLIKLILSKNDKISIIPRPSLEKRRALQNLIIISSLIFSPFLLIYIFPSILWITFTIAIVTSWPFSAIIALLVIYILEKRRGVKIYKYVIFDERLDEINIKEYGIIAYRQDSLRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4J1</accession>
    <name>F4B4J1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfide dehydrogenase flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774997.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4J1"/>
    <dbReference type="STRING" id="933801.Ahos_0188"/>
    <dbReference type="GeneID" id="10599628"/>
    <dbReference type="KEGG" id="aho:Ahos_0188"/>
    <dbReference type="eggNOG" id="arCOG01065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_0_0_2"/>
    <dbReference type="OrthoDB" id="38899at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049386">
        <property type="entry name" value="FCSD_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052541">
        <property type="entry name" value="SQRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43755:SF1">
        <property type="entry name" value="FAD-DEPENDENT PYRIDINE NUCLEOTIDE-DISULPHIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21706">
        <property type="entry name" value="FCSD_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfide dehydrogenase [flavocytochrome c] flavoprotein chain central" evidence="2">
        <location>
            <begin position="140"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="391" mass="44063" checksum="CF9024C2D2B5173A" modified="2011-06-28" version="1">MKKIVIVGGGIAGMGIANTLADKLKGKAEITVVNKEDFYFAGPSRPLILTGEQNYHRIIRGYENVGVKGIKLVVGNVYKVDPDNRKVYVSESTFGNKDSVLDYDYLVLTPGIVFDGSKITGYEKYWWKNTTVYDPGRVNVLKERLWSQNEGTVIVYAPKAPYRCAPAPTETTLLSHTILTHRGVRQKFNIIHIDANDKTQPPFIMDLVKQTYDKSGIQLVTNQEITEIGEDYVVTSSGEKYKYTILAMLEPNRAPKFIDEAGLGKGYVDIRSPQDLRHPKYDDVLSAGDAAKLPFPKNQEIAFESAMFAANKILEMEGVSEKVSVQYAFVGWAYMGNLEGKLETLSLQFQMDLTTQPPKPTKDPQLKRDYTLQKDRWEQAYLEKLFGYKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N2</accession>
    <name>F4B4N2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="8">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argS</name>
        <name evidence="11" type="ordered locus">Ahos_1379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776177.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N2"/>
    <dbReference type="STRING" id="933801.Ahos_1379"/>
    <dbReference type="GeneID" id="10600866"/>
    <dbReference type="KEGG" id="aho:Ahos_1379"/>
    <dbReference type="eggNOG" id="arCOG00487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="OrthoDB" id="372102at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002446">
        <property type="entry name" value="PRK01611.3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="10">
        <location>
            <begin position="507"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="623" mass="71641" checksum="E907DF504B32C0EC" modified="2011-06-28" version="1">MYALAKAKDELANYLSNVLNVDKEKVLNSIEYPTKEQIADLALPLPSVTKKFDIEIKGEYNGRLIKEVWKDGLFINARLNEKELLKEIFTNFSENYGIIKTERPLRIVVEHTSANPIHPLHIGHLRNAILGDTLAKMLKARGHEVNVRFYVNDSGRQVALLIYGLSKLGYPDPPSEEKKDQWLGLIYAMTNVILEIKKINEELKSTTNDSEYKEKIARRDELLISASELSKRNQEYFNKLSDQIMNDKDPEGEISKIIEKYEKGEEDIKKIVRKYVDFALEGFKESLEKLHIMFDNFDYESDLLWNNDVKLVLDFALTSVAKINYKGTAALDLQNYIDDDIRKEIRIPKGLEIPPLVLMRSDGTTLYTVRDIAYTIYKFRQFNADEVINVIAEQQSVPQMQLRAALYILGWKKFAINLIHFSYGMVTLQGMKMSGRLGKYISLDEVYDKVAEVVKAKIEEKKGILENLNEIVNSAIRYAIISVSANKPVSFDVKRVANFEENSGPYLQYTYARAYNILSKSTENLDISKIDENELKNEKRTLLLMIAKFPDIFINSVDHLQPEVLTVFLRGLADVFNSWYDKERVLQEPDESKRITRLFIVKGVETVLRNGLNTLGIASLTRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4N8</accession>
    <name>F4B4N8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="5">Glu-ADT subunit D</shortName>
            <ecNumber evidence="5 7">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gatD</name>
        <name evidence="11" type="ordered locus">Ahos_1385</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 7">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="5 7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776183.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4N8"/>
    <dbReference type="STRING" id="933801.Ahos_1385"/>
    <dbReference type="GeneID" id="10600874"/>
    <dbReference type="KEGG" id="aho:Ahos_1385"/>
    <dbReference type="eggNOG" id="arCOG01924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_2_1_2"/>
    <dbReference type="OrthoDB" id="371959at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003217">
        <property type="entry name" value="PRK04183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="GatD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GatD N-terminal" evidence="10">
        <location>
            <begin position="19"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="8">
        <location>
            <begin position="91"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="9">
        <location>
            <begin position="303"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="439" mass="48980" checksum="4D9A7471F0DF1290" modified="2011-06-28" version="1">MLEGYRGSALQSLLQINADIGDLIEIEKNGNIYKGILMPSYSPYDDIIIIKLDNGYNIGVSIISAKIKLIKKKSESQKETQEVSKSTKSEIKIISTGGTIVSKIEYETGAVRPALSTEDIIKFMPEINNIAKISAEILFAILSENMTPEHWVKIAEAVKRAFKEGNEGVVIAHGTDTMAYTASALAFSLKLPGPVVLVGSQRSSDRPSSDAAINLYSSVLLAKKAPFGEVVINMHGESSDTYTLAHRGVKVRKMHTSRRDAFQSINDKPLAKVEWKENKVEILRSDYIPKRDEVEEDAKFDTHVFLLKYYPGLTPDILEYLMEKKVRGVILEGTGLGHVATSFVDYIKKMTKDGVFVGMTSQCLFGRVNMNVYTTGRLLQEAGVVPLEDMLPEVAIVKLMWVLAHESDQEKVKKLMLTNLVGEINPRHSLDFFPRWNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q6</accession>
    <name>F4B4Q6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ordered locus">Ahos_1403</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q6"/>
    <dbReference type="STRING" id="933801.Ahos_1403"/>
    <dbReference type="KEGG" id="aho:Ahos_1403"/>
    <dbReference type="eggNOG" id="arCOG04174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="62"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="367" mass="42428" checksum="BDA3C8830F55EEA0" modified="2011-06-28" version="1">MHYFKLTIMVVSTLRIDTLSRIMEEMEKEKYHIIGNHSAYKKCHWTHEALTEGRYCYKGKFYGIESHRCVQMTPTAAWCWFRCIHCWRLEPEDIGLEWDETKLPITDDPEYIAEKSIEEHKRAVSGYLGRKGVDPNMAKEAMKPAHVAISLTGEPTLYDRLGELIHEYHKRGLTTFLVTSGVRPDVLASLEEEPTQLFVSIQAPNERKHKLINRPIVANSWNLFLKTLEILPSFSSPTVIRMTMIKGFNMSEEDAKDFAKLIEMAMPTYIEVKAYMHVGPSTLRLSRDAMPRHSEVREFAKLMEKYTGYKIISEHVPSRIVLLSKLDKPIQIGNAWSDKWNWATKDTEDDIHGEYAEAELDSGNNQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S8</accession>
    <name>F4B4S8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775010.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4S8"/>
    <dbReference type="GeneID" id="10599641"/>
    <dbReference type="KEGG" id="aho:Ahos_0201"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_1_1_2"/>
    <dbReference type="OrthoDB" id="101044at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="10"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="141" mass="16676" checksum="E5C734D760669073" modified="2011-06-28" version="1">MSGNRVQRLKRRALRFLTDAKNDYNEGYYDTSTFHVEQALQLYIKALIFELFGKDYEGHGIRELLGYLSKLLKENGYEDLSKKVNELVREYREQLISIEDAYIDSRYEDIDYYEEESKELIGVAERIINFLEEVAKNVKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Y1</accession>
    <name>F4B4Y1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Heavy metal translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Ahos_0255</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775063.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Y1"/>
    <dbReference type="STRING" id="933801.Ahos_0255"/>
    <dbReference type="GeneID" id="10599695"/>
    <dbReference type="KEGG" id="aho:Ahos_0255"/>
    <dbReference type="eggNOG" id="arCOG01576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02763">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_2"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01511">
        <property type="entry name" value="ATPase-IB1_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="703"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="11">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="751" mass="81525" checksum="68AA309CBF02DD93" modified="2011-06-28" version="1">MSKEEGEKLRIKKEEELKVVGMHCATCVSTVSKSIKSVKGVKDANVNLASGIARVEIENARLKDIVEAIKKAGYDVVTQKISFKIDINPEDARKIEEKIEEIKGVIKTSVNPTNGYVIVEFNPYSITSDELAEEIYKKTGHKVTKVKSEVTNKSEVKEMLVRLIIAWIFTIPTLYFQYSGLLFIAFLTSIPVQFYAGLRYHLGAYRALKNKTANMDTLVSLSTNIAWFYSVYALIAHQPVFFDVATLLISFILIGKTLEAYLKERISVQITSLKEVKATLADGRVVKAGELKVGDETIVKSGEVIPADGIVDEGSGEVNESIVNGESLPVKKEKGDAVIGGSTLISGYLKVYVTRAGERTYISQVVEAVNQAQTARLPIQNLVDKVASIFTPLIIGISAIVFAVWKFILGFSTEESLLFSVATLASACPCPFGLATPLAVLTSVNRLAKKGIIVRNGESLETLKKVDTFVFDKTGTITEGKISVRAEGDKGSISYASALEKMSNHPVAKAISSISNGAFEVKDFTEFPGSGVYGKVNGHDVIVGKREFVLQNCEGEGKGDVLVCVDGKVSASFYLEDKIREDAVKVIDYLKRMGKRIIVATGDSSDNAEKVGKELGVEVIKGLSADEKAEFIRGEVNKKRIVAFIGDGVNDAIALKEANVGIAISSGTDIAKYAGDIIIPKVGDLLTLLEYSKLTVRKIKENLAWAFGYNSVLVPIAAGILYPTLYLPPEYAALAMSMDSVAVSLWSLVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Y2</accession>
    <name>F4B4Y2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YHS domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Y2"/>
    <dbReference type="STRING" id="933801.Ahos_0256"/>
    <dbReference type="KEGG" id="aho:Ahos_0256"/>
    <dbReference type="eggNOG" id="arCOG04507">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185152_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Ribonucleotide Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
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        <property type="entry name" value="RNR-like"/>
    </dbReference>
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        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007029">
        <property type="entry name" value="YHS_dom"/>
    </dbReference>
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        <property type="entry name" value="YHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRASH" evidence="1">
        <location>
            <begin position="3"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="54" mass="6315" checksum="8701F5C8905D81D7" modified="2011-06-28" version="1">MIDPVCGMEVDENSQYKTMYKGKVYYFCSPHCMKAFQKDPEKYLKEGPKGMPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B519</accession>
    <name>F4B519_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776236.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B519"/>
    <dbReference type="GeneID" id="10600934"/>
    <dbReference type="KEGG" id="aho:Ahos_1438"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118419_0_0_2"/>
    <dbReference type="OrthoDB" id="41609at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="176" mass="20470" checksum="C8749A7F45BF909C" modified="2011-06-28" version="1">MEEKLISPKIYRKAYIKARIIETLDDIDVAIHMWIAGRSRNSAGKVFSAVKALLSALVTKNLDRLPNSDWYAKRGYNALTHSLKGISMDLAKLGFTQVENITDKAFLLHDYQYNGFDLDFSKYKKKEEVLHDILIVSNFILDHIKEWFKEEWDNDLDNVYNIVLYDMKKLSCRGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B563</accession>
    <name>F4B563_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylglucosamine, MurG, LPS N-acetylglucosamine transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776280.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B563"/>
    <dbReference type="STRING" id="933801.Ahos_1482"/>
    <dbReference type="GeneID" id="10600982"/>
    <dbReference type="KEGG" id="aho:Ahos_1482"/>
    <dbReference type="eggNOG" id="arCOG01394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_873241_0_0_2"/>
    <dbReference type="OrthoDB" id="17804at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015:SF22">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--N-ACETYLMURAMYL-(PENTAPEPTIDE) PYROPHOSPHORYL-UNDECAPRENOL N-ACETYLGLUCOSAMINE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="2">
        <location>
            <begin position="166"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="313" mass="35662" checksum="908D77D98FA5DF00" modified="2011-06-28" version="1">MSDILIIASGGGHTGFARAIAEYLPYKADFIIPKGDEMSKKMISQFADKIYEVEKFRSPSGNLSLSSFLLSMIHSAKIRKYKKVIATGSNHSIFPSFFQFVKSSNIYVIESQDRIVTKGKAVNIISKYSKHVFLHWKEQEKLYKNGIVVGPIVERPKYTPSDEGYILVTTGSEGFERLFDILYSLDIDNVVLQTGKVNKKYEKKGWKVFSFDPDIERYIAKAKLVITHQGKTAMEAVVMYKKPTIIVYNKDWKYAATFEDSKLYAEILGTVFLNDPSNWSTKDEILKYIENPKQPKVYTPGTERLVSVVLNDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B565</accession>
    <name>F4B565_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Succinyl-CoA synthetase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776282.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B565"/>
    <dbReference type="STRING" id="933801.Ahos_1484"/>
    <dbReference type="GeneID" id="10600984"/>
    <dbReference type="KEGG" id="aho:Ahos_1484"/>
    <dbReference type="eggNOG" id="arCOG01337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_2"/>
    <dbReference type="OrthoDB" id="146449at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp fold succinyl-CoA synthetase-type" evidence="6">
        <location>
            <begin position="2"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-citrate synthase/succinyl-CoA ligase C-terminal" evidence="5">
        <location>
            <begin position="220"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="335" mass="37351" checksum="FDB7E2B3482AAFC7" modified="2011-06-28" version="1">MKLYEYEGKLLFSKVGIKIPHGIVTEGNIDWKGKAVVKAQILEGGRGKRGLVKVTDDVNSTIQEMRKLGINKFLVEEYIPHDREVYLSMILDRDTAEPMLIASPHGGIDIESSGEVKKFIIPIERGPRGYDIIEIEKYLGVKGLFQVIQGLYKIFTEYDAELVEINPLAVTDNGVIALDSKVILEDNALYKHQDFLKEINREYSPDSYVELDGDIGIIGNGAGLTMATMDMVKLMGGNPADFMDVGGGADRERVRYCVIKVGSNPKVKKILVNIYGGITRCDEVALGIVDAYNVIKKPIFVRLVGTNEEEGWKILKEHGINFYENPIDAVRDALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B567</accession>
    <name>F4B567_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B567"/>
    <dbReference type="STRING" id="933801.Ahos_1486"/>
    <dbReference type="KEGG" id="aho:Ahos_1486"/>
    <dbReference type="eggNOG" id="arCOG08212">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187342_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="80" mass="9577" checksum="E99B39CB285B4907" modified="2011-06-28" version="1">MICKSFKKSKKRFYLMKEREVEAKRLVGKKLVRGKVYEYEYYTLPLNLYIPKSMVEKFGTKYMLEVDEDNGTITIKPRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B580</accession>
    <name>F4B580_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775090.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B580"/>
    <dbReference type="GeneID" id="10599723"/>
    <dbReference type="KEGG" id="aho:Ahos_0283"/>
    <dbReference type="eggNOG" id="arCOG07721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870600_0_0_2"/>
    <dbReference type="OrthoDB" id="38668at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="136" mass="15866" checksum="FD35D2C360E21F7D" modified="2011-06-28" version="1">MAGKFLFITKDKKFLFDGKVREVKKELQDLDGMEIRFARPMIVYELDGVNLNYFVKNYGHLAVGDYTVLDLVDLLEENNFILYVDHEKRKVEVFVQGKDEVITLPYSTLDFLRYLLAKTSRGVLLESTTFDLIDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5F5</accession>
    <name>F4B5F5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CoA-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776294.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5F5"/>
    <dbReference type="STRING" id="933801.Ahos_1496"/>
    <dbReference type="GeneID" id="10600996"/>
    <dbReference type="KEGG" id="aho:Ahos_1496"/>
    <dbReference type="eggNOG" id="arCOG04227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112567_0_1_2"/>
    <dbReference type="OrthoDB" id="42776at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="12"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="142" mass="16522" checksum="B9C670B6DCCB4623" modified="2011-06-28" version="1">MEREENLIKFILENYKNIATIGFSKDPSKPSHYVPKFLMEHGYNVIPVNPTAKEILGKKSYSSILEIPDRIDVVEVFRPSSEVPKIVEEVIKRKREKGDVKVLWLQEGIRNDDAAKLAEKEGIIVIQDRCMYKEYNKLMQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G7</accession>
    <name>F4B5G7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1508</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G7"/>
    <dbReference type="STRING" id="933801.Ahos_1508"/>
    <dbReference type="KEGG" id="aho:Ahos_1508"/>
    <dbReference type="eggNOG" id="arCOG05331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_591375_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="403" mass="44174" checksum="6ABE5802AF73A247" modified="2011-06-28" version="1">MWVGLIIGFIIGAAAEVWGISNPETLIRLAKWEDRLFVTCIALGFAIGTPVLFGLYAAGVGFHWGPKPFYVIGVLIGGLFFGAGLALSGYFPGSIWMALGEGRRDAVYAVFGAILGAATWTALYQTPAGQWLTTVGNFGSLIIGATHPSGKEYLIPWDGLTPLDLFGISIIYAIGMFLLAYYLPRYKGGVRSCLRASIERRLTPQEIQMHQETARFLTEGGLPYSENSLAKKLNEYYAVEDNVTRWFMVSVAGIVALTVVAEMFLHQIFGESTTDSWIAGHLFMPTFKYSQIVFKGIGWEPYSDIGTLMGAFFSAIFLTRRFTAFRDIIPPSWAERFGTNPAKRFLGSFGGAYLMLFGARMADGCASGHILSGDLEMALSGLEFTAAVFASMLITAHYVYKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H7</accession>
    <name>F4B5H7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1518</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H7"/>
    <dbReference type="STRING" id="933801.Ahos_1518"/>
    <dbReference type="KEGG" id="aho:Ahos_1518"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204:SF18">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="4">
        <location>
            <begin position="30"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="124" mass="14156" checksum="D49F5CA43185ED84" modified="2011-06-28" version="1">MVGTMSPFGGNPRPFQGGEEVSLKKLDTICPIVETIRIIGSEPKLLVLRYLLDGGKGFNELQRLTKLSLKTLSSTLKDLENNDLIKRMIISDRPFRVRYELTEKGKELKGVFEEIQKWGNKYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5I5</accession>
    <name>F4B5I5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1526</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5I5"/>
    <dbReference type="STRING" id="933801.Ahos_1526"/>
    <dbReference type="KEGG" id="aho:Ahos_1526"/>
    <dbReference type="HOGENOM" id="CLU_3302529_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="39" mass="4458" checksum="9BF1C0A4DD396AAE" modified="2011-06-28" version="1">MFCVISNKIVDFTLPTVYIPSKLYSSEAIFKNNYFPGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5L2</accession>
    <name>F4B5L2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved Fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776351.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L2"/>
    <dbReference type="GeneID" id="10601053"/>
    <dbReference type="KEGG" id="aho:Ahos_1553"/>
    <dbReference type="eggNOG" id="arCOG12065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893115_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="311" mass="36391" checksum="1DBC71CBB1C09DAB" modified="2011-06-28" version="1">MSQNNAKTNANSDSETNNKKISNNDSVIQLAVIQPRYNTNSNATTLSQEEEKNSLENSHSNNLLDRDKNGNIENLLQDKEKFEIIEILHILDRLKGKLSGDSYLVIRVSNAFKELYEKYYAPKKPTLSPQTENIRRNILYSILDFLYETKFSKQDIKIQELFFKRCEDLGYPIGKVKKIKEEKPPIIVVQHIEENSEKEPKEKELKDEFKQKFKEFEKFLNELIFTPDNLINNAYLITKPLVKKLFQYIDELVNMDSTPREVKQLLIEMKDYLEKYPLNEKRFINGIASIPTGVHKQEITPKLTKIEKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q8</accession>
    <name>F4B5Q8_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AsnC family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775189.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Q8"/>
    <dbReference type="GeneID" id="10599824"/>
    <dbReference type="KEGG" id="aho:Ahos_0384"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066382_0_0_2"/>
    <dbReference type="OrthoDB" id="42020at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="41893" checksum="320BCC7932E127DC" modified="2011-06-28" version="1">MLIEKEYLKLSEILYYLQRFGDLNPKIISAISKIRDAEKLIQWLRENYHYRLYPFPRYEDLGLKKYYVTIYSNYYRISLLDLYDTFDGITTFIFRDVNNPLVINAAIYFTSTSFDKVLDYLQDEGIIFHYDIHKVDEEKFYPIDYSIFNFNKGVFEGLLSSPRKPFELPDLTEGFSPDDLDVKILGKKQERADISLKDLSSLLGVSFKDVLYHTQAHVIGKGLIKFYSVYLYKPDFRLEVSFKEEEVLEELSKIPSMYYVYKLDDKSYVANIRDSSSTLLSYLKFISSLKEKDTIETYLHPYDQQYMITASIPYEHFEKGRWNFNTEVMMLRAEKLVKKIEKEEENKKEEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5S5</accession>
    <name>F4B5S5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0402</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 11">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775206.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5S5"/>
    <dbReference type="STRING" id="933801.Ahos_0402"/>
    <dbReference type="GeneID" id="10599842"/>
    <dbReference type="KEGG" id="aho:Ahos_0402"/>
    <dbReference type="eggNOG" id="arCOG00329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491446_0_0_2"/>
    <dbReference type="OrthoDB" id="8639at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="8">
        <location>
            <begin position="229"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="554" mass="64203" checksum="9BA26C3FD43B474C" modified="2011-06-28" version="1">MVEGYLIDAKPIRGGLILYLNNFKKAFVKTTFPVYVITENPEMVMQHPAVVSYEEEEWNYQGKKRKVYRFEVDDISAYYYIRNKLNVVNETPSVLSQTLYRLGILPFRWVSIEGNEIKTRDDDFPNISYATLIPLDWYGEGETGSKIKVIENGEERVEQNPKVKVDVAECLGIACNNVDAVVKIDIKRKRSPVSIKGLIEWSYDSKVLLREIAYATIGKALTTNEAWVALKRKYIIPKVVPRVEKMRTIEELEKADRGGLVIFPKVGCFNNVYQIDFSSMYPSLIVKYNISAETVDACEDLKTEIGHSICLKEKGIVPEALEWLIKRKEELKKIDEERAEAVKWMLVASFGYLGFRNSKFGKIEAYELVTYFARKTLRQAIELAEEMGLEVLHGVIDSLSVRGNVKDYVKKLEEITGLKVKVEEFKWMIFTKNKEGFPYPTRYFGKGEEMKVRGVIRSNMPNVVKDFLEEVLSSLSKANSCEEINVKEIDEIYKKYRERIVRGEPKDYVIWIDGIPYVRGIKGFYEAYNGFRGVDVFYYLNYLKRTYEEFKGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5W7</accession>
    <name>F4B5W7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776380.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5W7"/>
    <dbReference type="GeneID" id="10601083"/>
    <dbReference type="KEGG" id="aho:Ahos_1582"/>
    <dbReference type="eggNOG" id="arCOG07723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2629888_0_0_2"/>
    <dbReference type="OrthoDB" id="43107at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="81" mass="9359" checksum="6E44A0DD9B126047" modified="2011-06-28" version="1">MSEIIRVSKDVKEKLVKIAAELQLNKGKRVSLNEAVEYLITFYEENKKFQKNVQLLFSLLGSAKGIREELERSRREDEGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B603</accession>
    <name>F4B603_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universally conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B603"/>
    <dbReference type="KEGG" id="aho:Ahos_1623"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295:SF18">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33295">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4143" evidence="2">
        <location>
            <begin position="138"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="343" mass="40146" checksum="06558BE13F8B5676" modified="2011-06-28" version="1">MYELYLTHVKGNADKITYIAIDEAQEIEGWERFVRGLEEKCKAKIIVTGSSAKLLSSEYTTLLSGRHVEVRVFPLSFDEALKFKGIEFDSELQLAKNLDRIKKELVEMLNYGGFPDVVLHPEVRSELIHSYFDTVIVKDVVNRYNLRREEKIRNLAKFYISSSASRITYNSVSKFLKIPVKTVERYSEYLEKAFLIFFTRNYSQSPKAVENSPRKVYVIDNGFLKFFSTWSLGRAFETLIAQHVMRYANRRLFEVFYWYSQNAEVDIIIKNDKGSLLPIQVTYELNEENRKREIDSIKKFSKYGKTRPLIITFDQEGEVEGIKIIPAYKFLLKTFEILDSMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B611</accession>
    <name>F4B611_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776424.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B611"/>
    <dbReference type="STRING" id="933801.Ahos_1631"/>
    <dbReference type="GeneID" id="10601131"/>
    <dbReference type="KEGG" id="aho:Ahos_1631"/>
    <dbReference type="HOGENOM" id="CLU_2911713_0_0_2"/>
    <dbReference type="OrthoDB" id="37890at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="62" mass="7392" checksum="41925EFF0F0C7341" modified="2011-06-28" version="1">MDDLRAKNVITFTDVGECTNCGVYGPYEFVFWSSHTRRVYGFCSLKCFREWLRTTRAIYPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B662</accession>
    <name>F4B662_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD-superfamily hydrolase, subfamily IA, variant 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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    </evidence>
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    </evidence>
    <sequence length="217" mass="25405" checksum="E8038F366AB82B3A" modified="2011-06-28" version="1">MPYAISVDLGETLVGFRPRSYEYMLGILKDYGYKIDERSFFRALVKVLGKSNFANEEGLNPLNLHELFYEIGIHPCRKIMEEIANRDKYQDYFLYEDAIDFLKEVKKKYKVVIISNSTKRIHKIVKEFELDKFVDKVVASCDIGIVKPNPRIFAYAKRYVGDILLHVGDIYELDVVGAKRAFIDAILLDRFNFYPEIKIKAKTLYDVLRYMEIKGML</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F4B670"/>
    <dbReference type="KEGG" id="aho:Ahos_0470"/>
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        <location>
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            <end position="47"/>
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        <location>
            <begin position="59"/>
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            <dbReference type="EMBL" id="AEE93358.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="93" mass="10854" checksum="6671F3522D61F3E4" modified="2011-06-28" version="1">MIIDFNFYYLRLGLYKVIPKEKFATTLSMTNTIVALPIIMFTVLSSFPIQILKYYNLELIILFIEITISIYSVPTLIKLIEIKENSNKDSRNV</sequence>
</entry>
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    <accession>F4B6B5</accession>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="KEGG" id="aho:Ahos_1657"/>
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    </dbReference>
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            <dbReference type="EMBL" id="AEE94535.1"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <sequence length="167" mass="19330" checksum="C2FF37FEC72AF5BA" modified="2011-06-28" version="1">MCDLKFGKVERPILDNKVVLLRKELFEKLADEKFMYVLSTHDKGNLSKSYFYLIVDKLREAGLLMENAISFSAVLPFKASNEGIWFTDGLLYRKDKELFYINMESDKLTCSSCPIFSQCIYAIKTIAKGHDIPLIKENPREEWKLIIDSLKKEFGEKVASLKILSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B7</accession>
    <name>F4B6B7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sec-independent translocation protein mttA/Hcf106</fullName>
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    <gene>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
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    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B7"/>
    <dbReference type="STRING" id="933801.Ahos_1659"/>
    <dbReference type="GeneID" id="10601159"/>
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    <dbReference type="eggNOG" id="arCOG05911">
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TatA/B/E"/>
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    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022927"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="110" mass="12798" checksum="39F899EE0FEC7B35" modified="2011-06-28" version="1">MLGNISDTAIIILVAILLLAGQKDVAGTVRNLGKTLEEMRRRQNEFKEELMRELSETGEVTNSIKKDFSDEPIIRPYHQPTQDYKIKQLEEEIKRLQSELERLKNSDRKN</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <begin position="57"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        </citation>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11870" checksum="72277123131623BA" modified="2011-06-28" version="1">MEYAVISPNLFKRLDEVVEKFKGKYSLIITTSGLSFALKKGIDVDKALDEGVKVLAYSHKFQPLEGLSVEETEAVLLAKDLNYYLITADDKVKEFAEKEGVKVIII</sequence>
</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
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        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="157" mass="18192" checksum="10A1778FE59A5331" modified="2011-06-28" version="1">MEIPEEVIKKAEEKGINVTDLILMELSKKDPSEAIKIRLDLAKKYMAEAEEYLKKGDAVQASEKAYKVAEEVVKALSEKFNLQEYQQAVKEGRWYTYTLGESASILSEKLGDWVLEGWSNAYFLHVWGFYEGKLKVEYVSRYINPIKRMLEEAEKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="139" mass="15282" checksum="FCB8DB8118241D1C" modified="2011-06-28" version="1">MYKVIFKVTKGFANNSSQSLRKIGFIPIYRKIQGDYDMYMTLFKGNNLAEVKEAISEAAFYFAKEGKFGRQDFATIYEVDDKYLGKGIGSLLGAGLGYKIAGLPGLILGLLGGVIIGELADITLGEKFVGVMEWPKLSY</sequence>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    <dbReference type="KEGG" id="aho:Ahos_0578"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093718_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="InterPro" id="IPR018693">
        <property type="entry name" value="DUF2192"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="28936" checksum="17FD96DE43DDE87B" modified="2011-06-28" version="1">MVCSLNAILFKQNYTIFNGLIMVKEIYKGRIKVITDLWGKILDEWESLNRDSLISLVQKIYEDNGIKPFRGFKSTNLYEKELISVYVVGKEGLGLFDDYKQVFDKLFSTEENYENIANLILNSPDEAFKKANEDKDLLARALRLIFVEVVFSFADESKLIDALRRLDASTNDAIKHTAKSFSRFYTAFKLAEGIAEGSIRDKMNYIAMKKAISINIGIEYPLPKANYVALISQEVFNVKPRLIKKILEVSAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S6</accession>
    <name>F4B6S6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CRISPR-associated HD domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1742</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776534.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S6"/>
    <dbReference type="STRING" id="933801.Ahos_1742"/>
    <dbReference type="GeneID" id="10601242"/>
    <dbReference type="KEGG" id="aho:Ahos_1742"/>
    <dbReference type="eggNOG" id="arCOG01442">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1163752_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10013">
        <property type="entry name" value="Cas3''_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18019">
        <property type="entry name" value="Cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="238" mass="27788" checksum="70B034914B88FB8E" modified="2011-06-28" version="1">MVYLSKPCAFIKQTLFDHSMGSYAVLSKFINYTYYAVISRRLKSVGIQLKPEEVKRLIEISVKLHDIGKAAEYYQNQFDENCLSNKKEPSFIFHEIGSSLFFYYSNYTNDNKVKRLLALAALNHLNAIRGLADFKPNQLPKGAKEEMLKIEKYGKVLLSKLGFNFEVRDYTTDDYIKMMNDFINRPKDEDLKLYKLHNLFLAPIIIGDNLDSHYARSISERRRFIKLLEVELNEDSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S7</accession>
    <name>F4B6S7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CRISPR-associated helicase Cas3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1743</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776535.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S7"/>
    <dbReference type="STRING" id="933801.Ahos_1743"/>
    <dbReference type="GeneID" id="10601243"/>
    <dbReference type="KEGG" id="aho:Ahos_1743"/>
    <dbReference type="eggNOG" id="arCOG01444">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031100_1_0_2"/>
    <dbReference type="OrthoDB" id="43851at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052511">
        <property type="entry name" value="ATP-dep_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054712">
        <property type="entry name" value="Cas3-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962:SF5">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47962">
        <property type="entry name" value="ATP-DEPENDENT HELICASE LHR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22590">
        <property type="entry name" value="Cas3-like_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="27"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="233"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="485" mass="55061" checksum="9CE81566E9428DCB" modified="2011-06-28" version="1">MSSLVDYYNDFIYKNKLEERKGIEQTLRKLENGENVILKAPTGYGKTTLTKILANAVERGNEVGSRVIHVLPLRAIVQDLYIKLMKDSKDGKIYTRSIGAQDMDYFSSPFFMKKVTVTTLDTFVMNLFKLPPVESRNIFNNYGSHYELPRAMIYSSIVIFDEAHLIGEEGRPLTAFLASLRVLSEVGVPIVIMSATIDEGLEKTIKNYINVETIEANDFSIKRNITVSKIDDPIKDARNQVKEGKRVLLTFNTRRDAIDSYMRLRDLNPVLIHSKFNRFDRIELMWEVLDRNTKLVISTQVIEAGIDTTFDVLITEAAPAHNLIQRAGRVARYGGNGEVHVFPFRGKVYDEKEVSDTYNRINNGVDDSLLVRREYKVDNTLLHDLTVIDSIYADAKRAKSLYEQVCNIVRDTSIILGFPPHSDSTMFAIPLSKDEAKKLIEKGYSLVPEGRSLNTLNCLQLQMIKNSILGVRIKGYSREIGGILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T8</accession>
    <name>F4B6T8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6T8"/>
    <dbReference type="STRING" id="933801.Ahos_1754"/>
    <dbReference type="KEGG" id="aho:Ahos_1754"/>
    <dbReference type="HOGENOM" id="CLU_2366037_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="95" mass="10963" checksum="62B4655889E2BD12" modified="2011-06-28" version="1">MIFSLILLGFLFSCLIWLMVLGQIGMMYLFWVRGRLILCVLGMILVKLLVCLVVMLFFIILIMSLVGIWLMLMSVGLILGFPYWFTMLMMMLLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6U8</accession>
    <name>F4B6U8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94641.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6U8"/>
    <dbReference type="STRING" id="933801.Ahos_1764"/>
    <dbReference type="GeneID" id="10601264"/>
    <dbReference type="KEGG" id="aho:Ahos_1764"/>
    <dbReference type="HOGENOM" id="CLU_1507298_0_0_2"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="178" mass="20913" checksum="1F21ACC0CB2F3DB3" modified="2011-06-28" version="1">MYDKLSTSEIILLTLREKGGKVDIDKLQEIIKEFYNSKCTSEICEKIDPNDKKLIANSLYYLTIVGLVTLEKEKIDSKEVYEIIKKYKDYLFSPVVLYYPYLGRRFVLPPVLPEKFTRRDIKNLILFLEGLKENEPSLKIENDIEKLKMLMKTRTFVKLTKDGEEIASKLLTEYKVKV</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
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        <property type="entry name" value="PaREP1"/>
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    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
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    </dbReference>
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    </dbReference>
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            <dbReference type="EMBL" id="AEE94645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="156" mass="17898" checksum="ACECE41FA4E350D9" modified="2011-06-28" version="1">MEELIKKAEEKGIDVEDLIIFTLSKEDPSEGIKMRIELAKKYMAEAEEYLKKGDAVQASEKAYKVAEEVLKALAEKFNTPEYQQAVKEGRWYTYLLSKATNTLSSTLGDKIIKGWSSAYLLHVWGFHEAKLETKDIMSYINVVKEMLEEANKYLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6V5</accession>
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        <submittedName>
            <fullName evidence="2">Conserved pro-fuselloviral protein</fullName>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="GeneID" id="10601272"/>
    <dbReference type="KEGG" id="aho:Ahos_1772"/>
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            <begin position="91"/>
            <end position="110"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="236" mass="26500" checksum="ABA56600BEF52E9B" modified="2011-06-28" version="1">MRKIVVSILLLSLSVLVLYSAIYLHSLDKTTVRTSVYNITITTTTTYNGPIYGTPNINYGCPIQWPPPLTGTTVTHVTETTIVQPPSYASLWPYILALSIIILGLAVLIFRRNYCMSKVMILLISLILISLAVFLYSTDKPIVHILVLSKVTCNKNNYIQLAVVHTYNSADCPLCEPKPPVIYQTVIILPPPWYAQLWFLPLTVGILLIFMLAYMCKAVTLKVLKVIIKYLEPILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Y0</accession>
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1797"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_465116_0_0_2"/>
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            <begin position="12"/>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94673.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="586" mass="65859" checksum="3013BF62D56B94CD" modified="2011-06-28" version="1">MVNSEMLKRIASNLLLSIIFVSMVAFMSIHSYATNEGRSIIIHVIDGEKPIYNARLQIYVFTPLGLVYLTTLFTNRCGNAILQYSSLLSYVGTAINFKSIGIQIMGTYFNSTTKVLHYNVKSCIIPIAEIVHPNATKIITLNLHYKKVIKKAHDNNQNIHKIIKPMSNCPPIPPSPGPYYEWVLVKQQSINNVPIPFAWGKQCTPGQSIIYFLCESSSSATVCTWAAGSISICGAPISFKIVGKSAQVCSTLLHMPLYLVRYCNGTGYPCGMLYANATIGLAEFQLYYVYPCSGYAYPCNGYFNETYLEHFDTNLLYSLPSTARYPNYPWIYQELEPILSTYVKSVPYIQSAKIYYMQRIFFFSFQNYSRVCNVYLPLGLAIPVGDIIVSLFVPEIIPIISPDILATLIIGITATQNNQYIRLVCLDLLSLKCKSYYCVDIEVGYIPQEYYYNGFNTVFPFYYFKIISPESMCVSTNILTPTKPCTPPGIYVPPGGNLRFTLNITLGGTDILAPHGTICVMVIPVGQNPTWTHRYSVDGNTLTICVPSGVLFPYGTNWTVTIIYYRTSNGTIIYKCSCTWFYAYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776590.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Y2"/>
    <dbReference type="GeneID" id="80457674"/>
    <dbReference type="KEGG" id="aho:Ahos_1800"/>
    <dbReference type="eggNOG" id="arCOG03712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2874870_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="63" mass="7354" checksum="BF3C385EBE4E8DFF" modified="2011-06-28" version="1">MEITKLLDIKDRRTYASLRLLECLTEIRISLEMLKEGYSRNSAQKCFIAWKAFLSELASLNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z0</accession>
    <name>F4B6Z0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1808</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776598.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z0"/>
    <dbReference type="STRING" id="933801.Ahos_1808"/>
    <dbReference type="GeneID" id="10601308"/>
    <dbReference type="KEGG" id="aho:Ahos_1808"/>
    <dbReference type="HOGENOM" id="CLU_1222466_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="226" mass="26428" checksum="E729B5003D2FCD43" modified="2011-06-28" version="1">MDASLIKLLSKESKMKQEEIEERLSSASKAIQYVISFNYEEKYTESVTLSGNYKFIRLKPFNRLSFKKVFLSTSSKILEGQVKMNAEGKGEVKIKDLGNFQNEIEITFNGKGKEMEVYELEFKVIFTNELLKDIKKYLEIQEKVYGTRRLFSINFNVMLSPILRYRQSLSFPRLKEAMAIEAKYLSKKGFPEKFKVLDFLKVNKGLTEFLIDFPDFGYIGIIYEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6Z9</accession>
    <name>F4B6Z9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Large ribosomal subunit protein eL8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rpl7ae</name>
        <name evidence="11" type="ordered locus">Ahos_0594</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775398.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6Z9"/>
    <dbReference type="STRING" id="933801.Ahos_0594"/>
    <dbReference type="GeneID" id="10600040"/>
    <dbReference type="KEGG" id="aho:Ahos_0594"/>
    <dbReference type="eggNOG" id="arCOG01751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="OrthoDB" id="25810at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000020">
        <property type="entry name" value="50S ribosomal protein L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_eL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_eL8_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="18"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="125" mass="13479" checksum="89F46A8E0CA02D40" modified="2011-06-28" version="1">MSKPSYVKFEVPQDLVDKALEVLKKAKESGKIKKGTNETTKAVERGQAKLVYIAEDVQPEEIVAHLPGLCEEKKVPYIYVPSKKALGEACGLQVAAASAAIIDPGEAKDALDEVLKKLQEISGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B739</accession>
    <name>F4B739_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleotidyl transferase (DUF2204)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775438.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B739"/>
    <dbReference type="GeneID" id="10600082"/>
    <dbReference type="KEGG" id="aho:Ahos_0635"/>
    <dbReference type="eggNOG" id="arCOG04119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120784_0_0_2"/>
    <dbReference type="OrthoDB" id="26089at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018700">
        <property type="entry name" value="DUF2204"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09970">
        <property type="entry name" value="DUF2204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="187" mass="21600" checksum="D592392E48ED12F3" modified="2011-06-28" version="1">MISFNSVGEVLEELKKISDFVIIGDTIVDLLLKRKGTESDVDIFPTEISAIAEGEKYRELAEEKNWDFGSTPIDTPRIIVPVEEGQLQIDIYDNIQDFFVPQEILNSAEEMKIGKSTFKVIRLEDYILLKVNAYREEDEDELKGILYYIAEGKLKINKDYLEKHIELFEENADSIKERLKDIGFKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B746</accession>
    <name>F4B746_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </evidence>
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    </evidence>
    <sequence length="187" mass="21569" checksum="F1A91CB9DDAEECFB" modified="2011-06-28" version="1">MENENNGKRQPCFMRKDIYDEIRRLTELNGSSIYNYTNDLLAASIQMEKNGVDIKNEISTFILLSKLMNNYKGFMILVPFDSLRDSQMSLEKWKELGKSLMSYVSQLMNNVEVSFLSILRFFLSFIGDVNVDYNGKYIESFSPYFQGRIIDFIKSLIDGMLEVSNLKADVSATTGMIRVKLISKNEK</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        </citation>
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    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="aho:Ahos_1832"/>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="AEE94707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="41" mass="4359" checksum="D78EEBEBF0AB8519" modified="2011-06-28" version="1">MEMYKLNLGIRLAIALLGLALATLGIAHVFFAEPSDSGLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D7</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1884</name>
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    <organism evidence="2 3">
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        <lineage>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="933801.Ahos_1884"/>
    <dbReference type="KEGG" id="aho:Ahos_1884"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
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        <location>
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        <location>
            <begin position="146"/>
            <end position="169"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="265" mass="29653" checksum="08CAFF795F60CC42" modified="2011-06-28" version="1">MKGTSFFSSSLLRYFFLMLAGFFIIYISPSSPDYLLYSTVGNLIFWSSLLILLIKTVLRKPLHYLKKGRTALIIFFSYLSIHYFVYSIALERLLTGFYGILFTVQNPFITFSFTPLYPAGLYTAIMNLVFNPSIAGGFPPNYYFELSLYAMVMGLIIATLVTANIMRVLEISSRLTRAKVILLAPLLGVIGGGSCCISIPIILATVIPTANSLLFLPIGDTALFLAYILLPPITAIGLKLNYDALFPKVPKEFRIKDEMITKRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7K1</accession>
    <name>F4B7K1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalamin-independent synthase MetE domain protein</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">Ahos_0719</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0719"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:amino acid biosynthetic process"/>
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        <location>
            <begin position="82"/>
            <end position="285"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93606.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
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    </evidence>
    <sequence length="319" mass="36994" checksum="05D9D446B1621880" modified="2011-06-28" version="1">MIKFKACIIGSYPKSLALGKTISRYRSGKISQDKLENAILKEEEKFFNIVRDVNPECTTDGLFRWDDIVDLTFSYINGQEKGELMRFFDNNFYYRKAVIKSKLTPKDEYKNILEVDREMMKKLGLKSGLSAVILGPLTYVELSQDEYYHNVEELLFTYSSVVNEVIKGIESKVDMIEIHEPSLFSKGIKKEILDKLSSAYESMLKGIRKTTLVISYFEVLNKRLPYLFSLPVENYGVDVIENKKKLANIYKYFKDKNVFLGVLNTRNTKMERISTIRRMIEKAKEKGAKEIMIGNASLMDFIPEKIAKKKLMLLKKVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7K2</accession>
    <name>F4B7K2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase sliding clamp</fullName>
        </recommendedName>
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            <fullName evidence="4">Proliferating cell nuclear antigen homolog</fullName>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pcn</name>
        <name evidence="9" type="ordered locus">Ahos_0720</name>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7K2"/>
    <dbReference type="STRING" id="933801.Ahos_0720"/>
    <dbReference type="KEGG" id="aho:Ahos_0720"/>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002220">
        <property type="entry name" value="PRK01115.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="8">
        <location>
            <begin position="131"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="250" mass="27850" checksum="1C42925EC847A527" modified="2011-06-28" version="1">MLNSIMKFSYDDVRDFKNILTALAKLVDEASLKIKPDGVELVAIDRAHISLVKLQIPKEAFKDYDVQEEFNFGFNTQYLLKILSTSKRKESIELEANDPSQVIIRISGGFNRDYVVRNLDVSPPEVPELNLEFDVNASINSSGFKKAVSQISAVSDTILFKADENGITLKSKSETDVEVEFTKESGGLQDIELSKPAESTYSCDYLDDILALTKLSGLMKISFSDQKPLQIQFNMESGGNVIYLLAPQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7K9</accession>
    <name>F4B7K9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">GTP cyclohydrolase III</fullName>
            <ecNumber evidence="3 4">3.5.4.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gch3</name>
        <name evidence="5" type="ordered locus">Ahos_0727</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29" evidence="3 4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775530.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7K9"/>
    <dbReference type="STRING" id="933801.Ahos_0727"/>
    <dbReference type="GeneID" id="10600181"/>
    <dbReference type="KEGG" id="aho:Ahos_0727"/>
    <dbReference type="eggNOG" id="arCOG04202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080076_0_0_2"/>
    <dbReference type="OrthoDB" id="25211at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="235" mass="26708" checksum="F439E94F64A7D245" modified="2011-06-28" version="1">MKILLMEFIGYKEWTESLGYDREWKIQDIQSSIYKVVNDEISRVGGFALPVRYDGLIILADGIPSGSLLEIYTKVKEISPSKVSACIGYGKTPREAEINAHNCILNSLSGEPKFDTFYDDNVVACHFDYNNFTLLTRELSFYNAYMRIMRLYSKLSEIIYDFGGITQYLGGDNMIAFIDEDTLDKIIKIVENMDEIKVGIGIGKNARSAMAGATRALDEIRSKREDKWKIVKLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X3</accession>
    <name>F4B7X3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="10">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">purP</name>
        <name evidence="13" type="ordered locus">Ahos_0764</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + formate + ATP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775566.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X3"/>
    <dbReference type="STRING" id="933801.Ahos_0764"/>
    <dbReference type="GeneID" id="10600223"/>
    <dbReference type="KEGG" id="aho:Ahos_0764"/>
    <dbReference type="eggNOG" id="arCOG04346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065084_0_0_2"/>
    <dbReference type="OrthoDB" id="98133at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009778">
        <property type="entry name" value="PRK13278.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF2">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
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        <location>
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        </location>
        <ligand>
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        <location>
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        <ligand>
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    <sequence length="334" mass="38122" checksum="32145AB83A9BC6B1" modified="2011-06-28" version="1">MIISTLGSHSSLQILYGAKKEGFTTSVITDKNRENFYKRFNFIDEVKSYNSLDEALDLINKNNEESVFIPHGSLIEYLGMDRVKRIKTPIFGNRNLFEWEANQHKKMSLLKMAKIKIPESFESPEDVDRLVIVKLPGAKGGKGYFIAKNKEEVKEGLNKLLEAKLIKSIDEVIIQEYVIGIPMYFHFFYSPIFKRVEITGMDIRYETNVDGLRRLPTNLISVEPTFVVAGNIPVIARESILPRAFEYAENFVNTVKENVPPGMIGPFCLESIVTDNLDIVVFEFSGRIVAGTNLYIEGSPYSWLYWDEPMSVGRRIAREIKISKLENKLGEVTT</sequence>
</entry>
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    <accession>F4B802</accession>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            <dbReference type="PubMed" id="21607549"/>
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="Proteomes" id="UP000008458"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="82" mass="9114" checksum="1C8D2C6ED6DB8A25" modified="2011-06-28" version="1">MSKVIFFGPLVQAFNSKEIEVKGEDVLQVLKNVDKKGIILDLDNNTIRPGYIILINGVDWRIKGGKITDNDIIQIIPINHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B817</accession>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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    <reference key="2">
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
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                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
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            <end position="364"/>
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        <location>
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        <ligand>
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        <ligand>
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        <ligand>
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        <ligand>
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        <location>
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        <ligand>
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    <feature type="binding site" evidence="5">
        <location>
            <begin position="334"/>
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        </location>
        <ligand>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="AEE93694.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="364" mass="41365" checksum="C9FAEC1361DFE008" modified="2011-06-28" version="1">MKAILFSSDEYLDEAKTLSETAGYEIVSIYKLPKKPNPSYYIQEDKLNKLKNENIDAIIVFDLLKPRHFINLNKELGGKMKILDKLLLLLEIFALHAGSKEAQLQIELAKLKYELPIIKDVYKKHKISEQQGLLGAGVYGVESILRLYHRKIAKISKELMHIKELRESQVKQHESNGIPTVAITGYTNAGKTSIFNILTGLHQKVDSSMFTTTAPKRFKISINGQKIMLVDTVGFIRGIPPQIIEAFFVTLSEIKYADIILLVVDISLEDTLILEMLKSSFSTLRELGISGKPILIVANKTDKIPYEEIDRRSDLIYKISNEIYSPIIGIIPFSAKTLYNLSNLKIKIAESLLMYYNQEKREIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>F4B851</accession>
    <name>F4B851_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Precorrin-2 C20-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2003</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776790.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B851"/>
    <dbReference type="STRING" id="933801.Ahos_2003"/>
    <dbReference type="GeneID" id="10601502"/>
    <dbReference type="KEGG" id="aho:Ahos_2003"/>
    <dbReference type="eggNOG" id="arCOG00648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076014_2_0_2"/>
    <dbReference type="OrthoDB" id="23546at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004062">
        <property type="entry name" value="PRK05576.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467:SF2">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="220" mass="24975" checksum="3902703C337E308A" modified="2011-06-28" version="1">MIYVIGLGPGDPELITVKAQRILSEAEVIFVPYSTGTNRSLALEIVEKFAKGEIVTLGFPMKKDVKDEELKEIAKIICEKTNERKVSAFVTLGDPTLYSTFFRVFNFLNCQEIEIIPGVSSITACPSKVKIPLALKNEAIAIIPADRLDLINLVKEKFETILIMKANENLAEIKELLFPYYNIIYARRCYMEGENIHELKGDEDKDYFSTLICKVKENER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B852</accession>
    <name>F4B852_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="5">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiT</name>
        <name evidence="7" type="ordered locus">Ahos_2004</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + S-adenosyl-L-homocysteine + CO2</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776791.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B852"/>
    <dbReference type="STRING" id="933801.Ahos_2004"/>
    <dbReference type="GeneID" id="10601503"/>
    <dbReference type="KEGG" id="aho:Ahos_2004"/>
    <dbReference type="eggNOG" id="arCOG00977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094143_0_0_2"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001556">
        <property type="entry name" value="PRK00377.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="6">
        <location>
            <begin position="35"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="190" mass="20937" checksum="ECC8290DB43ABC01" modified="2011-06-28" version="1">MLGIPDEMFIRDEKIPMTKEEIRVLALSKAKLFHGAKFVDIGSGTGSVTVEAGLYVGSKGKVYAVEKDPNAVELTRKNIEKFDLHNVTIIQGESPDALKEIREEVDAIFVGGSERLKDSLVASNEILKKKGRIVLDAILLETAVKAMEVLNSMNYNPQIIEVIVAKGMKTSKGYAMLSRNPIFIIYGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B5</accession>
    <name>F4B8B5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">psmA</name>
        <name evidence="8" type="ordered locus">Ahos_0828</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 6">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B5"/>
    <dbReference type="STRING" id="933801.Ahos_0828"/>
    <dbReference type="KEGG" id="aho:Ahos_0828"/>
    <dbReference type="eggNOG" id="arCOG00971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="3"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="235" mass="25789" checksum="369F77FEFA07E129" modified="2011-06-28" version="1">MGYDRAITIFSPDGSLYQVDYAFEAVKKGWTTLGIKTKSAVVVLGEKKKASQLLDLDSIEKVFLLDDHVGCSFAGLASDGRVLIDYARNSALQHRLIYDEPITVDYLTKLISDVKQMYTQHGGVRPFGVALIIGGVDNGVTKLFMTEPSGQFMPYQAVAIGQGGYTATEYLEKNYKEDLNVEDTILLALNALKSTLKPGEKLGPSNVEIGYASADTGIFRKLTTEEKIQYLQKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B9</accession>
    <name>F4B8B9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component Rrp42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rrp42</name>
        <name evidence="7" type="ordered locus">Ahos_0832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Contributes to the structuring of the Rrp41 active site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase PH family. Rrp42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775634.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B9"/>
    <dbReference type="STRING" id="933801.Ahos_0832"/>
    <dbReference type="GeneID" id="10600300"/>
    <dbReference type="KEGG" id="aho:Ahos_0832"/>
    <dbReference type="eggNOG" id="arCOG01574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038194_0_0_2"/>
    <dbReference type="OrthoDB" id="30932at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11365">
        <property type="entry name" value="RNase_PH_archRRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000017">
        <property type="entry name" value="Exosome complex component Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00622">
        <property type="entry name" value="Exosome_Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020869">
        <property type="entry name" value="Rrp42_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003282">
        <property type="entry name" value="PRK04282.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="5">
        <location>
            <begin position="36"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="196"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="276" mass="30399" checksum="0128EED9DDD286DF" modified="2011-06-28" version="1">MSITPSNENIVPLIKRESILASLDRGIRADGRKFNEYRPLSITLSYAKKADGSALVKLGDTTVLAGVKVEPEEPFEDTPNQGNLVVNVELLPLAYETFEPGPPDENAIELSRVVDRSLRDSKSIDLSKLVIEPGKKIWTVWVDIYVLDYGGNILDACTLAAVSALYDTRLPKVVKEGDEIKIIKEEKGEKLPMNFPVVTVSVAKIGKHLVVDPDLDEEGIMDAKISLSYTPEGRIVGIQKTGIGTFTLTEIDNVETLARSVSQKLLEELKKQINIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8G1</accession>
    <name>F4B8G1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0874</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775676.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8G1"/>
    <dbReference type="STRING" id="933801.Ahos_0874"/>
    <dbReference type="GeneID" id="10600343"/>
    <dbReference type="KEGG" id="aho:Ahos_0874"/>
    <dbReference type="eggNOG" id="arCOG04055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111362_3_0_2"/>
    <dbReference type="OrthoDB" id="8831at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001617">
        <property type="entry name" value="PRK00407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="7">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="139" mass="16307" checksum="384F35871720A3E7" modified="2011-06-28" version="1">MKKFEFFDNTADVGIKAYGRTLEEAFENSALAVFEVMTDTSKVDPVEERKVTIDGIDLENLLYRWIESLLVYYDSELMLFSKFKVKIDQDNMRLEGEAWGEKFNEEKHERRTLVKAMTYSLMSINKVGDMYEITFVVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J6</accession>
    <name>F4B8J6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="8">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Alpha-IPM isomerase</fullName>
            <shortName evidence="8">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">leuC</name>
        <name evidence="10" type="ordered locus">Ahos_2077</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776863.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J6"/>
    <dbReference type="STRING" id="933801.Ahos_2077"/>
    <dbReference type="GeneID" id="10601577"/>
    <dbReference type="KEGG" id="aho:Ahos_2077"/>
    <dbReference type="eggNOG" id="arCOG01698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="OrthoDB" id="255at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 8" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="9">
        <location>
            <begin position="8"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="415" mass="44902" checksum="50DBE3D098E7F26F" modified="2011-06-28" version="1">MPQTLTEKILSRASGKEVSPGDVIEAKTDIVAFHDLTGYHVIEVMEKAGIEKVFDKSKIVIAFDHLAPPPDIRSAEIQVMIRKFVKKMGLPNFHDINFGIMHEVLLEKYALPGQVIVAADSHTTTSGAVGAFAQGMGASDIAAATITGKTWLVVPTPFKVELKGSPGKWITGKDVALKLLQEFKADYFNGMSIEVYVENPSAFPMDYRATVSNMGIEMNADALMFIPDEETKNYIKTMRGYEPALVTPDPGAKYTDEYTIELGKLEPLVAAPYSVDNVKTVNEVEGKEVDQVFIGSCTNGRLSDIEIAAKILKDKKVKTRCIAIPASYEMFKRAMDLGYIETLVNAGCIVTYGTCGPCLGGHFGVAGPGETIVSTSNRNFRGRMGSNDSKVYLANPAVAAASALEGKITDPRSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8J9</accession>
    <name>F4B8J9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PaREP1 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8J9"/>
    <dbReference type="STRING" id="933801.Ahos_2080"/>
    <dbReference type="KEGG" id="aho:Ahos_2080"/>
    <dbReference type="eggNOG" id="arCOG03721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115256_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010268">
        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="108" mass="13100" checksum="295D73CC08382BFD" modified="2011-06-28" version="1">MYSLQITKELILNYLKEADDLISRGDFVQACEKYYKAAEDFIKYLSKANIRRWDSRALFKESKKFPCVRDLWRSAWKLHVDCFHNYLMDENEIMILISDVRKIRDLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q0</accession>
    <name>F4B8Q0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">[LysW]-aminoadipate/[LysW]-glutamate kinase</fullName>
            <ecNumber evidence="9">2.7.2.17</ecNumber>
            <ecNumber evidence="9">2.7.2.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lysZ</name>
        <name evidence="11" type="ordered locus">Ahos_0884</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in both the arginine and lysine biosynthetic pathways. Phosphorylates the LysW-bound precursors glutamate (for arginine biosynthesis), respectively alpha-aminoadipate (for lysine biosynthesis).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[amino-group carrier protein]-C-terminal-N-(1,4-dicarboxybutan-1-yl)-L-glutamine + ATP = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + ADP</text>
            <dbReference type="Rhea" id="RHEA:41944"/>
            <dbReference type="Rhea" id="RHEA-COMP:9694"/>
            <dbReference type="Rhea" id="RHEA-COMP:9712"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78499"/>
            <dbReference type="ChEBI" id="CHEBI:78503"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-glutamyl)-L-glutamate + ATP = [amino-group carrier protein]-C-terminal-gamma-(5-phospho-L-glutamyl)-L-glutamate + ADP</text>
            <dbReference type="Rhea" id="RHEA:52632"/>
            <dbReference type="Rhea" id="RHEA-COMP:13311"/>
            <dbReference type="Rhea" id="RHEA-COMP:13313"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136714"/>
            <dbReference type="ChEBI" id="CHEBI:136717"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 2/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the acetylglutamate kinase family. LysZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.17" evidence="9"/>
    <dbReference type="EC" id="2.7.2.19" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775686.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q0"/>
    <dbReference type="STRING" id="933801.Ahos_0884"/>
    <dbReference type="GeneID" id="10600355"/>
    <dbReference type="KEGG" id="aho:Ahos_0884"/>
    <dbReference type="eggNOG" id="arCOG00862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_2_0_2"/>
    <dbReference type="OrthoDB" id="6816at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00036"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043744">
        <property type="term" value="F:N2-acetyl-L-aminoadipate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04251">
        <property type="entry name" value="AAK_NAGK-UC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02082">
        <property type="entry name" value="LysZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037529">
        <property type="entry name" value="LysZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010662">
        <property type="entry name" value="PRK14058.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="35"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="262" mass="28305" checksum="66547FD17AEFACC9" modified="2011-06-28" version="1">MIVVKAGGRVIKNSLQNLIDSIISYNGKLIFVHGGGDIVTEYSKRMGIEPIFVTSPEGIRSRYTTREELDVYVMAMALINKNIVTALNTRGKLAIGISGADAAIVKANRKKRIMIIDERGKKRIIDGGYTGKIYEVNANLLNSISNVFDYIILSPIAIDISEGTMLNVDGDQMAFSVASAIKADTLVILTDVKGVLQNGEIVKKISKDEAEEFAKKVGAGMNRKILMSSEAVKNGVKKVIISSGLEKDAINKALNGEGTVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R5</accession>
    <name>F4B8R5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775701.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R5"/>
    <dbReference type="STRING" id="933801.Ahos_0899"/>
    <dbReference type="GeneID" id="71811285"/>
    <dbReference type="KEGG" id="aho:Ahos_0899"/>
    <dbReference type="eggNOG" id="arCOG04409">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165112_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="110" mass="12288" checksum="355CB87C66686443" modified="2011-06-28" version="1">MIRNGYKVLPPSFMVSLVKRAIKLSSLLPNVIETHPTSSMKNLGINWKEYAKKKDEIDAVICAITAYAYDNHIALKISANDGVIYLLPKGFPKPIKVSEGYYILPHIGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8R7</accession>
    <name>F4B8R7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alanyl-tRNA synthetase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0901</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775703.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8R7"/>
    <dbReference type="STRING" id="933801.Ahos_0901"/>
    <dbReference type="GeneID" id="10600373"/>
    <dbReference type="KEGG" id="aho:Ahos_0901"/>
    <dbReference type="eggNOG" id="arCOG01256">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656933_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051335">
        <property type="entry name" value="Alanyl-tRNA_Editing_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43462:SF1">
        <property type="entry name" value="ALANYL-TRNA EDITING PROTEIN AARSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Threonyl/alanyl tRNA synthetase SAD" evidence="4">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="150" mass="17529" checksum="21582857BCDF12FC" modified="2011-06-28" version="1">MREMIEIRTHSALHVLKGATRKVLGAKWTASTYVNGPHGRLTVQFERKPTEEEINRIFVEAQNKINEGLPFITEKLSREEAEKKYGDEIYDLFPVPPEVKELYIVIIPEWNINACNKQHVSNTKEIGEIKLDHWRYRNAKKLLEISFNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8U2</accession>
    <name>F4B8U2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von Willebrand factor type A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775728.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U2"/>
    <dbReference type="STRING" id="933801.Ahos_0926"/>
    <dbReference type="GeneID" id="10600398"/>
    <dbReference type="KEGG" id="aho:Ahos_0926"/>
    <dbReference type="eggNOG" id="arCOG02900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058577_0_0_2"/>
    <dbReference type="OrthoDB" id="33260at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040929">
        <property type="entry name" value="ArnB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18677">
        <property type="entry name" value="ArnB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="39"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="381" mass="42854" checksum="1ED69CF53CC64F59" modified="2011-06-28" version="1">MTLSLRVDMSHRYSFDNEVRMMFKILLVPEKISVATGFHYIILLDTSGSMEGLKIEKAKSGAIELFKRIPQGNKVTFIKFASTVEVVREFSDPLDLTNEIQNIAANGQTSLFTALLTAFKIAVKYSMPAYILLLTDGNPTDVTDTRTYEKMSIPQGVQIISFGIGDDYNEELLKILSDRTGSTFYHIQEASEIPEKLPRAAKTKIAGKNVTVDIISESPVKLLNYPQLPVKINAVEGVIKILGETVIPPKYNGNFMTIKVNYEDPVKNVQDVLMSVVSLKQAPDQQTFISGINKDIIMEYEYYVTLQKYSEDVNAGNLVEATRTLKKMEELAQQTRRLELIETTKRLSSDLETTKRIGSTEQTKRLSKEVSSEVTRKLRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8X3</accession>
    <name>F4B8X3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1404 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775759.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8X3"/>
    <dbReference type="STRING" id="933801.Ahos_0957"/>
    <dbReference type="GeneID" id="10600429"/>
    <dbReference type="KEGG" id="aho:Ahos_0957"/>
    <dbReference type="eggNOG" id="arCOG06032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109678_0_0_2"/>
    <dbReference type="OrthoDB" id="34641at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009844">
        <property type="entry name" value="DUF1404"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07185">
        <property type="entry name" value="DUF1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21158" checksum="0AF91F43AB498C3E" modified="2011-06-28" version="1">MQILRDKLTKKSLIIPVLFLIVAINPVTELLEPRYEALYMAMHYVLYIGGFVLGYIGFRGSKIYLIPGIIIPVFWHIPYFFNLGGAYLCLRIVEDLSLYLGGLVMGSTIHGLNNAIKAILFVLWMMGDSVLSVILIVGWPPYSNQVYPFSPFSISEEFWTGLVMFGIMTVIFIYIILGFIRTVFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Y3</accession>
    <name>F4B8Y3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Y3"/>
    <dbReference type="KEGG" id="aho:Ahos_2135"/>
    <dbReference type="eggNOG" id="arCOG07733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079288_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="117" mass="12928" checksum="A2700282FFAF0660" modified="2011-06-28" version="1">MMIKKKELVALLIAIVLISFTIYSYNLDHTIVFTKVTTQTKTETLPPGPGLGRDIDVPNATPLFCIFNGTVFHNGTATSIVYQTETTTIPPPFYATLWPVGLVALTGVLTYLLIRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B911</accession>
    <name>F4B911_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MoxR-like ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B911"/>
    <dbReference type="STRING" id="933801.Ahos_2163"/>
    <dbReference type="KEGG" id="aho:Ahos_2163"/>
    <dbReference type="eggNOG" id="arCOG00436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018678_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045427">
        <property type="entry name" value="MoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041538">
        <property type="entry name" value="RavA-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050513">
        <property type="entry name" value="RavA_ATPases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32204">
        <property type="entry name" value="ATPASE RAVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32204:SF0">
        <property type="entry name" value="ATPASE RAVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17868">
        <property type="entry name" value="AAA_lid_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20030">
        <property type="entry name" value="bpMoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="42"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="387" mass="44110" checksum="B583799E9FB39DFA" modified="2011-06-28" version="1">MYMGMQVDNKVLELPKKFLEALWSPFVGREEEAKVITLALLSKEHVVLIGEPGTAKSALARRAAELLNAKFFMYLLTKYTEPAELFGALDINALKQGVYKRITKDRLPESEIAFLDEIFNANSAILNALLSLLNERVIYDGYNVIKVPLRTLISASNRVPDEPELEALYDRLLLRHYAKPVGEEMWKNLIESAWELEFTTKWKVESPLMTIEDLDKIYSYLTEVDLSPVKSKLLKLYAMLEEKGIHLSDRRKGKVLKIISAHALLNGRLKATEEDLIVLKYIAPKEIDDFEKVSALLSEELKTPIKYMRELNEIYNNIKEAGKYVDAASESDPRLIDLIRSLKATRDRVIALGKESGDEKVEEFSKEVSAEIDKLLEKVGRKLGIYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B935</accession>
    <name>F4B935_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-deoxynucleotidase</fullName>
            <ecNumber evidence="5">3.1.3.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2187</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776973.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B935"/>
    <dbReference type="STRING" id="933801.Ahos_2187"/>
    <dbReference type="GeneID" id="10601690"/>
    <dbReference type="KEGG" id="aho:Ahos_2187"/>
    <dbReference type="eggNOG" id="arCOG04311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039453_4_2_2"/>
    <dbReference type="OrthoDB" id="46088at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD/PDEase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE95058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="180" mass="20526" checksum="40F39C1913708D42" modified="2011-06-28" version="1">MDLERMIVASKNLVRTGWMQKGIPPAIGETVADHSFEAAVIAYYLARKLGEKGINVNPDHAAVLALFHDAGESVLGDLPKWTTERINKKDAEKEAFDEFGFGKELFVEFKEQKTIESKIAKISDRLSTYLQSERYMKQGYEVKEIAESYLPEIENMISDYPITLIKDFIENVIRSKAFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B986</accession>
    <name>F4B986_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PFL family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775795.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B986"/>
    <dbReference type="STRING" id="933801.Ahos_0993"/>
    <dbReference type="GeneID" id="10600466"/>
    <dbReference type="KEGG" id="aho:Ahos_0993"/>
    <dbReference type="eggNOG" id="arCOG04321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048704_0_0_2"/>
    <dbReference type="OrthoDB" id="21376at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003700">
        <property type="entry name" value="PRK05313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560:SF1">
        <property type="entry name" value="UPF0210 PROTEIN MJ1665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="UPF0210 PROTEIN SPR0218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="445" mass="47429" checksum="954E6FED6396F09B" modified="2011-06-28" version="1">MKYSADEIIEVVRMLSEEDLDIRSVTLSVNTLTLISDDVNKTLGKFNSLNPILEKFSYSVDKVSEKYGVKIVTKRVAVSPVQYFLEVLAESDGIKIGEKLEELAEKNRIDYISGYSAFADRGFTKGSLSVINTLAEVMNSTRRLTGMINAASTFSGLNSDAIKKFVDQIFSMKPEASSRVSILSNVPPDSPFVPSAHHGIGMPDMMINVAVSGPGVIENAIKRSSPSSLEELHDVIKRSAFKISRLGELIGKAVSKEIGVPFGSVDLSLAPSPKVGDSVAGIIEAMGIEKIGGHGSLSALAILMDAIKKGGSMATSHVGGLSSAFIPVSEDSIMAERALEGYVDFFTLLALSSVCNSGIDMVGVSKKQGKDKVIGLIMDVLSLGITLNKILGVRVIPVDSEPGTVIDLGGLLGKVVVMRLKDVSVERFTSLNGFIPNTIKRLEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>F4B998</accession>
    <name>F4B998_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetate--CoA ligase</fullName>
            <ecNumber evidence="7">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1005</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the autotrophic CO(2) fixation pathway. Activates 3-hydroxypropionate to its CoA ester. Can also activate propionate, and to a lesser extent acrylate, acetate and butyrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxypropanoate + ATP + CoA = 3-hydroxypropanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26534"/>
            <dbReference type="ChEBI" id="CHEBI:16510"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.36"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775807.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B998"/>
    <dbReference type="STRING" id="933801.Ahos_1005"/>
    <dbReference type="GeneID" id="10600478"/>
    <dbReference type="KEGG" id="aho:Ahos_1005"/>
    <dbReference type="eggNOG" id="arCOG01529">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_2"/>
    <dbReference type="OrthoDB" id="371752at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043955">
        <property type="term" value="F:3-hydroxypropionyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043427">
        <property type="term" value="P:carbon fixation by 3-hydroxypropionate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF232">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="16"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="71"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="519"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR02188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="646" mass="73618" checksum="1C7BCE3097A5F2CF" modified="2011-06-28" version="1">MSENLPFEERYYQPPYKQLYKQSLEEPEKFWREMAEGLEWFSPFDKVLDESNAPFYRWFVGGKINITYNALDRQVKVRRNKIAIYWENEKGESRQLTYGELYNEVNRFAKILQDLGIKKGDRVLIYLPLMPELPIAMLACARIGAVHSVVFSGFSTQAVIDRINDAKAKLVITTSHTMRRGKRVELKRIIDEAVSQTPSVEKVLVIKRDEEIELNEERDVLYQDLIKRTGYKKVEPEETLSTDPLFILYTSGTTGKPKGILHLHGGYGLWTYLTTQWVFDLRDTDVFFNTADIGWITGHSYIVYGPLQNGASVLMYEGVPDYPNPDRWWELVEKYGVTVFYTSPTAIRSLMKYGEEWVKKHDLSTLRILGSVGEPINPEAWRWYFKNVGNSSLPIVDTWWQTETGGIMISATPGLGNYLLKPSANGLPLPGVDADVFTEDGKQAKPREKGYIVIKRPWPGMLAGVWGDPERYVKTYFSKFPGVYYPGDYAVRDEEGFFYILGRADEVLKIAGHRIGTREIEDILISHPAVAESAVIGVPDPVRGEVAVAAIVLKQGYQPSEELRKSLIEYVKNNLGPIVIFGGLYFVSKLPKTRSGKIMRRVVRSVISGQPLGDVSTLEDETSVDELKKVIEEFTKEIQGQQNEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B1</accession>
    <name>F4B9B1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ion transport 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775820.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9B1"/>
    <dbReference type="STRING" id="933801.Ahos_1018"/>
    <dbReference type="GeneID" id="10600493"/>
    <dbReference type="KEGG" id="aho:Ahos_1018"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109464_0_0_2"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF9">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN YUGO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="4">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="117"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="250" mass="27572" checksum="C991C117AEA92502" modified="2011-06-28" version="1">MLLSVRSIASLLLLGGVVIFGVAGSFILGHLGHNFNECMNLINSIYFTVVTLSTVGYGDIVPITPIGKIFVVILIVFGMGAFLTALTSISGDIASKRILDLTSKLEKIEEEMSKVKVLLIGGGGVNISLAENFEKEKLKYILLLSDKTEAEKLSEKGIKAYAINLISEDEIRKFHPEKVKSIIVDMQNSSDMLYVILILTELAKESNIITIVHNEDLEKRLESVKKIKDIKIINPSKQVANELLSLLREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B5</accession>
    <name>F4B9B5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ordered locus">Ahos_1022</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9B5"/>
    <dbReference type="STRING" id="933801.Ahos_1022"/>
    <dbReference type="KEGG" id="aho:Ahos_1022"/>
    <dbReference type="eggNOG" id="arCOG01432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042858_1_0_2"/>
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        <property type="reaction ID" value="UER00044"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:pyridoxal phosphate binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Trp_synth_b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
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        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK12391.1"/>
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    </dbReference>
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        <property type="entry name" value="trpB_rel"/>
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    </dbReference>
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        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="81"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="435" mass="48374" checksum="16DE7E82B2FBEB7C" modified="2011-06-28" version="1">MTSSFLLTMIVRYDLPQDKIPTTWYNILPDLPEPLPPPKDETGKAFEVLKEVLPSKVLEIEFSQERYIKIPEEVLEKYLQIGRPTPIIRARKLEEYFGNVVKIYMKMESHTYTGSHKINSALAHVYYATLDNAKFVSTETGAGQWGAAVALAAALFHIKAHVFMVRTSYFAKPYRRYLMQMYGADVHPSPSEFTPYGREVLQKDPNNPGSLGIAITEAVKYALENGGRYVVGSVVNSDILFKTIAGQEAKVQMEMIGEDPDYIIGVVGGGSNYAALAFPFLGEELRSGKVRRKYIASGSLEVPKMTKGVYKYDYPDTGKILPQLKMYTIGSDFIPPPVYAGGLRYHAVAPTLSLLMSKGIVEARDYDQETAFAWAKLFSQIEGWVPAPETSHALPIIKEIADEAKKSGEKKTVLVSFSGHGLLDLANYAEVLGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9B8</accession>
    <name>F4B9B8_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Ahos_1025</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="AEE93911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="57" mass="6611" checksum="B444A48381A80C5F" modified="2011-06-28" version="1">MSIITASSLFIAYIDYIEVIKIFIAKNKNLNGKRKNVEYMKFCRKLVPHYLKPSTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9F4</accession>
    <name>F4B9F4_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9F4"/>
    <dbReference type="STRING" id="933801.Ahos_2229"/>
    <dbReference type="GeneID" id="10601734"/>
    <dbReference type="KEGG" id="aho:Ahos_2229"/>
    <dbReference type="eggNOG" id="arCOG08319">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2597913_0_0_2"/>
    <dbReference type="OrthoDB" id="32958at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="79" mass="8796" checksum="C96E78409129B2F6" modified="2011-06-28" version="1">MKELDLTKQSGGCGANTPSVSLMKFWLETGGNQEVKIIALQGVQADQVEMWAMAMQEKGVKILNKEFSEDKVIYTVYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K2</accession>
    <name>F4B9K2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase zinc-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2277</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777063.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9K2"/>
    <dbReference type="STRING" id="933801.Ahos_2277"/>
    <dbReference type="GeneID" id="10601783"/>
    <dbReference type="KEGG" id="aho:Ahos_2277"/>
    <dbReference type="eggNOG" id="arCOG01456">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_1_2"/>
    <dbReference type="OrthoDB" id="73567at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350:SF2">
        <property type="entry name" value="GROES-LIKE ZINC-BINDING ALCOHOL DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43350">
        <property type="entry name" value="NAD-DEPENDENT ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="14"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="362" mass="38359" checksum="87794C63944CD407" modified="2011-06-28" version="1">MKGAVLYKYNEPLKIEDNLQIQSPKEGEVKVKIVATGMCHSDINVFEGKTPVPPPVIAGHEIAGIVEEVGPGVTRVKPGDRVVSAFIHPCGKCRNCISGHENLCETFSAVRLKGTMMDGTTRVRLDGKEVRTFLGGGFAEYAVVGENALTVVPPEVDLEKIAVLGCAGLTGYGAVNSAKIEPGESVAVIGVGGVGLSVIQLLKASGAGRIIAVGTKKWKLDRAIELGATDVVNSKETDPIKAIKELTNGGADVVIEAGGNEETIKMAMDSVRIGGRVVLVGLPPVNAMIPFRVASIVRNGITIIGNYGGRPRIDMPRLLDLVRLGKYDPSKLITGRFKLEEINEAVKLLNQGEAIRSLIIPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9L9</accession>
    <name>F4B9L9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9L9"/>
    <dbReference type="STRING" id="933801.Ahos_1051"/>
    <dbReference type="KEGG" id="aho:Ahos_1051"/>
    <dbReference type="eggNOG" id="arCOG01389">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023978_2_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06423">
        <property type="entry name" value="CESA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF1">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="393" mass="44786" checksum="3D8B2BB9CF430545" modified="2011-06-28" version="1">MLYVIVILSSLVSAWSVYNSFLAIVGIRWNPKEYKNPSGVSFSLIIPAKNEEKVLGRLLDRLENQEYDRSKYEIIVVEDGSTDKTLEVCNSYKLMYDNISCIHLESSRVINGKSRALNYALRIAKGEIIGIFDADTVPRLDTLAYASAKFEDPKVAGVQGRLVPINVRESAIARFASLEELFYEYSISGRARLGFFVPLEGTCSFIRKSVLESLGGWNESSLTEDLDLSLKIISSGYKIIYSPSIVAWREVPVSLRMLIKQRLRWYRGHFEVTLKVEKVKFDWRVIDAILIVATPVFMILNLVNYSLVLLYPSEIYIVVVSLVSFASLLSLLLGIMISRKHMIEEFYPILSFIYMNLVVILNLIAISLELLRMPKKWIKTERTGNITVRIHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M9</accession>
    <name>F4B9M9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase/mu-crystallin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775861.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M9"/>
    <dbReference type="STRING" id="933801.Ahos_1061"/>
    <dbReference type="GeneID" id="10600535"/>
    <dbReference type="KEGG" id="aho:Ahos_1061"/>
    <dbReference type="eggNOG" id="arCOG01035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_2_1_2"/>
    <dbReference type="OrthoDB" id="21421at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="313" mass="34264" checksum="4411C67578297407" modified="2011-06-28" version="1">MALLLTEKDVSNLLKFEDAYVALKEAFIALESKAGVNSKRMRTSISGSTLTCQAGGLQGYLGIKTFIKGNFVSLLFSTSGELLMIAEADRLSQIRTGSLSVLASDYIQIKYDTVGIIGLGKQGLAQVEAFYELKKIEPIVISRTQERINKALRILNSEGIKVKVAGSYKDVFLNSEVITSITSSKDPFIKLDYVRKGMHINLMGSNIPERVEAFPELIKASSIIVVEDIEQALEEAGDLILAKKMGMLDESKLVTLSSVIAGKVEKKKDDDISIFKSTGVGLEDVAVMKVLYEKAKKQGIGKEIEVKGVWSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9N1</accession>
    <name>F4B9N1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1063</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9N1"/>
    <dbReference type="STRING" id="933801.Ahos_1063"/>
    <dbReference type="KEGG" id="aho:Ahos_1063"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_13_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="195" mass="22033" checksum="5BB43FD9D90D77AB" modified="2011-06-28" version="1">MDDNSQDGTAEAVRSLNSPNIQVIVREKERGLGSAIRTGIQKAIENGTDYVVTMDADLSHDPIYLPAMYEKAREGFDLVIGSRYVKGGGIENWPIKRRIISWGANFLVRLLLRSPLHDNTSNYRIYSTRAAKEVLKCESADGYEFQICAVYRVLKANLPVAEVPIIFKDREIGKSKLTIGQIYKWFKYVISLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9R3</accession>
    <name>F4B9R3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hydroxybutyryl-CoA dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_1095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775895.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9R3"/>
    <dbReference type="STRING" id="933801.Ahos_1095"/>
    <dbReference type="GeneID" id="10600569"/>
    <dbReference type="KEGG" id="aho:Ahos_1095"/>
    <dbReference type="eggNOG" id="arCOG00239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_2"/>
    <dbReference type="OrthoDB" id="27846at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000009">
        <property type="entry name" value="Carnitinyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="257" mass="28091" checksum="8F74E8BD22BF6998" modified="2011-06-28" version="1">METVETKIENGIGWIILNRPDKLNAINLKMLEELEEVTKNFEENNDVKIIIFTGNGKAFSAGADISQFKELNSISAWNFAKKGRRVMDYIESVSKPTIAMINGYALGGGLELALACDFRIAAEEASLGLPEINLGIYPGFGGTQRLVRAIGKAKAMELMMTGDRISAKEAERIGLVNKVVSLSSLKEETIKFAGKLMEKSPIALAILKHIILYGNDSPLLDGLNMESLGWGVAFSTEDEKEGVSAFLEKRKAVFKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9S5</accession>
    <name>F4B9S5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbamoyl phosphate synthase small chain</fullName>
            <ecNumber evidence="8">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Carbamoyl phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">carA</name>
        <name evidence="10" type="ordered locus">Ahos_1107</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775907.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9S5"/>
    <dbReference type="STRING" id="933801.Ahos_1107"/>
    <dbReference type="MEROPS" id="C26.952"/>
    <dbReference type="GeneID" id="10600581"/>
    <dbReference type="KEGG" id="aho:Ahos_1107"/>
    <dbReference type="eggNOG" id="arCOG00064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_2"/>
    <dbReference type="OrthoDB" id="7675at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="370" mass="41360" checksum="A9092DA9BE227015" modified="2011-06-28" version="1">MICKRGYKGYIYLEDGTLIEGCGFGAKGIKAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGIPKPIVENGILRNFESERIQVEGLVVAEETDPYKWNSQMSLHDWLKSEGVPGVSDVDTRMLVKKVRNKGVMMGVISSGIELDDPKKYLEKKYDEIDFTQFTSPKSPIIHQGNGKGIIVLVDCGIKHGILYQLNSRGYTVVRVPCNFTANQIMDYSPQGVVFGNGPGNPKLLQGLIKTYKDIVEYKVPIMGICLGHQITSLALGGNVRKMKYGHRAINKPVIDVTDNKCYITTHNHGYGVFKEDVPKDMKVWFYNPDDGVVEGMIHNKLPIITTQFHPEARPGPWDVTWVFDKFKKVIESGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Y6</accession>
    <name>F4B9Y6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2331</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777117.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Y6"/>
    <dbReference type="STRING" id="933801.Ahos_2331"/>
    <dbReference type="GeneID" id="10601839"/>
    <dbReference type="KEGG" id="aho:Ahos_2331"/>
    <dbReference type="eggNOG" id="arCOG00910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074702_1_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE95202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="153" mass="17341" checksum="069C208F3880973A" modified="2011-06-28" version="1">MADLGCGTGRFCAAASFLGAYCACIEIDNESITLAKKVFKELGIDADFINADVEYFHSQSKFDTIIENPPFGVKRKGYDLIFLNSALDLSNDVVYSIHKSNEKSEEIIKNIARKKGFSSEVITTNFEIGYYYPWHKNNIHKFLVDIYLFKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9Z1</accession>
    <name>F4B9Z1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2336</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777122.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9Z1"/>
    <dbReference type="STRING" id="933801.Ahos_2336"/>
    <dbReference type="GeneID" id="10601844"/>
    <dbReference type="KEGG" id="aho:Ahos_2336"/>
    <dbReference type="eggNOG" id="arCOG03119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369584_0_0_2"/>
    <dbReference type="OrthoDB" id="34468at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="201" mass="22363" checksum="70D7DDAA549EB6F4" modified="2011-06-28" version="1">MRIFYLIIILSISIVTNATPFFGTPYTLVATTLLLKCGITPINLAEAIILTGIGAAVAKSFMYFIGLGARKGLKRNKNVIFFSRFTGKKSFYIILFLTSIFPFLPLDDLVYLIGGTTKTSLISMLKISLLAKIIKSSIEIPIEAFGILQISEAIGISPFETGVISTVVMTILAIILFKIDWESIYRKIEKYLANNKYFRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA03</accession>
    <name>F4BA03_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">malonyl CoA reductase (malonate semialdehyde-forming)</fullName>
            <ecNumber evidence="5">1.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2348</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>3-oxopropanoate + NADP(+) + CoA = malonyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26446"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.75"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.75" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777134.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA03"/>
    <dbReference type="STRING" id="933801.Ahos_2348"/>
    <dbReference type="GeneID" id="10601856"/>
    <dbReference type="KEGG" id="aho:Ahos_2348"/>
    <dbReference type="eggNOG" id="arCOG00494">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="OrthoDB" id="38238at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23940">
        <property type="entry name" value="ASADH_C_MCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000411">
        <property type="entry name" value="Aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00052213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="355" mass="39228" checksum="DC2FDFB4BD3945D8" modified="2011-06-28" version="1">MRRILKAAILGATGLVGIEYVRMLSQHPYIKPAYLAGKGSVGKPYSEVVRWQTIGQVPKEVADMEVKPTDPKLMDDVDIVFSPLPQGAAGPIEEQFAKEGFPVISNSPDHRFDPDVPMLIPELNSHTISLIDEQRKKRDWKGFIVTTPLCTAQGAAIPLAPIFMNFKMTGAYITTIQSLSGAGYPGIPSLDVVDNILPLGDKYDAKTIKEITRILSEVKRNVNDPPLEEVALDATTHRIATVHGHYEVSYVTFKEDVNVEKIRETLENFRGEPQDLKLPTAPEKPIIVRNEDTRPQVYFDRWAGDPPGMSVVVGRLKQISNRTIRMVSLIHNTIRGAAGGGILTAELLIAKGYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3K9</accession>
    <name>F4B3K9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative pterin-4-alpha-carbinolamine dehydratase</fullName>
            <shortName evidence="4">PHS</shortName>
            <ecNumber evidence="4">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pterin carbinolamine dehydratase</fullName>
            <shortName evidence="4">PCD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0003</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774815.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3K9"/>
    <dbReference type="STRING" id="933801.Ahos_0003"/>
    <dbReference type="GeneID" id="10599443"/>
    <dbReference type="KEGG" id="aho:Ahos_0003"/>
    <dbReference type="eggNOG" id="arCOG02939">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081974_4_5_2"/>
    <dbReference type="OrthoDB" id="10495at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00488">
        <property type="entry name" value="PCD_DCoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002017">
        <property type="entry name" value="PRK00823.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE92898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="96" mass="11330" checksum="48E22C78638BECA3" modified="2011-06-28" version="1">MNKLSEDEIRDKLKNLNNWEYKDNKLVKEFKFNTFDDSVNFLKLIQPIADSLDHHPDVHIYYNRVIIELTTHDAGGITDLDFKLAEKIDDLSTHVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3N3</accession>
    <name>F4B3N3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774839.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3N3"/>
    <dbReference type="STRING" id="933801.Ahos_0027"/>
    <dbReference type="GeneID" id="10599467"/>
    <dbReference type="KEGG" id="aho:Ahos_0027"/>
    <dbReference type="eggNOG" id="arCOG08073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1242968_0_0_2"/>
    <dbReference type="OrthoDB" id="42456at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="222" mass="26174" checksum="0563C8A341C9DD5B" modified="2011-06-28" version="1">MNLVDILLKIQNEKNSLDWEKLKKEYMEQGEIIKSLEVTVSKIHSIKQELRRCSLNEVSEEYLAIKNYLSKAKTSDNPREIISYVNNAYEELKHCLKLSEDIIKEKIQKYKEIIDENNRKLKTYLKIFLTILGESKDLRLFEITDNLEELERNAKESEEEARKIYEELKDKLSKLNIEGKRLEILLSLLDQGQVTITKRNSKDVIELLRFLSEKGIIITVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P3</accession>
    <name>F4B3P3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0037</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774849.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P3"/>
    <dbReference type="STRING" id="933801.Ahos_0037"/>
    <dbReference type="GeneID" id="10599477"/>
    <dbReference type="KEGG" id="aho:Ahos_0037"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000589">
        <property type="entry name" value="ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050763">
        <property type="entry name" value="ABC_transporter_ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42711:SF5">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE92932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="240" mass="27146" checksum="A98E8A1EEE48EFDF" modified="2011-06-28" version="1">MITISVEDLWKSYKDKEVLKGISFTVNDGEIFSLLGPNGAGKTTTIKILSCVSKPDRGKIEILGMKVPEDCEKIRRIIGIVPQDFQGFSDLTVKDNIEYFANIYGGDKSQVNEVIEELDLEDYKNTRFKKLSGGLKRRVAIACALVGNPRIIYLDEPTVGLDPKARRNVWDIMKKLKDKKLTILLTTHYLDEAQKLSDRIAIIYSGKILRLSTPDDLMKEFEKPTLEEAYLALLESISEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3Q6</accession>
    <name>F4B3Q6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="4">3-dehydroquinase</shortName>
            <ecNumber evidence="4">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Type I dehydroquinase</fullName>
            <shortName evidence="4">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aroD</name>
        <name evidence="5" type="ordered locus">Ahos_1210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776009.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3Q6"/>
    <dbReference type="STRING" id="933801.Ahos_1210"/>
    <dbReference type="GeneID" id="10600686"/>
    <dbReference type="KEGG" id="aho:Ahos_1210"/>
    <dbReference type="eggNOG" id="arCOG02097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064444_2_0_2"/>
    <dbReference type="OrthoDB" id="34329at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050146">
        <property type="entry name" value="Type-I_3-dehydroquinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43699:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="4">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="30"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="210" mass="24119" checksum="DF9B3C24FFF40B86" modified="2011-06-28" version="1">MRPLIVASLPIYKEDDLLKARNIKEADMIELRLDYSPKLIKLEKIKEILGDLKEKLILTIRDVNEGGVYKITDSEKAKYLQEANVEGFIYDVEASFLERFDVSFKGKIVSSHYFNELPKYEEVERIIKKYGNDALFIKIATIGKGEYKELLTKLLKFDKIVVLPMGVDPLERIALGILGSRLIYTFVEAQTAPGQMHYSKAFKIISCLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U0</accession>
    <name>F4B3U0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA (guanine(10)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="12">2.1.1.213</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA:G10 dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Ahos_1244</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10358"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.213"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the methyltransferase superfamily. Trm-G10 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.213" evidence="12"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776043.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U0"/>
    <dbReference type="STRING" id="933801.Ahos_1244"/>
    <dbReference type="GeneID" id="10600723"/>
    <dbReference type="KEGG" id="aho:Ahos_1244"/>
    <dbReference type="eggNOG" id="arCOG00047">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057819_0_0_2"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160101">
        <property type="term" value="F:tRNA (guanine(10)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000251">
        <property type="entry name" value="Putative RNA methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF13">
        <property type="entry name" value="TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="14">
        <location>
            <begin position="138"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE94127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="310" mass="34701" checksum="6D3F458446E4BDC8" modified="2011-06-28" version="1">MKYAILSADELFISLAELKALLKRPFSYVTGIAIFDGEAKNIARRSSTIKAIGEILAISDNPKEINDALKGKCFSIKPNVIMNSDKDLFPVLYKEIVDGVKFSKKCDKLDLIFTDGVILAGIRQEERDSKSLQAHAKKPYSQSGTMDAYTSRLLVNLANPEKTILDPFAGVGSILIEASWLGYNCIGGDIDSKMIEKTKYNLKHFNYECQIIQEDISNLPIKENSVDAIVTDPPYGRSVNARDVKFDELYESLFFNSAEVLKKGGKLVFATDSKFDWRDKIKSSGLKVDSIHFIYLHKSLSRGIYVVEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3V2</accession>
    <name>F4B3V2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type sulfate transport permease, CysU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1256</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776055.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3V2"/>
    <dbReference type="STRING" id="933801.Ahos_1256"/>
    <dbReference type="GeneID" id="10600737"/>
    <dbReference type="KEGG" id="aho:Ahos_1256"/>
    <dbReference type="eggNOG" id="arCOG00164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="OrthoDB" id="11163at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="SULFATE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF8">
        <property type="entry name" value="SULFATE TRANSPORT SYSTEM PERMEASE PROTEIN CYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="44"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="254" mass="28064" checksum="61890AF648E87588" modified="2011-06-28" version="1">MNWFRLLGIFLSIILAVPVLFLIYMGFFVFKSPQAFSQAFYSGVELSVLSSASAAGIDFALFTPLAYYLSREKDFITDSLVDVPASIPHPIVGVALVFLDSPYSPFYKIFQDLGINFFDTYYGLISALVIVSAPIYIRSMKNYFDSMPRVYEEYARSLGAGMGRVYLMVIKNSLKGALSSSLTSMSRAMSEFGSIAIVAYYVLYGPLAGTSPASILIYEYYGYYGPQVAVTASAIMIVVGIILLISLKLIQYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W9</accession>
    <name>F4B3W9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776072.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W9"/>
    <dbReference type="STRING" id="933801.Ahos_1273"/>
    <dbReference type="GeneID" id="10600754"/>
    <dbReference type="KEGG" id="aho:Ahos_1273"/>
    <dbReference type="eggNOG" id="arCOG01804">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053800_0_0_2"/>
    <dbReference type="OrthoDB" id="10037at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055767">
        <property type="entry name" value="DUF7343"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014466">
        <property type="entry name" value="Txn_rg_UCP012876"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024:SF42">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24034">
        <property type="entry name" value="DUF7343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012876">
        <property type="entry name" value="Txn_rg_UCP012876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7343" evidence="1">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="299" mass="33961" checksum="90BDD9D512F35778" modified="2011-06-28" version="1">MKGRDRILSILKERGSLPQTELVKISGMSKSRVSEILNELEKEGIIFRKQLVGKNLIVSLNETKFLRLGIINAAEYPFIIPFIKKLREKGIEVEVKIYDNGLQVTKDLVLGKIDMGFSPVISQIIFSKIFDIKIIAGGAKGGAGIVGESCNIGSTVMSSMETWTLAEVKNANIIPFNSPQELVKNFETKKVPAIAIWEPYLSILESKGHKVTHVFEPNHCCTLAIRSSLEDEEKIKEIYEDAFSWFLSSKDRWISDYSNLLGEDYNIMKKASERYYFDSYLDLKEVYKNLKKMNIYIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B420</accession>
    <name>F4B420_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774901.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B420"/>
    <dbReference type="STRING" id="933801.Ahos_0091"/>
    <dbReference type="GeneID" id="10599531"/>
    <dbReference type="KEGG" id="aho:Ahos_0091"/>
    <dbReference type="HOGENOM" id="CLU_2406174_0_0_2"/>
    <dbReference type="OrthoDB" id="41358at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE92984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="92" mass="10494" checksum="E87B36B613721569" modified="2011-06-28" version="1">MKYQELISIKNDLFISKNAADMIMYMKPGEKIVFKKNQELSLIVHKGEGELSIGKRRVKLKEKSTIISKESTEKIIVAITSMLLFIHIEAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B431</accession>
    <name>F4B431_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome partitioning ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774912.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B431"/>
    <dbReference type="STRING" id="933801.Ahos_0102"/>
    <dbReference type="GeneID" id="10599542"/>
    <dbReference type="KEGG" id="aho:Ahos_0102"/>
    <dbReference type="eggNOG" id="arCOG07293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090891_1_0_2"/>
    <dbReference type="OrthoDB" id="36110at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE92995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="254" mass="29181" checksum="FD07E1A54DD923EB" modified="2011-06-28" version="1">MRLSVLSTKGGVGKSTIALLLSKYFSLNGVKTLLIDRDPLGWVSNLAKIKGKGLLASIADKEEDKQAYFKEVKTKDGGDFYILKLYGDGARFYVDLDIIRRDEKLYKKIKEKYQEIVRNGFQAFIVDNPSMVQWNDDEVQCELRIFRETFPDEKIFRLYISDSSEISIESTLKYIKEIEQTPHKIGQYLGIVINLVPPFPEDLDKAMRLASKFEGIKVVIPFIESLFILNDINVEIPEQVKYLGKEILKLNNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B458</accession>
    <name>F4B458_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphate uptake regulator, PhoU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776082.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B458"/>
    <dbReference type="STRING" id="933801.Ahos_1283"/>
    <dbReference type="GeneID" id="10600764"/>
    <dbReference type="KEGG" id="aho:Ahos_1283"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069302_1_0_2"/>
    <dbReference type="OrthoDB" id="40991at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF2">
        <property type="entry name" value="PHOU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="349" mass="39923" checksum="FF3937DEB8D7C576" modified="2011-06-28" version="1">MEVRRVQKFGKSTLMVSLPADWVKEVGLNPGESIYLEVDEDGSLKVYPPNLKTEGSSKEMKIEIKTNVSPELISRIIYSLYILGFDKITIESTDGPFNEDILRKVKDTVRSLIGLEIVSQDLTSLQIQSFLDPTKYSMASLVSRLSNTLKQMLHYLNLGIKEASRTFLQEVLELEKEVDRLYYLSLRQLLIAQVNRSLAYMIGVKRIQIIGNRILMKAIEEAADEISEAASDLLSLHPEDLEVLKVSWDKIDMIVDQTAVVVDHVVKVLNKEDLKLVNEVLEELRTLRRVLMTEALMAEEKIEKNNSTRVTVVFRSLNLRLYNAIRRMEPIAEIAFNRSIENLKEIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B468</accession>
    <name>F4B468_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secE</name>
        <name evidence="10" type="ordered locus">Ahos_1293</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776092.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B468"/>
    <dbReference type="STRING" id="933801.Ahos_1293"/>
    <dbReference type="GeneID" id="10600774"/>
    <dbReference type="KEGG" id="aho:Ahos_1293"/>
    <dbReference type="eggNOG" id="arCOG02204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191921_1_0_2"/>
    <dbReference type="OrthoDB" id="37044at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="58" mass="6750" checksum="1B47F5E13E6340B3" modified="2011-06-28" version="1">MNLLERIKKLPDDWKRIITVAKKPDKDAFSMYLKVTLIVMAFIGLLAYLIQLILAFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>F4B476</accession>
    <name>F4B476_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Arginine decarboxylase proenzyme</fullName>
            <shortName evidence="9">ADC</shortName>
            <shortName evidence="9">ArgDC</shortName>
            <ecNumber evidence="9">4.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pyruvoyl-dependent arginine decarboxylase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="9">Arginine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="9">Arginine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_1301</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Specifically catalyzes the decarboxylation of L-arginine to agmatine. Has no S-adenosylmethionine decarboxylase (AdoMetDC) activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="9">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amine and polyamine biosynthesis; agmatine biosynthesis; agmatine from L-arginine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterooctamer of four alpha and four beta chains arranged as a tetramer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776100.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B476"/>
    <dbReference type="STRING" id="933801.Ahos_1301"/>
    <dbReference type="GeneID" id="10600783"/>
    <dbReference type="KEGG" id="aho:Ahos_1301"/>
    <dbReference type="eggNOG" id="arCOG00279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_1_2"/>
    <dbReference type="OrthoDB" id="114016at2157"/>
    <dbReference type="UniPathway" id="UPA00186">
        <property type="reaction ID" value="UER00284"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.90.10:FF:000005">
        <property type="entry name" value="Arginine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.90.10">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01298">
        <property type="entry name" value="ArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027549">
        <property type="entry name" value="ArgDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866:SF2">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="1 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 9" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0704">Schiff base</keyword>
    <keyword evidence="4 9" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023346002" description="Arginine decarboxylase beta chain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023346003" description="Arginine decarboxylase alpha chain" evidence="9">
        <location>
            <begin position="76"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="9">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="9">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="9">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="9">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="9">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="128" mass="14607" checksum="4FD544B9D8CE8A84" modified="2011-06-28" version="1">MEERLVLKSSEDKIVGKHVFGNLYDIDPKLLTDREYLEKLVLEAVKIANMKLVEAKSWSFGGKKGGVSVLALVEESHIALHTWNEYNYATLDVYTCGEQSDPQAAFDYIVKALKPRRYQVFYADRSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4B3</accession>
    <name>F4B4B3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine-pyruvate transaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1338</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776136.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4B3"/>
    <dbReference type="STRING" id="933801.Ahos_1338"/>
    <dbReference type="GeneID" id="10600824"/>
    <dbReference type="KEGG" id="aho:Ahos_1338"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_2"/>
    <dbReference type="OrthoDB" id="35685at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF39">
        <property type="entry name" value="SOLUBLE HYDROGENASE, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="18"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="372" mass="41104" checksum="4E038CB3C76AB8B7" modified="2011-06-28" version="1">MILIPGPVNVPQSVSFEATRVVNHRSDEFREVVKSLEEKMIKHFDSSRVALLTGSGTLAVESMVFSLLKRGEKVIVFTYGEFSNRLLDSINRRGCTTKVYSKPAGEGFSLEDVKVALDENKDADAVALVHNETSTGIAFRNLREVAKLVKNSGKKFIVDSVSGFAAYEIDVNNWHIDAIATGSQKALASVPGMGFVAMSEEGVSELHDDDMPNYLNLKLALKFQDKHETPFTPSTGAFFASLRAAEILEMEGLKNRIKRHEACSRYLRSVLSSISFSLFGNESNFSNTVVAGIPPIPSKTLREELKKRGIEISGGMGELRDKIIRIGILGVVDDRAISRLLLSLSDVLKQDIEIKPPADCKLPEFLRQEVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4C6</accession>
    <name>F4B4C6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">5-formyltetrahydrofolate cyclo-ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0122</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="933801.Ahos_0122"/>
    <dbReference type="KEGG" id="aho:Ahos_0122"/>
    <dbReference type="eggNOG" id="arCOG00474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031500_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017:SF0">
        <property type="entry name" value="METHENYLTETRAHYDROFOLATE SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="221" mass="24926" checksum="91F737A6BB6E3F25" modified="2011-06-28" version="1">MEETNIASFPRPVFGRIPNFKGSEKAAELLSTLPEFKKAEVVKVNPDSPQRKVRELVLSAGKKLLVPTPRLKGEFFLLYNVNPKLASTISGFTKLGKRISYKEIPPVDFIVVGSVAITRSGDRVGKGEGYSELEYAILRELGKVNEKTPVATTVHSIQIVDEIPSQPFDVPVDIIVTEKEIIRTKPNREKPKGIYMEYLTKEKIEETPYLKYYLMETGRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4D6</accession>
    <name>F4B4D6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Conserved transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0132</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774942.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4D6"/>
    <dbReference type="STRING" id="933801.Ahos_0132"/>
    <dbReference type="GeneID" id="10599572"/>
    <dbReference type="KEGG" id="aho:Ahos_0132"/>
    <dbReference type="eggNOG" id="arCOG00271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978689_0_0_2"/>
    <dbReference type="OrthoDB" id="34518at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050638">
        <property type="entry name" value="AA-Vitamin_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32322:SF18">
        <property type="entry name" value="S-ADENOSYLMETHIONINE_S-ADENOSYLHOMOCYSTEINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="7"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="136"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="283" mass="31780" checksum="70D09DB92F560575" modified="2011-06-28" version="1">MVKLYYIIPYVLINSLIYVFVKDGLYYASPMFFMALRFLIGGIILLPFAKQLTLNRDIFLLSIFTTLSTSFWAYGLLYVEPSESAVLSYTMPLIAIPLSALILREKTTKTEVIGILIGFSGVVIYSLNLGIYFSLIGIVLTLINAFFWALFTVYFRKLRGFDATSVNAVQLLLGSLIFFTLSPIQFYFKYSINFLVDLLYVSVLGGGISFYLWNSMLKTERVSKVTVLSFSVPAVSTAVDELRGVNVNIGMIEGIGVMFLGILISRLEKKINKSNIVNGRFHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4H4</accession>
    <name>F4B4H4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4H4"/>
    <dbReference type="STRING" id="933801.Ahos_0171"/>
    <dbReference type="KEGG" id="aho:Ahos_0171"/>
    <dbReference type="eggNOG" id="arCOG07231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_724832_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="374" mass="41071" checksum="B40914C4E9E29700" modified="2011-06-28" version="1">MGVSLFLIILLIVVVSIYHNLTQVSQYSILYISNFSKIEDIIALNASTLFIVGSKTCGNISHGVAGYYYLNDNEFKQVNLSKLFCCGAIYSVAYNGSALMLAGACKISGVLHPAIALISYNKVTNLTSYIPMGYYPGRALAVAWFNKTWFIGGNFLFNLDQHYVSVMFLIGLKGNSTSNLTLNISNILNQLPEGCILSLSVGHNCILIGGKFVIYLVLFKFNSTSIYEINMTKSIFKYPGFIISLSQFNSSTWVVGGEYFPVNSTICHPYLVLYNSLNSCINYIKLNYTIGFVTSVSSYNGNIVVSLRVPFKTPQGVEGGTVILEGKINALKQTFDEVNISINQLLSLNNRIIGGGYKVTGNADEGILIFLNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4R7</accession>
    <name>F4B4R7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alba, DNA/RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4R7"/>
    <dbReference type="STRING" id="933801.Ahos_1414"/>
    <dbReference type="KEGG" id="aho:Ahos_1414"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_2_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="88" mass="10209" checksum="66D6119C3EBF4C7D" modified="2011-06-28" version="1">MNKPFEVVVSRSKSVEDHVLEIIEILNHNSNEVDLKGYGFEINKAVDIYNALKDRLKEGITLEEVSIGSETKDRKRISYLLLRIKRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4S6</accession>
    <name>F4B4S6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="10">SRP54</shortName>
            <ecNumber evidence="10">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srp54</name>
        <name evidence="15" type="ordered locus">Ahos_1423</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776221.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4S6"/>
    <dbReference type="STRING" id="933801.Ahos_1423"/>
    <dbReference type="GeneID" id="10600915"/>
    <dbReference type="KEGG" id="aho:Ahos_1423"/>
    <dbReference type="eggNOG" id="arCOG01228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="OrthoDB" id="52849at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="Signal recognition particle SRP54 helical bundle" evidence="14">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="95"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="13">
        <location>
            <begin position="96"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="103"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="185"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="245"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEE94306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="449" mass="50212" checksum="DF63569EB6B84750" modified="2011-06-28" version="1">MLDNLRDAVRKFLTGSSSYDKAVEDFIKELQKSLISADVNVKLVFSLTNKIKERLKNEKPPTYIERREWFIKIVYDELSNLFGGDKEPKVIPDKIPYVIMLVGVQGTGKTTTAGKLAYFYKKKGFKVGLVGADVYRPAALEQLQQLGEQIGVPVYGEPGEKDAVGIAKRGVEKFLNEKMEIIIVDTAGRHGYGEEAALLEEMKNIYEAIKPDEVTLVIDASIGQKAYDLASKFNQASKIGTIIITKMDGTAKGGGALSAVAATGATIKFIGTGEKIDELEVFNPRRFVARILGMGDIETILEKIKEVENYDKMQKKMEEVISGKGKLTLRDVYNQLIALRKMGPLSKLFQLLPGIGMLGQIPEDQLKVGEEKMRKWLAIMNSMTYEELDNPSIIDKSRMRRIALGSGTEIEDVKELIEHYNLMQRTIKMLKRRKKDVEKLLGQFGGEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4T6</accession>
    <name>F4B4T6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775018.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4T6"/>
    <dbReference type="STRING" id="933801.Ahos_0209"/>
    <dbReference type="GeneID" id="10599649"/>
    <dbReference type="KEGG" id="aho:Ahos_0209"/>
    <dbReference type="eggNOG" id="arCOG00820">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_3_2_2"/>
    <dbReference type="OrthoDB" id="30861at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF7">
        <property type="entry name" value="TRANSCRIPTION REGULATOR, SPOVT_ABRB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="5"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="80" mass="9179" checksum="6B1AD6F8902A2E81" modified="2011-06-28" version="1">MKTKVRVGKKLTIHIPKAVAEELNIKDGDILSLRVKDNKIILEQDNAILLSLKGKKFAKISLDEIEKISEEEQNKYENFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4W3</accession>
    <name>F4B4W3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Integral transmembrane protein NrfD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NrfD family.</text>
    </comment>
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        <property type="protein sequence ID" value="AEE93128.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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            <begin position="26"/>
            <end position="48"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="142"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="172"/>
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        <location>
            <begin position="205"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="247"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="282"/>
            <end position="303"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="364" mass="40407" checksum="B0A29574646B227A" modified="2011-06-28" version="1">MGLTPPPPPFQYPVSWGPDMPPDHQAIWGAKVALAYFFIGLGSMLAAITAANDVRVSVPATGGVTESIRDIARFKFSVGVASLIALILGLGFLVLDAGKPSTAWQIITYGLQYGRWESWMFLGTIFLIVLFILDIAYSAIWLGNMRPNFLSMLFNKISSWYVVKGKMYSMRYFLIGIMIIFGILGASYGGVLFSQTNIGLWRNPTIILLFPVSGLSSAAATGLLLTLIIKDPSIRDTNLLLWSKIDLYAEASEATLTALFLYIGYISTYASESVYQVLFGRYSIYFWVLVVTLGIAIPMLLELALHKVNPRYHKYIIPIVTVLVLIGAASLRYYVVIASAYFYPPLSPFSPTQMQFPWGTYWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
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    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1432"/>
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    </dbReference>
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            <dbReference type="EMBL" id="AEE94315.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9655" checksum="62B7B8BA9FCA54A9" modified="2011-06-28" version="1">MVYAVVTLIENCGKIKKVPPSLITNYEKFLNFFLPKNLQSLTVILPYEMMDESEKIREAVMKARPSCVVKILVDKDSKEIVFCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B517</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B517"/>
    <dbReference type="KEGG" id="aho:Ahos_1436"/>
    <dbReference type="eggNOG" id="arCOG07720">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_592650_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="416" mass="46769" checksum="10A67183EC538708" modified="2011-06-28" version="1">MLNVNTSIDIIKDAVTCILTKMKGDMRFYPALAVSSIVSKAVKNIFQVIRHGVVIQTPKGNYYYIGGASEYWESSIYAFQASVKFRNNTKHLADKMKGKNVVVIKVRKINSELPQFNPVEGYLPLEPTGAIMTNYLPDLTIQPFSDTEVPGNLIYESNEHYTADVLSKILRVISTRPPFPYMVIATISKQATFKVPPAVQAYVPFPASVMDELCKFFLLGRFKEYCSDLVSNTSYNEAIVGAPLFTTLDCYKPVSLIGLVYDGEMLNNSFARLSVIEPPPHTDGEMLDYANKLGVGEILELSIRGEEYAKKGAKAISSAYGLSPVVANYITNYITWSENEDEMVREAEPYVELVRSTIKQVRKEMDKMRDNRPTEVIEECLARQDIEENCLEVEGCVDLYSATLECLHPELFKDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B530</accession>
    <name>F4B530_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1449</name>
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    <organism evidence="2 3">
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="aho:Ahos_1449"/>
    <dbReference type="eggNOG" id="arCOG06946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="43831at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="185" mass="20422" checksum="5041BD3563116725" modified="2011-06-28" version="1">MCIGLLLDIIFFIIDIIIPIWNSYNSGKISAYRKGLGKLLYTLGGFLPMSYVLSLIIAIVLGILGYISVSTAVFILSFSGLVFGLEIIIWGVIATYLSAVSTVRGRDWKAGLITAYNAFATIFDAWAYISSFFSNLRDARKAIDSSDFSVIDVLIIFVAALGVGFIITYAAYKEGLKSARTRYWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>F4B532</accession>
    <name>F4B532_ACIHW</name>
    <protein>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776249.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B532"/>
    <dbReference type="STRING" id="933801.Ahos_1451"/>
    <dbReference type="GeneID" id="10600947"/>
    <dbReference type="KEGG" id="aho:Ahos_1451"/>
    <dbReference type="eggNOG" id="arCOG01928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136101_0_0_2"/>
    <dbReference type="OrthoDB" id="43181at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="244" mass="28401" checksum="B43ACF3B34FB5579" modified="2011-06-28" version="1">MVIKLTEEIEMNDDESSKLWFYDKKNIIDCIPNLVGFSENKIKLRVKNFFFLNKTLEYEITSFVGGGYIEHKFKNEDSFATLLITFNKNRKIISLSLSYSGKFERQMSKILEEMMKNLKRKYLEQKTVNKKGETISSKLSSLSFVAKLVSTSKLIYSDKLDSGKIDITPFIEDILHKFSQYPIIYISGFNEENTFRLLFVNGELKGVYIKISDKEFYSEEELLKLKGLFHVSVYANLLKSSMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B540</accession>
    <name>F4B540_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Ahos_1459</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776257.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B540"/>
    <dbReference type="STRING" id="933801.Ahos_1459"/>
    <dbReference type="GeneID" id="10600955"/>
    <dbReference type="KEGG" id="aho:Ahos_1459"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_7_0_2"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000006">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="285"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="443"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE94342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="589" mass="65368" checksum="FD23C46832880CBB" modified="2011-06-28" version="1">MCGIIGVISKNDEKKLATITVQCLERLEYRGYDSVGVASMEGNELEVRKAKGRIDEVVKRLNILSMTGRVFLGHTRWATHGEPNDINAHPHTDCTNSIAVVHNGTIKNFRELREDLESLGHKFKSETDTEVIPHLVEEFKKRGMDSFSAFKSAINSIQGSYAVLAIIKGENKIYFAKKDNPLIIGLGDKMNFISSDIPSFLPYTNKVLILIDGDVGYITPDEIYIENEGKKVNVLDRIKIITWDASAASKEGYAHYMLKEIHESPRAVDDTISGLISDIEDVKNAIKILEESKRIIVIAAGTSYHAGLYFSLLLQREGFNVIPVIASEYYNIKTNQEDSILAISQSGETYDVMMALKEFKADGSKIISLTNVIDSAIARQSDVKLYTRAGPEIGVAATKTFTSQIAALQFLYSLIKGDDYTYLNKAKDVIRRSLDFEGEAKLIGEELSAKSNAYYLGRGLSLPFAMEGALKIKEITYLHAEAYAAGESKHGPIALVEKDFPVVFINLGELVGELQNNVQEMKARKAKTYAISVNERLNTDKEILLTVEDERLSPFAVTPIIQLIAYYSAVKRGNDPDKPRNLAKTVTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B593</accession>
    <name>F4B593_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglutaminase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775103.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B593"/>
    <dbReference type="SMR" id="F4B593"/>
    <dbReference type="STRING" id="933801.Ahos_0296"/>
    <dbReference type="GeneID" id="10599736"/>
    <dbReference type="KEGG" id="aho:Ahos_0296"/>
    <dbReference type="eggNOG" id="arCOG02165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140614_0_0_2"/>
    <dbReference type="OrthoDB" id="18481at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013589">
        <property type="entry name" value="Bac_transglu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33490">
        <property type="entry name" value="BLR5614 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33490:SF6">
        <property type="entry name" value="SLL1049 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08379">
        <property type="entry name" value="Bact_transglu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="1">
        <location>
            <begin position="161"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="266" mass="30618" checksum="FF5D91DA26BD774B" modified="2011-06-28" version="1">MDSLKFHVLYEAKYEYEDVVTSNDNTLKIVPYDGDNQELLEENVDTEPKGYKTRYKDMFGNIVYRIKVIEPHYSLVIRSESSVNVKLKEMEDCMLPCQIEDETFLNSSRLIDVDYFKPIAKELLKSSKSLDDLVKNTVEFVRNRLKYKEGITNVDTSAKESFEIGMGVCQDFAQVTIGILRASGIPARYVMGLVNDNPISTHAWVEFKSPSGWIPVDPTRRKFYNIAYVKFAIGRDYYDTPPILGTFVSKGKGWLKKLTVMVRRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B599</accession>
    <name>F4B599_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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            <strain evidence="4 5">W1</strain>
        </source>
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            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <sequence length="245" mass="26692" checksum="394E4514E2ADCF6C" modified="2011-06-28" version="1">MKVGEVMSKIVVTVRSEDSVKEVINALSSTPSGRLVVMSGDEAVGIISSRDVVKAYSKMEDNVFEARADEIATKGVITVDENEEVGNAVRIMASKKIGSLVITSGKVLRGIFTERDVIRLLSKMTFSGLVESIMSTDVITIPSDVDLLFASKLMEYEGVRRLPVVRGKEVEGIITAADIVKALAKGLHMVNEIETRNPIGVRSDDPIMKAVRIMNEKRIGSLLVDGIKGIVTERDVLYASLNEIR</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Shah S.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">W1</strain>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    <dbReference type="GeneID" id="10599744"/>
    <dbReference type="KEGG" id="aho:Ahos_0303"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93194.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="140" mass="15598" checksum="F293DE21233925E9" modified="2011-06-28" version="1">MTSKVKFLISKTPVTAEKGTKLEDVVKIMASMNIGSVIITDKEKPVGIITERDIIRALAKGIPLTEKIENVGTMDLITVFEDDSIYTAAEKMNKYNIRHLVVIDKEGNFKGVISIRDLIRESYVLKALANVSQEEWLGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5E2</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <source>
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        <source>
            <dbReference type="EMBL" id="AEE93236.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="43022" checksum="48FDF8817356F2F4" modified="2011-06-28" version="1">MRKNFLIASWLFGLGNSFSSPIIGLYIYINSSINYMLKFLLISSIFILLGYILVGYLTTFWKEALTYYKLGILLFISFYILMFILNVKSYRFISFLGILYGLAQGFYWSGWDIIFYNIPHKLKFFNKSAYLGFFTNLVSPAVYGSILTIFHNNGYGILFLLTALTLLLSAILAESVKVSSFKFNIRRSISVFNENKTYRYTMTALAIVSGVNYILTNVNTIIIYKIATDYLNFAIISYSMSVITLISIYVIRDKLSGILKPYNVVLSSSLLLGISSVSIIVGFPLFYLIVFSLTSPLIYPIIDVYNWNNMDRRFLTEYLVNRQIFLNSGRILSSLSEVILSQISGYEILPLIPLIIIASIIFVRYNSKEKIQQDVL</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="F4B5F2"/>
    <dbReference type="STRING" id="933801.Ahos_0355"/>
    <dbReference type="GeneID" id="10599796"/>
    <dbReference type="KEGG" id="aho:Ahos_0355"/>
    <dbReference type="eggNOG" id="arCOG00716">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160447_0_0_2"/>
    <dbReference type="OrthoDB" id="21373at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09854">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="130" mass="14863" checksum="C7ED5805ADE552BE" modified="2011-06-28" version="1">MTNKGKYFDVNVFVYYLTGDKTNGERAKNWLSMEDDKYTSVVTPFLLTVVLGKILGRSLKDYDFIKTIGEALESLGIEYLELPIWSKIVDNVKRYDIDLEDSIHVSTALENGLEIISNDSELKKKVKAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5G1</accession>
    <name>F4B5G1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1502</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776300.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5G1"/>
    <dbReference type="STRING" id="933801.Ahos_1502"/>
    <dbReference type="GeneID" id="10601002"/>
    <dbReference type="KEGG" id="aho:Ahos_1502"/>
    <dbReference type="eggNOG" id="arCOG01586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090242_0_0_2"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF8">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="261" mass="30199" checksum="CA994AD60EEBBD14" modified="2011-06-28" version="1">MDELDKKILLYLFRDGRRSIRSLASLLNLTPPTVLYRIKNLEKEEILEGFQLLVNPNFYGKYYSFIAFKNLMDMDDKWVFLKFKCVEWLNVYGIVGGSNAEIEDRISFMRKTLGEPVMQYTPLQQPLPPKELDIKIVKALQEDPRASTAEISKKLGIDSKIVIKRLKIMKNRGYISVIPKINIPKSGMSVFSMFSKRVPEIRDILSPCRVLEIVDKNNVGIEVCLTENIENVRNYVRAVREKDKEADVMIIYDINTKGIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5H4</accession>
    <name>F4B5H4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Isochorismatase related protein (EntB-like1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776313.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5H4"/>
    <dbReference type="STRING" id="933801.Ahos_1515"/>
    <dbReference type="GeneID" id="10601015"/>
    <dbReference type="KEGG" id="aho:Ahos_1515"/>
    <dbReference type="eggNOG" id="arCOG01943">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_8_3_2"/>
    <dbReference type="OrthoDB" id="9194at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00431">
        <property type="entry name" value="cysteine_hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050272">
        <property type="entry name" value="Isochorismatase-like_hydrls"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540:SF6">
        <property type="entry name" value="ISOCHORISMATASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43540">
        <property type="entry name" value="PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="2">
        <location>
            <begin position="18"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="186" mass="21167" checksum="3FBED2052B82047A" modified="2011-06-28" version="1">MAWYNKDEIKKFIRKDNSILVVWDVQEALVNSIFNKEEFLQKLKEIISAARQYNVPIVYTKITPLPERFQPPYMRRSFNPGDIVKDVYPQGSDVVLNKNTTSIFVGTNFELMLRNAGIQTIVFTGIATDIGVETSARHAQALGYLPVIAKEAVSSSDKEAHERSLANMSKLMLVLDNKEIIERWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K3</accession>
    <name>F4B5K3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776342.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K3"/>
    <dbReference type="STRING" id="933801.Ahos_1544"/>
    <dbReference type="GeneID" id="10601044"/>
    <dbReference type="KEGG" id="aho:Ahos_1544"/>
    <dbReference type="eggNOG" id="arCOG01561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707133_0_0_2"/>
    <dbReference type="OrthoDB" id="372855at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="4"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="390" mass="45226" checksum="94D2B9E082B42EB6" modified="2011-06-28" version="1">MYLISLLGNGDTGKTTSFAKFLLKNNYFLDHQEEAVKNAKFDAEINGKPDDYYQFLLYVSPQEKIEERTIYTAQKIMAYHQGKFLPFRTISYLKFTGEKYERVIVDSPYKDGFAKGYVTFSLQDIGGQFDLFDKQINGLRISDSVIYFADDKTNEKDVENHAKMISAFNLPTFVVVNKEDILKEKAVEKMNMILTIFQEHKINVVDKIVTSALYKEDEIVKFFENAIKWTIDKDIRIYGKNIFGIIRNVSRSIATMRAYTSLSMPKNSQQYLYTQRKKRFFPNKVKSVEFALKETRMIHKGELIGVAFSENPLPSTVFKEEEHIRNPITNIHLERPLQEGEILFSSLVHSIKGEKYGNLILNEGIGVNELFVINTINQTIKEIYDLTDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5K5</accession>
    <name>F4B5K5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase beta domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776344.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5K5"/>
    <dbReference type="GeneID" id="10601046"/>
    <dbReference type="KEGG" id="aho:Ahos_1546"/>
    <dbReference type="eggNOG" id="arCOG01197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_9_4_2"/>
    <dbReference type="OrthoDB" id="9287at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NT_sf"/>
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        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
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        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POLYMERASE BETA NUCLEOTIDYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="104" mass="12584" checksum="10E63DF2EDF33EAB" modified="2011-06-28" version="1">MLRRAELFIEDVKKEINVKEVYVVGSRARGDYLDTSDIDLVIISDDFKGMRYIDRMEKLSKYRRAKIYYFTFTNEEWENPQSLYIEEMKKEAKKLDELLRNLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5L9</accession>
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            <fullName evidence="2">Amino acid permease-associated region</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Ahos_1560</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5L9"/>
    <dbReference type="STRING" id="933801.Ahos_1560"/>
    <dbReference type="GeneID" id="10601061"/>
    <dbReference type="KEGG" id="aho:Ahos_1560"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2801990_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052962">
        <property type="entry name" value="AA_Transporter_AGT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47547:SF1">
        <property type="entry name" value="ASPARTATE-PROTON SYMPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="67" mass="7264" checksum="3AF8F23E604B00C4" modified="2011-06-28" version="1">MIKKPTRLGTESDQKISKSLSRMELFYIGLGGIIGSGWLFATLYVAEDAGGASILSWIIGGEWIRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q4</accession>
    <name>F4B5Q4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas1</name>
        <name evidence="10" type="ordered locus">Ahos_0378</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Q4"/>
    <dbReference type="STRING" id="933801.Ahos_0378"/>
    <dbReference type="KEGG" id="aho:Ahos_0378"/>
    <dbReference type="eggNOG" id="arCOG01452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09636">
        <property type="entry name" value="Cas1_I-II-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="entry name" value="CRISPR-associated endonuclease Cas1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050646">
        <property type="entry name" value="Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34353:SF2">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDONUCLEASE CAS1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="310" mass="35965" checksum="4A4CE4BC3B89E9DC" modified="2011-06-28" version="1">MVYNSVIPNFPMDKKIAFVKDYGAYVRVNNGLFECVVNKEVKWKLSPVELHSIVFLVNSSLSSEVVRLANENGVDLVFFYNHEPYAKLIPAKYGGSFKLWTMQLKSRKRKVEFARSFIHGKIHNQLVTLRYYEKKYGYDLNTKRLDELSTEVQFVESVEEVMTKEAEAARIYWRGVKSLLPKSLGFKGRRKRQSENIDVFNVALNIGYGMLRKIVWSAIVSVGLNPYIGFLHKFREGRPSLVFDLMEEFRSPFVDKPLIGMARERPDDVSEAREVYSLLSRINEEEIYTQARRLAKAIIEGVEYTPFMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Q9</accession>
    <name>F4B5Q9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
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    </dbReference>
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        <location>
            <begin position="15"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="390" mass="44978" checksum="F7B8A44BFA902F9A" modified="2011-06-28" version="1">MLFDVRPKEDRKDLYDRDKEVDEIKESIDRKVWIAVYGMRRVGKTSVVNVAVNESRYIVIKLNLMRIYDPKKKKYPKTSFVSLFLESINEAVKKYTLGGRLIRFVSNVLGIDENSFIDFNLVKIRPRLKKFRGEDVSSTIRELDLLAGDNKRTLVLIFDEAQELMKVNGINFSSVFHDIYDYCKNTTVIFTGSSVGILENMLKDLEYEKPFFGRFIRRIKIERFNEQLSRDFLLKGFAEEGVTVQEDVINDALRRFDGIPGWLTFFGSEYSFRIRHGEKQVNVDDIEKMTVEEVRKEVKDFILGTQSPERYSAAILALDRLGGKGSAADVTKVVSSILGNVPEPRIYEILNRLVNMGFIEKENEEYSLPKDEPDRKGLVLAAKDVMSLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
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            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">W1</strain>
        </source>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10599827"/>
    <dbReference type="KEGG" id="aho:Ahos_0387"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="L-sulfolactate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="EMBL" id="AEE93276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="98" mass="11077" checksum="B3DE42D0C2BC863E" modified="2011-06-28" version="1">MKVNVEEAKELVYKIFSKVTYAEYAKYLSEELIEAEIEGHSDHGLQLIPYYIKLANGEEVDIGGQKIPPINPKGKVEISGENLGRNKEIFTYMSSTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5Z4</accession>
    <name>F4B5Z4_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">PaREP1 domain protein</fullName>
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        <name evidence="1" type="ordered locus">Ahos_1614</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
    <dbReference type="RefSeq" id="WP_013776407.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5Z4"/>
    <dbReference type="GeneID" id="10601114"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PaREP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237:SF4">
        <property type="entry name" value="PAREP1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34237">
        <property type="entry name" value="PAREP8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05942">
        <property type="entry name" value="PaREP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="153" mass="17714" checksum="379064288E61F8B2" modified="2011-06-28" version="1">MEELIKKAEDIGINVEDVLISKNDPKEEIKLRLDLAKKYMKECEEYLKKGDAIQASEKAYKVAEELIKALSEKFNLEEYQKTLKEGRWYTYLLVSASSKLSQKLGDWVLSGWDAGYSLHVWGFHEAKLSVSDIIPRVEKVRKMLEESEKILTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B617</accession>
    <name>F4B617_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS transporter</fullName>
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    <gene>
        <name evidence="2" type="ordered locus">Ahos_1637</name>
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    <organism evidence="2 3">
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776430.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B617"/>
    <dbReference type="STRING" id="933801.Ahos_1637"/>
    <dbReference type="GeneID" id="10601137"/>
    <dbReference type="KEGG" id="aho:Ahos_1637"/>
    <dbReference type="eggNOG" id="arCOG08557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_770788_0_0_2"/>
    <dbReference type="OrthoDB" id="44242at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41648" checksum="F89A4099E7236D3E" modified="2011-06-28" version="1">MPTYKNKVILFRSIYSVFLGFMSFGYIAIMSNSGIPYTQISLLVTLNFLFQTVFYLVFSVVRIRKGLIISSFLNLIVILILLSERNFLTFFIISLILGLADALFSSIVIIQKDFKKKDYSVLMSLTFVANVLGLGLYYFSFYIRLEYLLILLAVISSVNLFVSLSLEYEEEDLKFEDFLQFIKIKGKFLITLDGLATLRRVFANSILPIVFISVFSYISREYLSLYFAVFQLPLVFFLYYGQKVSNKSYLLLTFSEFFIYILLIRFYYLSPIIFLGFILLIKSTEYLRAPIAEAALLQKIEYSTKVMSFFNFLDSILGVISGSIFTALIFIKAYSLVLLLAGVSSLSADLFLYRIIKEYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>F4B647</accession>
    <name>F4B647_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5 7">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="4">P5C reductase</shortName>
            <shortName evidence="4">P5CR</shortName>
            <ecNumber evidence="4 5 7">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">proC</name>
        <name evidence="10" type="ordered locus">Ahos_0447</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 5 7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B647"/>
    <dbReference type="STRING" id="933801.Ahos_0447"/>
    <dbReference type="KEGG" id="aho:Ahos_0447"/>
    <dbReference type="eggNOG" id="arCOG00455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_1_2_2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="9">
        <location>
            <begin position="155"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="268" mass="29147" checksum="568CBC48672097C4" modified="2011-06-28" version="1">MSVFAYPFMRIAIIGAGKIGFTILSALNKMSNVELIATGRSNSTLEKIKSIGVEATTDNNYAVSSSDYIILSVKPQHFPSVLKSVKSWEGKKVVSVMAGIRISTLSSLLKGSKVYRAMPNINAIIGKSTTALAEERDETVEKIFLQIGKVYWVPEELMDAWTALIGSGPAFIAEIIDALSLGAVECGMPRDLSYNAILDMIEGTVELLRKCKHPALLRDQVTTPAGTTIRGLMTMESEGIKSALIKTIEAAYKRSAEIGEQIDRKLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B658</accession>
    <name>F4B658_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B658"/>
    <dbReference type="STRING" id="933801.Ahos_0458"/>
    <dbReference type="KEGG" id="aho:Ahos_0458"/>
    <dbReference type="eggNOG" id="arCOG08402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_207589_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="68" mass="7735" checksum="C4328EED470DDA74" modified="2011-06-28" version="1">MIPYLMTAHEGRSDKSVDGRVVVFPSTNPKDLRVNMYDPLRGVPVAELKVRISYATGKHCNYTFHFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>F4B668</accession>
    <name>F4B668_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B668"/>
    <dbReference type="KEGG" id="aho:Ahos_0468"/>
    <dbReference type="eggNOG" id="arCOG01665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3038829_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="54" mass="6254" checksum="9E4D3CB3E976F374" modified="2011-06-28" version="1">MVSIEVTRNIRILYSMDLKNCIVFIWKIGSHKKERLAFFSTSSKNLSISFSNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>F4B688</accession>
    <name>F4B688_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CoA-disulfide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_0488</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775292.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B688"/>
    <dbReference type="STRING" id="933801.Ahos_0488"/>
    <dbReference type="GeneID" id="10599928"/>
    <dbReference type="KEGG" id="aho:Ahos_0488"/>
    <dbReference type="eggNOG" id="arCOG01069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF1">
        <property type="entry name" value="NAD(P)H SULFUR OXIDOREDUCTASE (COA-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="3"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="7">
        <location>
            <begin position="319"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE93376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="436" mass="48046" checksum="C56298152346D817" modified="2011-06-28" version="1">MDKVVVLGGGAAGMSASSRVRRLNPEAEITVFEETKMVSHAPCGVPYFVEGLFNDENLFMTYTPSFFIEKRKINVKINEKVEEVDLSSRVVKVNENGTRKNVEFDYLVITLGSKPKKVKEEGDRIFYAHHPANAVELRKALWSLNKIAIIGGGILGVEMAEALSNIGKKVILIHRGGYVLNKMLDEDMGKVITSSMDVELRLNESLEEIKEGGRVVITDRGKYEVDGTLIAIGVEPNVDLVKDQVKIGETGAIWVDSHMRTSVRNVYAAGDVAESIHMVTKKPVWMPFAPVANKMGFVAGSNIAGKEMEFPGVVGTMITKYKDYFIAKTGISEAEAKRYGIKVYSAMIKSKTRARYYPGGKDIYVKLIAEEGSNKVVGGQIIGGEEVLGRIDMLAAIIMKGFTVEETFFVEMGYLPAIATVWDPVIIATRQLMKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B689</accession>
    <name>F4B689_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775293.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B689"/>
    <dbReference type="STRING" id="933801.Ahos_0489"/>
    <dbReference type="GeneID" id="10599929"/>
    <dbReference type="KEGG" id="aho:Ahos_0489"/>
    <dbReference type="eggNOG" id="arCOG05898">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149090_0_0_2"/>
    <dbReference type="OrthoDB" id="36993at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="145" mass="16440" checksum="0A3C3F8F0DF0F200" modified="2011-06-28" version="1">MIAKDKISSLREELNNLSETNLSIAEQGILQFIKEQVDKEEELVSNFDKSTSENNYDKAISDFFQLIQRTNLMYAYIMQPSVLSMLSNEKFSKIVQDLLDCISQIISDVVLTFKHNMKQFGLDNVNVNITSNPPSISISLAIKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6B2</accession>
    <name>F4B6B2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0512</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. ABHD14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775316.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6B2"/>
    <dbReference type="STRING" id="933801.Ahos_0512"/>
    <dbReference type="GeneID" id="10599953"/>
    <dbReference type="KEGG" id="aho:Ahos_0512"/>
    <dbReference type="eggNOG" id="arCOG01648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_28_1_2"/>
    <dbReference type="OrthoDB" id="7531at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197:SF3">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197">
        <property type="entry name" value="PROTEIN ABHD14B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="25"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="195" mass="21297" checksum="62EA7ABE88938A9F" modified="2011-06-28" version="1">MQLEDKFIEVKGAKIHYLEEGSGRPVVLHHGARFNAYTWKEVGTISAIAEVGYRAISIDFPGFGKSEEGNFSSLSEFIGDFIDTMKLEKPILLGASMGGEAVLGYAVDHANKLGGLILVGAVGVSSYESKLKNLDGLPILLIWGRHDTTSPKRNAELIMKYVKTAKFVNVGNQHACYLDDSEGFNSQIKDFLKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6C8</accession>
    <name>F4B6C8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetrathionate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776463.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6C8"/>
    <dbReference type="STRING" id="933801.Ahos_1670"/>
    <dbReference type="GeneID" id="10601170"/>
    <dbReference type="KEGG" id="aho:Ahos_1670"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030956_0_0_2"/>
    <dbReference type="OrthoDB" id="8638at2157"/>
    <dbReference type="BRENDA" id="3.12.1.B1">
        <property type="organism ID" value="13539"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512">
        <property type="entry name" value="CELL SURFACE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34512:SF30">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
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    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="1">
        <location>
            <begin position="148"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="516" mass="55612" checksum="35E2013D8F04A11D" modified="2011-06-28" version="1">MTFLNFTSIVAQDKGDQQPKGPIVYTYDEYNGTYFPHILTVVYYPCNATCSNLGFPSVWPVSNENQCHNAVVNTTCQALIEGVDWQLPLLNYVGGVAIPLSTPPCKLPWAQQLGVKGALVYYTQMVGEPLGVTLACGLLYATEDSMPGSITAINPVTGKIVWMANGLAGPAMNNPVVWHGIVYVSVGGVCFTFSQFVHFEEHQYCKIHRGRCGAVYAFNATDGQLLWMRFTYGEAMPAPAIYDGILAYVTGGGCFVGVNATTGQTLWVDHFPGFMANMASVNYYVLPNGTPLFIAGFTYAAAPYGYIIAVNGLNGHEAWNATMPAPYVGANTGLGDVAPAVDQQLGIVVDNDIANFSNGYVDMVTFALNATNGKPLWAVNTGRGPIPPAYKGGMPLIVGNVVYDGNPSLGTVNAICIKTGKILWSTKLPCLQTPPKFPGGPRGSPTYYHGLLWVSAGQYVYVINPKNGDILTFYWIGGRLGIMNPVIAGNTMFLSNSYGWIVAIPLSQIYPAYIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G2</accession>
    <name>F4B6G2_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">UPF0033 domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1705</name>
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    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776497.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6G2"/>
    <dbReference type="STRING" id="933801.Ahos_1705"/>
    <dbReference type="GeneID" id="10601205"/>
    <dbReference type="KEGG" id="aho:Ahos_1705"/>
    <dbReference type="eggNOG" id="arCOG02072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165242_0_0_2"/>
    <dbReference type="OrthoDB" id="33202at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF18">
        <property type="entry name" value="SULFUR CARRIER PROTEIN MJ0990-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="86" mass="9806" checksum="F0752C2D836E1C99" modified="2011-06-28" version="1">MEELNLVSLECPEPFLKVSAKIMEMKGGEQLKVLYKDPKCDEMLLQLMDLANCKVLQHEEKDGVFTLIIEKKGEKKKVDLSDFTGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G7</accession>
    <name>F4B6G7_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">OrfB transposable element</fullName>
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    <gene>
        <name evidence="1" type="ordered locus">Ahos_1710</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6G7"/>
    <dbReference type="STRING" id="933801.Ahos_1710"/>
    <dbReference type="KEGG" id="aho:Ahos_1710"/>
    <dbReference type="HOGENOM" id="CLU_161114_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="127" mass="14489" checksum="C3BB69914BFFB0C9" modified="2011-06-28" version="1">MQITKMGKENLVNDSPLIDLKISFSYCHSIARREKNPIRATVAMNIGLSDSILALVNNYALRFALFWMKESVKNPEEKDTLSKVHAGLYEKLKVEYNLPSKVAEDCYRDALSIYKFNPKRGRFPACG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6G9</accession>
    <name>F4B6G9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776504.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6G9"/>
    <dbReference type="STRING" id="933801.Ahos_1712"/>
    <dbReference type="GeneID" id="10601212"/>
    <dbReference type="KEGG" id="aho:Ahos_1712"/>
    <dbReference type="eggNOG" id="arCOG00815">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_9_3_2"/>
    <dbReference type="OrthoDB" id="30861at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF6">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="77" mass="9023" checksum="CA2567C4303FFAC8" modified="2011-06-28" version="1">MKVKVTRNFQVTIPSEIREKLGIKEGDYVEVTLDESNGVIIIKPYIRKWTTIRLNRKVDQEDIDKAVEEALNDNSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6K6</accession>
    <name>F4B6K6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775332.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6K6"/>
    <dbReference type="STRING" id="933801.Ahos_0528"/>
    <dbReference type="GeneID" id="10599969"/>
    <dbReference type="KEGG" id="aho:Ahos_0528"/>
    <dbReference type="eggNOG" id="arCOG00417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1471995_0_0_2"/>
    <dbReference type="OrthoDB" id="17644at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="183" mass="21026" checksum="5613529EB97F7665" modified="2011-06-28" version="1">MEYKHCKLSEFIFKNGNLVSLYGVAGSGKTAVALQVLEDITPSIYISTQGKSYEARVESMALINKNTFFVEANSADELVENIIRGIQLQPELLIVDSINTFYRLSRRAVDLIHPLIFLKEFSKYKKAIITWQMSMNNKVSGEKFMRYFSDDVIRVTKNYLIGNLRECKFKITERGVIGCLRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6L0</accession>
    <name>F4B6L0_ACIHW</name>
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        <recommendedName>
            <fullName evidence="2">UPF0148 protein Ahos_0532</fullName>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0148 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6L0"/>
    <dbReference type="STRING" id="933801.Ahos_0532"/>
    <dbReference type="GeneID" id="10599973"/>
    <dbReference type="KEGG" id="aho:Ahos_0532"/>
    <dbReference type="eggNOG" id="arCOG00578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142653_1_0_2"/>
    <dbReference type="OrthoDB" id="26305at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00343">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022954">
        <property type="entry name" value="UPF0148"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001644">
        <property type="entry name" value="PRK00420.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001647">
        <property type="entry name" value="PRK00420.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="115" mass="12983" checksum="C3262F68F6EE78B9" modified="2011-06-28" version="1">MTDDSIKKAAELLRQGATMLSEACPVCGSPLFRLKNGDVVCPVHGKVYIVKSDEEEKQIKKDIMLTSVEDFLVEGLYSTAKKIKEDPYDSETLVQIIRYLDAIERIRKLVPQHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M6</accession>
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        <name evidence="1" type="ordered locus">Ahos_0548</name>
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    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M6"/>
    <dbReference type="STRING" id="933801.Ahos_0548"/>
    <dbReference type="KEGG" id="aho:Ahos_0548"/>
    <dbReference type="eggNOG" id="arCOG08338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2550236_0_0_2"/>
    <dbReference type="OrthoDB" id="33048at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="84" mass="9529" checksum="A654643B8E65DACB" modified="2011-06-28" version="1">MEVEEDNKKYYAVVKVDNLDLPDNVGVSRLHSHISSAVDEALENLKEYLKEQGISGKFSTNVEVFVKEETMSRLVETIKAKIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6V3</accession>
    <name>F4B6V3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776561.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6V3"/>
    <dbReference type="STRING" id="933801.Ahos_1770"/>
    <dbReference type="GeneID" id="10601270"/>
    <dbReference type="KEGG" id="aho:Ahos_1770"/>
    <dbReference type="eggNOG" id="arCOG01423">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_955106_0_0_2"/>
    <dbReference type="OrthoDB" id="43195at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314">
        <property type="entry name" value="CRENARCHAEAL PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34314:SF6">
        <property type="entry name" value="DUF3782 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47162">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="291" mass="33566" checksum="8E9C77DB238F2923" modified="2011-06-28" version="1">MSEEIRKWFNDFVNSIVEKIKTGKELNALETQIISNYASNLVLYDYVDKRLGELERNLRDEIRKTREELLANDEKVKQELLANDERIKQELLANDERIKQELLQKIEEVKKELLANDEKVKQELLANDERIKQELLQKIEEVKKELLANDERIKQGLKAEIQSVKTDLENKIKEVDRKVEATRSDLGLLAEEVYVKNFVDYLESNGEKVVSVYRHYETSLGEIDAVVETSGKVYVVEVKMRAEVSDVDDLLNKTKVLKEELGGKEVYPVLTGSKINKTVRGYAKGLGVLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6X6</accession>
    <name>F4B6X6_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">PIN domain protein</fullName>
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    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776584.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6X6"/>
    <dbReference type="GeneID" id="32173782"/>
    <dbReference type="KEGG" id="aho:Ahos_1793"/>
    <dbReference type="eggNOG" id="arCOG00715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2339986_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="97" mass="11329" checksum="A29B994DC32A4A30" modified="2011-06-28" version="1">MEDKILVYPKVSNLSKLIDNVEVDELPDDLHELKHKVYRFYIFIYAILKPKKAINEKVTKMKEKAEEVLSRIYKGEEKVITTVVHISEVANIIETNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B703</accession>
    <name>F4B703_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775402.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B703"/>
    <dbReference type="STRING" id="933801.Ahos_0598"/>
    <dbReference type="GeneID" id="10600045"/>
    <dbReference type="KEGG" id="aho:Ahos_0598"/>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140139_0_0_2"/>
    <dbReference type="OrthoDB" id="18902at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="161" mass="18286" checksum="D491ACFA75F19673" modified="2011-06-28" version="1">MSQPGQTKTKELKTITKQVITPSVLKEDKKKVQLMYIIKLFGGISERALITLLYEASQKGLDLGYKFNVIGNNVFSPMIKEDLTSLLYLGLIESDPVNKKLKLSTNGEEALEENQAVIDDNFKNQLNQILSEIKSKIVAIDEEYTLKLRNANRGYSRGRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B741</accession>
    <name>F4B741_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B741"/>
    <dbReference type="KEGG" id="aho:Ahos_0637"/>
    <dbReference type="eggNOG" id="arCOG01669">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152462_0_0_2"/>
    <dbReference type="OrthoDB" id="39132at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="111" mass="12219" checksum="1C5920646FEFFC39" modified="2011-06-28" version="1">MTGRENFAKLLANIGMATKMKTADPQLTVELIFLTPAVETLNKRQVMFKPVLEAIKEAKKTGIKIVACEVAMANVGLQKEDIEYGLVDEFASVGGLYVLQKIKEGYEVLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B769</accession>
    <name>F4B769_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="8">CDG synthase</shortName>
            <ecNumber evidence="8">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">queE</name>
        <name evidence="10" type="ordered locus">Ahos_1814</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-carbaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="8">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776604.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B769"/>
    <dbReference type="STRING" id="933801.Ahos_1814"/>
    <dbReference type="GeneID" id="10601314"/>
    <dbReference type="KEGG" id="aho:Ahos_1814"/>
    <dbReference type="eggNOG" id="arCOG02173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066739_2_4_2"/>
    <dbReference type="OrthoDB" id="371936at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836:SF1">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="27"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="205" mass="24041" checksum="6FC3D4A56FC6E7A5" modified="2011-06-28" version="1">MLPISEIFVSLQGEGPFSGRRALFIRFFGCNLRCSWCDTKYSYYGKPKVLEDLDVVDEFIILTGGEPTLYQDILRNFLTKAKSKGSEILVETNGTVLLKDHFVDYVDYFSISPKLSNAGVKYRVDVVKKNVEKLKIKEKFYYIKFPVMWEDDLNEVKKMVEYFGVEKSKVWLQPINNERIDYWWDIAVREGYNLSIQLHKFVSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7D4</accession>
    <name>F4B7D4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776669.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7D4"/>
    <dbReference type="STRING" id="933801.Ahos_1881"/>
    <dbReference type="GeneID" id="10601380"/>
    <dbReference type="KEGG" id="aho:Ahos_1881"/>
    <dbReference type="eggNOG" id="arCOG03856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122664_0_0_2"/>
    <dbReference type="OrthoDB" id="34768at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="256" mass="28500" checksum="644E9E8CA9706516" modified="2011-06-28" version="1">MRKERIANAFVFFTVAVITYFISFNKGLIFTTSAVSSLSFWSGILLVIPTKFFKVILSVKKTKAYWISFGLYLFFHVTLYGFFYGIILGLDVFIPVVSVSYGVGIPPSSILLLPYWESLSPALNIILFGYEDVISPFITFVGIILALLIGANIEKILELKREVSKRKVSSVLIGVPVIGVVSGTSCCLSLPSIIIYFVALDVGVISSVLPILASPIYFSFVWYGLPIGSTVLLFENFREMNKILQRLSKKKECQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7F2</accession>
    <name>F4B7F2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase</fullName>
            <ecNumber evidence="7">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_0670</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="7"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775473.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7F2"/>
    <dbReference type="STRING" id="933801.Ahos_0670"/>
    <dbReference type="GeneID" id="10600121"/>
    <dbReference type="KEGG" id="aho:Ahos_0670"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_0_2"/>
    <dbReference type="OrthoDB" id="323192at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05781">
        <property type="entry name" value="DNA_polB_B3_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05536">
        <property type="entry name" value="POLBc_B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="9">
        <location>
            <begin position="114"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="8">
        <location>
            <begin position="380"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="780" mass="90197" checksum="4F0CD989E9E37D67" modified="2011-06-28" version="1">MIGCFYILDFSYDVEEGKPVIYIWAIDKEGNRVAVLEKNFRPYFYAILDDSADTNKVAEDIKRLSVSQSPITSVKLEERKYYGNPVKVLRIETVIPAYVRTYRDKVAKVRGVKEVVEADIRFYMRYSIDNDLKPFNWFKAEVEEIQDSKLRVKKVYELKKIIQIYEDKPPELRIMAFDIEVYNKYGSPNPRRDPVIIIGVWTNNGGKQFVMKDDDLEIIREFAKFVLDYDPDIILGYNSNGFDWPYLLERVNSRGIKLDIGRKVNSEPSQGTYGHYSVVGRLNVDLLGFASSIAEVKVKSLDNIADYLGVVKKTERVNLEWYQIPEYWSDPSKRDILLKYNMDDVRSTYLLKDVFLPFGEQLTMITGLPLDQLSMASVGYRVEWLLMREAYKFNEIIPNRVEREYESYKGGLVISPAPGIHEGVYVLDFSSMYPSIMIKYNIGPDTLVKGECEDCWVAPEVGHKFRKSPDGFYKRILQKLVEERKAVKSKIPTEKDEYERRRLDERQRALKVMANAFYGYMGWLGARWYSKEGAEAVTAWGREIISSVAKLVEERGFKVIYGDTDSVFIKGSGNVDSLVNEIMNKFGLEIKIDKIYKKIFFTENKKRYAGITEDGKIDIVGFEAVRGDWCDLAKDLQRRVIEKILTSGVDEAVKLVRDVIMKIRRKEVPIQDLVIWKSLDKSLEEYEVDAPHVNAAKKAMKAGYVIFKGGKIGYVIVKGSGKISERAEPYFMVNDINRIDIDYYIDKQIVPSAMRILEQFGIKESTLKNTSFDILNFFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7H0</accession>
    <name>F4B7H0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphate transport regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775491.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7H0"/>
    <dbReference type="STRING" id="933801.Ahos_0688"/>
    <dbReference type="GeneID" id="10600140"/>
    <dbReference type="KEGG" id="aho:Ahos_0688"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1280848_0_0_2"/>
    <dbReference type="OrthoDB" id="33165at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="212" mass="24290" checksum="8891048519ACA2AC" modified="2011-06-28" version="1">MSIAHITLEEKLQQITVKLIDETRALYEFLTVDDNSINPMQIYAKINGIKDSVENEKYLTGEYIIKVREGLDYVDLYIDILNNLEKIAQNIAAATYRLSVLLTRSSKIDNIMHGLIVVMCEKILASLTHLIESIKTLSSSAKKSIESARNILKLEEDVDDLYRNIELKLFEKPHDDLVYVMLMKDIADRLEDSEDLIRDSANSITYIAFSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J3</accession>
    <name>F4B7J3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo7</name>
        <name evidence="4" type="synonym">rpoE</name>
        <name evidence="6" type="ordered locus">Ahos_0711</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775514.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J3"/>
    <dbReference type="STRING" id="933801.Ahos_0711"/>
    <dbReference type="GeneID" id="10600165"/>
    <dbReference type="KEGG" id="aho:Ahos_0711"/>
    <dbReference type="eggNOG" id="arCOG00675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117966_0_0_2"/>
    <dbReference type="OrthoDB" id="7927at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04331">
        <property type="entry name" value="RNAP_E_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04460">
        <property type="entry name" value="S1_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006333">
        <property type="entry name" value="PRK08563.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="175" mass="19156" checksum="27D8459447A3897F" modified="2011-06-28" version="1">MFKIIKAKGVVRIPPEYFGEELDKIALEILKSEYQDKIFKDLGLVLGVLSAKASEEGQIIFGDGATYHEVEFELLAYVPALQEVVEGTVAEVDNYGIYVNLGPMDGLVHISQIADDTFKFDSTRGVLIGEKSKKVLQKGDLVRARIVTVSAVSSSGKLPRIGLTMKQPTLGKLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7J5</accession>
    <name>F4B7J5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="10">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">eif2g</name>
        <name evidence="12" type="ordered locus">Ahos_0713</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="10"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775516.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7J5"/>
    <dbReference type="STRING" id="933801.Ahos_0713"/>
    <dbReference type="GeneID" id="10600167"/>
    <dbReference type="KEGG" id="aho:Ahos_0713"/>
    <dbReference type="eggNOG" id="arCOG01563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_2"/>
    <dbReference type="OrthoDB" id="7798at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000075">
        <property type="entry name" value="Translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="7"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AEE93600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="415" mass="45584" checksum="A52017B0627C8240" modified="2011-06-28" version="1">MSWPKVQPEVNIGVVGHVDHGKTTLVQALTGIWTSKHSEELKRGMTIKLGYAEASFGICKNCKKPDAYVNEESCRQCGSDEEPEFLRRVSFLDAPGHEILMATMLSGTAILDGAILVVAANEPFPQPQTREHFVALGIVGIKNLVIVQNKIDVVTRDQAIAQYKQITEFIKGTWAEGSPIIPVSALHKINIDALIEAIEERIPTPKRDLTKTPIMLVVRSFDVNKPGTTFDKLVGGVVGGSIIQGKFSIGQEIKILPGIRVEDKGKVTYEPIYTKISSLRFGDLEVNEAKPGGLVAVGTYLDPSVTKADSLMGNMVTDAKADIPVLWNIRVEYNLLERVVGSKDLVKVEPIRNKEVLMITVGSATTLGTVTHAKSNEIELELKKPIPVWENNLRLVISRQVGGRWRLVGWGQIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7K0</accession>
    <name>F4B7K0_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methionine synthase</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">metE</name>
        <name evidence="10" type="ordered locus">Ahos_0718</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a methyl group to L-homocysteine resulting in methionine formation. The physiological methyl donor is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the archaeal MetE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7K0"/>
    <dbReference type="STRING" id="933801.Ahos_0718"/>
    <dbReference type="KEGG" id="aho:Ahos_0718"/>
    <dbReference type="eggNOG" id="arCOG01876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="UniPathway" id="UPA00051"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00288">
        <property type="entry name" value="MetE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022921">
        <property type="entry name" value="MetE_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003317">
        <property type="entry name" value="PRK04326.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE93605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="326" mass="37476" checksum="BB23E9A2DF4D39F4" modified="2011-06-28" version="1">MDELPILPTTVIGSYPRPKWLREAIRLHKAGKVSDEELTEAFNDAALAVLHDHYLAGIDVPTDGEVKRDEMVEYFAERLGGFKFYGPVRVWGTAYYRKPAVVGKIEYKSPLLVDEFNYTKSVSYTPNVKITITGPYTIAYWSYNEYYKDMKDLVFDLAKIINKELHNLVDAGARIIQIDEPAIHSNKKDVSWAVEAVNEAVKGINAKLVVHICYGDYRIVAPYLNEFKVDQINFALKIYNYRYLELFKKYGYEKELGLGVIDVHSRRVESAEEVAKDIKNSLEYFPAEKIWINPDCGLKLLPRSIAFQKLVNMVKGTKMVREELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7T7</accession>
    <name>F4B7T7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_1961</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776749.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7T7"/>
    <dbReference type="STRING" id="933801.Ahos_1961"/>
    <dbReference type="GeneID" id="10601460"/>
    <dbReference type="KEGG" id="aho:Ahos_1961"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OrthoDB" id="87732at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051782">
        <property type="entry name" value="ABC_Transporter_VariousFunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42939:SF1">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN ALBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="233" mass="25863" checksum="73F241FF2109E9B6" modified="2011-06-28" version="1">MECISLKNVVKRFGSVIALNNLSFSIPCGGRFALLGPNGAGKSTTMKILAGLLKPDYGEVEIMGMKPGMKEVKKILGYLPEDPMPYRILTVRENLEYIASLRGVPKEKVEEMLDILDLRQYEKIQAGKLSRGNQQKLSLALVLIHNPEIVLLDEPLNYLDIPTQEKVIGILKGMKSTFLISTHIMSIATRLTDHVIMISKGTVIWQGSIEELKALGKEDEPIESVVARMMDNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7W1</accession>
    <name>F4B7W1_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14 17">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="14">NDK</shortName>
            <shortName evidence="14">NDP kinase</shortName>
            <ecNumber evidence="3 14 17">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ndk</name>
        <name evidence="19" type="ordered locus">Ahos_0752</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 17">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15 16">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3 14 17"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775554.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7W1"/>
    <dbReference type="STRING" id="933801.Ahos_0752"/>
    <dbReference type="GeneID" id="10600209"/>
    <dbReference type="GeneID" id="42780381"/>
    <dbReference type="KEGG" id="aho:Ahos_0752"/>
    <dbReference type="eggNOG" id="arCOG04313">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
    <dbReference type="OrthoDB" id="6874at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000003">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 14" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="9 14 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="18">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="14 15">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AEE93638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="145" mass="16309" checksum="26AB374E9313C951" modified="2011-06-28" version="1">MVSQLEFVMIKPDGVKRRLIGEIISRFEKRGLNIIAMKMVRISRETAEKLYEEHKGKSFFEDLISYITSGPVVCMVIEGDEAVSVIRKMIGATDPKEALPGTIRGDFALSKAENVIHASDSPEKAKKEMSLFFEETELQLNTQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y0</accession>
    <name>F4B7Y0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine triad (HIT) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_0771</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y0"/>
    <dbReference type="STRING" id="933801.Ahos_0771"/>
    <dbReference type="KEGG" id="aho:Ahos_0771"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_1_2_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052908">
        <property type="entry name" value="AP-4-A_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997:SF1">
        <property type="entry name" value="AP-4-A PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42997">
        <property type="entry name" value="HIT FAMILY HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="3">
        <location>
            <begin position="33"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE93657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="180" mass="20842" checksum="4EA91E839B69B381" modified="2011-06-28" version="1">MANIFNIMDFLWAPWRSKYVADSSKSKREEKCIFCEFPEQNDDERNLIVYRSKYAYIILNRFPYNPSHVMIVPYRHISSVELLEDNEAQDIFRLIKITLAAIRKVYSPDGFNIGINIGRTAGAGIEAHVHVHIVPRWNGDANFMPVLSNTKVLPEDLETTYVKLKKAIEDIINNEDSLDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Y6</accession>
    <name>F4B7Y6_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4 5">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_0777</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="4 5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775579.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Y6"/>
    <dbReference type="STRING" id="933801.Ahos_0777"/>
    <dbReference type="GeneID" id="10600236"/>
    <dbReference type="KEGG" id="aho:Ahos_0777"/>
    <dbReference type="eggNOG" id="arCOG00972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108783_0_0_2"/>
    <dbReference type="OrthoDB" id="264480at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="6">
        <location>
            <begin position="5"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE93663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="173" mass="20138" checksum="746E7D1FF09EBE51" modified="2011-06-28" version="1">MLRGIYPGRFQPFHLGHLSVVKWALERVDELIIIVGSAQESHTLNNPFTAGERIEMIRMALKEYGIPTDRYYIIPIPDILMNNVWAYHVKMYVPAFQKVFARNPLVVRLFKEAGVEIDIPPAFNREKYNSTLIRKLIIMNEEWKDLVPSSVYNYIKSIKGDERLKEITGSDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B841</accession>
    <name>F4B841_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HEPN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776780.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B841"/>
    <dbReference type="GeneID" id="10601492"/>
    <dbReference type="KEGG" id="aho:Ahos_1993"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_1_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81593">
        <property type="entry name" value="Nucleotidyltransferase substrate binding subunit/domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="101" mass="11961" checksum="A04FFCD6A557CA6A" modified="2011-06-28" version="1">MSFLLKNSRDFLHVAKRDFEEGLWNLVLFHSEQALQLCVKYKIYLHAGDYPKTHNLNELFSGLSKFEEIDVDTTMLDLLTQSYIRQDIYLIPILKKLLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B850</accession>
    <name>F4B850_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Precorrin-4 c11-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_2002</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B850"/>
    <dbReference type="STRING" id="933801.Ahos_2002"/>
    <dbReference type="KEGG" id="aho:Ahos_2002"/>
    <dbReference type="eggNOG" id="arCOG00645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
    <dbReference type="OrthoDB" id="6633at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF4">
        <property type="entry name" value="COBALT-PRECORRIN-4 C(11)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="4"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="329" mass="36461" checksum="0AECAE0D2831A85D" modified="2011-06-28" version="1">MKGKVAFIGSGPGDPELITVKAKKYIEEADVILYAGSLVNPEILNWAKRDAEIINTAELTHEEIVNIMVKKAKEGKLVARLKSGDFSIYGALNEELQAIKKEGIDYELIPGITAAIAAASSLGIELTLPRVSQTLIITRASASVPMEGSIKDYAPFLNKGASMVIYTGVHIIDKVVEELEEGGLSKDSPIAVVYKATWKGEEKIIKGTLKDIAEKVKKEKITRDAVIVVGKILTPEKYEFKSSAYDENHYTGFRVANQDKLKYFEARYSVDKIFAEEYLKELINALKEGRGNEKELINKMEKVLSEGKINPKLRLEAEEIIAEKKRGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B853</accession>
    <name>F4B853_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiD</name>
        <name evidence="6" type="ordered locus">Ahos_2005</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="5"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776792.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B853"/>
    <dbReference type="STRING" id="933801.Ahos_2005"/>
    <dbReference type="GeneID" id="10601504"/>
    <dbReference type="KEGG" id="aho:Ahos_2005"/>
    <dbReference type="eggNOG" id="arCOG04383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE94877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="347" mass="37169" checksum="C9608DEE6CC4F18B" modified="2011-06-28" version="1">MIQTLKRFGITTGAVAAAASKASVIYLVRGEKPDKVVVPTPIGIRIEIPVEKYLEEEGKKCAEVKKFSGDNPDILDGLEIISCSEFSSSGIQIIGGKGIGVVTRPGLKLEIGEKSISPIAKQMIIDAVSEVTTEGIKITIIVPKGEELAKNTMNPDVGIEGGISILGTTGIEYPVSDEDYLEHIKAEMCVIKASGNNKIVLAPGNTSFEYAKKIYGDIVVKIGDRVGDSIKLAKDEGFSHVVIVSLPGKITKVSAGLLNTHSKFGDARIESIVYSALIAGVPLERLKRIAESLTVSEALEFLTLEERRRVMQIVAKRVLSRLSKFGVKLGVIILSDNGEVLAKEGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>F4B854</accession>
    <name>F4B854_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Precorrin-3B C17-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_2006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B854"/>
    <dbReference type="STRING" id="933801.Ahos_2006"/>
    <dbReference type="KEGG" id="aho:Ahos_2006"/>
    <dbReference type="eggNOG" id="arCOG00647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004423">
        <property type="entry name" value="PRK05765.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="8"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="258" mass="28385" checksum="CCD39B08C276B13B" modified="2011-06-28" version="1">MHVVKMGKIYVVGIGPGGKEARTLEMLEAIKESDVIVAYTTYARLIQDLTDGKEVITAKMKEEIFRAKVAIEKALHGHTVAVVSSGDPQVYGMAGLIYDMACKNNVNVEIQIIPGITAANAVAAKLGSPLSMDFAVISLSDLLIPSEEILNRVRKAAEGDFVIVLYNPINKPLLFEAMRIIKEVKGIDVPVGIVKSAYREDEEIMIATLSTWEKFLNKINMITTIIVGNSKSYICGNKIITPRGYERRYDLHVIDSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>F4B859</accession>
    <name>F4B859_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776798.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B859"/>
    <dbReference type="STRING" id="933801.Ahos_2011"/>
    <dbReference type="GeneID" id="10601510"/>
    <dbReference type="KEGG" id="aho:Ahos_2011"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_898939_0_0_2"/>
    <dbReference type="OrthoDB" id="44271at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-ribbon" evidence="3">
        <location>
            <begin position="4"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AEE94883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="309" mass="33049" checksum="84B1C6E6BAAA7858" modified="2011-06-28" version="1">MVKYCPKCGYPNSDDANFCVRCGYQFSAVGDQQSQQPNSQPPYIPANSNSPESPKKKIPLKAIIGGILAIIIILVVFLVVLPLISPRGVSALASTAQSEFGGKWVIAKCKSGTASYMGNGEYKVKYLNGTVVYDSYNSLQFTNLLSPPFSGSSAYQIIYNVYPSKVVFSIINGTVNSEKTYIILIGAYWNYTPSPASETYSVINSILSNSSYSASISAAEAQAKSQGICFVLSSFNGLDYYYTSTANTTLLSQLPISSSPLQSLGVTSISALGAFEGVSNNEEIAVLAVNLAPTNSQMQSIVSQFQCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8B6</accession>
    <name>F4B8B6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-associated exosome subunit protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Ahos_0829</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775631.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8B6"/>
    <dbReference type="STRING" id="933801.Ahos_0829"/>
    <dbReference type="GeneID" id="10600297"/>
    <dbReference type="KEGG" id="aho:Ahos_0829"/>
    <dbReference type="eggNOG" id="arCOG04187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="OrthoDB" id="84504at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF4">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SDO1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="3">
        <location>
            <begin position="100"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="4">
        <location>
            <begin position="164"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE93715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="232" mass="25923" checksum="AB28A35BA350F895" modified="2011-06-28" version="1">MAKQDYVVIKYESHGERFEILVKPKEAMAFRSGKSISLSDVVISDTIYKDVKKGLKASPSALKKVFGTTDFETVAREILLKGEMPITAEQRKEMLESKKKQLIDFIHRNAIDPKTHLPIPPARIEAAMEEARVQIDLNRDVESQALQIIHELARIIPIKVARATLEIKVPAKYSSKVKSQLSNLGSVKKTNWLNDGTLIAEIEIPAGAQEEVIDKLNSLTKGEVEVKVLQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8E2</accession>
    <name>F4B8E2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl24</name>
        <name evidence="6" type="ordered locus">Ahos_0855</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="4">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775657.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8E2"/>
    <dbReference type="STRING" id="933801.Ahos_0855"/>
    <dbReference type="GeneID" id="10600323"/>
    <dbReference type="KEGG" id="aho:Ahos_0855"/>
    <dbReference type="eggNOG" id="arCOG04094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_2"/>
    <dbReference type="OrthoDB" id="10899at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE93741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="13966" checksum="D07D74E779549257" modified="2011-06-28" version="1">MTSSKPTKQRKMLFSAPYHLRRKMLTAMVSEEITKEYGIKRIEVKKGDTVKVMRGDNVGFEGKVAQVNTKTGRIAIEGLTRKKADGTPVYIWIHASKVMITKLDLSDNKRKASIERKVKQVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8F9</accession>
    <name>F4B8F9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775674.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8F9"/>
    <dbReference type="STRING" id="933801.Ahos_0872"/>
    <dbReference type="GeneID" id="10600340"/>
    <dbReference type="KEGG" id="aho:Ahos_0872"/>
    <dbReference type="eggNOG" id="arCOG04068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190408_0_0_2"/>
    <dbReference type="OrthoDB" id="38650at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049689">
        <property type="entry name" value="CedA1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041797">
        <property type="entry name" value="Ced_CedA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="78" mass="8470" checksum="1C0547D6EF091BB7" modified="2011-06-28" version="1">MSGIESLISNLTTQITETAWSVFILSWAVGWALRGSPIPIFRIKRGGQDIIEDAIIAAFWLAIGSTVFSLISYLAGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8I3</accession>
    <name>F4B8I3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776850.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8I3"/>
    <dbReference type="GeneID" id="10601564"/>
    <dbReference type="KEGG" id="aho:Ahos_2064"/>
    <dbReference type="eggNOG" id="arCOG00505">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061385_0_0_2"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07726">
        <property type="entry name" value="ST1585-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037482">
        <property type="entry name" value="ST1585_MBL-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF4">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE MBLAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="263" mass="29117" checksum="1013E32BD1D125CE" modified="2011-06-28" version="1">MPCTGIHAVPSGPIEFPELVYSFVICDEKVVMIDGGVANSIMDVSFLDKLDYLVITHLHIDHIGLIPDIVSTYKPKILVYEGFKKYLENPDKINKTARQILGELVDIYGEVKPINGDILEVKGGEEIKIGKDTMKIFYTPGHAKHHISVMIGDVLYTGDSAGGRYYGIPFPTTPPPLDLEKYISSLEFQISLKPRVVGLSHGGFTSSSHLLEHLEQIKNNEHVDIDLGGTQGEILRRHLEINYKGLEEARSKAVGNKGLSQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8K7</accession>
    <name>F4B8K7_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Exodeoxyribonuclease III Xth</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Ahos_2088</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776874.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8K7"/>
    <dbReference type="STRING" id="933801.Ahos_2088"/>
    <dbReference type="GeneID" id="10601588"/>
    <dbReference type="KEGG" id="aho:Ahos_2088"/>
    <dbReference type="eggNOG" id="arCOG02207">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_1_3_2"/>
    <dbReference type="OrthoDB" id="146626at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09073">
        <property type="entry name" value="ExoIII_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="9">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="8">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AEE94959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="247" mass="28707" checksum="DB1C66CEF3CF37CF" modified="2011-06-28" version="1">MRLISWNVNGLKAIMSKGFIDIIKSFNADILMFQEIKTDVIPLDLQSLGYEIYVFPAKRKGYSGTMTMTRIHPISVSYGLGKEEYDSEGRVIILEYPEYYVINTYFPNAGEGLKRLDFKLSFNRDFEKFVISLKKPCIICGDFNVAHQEIDIARPKDNVNHAGFTPQEREWFSHFLSLGYVDTYRMFVKEGGHYSWWSYRFHAREKNIGWRIDYCVVSESLKGRVIKADILEKVMGSDHAPILLEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L3</accession>
    <name>F4B8L3_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L3"/>
    <dbReference type="STRING" id="933801.Ahos_2094"/>
    <dbReference type="GeneID" id="71811325"/>
    <dbReference type="KEGG" id="aho:Ahos_2094"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3178368_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="10"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="46" mass="5056" checksum="6117CB3073CF56C1" modified="2011-06-28" version="1">MSFDVALEYLNKAKEYLNASRLLFSNSSYNASSLASEVSAQLAITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8L8</accession>
    <name>F4B8L8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TENA/THI-4 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776885.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8L8"/>
    <dbReference type="STRING" id="933801.Ahos_2099"/>
    <dbReference type="GeneID" id="10601599"/>
    <dbReference type="KEGG" id="aho:Ahos_2099"/>
    <dbReference type="eggNOG" id="arCOG01128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077537_3_0_2"/>
    <dbReference type="OrthoDB" id="85443at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050334">
        <property type="term" value="F:thiaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19365">
        <property type="entry name" value="TenA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027574">
        <property type="entry name" value="Thiaminase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04306">
        <property type="entry name" value="salvage_TenA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="7"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="219" mass="25839" checksum="D32091217264EF80" modified="2011-06-28" version="1">MLTEKMWSAIQDIYNAILSHPFIKGLVNGTLDEEKFKYYILQDYLYLREFSKALAIISAKAEKQDEAMLFSSHLQSIMRVENELHNFFMDTWNISEEEKAKLIPSPTNLLYTSFLLSTVLYKPYFEAVSAVLPCYWIYMEVGKELLKKGSPNHLYDRWIKTYGGEEYERGVRAVLEIVNSFKLTPEEEVTAIRTFRIASIMEYMFWDSAYKLEKFPFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8M3</accession>
    <name>F4B8M3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dual specificity protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776890.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M3"/>
    <dbReference type="STRING" id="933801.Ahos_2104"/>
    <dbReference type="GeneID" id="10601604"/>
    <dbReference type="KEGG" id="aho:Ahos_2104"/>
    <dbReference type="eggNOG" id="arCOG03413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047330_4_1_2"/>
    <dbReference type="OrthoDB" id="117569at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000157">
        <property type="entry name" value="Protein-tyrosine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="76"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="157" mass="17745" checksum="19CC10E7FA52376C" modified="2011-06-28" version="1">MYWVRKGIIGGSCLPYTVDEIKKWKASGVKKVVVLPEEWEIEEAWGSVDYYFSVLEENGLKFIHVPIPDGYPPTLSQFEEIMEWLEGRGNLIHCVGGIGRTGTVLAGYLMVTEGLNANEAVEEVRKYREGAVQTMQQFEFLLSLEGKNNVGKRRLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Q5</accession>
    <name>F4B8Q5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">[LysW]-lysine/[LysW]-ornithine hydrolase</fullName>
            <ecNumber evidence="9">3.5.1.130</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">lysK</name>
        <name evidence="11" type="ordered locus">Ahos_0889</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the release of L-lysine from [LysW]-gamma-L-lysine and the release of L-ornithine from [LysW]-L-ornithine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:48684"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:9715"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:78526"/>
            <dbReference type="EC" id="3.5.1.130"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[amino-group carrier protein]-C-terminal-gamma-(L-ornithyl)-L-glutamate + H2O = [amino-group carrier protein]-C-terminal-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:52676"/>
            <dbReference type="Rhea" id="RHEA-COMP:9693"/>
            <dbReference type="Rhea" id="RHEA-COMP:13328"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:78525"/>
            <dbReference type="ChEBI" id="CHEBI:136763"/>
            <dbReference type="EC" id="3.5.1.132"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-arginine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M20A family. LysK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.130" evidence="9"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775691.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Q5"/>
    <dbReference type="STRING" id="933801.Ahos_0889"/>
    <dbReference type="GeneID" id="10600360"/>
    <dbReference type="KEGG" id="aho:Ahos_0889"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_0_2"/>
    <dbReference type="OrthoDB" id="133929at2157"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00039"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01120">
        <property type="entry name" value="LysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010175">
        <property type="entry name" value="LysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01902">
        <property type="entry name" value="dapE-lys-deAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001747">
        <property type="entry name" value="PRK00466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF28">
        <property type="entry name" value="[LYSW]-LYSINE_[LYSW]-ORNITHINE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="10">
        <location>
            <begin position="156"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AEE93775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="346" mass="38397" checksum="8393BFCF49D52076" modified="2011-06-28" version="1">MQLDKELLLQKAKQYLLELASIYTPSGEEARATSFFEKVSKELNLDLKITKTKSFLLGKGDLLLASHVDTVPGFIEPKEEGGTIYGRGVVDAKGPLISMLLATWILNENGYKVQFAGLSDEENKSSGARELANSGNWYKGIIIGEPTSTTKIVIEYRGVTHVDVICNYESQHSSSANFNPILEVSKKLIEVSQLPTTYDKPSIVPTIIKAGEYVNVTPSTVYLHFDIRYPYGFKLDDILLKLKEEFASCEIKITEQVPPVKVSANDKIVKSLYKALLLQNIKPAFVRKAGTSDMNILSAITKNIATYGPGDSKLEHTSYEKITLEEIFIGITTYTKAIEDLCYSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8W0</accession>
    <name>F4B8W0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xanthine and Co dehydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775746.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8W0"/>
    <dbReference type="STRING" id="933801.Ahos_0944"/>
    <dbReference type="GeneID" id="10600416"/>
    <dbReference type="KEGG" id="aho:Ahos_0944"/>
    <dbReference type="eggNOG" id="arCOG01929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1418746_0_0_2"/>
    <dbReference type="OrthoDB" id="33067at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052698">
        <property type="entry name" value="MoCofactor_Util/Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30388">
        <property type="entry name" value="ALDEHYDE OXIDOREDUCTASE MOLYBDENUM COFACTOR ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30388:SF6">
        <property type="entry name" value="XANTHINE DEHYDROGENASE SUBUNIT A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC Rossmann" evidence="1">
        <location>
            <begin position="39"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="191" mass="21049" checksum="4BCA1597B07EB3C2" modified="2011-06-28" version="1">MKIVIFTSSKEAQMEEPVICDRDTVKIDGSKCIGRSLGIAPYVAKYLKDLGFYVIVIDPFAEDNDYEADEVIKGEVFSIPDELIKGNYVVISTKHVYDTWAIMKSILAGAKAIQVVMSIKRAKVILGKLIKAGISKEELKKLRIPAGLDIGAKTEKEIALSIVTEILAMERGTSGLPLSKVKEFEKVVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Y4</accession>
    <name>F4B8Y4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved pro-fuselloviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776922.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Y4"/>
    <dbReference type="GeneID" id="10601637"/>
    <dbReference type="KEGG" id="aho:Ahos_2136"/>
    <dbReference type="HOGENOM" id="CLU_820394_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="338" mass="38343" checksum="5B2D0BD2FD38DE28" modified="2011-06-28" version="1">MRKSISLLGLGILVLFLLEIAAPPILVKSQSCYDWAISAYCDFTAWCVQLSVANFSWYPINYTSGIYSIYEMLNLPVKADGYICSNGQTFDFNEILIQTALRYWGEYKLVTDIWFFNTETEQYTSIIGLKCINSEYHGKCNYNIWASFSSYGINVWFDVKGNLYNFPLLPLCKGCNQISNYKIRITSMDNETTPITFNGEKFDPSVSIEVHSACRCIFAEKDPYFFIVFMLYVNGSAICVLQPHKICQLTSASSESESANCDAEIGLYQPPNGVAYGSIEYCKLICHYCLRVCEYVMGMKKGICSVFGFLPNYLTCHSPESCGYTKHNNNIASRTSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B8Z6</accession>
    <name>F4B8Z6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8Z6"/>
    <dbReference type="STRING" id="933801.Ahos_2148"/>
    <dbReference type="KEGG" id="aho:Ahos_2148"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066799_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF20">
        <property type="entry name" value="HYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="332" mass="37012" checksum="449CEF3F7C317ABF" modified="2011-06-28" version="1">MELAIVGGGPAGLSLAYFLRGSKINATVYEGLSDVGLKPCAWGVMKGIEDYLPIPKEAIISEIKGFRIYLDGKLLYDIKGREILGYIVNKPKFLRILGEKVDLRLNSKVIEKDGKILVNDKEIEADKVIYANGHYSFKGEYAIPAIQYITDYKIDPEIVEMYFFSDLLGYGWVFPEEDGSKIGIGGYADVNFLKERLKSLVKGKIKAFQGAKVADYGVIEDRLSNGNYIGEALGTVYAVTGEGIRPSIISAKIMADSLLNNKDFSKEFKKSKLYWSLQIHAKVIRDSKKKGTQSIKGLERVLLKTPPELVLKFALGDFTKIDLLKIFGRMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B939</accession>
    <name>F4B939_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_2191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776977.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B939"/>
    <dbReference type="STRING" id="933801.Ahos_2191"/>
    <dbReference type="GeneID" id="10601694"/>
    <dbReference type="KEGG" id="aho:Ahos_2191"/>
    <dbReference type="eggNOG" id="arCOG07320">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_374933_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE95062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="756" mass="85740" checksum="DE3C1F1F3E6449D5" modified="2011-06-28" version="1">MIKLLLDSSIFSRAIITFDISSLKKFMDVDEKEVVVGNLTSMSGEILSYLNPKPIKAATKLPDGSYYKIIGKKYGMTYAEVPAGLTFEELIEKLKSENLFPAIFPLYLKGTIGGFISTNGSGFGSYKYGFVKYKIPVYELKDKDTAIVGLVNYSELIELDQESPFAWSAVIFPTENVVKYYVPSFYSSLVGKGKVVSLEKLINDIYNSVKSILKRDYIPVCLRASEYSVLIQSPIESKLGYIINYNSPSKFYVICGNIKRDNLDKLFDFLKKNPSVYPFPSLQEYKEIHKFILSKYKKEIKVPKNLEKIKSQYLDAVQCINCGLCLDKCLAYTVTKNIIYSPVGKINRLLTGVMDFEYCFGCKNDDDYCPVGIKISELTEVLPQLNKYKDKISIPIDTVPSRLKELEALLDSKYKSYPVYLLFVGCAAKYDPLGVEGFMNFLLEKGEELKGFSPRVKLLDNECCGFDKYISGDIDGANSDVSKIVDIKNKLGAQKVYFLCPEGLYVYNSLSGDKGILAYEVIKPYVKDKVYLGCWAKKLGFEGEEHECAGLLFTSYKGAQLPLFKKNIITICPFSTWKFSTQSVYSLFNKGEKLKAKDKVIFEFSDQLFTDYVISSLKNSVLASVDDIADKTINWIMGGKNYFILLIIPIIRKRFSSLLISSLSENKELVNSLKTLSENKLLMEDKIRKVIDIINSYNFTELVSDLIVRISNSPRLEYEAKKIIESEDYKKALEEAIKKVIIDRVLEDTIQEVIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B946</accession>
    <name>F4B946_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_2198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776984.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B946"/>
    <dbReference type="GeneID" id="10601702"/>
    <dbReference type="KEGG" id="aho:Ahos_2198"/>
    <dbReference type="eggNOG" id="arCOG04165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151699_0_0_2"/>
    <dbReference type="OrthoDB" id="33373at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE95069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="101" mass="11725" checksum="9170B7C1436FFA02" modified="2011-06-28" version="1">MPTIHLSIPEGMYEELRKKAEDMGIQITDLVKFYIRQGMEKEEEGENVQKNTECEENIAFLEAKVAQLDAIVGELVRKLKNIEDEDIGEEEVELKEREENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B970</accession>
    <name>F4B970_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved secretion protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_0977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="214" mass="23584" checksum="B2D3C859484C4DA1" modified="2011-06-28" version="1">MLGKDFQKYWAGPEEKNGIVSGIKGAFKPKEPLKYRLVQAHYQISSMISRLDAYISRMQERDKTLFERVVEAQMAKDTARAAMYANEVAEVRKITKQLIMTQIALEQVELRLETVSELGDVFVNLVPVVGVINELRSVIRGVMPELSVELGELGEGLQEVVIEAGDFTGASGISAAPTAEARKILEEAAAVAEQKMKENFPELPLSSSTISQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B988</accession>
    <name>F4B988_ACIHW</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B988"/>
    <dbReference type="STRING" id="933801.Ahos_0995"/>
    <dbReference type="GeneID" id="10600468"/>
    <dbReference type="KEGG" id="aho:Ahos_0995"/>
    <dbReference type="eggNOG" id="arCOG01403">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glycogen Phosphorylase B"/>
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    </dbReference>
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        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="165"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="348" mass="40400" checksum="291F39F6975C3D73" modified="2011-06-28" version="1">MDIIFINHRDPFHPNAGGAENVIYEIGKRLKEVTWLSEKVKGRPDSEYIDGIKIIRKGNKFTLHFYSLIEAKRHEIVIDSVAHAVPFFSYLVNDKAIALIHHVHQDVVKFELNPLLAEAVRLAERRIRNYKYIISVSNTTKQDLIKKLGIDENKITVIYNGVDHNKFNPGIKSEIPIVFWIGRMMKYKNPFDIIEIKRRIKNKNVKFIVAGGGELSTEFREVAKEEGIQYLGKIDEKTKIELYRKSWVILSTSFIEGWGMTIVEANSCGTPAIAYSTGSLPEIIKQGINGFVVPYKDVNTMARYIDYITEDENIMKELSRKSYEESLKYDWNKTAEEYRKYIENIYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B989</accession>
    <name>F4B989_ACIHW</name>
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    <organism evidence="1 2">
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
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                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B989"/>
    <dbReference type="STRING" id="933801.Ahos_0996"/>
    <dbReference type="GeneID" id="10600469"/>
    <dbReference type="KEGG" id="aho:Ahos_0996"/>
    <dbReference type="eggNOG" id="arCOG02004">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302564_0_0_2"/>
    <dbReference type="OrthoDB" id="38446at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
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    </dbReference>
    <dbReference type="GO" id="GO:0016853">
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
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    <dbReference type="InterPro" id="IPR026286">
        <property type="entry name" value="MaiA/AMDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40267:SF1">
        <property type="entry name" value="BLR3294 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17645">
        <property type="entry name" value="Amdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="211" mass="23654" checksum="A8D9277167590376" modified="2011-06-28" version="1">MISLILSEENPTLPNDIKLVFNDVKVFYMKYYPGHGIRKDEQERMYKELSLIKNEVGKGDVIVYARSYGVFYRDGMNRLKKFFSKPVVISTEAIIDRLRELGAKKVYVVTPYNQRRHDYEIKWIQGFGFQVVGSIALGRTGGPAIASTPHELVINAVKIAQESSADAIYVACTILSTLPILDKLSGKLPVVTAASATVDKVKEIINLRNNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9A8</accession>
    <name>F4B9A8_ACIHW</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
    <gene>
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    <organism evidence="1 2">
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            <taxon>Thermoprotei</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="42860at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="440" mass="49067" checksum="815CAFC0882AD352" modified="2011-06-28" version="1">MKGLSAVIGMVFLLIVMIAVLVPLALTVTSTTSKEEENINAIQPLQAEVEEQISEVNTENSPIGFYYCNVTGKAVLVYYSSPPIPLNVSYFLGYKGGYIQIIDPQPTETTWNKYPALEYQVGSFNKLAMVTTLGNIIYADPMPHSQESNNSSSAYDPYWVYRLKTPYAAYTNPSTVQICDNPSAITNDWQPCGINNAVLIVLKQEHKPLCINLTIHFSCYFATGFRHTGDLKTNIGIYSGSFYGQIEFGNYFLGANSGVWPFEGFQCILLNISHGYVSTFAPYGVGLGVLASCSHLVYKTRCAWGSVVGFNYLLSIHINCCCQIYIELYKECNGKYVPVKLPYITPEYIKFCWCYCWLKPLPKYEGNYNETEWGHMRCYMCVPKSTSCVVPFGEIKAAVKVVGYTACGFCPVEYPYDPIGVAVEIIWGESVYVSGIQIAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9E4</accession>
    <name>F4B9E4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ZPR1-related zinc finger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Ahos_2219</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777005.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9E4"/>
    <dbReference type="STRING" id="933801.Ahos_2219"/>
    <dbReference type="GeneID" id="10601724"/>
    <dbReference type="KEGG" id="aho:Ahos_2219"/>
    <dbReference type="eggNOG" id="arCOG04265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107446_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="ZPR1-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AEE95090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="172" mass="19582" checksum="7B0FE6A8C95A22AC" modified="2011-06-28" version="1">MEPKLIFEAKLKCPVCGNETLIARDYLYDAGEVGKLVLSNWECTSCNYKFRDVKPYETREPKTLEFVVEKEEDLNVMVYRSPFAIVKIPELGIEIYPEDASQGILSTVEGILEDFLENLGSLCQENDCYDVYEAKEGKRKFTLIIEDSSGLSFIKSEKVKVTRSLSLSQTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9I9</accession>
    <name>F4B9I9_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12 13">Pyruvate kinase</fullName>
            <ecNumber evidence="3 12 13">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Ahos_2264</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="3 12 13"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777050.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9I9"/>
    <dbReference type="STRING" id="933801.Ahos_2264"/>
    <dbReference type="GeneID" id="10601770"/>
    <dbReference type="KEGG" id="aho:Ahos_2264"/>
    <dbReference type="eggNOG" id="arCOG04120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_2"/>
    <dbReference type="OrthoDB" id="56298at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="14">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="15">
        <location>
            <begin position="329"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AEE95135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="445" mass="48764" checksum="7E55460F79AA082E" modified="2011-06-28" version="1">MRKTKIIATLGPSSIDKVKELSQYVDIFRINFAHGDTKSHKEYFEKIKEYSDASILVDLPGPKIRVGDIKGKIVLKPGDKVVFSPYEGIPVEDPLFYSGVKENSYVLIADGSIKINVTKVEKDRVEGVVIEGGTVTSRKGINIPDIKIPSGVTDNDLQLLKEALDLGADFIGLSFVLSKDDVLKVKGISKDRAWIIAKIEKKNAVENLKDIIKVADGVMVARGDLGVEIGLENLPFTQKKIIRLSKLYGKPVILATQVLESMVNNPIPTRAEVIDISNSVSEGVDAIMLSDETAAGNYPVEAVKTLHDIILAVEKRVKTVRPPPLTGDDAIAVASVNVAEISKAKLLVVHSRSGTSIIRISRLRPKVPIIGLSPNKELIRKLKICWGVYPFLTEKSLITLNDIIAYAEEISKKFAKCNENIVITGGDPTLEEGRTDFIKLHQIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9J2</accession>
    <name>F4B9J2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777053.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9J2"/>
    <dbReference type="STRING" id="933801.Ahos_2267"/>
    <dbReference type="GeneID" id="10601773"/>
    <dbReference type="KEGG" id="aho:Ahos_2267"/>
    <dbReference type="eggNOG" id="arCOG03852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302565_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="25358" checksum="000A317D65E6D14E" modified="2011-06-28" version="1">MDKLKLKDLKSPKQEIRQKAWEEVINIIKSGYYSNLLENRGFFRSLLWFPLQGVRDDAWNHLEVYKMLTIEGIERTLVANSDKIKISAWEHVEELLKYELVPKDIIISSRYSFWRLLRSYYPTIRKKAWKLFPKLVELGIIQPSDKDRYYEFLRHKKPSVRIYAWKYSLELIKQGFITKENILNQIKYLEELSTKESNIKKIAVKILSELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9K3</accession>
    <name>F4B9K3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal-transduction protein with CBS domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Ahos_2278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Prangishvili D."/>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
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                <person name="Prangishvili D."/>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
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        <property type="project" value="TreeGrafter"/>
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</entry>
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    <gene>
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    <organism evidence="1 2">
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        <lineage>
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            <taxon>Thermoprotei</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4BA39"/>
    <dbReference type="STRING" id="933801.Ahos_1152"/>
    <dbReference type="KEGG" id="aho:Ahos_1152"/>
    <dbReference type="eggNOG" id="arCOG05993">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117490_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="217" mass="25552" checksum="1749626D4527C4C7" modified="2011-06-28" version="1">MQYETLGKKVEINLKIFCTNYYVFLERLLDKKSLVISYYYSVNLTLLMNFASLYYRKTGKSICIGNFGKIKWEFYPKDVDFPIKCDENSAFLVFEAENENELPEKFIFATSYKNLKVNAIKVRVEKFEYNTYKAIIEDEIIPFKIVKGEINEIKLANDYTELLNIIKELGGECEMRDLVNIASKRLNIPRDEVKEKILFLKEIDKIDIEGKKIKLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>F4BA41</accession>
    <name>F4BA41_ACIHW</name>
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        <submittedName>
            <fullName evidence="5">ATP/cobalamin adenosyltransferase</fullName>
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            <taxon>Acidianus</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="aho:Ahos_1154"/>
    <dbReference type="eggNOG" id="arCOG00489">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AEE94038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="174" mass="20052" checksum="FB4F54E9427A01EE" modified="2011-06-28" version="1">MFTRTGDDGNTNVINKRVGKDSPLVNMLGDLDEVNSFLGLALAKLPWDDMKKDIEKIQYELFAIGEELSTGNIKITQENVKWLEERTVAYRKESGPVKLFVIPGGSEEASYLHVARSVVRRVERNAVKYSKEIEFDKWIIVYLNRLSSLLFSMAIVANKRKGIEERIYDIRKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3P0</accession>
    <name>F4B3P0_ACIHW</name>
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        <submittedName>
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    <gene>
        <name evidence="3" type="ordered locus">Ahos_0034</name>
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    <organism evidence="3 4">
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            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE92929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013774846.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3P0"/>
    <dbReference type="STRING" id="933801.Ahos_0034"/>
    <dbReference type="GeneID" id="10599474"/>
    <dbReference type="KEGG" id="aho:Ahos_0034"/>
    <dbReference type="eggNOG" id="arCOG01238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_498421_0_0_2"/>
    <dbReference type="OrthoDB" id="28065at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00919">
        <property type="entry name" value="Heme_Cu_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="entry name" value="Cytochrome c oxidase-like, subunit I domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF18">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="Cytochrome c oxidase subunit I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome oxidase subunit I profile" evidence="2">
        <location>
            <begin position="25"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE92929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="537" mass="59942" checksum="71199F3878899B43" modified="2011-06-28" version="1">MSSKSNPLVTLINAVFQLDKDWVSRVSMAMIVMSLIWGILGIIDALMARIQEMVWGLSQTLVFTSQEYYGGITLHGVRDLFGFAVQLEVAIFIFLSYKLLNFQPRAKWFLNIGFILFNIAFMLMEGPIVAFPTFNDNYFGATGWYYLNPLGIPNYSEYVVSPLWYIGYELMDIGVYIYVIWLIYHYYFVSRNMKEKLPIFAVFALMTSLMIAIGWSGETAANTWDILAYFGLVGLDPIANQIAFWILGHSIVYIVWMPAVASMYYLIPLLAGKPLYSDKMGRVAALLYLIFSNNVPIHHLYMVDLPVSVKIMQEVLTYAVVVPSMMTFFNLWATVKGAQFKPNLISVWISISFAGAIAAGVTGIANANISFDSIIHNSMWVPGHFHAMIFWSIVPAGFATLYYMVPLLTGRMWYSTKLGWIHMVGYMIGTAMVVVGFDALGLAGLIRRAEIYPIIPAYVTPEVVASVGAMIADFATLIWLGNLVITLLKGRSANVEGLSVSDTVTTIAMQLGYEGPSLNEISNKLTTAISRIVKIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3U7</accession>
    <name>F4B3U7_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps27e</name>
        <name evidence="7" type="ordered locus">Ahos_1251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5 6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776050.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3U7"/>
    <dbReference type="STRING" id="933801.Ahos_1251"/>
    <dbReference type="GeneID" id="10600730"/>
    <dbReference type="KEGG" id="aho:Ahos_1251"/>
    <dbReference type="eggNOG" id="arCOG04108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="OrthoDB" id="5718at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="65" mass="7064" checksum="127872ECA24EC97D" modified="2011-06-28" version="1">MKKTKILIPEPKGKFIRVKCTNCGNEQVIFSHATFPVRCLACGTQLAYPTGGKAKIVGETIKELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B3W2</accession>
    <name>F4B3W2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ferripyochelin binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776065.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B3W2"/>
    <dbReference type="STRING" id="933801.Ahos_1266"/>
    <dbReference type="GeneID" id="10600747"/>
    <dbReference type="KEGG" id="aho:Ahos_1266"/>
    <dbReference type="eggNOG" id="arCOG01849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_2"/>
    <dbReference type="OrthoDB" id="10940at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050484">
        <property type="entry name" value="Transf_Hexapept/Carb_Anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061">
        <property type="entry name" value="DYNACTIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13061:SF29">
        <property type="entry name" value="GAMMA CARBONIC ANHYDRASE-LIKE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="171" mass="18698" checksum="CB06BDDDC340F056" modified="2011-06-28" version="1">MPIEDFMGRKPRISKNVFLHPTAYIIGDVEIGEMSSVWHYVVIRGDNDSISIGKESNIQENSTIHTDPGFKVEIGDKVTVGHNAVIHGAKVSSNVIIGIGSILLNGSKVGEYSIVGAGSVVPPNAEIPPYSVAMGIPAKVVRKIREEEIKMIEDNAYEYVLHVKRFLNNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4K5</accession>
    <name>F4B4K5_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trpD</name>
        <name evidence="7" type="ordered locus">Ahos_1352</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="4"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776150.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4K5"/>
    <dbReference type="STRING" id="933801.Ahos_1352"/>
    <dbReference type="GeneID" id="10600838"/>
    <dbReference type="KEGG" id="aho:Ahos_1352"/>
    <dbReference type="eggNOG" id="arCOG02012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_2"/>
    <dbReference type="OrthoDB" id="8214at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2 4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="5">
        <location>
            <begin position="72"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="106"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="346" mass="37916" checksum="91A3E16727911163" modified="2011-06-28" version="1">MNFKEILEKITDRQDLSTNEAELIANSIMKGEIPELVSAAILSSLKTKGEAPEEIIGFVRSMRANAVKVDLGDVVDTAGTGGDGLGTINVSTATAIVISQVCRVAKHGNRAASSKSGSADFLETIGYNINVTPEKAKELISKDNFVFLFAQLYHPAMKNVATVRKTLGIRTIFNFLGPLTNPAMAKKQVMGIFSKKFMDKIATAALSLSYDKLLIIHGEPGMDEVSPLDVTTIYEIKGNNIDKYEFNFAEIIKYKPKISELLANDSKESVIKVLRAFAGKDKAVEEFIKVNSAVALYSCDRVKDFKDGYEYSSQLISTSLNKLTDIISHNGNLNIFKNFMAMISDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4Q3</accession>
    <name>F4B4Q3_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthine synthase</fullName>
            <ecNumber evidence="6">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dphB</name>
        <name evidence="9" type="ordered locus">Ahos_1400</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="6"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776198.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4Q3"/>
    <dbReference type="STRING" id="933801.Ahos_1400"/>
    <dbReference type="GeneID" id="10600889"/>
    <dbReference type="KEGG" id="aho:Ahos_1400"/>
    <dbReference type="eggNOG" id="arCOG04161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_0_0_2"/>
    <dbReference type="OrthoDB" id="39139at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="252" mass="28232" checksum="659872352BF0E82C" modified="2011-06-28" version="1">MLYFIGLGLAKKFLTQASIEAMRKVDVLYLDAYTSLSCDINKEYLEKLLGRQIILADRSTLENNSKNIIKLLKEGKSVGIATIGDPMIATTHVSLATEAKNKGYNVIIIPGISVHCYIISKSMLSSYKFGRSVTVVYPYDKILDTTPYEVIKDNSSRGLHTILYLDLKEGKPMSAKDAISLLLQMEEIKKEGIITPNTQIIIGQRLGCEDEEVKALTISEALEYKYKDTPHIIIVPSKNLHYMEVEALKCLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B4V4</accession>
    <name>F4B4V4_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_0227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
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            <taxon>Sulfolobaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775036.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B4V4"/>
    <dbReference type="GeneID" id="10599667"/>
    <dbReference type="KEGG" id="aho:Ahos_0227"/>
    <dbReference type="eggNOG" id="arCOG00625">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2009978_0_0_2"/>
    <dbReference type="OrthoDB" id="42807at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
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        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="64"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE93119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="123" mass="13760" checksum="36BA6E6247711EC3" modified="2011-06-28" version="1">MALKVKLKHKNVLTLNGEEVVKNVKPLISKCQVIVVTEGNKIIGYLTGDEIEKANGEEKLKDIIKKGKSILTITGKEDPLEILNLMVRSHIDYLIIVDEKGRVKGALSIDDALENVKRMIKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B553</accession>
    <name>F4B553_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Antibiotic transport-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Ahos_1472</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776270.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B553"/>
    <dbReference type="STRING" id="933801.Ahos_1472"/>
    <dbReference type="GeneID" id="10600971"/>
    <dbReference type="KEGG" id="aho:Ahos_1472"/>
    <dbReference type="eggNOG" id="arCOG02175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018329_0_0_2"/>
    <dbReference type="OrthoDB" id="42357at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050545">
        <property type="entry name" value="Mycobact_MmpL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406">
        <property type="entry name" value="MEMBRANE PROTEIN MJ1562-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33406:SF6">
        <property type="entry name" value="MEMBRANE PROTEIN YDGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="626"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="653"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="696"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="726"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="266"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="624"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AEE94355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="766" mass="84297" checksum="77ECB361F08797EC" modified="2011-06-28" version="1">MNRKLVIVLWIIGILIAVLLSSQSSNYLNYNENTTIPSSYPAAKAQELLSEYFHGGNVNNSIDVVLINSTPQEVYKAVKIIDEIDGVCKTESIINAYLAYDEILGKEINYSGYKIIEENKGNISSQELKEEISQYLHIPIYYTCLFNVSPQNLLTRNETVFFLITPPAQFTSTYEAKNVSVIFVYTKYCPNYNFKNGTYPDGVISCQIQSKLSKCGFNNFYLTGPAPLVQELSSSESQRQAITFALVFIALLLITGIYFRSVIAPIITLSIIALSVIFGMAIVTLVGKFYHPVDFQVIEPMISVLLGIGTDYSVFLLSRFKEELSKGRNKEEAMEISVKTSGKAILISGSAVTLVFLSLSFIPYLHTWGLTIGFSVPITVLIAVSLLPIIYGKIGSKIFWPSHLSFSENKSLGNIARITMRKPKTTLAISVIIGLIALAFILSVPLSLDFTSGLPNIPAVKGLKILENAFGQSFVNPVLIVFNESKTVMNSTYVNTSLLIQIAKIEENISKLSGVKQLIGPVPCNFNGTITPEILHQIRENLGTNNKTLLVTVIMNYGTYTQQAQKLVLTIQKIVNPYHGYVGGTTASVIDALDYLLPYYEMLIIILPIILVISLAVFLRSIKISIGAVGTILLSITLSLAIIYLFFRSANGILFFIPITIFVLMIGLGNDYSIFILTRVKEEIEKERSIESIVRAISISAGAVTALGVILAASFGVLAIDPIKPIAELGIGIAIAALLDTFIIRIFIYPAILKIALKLNETIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B5X5</accession>
    <name>F4B5X5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved pro-lipothrixviral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B5X5"/>
    <dbReference type="KEGG" id="aho:Ahos_1591"/>
    <dbReference type="eggNOG" id="arCOG00724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1709222_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="155" mass="17971" checksum="A8ED93E1759AD7D5" modified="2011-06-28" version="1">MGILKIAKDLGVSVSATKLQKLVFLIEIELNFNLGYEFIPYYFGPFSKELQDDVYRLKNLGYVEVKKEAVEDFVSGAIIGFKKAYKISDKVTFDFSSLDKNFVEFVVTHLKIPLDDLLKYVYVKYPEYTVNSVIKDRVFKAKFSCPSLLNFFWNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>F4B608</accession>
    <name>F4B608_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_1628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776421.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B608"/>
    <dbReference type="STRING" id="933801.Ahos_1628"/>
    <dbReference type="GeneID" id="10601128"/>
    <dbReference type="KEGG" id="aho:Ahos_1628"/>
    <dbReference type="eggNOG" id="arCOG02687">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_2"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="33"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE94506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="473" mass="51251" checksum="EC1112DD2CB04925" modified="2011-06-28" version="1">MADKNSPQKNKNIAAQLTARIDRLPVMVLPLSVVLSLAFGYFIALYDVIDVGIAFSATSLKYTGLTSTEASIVVSLGLFGYIPGALLLGYLGDRIGRKPMLMTTAMLTAIGSLGNAFSSNFVEFSIFRFITGMGIGGDLILVPTYLVEMVPAKNRAKYFNLVYIAGWAGLGLGPFMASYIDVANPAIGWRVIFLVGATLAFIVFAIRGHASETVRMLALKGKIDEAEKVVKDMEEKAMKKTGLTTLPEPIVYTFEHKKVDPFYVFKNPKYRVRVIAVMLSIFFFYFGEYPYLTEFLLWTSTVPSLSSLSNQITLYYGDAGIATFIGAILLRTVIDKIRRPILTTISYFVGMVLGVAIAVIGATSANLTMMLTGFVLANLIGVGWSNQLNYLNGTENVPTYARATSFAFSDGVAHLGAAISTAIILSIIPVLGAVTTWIGFQIPMVIMGIILIFVLPNTIGKSLEEINEATAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>F4B698</accession>
    <name>F4B698_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Ahos_0498</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B698"/>
    <dbReference type="STRING" id="933801.Ahos_0498"/>
    <dbReference type="KEGG" id="aho:Ahos_0498"/>
    <dbReference type="eggNOG" id="arCOG00905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044815_1_3_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10030">
        <property type="entry name" value="UDG-F4_TTUDGA_SPO1dp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000013">
        <property type="entry name" value="Type-4 uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053423">
        <property type="entry name" value="Type-4_UDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051536">
        <property type="entry name" value="UDG_Type-4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040953">
        <property type="entry name" value="Arch_udg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693:SF1">
        <property type="entry name" value="TYPE-4 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33693">
        <property type="entry name" value="TYPE-5 URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="12">
        <location>
            <begin position="27"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AEE93386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="207" mass="23058" checksum="C6355B1123582E87" modified="2011-06-28" version="1">MSLEEIAKQVRECTKCKLHLTRKNAVPGEGNPSAEIMLIGEAPGANEDEEGRPFVGAAGKLLTELLSSIGLNRKDVFITNLVKCRPPNNRDPEEDEIIACSPYLDEQIRIIKPKIIVTLGKHSTTYILGKIGIKVKSISAVRGKFFTWNYGNVTISVFPTYHPAAALYNPSLRDELFSDFKKISERLNSKVFTLDYFLEKNGSGNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6D0</accession>
    <name>F4B6D0_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">VapB-type antitoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1673</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6D0"/>
    <dbReference type="KEGG" id="aho:Ahos_1673"/>
    <dbReference type="eggNOG" id="arCOG02217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175270_2_0_2"/>
    <dbReference type="OrthoDB" id="45710at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039709">
        <property type="entry name" value="VapB3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42244:SF2">
        <property type="entry name" value="ANTITOXIN VAPB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE94550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="70" mass="8499" checksum="ABD2B9755DB20B9B" modified="2011-06-28" version="1">MSTVYSIRIPKKLRELMNSVNVDWQREISKYIEDRVRKELIRKYIEEGKNNLTKMKNINNSDLIREDRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6E8</accession>
    <name>F4B6E8_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CoB--CoM heterodisulfide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_1691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776483.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6E8"/>
    <dbReference type="STRING" id="933801.Ahos_1691"/>
    <dbReference type="GeneID" id="10601191"/>
    <dbReference type="KEGG" id="aho:Ahos_1691"/>
    <dbReference type="eggNOG" id="arCOG00338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="OrthoDB" id="144689at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051278">
        <property type="entry name" value="HdrB/HdrD_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947:SF1">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="10"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Cysteine-rich" evidence="2">
        <location>
            <begin position="159"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="305" mass="33925" checksum="C840C69B9DA513B8" modified="2011-06-28" version="1">MSTNNPYGKVAFYPGCALDGLGKSYDVSLRLVAEDLGLKFERLDDYNCCGALEVKNVNTMAGLLLPARNLSLARKIGANAIMSACPGCHYSLSRTQYYMSKYTKVRDKVNAYLEKMGEKPYDLQLMTIHAVEYFYNTVGVEGIKAKVKRPLTGLKVAPYYGCLYTRPKPYTLAGYMKLKDDPERPIFMDELLKALGAEVVPFEAKTMCCGGPHVYSDVEVALHLEARILKEAKRNGADMLVVDCPLGGVALETNMNKIAEKYGEDLRMPIVYFTQLMAFAFGHSPEEALLTANLTNPMSILKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6M9</accession>
    <name>F4B6M9_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775355.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6M9"/>
    <dbReference type="STRING" id="933801.Ahos_0551"/>
    <dbReference type="GeneID" id="10599994"/>
    <dbReference type="KEGG" id="aho:Ahos_0551"/>
    <dbReference type="eggNOG" id="arCOG01776">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207639_0_0_2"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="86"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="231" mass="26505" checksum="5EA1FA819C24E585" modified="2011-06-28" version="1">MRFICPYDGTPVNENLECEKGHKFIYHDGMYDFLVNSKVKGDALLERIAPIYDKIWAPLGFFLTSFYSYDRIFKDAGSFLSSRNFLDIGTGPGTIFKYVKCEYCIGLDISTKFLLILKKKFSHVIAVRADATSLPIESASMDSVSSFLVLHMLNNPSLAIKEISRVLKPNGKCEILVLVKSNVISRLLSKWWKIELRHRDYYISAIEENKLTLLEIKKYGPWLLLKCKKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6S3</accession>
    <name>F4B6S3_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Second ORF in transposon ISC1058</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B6S3"/>
    <dbReference type="KEGG" id="aho:Ahos_1739"/>
    <dbReference type="eggNOG" id="arCOG02754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176753_0_0_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="75" mass="8840" checksum="AFD6E519FE26DBC1" modified="2011-06-28" version="1">MNIKFPEANDELEADGNKHKQGRTMHYCKMEKNKDLKFLKIYKDQFNVINAEVTSNEVKTAVKVLQDKGKKILWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>F4B6T2</accession>
    <name>F4B6T2_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CRISPR-associated protein, Csa1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_1748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
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                <person name="Jiang C.Y."/>
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            <strain evidence="2 3">W1</strain>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
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            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE94625.1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="283" mass="32147" checksum="15997EB49E21A18E" modified="2011-06-28" version="1">MYNVRSQIIRQVRRLHSYRASDPIEEELRGWNYHMPPIKPRRYLGLSMSDVAYRYCDTKRDVYLRRVLKVQGEQRSALVLGQSVHEVIASVSNEIVKLLSLGYKPWAAIEAIFRKANKVSNLCPQQFFKFCEEVYKAFALDLISDFSDSTSFLPIISEFRVDGTPLGLSSKLSVDAITQLSLVVEIKVASTQEFHKLALAGYALALESALELPIDFGSLIYVNGINKNTPEFKYEVYYISPDLRKEFIDARDEVIDMVSEGRDPGMPTACCPSCPFLNYCNKG</sequence>
</entry>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
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            </authorList>
            <dbReference type="PubMed" id="21607549"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
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        </source>
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    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="76" mass="9001" checksum="9A40495440284577" modified="2011-06-28" version="1">MWREVPLTHIILEKLRKLEGPIKDDDLYNDVRKSVDYEVPFGDFLRALMSLEIRGYISVSLIRENSRMITYIGDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7I5</accession>
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        <submittedName>
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        <lineage>
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    </organism>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
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    <dbReference type="AlphaFoldDB" id="F4B7I5"/>
    <dbReference type="STRING" id="933801.Ahos_0703"/>
    <dbReference type="GeneID" id="70682581"/>
    <dbReference type="KEGG" id="aho:Ahos_0703"/>
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    </dbReference>
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        <property type="entry name" value="Ribbon_hlx_hlx"/>
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        <property type="entry name" value="BLL4998 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="48" mass="5650" checksum="B427CBB5F864DACB" modified="2011-06-28" version="1">MKIITVKLPEQFLEAIDELVNTGRYESRSEVIRAAIGDFIRKELWIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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    <dbReference type="AlphaFoldDB" id="F4B7I8"/>
    <dbReference type="STRING" id="933801.Ahos_0706"/>
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    <dbReference type="KEGG" id="aho:Ahos_0706"/>
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            <begin position="1"/>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AEE93593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="288" mass="31982" checksum="51FACB1F3A01C5FA" modified="2011-06-28" version="1">MLISLTYLKLKEMSRKHNIEKAKKLIKTAKERGAKLIILPSLFPVGNTFEIYNNEKKMRSVVKNLAEKIPDNSTDILVKLAMEGEIHLIAGPLLEQAGPKIFLTTLVISPDGEIIGKYRKIMMSEKDVKLGISGGKEPIYVVLDKKYGVLSEDDLFSPEISRILALGGSQLIIGTTRPVNKKQDLIKYVAITRSVENGISYLINGEMIENEDGEIVGYTPTFIATPDSLVYKEAEDEDSIVLVESSMITQNKEGVNTRLNGIESIIYGLCKSIKKTKMNEKPIPKPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7N0</accession>
    <name>F4B7N0_ACIHW</name>
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        <recommendedName>
            <fullName evidence="1">HEPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
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            <taxon>Thermoprotei</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AEE94777.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="5"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE94777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="113" mass="13147" checksum="C0F43BF2532A4ABC" modified="2011-06-28" version="1">MSELIEKGKQSFTTAEDAINKGYYWLACFLFHQAVELAIKGLLEITRNEHPYTHNLVELLKYFNPPDDILTCAMNLNPHYISSRYSLMSFYSRNDALNCKKNAEAILKWLKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7R4</accession>
    <name>F4B7R4_ACIHW</name>
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        <submittedName>
            <fullName evidence="7">Glycosyl transferase family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Ahos_1938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE94811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776726.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7R4"/>
    <dbReference type="STRING" id="933801.Ahos_1938"/>
    <dbReference type="GeneID" id="10601437"/>
    <dbReference type="KEGG" id="aho:Ahos_1938"/>
    <dbReference type="eggNOG" id="arCOG01389">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:hyaluronan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085029">
        <property type="term" value="P:extracellular matrix assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030213">
        <property type="term" value="P:hyaluronan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913">
        <property type="entry name" value="HYALURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22913:SF12">
        <property type="entry name" value="MANNURONAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13641">
        <property type="entry name" value="Glyco_tranf_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AEE94811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="433" mass="50085" checksum="C0EDA55029FAEC88" modified="2011-06-28" version="1">MRFDIEIYLITSTLVSAFTFIYITSTLLFSLIKKGKSSPPLEVSENVTAVIPVYKEDVKLFRKVIQSVKREKISFIVVGDGATEPYKSIVESEGGIFISIKKGGKRKAIKEGIKLVNTPYVLLLDSDTILPQGGIKRLISRLQGKVVAVSPEIKLVRGKSRLAYYSSEVVHFLRRLSYKALSIFGSVLTLNGQCILAKTEVIKPFILSKEYDEVRLFRFSTILGDDRQLTNYVYKLGFKAEIVDDVIVKTKAPDSISAFFKQLIRWYRANNFFMIKEFFDGSIFKKGAFYSFSLVYWNTLPILIIISNLTFIEIMLRHILSHPKLLDRIIEDPIHAFYFIVVRRIENFMHLFYVDPSFIYRHVDPFYFEIFMLHNVFAELSSLVSSIMLLIVFAHFRKNIRGFIMGLAAFPLMFFAEIIAMFTMWKKKWEGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7X6</accession>
    <name>F4B7X6_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Ahos_0767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7X6"/>
    <dbReference type="STRING" id="933801.Ahos_0767"/>
    <dbReference type="GeneID" id="10600226"/>
    <dbReference type="KEGG" id="aho:Ahos_0767"/>
    <dbReference type="eggNOG" id="arCOG02231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046973_1_0_2"/>
    <dbReference type="OrthoDB" id="4240at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028348">
        <property type="entry name" value="FAD-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049516">
        <property type="entry name" value="FAD-depend_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43106">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43106:SF1">
        <property type="entry name" value="DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21688">
        <property type="entry name" value="FAD-depend_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038984">
        <property type="entry name" value="FAD_binding_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent protein C-terminal" evidence="1">
        <location>
            <begin position="257"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="466" mass="51413" checksum="3FB7D7B139F2206F" modified="2011-06-28" version="1">MYDVVIIGGGPAGLFAAYELANALQGKSDYKILLVDKGVRASKRSCPLLTPKEKCTFCNPCHINYGIGGAGTFSSGIINLRPDIGGDLHELMRSWDAAQQLINYVDEIFVKFGAPKDRLFKPDPEKTKEIQKKAAKVGAEFVPIVQRHIGTDKSPQVIENLTNYIEQNGIKISELTDVYQIEKTGNSFNLKTSKGEIESRIVLVAPGRAGAKWFYEQTKRLGVDTVPGPLDIGVRVEVESFVMDELTSAVWDPKIIMYSKKYDDKVRTFCVNPRGFIMKEVYDDGTIGVNGETYVDRKSNNTNFAFLATIKLSDPLEDTIEYGKSIARLMTRLGGEKPILQRLIDFEKGRRSTWERINRSTVKPTLRDVTPGDISMGLPYRIISDLVEGLERLDNIASGIYSSNTLLYAPEIKYYSVKTVVDSNMETVVDNLFVAGDGAGLSRGINVAAATGILAARGIINKLGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>F4B7Z2</accession>
    <name>F4B7Z2_ACIHW</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">16S rRNA aminocarboxypropyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Ahos_0783</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B7Z2"/>
    <dbReference type="STRING" id="933801.Ahos_0783"/>
    <dbReference type="KEGG" id="aho:Ahos_0783"/>
    <dbReference type="eggNOG" id="arCOG04733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035060_4_1_2"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
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            <begin position="16"/>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AEE93669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="147" mass="17116" checksum="9D8248DEB132A0C3" modified="2011-06-28" version="1">MVRFGFAKYTEKPRGIVLNPLSEEVISIDDVEIVNKYGLSVIDSSWNKSDAKFYARFMSRFSRRLPLLFAGNPINYAKPYKLSSLEAVSASLYILNFIDISLKLLSLYKWGKTFYDLNKELLESYRGKHKDEIIEIEKSFLKEEPQQ</sequence>
</entry>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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            <dbReference type="EMBL" id="AEE93764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30039" checksum="904A93677A8D66C7" modified="2011-06-28" version="1">MRKISIGIMGSYTIDEIHENNIIYEKAGGSPIYSSLGVYIAGGEPHVYSIKGEDFKFELPDYIKEDFILKTKNNLKFEIIINNGKRTLKLKSPGSKIDFNKIGPLGNIDGLIINPVCNEIDLDQINVLNIPLAVDFQGIVRKCETGKEITYKEINYIPYKPNYMVAHANIEEKNNGKLDIKKLLDVGFKEIIISYGDLGFEIYNKNGVLKETVENRGNYEVGNGDFLLGFYFTLRVKGVKIEEAARLSKLASQKFSIYGPNLQPLLS</sequence>
</entry>
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    <accession>F4B8M5</accession>
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        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8M5"/>
    <dbReference type="STRING" id="933801.Ahos_2106"/>
    <dbReference type="GeneID" id="10601606"/>
    <dbReference type="KEGG" id="aho:Ahos_2106"/>
    <dbReference type="eggNOG" id="arCOG01943">
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    </dbReference>
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            <begin position="20"/>
            <end position="189"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00857"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE94977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="198" mass="22591" checksum="EAD2F2E9ECC19333" modified="2011-06-28" version="1">MVEVPKIPEEKEIELNPHDTALIIVDMQNDFVRKDGKLPVPTAENTIKPIKDLIKKARDSSALVIYTQDWHMKDDPEFKIWGEHALAGTWGAEIIDELKPEKDDFIIKKYRYDAFFETPLDYILRVKGIKNLIITGTVANICVLHTAGSAALRWYNVIMPKDGISAITDFDYYATLRQVDFLYKGKITTSAGIKFNTK</sequence>
</entry>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8P3"/>
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    <dbReference type="GeneID" id="10601625"/>
    <dbReference type="KEGG" id="aho:Ahos_2124"/>
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    </dbReference>
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        <property type="entry name" value="BLR3518 PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR37691:SF1">
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    </dbReference>
    <dbReference type="Pfam" id="PF02635">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="110" mass="12012" checksum="A294295E4550B7C9" modified="2011-06-28" version="1">MYKVIVQVKDEERVPQAIGSVINLYNDLKGDAEIEVVFHQSAIKALVKGNPNEDYVKKLLSSGINVVGCMNSINALNLNVESLIKGISIVQAGVGEIVRKQAEGWIYLSL</sequence>
</entry>
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    <accession>F4B8U4</accession>
    <name>F4B8U4_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AbrB family</fullName>
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    <organism evidence="2 3">
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
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                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B8U4"/>
    <dbReference type="GeneID" id="10600400"/>
    <dbReference type="KEGG" id="aho:Ahos_0928"/>
    <dbReference type="eggNOG" id="arCOG00817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3075520_0_0_2"/>
    <dbReference type="OrthoDB" id="30861at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052975">
        <property type="entry name" value="Repressor-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34860:SF6">
        <property type="entry name" value="REPRESSOR-LIKE PROTEIN SSO7C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="AbrB/MazE/MraZ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AEE93814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="53" mass="6089" checksum="AE341E299D566F6C" modified="2011-06-28" version="1">MATEDIVKVSRNYQVTIPARIRQKFQVKEGDLVKVVYDEKENAVKIIPANKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>F4B910</accession>
    <name>F4B910_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von Willebrand factor type A domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Ahos_2162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013776948.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B910"/>
    <dbReference type="STRING" id="933801.Ahos_2162"/>
    <dbReference type="GeneID" id="10601665"/>
    <dbReference type="KEGG" id="aho:Ahos_2162"/>
    <dbReference type="eggNOG" id="arCOG00442">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_598029_0_0_2"/>
    <dbReference type="OrthoDB" id="64524at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36846">
        <property type="entry name" value="PROTEIN VIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36846:SF1">
        <property type="entry name" value="PROTEIN VIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="289"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AEE95033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="452" mass="50536" checksum="C29953449942583D" modified="2011-06-28" version="1">MTGFLIGIDYDDPIVKYRGDRILYTLKKISGKDSNIDPDFLVDTYYVHYLPLPILKSKSEISQVDAIKYALLDMTLSSDLVLKNRNYSIANSAVSMALSVSYIQNLIEELERIRRTSQSAEEREAAEQILNGLMKGSQGKEGGEQKEQQGQTTDATKKLLKQVHEKALSKAAEDANAVRSMQRIVGGNGAGTGSVLNFEGDVHEVLRLARNTEIKKILEFLSGIPKLGSLTKKKTTRYSKGELYGYEEGSDLERIVPSELAMPEDLFYVRLAESQLLLYQKQIKESLGPIYLLLDKSGSMDGEKILWAKAVALALYSRARRENRDFYLRFFDNIPYPLIKVIKNAKSKDVIKMIEYIGKIRGGGGTDISRSVISACEDIKEGHVKGVSEVIILTDGEDKIAETTVRRSLKDANATLISVMIRGDNADLRRISDTYLVVYKLDQNDLLKVVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9G1</accession>
    <name>F4B9G1_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_2236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE95107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013777022.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9G1"/>
    <dbReference type="GeneID" id="10601741"/>
    <dbReference type="KEGG" id="aho:Ahos_2236"/>
    <dbReference type="eggNOG" id="arCOG05929">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773284_0_0_2"/>
    <dbReference type="OrthoDB" id="35434at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE95107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="146" mass="16543" checksum="C0F4267E84E37A99" modified="2011-06-28" version="1">MKVKIKSILNVIGHEELYVIPIACNGKYVLGLNFFEDIEGGRVARFVLIMDKYGEINSIKVVEGDKGIVIAEGVRDDMDAVSKVIKIDRKMVTNRIPLFINIKVKSFPETQDRGIRGYENYIKRYGEIDPSKLKGVIKLDVIEEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-06-28" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>F4B9M5</accession>
    <name>F4B9M5_ACIHW</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Ahos_1057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Acidianus hospitalis (strain W1)</name>
        <dbReference type="NCBI Taxonomy" id="933801"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Thermoproteota</taxon>
            <taxon>Thermoprotei</taxon>
            <taxon>Sulfolobales</taxon>
            <taxon>Sulfolobaceae</taxon>
            <taxon>Acidianus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2011" name="Extremophiles" volume="15" first="487" last="497">
            <title>Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="She Q."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="21607549"/>
            <dbReference type="DOI" id="10.1007/s00792-011-0379-y"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">W1</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Extremophiles" volume="0" first="0" last="0">
            <title>Genomic analyses of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles.</title>
            <authorList>
                <person name="You X.Y."/>
                <person name="Liu C."/>
                <person name="Wang S.Y."/>
                <person name="Jiang C.Y."/>
                <person name="Shah S.A."/>
                <person name="Prangishvili D."/>
                <person name="Liu S.J."/>
                <person name="Garrett R.A."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>W1</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP002535">
        <property type="protein sequence ID" value="AEE93941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_013775857.1">
        <property type="nucleotide sequence ID" value="NC_015518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F4B9M5"/>
    <dbReference type="STRING" id="933801.Ahos_1057"/>
    <dbReference type="GeneID" id="10600531"/>
    <dbReference type="KEGG" id="aho:Ahos_1057"/>
    <dbReference type="eggNOG" id="arCOG03639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086262_0_0_2"/>
    <dbReference type="OrthoDB" id="350529at2157"/>
    <dbReference type="Proteomes" id="UP000008458">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AEE93941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008458"/>
        </source>
    </evidence>
    <sequence length="236" mass="27211" checksum="8D2A272518117654" modified="2011-06-28" version="1">MCRLLAFRDREKINGEVLKAFVNSARYDPFSPYKSHPDGWGFIIFVKKSGIWRSLHYRSPEPIFQDDLSALTSIKGDEILGIIHARKAGKRFLQGLTHAHPYHIRAGVYDLYFAHNGSISRNAFENPSLPYTDSYMFLLKIAEGVSKTNDVKESFRFAFNSLKEYSASLNSALLSYSEGEGPIIQIGYYYNKKEALDSSEEYFKLYIWRNYVFSSTIKYYLGNADNELVYGDIMYL</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>